sktl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sktl
Entrez Gene ID	37356
Full Name	skittles
Synonyms	25/17,CG9985,Dmel\CG9985,PIP5-kinase,PIP5K,PIP5K 57B6,Sktl,fam,l(2)05475,l(2)k12405,ski
General protein information	Preferred Names skittles Names CG9985 gene product from transcript CG9985-RA CG9985-PA CG9985-PB CG9985-PC PIP5-kinase fata morgana phosphatidylinositol 4-phosphate 5-kinase sktl-PA sktl-PB sktl-PC type I phosphatidylinositol 4-phosphate 5-kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57B3-57B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299717.1	NP_001286646.1	skittles, isoform B
NM_001299718.1	NP_001286647.1	skittles, isoform C
NM_057858.4	NP_477206.1	skittles, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pip5k1ba	NP_001005775.1
Drosophila melanogaster (fruit fly)	sktl	NP_477206.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results provide evidence for the existence of a unique dPIP5K dependent pool of PIP2 required for normal Drosophila phototransduction.
Title: A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors.

The results indicate that the PIP5 kinase SKTL, by controlling PI(4,5)P2 polarity, regulates PAR-3 localization and thus the size of the apical domain.
Title: PI(4,5)P2 produced by the PI4P5K SKTL controls apical size by tethering PAR-3 in Drosophila epithelial cells.

Data show that mutation of the PIP(2) biosynthetic enzyme Skittles (Sktl) results in dramatic defects in spermatid cysts.
Title: Phosphatidylinositol 4,5-bisphosphate directs spermatid cell polarity and exocyst localization in Drosophila.

Skittles, by controlling the localization of Bazooka, Par-1 and Lgl, but not Lkb1, to the cell membrane, regulates PAR polarity proteins and the maintenance of specific cortical domains along the anteroposterior axis.
Title: PIP5K-dependent production of PIP2 sustains microtubule organization to establish polarized transport in the Drosophila oocyte.

