hppy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hppy
Entrez Gene ID	37203
Full Name	happyhour
Synonyms	CG7097,CG7097/hppy,Dmel\CG7097,EP-704,GLK/KHS1,Hppy,MAP4K3,anon-WO0153538.68,l(2)SH1261,l(2)SH2 1261
General protein information	Preferred Names happyhour Names CG7097 gene product from transcript CG7097-RC CG7097-PA CG7097-PB CG7097-PC CG7097-PD CG7097-PE gene 2 hppy-PA hppy-PB hppy-PC hppy-PD hppy-PE lethal (2) SH1261
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56C1-56C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_166335.3	NP_725863.1	happyhour, isoform A
NM_001299677.1	NP_001286606.1	happyhour, isoform E
NM_137555.3	NP_611399.2	happyhour, isoform B
NM_001299675.1	NP_001286604.1	happyhour, isoform C
NM_001299676.1	NP_001286605.1	happyhour, isoform D

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Map4k3	NP_596898.1
	LOC713952	XP_001103468.2
Gallus gallus (chicken)	MAP4K3	XP_003640980.2
Bos taurus (cattle)	MAP4K3	NP_001178091.1
Pan troglodytes (chimpanzee)	MAP4K3	XP_515427.4
Canis lupus familiaris (dog)	MAP4K3	XP_005630430.1
Mus musculus (house mouse)	Map4k3	NP_001074826.1
Drosophila melanogaster (fruit fly)	hppy	NP_611399.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010837	XP_309868.4
Caenorhabditis elegans (roundworm)	gck-2	NP_504721.1
Xenopus tropicalis (tropical clawed frog)	map4k3	NP_001123819.1
Homo sapiens (human)	MAP4K3	NP_003609.2
Danio rerio (zebrafish)	map4k3b	XP_001921635.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MAP4K3 has two independent functions during wing development, one related to activation of the JNK pathway in response to stress and other in assembling or activation of the TORC1 complex, modulating cellular responses to changes in nutrient availability.
Title: MAP4K3 is a component of the TORC1 signalling complex that modulates cell growth and viability in Drosophila melanogaster.

hppy expression triggers the activation of both the c-Jun N-terminal kinase (JNK) and target of rapamycin (TOR) signalling pathways.
Title: Drosophila happyhour modulates JNK-dependent apoptosis.

MAP4K3 regulates body size and metabolism in Drosophila
Title: MAP4K3 regulates body size and metabolism in Drosophila.

Genetic and biochemical experiments revealed that the epidermal growth factor (EGF)-signaling pathway regulates ethanol sensitivity in Drosophila and that happy hour (hppy, CG7097) functions as an inhibitor of the pathway.
Title: Happyhour, a Ste20 family kinase, implicates EGFR signaling in ethanol-induced behaviors.

