EndoB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	EndoB
Entrez Gene ID	37218
Full Name	Endophilin B
Synonyms	CG9834,D-EndoB,D-endoB,Dmel\CG9834,endoB
General protein information	Preferred Names Endophilin B Names CG9834 gene product from transcript CG9834-RB CG9834-PA CG9834-PB CG9834-PC CG9834-PD EndoB-PA EndoB-PB EndoB-PC EndoB-PD endophilin endophilin B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56C10-56C10

mRNA and Protein(s)

mRNA	Protein	Name
NM_166340.3	NP_725873.1	endophilin B, isoform B
NM_001103905.3	NP_001097375.1	endophilin B, isoform C
NM_001103906.3	NP_001097376.1	endophilin B, isoform D
NM_137566.4	NP_611410.1	endophilin B, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sh3glb1a	NP_001017617.1
Bos taurus (cattle)	SH3GLB1	NP_001071461.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010376	XP_311569.4
Homo sapiens (human)	SH3GLB1	NP_001193581.1
Gallus gallus (chicken)	SH3GLB1	NP_001006534.1
Xenopus tropicalis (tropical clawed frog)	sh3glb1	NP_001016072.1
	SH3GLB1	XP_001109732.1
Canis lupus familiaris (dog)	SH3GLB1	XP_005622022.1
Mus musculus (house mouse)	Sh3glb1	NP_001268966.1
Rattus norvegicus (Norway rat)	Sh3glb1	NP_001011929.1
Drosophila melanogaster (fruit fly)	endoB	NP_611410.1
Pan troglodytes (chimpanzee)	SH3GLB1	XP_003308231.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results allow us to conclude that D-EndoB contributes to the endocytic activity downstream of Oskar by facilitating membrane dynamics through its N-BAR domain.
Title: Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar.

The following EndoB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EndoB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10613	NM_166340.3 Latest version!	Drosophila melanogaster endophilin B, transcript variant B (EndoB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10614	NM_001103906.3 Latest version!	Drosophila melanogaster endophilin B, transcript variant D (EndoB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10615	NM_137566.4 Latest version!	Drosophila melanogaster endophilin B, transcript variant A (EndoB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10616	NM_001103905.3 Latest version!	Drosophila melanogaster endophilin B, transcript variant C (EndoB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EndoB ( NM_166340.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725873.1 Drosophila melanogaster endophilin B, transcript variant B (EndoB), mRNA.

Price & Availability↑

CloneID	OFa10613
Clone ID Related Accession (Same CDS sequence)	NM_166340.3
Accession Version	NM_166340.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endophilin B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166340.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGGCGG AACGGGACTT GCGGGTGGCT 
CAGGCCGAGT TCGACCGGCA GGCGGAGATT ACCAAGCTGC TGCTGGACGG GATCAGCACA 
TCGCAGGCAT CTCATTTGCG CCACTTGCAC GCCTTCATCC AGACGCAAGT GCGCTACTAC 
AAGCAGTGCG GCGATGTCAT GGAGCAGCTG CAGCGTGAAC TGGCCAACTT GGGAGGCCCC 
ACGCCATACA TTCCGCTCGA CGTGAACGAG GCCAGTGCCA GCAAGTCCAA CATATCGTCC 
GGAGCCGCAG CCCGTGGTCC TGGCAACAAT CACTCTGCCA ATATGGCTGC CACCGGCCAC 
AAGCCTAATC AGCCCATGCA CGTCAGCACG GATCAGATGC AGAGGGCACG CGTCCTTTGC 
TCCTACGACG CCAAGGATCA CACCGAGCTT AATTTGAGCG CCAATGAGGT GATCTTCGTG 
ACGGAGTGCT CACCAGTAAA CGAGGATTAC ATGTACGGAA AACAGGGTCT ATTGAAGGGT 
CTGGTGCCAC GCGCATTTGT TGAAATGCTC GACGAAGAGC ACGATGTCAC CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725873.1
CDS	114..1250
Translation	MNINLPNFNVKNLVKEAGSTISRVVQLTEEKLGTTERTEYDLHFQNLAERADVTKTWTEKIVRDTESVLIPNPQNRVEDFIFEKIEKSKPKRLSNLEHLALDMIEAGGDFGQDLPYGQALIKVGQAEQKLGQCEHDFIATSGICFTQPLRKFLDGEMKTIGKERGILETKRLDLDACKNRVKKARSMLGQQSAERDLRVAQAEFDRQAEITKLLLDGISTSQASHLRHLHAFIQTQVRYYKQCGDVMEQLQRELANLGGPTPYIPLDVNEASASKSNISSGAAARGPGNNHSANMAATGHKPNQPMHVSTDQMQRARVLCSYDAKDHTELNLSANEVIFVTECSPVNEDYMYGKQGLLKGLVPRAFVEMLDEEHDVTL

