hpo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hpo
Entrez Gene ID	37247
Full Name	hippo
Synonyms	CG11228,Dmel\CG11228,Hippo,Hpo,MST,MST2,dMST,hipo
General protein information	Preferred Names hippo Names CG11228 gene product from transcript CG11228-RB CG11228-PA CG11228-PB MST hippo kinase hpo-PA hpo-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56D13-56D13

mRNA and Protein(s)

mRNA	Protein	Name
NM_137583.5	NP_611427.1	hippo, isoform A
NM_001274163.1	NP_001261092.1	hippo, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	STK3	XP_003951359.1
Danio rerio (zebrafish)	stk3	NP_955966.1
Xenopus tropicalis (tropical clawed frog)	stk3	NP_001090665.1
Canis lupus familiaris (dog)	STK3	XP_532280.2
Rattus norvegicus (Norway rat)	Stk3	NP_113923.1
Bos taurus (cattle)	STK3	NP_001073075.1
	STK3	XP_001095834.2
Drosophila melanogaster (fruit fly)	hpo	NP_611427.1
Caenorhabditis elegans (roundworm)	cst-1	NP_001024575.2
Homo sapiens (human)	STK3	NP_001243241.1
Mus musculus (house mouse)	Stk3	NP_062609.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006044	XP_316092.4
Caenorhabditis elegans (roundworm)	cst-2	NP_001041219.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this work, we have explored the disc autonomous function of TGFbeta that promotes wing imaginal disc growth. We have studied the genetic interactions between TGFbeta signaling and other pathways regulating wing disc growth, such as the Insulin and Hippo/Salvador/Warts pathways, as well as cell cycle regulators.
Title: A Search for Genes Mediating the Growth-Promoting Function of TGF? in the

Merlin and Kibra activate Hippo signaling in parallel to Expanded at a spatially distinct cellular domain, the medial apical cortex.
Title: Kibra and Merlin Activate the Hippo Pathway Spatially Distinct from and Independent of Expanded.

Interaction with Slimb leads to the ubiquitination and degradation of dSmurf, resulting in negative regulation of dSmurf-mediated Yki phosphorylation and activity in the Hippo pathway. Thus our study revealed a new regulatory mechanism of the Hippo pathway which may provide implications for developing tumor treatment.
Title: The Drosophila F-box protein Slimb controls dSmurf protein turnover to regulate the Hippo pathway.

App promotes drosophila growth via the Hippo pathway by palmitoylation of Fat protein.
Title: The palmitoyltransferase Approximated promotes growth via the Hippo pathway by palmitoylation of Fat.

Vamana associates with the Dachsous intracellular domain and with a region of the Fat intracellular domain that is essential for controlling Hippo signaling and levels of Dachs.
Title: Vamana Couples Fat Signaling to the Hippo Pathway.