The following hppy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hppy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10596	NM_137555.3 Latest version!	Drosophila melanogaster happyhour (hppy), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21867	NM_001299675.1 Latest version!	Drosophila melanogaster happyhour (hppy), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21869	NM_001299677.1 Latest version!	Drosophila melanogaster happyhour (hppy), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa10595	NM_166335.3 Latest version!	Drosophila melanogaster happyhour (hppy), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21868	NM_001299676.1 Latest version!	Drosophila melanogaster happyhour (hppy), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hppy ( NM_137555.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611399.2 Drosophila melanogaster happyhour (hppy), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10596
Clone ID Related Accession (Same CDS sequence)	NM_137555.3
Accession Version	NM_137555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	happyhour, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137555.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATGAG CCTCTTACAA TATATTGATG AGGAGCTGAA GCTAAGCGGC 
GATGCAGTCG TAGGCGGTAA CGGAGGCGGC AGCAGCAACA TAAGCAGCAG CGGAGGAGCA 
TCAAGTGGCA CCAATGGCCT CCTGGATAAG CACGAGCTAG ATGCTCTTCC CCAAGCGGCA 
ACAAAGTCGG CCGGCGATGG CTTCTCGCAT TCCAATAGCC CAACGGCGAA TTCCGGTGCG 
GGAGCGACCG GATCCAGGGA TAACGATGGA CCCTCGTCGA GCAACTCTTC GCATCTCTAC 
CAGAACCTGC TGAGAAGCAG CTCTGGTGAA ACGCCAGCGG GATCGAGCTC GGCGGGCAAT 
AACTGTGATT ACCGGCATGA GAACAACCAG AATGGTCTGG AGGACTCGCC TCGACGCCAT 
AGCTCCATGG ATCAGCTGAT TGGGTTGCTC GAGAACATGG GCAAGTCTCC GAGGACACGC 
AGCCTGAGCG ATGGTGGCAC CCAGGACGAT GACGAAGCGG AGAAGGAGGC GCAACCGGAT 
CTGCTGAACA ACACCCCACC GGTACCACCG AAGCGCTCCC ACAAACGCCG TCACACGCCA 
CCTCGACCCA TCTCCAACGG ACTGCCGCCC ACACCCAAGG TGCACATGGG CGCCTGCTTC 
TCCAAGATCT TCAACGGTTG CCCACTGCGC GTCCACTGCA CCGCCTCGTG GATTCATCCG 
GAGACGCGGG ACCAGCATCT GCTGATCGGC GCCGAGGAGG GCATCTACAA CCTCAACATG 
AACGAACTGC ATGACGCGGC CATCGATCAG TTGTTCCCGC GCCGCACCAC CTGGTTGTAT 
GTCATCAAGG ATGTGCTCAT GAGTTTGTCA GGAAAATCCT GCCAGCTCTA CAGGCACGAC 
