Fak cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fak
Entrez Gene ID	37233
Full Name	Focal adhesion kinase
Synonyms	CG10023,CT28129,DFAK,DFak,DFak56,Dfak,Dfak56,DmFAK,Dmel\CG10023,FAK,FAK65D,Fak56,Fak56D,dFak,fak,fak56,pFAK,ptk2
General protein information	Preferred Names Focal adhesion kinase Names CG10023 gene product from transcript CG10023-RG CG10023-PA CG10023-PB CG10023-PC CG10023-PD CG10023-PF CG10023-PG Fak-PA Fak-PB Fak-PC Fak-PD Fak-PF Fak-PG focal adhesion kinase focal adhesion kinase homolog protein tyrosine kinase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56D5-56D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_166352.3	NP_725891.1	focal adhesion kinase, isoform B
NM_001144246.3	NP_001137718.1	focal adhesion kinase, isoform D
NM_166353.3	NP_725892.1	focal adhesion kinase, isoform C
NM_079069.4	NP_523793.2	focal adhesion kinase, isoform A
NM_001169736.2	NP_001163207.1	focal adhesion kinase, isoform F
NM_001144247.3	NP_001137719.2	focal adhesion kinase, isoform G

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PTK2	NP_990766.1
Xenopus tropicalis (tropical clawed frog)	ptk2	XP_002934523.2
Homo sapiens (human)	PTK2	NP_005598.3
	PTK2	XP_001093060.2
Danio rerio (zebrafish)	ptk2ab	NP_571871.1
Pan troglodytes (chimpanzee)	PTK2	XP_519982.2
Mus musculus (house mouse)	Ptk2	NP_032008.2
Canis lupus familiaris (dog)	PTK2	XP_005628050.1
Rattus norvegicus (Norway rat)	Ptk2	NP_037213.1
Drosophila melanogaster (fruit fly)	Fak	NP_001137719.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011118	XP_309528.4
Bos taurus (cattle)	PTK2	NP_001068718.2
Caenorhabditis elegans (roundworm)	kin-32	NP_740841.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

presynaptic FAK signaling may be disrupted, causing abnormal synaptic growth and transmission in the NF1 genetic
Title: Neurofibromin mediates FAK signaling in confining synapse growth at Drosophila neuromuscular junctions.

Data show that Fak56 is required to restrict larval neuromuscular junctions (NMJ)growth during NMJ development and mediates an extracellular signal through the integrin receptor.
Title: Fak56 functions downstream of integrin alphaPS3betanu and suppresses MAPK activation in neuromuscular junction growth.

An intron loss of Dfak gene in species of the Drosophila melanogaster subgroup.
Title: An intron loss of Dfak gene in species of the Drosophila melanogaster subgroup and phylogenetic analysis.

Together these findings suggest that modulation of Fak56 function is important for action potential propagation and Ca2+-regulated neuromuscular transmission in vivo.
Title: Mutation of Drosophila focal adhesion kinase induces bang-sensitive behavior and disrupts glial function, axonal conduction and synaptic transmission.

Fak56D mutation causes severe disruption of the optic stalk structure. These phenotypes were completely rescued by Fak56D transgene expression in the SG cells but not in photoreceptor cells.
Title: Focal adhesion kinase controls morphogenesis of the Drosophila optic stalk.

The following Fak gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fak cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10635	NM_001144247.3 Latest version!	Drosophila melanogaster focal adhesion kinase, transcript variant G (Fak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10635	NM_166352.3 Latest version!	Drosophila melanogaster focal adhesion kinase, transcript variant B (Fak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10635	NM_079069.4 Latest version!	Drosophila melanogaster focal adhesion kinase, transcript variant A (Fak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10635	NM_166353.3 Latest version!	Drosophila melanogaster focal adhesion kinase, transcript variant C (Fak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa12137	NM_001169736.2 Latest version!	Drosophila melanogaster focal adhesion kinase, transcript variant F (Fak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa10636	NM_001144246.3 Latest version!	Drosophila melanogaster focal adhesion kinase, transcript variant D (Fak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fak ( NM_166352.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725891.1 Drosophila melanogaster focal adhesion kinase, transcript variant B (Fak), mRNA.