The following sktl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sktl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10709	NM_057858.4 Latest version!	Drosophila melanogaster skittles (sktl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10709	NM_001299717.1 Latest version!	Drosophila melanogaster skittles (sktl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10709	NM_001299718.1 Latest version!	Drosophila melanogaster skittles (sktl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sktl ( NM_001299717.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286646.1 Drosophila melanogaster skittles (sktl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10709
Clone ID Related Accession (Same CDS sequence)	NM_001299717.1 , NM_001299718.1 , NM_057858.4
Accession Version	NM_001299717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	skittles, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCC GCGTGGAACT GGAACTGGAG CCGGTTGGCA AACAGGATAG GCTAAAGGAT 
CAGCCGGATG ATGAGAATGA ACCGCTGGAA AACCAGCTGG AGCTGGAGCA AGAGCACAAG 
AGCCTGCTCA CGCCGGTCAA GATTCCCCCG GCTCAGACCG CAAGTCCCGA CCAGGAGGCC 
ACGCCCAGCA CCCCACCACC CATGAGCCTG CAGCCGGGAA CATCTCGGCA GTTGTTCAGG 
GATGCCGCTA TGGCGCACGG AGGCCAGGAG ACCACATCGT TGCTGCAGCA GGAACTGAAC 
AACATAGCCG CCACCCAAAC GCCGGCCTCG AAACTCAGAA GCGCAAGCTC CACGCTGGAT 
GCCAGTATAT CCCGGAATCC CTCCACCACG GGCGGGAAGC ACGAGAAGAA GCTGGGCCAC 
CGGCGAGTGG CGGAGGGCGG TGAGGTGACC TACAAGAAGA TCCAATCCAA GCAAATCATG 
GGCTCCATTC AGCTGGGCAT CCAGCATACG GTGGGCAGCC TGGCCAGTAA GCCCAAGCGG 
GATCTGCTAA TGAATGACTT CTGGGAAATG GAAACGATTT CGTTTCCGCC AGATGGTTCC 
TCCATAACAC CTGCCCATCA CTACAACGAC TTCCGCTTCA AGGTCTACGC ACCCATCGCC 
TTTCGCTACT TCCGAGATCT GTTCGGAATC GCACCCGATG ACTTCCTCAT GTCCATGTGT 
GCGTCTCCCC TGAGGGAGCT TTCCAATCCG GGTGCCTCTG GTTCCATATT TTACCTGACG 
ACCGACGACG AGTTCATTAT CAAGACGGTG CAGAAGAAGG AATGCGAGTT CCTGCAAAAA 
CTTTTGCCCG GTTATTACAT GAACCTATCG CAGAATCCTC GAACGCTGCT GCCTAAGTTC 
TTCGGACTAT ATTGCTTCCA CTACAACTCG AAGAACGTGC GGTTGGTGGC CATGAACAAC 
CTGTTGCCTT CAGACATTAA GATGCACTGC AAGTACGACC TTAAGGGATC TTCCTTCCGA 
CGGAAGGCCT CCAAGGCAGA GCGACAAAAG GCGAGTCCCA CGTTCAAGGA CCTCGACTTT 
GCTGAGCACC ATCCGAATGG CATATTCCTG GAGACGGACA AGTACAATGC ACTGATGAGC 
ACCATTAAGC GGGACTGTAT GGTGCTGGAG AGTTTTCAGA TTATGGACTA CTCGCTGTTG 
GTGGGAATCC ACAACCTGGA CCTGGCCGCC AAGGAGAAAC GCGAAGAACG AATCCTAAAT 
GCGCGAGCTA AGCTGCAGCG CAAGGAAAGC GCCGCTCAGG GACCACCTTC CTTAAATCCG 
GACGACGATG CTCCGGAAGC GGATCAGAAT CAGCTCCAAG CGGTGGCCTC ATACGCCTCC 
ATACCGGGAA CTTCGGCTGG AGCTGCCCTG AATCGAACGC GCAGTATGAA TCGTCAGCGT 
TTGGTGGCCC ATTCCACTGC ACTGGAGTCC ATTACGGCTG ATATGGATGT GCCGCTCGAG 
GAGGACGAGG ATGTACCCGC CGGCGGCATT CCGGCTCGTT CTGAAAACGA CGAGCGGTTG 
ATCTTGTACA TCGGCATCAT CGACATTTTG CAGTCGTATC GCCTGGAAAA GAAGCTGGAG 
CACACATTCA AAAGCATTCT GTACAACGGA GACACAGTGT CCGTGTGCCG ACCCTCCTTC 
TACGCCAAGC GCTTCCAGGA CGCCATGGGC AAACAGGTGT TCAAGAAGAC GCCCACATTT 
CCACTGAAAC ATTCCCCCTC CAAGCGCAAA ACCTCGACCA CGCAGCTGCG CTCGCCGGCA 
TCGCGCTTAC CGCCATTGAG TACACCAACA AATGCAGTTC GTCAGCCGAT CATGACGATG 
TCGGGCATGT CGACGCCGCC GCCGGCCTTC GATGACATCT CGGAGGAGGA CATAACCGCC 
GCTTCCACCA GCTCGCTCCA GCAGCAGAGA AGCAGCAATC AGTCGAACAA CAATCGGGGC 
GAGACGGAGG TGGCCCTCAG GGAAGGGAAC AACACGAGAG GAGGCAGCGG AGAGCCCAGC 
AGCACCTATC ACACTCAATA CTCGTACGAC AGCTCCGGGC GAACAGGAAG CGCCTTGACC 
AGCGACTACA GCGACGATGA GTCCACGGGA ACGGATCTGA GCTCGCGGTT GCCCAAGCTG 
CGCACCCACC GGCTAACAAA GACGAGTGTT CAGGTCACGA CGGTTGGCTA TGTAGAGGGG 
GTGGACACGC CGCCGAAGGG CGGGCAGGAT GTGGTGCATG CAGATGTCAA CGGCAAGACC 
ACCATGACGA CCACCACCAT GCTGACGACG ACAAAGACGG CACACATCCA GGCGCCCAGC 
TACACGTCCA CGCTCGTGCT CAACGATTTG CCGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286646.1
CDS	659..3037
Translation	MDTRVELELEPVGKQDRLKDQPDDENEPLENQLELEQEHKSLLTPVKIPPAQTASPDQEATPSTPPPMSLQPGTSRQLFRDAAMAHGGQETTSLLQQELNNIAATQTPASKLRSASSTLDASISRNPSTTGGKHEKKLGHRRVAEGGEVTYKKIQSKQIMGSIQLGIQHTVGSLASKPKRDLLMNDFWEMETISFPPDGSSITPAHHYNDFRFKVYAPIAFRYFRDLFGIAPDDFLMSMCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLLPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNALMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGKQVFKKTPTFPLKHSPSKRKTSTTQLRSPASRLPPLSTPTNAVRQPIMTMSGMSTPPPAFDDISEEDITAASTSSLQQQRSSNQSNNNRGETEVALREGNNTRGGSGEPSSTYHTQYSYDSSGRTGSALTSDYSDDESTGTDLSSRLPKLRTHRLTKTSVQVTTVGYVEGVDTPPKGGQDVVHADVNGKTTMTTTTMLTTTKTAHIQAPSYTSTLVLNDLPR

Target ORF information:

RefSeq Version	NM_001299717.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster skittles (sktl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299717.1

ORF Insert Sequence:

ATGGACACCC GCGTGGAACT GGAACTGGAG CCGGTTGGCA AACAGGATAG GCTAAAGGAT 
CAGCCGGATG ATGAGAATGA ACCGCTGGAA AACCAGCTGG AGCTGGAGCA AGAGCACAAG 
AGCCTGCTCA CGCCGGTCAA GATTCCCCCG GCTCAGACCG CAAGTCCCGA CCAGGAGGCC 
ACGCCCAGCA CCCCACCACC CATGAGCCTG CAGCCGGGAA CATCTCGGCA GTTGTTCAGG 
GATGCCGCTA TGGCGCACGG AGGCCAGGAG ACCACATCGT TGCTGCAGCA GGAACTGAAC 
AACATAGCCG CCACCCAAAC GCCGGCCTCG AAACTCAGAA GCGCAAGCTC CACGCTGGAT 
GCCAGTATAT CCCGGAATCC CTCCACCACG GGCGGGAAGC ACGAGAAGAA GCTGGGCCAC 
CGGCGAGTGG CGGAGGGCGG TGAGGTGACC TACAAGAAGA TCCAATCCAA GCAAATCATG 
GGCTCCATTC AGCTGGGCAT CCAGCATACG GTGGGCAGCC TGGCCAGTAA GCCCAAGCGG 
GATCTGCTAA TGAATGACTT CTGGGAAATG GAAACGATTT CGTTTCCGCC AGATGGTTCC 
TCCATAACAC CTGCCCATCA CTACAACGAC TTCCGCTTCA AGGTCTACGC ACCCATCGCC 
TTTCGCTACT TCCGAGATCT GTTCGGAATC GCACCCGATG ACTTCCTCAT GTCCATGTGT 
GCGTCTCCCC TGAGGGAGCT TTCCAATCCG GGTGCCTCTG GTTCCATATT TTACCTGACG 
ACCGACGACG AGTTCATTAT CAAGACGGTG CAGAAGAAGG AATGCGAGTT CCTGCAAAAA 
CTTTTGCCCG GTTATTACAT GAACCTATCG CAGAATCCTC GAACGCTGCT GCCTAAGTTC 
TTCGGACTAT ATTGCTTCCA CTACAACTCG AAGAACGTGC GGTTGGTGGC CATGAACAAC 
CTGTTGCCTT CAGACATTAA GATGCACTGC AAGTACGACC TTAAGGGATC TTCCTTCCGA 
CGGAAGGCCT CCAAGGCAGA GCGACAAAAG GCGAGTCCCA CGTTCAAGGA CCTCGACTTT 
GCTGAGCACC ATCCGAATGG CATATTCCTG GAGACGGACA AGTACAATGC ACTGATGAGC 
ACCATTAAGC GGGACTGTAT GGTGCTGGAG AGTTTTCAGA TTATGGACTA CTCGCTGTTG 
GTGGGAATCC ACAACCTGGA CCTGGCCGCC AAGGAGAAAC GCGAAGAACG AATCCTAAAT 
GCGCGAGCTA AGCTGCAGCG CAAGGAAAGC GCCGCTCAGG GACCACCTTC CTTAAATCCG 
GACGACGATG CTCCGGAAGC GGATCAGAAT CAGCTCCAAG CGGTGGCCTC ATACGCCTCC 
ATACCGGGAA CTTCGGCTGG AGCTGCCCTG AATCGAACGC GCAGTATGAA TCGTCAGCGT 
TTGGTGGCCC ATTCCACTGC ACTGGAGTCC ATTACGGCTG ATATGGATGT GCCGCTCGAG 
GAGGACGAGG ATGTACCCGC CGGCGGCATT CCGGCTCGTT CTGAAAACGA CGAGCGGTTG 
ATCTTGTACA TCGGCATCAT CGACATTTTG CAGTCGTATC GCCTGGAAAA GAAGCTGGAG 
CACACATTCA AAAGCATTCT GTACAACGGA GACACAGTGT CCGTGTGCCG ACCCTCCTTC 
TACGCCAAGC GCTTCCAGGA CGCCATGGGC AAACAGGTGT TCAAGAAGAC GCCCACATTT 
CCACTGAAAC ATTCCCCCTC CAAGCGCAAA ACCTCGACCA CGCAGCTGCG CTCGCCGGCA 
TCGCGCTTAC CGCCATTGAG TACACCAACA AATGCAGTTC GTCAGCCGAT CATGACGATG 
TCGGGCATGT CGACGCCGCC GCCGGCCTTC GATGACATCT CGGAGGAGGA CATAACCGCC 