CTGGTGGCCC TGCACTCCAA GCAGACGGTA CGCTTCTCGC TGCACATGAA CAAGATCCCG 
GAGCGACTGG TGCCGCGCAA GTTCGCTCTC ACCACCAAGG TGCCGGACAC AAAGTGCTGC 
ACACAGTGCT GTGTCACACG GAATCCGTAT AACGGATACA AATATCTCTG TGGGGCGACA 
CCCAGCGGCA TTTTCCTGAT GCAGTGGTAC GATCCACTGA ACAAGTTTAT GCTGCTCAAG 
CAGTGCGAGT GGCCAGCCAT CAGCATTCAG GGTGGTGGTC ATGGCTGCGT TCAGAACGGA 
CATACGCCCG TATTCGAGAT GATTATCACG CCGGAGTTGG AGTACCCGAT TGTGTGTACG 
GGTGTGCGAA AGGCCATGAA CGGCTGCCTC AAGCTGGAGC TGATCAACAT GAACAGTGCC 
AGCTGGTTCC ATTCGGAGGA CCTGGAATAT GACGCCATGG CCACGATGGT GCCGCGACGC 
GACCTGCTGA AGGTGGTGCG TGTGCACCAG GTGGAGAAGG ACGCCATTCT GGTCTGCTAC 
GGCAATCTGA TTCAGGTGGT CACCCTGCAG GGCAATCCCA AGCAGCACAA GAAGATGGTA 
TCGCAGCTCA ACTTTGATTT CAACGTGGAC AGCATTGTTT GTCTACCGGA CAGCGTTTTG 
GCATTCCATA AGCACGGCAT GCAGGGAAAG TCGTTGCGCA ATGGCGAGGT GACGCAGGAG 
ATCAAGGACA TGAGTCGCAC CTACAGGCTG CTGGGCAGCG ATAAGGTTGT GGCCTTGGAG 
AGCCAGCTGC TGAGGACCGG CTCCCTGGGC AGCGAGGAGG GACACGATCT GTACATTCTG 
GCCGGGCACG AGGCTAGCTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611399.2
CDS	370..3213
Translation	MAAAHSHHNANMLSSDISRRNPQDEYELIQKIGSGTYGDVYKAKRIQSNELAAIKVIKLEPSDDIQIIQQEIIMMRDCRHPNIIAYYGSYLRRDKLWICMEFCGGGSLQDIYQVTGPLTEVQIAYMCRETLKGLEYLHSMGKMHRDIKGANILLTEYGDVKLADFGVSAQITATINKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFDLHPMRALFLMSKSGFKPPTLNNKDKWSPTFHNFIKTALTKNPKKRPTAERLLQHPFVQCEMSLRVAKELLQKYQSPNPQFYYYLDGDEESVAGVPQRIASKMTSRTNGVPAQNHTLKTGMTTNSTWNERSSSPETLPSDMSLLQYIDEELKLSGDAVVGGNGGGSSNISSSGGASSGTNGLLDKHELDALPQAATKSAGDGFSHSNSPTANSGAGATGSRDNDGPSSSNSSHLYQNLLRSSSGETPAGSSSAGNNCDYRHENNQNGLEDSPRRHSSMDQLIGLLENMGKSPRTRSLSDGGTQDDDEAEKEAQPDLLNNTPPVPPKRSHKRRHTPPRPISNGLPPTPKVHMGACFSKIFNGCPLRVHCTASWIHPETRDQHLLIGAEEGIYNLNMNELHDAAIDQLFPRRTTWLYVIKDVLMSLSGKSCQLYRHDLVALHSKQTVRFSLHMNKIPERLVPRKFALTTKVPDTKCCTQCCVTRNPYNGYKYLCGATPSGIFLMQWYDPLNKFMLLKQCEWPAISIQGGGHGCVQNGHTPVFEMIITPELEYPIVCTGVRKAMNGCLKLELINMNSASWFHSEDLEYDAMATMVPRRDLLKVVRVHQVEKDAILVCYGNLIQVVTLQGNPKQHKKMVSQLNFDFNVDSIVCLPDSVLAFHKHGMQGKSLRNGEVTQEIKDMSRTYRLLGSDKVVALESQLLRTGSLGSEEGHDLYILAGHEASY

Target ORF information:

RefSeq Version	NM_137555.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster happyhour (hppy), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137555.3