Price & Availability↑

CloneID	OFa10635
Clone ID Related Accession (Same CDS sequence)	NM_166352.3 , NM_166353.3 , NM_079069.4 , NM_001144247.3
Accession Version	NM_166352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	focal adhesion kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166352.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725891.1
CDS	450..4052
Translation	MNTAGATSQPPPTKNEINSEEYLIHVHMPNKSFKAVRFNVKETVFHVIRRTVEDLGTDGRTPSIQRYACRMLNMITKEVIWLARSTSMQKVLSHILTPGCSNVDCPNNQSELDEVLLEHGRRITDNRVWRVELRVRYVPNNIQELFEEDKATCFYYFNQVKEDFIQANVTAIDTEVAVQLCCLGIRHYFKNITVKAPDKKQHIDYIEKEIGFKSFLPQSVIATSKPKNLKKLIQVGYKKVYNYNDIEYLTRFFDLLKNIYLTNFEQFSVTLSSAWNISGILHVGPHIGISYQTHPQASLKNVAQFKDVVSIKTCTLPKEKLSKSGENTTEPELQNFNCNCQKIKTQIKISASNNVEDLVITCNGINTAESIADLIDGYCRLLSKDLEFTIWHRETNASNEDSAKALPNDATLGSNKSTSSQGKPMLTDDYAEIGLLEGEGDYSTPTVRNYELDRALITPSAKIGVGQFGDVYVGTYTLPKLGKGKNLAGNGKNSNSDQRNADSRPDVIQVAIKTCKANDDPEKTENFLAEAYIMQKFDHPHIIRLIGICSVMPIWIVMELAKLGELRAYLKTNSERLSHGTLLKYCYQLSTALSYLESKKFVHRDIAARNVLVSSPTCVKLADFGLSRWVSDQSYYHSTPTVALPIKWMSPESINFRRFTTASDVWMFGVCIWEILMLGVKPFQGVKNSDVILKLENGERLPLPPNCPPRLYSLMSQCWAYEPLKRPNFKRIKETLHEILIEDSINSSETLKREQRKVASMSWIGSDDIDIPPSKPSRVMHDPDITGLMPETTGLPQTYIIAQNPAVLAKLMMENQKRGINPAAYTTPASGIHNVLGEKLRQQQKDSNSDSEWLIQEELLRQRSCSIPQGSLNDHQAQMFKLDFMSAGPSSLPDCSNSSSRPMTPNANLSSLKSNHSSADHLSSLTSAEEQMGSNARNLGSAVPSRPPNRADDEVYCATTLVVKSIMALSQGVEKANTEGYLELVKNVGVKLRNLLTSVDKISIIFPAQALKEVQMAHQVLSKDMHELVSAMRLAQQYSDTTLDCEYRKSMLSAAHVLAMDAKNLFDVVDSIRQRYQHLFPPSATKETSCSSSFESTSGSIVAEPVNDLGGYIKTSTSGDLLQNTGIYDNDLHHSFNSQLQLQNPKASIDLSGGGSLQRGMSLGLDTTRSTNEPLRIVEETLGSPGEHMYCNTSALHGHA

Target ORF information:

RefSeq Version	NM_166352.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster focal adhesion kinase, transcript variant B (Fak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166352.3

ORF Insert Sequence:

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fak ( NM_166353.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725892.1 Drosophila melanogaster focal adhesion kinase, transcript variant C (Fak), mRNA.

Price & Availability↑

CloneID	OFa10635
Clone ID Related Accession (Same CDS sequence)	NM_166352.3 , NM_166353.3 , NM_079069.4 , NM_001144247.3
Accession Version	NM_166353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	focal adhesion kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166353.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725892.1
CDS	191..3793
Translation	MNTAGATSQPPPTKNEINSEEYLIHVHMPNKSFKAVRFNVKETVFHVIRRTVEDLGTDGRTPSIQRYACRMLNMITKEVIWLARSTSMQKVLSHILTPGCSNVDCPNNQSELDEVLLEHGRRITDNRVWRVELRVRYVPNNIQELFEEDKATCFYYFNQVKEDFIQANVTAIDTEVAVQLCCLGIRHYFKNITVKAPDKKQHIDYIEKEIGFKSFLPQSVIATSKPKNLKKLIQVGYKKVYNYNDIEYLTRFFDLLKNIYLTNFEQFSVTLSSAWNISGILHVGPHIGISYQTHPQASLKNVAQFKDVVSIKTCTLPKEKLSKSGENTTEPELQNFNCNCQKIKTQIKISASNNVEDLVITCNGINTAESIADLIDGYCRLLSKDLEFTIWHRETNASNEDSAKALPNDATLGSNKSTSSQGKPMLTDDYAEIGLLEGEGDYSTPTVRNYELDRALITPSAKIGVGQFGDVYVGTYTLPKLGKGKNLAGNGKNSNSDQRNADSRPDVIQVAIKTCKANDDPEKTENFLAEAYIMQKFDHPHIIRLIGICSVMPIWIVMELAKLGELRAYLKTNSERLSHGTLLKYCYQLSTALSYLESKKFVHRDIAARNVLVSSPTCVKLADFGLSRWVSDQSYYHSTPTVALPIKWMSPESINFRRFTTASDVWMFGVCIWEILMLGVKPFQGVKNSDVILKLENGERLPLPPNCPPRLYSLMSQCWAYEPLKRPNFKRIKETLHEILIEDSINSSETLKREQRKVASMSWIGSDDIDIPPSKPSRVMHDPDITGLMPETTGLPQTYIIAQNPAVLAKLMMENQKRGINPAAYTTPASGIHNVLGEKLRQQQKDSNSDSEWLIQEELLRQRSCSIPQGSLNDHQAQMFKLDFMSAGPSSLPDCSNSSSRPMTPNANLSSLKSNHSSADHLSSLTSAEEQMGSNARNLGSAVPSRPPNRADDEVYCATTLVVKSIMALSQGVEKANTEGYLELVKNVGVKLRNLLTSVDKISIIFPAQALKEVQMAHQVLSKDMHELVSAMRLAQQYSDTTLDCEYRKSMLSAAHVLAMDAKNLFDVVDSIRQRYQHLFPPSATKETSCSSSFESTSGSIVAEPVNDLGGYIKTSTSGDLLQNTGIYDNDLHHSFNSQLQLQNPKASIDLSGGGSLQRGMSLGLDTTRSTNEPLRIVEETLGSPGEHMYCNTSALHGHA

Target ORF information:

RefSeq Version	NM_166353.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster focal adhesion kinase, transcript variant C (Fak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166353.3

ORF Insert Sequence:

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fak ( NM_079069.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523793.2 Drosophila melanogaster focal adhesion kinase, transcript variant A (Fak), mRNA.