The following hpo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hpo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10646	NM_001274163.1 Latest version!	Drosophila melanogaster hippo (hpo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa10646	NM_137583.5 Latest version!	Drosophila melanogaster hippo (hpo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hpo ( NM_137583.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611427.1 Drosophila melanogaster hippo (hpo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10646
Clone ID Related Accession (Same CDS sequence)	NM_137583.5 , NM_001274163.1
Accession Version	NM_137583.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hippo, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137583.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGAGC CAGAGGTGAC CAGCGTTGTA GATATGAAAT CGCCCAACAT ATCCTCCTCC 
TGCTCCTTCT TCAAGCTGAA GAAGCTGTCG GAGGAGTCGC TTCTGCAGCC GCCGGAAAAG 
GTCTTCGACA TTATGTACAA GCTGGGCGAG GGCAGCTATG GATCAGTCTA CAAGGCAGTG 
CACAAGGAAA GCAGCTCCAT TGTGGCCATC AAGCTGGTGC CGGTGGAGTC CGACCTGCAC 
GAGATTATTA AGGAGATATC CATTATGCAA CAATGCGACT CGCCGTATGT GGTGCGCTAT 
TACGGCTCCT ACTTCAAGCA GTATGACCTG TGGATCTGCA TGGAGTACTG TGGCGCCGGC 
AGCGTCTCCG ACATTATGCG TCTGCGCAAA AAGACGCTGA CGGAGGACGA GATAGCCACT 
ATTCTGTCGG ACACTCTGCA GGGCTTGGTC TATCTGCATC TGCGTCGCAA GATCCATCGC 
GACATCAAGG CGGCCAACAT CCTGCTCAAT ACCGAAGGCT ATGCTAAGTT GGCCGATTTC 
GGAGTCGCTG GTCAGCTCAC GGACACAATG GCCAAGAGAA ACACTGTGAT CGGAACGCCC 
TTCTGGATGG CCCCCGAGGT GATTGAGGAG ATTGGCTACG ACTGTGTGGC AGACATATGG 
TCATTGGGCA TCACCGCCTT GGAAATGGCC GAGGGAAAGC CCCCATACGG TGAGATCCAT 
CCCATGCGAG CCATCTTTAT GATTCCGCAG AAGCCACCAC CATCGTTCCG GGAACCGGAT 
CGCTGGAGCA CAGAGTTCAT TGACTTCGTG AGCAAGTGCC TGGTAAAGGA GCCAGACGAC 
CGGGCAACGG CCACTGAACT GCTGGAGCAC GAGTTCATAC GCAACGCCAA GCACCGATCG 
ATCCTAAAGC CTATGCTTGA GGAGACCTGT GCCATTCGCG AGCAGCAACG CGCCAATCGC 
AGTTTTGGGG GCGTGTTGGC TGCTAGTCAG GCGAAGAGCT TGGCAACCCA GGAAAACGGA 
ATGCAACAAC ACATCACGGA CAACGCGTTT ATGGAGGATC CAGGTACTCT GGTGCCGGAG 
AAGTTCGGTG AATACCAACA GAGCTCAGCC TCAGATGCCA CCATGATAGC GCATGCAGAG 
CAAGGTGTGG ATGAGGGCAC TTTGGGGCCG GGTGGACTAA GGAACCTGTC CAAGGCAGCT 
GCTCCTGCAG CGGCCTCTTC GGCAGCATCT CCTCTGGACA TGCCGGCAGT TGACAGCGGC 
ACAATGGTGG AGCTGGAGTC GAACTTGGGC ACTATGGTAA TCAACTCCGA TTCGGACGAC 
TCCACAACGG CCAAAAACAA CGACGACCAG AAGCCGCGAA ACCGCTACAG GCCGCAGTTC 
CTGGAGCACT TCGATCGCAA AAATGCGGGA GATGGCCGTG GCGATGAAAA ACCCATAGCC 
ACAGAGTATT CTCCGGCAGC AGCGGAGCAG CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAGCAGG ATGAACAGCA TCTGGCAAGC GGGGCCAACG ATTTGAACAA CTGGGAGCAC 
AACATGGAAA TGCAGTTCCA GCAGATCTCC GCCATTAATC AGTATGGTCT GCAGCAGCAC 
CAGCAGCAGC AGCAAGTTCT GATGGCTTAT CCCCTGATGA ACGAACAACT CATCGCGCTC 
AACAATCAAC CGAATCTGCT GCTCAGCAAC GCTGCGCCAA TGGGACAGCA GGGAATACCG 
GCAGCGGCTC CAGCTCAACC GCCGCCCGCA TATCAGAATC AGCATATGCA TACGCAATCG 
CACGCCTATG TTGAGGGTGA GTTTGAGTTC CTCAAGTTCC TTACCTTCGA CGATCTGAAC 
CAGCGGCTGT GCAACATCGA TCACGAAATG GAGCTGGAGA TCGAACAGCT AAACAAGAAA 
TACAATGCCA AGCGGCAGCC AATTGTTGAC GCCATGAATG CAAAGCGCAA ACGCCAGCAG 
AACATCAATA ATAATCTGAT TAAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611427.1
CDS	109..2118
Translation	MSEPEVTSVVDMKSPNISSSCSFFKLKKLSEESLLQPPEKVFDIMYKLGEGSYGSVYKAVHKESSSIVAIKLVPVESDLHEIIKEISIMQQCDSPYVVRYYGSYFKQYDLWICMEYCGAGSVSDIMRLRKKTLTEDEIATILSDTLQGLVYLHLRRKIHRDIKAANILLNTEGYAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIEEIGYDCVADIWSLGITALEMAEGKPPYGEIHPMRAIFMIPQKPPPSFREPDRWSTEFIDFVSKCLVKEPDDRATATELLEHEFIRNAKHRSILKPMLEETCAIREQQRANRSFGGVLAASQAKSLATQENGMQQHITDNAFMEDPGTLVPEKFGEYQQSSASDATMIAHAEQGVDEGTLGPGGLRNLSKAAAPAAASSAASPLDMPAVDSGTMVELESNLGTMVINSDSDDSTTAKNNDDQKPRNRYRPQFLEHFDRKNAGDGRGDEKPIATEYSPAAAEQQQQQQQQQQQQQQDEQHLASGANDLNNWEHNMEMQFQQISAINQYGLQQHQQQQQVLMAYPLMNEQLIALNNQPNLLLSNAAPMGQQGIPAAAPAQPPPAYQNQHMHTQSHAYVEGEFEFLKFLTFDDLNQRLCNIDHEMELEIEQLNKKYNAKRQPIVDAMNAKRKRQQNINNNLIKI