ORF Insert Sequence:

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATGAG CCTCTTACAA TATATTGATG AGGAGCTGAA GCTAAGCGGC 
GATGCAGTCG TAGGCGGTAA CGGAGGCGGC AGCAGCAACA TAAGCAGCAG CGGAGGAGCA 
TCAAGTGGCA CCAATGGCCT CCTGGATAAG CACGAGCTAG ATGCTCTTCC CCAAGCGGCA 
ACAAAGTCGG CCGGCGATGG CTTCTCGCAT TCCAATAGCC CAACGGCGAA TTCCGGTGCG 
GGAGCGACCG GATCCAGGGA TAACGATGGA CCCTCGTCGA GCAACTCTTC GCATCTCTAC 
CAGAACCTGC TGAGAAGCAG CTCTGGTGAA ACGCCAGCGG GATCGAGCTC GGCGGGCAAT 
AACTGTGATT ACCGGCATGA GAACAACCAG AATGGTCTGG AGGACTCGCC TCGACGCCAT 
AGCTCCATGG ATCAGCTGAT TGGGTTGCTC GAGAACATGG GCAAGTCTCC GAGGACACGC 
AGCCTGAGCG ATGGTGGCAC CCAGGACGAT GACGAAGCGG AGAAGGAGGC GCAACCGGAT 
CTGCTGAACA ACACCCCACC GGTACCACCG AAGCGCTCCC ACAAACGCCG TCACACGCCA 
CCTCGACCCA TCTCCAACGG ACTGCCGCCC ACACCCAAGG TGCACATGGG CGCCTGCTTC 
TCCAAGATCT TCAACGGTTG CCCACTGCGC GTCCACTGCA CCGCCTCGTG GATTCATCCG 
GAGACGCGGG ACCAGCATCT GCTGATCGGC GCCGAGGAGG GCATCTACAA CCTCAACATG 
AACGAACTGC ATGACGCGGC CATCGATCAG TTGTTCCCGC GCCGCACCAC CTGGTTGTAT 
GTCATCAAGG ATGTGCTCAT GAGTTTGTCA GGAAAATCCT GCCAGCTCTA CAGGCACGAC 
CTGGTGGCCC TGCACTCCAA GCAGACGGTA CGCTTCTCGC TGCACATGAA CAAGATCCCG 
GAGCGACTGG TGCCGCGCAA GTTCGCTCTC ACCACCAAGG TGCCGGACAC AAAGTGCTGC 
ACACAGTGCT GTGTCACACG GAATCCGTAT AACGGATACA AATATCTCTG TGGGGCGACA 
CCCAGCGGCA TTTTCCTGAT GCAGTGGTAC GATCCACTGA ACAAGTTTAT GCTGCTCAAG 
CAGTGCGAGT GGCCAGCCAT CAGCATTCAG GGTGGTGGTC ATGGCTGCGT TCAGAACGGA 
CATACGCCCG TATTCGAGAT GATTATCACG CCGGAGTTGG AGTACCCGAT TGTGTGTACG 
GGTGTGCGAA AGGCCATGAA CGGCTGCCTC AAGCTGGAGC TGATCAACAT GAACAGTGCC 
AGCTGGTTCC ATTCGGAGGA CCTGGAATAT GACGCCATGG CCACGATGGT GCCGCGACGC 
GACCTGCTGA AGGTGGTGCG TGTGCACCAG GTGGAGAAGG ACGCCATTCT GGTCTGCTAC 
GGCAATCTGA TTCAGGTGGT CACCCTGCAG GGCAATCCCA AGCAGCACAA GAAGATGGTA 
TCGCAGCTCA ACTTTGATTT CAACGTGGAC AGCATTGTTT GTCTACCGGA CAGCGTTTTG 
GCATTCCATA AGCACGGCAT GCAGGGAAAG TCGTTGCGCA ATGGCGAGGT GACGCAGGAG 
ATCAAGGACA TGAGTCGCAC CTACAGGCTG CTGGGCAGCG ATAAGGTTGT GGCCTTGGAG 
AGCCAGCTGC TGAGGACCGG CTCCCTGGGC AGCGAGGAGG GACACGATCT GTACATTCTG 
GCCGGGCACG AGGCTAGCTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hppy ( NM_001299675.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286604.1 Drosophila melanogaster happyhour (hppy), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa21867