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CloneID	OFa10635
Clone ID Related Accession (Same CDS sequence)	NM_166352.3 , NM_166353.3 , NM_079069.4 , NM_001144247.3
Accession Version	NM_079069.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	focal adhesion kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079069.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523793.2
CDS	664..4266
Translation	MNTAGATSQPPPTKNEINSEEYLIHVHMPNKSFKAVRFNVKETVFHVIRRTVEDLGTDGRTPSIQRYACRMLNMITKEVIWLARSTSMQKVLSHILTPGCSNVDCPNNQSELDEVLLEHGRRITDNRVWRVELRVRYVPNNIQELFEEDKATCFYYFNQVKEDFIQANVTAIDTEVAVQLCCLGIRHYFKNITVKAPDKKQHIDYIEKEIGFKSFLPQSVIATSKPKNLKKLIQVGYKKVYNYNDIEYLTRFFDLLKNIYLTNFEQFSVTLSSAWNISGILHVGPHIGISYQTHPQASLKNVAQFKDVVSIKTCTLPKEKLSKSGENTTEPELQNFNCNCQKIKTQIKISASNNVEDLVITCNGINTAESIADLIDGYCRLLSKDLEFTIWHRETNASNEDSAKALPNDATLGSNKSTSSQGKPMLTDDYAEIGLLEGEGDYSTPTVRNYELDRALITPSAKIGVGQFGDVYVGTYTLPKLGKGKNLAGNGKNSNSDQRNADSRPDVIQVAIKTCKANDDPEKTENFLAEAYIMQKFDHPHIIRLIGICSVMPIWIVMELAKLGELRAYLKTNSERLSHGTLLKYCYQLSTALSYLESKKFVHRDIAARNVLVSSPTCVKLADFGLSRWVSDQSYYHSTPTVALPIKWMSPESINFRRFTTASDVWMFGVCIWEILMLGVKPFQGVKNSDVILKLENGERLPLPPNCPPRLYSLMSQCWAYEPLKRPNFKRIKETLHEILIEDSINSSETLKREQRKVASMSWIGSDDIDIPPSKPSRVMHDPDITGLMPETTGLPQTYIIAQNPAVLAKLMMENQKRGINPAAYTTPASGIHNVLGEKLRQQQKDSNSDSEWLIQEELLRQRSCSIPQGSLNDHQAQMFKLDFMSAGPSSLPDCSNSSSRPMTPNANLSSLKSNHSSADHLSSLTSAEEQMGSNARNLGSAVPSRPPNRADDEVYCATTLVVKSIMALSQGVEKANTEGYLELVKNVGVKLRNLLTSVDKISIIFPAQALKEVQMAHQVLSKDMHELVSAMRLAQQYSDTTLDCEYRKSMLSAAHVLAMDAKNLFDVVDSIRQRYQHLFPPSATKETSCSSSFESTSGSIVAEPVNDLGGYIKTSTSGDLLQNTGIYDNDLHHSFNSQLQLQNPKASIDLSGGGSLQRGMSLGLDTTRSTNEPLRIVEETLGSPGEHMYCNTSALHGHA

Target ORF information:

RefSeq Version	NM_079069.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster focal adhesion kinase, transcript variant A (Fak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079069.4

ORF Insert Sequence:

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fak ( NM_001144247.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137719.2 Drosophila melanogaster focal adhesion kinase, transcript variant G (Fak), mRNA.

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CloneID	OFa10635
Clone ID Related Accession (Same CDS sequence)	NM_166352.3 , NM_166353.3 , NM_079069.4 , NM_001144247.3
Accession Version	NM_001144247.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	focal adhesion kinase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144247.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137719.2
CDS	93..3695
Translation	MNTAGATSQPPPTKNEINSEEYLIHVHMPNKSFKAVRFNVKETVFHVIRRTVEDLGTDGRTPSIQRYACRMLNMITKEVIWLARSTSMQKVLSHILTPGCSNVDCPNNQSELDEVLLEHGRRITDNRVWRVELRVRYVPNNIQELFEEDKATCFYYFNQVKEDFIQANVTAIDTEVAVQLCCLGIRHYFKNITVKAPDKKQHIDYIEKEIGFKSFLPQSVIATSKPKNLKKLIQVGYKKVYNYNDIEYLTRFFDLLKNIYLTNFEQFSVTLSSAWNISGILHVGPHIGISYQTHPQASLKNVAQFKDVVSIKTCTLPKEKLSKSGENTTEPELQNFNCNCQKIKTQIKISASNNVEDLVITCNGINTAESIADLIDGYCRLLSKDLEFTIWHRETNASNEDSAKALPNDATLGSNKSTSSQGKPMLTDDYAEIGLLEGEGDYSTPTVRNYELDRALITPSAKIGVGQFGDVYVGTYTLPKLGKGKNLAGNGKNSNSDQRNADSRPDVIQVAIKTCKANDDPEKTENFLAEAYIMQKFDHPHIIRLIGICSVMPIWIVMELAKLGELRAYLKTNSERLSHGTLLKYCYQLSTALSYLESKKFVHRDIAARNVLVSSPTCVKLADFGLSRWVSDQSYYHSTPTVALPIKWMSPESINFRRFTTASDVWMFGVCIWEILMLGVKPFQGVKNSDVILKLENGERLPLPPNCPPRLYSLMSQCWAYEPLKRPNFKRIKETLHEILIEDSINSSETLKREQRKVASMSWIGSDDIDIPPSKPSRVMHDPDITGLMPETTGLPQTYIIAQNPAVLAKLMMENQKRGINPAAYTTPASGIHNVLGEKLRQQQKDSNSDSEWLIQEELLRQRSCSIPQGSLNDHQAQMFKLDFMSAGPSSLPDCSNSSSRPMTPNANLSSLKSNHSSADHLSSLTSAEEQMGSNARNLGSAVPSRPPNRADDEVYCATTLVVKSIMALSQGVEKANTEGYLELVKNVGVKLRNLLTSVDKISIIFPAQALKEVQMAHQVLSKDMHELVSAMRLAQQYSDTTLDCEYRKSMLSAAHVLAMDAKNLFDVVDSIRQRYQHLFPPSATKETSCSSSFESTSGSIVAEPVNDLGGYIKTSTSGDLLQNTGIYDNDLHHSFNSQLQLQNPKASIDLSGGGSLQRGMSLGLDTTRSTNEPLRIVEETLGSPGEHMYCNTSALHGHA