GCTTCCACCA GCTCGCTCCA GCAGCAGAGA AGCAGCAATC AGTCGAACAA CAATCGGGGC 
GAGACGGAGG TGGCCCTCAG GGAAGGGAAC AACACGAGAG GAGGCAGCGG AGAGCCCAGC 
AGCACCTATC ACACTCAATA CTCGTACGAC AGCTCCGGGC GAACAGGAAG CGCCTTGACC 
AGCGACTACA GCGACGATGA GTCCACGGGA ACGGATCTGA GCTCGCGGTT GCCCAAGCTG 
CGCACCCACC GGCTAACAAA GACGAGTGTT CAGGTCACGA CGGTTGGCTA TGTAGAGGGG 
GTGGACACGC CGCCGAAGGG CGGGCAGGAT GTGGTGCATG CAGATGTCAA CGGCAAGACC 
ACCATGACGA CCACCACCAT GCTGACGACG ACAAAGACGG CACACATCCA GGCGCCCAGC 
TACACGTCCA CGCTCGTGCT CAACGATTTG CCGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sktl ( NM_001299718.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286647.1 Drosophila melanogaster skittles (sktl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10709
Clone ID Related Accession (Same CDS sequence)	NM_001299717.1 , NM_001299718.1 , NM_057858.4
Accession Version	NM_001299718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	skittles, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCC GCGTGGAACT GGAACTGGAG CCGGTTGGCA AACAGGATAG GCTAAAGGAT 
CAGCCGGATG ATGAGAATGA ACCGCTGGAA AACCAGCTGG AGCTGGAGCA AGAGCACAAG 
AGCCTGCTCA CGCCGGTCAA GATTCCCCCG GCTCAGACCG CAAGTCCCGA CCAGGAGGCC 
ACGCCCAGCA CCCCACCACC CATGAGCCTG CAGCCGGGAA CATCTCGGCA GTTGTTCAGG 
GATGCCGCTA TGGCGCACGG AGGCCAGGAG ACCACATCGT TGCTGCAGCA GGAACTGAAC 
AACATAGCCG CCACCCAAAC GCCGGCCTCG AAACTCAGAA GCGCAAGCTC CACGCTGGAT 
GCCAGTATAT CCCGGAATCC CTCCACCACG GGCGGGAAGC ACGAGAAGAA GCTGGGCCAC 
CGGCGAGTGG CGGAGGGCGG TGAGGTGACC TACAAGAAGA TCCAATCCAA GCAAATCATG 
GGCTCCATTC AGCTGGGCAT CCAGCATACG GTGGGCAGCC TGGCCAGTAA GCCCAAGCGG 
GATCTGCTAA TGAATGACTT CTGGGAAATG GAAACGATTT CGTTTCCGCC AGATGGTTCC 
TCCATAACAC CTGCCCATCA CTACAACGAC TTCCGCTTCA AGGTCTACGC ACCCATCGCC 
TTTCGCTACT TCCGAGATCT GTTCGGAATC GCACCCGATG ACTTCCTCAT GTCCATGTGT 
GCGTCTCCCC TGAGGGAGCT TTCCAATCCG GGTGCCTCTG GTTCCATATT TTACCTGACG 
ACCGACGACG AGTTCATTAT CAAGACGGTG CAGAAGAAGG AATGCGAGTT CCTGCAAAAA 
CTTTTGCCCG GTTATTACAT GAACCTATCG CAGAATCCTC GAACGCTGCT GCCTAAGTTC 
TTCGGACTAT ATTGCTTCCA CTACAACTCG AAGAACGTGC GGTTGGTGGC CATGAACAAC 
CTGTTGCCTT CAGACATTAA GATGCACTGC AAGTACGACC TTAAGGGATC TTCCTTCCGA 
CGGAAGGCCT CCAAGGCAGA GCGACAAAAG GCGAGTCCCA CGTTCAAGGA CCTCGACTTT 
