PCNA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCNA
Entrez Gene ID	37290
Full Name	Proliferating cell nuclear antigen
Synonyms	53/13,CG9193,DmPCNA,Dmel\CG9193,MUS209,Mus209,PCNA/mus209,Pcna,dPCNA,l(2)02448,l(2)56F,l(2)56Fa,l(2)s1534,mus 209,mus209,pcna
General protein information	Preferred Names Proliferating cell nuclear antigen Names CG9193 gene product from transcript CG9193-RA CG9193-PA CG9193-PB PCNA-PA PCNA-PB Proliferating-cell-nuclear-antigen mutagen-sensitive 209 proliferating cell nuclear antigen proliferating-cell nuclear antigen
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56F11-56F11\|2-92.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_206182.2	NP_995904.1	proliferating cell nuclear antigen, isoform B
NM_057557.4	NP_476905.1	proliferating cell nuclear antigen, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PCNA	NP_001029666.1
Mus musculus (house mouse)	Pcna	NP_035175.1
Rattus norvegicus (Norway rat)	Pcna	NP_071776.1
Drosophila melanogaster (fruit fly)	mus209	NP_995904.1
Caenorhabditis elegans (roundworm)	pcn-1	NP_500466.3
Schizosaccharomyces pombe (fission yeast)	pcn1	NP_596504.1
	PCNA	XP_001115746.1
Gallus gallus (chicken)	PCNA	NP_989501.1
Danio rerio (zebrafish)	pcna	XP_005155472.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010220	XP_319407.2
Arabidopsis thaliana (thale cress)	PCNA2	NP_180517.1
Canis lupus familiaris (dog)	PCNA	XP_534355.3
Saccharomyces cerevisiae (baker's yeast)	POL30	NP_009645.1
Arabidopsis thaliana (thale cress)	PCNA1	NP_172217.1
Pan troglodytes (chimpanzee)	PCNA	XP_001165515.1
Homo sapiens (human)	PCNA	NP_872590.1
Kluyveromyces lactis	KLLA0B12078g	XP_452067.1
Eremothecium gossypii	AGOS_ADL309W	NP_983787.2
Magnaporthe oryzae (rice blast fungus)	MGG_00518	XP_003718539.1
Neurospora crassa	NCU09239	XP_964674.1
Oryza sativa (rice)	Os02g0805200	NP_001048438.1
Xenopus tropicalis (tropical clawed frog)	pcna	NP_001007921.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that regulation of e2f1 and PCNA by DREF in vivo is complex and the regulation mechanism may differ with the tissue and/or positions in the tissue.
Title: Control of e2f1 and PCNA by Drosophila transcription factor DREF.

crystal of DmPCNA diffracted to 2.0 A resolution and belonged to space group H3, with unit-cell parameters a = b = 151.16, c = 38.28 A
Title: Structure of PCNA from Drosophila melanogaster.

We observed that SUUR chromosomal localization changed along with PCNA pattern and these proteins largely co-localized during the late S-phase in salivary glands.
Title: Drosophila SUUR protein associates with PCNA and binds chromatin in a cell cycle-dependent manner.

A Q123V mutation dramaticly enhances the abilities of Drosophila PCNA to stimulate calf thymus pol delta. Replacing the entire interdomain connector loop AA 119-133 with the corresponding residues from human PCNA results in additional enhancement.
Title: Site-specific mutagenesis of Drosophila proliferating cell nuclear antigen enhances its effects on calf thymus DNA polymerase delta.

E2F regulation of PCNA is dispensable for viability, but is nonetheless important for normal Drosophila development.
Title: The contribution of E2F-regulated transcription to Drosophila PCNA gene function.

The following PCNA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCNA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13117	NM_206182.2 Latest version!	Drosophila melanogaster proliferating cell nuclear antigen, transcript variant B (PCNA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa13117	NM_057557.4 Latest version!	Drosophila melanogaster proliferating cell nuclear antigen, transcript variant A (PCNA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCNA ( NM_206182.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995904.1 Drosophila melanogaster proliferating cell nuclear antigen, transcript variant B (PCNA), mRNA.