Target ORF information:

RefSeq Version	NM_001144247.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster focal adhesion kinase, transcript variant G (Fak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144247.3

ORF Insert Sequence:

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
CGGCAGAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fak ( NM_001169736.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163207.1 Drosophila melanogaster focal adhesion kinase, transcript variant F (Fak), mRNA.

Price & Availability↑

CloneID	OFa12137
Clone ID Related Accession (Same CDS sequence)	NM_001169736.2
Accession Version	NM_001169736.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	focal adhesion kinase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169736.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
AGATCCTGCT CAATACCTCA AGGATCGCTC AATGATCATC AGGCTCAAAT GTTTAAGCTT 
GACTTCATGT CAGCTGGTCC TTCCAGTTTG CCGGACTGCT CGAACTCCAG TTCTCGACCT 
ATGACACCAA ATGCCAATCT TTCTTCACTG AAGTCGAACC ACTCATCGGC GGATCATTTG 
TCCAGCTTGA CATCTGCAGA AGAACAGATG GGTTCAAATG CACGAAACCT GGGCAGTGCA 
GTTCCAAGTC GACCACCTAA CCGCGCAGAT GACGAAGTTT ATTGCGCCAC CACACTGGTG 
GTCAAATCAA TAATGGCGCT GTCACAAGGT GTGGAGAAAG CGAATACCGA GGGTTACTTG 
GAATTGGTTA AGAACGTGGG CGTCAAGTTG AGAAACTTGC TAACATCGGT GGACAAAATA 
TCTATAATAT TTCCAGCACA GGCCCTCAAG GAAGTGCAAA TGGCACATCA GGTACTTTCA 
AAAGACATGC ACGAATTGGT CTCAGCGATG CGATTGGCTC AACAATATAG TGACACAACG 
CTGGATTGTG AATATCGCAA GAGTATGCTG TCTGCTGCCC ACGTTTTGGC TATGGACGCC 
AAAAACCTGT TTGATGTTGT CGATTCGATA CGTCAACGTT ATCAGCATCT ATTCCCGCCA 
TCCGCCACAA AAGAAACAAG TTGTTCGTCA AGTTTCGAGT CGACTTCTGG ATCTATTGTC 
GCAGAGCCAG TTAATGACCT TGGTGGTTAT ATCAAGACTA GCACTTCTGG AGATTTGCTT 
CAAAACACAG GAATATATGA TAATGATTTG CATCATAGCT TCAACTCGCA ATTGCAGTTG 
CAAAACCCAA AAGCCAGCAT CGACTTAAGC GGCGGTGGTA GTCTACAGCG AGGGATGAGC 
CTTGGCTTGG ACACAACCAG GTCGACAAAC GAACCGTTGC GAATTGTTGA GGAGACCCTG 
GGCAGCCCGG GTGAACATAT GTACTGCAAT ACGTCCGCCT TGCACGGCCA CGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163207.1
CDS	664..4260
Translation	MNTAGATSQPPPTKNEINSEEYLIHVHMPNKSFKAVRFNVKETVFHVIRRTVEDLGTDGRTPSIQRYACRMLNMITKEVIWLARSTSMQKVLSHILTPGCSNVDCPNNQSELDEVLLEHGRRITDNRVWRVELRVRYVPNNIQELFEEDKATCFYYFNQVKEDFIQANVTAIDTEVAVQLCCLGIRHYFKNITVKAPDKKQHIDYIEKEIGFKSFLPQSVIATSKPKNLKKLIQVGYKKVYNYNDIEYLTRFFDLLKNIYLTNFEQFSVTLSSAWNISGILHVGPHIGISYQTHPQASLKNVAQFKDVVSIKTCTLPKEKLSKSGENTTEPELQNFNCNCQKIKTQIKISASNNVEDLVITCNGINTAESIADLIDGYCRLLSKDLEFTIWHRETNASNEDSAKALPNDATLGSNKSTSSQGKPMLTDDYAEIGLLEGEGDYSTPTVRNYELDRALITPSAKIGVGQFGDVYVGTYTLPKLGKGKNLAGNGKNSNSDQRNADSRPDVIQVAIKTCKANDDPEKTENFLAEAYIMQKFDHPHIIRLIGICSVMPIWIVMELAKLGELRAYLKTNSERLSHGTLLKYCYQLSTALSYLESKKFVHRDIAARNVLVSSPTCVKLADFGLSRWVSDQSYYHSTPTVALPIKWMSPESINFRRFTTASDVWMFGVCIWEILMLGVKPFQGVKNSDVILKLENGERLPLPPNCPPRLYSLMSQCWAYEPLKRPNFKRIKETLHEILIEDSINSSETLKREQRKVASMSWIGSDDIDIPPSKPSRVMHDPDITGLMPETTGLPQTYIIAQNPAVLAKLMMENQKRGINPAAYTTPASGIHNVLGEKLRQQQKDSNSDSEWLIQEELLRSCSIPQGSLNDHQAQMFKLDFMSAGPSSLPDCSNSSSRPMTPNANLSSLKSNHSSADHLSSLTSAEEQMGSNARNLGSAVPSRPPNRADDEVYCATTLVVKSIMALSQGVEKANTEGYLELVKNVGVKLRNLLTSVDKISIIFPAQALKEVQMAHQVLSKDMHELVSAMRLAQQYSDTTLDCEYRKSMLSAAHVLAMDAKNLFDVVDSIRQRYQHLFPPSATKETSCSSSFESTSGSIVAEPVNDLGGYIKTSTSGDLLQNTGIYDNDLHHSFNSQLQLQNPKASIDLSGGGSLQRGMSLGLDTTRSTNEPLRIVEETLGSPGEHMYCNTSALHGHA