GCTGAGCACC ATCCGAATGG CATATTCCTG GAGACGGACA AGTACAATGC ACTGATGAGC 
ACCATTAAGC GGGACTGTAT GGTGCTGGAG AGTTTTCAGA TTATGGACTA CTCGCTGTTG 
GTGGGAATCC ACAACCTGGA CCTGGCCGCC AAGGAGAAAC GCGAAGAACG AATCCTAAAT 
GCGCGAGCTA AGCTGCAGCG CAAGGAAAGC GCCGCTCAGG GACCACCTTC CTTAAATCCG 
GACGACGATG CTCCGGAAGC GGATCAGAAT CAGCTCCAAG CGGTGGCCTC ATACGCCTCC 
ATACCGGGAA CTTCGGCTGG AGCTGCCCTG AATCGAACGC GCAGTATGAA TCGTCAGCGT 
TTGGTGGCCC ATTCCACTGC ACTGGAGTCC ATTACGGCTG ATATGGATGT GCCGCTCGAG 
GAGGACGAGG ATGTACCCGC CGGCGGCATT CCGGCTCGTT CTGAAAACGA CGAGCGGTTG 
ATCTTGTACA TCGGCATCAT CGACATTTTG CAGTCGTATC GCCTGGAAAA GAAGCTGGAG 
CACACATTCA AAAGCATTCT GTACAACGGA GACACAGTGT CCGTGTGCCG ACCCTCCTTC 
TACGCCAAGC GCTTCCAGGA CGCCATGGGC AAACAGGTGT TCAAGAAGAC GCCCACATTT 
CCACTGAAAC ATTCCCCCTC CAAGCGCAAA ACCTCGACCA CGCAGCTGCG CTCGCCGGCA 
TCGCGCTTAC CGCCATTGAG TACACCAACA AATGCAGTTC GTCAGCCGAT CATGACGATG 
TCGGGCATGT CGACGCCGCC GCCGGCCTTC GATGACATCT CGGAGGAGGA CATAACCGCC 
GCTTCCACCA GCTCGCTCCA GCAGCAGAGA AGCAGCAATC AGTCGAACAA CAATCGGGGC 
GAGACGGAGG TGGCCCTCAG GGAAGGGAAC AACACGAGAG GAGGCAGCGG AGAGCCCAGC 
AGCACCTATC ACACTCAATA CTCGTACGAC AGCTCCGGGC GAACAGGAAG CGCCTTGACC 
AGCGACTACA GCGACGATGA GTCCACGGGA ACGGATCTGA GCTCGCGGTT GCCCAAGCTG 
CGCACCCACC GGCTAACAAA GACGAGTGTT CAGGTCACGA CGGTTGGCTA TGTAGAGGGG 
GTGGACACGC CGCCGAAGGG CGGGCAGGAT GTGGTGCATG CAGATGTCAA CGGCAAGACC 
ACCATGACGA CCACCACCAT GCTGACGACG ACAAAGACGG CACACATCCA GGCGCCCAGC 
TACACGTCCA CGCTCGTGCT CAACGATTTG CCGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286647.1
CDS	383..2761
Translation	MDTRVELELEPVGKQDRLKDQPDDENEPLENQLELEQEHKSLLTPVKIPPAQTASPDQEATPSTPPPMSLQPGTSRQLFRDAAMAHGGQETTSLLQQELNNIAATQTPASKLRSASSTLDASISRNPSTTGGKHEKKLGHRRVAEGGEVTYKKIQSKQIMGSIQLGIQHTVGSLASKPKRDLLMNDFWEMETISFPPDGSSITPAHHYNDFRFKVYAPIAFRYFRDLFGIAPDDFLMSMCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLLPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNALMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGKQVFKKTPTFPLKHSPSKRKTSTTQLRSPASRLPPLSTPTNAVRQPIMTMSGMSTPPPAFDDISEEDITAASTSSLQQQRSSNQSNNNRGETEVALREGNNTRGGSGEPSSTYHTQYSYDSSGRTGSALTSDYSDDESTGTDLSSRLPKLRTHRLTKTSVQVTTVGYVEGVDTPPKGGQDVVHADVNGKTTMTTTTMLTTTKTAHIQAPSYTSTLVLNDLPR