Target ORF information:

RefSeq Version	NM_166340.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endophilin B, transcript variant B (EndoB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166340.3

ORF Insert Sequence:

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGGCGG AACGGGACTT GCGGGTGGCT 
CAGGCCGAGT TCGACCGGCA GGCGGAGATT ACCAAGCTGC TGCTGGACGG GATCAGCACA 
TCGCAGGCAT CTCATTTGCG CCACTTGCAC GCCTTCATCC AGACGCAAGT GCGCTACTAC 
AAGCAGTGCG GCGATGTCAT GGAGCAGCTG CAGCGTGAAC TGGCCAACTT GGGAGGCCCC 
ACGCCATACA TTCCGCTCGA CGTGAACGAG GCCAGTGCCA GCAAGTCCAA CATATCGTCC 
GGAGCCGCAG CCCGTGGTCC TGGCAACAAT CACTCTGCCA ATATGGCTGC CACCGGCCAC 
AAGCCTAATC AGCCCATGCA CGTCAGCACG GATCAGATGC AGAGGGCACG CGTCCTTTGC 
TCCTACGACG CCAAGGATCA CACCGAGCTT AATTTGAGCG CCAATGAGGT GATCTTCGTG 
ACGGAGTGCT CACCAGTAAA CGAGGATTAC ATGTACGGAA AACAGGGTCT ATTGAAGGGT 
CTGGTGCCAC GCGCATTTGT TGAAATGCTC GACGAAGAGC ACGATGTCAC CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EndoB ( NM_001103906.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097376.1 Drosophila melanogaster endophilin B, transcript variant D (EndoB), mRNA.

Price & Availability↑

CloneID	OFa10614
Clone ID Related Accession (Same CDS sequence)	NM_001103906.3
Accession Version	NM_001103906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endophilin B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103906.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGGCGG AACGGGACTT GCGGGTGGCT 
CAGGCCGAGT TCGACCGGCA GGCGGAGATT ACCAAGCTGC TGCTGGACGG GATCAGCACA 
TCGCAGGCAT CTCATTTGCG CCACTTGCAC GCCTTCATCC AGACGCAAGT GCGCTACTAC 
AAGCAGTGCG GCGATGTCAT GGAGCAGCTG CAGCGTGAAC TGGCCAAAAT GCAGCATCCC 
AAGCCACGCC TGCGTATCAA TAGCGAAGAC GTTGATTGTG GGCCTCCCAG CTCGGTGTCG 
ATGCATTCTT GTGATTCCGA TTCATTAGGG GGGATAGCCC TAGATAGCGA TCCAGATCCC 
GATTTGGACA AGTCGCTTAC GAATCTGCTC GAAGACTTTC ACATAGAATT CGATACGACA 
GCCGTCTCCA CAGTGATCTT CGTGACGGAG TGCTCACCAG TAAACGAGGA TTACATGTAC 
GGAAAACAGG GTCTATTGAA GGGTCTGGTG CCACGCGCAT TTGTTGAAAT GCTCGACGAA 
GAGCACGATG TCACCCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097376.1
CDS	114..1214
Translation	MNINLPNFNVKNLVKEAGSTISRVVQLTEEKLGTTERTEYDLHFQNLAERADVTKTWTEKIVRDTESVLIPNPQNRVEDFIFEKIEKSKPKRLSNLEHLALDMIEAGGDFGQDLPYGQALIKVGQAEQKLGQCEHDFIATSGICFTQPLRKFLDGEMKTIGKERGILETKRLDLDACKNRVKKARSMLGQQSAERDLRVAQAEFDRQAEITKLLLDGISTSQASHLRHLHAFIQTQVRYYKQCGDVMEQLQRELAKMQHPKPRLRINSEDVDCGPPSSVSMHSCDSDSLGGIALDSDPDPDLDKSLTNLLEDFHIEFDTTAVSTVIFVTECSPVNEDYMYGKQGLLKGLVPRAFVEMLDEEHDVTL