Target ORF information:

RefSeq Version	NM_137583.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hippo (hpo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137583.5

ORF Insert Sequence:

ATGTCTGAGC CAGAGGTGAC CAGCGTTGTA GATATGAAAT CGCCCAACAT ATCCTCCTCC 
TGCTCCTTCT TCAAGCTGAA GAAGCTGTCG GAGGAGTCGC TTCTGCAGCC GCCGGAAAAG 
GTCTTCGACA TTATGTACAA GCTGGGCGAG GGCAGCTATG GATCAGTCTA CAAGGCAGTG 
CACAAGGAAA GCAGCTCCAT TGTGGCCATC AAGCTGGTGC CGGTGGAGTC CGACCTGCAC 
GAGATTATTA AGGAGATATC CATTATGCAA CAATGCGACT CGCCGTATGT GGTGCGCTAT 
TACGGCTCCT ACTTCAAGCA GTATGACCTG TGGATCTGCA TGGAGTACTG TGGCGCCGGC 
AGCGTCTCCG ACATTATGCG TCTGCGCAAA AAGACGCTGA CGGAGGACGA GATAGCCACT 
ATTCTGTCGG ACACTCTGCA GGGCTTGGTC TATCTGCATC TGCGTCGCAA GATCCATCGC 
GACATCAAGG CGGCCAACAT CCTGCTCAAT ACCGAAGGCT ATGCTAAGTT GGCCGATTTC 
GGAGTCGCTG GTCAGCTCAC GGACACAATG GCCAAGAGAA ACACTGTGAT CGGAACGCCC 
TTCTGGATGG CCCCCGAGGT GATTGAGGAG ATTGGCTACG ACTGTGTGGC AGACATATGG 
TCATTGGGCA TCACCGCCTT GGAAATGGCC GAGGGAAAGC CCCCATACGG TGAGATCCAT 
CCCATGCGAG CCATCTTTAT GATTCCGCAG AAGCCACCAC CATCGTTCCG GGAACCGGAT 
CGCTGGAGCA CAGAGTTCAT TGACTTCGTG AGCAAGTGCC TGGTAAAGGA GCCAGACGAC 
CGGGCAACGG CCACTGAACT GCTGGAGCAC GAGTTCATAC GCAACGCCAA GCACCGATCG 
ATCCTAAAGC CTATGCTTGA GGAGACCTGT GCCATTCGCG AGCAGCAACG CGCCAATCGC 
AGTTTTGGGG GCGTGTTGGC TGCTAGTCAG GCGAAGAGCT TGGCAACCCA GGAAAACGGA 
ATGCAACAAC ACATCACGGA CAACGCGTTT ATGGAGGATC CAGGTACTCT GGTGCCGGAG 
AAGTTCGGTG AATACCAACA GAGCTCAGCC TCAGATGCCA CCATGATAGC GCATGCAGAG 
CAAGGTGTGG ATGAGGGCAC TTTGGGGCCG GGTGGACTAA GGAACCTGTC CAAGGCAGCT 
GCTCCTGCAG CGGCCTCTTC GGCAGCATCT CCTCTGGACA TGCCGGCAGT TGACAGCGGC 
ACAATGGTGG AGCTGGAGTC GAACTTGGGC ACTATGGTAA TCAACTCCGA TTCGGACGAC 
TCCACAACGG CCAAAAACAA CGACGACCAG AAGCCGCGAA ACCGCTACAG GCCGCAGTTC 
CTGGAGCACT TCGATCGCAA AAATGCGGGA GATGGCCGTG GCGATGAAAA ACCCATAGCC 
ACAGAGTATT CTCCGGCAGC AGCGGAGCAG CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAGCAGG ATGAACAGCA TCTGGCAAGC GGGGCCAACG ATTTGAACAA CTGGGAGCAC 