Target ORF information:

RefSeq Version	NM_001299718.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster skittles (sktl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299718.1

ORF Insert Sequence:

ATGGACACCC GCGTGGAACT GGAACTGGAG CCGGTTGGCA AACAGGATAG GCTAAAGGAT 
CAGCCGGATG ATGAGAATGA ACCGCTGGAA AACCAGCTGG AGCTGGAGCA AGAGCACAAG 
AGCCTGCTCA CGCCGGTCAA GATTCCCCCG GCTCAGACCG CAAGTCCCGA CCAGGAGGCC 
ACGCCCAGCA CCCCACCACC CATGAGCCTG CAGCCGGGAA CATCTCGGCA GTTGTTCAGG 
GATGCCGCTA TGGCGCACGG AGGCCAGGAG ACCACATCGT TGCTGCAGCA GGAACTGAAC 
AACATAGCCG CCACCCAAAC GCCGGCCTCG AAACTCAGAA GCGCAAGCTC CACGCTGGAT 
GCCAGTATAT CCCGGAATCC CTCCACCACG GGCGGGAAGC ACGAGAAGAA GCTGGGCCAC 
CGGCGAGTGG CGGAGGGCGG TGAGGTGACC TACAAGAAGA TCCAATCCAA GCAAATCATG 
GGCTCCATTC AGCTGGGCAT CCAGCATACG GTGGGCAGCC TGGCCAGTAA GCCCAAGCGG 
GATCTGCTAA TGAATGACTT CTGGGAAATG GAAACGATTT CGTTTCCGCC AGATGGTTCC 
TCCATAACAC CTGCCCATCA CTACAACGAC TTCCGCTTCA AGGTCTACGC ACCCATCGCC 
TTTCGCTACT TCCGAGATCT GTTCGGAATC GCACCCGATG ACTTCCTCAT GTCCATGTGT 
GCGTCTCCCC TGAGGGAGCT TTCCAATCCG GGTGCCTCTG GTTCCATATT TTACCTGACG 
ACCGACGACG AGTTCATTAT CAAGACGGTG CAGAAGAAGG AATGCGAGTT CCTGCAAAAA 
CTTTTGCCCG GTTATTACAT GAACCTATCG CAGAATCCTC GAACGCTGCT GCCTAAGTTC 
TTCGGACTAT ATTGCTTCCA CTACAACTCG AAGAACGTGC GGTTGGTGGC CATGAACAAC 
CTGTTGCCTT CAGACATTAA GATGCACTGC AAGTACGACC TTAAGGGATC TTCCTTCCGA 
CGGAAGGCCT CCAAGGCAGA GCGACAAAAG GCGAGTCCCA CGTTCAAGGA CCTCGACTTT 
GCTGAGCACC ATCCGAATGG CATATTCCTG GAGACGGACA AGTACAATGC ACTGATGAGC 
ACCATTAAGC GGGACTGTAT GGTGCTGGAG AGTTTTCAGA TTATGGACTA CTCGCTGTTG 
GTGGGAATCC ACAACCTGGA CCTGGCCGCC AAGGAGAAAC GCGAAGAACG AATCCTAAAT 
GCGCGAGCTA AGCTGCAGCG CAAGGAAAGC GCCGCTCAGG GACCACCTTC CTTAAATCCG 
GACGACGATG CTCCGGAAGC GGATCAGAAT CAGCTCCAAG CGGTGGCCTC ATACGCCTCC 
ATACCGGGAA CTTCGGCTGG AGCTGCCCTG AATCGAACGC GCAGTATGAA TCGTCAGCGT 
TTGGTGGCCC ATTCCACTGC ACTGGAGTCC ATTACGGCTG ATATGGATGT GCCGCTCGAG 
GAGGACGAGG ATGTACCCGC CGGCGGCATT CCGGCTCGTT CTGAAAACGA CGAGCGGTTG 
ATCTTGTACA TCGGCATCAT CGACATTTTG CAGTCGTATC GCCTGGAAAA GAAGCTGGAG 
CACACATTCA AAAGCATTCT GTACAACGGA GACACAGTGT CCGTGTGCCG ACCCTCCTTC 
TACGCCAAGC GCTTCCAGGA CGCCATGGGC AAACAGGTGT TCAAGAAGAC GCCCACATTT 
CCACTGAAAC ATTCCCCCTC CAAGCGCAAA ACCTCGACCA CGCAGCTGCG CTCGCCGGCA 
TCGCGCTTAC CGCCATTGAG TACACCAACA AATGCAGTTC GTCAGCCGAT CATGACGATG 
TCGGGCATGT CGACGCCGCC GCCGGCCTTC GATGACATCT CGGAGGAGGA CATAACCGCC 
GCTTCCACCA GCTCGCTCCA GCAGCAGAGA AGCAGCAATC AGTCGAACAA CAATCGGGGC 
GAGACGGAGG TGGCCCTCAG GGAAGGGAAC AACACGAGAG GAGGCAGCGG AGAGCCCAGC 
AGCACCTATC ACACTCAATA CTCGTACGAC AGCTCCGGGC GAACAGGAAG CGCCTTGACC 
AGCGACTACA GCGACGATGA GTCCACGGGA ACGGATCTGA GCTCGCGGTT GCCCAAGCTG 
CGCACCCACC GGCTAACAAA GACGAGTGTT CAGGTCACGA CGGTTGGCTA TGTAGAGGGG 
GTGGACACGC CGCCGAAGGG CGGGCAGGAT GTGGTGCATG CAGATGTCAA CGGCAAGACC 
ACCATGACGA CCACCACCAT GCTGACGACG ACAAAGACGG CACACATCCA GGCGCCCAGC 
TACACGTCCA CGCTCGTGCT CAACGATTTG CCGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sktl ( NM_057858.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477206.1 Drosophila melanogaster skittles (sktl), transcript variant A, mRNA.