Price & Availability↑

CloneID	OFa13117
Clone ID Related Accession (Same CDS sequence)	NM_206182.2 , NM_057557.4
Accession Version	NM_206182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	proliferating cell nuclear antigen, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_206182.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAGG CACGCCTGGG TCAAGCCACC ATCCTGAAGA AGATCTTGGA TGCCATCAAG 
GATCTGCTCA ATGAGGCAAC CTTCGATTGC AGCGACTCCG GCATTCAGCT ACAGGCCATG 
GACAACTCCC ATGTGTCGCT TGTCTCGCTG ACCCTGCGTT CCGATGGCTT CGACAAGTTT 
CGCTGCGACC GCAATCTCTC CATGGGCATG AATCTGGGCA GCATGGCCAA GATTCTGAAA 
TGCGCCAACA ACGAGGACAA TGTGACGATG AAGGCGCAGG ATAACGCCGA CACTGTCACC 
ATCATGTTCG AATCGGCTAA CCAGGAGAAG GTATCGGACT ACGAGATGAA ACTGATGAAC 
CTCGACCAGG AGCACCTGGG CATACCGGAG ACAGACTTCT CGTGCGTGGT CCGCATGCCG 
GCCATGGAGT TCGCTCGCAT CTGCCGCGAT CTGGCGCAGT TCAGCGAATC CGTTGTGATC 
TGCTGCACCA AGGAGGGCGT CAAGTTCTCG GCCAGCGGCG ATGTGGGCAC CGCCAACATT 
AAGCTAGCCC AAACCGGCTC TGTCGACAAG GAGGAGGAGG CGGTGATCAT CGAGATGCAG 
GAGCCGGTGA CGCTGACATT TGCCTGTCGC TACCTGAACG CCTTCACAAA GGCGACGCCA 
TTGTCCACCC AAGTGCAGCT GTCGATGTGC GCAGATGTTC CGCTGGTAGT CGAGTATGCG 
ATCAAGGATC TGGGTCACAT TCGCTACTAC CTGGCACCCA AGATCGAGGA CAACGAGACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995904.1
CDS	90..872
Translation	MFEARLGQATILKKILDAIKDLLNEATFDCSDSGIQLQAMDNSHVSLVSLTLRSDGFDKFRCDRNLSMGMNLGSMAKILKCANNEDNVTMKAQDNADTVTIMFESANQEKVSDYEMKLMNLDQEHLGIPETDFSCVVRMPAMEFARICRDLAQFSESVVICCTKEGVKFSASGDVGTANIKLAQTGSVDKEEEAVIIEMQEPVTLTFACRYLNAFTKATPLSTQVQLSMCADVPLVVEYAIKDLGHIRYYLAPKIEDNET

Target ORF information:

RefSeq Version	NM_206182.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proliferating cell nuclear antigen, transcript variant B (PCNA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206182.2

ORF Insert Sequence:

ATGTTCGAGG CACGCCTGGG TCAAGCCACC ATCCTGAAGA AGATCTTGGA TGCCATCAAG 
GATCTGCTCA ATGAGGCAAC CTTCGATTGC AGCGACTCCG GCATTCAGCT ACAGGCCATG 
GACAACTCCC ATGTGTCGCT TGTCTCGCTG ACCCTGCGTT CCGATGGCTT CGACAAGTTT 
CGCTGCGACC GCAATCTCTC CATGGGCATG AATCTGGGCA GCATGGCCAA GATTCTGAAA 
TGCGCCAACA ACGAGGACAA TGTGACGATG AAGGCGCAGG ATAACGCCGA CACTGTCACC 
ATCATGTTCG AATCGGCTAA CCAGGAGAAG GTATCGGACT ACGAGATGAA ACTGATGAAC 
CTCGACCAGG AGCACCTGGG CATACCGGAG ACAGACTTCT CGTGCGTGGT CCGCATGCCG 
GCCATGGAGT TCGCTCGCAT CTGCCGCGAT CTGGCGCAGT TCAGCGAATC CGTTGTGATC 
TGCTGCACCA AGGAGGGCGT CAAGTTCTCG GCCAGCGGCG ATGTGGGCAC CGCCAACATT 
AAGCTAGCCC AAACCGGCTC TGTCGACAAG GAGGAGGAGG CGGTGATCAT CGAGATGCAG 
GAGCCGGTGA CGCTGACATT TGCCTGTCGC TACCTGAACG CCTTCACAAA GGCGACGCCA 
TTGTCCACCC AAGTGCAGCT GTCGATGTGC GCAGATGTTC CGCTGGTAGT CGAGTATGCG 
ATCAAGGATC TGGGTCACAT TCGCTACTAC CTGGCACCCA AGATCGAGGA CAACGAGACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCNA ( NM_057557.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476905.1 Drosophila melanogaster proliferating cell nuclear antigen, transcript variant A (PCNA), mRNA.