Target ORF information:

RefSeq Version	NM_001169736.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster focal adhesion kinase, transcript variant F (Fak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169736.2

ORF Insert Sequence:

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GGCATTCACA ATGTTTTGGG CGAAAAACTA 
CGACAACAGC AAAAGGATAG CAACAGCGAC AGCGAATGGT TAATTCAAGA AGAATTGCTA 
AGATCCTGCT CAATACCTCA AGGATCGCTC AATGATCATC AGGCTCAAAT GTTTAAGCTT 
GACTTCATGT CAGCTGGTCC TTCCAGTTTG CCGGACTGCT CGAACTCCAG TTCTCGACCT 
ATGACACCAA ATGCCAATCT TTCTTCACTG AAGTCGAACC ACTCATCGGC GGATCATTTG 
TCCAGCTTGA CATCTGCAGA AGAACAGATG GGTTCAAATG CACGAAACCT GGGCAGTGCA 
GTTCCAAGTC GACCACCTAA CCGCGCAGAT GACGAAGTTT ATTGCGCCAC CACACTGGTG 
GTCAAATCAA TAATGGCGCT GTCACAAGGT GTGGAGAAAG CGAATACCGA GGGTTACTTG 
GAATTGGTTA AGAACGTGGG CGTCAAGTTG AGAAACTTGC TAACATCGGT GGACAAAATA 
TCTATAATAT TTCCAGCACA GGCCCTCAAG GAAGTGCAAA TGGCACATCA GGTACTTTCA 
AAAGACATGC ACGAATTGGT CTCAGCGATG CGATTGGCTC AACAATATAG TGACACAACG 
CTGGATTGTG AATATCGCAA GAGTATGCTG TCTGCTGCCC ACGTTTTGGC TATGGACGCC 
AAAAACCTGT TTGATGTTGT CGATTCGATA CGTCAACGTT ATCAGCATCT ATTCCCGCCA 
TCCGCCACAA AAGAAACAAG TTGTTCGTCA AGTTTCGAGT CGACTTCTGG ATCTATTGTC 
GCAGAGCCAG TTAATGACCT TGGTGGTTAT ATCAAGACTA GCACTTCTGG AGATTTGCTT 
CAAAACACAG GAATATATGA TAATGATTTG CATCATAGCT TCAACTCGCA ATTGCAGTTG 
CAAAACCCAA AAGCCAGCAT CGACTTAAGC GGCGGTGGTA GTCTACAGCG AGGGATGAGC 
CTTGGCTTGG ACACAACCAG GTCGACAAAC GAACCGTTGC GAATTGTTGA GGAGACCCTG 
GGCAGCCCGG GTGAACATAT GTACTGCAAT ACGTCCGCCT TGCACGGCCA CGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fak ( NM_001144246.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137718.1 Drosophila melanogaster focal adhesion kinase, transcript variant D (Fak), mRNA.