AACATGGAAA TGCAGTTCCA GCAGATCTCC GCCATTAATC AGTATGGTCT GCAGCAGCAC 
CAGCAGCAGC AGCAAGTTCT GATGGCTTAT CCCCTGATGA ACGAACAACT CATCGCGCTC 
AACAATCAAC CGAATCTGCT GCTCAGCAAC GCTGCGCCAA TGGGACAGCA GGGAATACCG 
GCAGCGGCTC CAGCTCAACC GCCGCCCGCA TATCAGAATC AGCATATGCA TACGCAATCG 
CACGCCTATG TTGAGGGTGA GTTTGAGTTC CTCAAGTTCC TTACCTTCGA CGATCTGAAC 
CAGCGGCTGT GCAACATCGA TCACGAAATG GAGCTGGAGA TCGAACAGCT AAACAAGAAA 
TACAATGCCA AGCGGCAGCC AATTGTTGAC GCCATGAATG CAAAGCGCAA ACGCCAGCAG 
AACATCAATA ATAATCTGAT TAAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hpo ( NM_001274163.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261092.1 Drosophila melanogaster hippo (hpo), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10646
Clone ID Related Accession (Same CDS sequence)	NM_137583.5 , NM_001274163.1
Accession Version	NM_001274163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hippo, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGAGC CAGAGGTGAC CAGCGTTGTA GATATGAAAT CGCCCAACAT ATCCTCCTCC 
TGCTCCTTCT TCAAGCTGAA GAAGCTGTCG GAGGAGTCGC TTCTGCAGCC GCCGGAAAAG 
GTCTTCGACA TTATGTACAA GCTGGGCGAG GGCAGCTATG GATCAGTCTA CAAGGCAGTG 
CACAAGGAAA GCAGCTCCAT TGTGGCCATC AAGCTGGTGC CGGTGGAGTC CGACCTGCAC 
GAGATTATTA AGGAGATATC CATTATGCAA CAATGCGACT CGCCGTATGT GGTGCGCTAT 
TACGGCTCCT ACTTCAAGCA GTATGACCTG TGGATCTGCA TGGAGTACTG TGGCGCCGGC 
AGCGTCTCCG ACATTATGCG TCTGCGCAAA AAGACGCTGA CGGAGGACGA GATAGCCACT 
ATTCTGTCGG ACACTCTGCA GGGCTTGGTC TATCTGCATC TGCGTCGCAA GATCCATCGC 
GACATCAAGG CGGCCAACAT CCTGCTCAAT ACCGAAGGCT ATGCTAAGTT GGCCGATTTC 
GGAGTCGCTG GTCAGCTCAC GGACACAATG GCCAAGAGAA ACACTGTGAT CGGAACGCCC 
TTCTGGATGG CCCCCGAGGT GATTGAGGAG ATTGGCTACG ACTGTGTGGC AGACATATGG 
TCATTGGGCA TCACCGCCTT GGAAATGGCC GAGGGAAAGC CCCCATACGG TGAGATCCAT 
CCCATGCGAG CCATCTTTAT GATTCCGCAG AAGCCACCAC CATCGTTCCG GGAACCGGAT 
CGCTGGAGCA CAGAGTTCAT TGACTTCGTG AGCAAGTGCC TGGTAAAGGA GCCAGACGAC 
CGGGCAACGG CCACTGAACT GCTGGAGCAC GAGTTCATAC GCAACGCCAA GCACCGATCG 
ATCCTAAAGC CTATGCTTGA GGAGACCTGT GCCATTCGCG AGCAGCAACG CGCCAATCGC 