Clone ID Related Accession (Same CDS sequence)	NM_001299675.1
Accession Version	NM_001299675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	happyhour, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATTCC CAGCGCCGAG CGATCAAAAC CGAAATTGAG CGGCACCCTG 
ATTATGAACA TGTCCTTAAG GAGCCTCTTA CAATATATTG ATGAGGAGCT GAAGCTAAGC 
GGCGATGCAG TCGTAGGCGG TAACGGAGGC GGCAGCAGCA ACATAAGCAG CAGCGGAGGA 
GCATCAAGTG GCACCAATGG CCTCCTGGAT AAGCACGAGC TAGATGCTCT TCCCCAAGCG 
GCAACAAAGT CGGCCGGCGA TGGCTTCTCG CATTCCAATA GCCCAACGGC GAATTCCGGT 
GCGGGAGCGA CCGGATCCAG GGATAACGAT GGACCCTCGT CGAGCAACTC TTCGCATCTC 
TACCAGAACC TGCTGAGAAG CAGCTCTGGT GAAACGCCAG CGGGATCGAG CTCGGCGGGC 
AATAACTGTG ATTACCGGCA TGAGAACAAC CAGAATGGTC TGGAGGACTC GCCTCGACGC 
CATAGCTCCA TGGATCAGCT GATTGGGTTG CTCGAGAACA TGGGCAAGTC TCCGAGGACA 
CGCAGCCTGA GCGATGGTGG CACCCAGGAC GATGACGAAG CGGAGAAGGA GGCGCAACCG 
GATCTGCTGA ACAACACCCC ACCGGTACCA CCGAAGCGCT CCCACAAACG CCGTCACACG 
CCACCTCGAC CCATCTCCAA CGGACTGCCG CCCACACCCA AGGTGCACAT GGGCGCCTGC 
TTCTCCAAGA TCTTCAACGG TTGCCCACTG CGCGTCCACT GCACCGCCTC GTGGATTCAT 
CCGGAGACGC GGGACCAGCA TCTGCTGATC GGCGCCGAGG AGGGCATCTA CAACCTCAAC 
ATGAACGAAC TGCATGACGC GGCCATCGAT CAGTTGTTCC CGCGCCGCAC CACCTGGTTG 
TATGTCATCA AGGATGTGCT CATGAGTTTG TCAGGAAAAT CCTGCCAGCT CTACAGGCAC 
GACCTGGTGG CCCTGCACTC CAAGCAGACG GTACGCTTCT CGCTGCACAT GAACAAGATC 
CCGGAGCGAC TGGTGCCGCG CAAGTTCGCT CTCACCACCA AGGTGCCGGA CACAAAGTGC 
TGCACACAGT GCTGTGTCAC ACGGAATCCG TATAACGGAT ACAAATATCT CTGTGGGGCG 
ACACCCAGCG GCATTTTCCT GATGCAGTGG TACGATCCAC TGAACAAGTT TATGCTGCTC 
AAGCAGTGCG AGTGGCCAGC CATCAGCATT CAGGGTGGTG GTCATGGCTG CGTTCAGAAC 
GGACATACGC CCGTATTCGA GATGATTATC ACGCCGGAGT TGGAGTACCC GATTGTGTGT 
ACGGGTGTGC GAAAGGCCAT GAACGGCTGC CTCAAGCTGG AGCTGATCAA CATGAACAGT 
GCCAGCTGGT TCCATTCGGA GGACCTGGAA TATGACGCCA TGGCCACGAT GGTGCCGCGA 
CGCGACCTGC TGAAGGTGGT GCGTGTGCAC CAGGTGGAGA AGGACGCCAT TCTGGTCTGC 
TACGGCAATC TGATTCAGGT GGTCACCCTG CAGGGCAATC CCAAGCAGCA CAAGAAGATG 
GTATCGCAGC TCAACTTTGA TTTCAACGTG GACAGCATTG TTTGTCTACC GGACAGCGTT 
TTGGCATTCC ATAAGCACGG CATGCAGGGA AAGTCGTTGC GCAATGGCGA GGTGACGCAG 
GAGATCAAGG ACATGAGTCG CACCTACAGG CTGCTGGGCA GCGATAAGGT TGTGGCCTTG 