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CloneID	OFa10636
Clone ID Related Accession (Same CDS sequence)	NM_001144246.3
Accession Version	NM_001144246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	focal adhesion kinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144246.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GTATTTAATA CTCTAGCCGT AGGATTAGAT 
GACAGCAATC CGAATGTTTC GCTTAAGACT ACTAAGCTAC CAGCAGCAGA TTTGTTAAAA 
CTTGACCCCA TCGCAGAATC CGAAAGGCTT AGGTCCCAAT TTTCAGCTAA TAGCAGCGCT 
AGTCAACTGG CTAACCCCCT TACTGCTTCA GTTAACCCCC ATGAATCCCT CAATTCACAC 
CAGGCAGGAA TGTTTACGGG TAGTTTGCCT TCAAAGAGCA GCTTCGTCGT TTGCCCACCC 
CAAACAGAGG AACACATGCA AGCCGAAAAC ATTTCTAACC CGTCTGTGTC CAAGGTGTCC 
GGATATAGCC TATATGGGAG CTTGGAGAGG CACCAACCAC CACCAGCAAA AGCCATGCAC 
TCCAAAGGTG GCAGCCTGGA ACGCCACCAG TCATTGATGT CAGCACAAAA TCTTGTTTTC 
TACAATACAA AGCCGCCACC AAACTGCACG AATACCGCAG AGCGATCGAG GAGCATGGAG 
CGGAACACAT ACTTCCATGC CTATCGCCAA CAGATGAAAA CCTCTATTGA ATGCGAAGCT 
CTACCCGAGG AGATTTACGA TTTTGGGGGC ATCGGACTCA AGACCTGTGT GTCCGCTAGG 
CAGCAACCCA AGTTTAGCCC AATGGTTAAT GTGCGCCCAG CTGAACTGGG CCAAGTACAA 
AACACAGATT GCATGGGATT ACAGCAGAAT ATTCCAATGG ACTCCTTTGG AGTTATATCC 
CCACACAACT TGGACGAAAG TCTAAGACAT CAGAGAGAAA TGGAGCGACA GTGGATGACT 
TCGGGACCAC AGAGCATCAC AGTTAACCCA AACATTTCCA TTTCCGAAGG AGCTGCTTGC 
TTGGCCACAT TGCAAGCTGA AGCAATGGGA AATCAGGGCA TTCACAATGT TTTGGGCGAA 
AAACTACGAC AACAGCAAAA GGATAGCAAC AGCGACAGCG AATGGTTAAT TCAAGAAGAA 
TTGCTAAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137718.1
CDS	664..5166
Translation	MNTAGATSQPPPTKNEINSEEYLIHVHMPNKSFKAVRFNVKETVFHVIRRTVEDLGTDGRTPSIQRYACRMLNMITKEVIWLARSTSMQKVLSHILTPGCSNVDCPNNQSELDEVLLEHGRRITDNRVWRVELRVRYVPNNIQELFEEDKATCFYYFNQVKEDFIQANVTAIDTEVAVQLCCLGIRHYFKNITVKAPDKKQHIDYIEKEIGFKSFLPQSVIATSKPKNLKKLIQVGYKKVYNYNDIEYLTRFFDLLKNIYLTNFEQFSVTLSSAWNISGILHVGPHIGISYQTHPQASLKNVAQFKDVVSIKTCTLPKEKLSKSGENTTEPELQNFNCNCQKIKTQIKISASNNVEDLVITCNGINTAESIADLIDGYCRLLSKDLEFTIWHRETNASNEDSAKALPNDATLGSNKSTSSQGKPMLTDDYAEIGLLEGEGDYSTPTVRNYELDRALITPSAKIGVGQFGDVYVGTYTLPKLGKGKNLAGNGKNSNSDQRNADSRPDVIQVAIKTCKANDDPEKTENFLAEAYIMQKFDHPHIIRLIGICSVMPIWIVMELAKLGELRAYLKTNSERLSHGTLLKYCYQLSTALSYLESKKFVHRDIAARNVLVSSPTCVKLADFGLSRWVSDQSYYHSTPTVALPIKWMSPESINFRRFTTASDVWMFGVCIWEILMLGVKPFQGVKNSDVILKLENGERLPLPPNCPPRLYSLMSQCWAYEPLKRPNFKRIKETLHEILIEDSINSSETLKREQRKVASMSWIGSDDIDIPPSKPSRVMHDPDITGLMPETTGLPQTYIIAQNPAVLAKLMMENQKRGINPAAYTTPASVFNTLAVGLDDSNPNVSLKTTKLPAADLLKLDPIAESERLRSQFSANSSASQLANPLTASVNPHESLNSHQAGMFTGSLPSKSSFVVCPPQTEEHMQAENISNPSVSKVSGYSLYGSLERHQPPPAKAMHSKGGSLERHQSLMSAQNLVFYNTKPPPNCTNTAERSRSMERNTYFHAYRQQMKTSIECEALPEEIYDFGGIGLKTCVSARQQPKFSPMVNVRPAELGQVQNTDCMGLQQNIPMDSFGVISPHNLDESLRHQREMERQWMTSGPQSITVNPNISISEGAACLATLQAEAMGNQGIHNVLGEKLRQQQKDSNSDSEWLIQEELLRSCSIPQGSLNDHQAQMFKLDFMSAGPSSLPDCSNSSSRPMTPNANLSSLKSNHSSADHLSSLTSAEEQMGSNARNLGSAVPSRPPNRADDEVYCATTLVVKSIMALSQGVEKANTEGYLELVKNVGVKLRNLLTSVDKISIIFPAQALKEVQMAHQVLSKDMHELVSAMRLAQQYSDTTLDCEYRKSMLSAAHVLAMDAKNLFDVVDSIRQRYQHLFPPSATKETSCSSSFESTSGSIVAEPVNDLGGYIKTSTSGDLLQNTGIYDNDLHHSFNSQLQLQNPKASIDLSGGGSLQRGMSLGLDTTRSTNEPLRIVEETLGSPGEHMYCNTSALHGHA

Target ORF information:

RefSeq Version	NM_001144246.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster focal adhesion kinase, transcript variant D (Fak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144246.3

ORF Insert Sequence:

ATGAACACCG CGGGAGCCAC CAGTCAACCG CCGCCCACTA AAAATGAGAT TAACTCCGAG 
GAGTATCTCA TCCACGTGCA TATGCCGAAC AAGAGCTTCA AGGCTGTTCG GTTTAATGTC 
AAGGAGACCG TTTTCCATGT GATCCGGCGC ACTGTCGAGG ATCTGGGCAC GGATGGACGG 
ACGCCCAGCA TTCAGCGATA TGCCTGCCGC ATGCTTAACA TGATCACCAA GGAGGTGATT 
TGGCTGGCTA GAAGCACTTC AATGCAGAAG GTTCTCTCGC ACATCCTGAC GCCCGGCTGC 
TCCAACGTTG ACTGTCCCAA CAACCAGTCG GAGTTGGATG AGGTTCTATT GGAGCACGGA 
AGAAGGATCA CCGATAATAG GGTGTGGCGA GTGGAGCTCA GAGTGCGCTA CGTGCCAAAT 
AATATTCAAG AGCTCTTCGA GGAGGACAAG GCCACATGCT TCTATTATTT CAATCAGGTG 
AAAGAGGACT TTATCCAAGC CAATGTCACA GCCATCGACA CTGAAGTGGC GGTGCAACTG 
TGCTGTCTGG GCATTCGTCA TTATTTCAAG AACATCACCG TGAAAGCACC TGACAAAAAG 
CAGCACATTG ACTACATTGA AAAGGAAATC GGATTTAAAA GTTTTCTTCC ACAATCTGTG 
ATAGCCACAT CAAAGCCAAA GAATCTTAAG AAACTGATCC AAGTCGGTTA CAAAAAGGTC 
TACAATTACA ACGACATTGA GTACTTGACG CGGTTCTTTG ATCTTCTGAA GAATATTTAT 
TTAACGAACT TCGAGCAGTT CTCGGTAACC CTGAGCTCGG CGTGGAATAT TTCTGGAATT 
CTACACGTCG GCCCTCACAT TGGAATCTCG TACCAGACTC ATCCTCAGGC CAGCTTGAAG 
AACGTGGCTC AGTTTAAAGA TGTGGTCTCT ATTAAAACGT GCACTTTACC AAAGGAAAAA 
CTGTCCAAGT CTGGGGAGAA TACCACAGAA CCAGAGCTTC AGAATTTTAA TTGCAACTGC 
CAGAAGATTA AAACCCAAAT AAAAATATCC GCTTCCAACA ATGTGGAAGA TTTGGTTATA 
ACGTGCAATG GTATTAATAC CGCTGAGAGT ATTGCTGACC TAATTGACGG TTACTGCCGG 
CTGTTATCAA AAGACCTAGA GTTCACGATT TGGCATCGAG AGACAAACGC GTCGAACGAA 
GATAGCGCAA AAGCATTGCC CAATGATGCG ACGCTGGGGT CCAATAAATC AACTTCAAGT 
CAGGGAAAAC CGATGCTGAC CGATGATTAT GCCGAGATTG GTTTATTGGA GGGCGAGGGC 
GACTACTCTA CGCCCACCGT TCGAAATTAT GAGTTGGACA GAGCCCTCAT AACGCCGAGC 
GCCAAAATTG GTGTGGGACA GTTTGGTGAT GTGTATGTAG GCACGTATAC GCTTCCGAAA 
CTGGGCAAGG GCAAGAACTT AGCAGGAAAT GGAAAAAATA GTAATAGTGA CCAAAGAAAT 
GCCGATTCAA GGCCAGATGT TATACAAGTG GCGATAAAGA CATGTAAAGC TAACGACGAT 
CCTGAAAAAA CCGAAAATTT TCTTGCCGAA GCTTATATTA TGCAAAAATT CGATCATCCC 
CATATTATAC GCTTAATCGG CATTTGCAGC GTAATGCCCA TTTGGATAGT TATGGAATTG 
GCCAAACTGG GTGAATTGCG TGCGTACTTA AAGACAAACA GCGAAAGATT AAGCCACGGT 
ACTTTACTGA AGTATTGCTA TCAGCTATCG ACTGCTCTTA GTTATTTGGA ATCCAAAAAG 
TTTGTTCACC GAGATATAGC GGCGCGTAAT GTACTAGTCA GCTCACCAAC GTGTGTTAAG 
TTGGCTGATT TTGGATTATC ACGTTGGGTT TCCGATCAGT CGTATTATCA CTCAACACCC 
ACAGTTGCCC TACCCATTAA ATGGATGTCC CCCGAGTCAA TAAACTTTAG AAGATTTACC 
ACTGCTAGTG ATGTTTGGAT GTTTGGTGTC TGCATTTGGG AAATACTCAT GCTCGGTGTA 
AAGCCTTTCC AAGGCGTCAA GAACAGCGAT GTTATATTGA AGCTCGAAAA CGGAGAGCGT 
CTGCCATTGC CTCCCAACTG CCCACCTAGG TTATATTCGT TAATGTCCCA ATGCTGGGCG 
TACGAGCCAC TTAAACGACC GAATTTCAAG CGGATCAAGG AAACTCTGCA TGAAATTCTG 