Price & Availability↑

CloneID	OFa13117
Clone ID Related Accession (Same CDS sequence)	NM_206182.2 , NM_057557.4
Accession Version	NM_057557.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	proliferating cell nuclear antigen, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057557.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAGG CACGCCTGGG TCAAGCCACC ATCCTGAAGA AGATCTTGGA TGCCATCAAG 
GATCTGCTCA ATGAGGCAAC CTTCGATTGC AGCGACTCCG GCATTCAGCT ACAGGCCATG 
GACAACTCCC ATGTGTCGCT TGTCTCGCTG ACCCTGCGTT CCGATGGCTT CGACAAGTTT 
CGCTGCGACC GCAATCTCTC CATGGGCATG AATCTGGGCA GCATGGCCAA GATTCTGAAA 
TGCGCCAACA ACGAGGACAA TGTGACGATG AAGGCGCAGG ATAACGCCGA CACTGTCACC 
ATCATGTTCG AATCGGCTAA CCAGGAGAAG GTATCGGACT ACGAGATGAA ACTGATGAAC 
CTCGACCAGG AGCACCTGGG CATACCGGAG ACAGACTTCT CGTGCGTGGT CCGCATGCCG 
GCCATGGAGT TCGCTCGCAT CTGCCGCGAT CTGGCGCAGT TCAGCGAATC CGTTGTGATC 
TGCTGCACCA AGGAGGGCGT CAAGTTCTCG GCCAGCGGCG ATGTGGGCAC CGCCAACATT 
AAGCTAGCCC AAACCGGCTC TGTCGACAAG GAGGAGGAGG CGGTGATCAT CGAGATGCAG 
GAGCCGGTGA CGCTGACATT TGCCTGTCGC TACCTGAACG CCTTCACAAA GGCGACGCCA 
TTGTCCACCC AAGTGCAGCT GTCGATGTGC GCAGATGTTC CGCTGGTAGT CGAGTATGCG 
ATCAAGGATC TGGGTCACAT TCGCTACTAC CTGGCACCCA AGATCGAGGA CAACGAGACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476905.1
CDS	163..945
Translation	MFEARLGQATILKKILDAIKDLLNEATFDCSDSGIQLQAMDNSHVSLVSLTLRSDGFDKFRCDRNLSMGMNLGSMAKILKCANNEDNVTMKAQDNADTVTIMFESANQEKVSDYEMKLMNLDQEHLGIPETDFSCVVRMPAMEFARICRDLAQFSESVVICCTKEGVKFSASGDVGTANIKLAQTGSVDKEEEAVIIEMQEPVTLTFACRYLNAFTKATPLSTQVQLSMCADVPLVVEYAIKDLGHIRYYLAPKIEDNET

Target ORF information:

RefSeq Version	NM_057557.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proliferating cell nuclear antigen, transcript variant A (PCNA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057557.4

ORF Insert Sequence:

ATGTTCGAGG CACGCCTGGG TCAAGCCACC ATCCTGAAGA AGATCTTGGA TGCCATCAAG 
GATCTGCTCA ATGAGGCAAC CTTCGATTGC AGCGACTCCG GCATTCAGCT ACAGGCCATG 
GACAACTCCC ATGTGTCGCT TGTCTCGCTG ACCCTGCGTT CCGATGGCTT CGACAAGTTT 
CGCTGCGACC GCAATCTCTC CATGGGCATG AATCTGGGCA GCATGGCCAA GATTCTGAAA 
TGCGCCAACA ACGAGGACAA TGTGACGATG AAGGCGCAGG ATAACGCCGA CACTGTCACC 
ATCATGTTCG AATCGGCTAA CCAGGAGAAG GTATCGGACT ACGAGATGAA ACTGATGAAC 
CTCGACCAGG AGCACCTGGG CATACCGGAG ACAGACTTCT CGTGCGTGGT CCGCATGCCG 
GCCATGGAGT TCGCTCGCAT CTGCCGCGAT CTGGCGCAGT TCAGCGAATC CGTTGTGATC 
TGCTGCACCA AGGAGGGCGT CAAGTTCTCG GCCAGCGGCG ATGTGGGCAC CGCCAACATT 
AAGCTAGCCC AAACCGGCTC TGTCGACAAG GAGGAGGAGG CGGTGATCAT CGAGATGCAG 
GAGCCGGTGA CGCTGACATT TGCCTGTCGC TACCTGAACG CCTTCACAAA GGCGACGCCA 
TTGTCCACCC AAGTGCAGCT GTCGATGTGC GCAGATGTTC CGCTGGTAGT CGAGTATGCG 
ATCAAGGATC TGGGTCACAT TCGCTACTAC CTGGCACCCA AGATCGAGGA CAACGAGACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PCNA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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