GAGAGCCAGC TGCTGAGGAC CGGCTCCCTG GGCAGCGAGG AGGGACACGA TCTGTACATT 
CTGGCCGGGC ACGAGGCTAG CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286604.1
CDS	370..3276
Translation	MAAAHSHHNANMLSSDISRRNPQDEYELIQKIGSGTYGDVYKAKRIQSNELAAIKVIKLEPSDDIQIIQQEIIMMRDCRHPNIIAYYGSYLRRDKLWICMEFCGGGSLQDIYQVTGPLTEVQIAYMCRETLKGLEYLHSMGKMHRDIKGANILLTEYGDVKLADFGVSAQITATINKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFDLHPMRALFLMSKSGFKPPTLNNKDKWSPTFHNFIKTALTKNPKKRPTAERLLQHPFVQCEMSLRVAKELLQKYQSPNPQFYYYLDGDEESVAGVPQRIASKMTSRTNGVPAQNHTLKTGMTTNSTWNERSSSPETLPSDIPSAERSKPKLSGTLIMNMSLRSLLQYIDEELKLSGDAVVGGNGGGSSNISSSGGASSGTNGLLDKHELDALPQAATKSAGDGFSHSNSPTANSGAGATGSRDNDGPSSSNSSHLYQNLLRSSSGETPAGSSSAGNNCDYRHENNQNGLEDSPRRHSSMDQLIGLLENMGKSPRTRSLSDGGTQDDDEAEKEAQPDLLNNTPPVPPKRSHKRRHTPPRPISNGLPPTPKVHMGACFSKIFNGCPLRVHCTASWIHPETRDQHLLIGAEEGIYNLNMNELHDAAIDQLFPRRTTWLYVIKDVLMSLSGKSCQLYRHDLVALHSKQTVRFSLHMNKIPERLVPRKFALTTKVPDTKCCTQCCVTRNPYNGYKYLCGATPSGIFLMQWYDPLNKFMLLKQCEWPAISIQGGGHGCVQNGHTPVFEMIITPELEYPIVCTGVRKAMNGCLKLELINMNSASWFHSEDLEYDAMATMVPRRDLLKVVRVHQVEKDAILVCYGNLIQVVTLQGNPKQHKKMVSQLNFDFNVDSIVCLPDSVLAFHKHGMQGKSLRNGEVTQEIKDMSRTYRLLGSDKVVALESQLLRTGSLGSEEGHDLYILAGHEASY

Target ORF information:

RefSeq Version	NM_001299675.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster happyhour (hppy), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299675.1

ORF Insert Sequence:

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATTCC CAGCGCCGAG CGATCAAAAC CGAAATTGAG CGGCACCCTG 
ATTATGAACA TGTCCTTAAG GAGCCTCTTA CAATATATTG ATGAGGAGCT GAAGCTAAGC 
GGCGATGCAG TCGTAGGCGG TAACGGAGGC GGCAGCAGCA ACATAAGCAG CAGCGGAGGA 
GCATCAAGTG GCACCAATGG CCTCCTGGAT AAGCACGAGC TAGATGCTCT TCCCCAAGCG 
GCAACAAAGT CGGCCGGCGA TGGCTTCTCG CATTCCAATA GCCCAACGGC GAATTCCGGT 
GCGGGAGCGA CCGGATCCAG GGATAACGAT GGACCCTCGT CGAGCAACTC TTCGCATCTC 
TACCAGAACC TGCTGAGAAG CAGCTCTGGT GAAACGCCAG CGGGATCGAG CTCGGCGGGC 
AATAACTGTG ATTACCGGCA TGAGAACAAC CAGAATGGTC TGGAGGACTC GCCTCGACGC 
CATAGCTCCA TGGATCAGCT GATTGGGTTG CTCGAGAACA TGGGCAAGTC TCCGAGGACA 
CGCAGCCTGA GCGATGGTGG CACCCAGGAC GATGACGAAG CGGAGAAGGA GGCGCAACCG 
GATCTGCTGA ACAACACCCC ACCGGTACCA CCGAAGCGCT CCCACAAACG CCGTCACACG 