ATTGAAGACA GCATTAATTC ATCGGAGACA CTGAAGCGGG AGCAACGAAA AGTGGCTTCC 
ATGTCCTGGA TTGGCAGTGA TGACATCGAC ATTCCGCCAT CGAAACCTTC AAGGGTGATG 
CACGATCCTG ACATCACTGG CTTAATGCCT GAAACAACGG GGCTACCTCA GACCTATATT 
ATTGCACAAA ATCCCGCGGT GCTGGCCAAA CTGATGATGG AGAACCAAAA ACGAGGCATA 
AATCCAGCGG CGTACACCAC ACCAGCTTCG GTATTTAATA CTCTAGCCGT AGGATTAGAT 
GACAGCAATC CGAATGTTTC GCTTAAGACT ACTAAGCTAC CAGCAGCAGA TTTGTTAAAA 
CTTGACCCCA TCGCAGAATC CGAAAGGCTT AGGTCCCAAT TTTCAGCTAA TAGCAGCGCT 
AGTCAACTGG CTAACCCCCT TACTGCTTCA GTTAACCCCC ATGAATCCCT CAATTCACAC 
CAGGCAGGAA TGTTTACGGG TAGTTTGCCT TCAAAGAGCA GCTTCGTCGT TTGCCCACCC 
CAAACAGAGG AACACATGCA AGCCGAAAAC ATTTCTAACC CGTCTGTGTC CAAGGTGTCC 
GGATATAGCC TATATGGGAG CTTGGAGAGG CACCAACCAC CACCAGCAAA AGCCATGCAC 
TCCAAAGGTG GCAGCCTGGA ACGCCACCAG TCATTGATGT CAGCACAAAA TCTTGTTTTC 
TACAATACAA AGCCGCCACC AAACTGCACG AATACCGCAG AGCGATCGAG GAGCATGGAG 
CGGAACACAT ACTTCCATGC CTATCGCCAA CAGATGAAAA CCTCTATTGA ATGCGAAGCT 
CTACCCGAGG AGATTTACGA TTTTGGGGGC ATCGGACTCA AGACCTGTGT GTCCGCTAGG 
CAGCAACCCA AGTTTAGCCC AATGGTTAAT GTGCGCCCAG CTGAACTGGG CCAAGTACAA 
AACACAGATT GCATGGGATT ACAGCAGAAT ATTCCAATGG ACTCCTTTGG AGTTATATCC 
CCACACAACT TGGACGAAAG TCTAAGACAT CAGAGAGAAA TGGAGCGACA GTGGATGACT 
TCGGGACCAC AGAGCATCAC AGTTAACCCA AACATTTCCA TTTCCGAAGG AGCTGCTTGC 
TTGGCCACAT TGCAAGCTGA AGCAATGGGA AATCAGGGCA TTCACAATGT TTTGGGCGAA 
AAACTACGAC AACAGCAAAA GGATAGCAAC AGCGACAGCG AATGGTTAAT TCAAGAAGAA 
TTGCTAAGAT CCTGCTCAAT ACCTCAAGGA TCGCTCAATG ATCATCAGGC TCAAATGTTT 
AAGCTTGACT TCATGTCAGC TGGTCCTTCC AGTTTGCCGG ACTGCTCGAA CTCCAGTTCT 
CGACCTATGA CACCAAATGC CAATCTTTCT TCACTGAAGT CGAACCACTC ATCGGCGGAT 
CATTTGTCCA GCTTGACATC TGCAGAAGAA CAGATGGGTT CAAATGCACG AAACCTGGGC 
AGTGCAGTTC CAAGTCGACC ACCTAACCGC GCAGATGACG AAGTTTATTG CGCCACCACA 
CTGGTGGTCA AATCAATAAT GGCGCTGTCA CAAGGTGTGG AGAAAGCGAA TACCGAGGGT 
TACTTGGAAT TGGTTAAGAA CGTGGGCGTC AAGTTGAGAA ACTTGCTAAC ATCGGTGGAC 
AAAATATCTA TAATATTTCC AGCACAGGCC CTCAAGGAAG TGCAAATGGC ACATCAGGTA 
CTTTCAAAAG ACATGCACGA ATTGGTCTCA GCGATGCGAT TGGCTCAACA ATATAGTGAC 
ACAACGCTGG ATTGTGAATA TCGCAAGAGT ATGCTGTCTG CTGCCCACGT TTTGGCTATG 
GACGCCAAAA ACCTGTTTGA TGTTGTCGAT TCGATACGTC AACGTTATCA GCATCTATTC 
CCGCCATCCG CCACAAAAGA AACAAGTTGT TCGTCAAGTT TCGAGTCGAC TTCTGGATCT 
ATTGTCGCAG AGCCAGTTAA TGACCTTGGT GGTTATATCA AGACTAGCAC TTCTGGAGAT 
TTGCTTCAAA ACACAGGAAT ATATGATAAT GATTTGCATC ATAGCTTCAA CTCGCAATTG 
CAGTTGCAAA ACCCAAAAGC CAGCATCGAC TTAAGCGGCG GTGGTAGTCT ACAGCGAGGG 
ATGAGCCTTG GCTTGGACAC AACCAGGTCG ACAAACGAAC CGTTGCGAAT TGTTGAGGAG 
ACCCTGGGCA GCCCGGGTGA ACATATGTAC TGCAATACGT CCGCCTTGCA CGGCCACGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fak cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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