AGTTTTGGGG GCGTGTTGGC TGCTAGTCAG GCGAAGAGCT TGGCAACCCA GGAAAACGGA 
ATGCAACAAC ACATCACGGA CAACGCGTTT ATGGAGGATC CAGGTACTCT GGTGCCGGAG 
AAGTTCGGTG AATACCAACA GAGCTCAGCC TCAGATGCCA CCATGATAGC GCATGCAGAG 
CAAGGTGTGG ATGAGGGCAC TTTGGGGCCG GGTGGACTAA GGAACCTGTC CAAGGCAGCT 
GCTCCTGCAG CGGCCTCTTC GGCAGCATCT CCTCTGGACA TGCCGGCAGT TGACAGCGGC 
ACAATGGTGG AGCTGGAGTC GAACTTGGGC ACTATGGTAA TCAACTCCGA TTCGGACGAC 
TCCACAACGG CCAAAAACAA CGACGACCAG AAGCCGCGAA ACCGCTACAG GCCGCAGTTC 
CTGGAGCACT TCGATCGCAA AAATGCGGGA GATGGCCGTG GCGATGAAAA ACCCATAGCC 
ACAGAGTATT CTCCGGCAGC AGCGGAGCAG CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAGCAGG ATGAACAGCA TCTGGCAAGC GGGGCCAACG ATTTGAACAA CTGGGAGCAC 
AACATGGAAA TGCAGTTCCA GCAGATCTCC GCCATTAATC AGTATGGTCT GCAGCAGCAC 
CAGCAGCAGC AGCAAGTTCT GATGGCTTAT CCCCTGATGA ACGAACAACT CATCGCGCTC 
AACAATCAAC CGAATCTGCT GCTCAGCAAC GCTGCGCCAA TGGGACAGCA GGGAATACCG 
GCAGCGGCTC CAGCTCAACC GCCGCCCGCA TATCAGAATC AGCATATGCA TACGCAATCG 
CACGCCTATG TTGAGGGTGA GTTTGAGTTC CTCAAGTTCC TTACCTTCGA CGATCTGAAC 
CAGCGGCTGT GCAACATCGA TCACGAAATG GAGCTGGAGA TCGAACAGCT AAACAAGAAA 
TACAATGCCA AGCGGCAGCC AATTGTTGAC GCCATGAATG CAAAGCGCAA ACGCCAGCAG 
AACATCAATA ATAATCTGAT TAAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261092.1
CDS	109..2118
Translation	MSEPEVTSVVDMKSPNISSSCSFFKLKKLSEESLLQPPEKVFDIMYKLGEGSYGSVYKAVHKESSSIVAIKLVPVESDLHEIIKEISIMQQCDSPYVVRYYGSYFKQYDLWICMEYCGAGSVSDIMRLRKKTLTEDEIATILSDTLQGLVYLHLRRKIHRDIKAANILLNTEGYAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIEEIGYDCVADIWSLGITALEMAEGKPPYGEIHPMRAIFMIPQKPPPSFREPDRWSTEFIDFVSKCLVKEPDDRATATELLEHEFIRNAKHRSILKPMLEETCAIREQQRANRSFGGVLAASQAKSLATQENGMQQHITDNAFMEDPGTLVPEKFGEYQQSSASDATMIAHAEQGVDEGTLGPGGLRNLSKAAAPAAASSAASPLDMPAVDSGTMVELESNLGTMVINSDSDDSTTAKNNDDQKPRNRYRPQFLEHFDRKNAGDGRGDEKPIATEYSPAAAEQQQQQQQQQQQQQQDEQHLASGANDLNNWEHNMEMQFQQISAINQYGLQQHQQQQQVLMAYPLMNEQLIALNNQPNLLLSNAAPMGQQGIPAAAPAQPPPAYQNQHMHTQSHAYVEGEFEFLKFLTFDDLNQRLCNIDHEMELEIEQLNKKYNAKRQPIVDAMNAKRKRQQNINNNLIKI