CCACCTCGAC CCATCTCCAA CGGACTGCCG CCCACACCCA AGGTGCACAT GGGCGCCTGC 
TTCTCCAAGA TCTTCAACGG TTGCCCACTG CGCGTCCACT GCACCGCCTC GTGGATTCAT 
CCGGAGACGC GGGACCAGCA TCTGCTGATC GGCGCCGAGG AGGGCATCTA CAACCTCAAC 
ATGAACGAAC TGCATGACGC GGCCATCGAT CAGTTGTTCC CGCGCCGCAC CACCTGGTTG 
TATGTCATCA AGGATGTGCT CATGAGTTTG TCAGGAAAAT CCTGCCAGCT CTACAGGCAC 
GACCTGGTGG CCCTGCACTC CAAGCAGACG GTACGCTTCT CGCTGCACAT GAACAAGATC 
CCGGAGCGAC TGGTGCCGCG CAAGTTCGCT CTCACCACCA AGGTGCCGGA CACAAAGTGC 
TGCACACAGT GCTGTGTCAC ACGGAATCCG TATAACGGAT ACAAATATCT CTGTGGGGCG 
ACACCCAGCG GCATTTTCCT GATGCAGTGG TACGATCCAC TGAACAAGTT TATGCTGCTC 
AAGCAGTGCG AGTGGCCAGC CATCAGCATT CAGGGTGGTG GTCATGGCTG CGTTCAGAAC 
GGACATACGC CCGTATTCGA GATGATTATC ACGCCGGAGT TGGAGTACCC GATTGTGTGT 
ACGGGTGTGC GAAAGGCCAT GAACGGCTGC CTCAAGCTGG AGCTGATCAA CATGAACAGT 
GCCAGCTGGT TCCATTCGGA GGACCTGGAA TATGACGCCA TGGCCACGAT GGTGCCGCGA 
CGCGACCTGC TGAAGGTGGT GCGTGTGCAC CAGGTGGAGA AGGACGCCAT TCTGGTCTGC 
TACGGCAATC TGATTCAGGT GGTCACCCTG CAGGGCAATC CCAAGCAGCA CAAGAAGATG 
GTATCGCAGC TCAACTTTGA TTTCAACGTG GACAGCATTG TTTGTCTACC GGACAGCGTT 
TTGGCATTCC ATAAGCACGG CATGCAGGGA AAGTCGTTGC GCAATGGCGA GGTGACGCAG 
GAGATCAAGG ACATGAGTCG CACCTACAGG CTGCTGGGCA GCGATAAGGT TGTGGCCTTG 
GAGAGCCAGC TGCTGAGGAC CGGCTCCCTG GGCAGCGAGG AGGGACACGA TCTGTACATT 
CTGGCCGGGC ACGAGGCTAG CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hppy ( NM_001299677.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286606.1 Drosophila melanogaster happyhour (hppy), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa21869
Clone ID Related Accession (Same CDS sequence)	NM_001299677.1
Accession Version	NM_001299677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	happyhour, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATCGG CGATGCAGTC GTAGGCGGTA ACGGAGGCGG CAGCAGCAAC 
ATAAGCAGCA GCGGAGGAGC ATCAAGTGGC ACCAATGGCC TCCTGGATAA GCACGAGCTA 
GATGCTCTTC CCCAAGCGGC AACAAAGTCG GCCGGCGATG GCTTCTCGCA TTCCAATAGC 
CCAACGGCGA ATTCCGGTGC GGGAGCGACC GGATCCAGGG ATAACGATGG ACCCTCGTCG 
AGCAACTCTT CGCATCTCTA CCAGAACCTG CTGAGAAGCA GCTCTGGTGA AACGCCAGCG 
GGATCGAGCT CGGCGGGCAA TAACTGTGAT TACCGGCATG AGAACAACCA GAATGGTCTG 
GAGGACTCGC CTCGACGCCA TAGCTCCATG GATCAGCTGA TTGGGTTGCT CGAGAACATG 