Target ORF information:

RefSeq Version	NM_001103906.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endophilin B, transcript variant D (EndoB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103906.3

ORF Insert Sequence:

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGGCGG AACGGGACTT GCGGGTGGCT 
CAGGCCGAGT TCGACCGGCA GGCGGAGATT ACCAAGCTGC TGCTGGACGG GATCAGCACA 
TCGCAGGCAT CTCATTTGCG CCACTTGCAC GCCTTCATCC AGACGCAAGT GCGCTACTAC 
AAGCAGTGCG GCGATGTCAT GGAGCAGCTG CAGCGTGAAC TGGCCAAAAT GCAGCATCCC 
AAGCCACGCC TGCGTATCAA TAGCGAAGAC GTTGATTGTG GGCCTCCCAG CTCGGTGTCG 
ATGCATTCTT GTGATTCCGA TTCATTAGGG GGGATAGCCC TAGATAGCGA TCCAGATCCC 
GATTTGGACA AGTCGCTTAC GAATCTGCTC GAAGACTTTC ACATAGAATT CGATACGACA 
GCCGTCTCCA CAGTGATCTT CGTGACGGAG TGCTCACCAG TAAACGAGGA TTACATGTAC 
GGAAAACAGG GTCTATTGAA GGGTCTGGTG CCACGCGCAT TTGTTGAAAT GCTCGACGAA 
GAGCACGATG TCACCCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EndoB ( NM_137566.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611410.1 Drosophila melanogaster endophilin B, transcript variant A (EndoB), mRNA.

Price & Availability↑

CloneID	OFa10615
Clone ID Related Accession (Same CDS sequence)	NM_137566.4
Accession Version	NM_137566.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endophilin B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137566.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGAAAG ATGGCATCTC GCCAGAGGCC 
GTCTTGGAAC AGGCGGAACG GGACTTGCGG GTGGCTCAGG CCGAGTTCGA CCGGCAGGCG 
GAGATTACCA AGCTGCTGCT GGACGGGATC AGCACATCGC AGGCATCTCA TTTGCGCCAC 
TTGCACGCCT TCATCCAGAC GCAAGTGCGC TACTACAAGC AGTGCGGCGA TGTCATGGAG 
CAGCTGCAGC GTGAACTGGC CAACTTGGGA GGCCCCACGC CATACATTCC GCTCGACGTG 
AACGAGGCCA GTGCCAGCAA GTCCAACATA TCGTCCGGAG CCGCAGCCCG TGGTCCTGGC 
AACAATCACT CTGCCAATAT GGCTGCCACC GGCCACAAGC CTAATCAGCC CATGCACGTC 
AGCACGGATC AGATGCAGAG GGCACGCGTC CTTTGCTCCT ACGACGCCAA GGATCACACC 
GAGCTTAATT TGAGCGCCAA TGAGGTGATC TTCGTGACGG AGTGCTCACC AGTAAACGAG 
GATTACATGT ACGGAAAACA GGGTCTATTG AAGGGTCTGG TGCCACGCGC ATTTGTTGAA 
ATGCTCGACG AAGAGCACGA TGTCACCCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611410.1
CDS	114..1286
Translation	MNINLPNFNVKNLVKEAGSTISRVVQLTEEKLGTTERTEYDLHFQNLAERADVTKTWTEKIVRDTESVLIPNPQNRVEDFIFEKIEKSKPKRLSNLEHLALDMIEAGGDFGQDLPYGQALIKVGQAEQKLGQCEHDFIATSGICFTQPLRKFLDGEMKTIGKERGILETKRLDLDACKNRVKKARSMLGQQSKDGISPEAVLEQAERDLRVAQAEFDRQAEITKLLLDGISTSQASHLRHLHAFIQTQVRYYKQCGDVMEQLQRELANLGGPTPYIPLDVNEASASKSNISSGAAARGPGNNHSANMAATGHKPNQPMHVSTDQMQRARVLCSYDAKDHTELNLSANEVIFVTECSPVNEDYMYGKQGLLKGLVPRAFVEMLDEEHDVTL