Target ORF information:

RefSeq Version	NM_001274163.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hippo (hpo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274163.1

ORF Insert Sequence:

ATGTCTGAGC CAGAGGTGAC CAGCGTTGTA GATATGAAAT CGCCCAACAT ATCCTCCTCC 
TGCTCCTTCT TCAAGCTGAA GAAGCTGTCG GAGGAGTCGC TTCTGCAGCC GCCGGAAAAG 
GTCTTCGACA TTATGTACAA GCTGGGCGAG GGCAGCTATG GATCAGTCTA CAAGGCAGTG 
CACAAGGAAA GCAGCTCCAT TGTGGCCATC AAGCTGGTGC CGGTGGAGTC CGACCTGCAC 
GAGATTATTA AGGAGATATC CATTATGCAA CAATGCGACT CGCCGTATGT GGTGCGCTAT 
TACGGCTCCT ACTTCAAGCA GTATGACCTG TGGATCTGCA TGGAGTACTG TGGCGCCGGC 
AGCGTCTCCG ACATTATGCG TCTGCGCAAA AAGACGCTGA CGGAGGACGA GATAGCCACT 
ATTCTGTCGG ACACTCTGCA GGGCTTGGTC TATCTGCATC TGCGTCGCAA GATCCATCGC 
GACATCAAGG CGGCCAACAT CCTGCTCAAT ACCGAAGGCT ATGCTAAGTT GGCCGATTTC 
GGAGTCGCTG GTCAGCTCAC GGACACAATG GCCAAGAGAA ACACTGTGAT CGGAACGCCC 
TTCTGGATGG CCCCCGAGGT GATTGAGGAG ATTGGCTACG ACTGTGTGGC AGACATATGG 
TCATTGGGCA TCACCGCCTT GGAAATGGCC GAGGGAAAGC CCCCATACGG TGAGATCCAT 
CCCATGCGAG CCATCTTTAT GATTCCGCAG AAGCCACCAC CATCGTTCCG GGAACCGGAT 
CGCTGGAGCA CAGAGTTCAT TGACTTCGTG AGCAAGTGCC TGGTAAAGGA GCCAGACGAC 
CGGGCAACGG CCACTGAACT GCTGGAGCAC GAGTTCATAC GCAACGCCAA GCACCGATCG 
ATCCTAAAGC CTATGCTTGA GGAGACCTGT GCCATTCGCG AGCAGCAACG CGCCAATCGC 
AGTTTTGGGG GCGTGTTGGC TGCTAGTCAG GCGAAGAGCT TGGCAACCCA GGAAAACGGA 
ATGCAACAAC ACATCACGGA CAACGCGTTT ATGGAGGATC CAGGTACTCT GGTGCCGGAG 
AAGTTCGGTG AATACCAACA GAGCTCAGCC TCAGATGCCA CCATGATAGC GCATGCAGAG 
CAAGGTGTGG ATGAGGGCAC TTTGGGGCCG GGTGGACTAA GGAACCTGTC CAAGGCAGCT 
GCTCCTGCAG CGGCCTCTTC GGCAGCATCT CCTCTGGACA TGCCGGCAGT TGACAGCGGC 
ACAATGGTGG AGCTGGAGTC GAACTTGGGC ACTATGGTAA TCAACTCCGA TTCGGACGAC 
TCCACAACGG CCAAAAACAA CGACGACCAG AAGCCGCGAA ACCGCTACAG GCCGCAGTTC 
CTGGAGCACT TCGATCGCAA AAATGCGGGA GATGGCCGTG GCGATGAAAA ACCCATAGCC 
ACAGAGTATT CTCCGGCAGC AGCGGAGCAG CAACAACAAC AACAACAGCA GCAGCAACAA 
CAACAGCAGG ATGAACAGCA TCTGGCAAGC GGGGCCAACG ATTTGAACAA CTGGGAGCAC 
AACATGGAAA TGCAGTTCCA GCAGATCTCC GCCATTAATC AGTATGGTCT GCAGCAGCAC 
CAGCAGCAGC AGCAAGTTCT GATGGCTTAT CCCCTGATGA ACGAACAACT CATCGCGCTC 
AACAATCAAC CGAATCTGCT GCTCAGCAAC GCTGCGCCAA TGGGACAGCA GGGAATACCG 
GCAGCGGCTC CAGCTCAACC GCCGCCCGCA TATCAGAATC AGCATATGCA TACGCAATCG 
CACGCCTATG TTGAGGGTGA GTTTGAGTTC CTCAAGTTCC TTACCTTCGA CGATCTGAAC 
CAGCGGCTGT GCAACATCGA TCACGAAATG GAGCTGGAGA TCGAACAGCT AAACAAGAAA 
TACAATGCCA AGCGGCAGCC AATTGTTGAC GCCATGAATG CAAAGCGCAA ACGCCAGCAG 
AACATCAATA ATAATCTGAT TAAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
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hpo cDNA ORF clone, Drosophila melanogaster(fruit fly)

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