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CloneID	OFa10709
Clone ID Related Accession (Same CDS sequence)	NM_001299717.1 , NM_001299718.1 , NM_057858.4
Accession Version	NM_057858.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	skittles, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057858.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCC GCGTGGAACT GGAACTGGAG CCGGTTGGCA AACAGGATAG GCTAAAGGAT 
CAGCCGGATG ATGAGAATGA ACCGCTGGAA AACCAGCTGG AGCTGGAGCA AGAGCACAAG 
AGCCTGCTCA CGCCGGTCAA GATTCCCCCG GCTCAGACCG CAAGTCCCGA CCAGGAGGCC 
ACGCCCAGCA CCCCACCACC CATGAGCCTG CAGCCGGGAA CATCTCGGCA GTTGTTCAGG 
GATGCCGCTA TGGCGCACGG AGGCCAGGAG ACCACATCGT TGCTGCAGCA GGAACTGAAC 
AACATAGCCG CCACCCAAAC GCCGGCCTCG AAACTCAGAA GCGCAAGCTC CACGCTGGAT 
GCCAGTATAT CCCGGAATCC CTCCACCACG GGCGGGAAGC ACGAGAAGAA GCTGGGCCAC 
CGGCGAGTGG CGGAGGGCGG TGAGGTGACC TACAAGAAGA TCCAATCCAA GCAAATCATG 
GGCTCCATTC AGCTGGGCAT CCAGCATACG GTGGGCAGCC TGGCCAGTAA GCCCAAGCGG 
GATCTGCTAA TGAATGACTT CTGGGAAATG GAAACGATTT CGTTTCCGCC AGATGGTTCC 
TCCATAACAC CTGCCCATCA CTACAACGAC TTCCGCTTCA AGGTCTACGC ACCCATCGCC 
TTTCGCTACT TCCGAGATCT GTTCGGAATC GCACCCGATG ACTTCCTCAT GTCCATGTGT 
GCGTCTCCCC TGAGGGAGCT TTCCAATCCG GGTGCCTCTG GTTCCATATT TTACCTGACG 
ACCGACGACG AGTTCATTAT CAAGACGGTG CAGAAGAAGG AATGCGAGTT CCTGCAAAAA 
CTTTTGCCCG GTTATTACAT GAACCTATCG CAGAATCCTC GAACGCTGCT GCCTAAGTTC 
TTCGGACTAT ATTGCTTCCA CTACAACTCG AAGAACGTGC GGTTGGTGGC CATGAACAAC 
CTGTTGCCTT CAGACATTAA GATGCACTGC AAGTACGACC TTAAGGGATC TTCCTTCCGA 
CGGAAGGCCT CCAAGGCAGA GCGACAAAAG GCGAGTCCCA CGTTCAAGGA CCTCGACTTT 
GCTGAGCACC ATCCGAATGG CATATTCCTG GAGACGGACA AGTACAATGC ACTGATGAGC 
ACCATTAAGC GGGACTGTAT GGTGCTGGAG AGTTTTCAGA TTATGGACTA CTCGCTGTTG 
GTGGGAATCC ACAACCTGGA CCTGGCCGCC AAGGAGAAAC GCGAAGAACG AATCCTAAAT 
GCGCGAGCTA AGCTGCAGCG CAAGGAAAGC GCCGCTCAGG GACCACCTTC CTTAAATCCG 
GACGACGATG CTCCGGAAGC GGATCAGAAT CAGCTCCAAG CGGTGGCCTC ATACGCCTCC 
ATACCGGGAA CTTCGGCTGG AGCTGCCCTG AATCGAACGC GCAGTATGAA TCGTCAGCGT 
TTGGTGGCCC ATTCCACTGC ACTGGAGTCC ATTACGGCTG ATATGGATGT GCCGCTCGAG 
GAGGACGAGG ATGTACCCGC CGGCGGCATT CCGGCTCGTT CTGAAAACGA CGAGCGGTTG 
ATCTTGTACA TCGGCATCAT CGACATTTTG CAGTCGTATC GCCTGGAAAA GAAGCTGGAG 
CACACATTCA AAAGCATTCT GTACAACGGA GACACAGTGT CCGTGTGCCG ACCCTCCTTC 
TACGCCAAGC GCTTCCAGGA CGCCATGGGC AAACAGGTGT TCAAGAAGAC GCCCACATTT 
CCACTGAAAC ATTCCCCCTC CAAGCGCAAA ACCTCGACCA CGCAGCTGCG CTCGCCGGCA 
TCGCGCTTAC CGCCATTGAG TACACCAACA AATGCAGTTC GTCAGCCGAT CATGACGATG 
TCGGGCATGT CGACGCCGCC GCCGGCCTTC GATGACATCT CGGAGGAGGA CATAACCGCC 
GCTTCCACCA GCTCGCTCCA GCAGCAGAGA AGCAGCAATC AGTCGAACAA CAATCGGGGC 
GAGACGGAGG TGGCCCTCAG GGAAGGGAAC AACACGAGAG GAGGCAGCGG AGAGCCCAGC 
AGCACCTATC ACACTCAATA CTCGTACGAC AGCTCCGGGC GAACAGGAAG CGCCTTGACC 
AGCGACTACA GCGACGATGA GTCCACGGGA ACGGATCTGA GCTCGCGGTT GCCCAAGCTG 
CGCACCCACC GGCTAACAAA GACGAGTGTT CAGGTCACGA CGGTTGGCTA TGTAGAGGGG 
GTGGACACGC CGCCGAAGGG CGGGCAGGAT GTGGTGCATG CAGATGTCAA CGGCAAGACC 
ACCATGACGA CCACCACCAT GCTGACGACG ACAAAGACGG CACACATCCA GGCGCCCAGC 
TACACGTCCA CGCTCGTGCT CAACGATTTG CCGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477206.1
CDS	659..3037
Translation	MDTRVELELEPVGKQDRLKDQPDDENEPLENQLELEQEHKSLLTPVKIPPAQTASPDQEATPSTPPPMSLQPGTSRQLFRDAAMAHGGQETTSLLQQELNNIAATQTPASKLRSASSTLDASISRNPSTTGGKHEKKLGHRRVAEGGEVTYKKIQSKQIMGSIQLGIQHTVGSLASKPKRDLLMNDFWEMETISFPPDGSSITPAHHYNDFRFKVYAPIAFRYFRDLFGIAPDDFLMSMCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLLPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNALMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGKQVFKKTPTFPLKHSPSKRKTSTTQLRSPASRLPPLSTPTNAVRQPIMTMSGMSTPPPAFDDISEEDITAASTSSLQQQRSSNQSNNNRGETEVALREGNNTRGGSGEPSSTYHTQYSYDSSGRTGSALTSDYSDDESTGTDLSSRLPKLRTHRLTKTSVQVTTVGYVEGVDTPPKGGQDVVHADVNGKTTMTTTTMLTTTKTAHIQAPSYTSTLVLNDLPR