Target ORF information:

RefSeq Version	NM_137566.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endophilin B, transcript variant A (EndoB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137566.4

ORF Insert Sequence:

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGAAAG ATGGCATCTC GCCAGAGGCC 
GTCTTGGAAC AGGCGGAACG GGACTTGCGG GTGGCTCAGG CCGAGTTCGA CCGGCAGGCG 
GAGATTACCA AGCTGCTGCT GGACGGGATC AGCACATCGC AGGCATCTCA TTTGCGCCAC 
TTGCACGCCT TCATCCAGAC GCAAGTGCGC TACTACAAGC AGTGCGGCGA TGTCATGGAG 
CAGCTGCAGC GTGAACTGGC CAACTTGGGA GGCCCCACGC CATACATTCC GCTCGACGTG 
AACGAGGCCA GTGCCAGCAA GTCCAACATA TCGTCCGGAG CCGCAGCCCG TGGTCCTGGC 
AACAATCACT CTGCCAATAT GGCTGCCACC GGCCACAAGC CTAATCAGCC CATGCACGTC 
AGCACGGATC AGATGCAGAG GGCACGCGTC CTTTGCTCCT ACGACGCCAA GGATCACACC 
GAGCTTAATT TGAGCGCCAA TGAGGTGATC TTCGTGACGG AGTGCTCACC AGTAAACGAG 
GATTACATGT ACGGAAAACA GGGTCTATTG AAGGGTCTGG TGCCACGCGC ATTTGTTGAA 
ATGCTCGACG AAGAGCACGA TGTCACCCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EndoB ( NM_001103905.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097375.1 Drosophila melanogaster endophilin B, transcript variant C (EndoB), mRNA.

Price & Availability↑

CloneID	OFa10616
Clone ID Related Accession (Same CDS sequence)	NM_001103905.3
Accession Version	NM_001103905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	endophilin B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103905.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGAAAG ATGGCATCTC GCCAGAGGCC 
GTCTTGGAAC AGGCGGAACG GGACTTGCGG GTGGCTCAGG CCGAGTTCGA CCGGCAGGCG 
GAGATTACCA AGCTGCTGCT GGACGGGATC AGCACATCGC AGGCATCTCA TTTGCGCCAC 
TTGCACGCCT TCATCCAGAC GCAAGTGCGC TACTACAAGC AGTGCGGCGA TGTCATGGAG 
CAGCTGCAGC GTGAACTGGC CAAAATGCAG CATCCCAAGC CACGCCTGCG TATCAATAGC 
GAAGACGTTG ATTGTGGGCC TCCCAGCTCG GTGTCGATGC ATTCTTGTGA TTCCGATTCA 
TTAGGGGGGA TAGCCCTAGA TAGCGATCCA GATCCCGATT TGGACAAGTC GCTTACGAAT 
CTGCTCGAAG ACTTTCACAT AGAATTCGAT ACGACAGCCG TCTCCACAGT GATCTTCGTG 
ACGGAGTGCT CACCAGTAAA CGAGGATTAC ATGTACGGAA AACAGGGTCT ATTGAAGGGT 
CTGGTGCCAC GCGCATTTGT TGAAATGCTC GACGAAGAGC ACGATGTCAC CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097375.1
CDS	114..1250
Translation	MNINLPNFNVKNLVKEAGSTISRVVQLTEEKLGTTERTEYDLHFQNLAERADVTKTWTEKIVRDTESVLIPNPQNRVEDFIFEKIEKSKPKRLSNLEHLALDMIEAGGDFGQDLPYGQALIKVGQAEQKLGQCEHDFIATSGICFTQPLRKFLDGEMKTIGKERGILETKRLDLDACKNRVKKARSMLGQQSKDGISPEAVLEQAERDLRVAQAEFDRQAEITKLLLDGISTSQASHLRHLHAFIQTQVRYYKQCGDVMEQLQRELAKMQHPKPRLRINSEDVDCGPPSSVSMHSCDSDSLGGIALDSDPDPDLDKSLTNLLEDFHIEFDTTAVSTVIFVTECSPVNEDYMYGKQGLLKGLVPRAFVEMLDEEHDVTL