Target ORF information:

RefSeq Version	NM_057858.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster skittles (sktl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057858.4

ORF Insert Sequence:

ATGGACACCC GCGTGGAACT GGAACTGGAG CCGGTTGGCA AACAGGATAG GCTAAAGGAT 
CAGCCGGATG ATGAGAATGA ACCGCTGGAA AACCAGCTGG AGCTGGAGCA AGAGCACAAG 
AGCCTGCTCA CGCCGGTCAA GATTCCCCCG GCTCAGACCG CAAGTCCCGA CCAGGAGGCC 
ACGCCCAGCA CCCCACCACC CATGAGCCTG CAGCCGGGAA CATCTCGGCA GTTGTTCAGG 
GATGCCGCTA TGGCGCACGG AGGCCAGGAG ACCACATCGT TGCTGCAGCA GGAACTGAAC 
AACATAGCCG CCACCCAAAC GCCGGCCTCG AAACTCAGAA GCGCAAGCTC CACGCTGGAT 
GCCAGTATAT CCCGGAATCC CTCCACCACG GGCGGGAAGC ACGAGAAGAA GCTGGGCCAC 
CGGCGAGTGG CGGAGGGCGG TGAGGTGACC TACAAGAAGA TCCAATCCAA GCAAATCATG 
GGCTCCATTC AGCTGGGCAT CCAGCATACG GTGGGCAGCC TGGCCAGTAA GCCCAAGCGG 
GATCTGCTAA TGAATGACTT CTGGGAAATG GAAACGATTT CGTTTCCGCC AGATGGTTCC 
TCCATAACAC CTGCCCATCA CTACAACGAC TTCCGCTTCA AGGTCTACGC ACCCATCGCC 
TTTCGCTACT TCCGAGATCT GTTCGGAATC GCACCCGATG ACTTCCTCAT GTCCATGTGT 
GCGTCTCCCC TGAGGGAGCT TTCCAATCCG GGTGCCTCTG GTTCCATATT TTACCTGACG 
ACCGACGACG AGTTCATTAT CAAGACGGTG CAGAAGAAGG AATGCGAGTT CCTGCAAAAA 
CTTTTGCCCG GTTATTACAT GAACCTATCG CAGAATCCTC GAACGCTGCT GCCTAAGTTC 
TTCGGACTAT ATTGCTTCCA CTACAACTCG AAGAACGTGC GGTTGGTGGC CATGAACAAC 
CTGTTGCCTT CAGACATTAA GATGCACTGC AAGTACGACC TTAAGGGATC TTCCTTCCGA 
CGGAAGGCCT CCAAGGCAGA GCGACAAAAG GCGAGTCCCA CGTTCAAGGA CCTCGACTTT 
GCTGAGCACC ATCCGAATGG CATATTCCTG GAGACGGACA AGTACAATGC ACTGATGAGC 
ACCATTAAGC GGGACTGTAT GGTGCTGGAG AGTTTTCAGA TTATGGACTA CTCGCTGTTG 
GTGGGAATCC ACAACCTGGA CCTGGCCGCC AAGGAGAAAC GCGAAGAACG AATCCTAAAT 
GCGCGAGCTA AGCTGCAGCG CAAGGAAAGC GCCGCTCAGG GACCACCTTC CTTAAATCCG 
GACGACGATG CTCCGGAAGC GGATCAGAAT CAGCTCCAAG CGGTGGCCTC ATACGCCTCC 
ATACCGGGAA CTTCGGCTGG AGCTGCCCTG AATCGAACGC GCAGTATGAA TCGTCAGCGT 
TTGGTGGCCC ATTCCACTGC ACTGGAGTCC ATTACGGCTG ATATGGATGT GCCGCTCGAG 
GAGGACGAGG ATGTACCCGC CGGCGGCATT CCGGCTCGTT CTGAAAACGA CGAGCGGTTG 
ATCTTGTACA TCGGCATCAT CGACATTTTG CAGTCGTATC GCCTGGAAAA GAAGCTGGAG 
CACACATTCA AAAGCATTCT GTACAACGGA GACACAGTGT CCGTGTGCCG ACCCTCCTTC 
TACGCCAAGC GCTTCCAGGA CGCCATGGGC AAACAGGTGT TCAAGAAGAC GCCCACATTT 
CCACTGAAAC ATTCCCCCTC CAAGCGCAAA ACCTCGACCA CGCAGCTGCG CTCGCCGGCA 
TCGCGCTTAC CGCCATTGAG TACACCAACA AATGCAGTTC GTCAGCCGAT CATGACGATG 
TCGGGCATGT CGACGCCGCC GCCGGCCTTC GATGACATCT CGGAGGAGGA CATAACCGCC 
GCTTCCACCA GCTCGCTCCA GCAGCAGAGA AGCAGCAATC AGTCGAACAA CAATCGGGGC 
GAGACGGAGG TGGCCCTCAG GGAAGGGAAC AACACGAGAG GAGGCAGCGG AGAGCCCAGC 
AGCACCTATC ACACTCAATA CTCGTACGAC AGCTCCGGGC GAACAGGAAG CGCCTTGACC 
AGCGACTACA GCGACGATGA GTCCACGGGA ACGGATCTGA GCTCGCGGTT GCCCAAGCTG 
CGCACCCACC GGCTAACAAA GACGAGTGTT CAGGTCACGA CGGTTGGCTA TGTAGAGGGG 
GTGGACACGC CGCCGAAGGG CGGGCAGGAT GTGGTGCATG CAGATGTCAA CGGCAAGACC 
ACCATGACGA CCACCACCAT GCTGACGACG ACAAAGACGG CACACATCCA GGCGCCCAGC 
TACACGTCCA CGCTCGTGCT CAACGATTTG CCGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sktl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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