Target ORF information:

RefSeq Version	NM_001103905.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster endophilin B, transcript variant C (EndoB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103905.3

ORF Insert Sequence:

ATGAATATAA ATCTGCCGAA TTTCAATGTG AAGAACCTGG TGAAGGAGGC GGGCAGCACA 
ATATCACGAG TGGTGCAGCT CACGGAGGAG AAACTGGGCA CCACGGAGCG CACCGAGTAC 
GACTTGCACT TCCAGAATCT CGCCGAGCGG GCTGATGTGA CCAAGACATG GACGGAGAAG 
ATAGTGCGCG ACACAGAGTC GGTGCTGATA CCCAATCCTC AGAACCGGGT CGAAGACTTC 
ATCTTCGAGA AGATTGAAAA GTCGAAGCCA AAAAGGTTGA GCAACCTAGA GCACCTGGCT 
CTGGACATGA TCGAGGCTGG GGGCGATTTC GGGCAGGATT TACCCTATGG TCAGGCTCTG 
ATCAAGGTCG GCCAAGCGGA ACAGAAACTG GGCCAGTGCG AGCACGACTT TATAGCCACC 
TCGGGCATTT GCTTTACACA GCCGTTGCGC AAGTTCCTAG ACGGCGAAAT GAAGACAATT 
GGCAAGGAGC GAGGCATCCT GGAGACCAAG CGCCTGGATC TGGACGCCTG CAAGAACAGG 
GTGAAGAAAG CGCGCAGCAT GCTGGGTCAG CAATCGAAAG ATGGCATCTC GCCAGAGGCC 
GTCTTGGAAC AGGCGGAACG GGACTTGCGG GTGGCTCAGG CCGAGTTCGA CCGGCAGGCG 
GAGATTACCA AGCTGCTGCT GGACGGGATC AGCACATCGC AGGCATCTCA TTTGCGCCAC 
TTGCACGCCT TCATCCAGAC GCAAGTGCGC TACTACAAGC AGTGCGGCGA TGTCATGGAG 
CAGCTGCAGC GTGAACTGGC CAAAATGCAG CATCCCAAGC CACGCCTGCG TATCAATAGC 
GAAGACGTTG ATTGTGGGCC TCCCAGCTCG GTGTCGATGC ATTCTTGTGA TTCCGATTCA 
TTAGGGGGGA TAGCCCTAGA TAGCGATCCA GATCCCGATT TGGACAAGTC GCTTACGAAT 
CTGCTCGAAG ACTTTCACAT AGAATTCGAT ACGACAGCCG TCTCCACAGT GATCTTCGTG 
ACGGAGTGCT CACCAGTAAA CGAGGATTAC ATGTACGGAA AACAGGGTCT ATTGAAGGGT 
CTGGTGCCAC GCGCATTTGT TGAAATGCTC GACGAAGAGC ACGATGTCAC CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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EndoB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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