GGCAAGTCTC CGAGGACACG CAGCCTGAGC GATGGTGGCA CCCAGGACGA TGACGAAGCG 
GAGAAGGAGG CGCAACCGGA TCTGCTGAAC AACACCCCAC CGGTACCACC GAAGCGCTCC 
CACAAACGCC GTCACACGCC ACCTCGACCC ATCTCCAACG GACTGCCGCC CACACCCAAG 
GTGCACATGG GCGCCTGCTT CTCCAAGATC TTCAACGGTT GCCCACTGCG CGTCCACTGC 
ACCGCCTCGT GGATTCATCC GGAGACGCGG GACCAGCATC TGCTGATCGG CGCCGAGGAG 
GGCATCTACA ACCTCAACAT GAACGAACTG CATGACGCGG CCATCGATCA GTTGTTCCCG 
CGCCGCACCA CCTGGTTGTA TGTCATCAAG GATGTGCTCA TGAGTTTGTC AGGAAAATCC 
TGCCAGCTCT ACAGGCACGA CCTGGTGGCC CTGCACTCCA AGCAGACGGT ACGCTTCTCG 
CTGCACATGA ACAAGATCCC GGAGCGACTG GTGCCGCGCA AGTTCGCTCT CACCACCAAG 
GTGCCGGACA CAAAGTGCTG CACACAGTGC TGTGTCACAC GGAATCCGTA TAACGGATAC 
AAATATCTCT GTGGGGCGAC ACCCAGCGGC ATTTTCCTGA TGCAGTGGTA CGATCCACTG 
AACAAGTTTA TGCTGCTCAA GCAGTGCGAG TGGCCAGCCA TCAGCATTCA GGGTGGTGGT 
CATGGCTGCG TTCAGAACGG ACATACGCCC GTATTCGAGA TGATTATCAC GCCGGAGTTG 
GAGTACCCGA TTGTGTGTAC GGGTGTGCGA AAGGCCATGA ACGGCTGCCT CAAGCTGGAG 
CTGATCAACA TGAACAGTGC CAGCTGGTTC CATTCGGAGG ACCTGGAATA TGACGCCATG 
GCCACGATGG TGCCGCGACG CGACCTGCTG AAGGTGGTGC GTGTGCACCA GGTGGAGAAG 
GACGCCATTC TGGTCTGCTA CGGCAATCTG ATTCAGGTGG TCACCCTGCA GGGCAATCCC 
AAGCAGCACA AGAAGATGGT ATCGCAGCTC AACTTTGATT TCAACGTGGA CAGCATTGTT 
TGTCTACCGG ACAGCGTTTT GGCATTCCAT AAGCACGGCA TGCAGGGAAA GTCGTTGCGC 
AATGGCGAGG TGACGCAGGA GATCAAGGAC ATGAGTCGCA CCTACAGGCT GCTGGGCAGC 
GATAAGGTTG TGGCCTTGGA GAGCCAGCTG CTGAGGACCG GCTCCCTGGG CAGCGAGGAG 
GGACACGATC TGTACATTCT GGCCGGGCAC GAGGCTAGCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286606.1
CDS	370..3174
Translation	MAAAHSHHNANMLSSDISRRNPQDEYELIQKIGSGTYGDVYKAKRIQSNELAAIKVIKLEPSDDIQIIQQEIIMMRDCRHPNIIAYYGSYLRRDKLWICMEFCGGGSLQDIYQVTGPLTEVQIAYMCRETLKGLEYLHSMGKMHRDIKGANILLTEYGDVKLADFGVSAQITATINKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFDLHPMRALFLMSKSGFKPPTLNNKDKWSPTFHNFIKTALTKNPKKRPTAERLLQHPFVQCEMSLRVAKELLQKYQSPNPQFYYYLDGDEESVAGVPQRIASKMTSRTNGVPAQNHTLKTGMTTNSTWNERSSSPETLPSDIGDAVVGGNGGGSSNISSSGGASSGTNGLLDKHELDALPQAATKSAGDGFSHSNSPTANSGAGATGSRDNDGPSSSNSSHLYQNLLRSSSGETPAGSSSAGNNCDYRHENNQNGLEDSPRRHSSMDQLIGLLENMGKSPRTRSLSDGGTQDDDEAEKEAQPDLLNNTPPVPPKRSHKRRHTPPRPISNGLPPTPKVHMGACFSKIFNGCPLRVHCTASWIHPETRDQHLLIGAEEGIYNLNMNELHDAAIDQLFPRRTTWLYVIKDVLMSLSGKSCQLYRHDLVALHSKQTVRFSLHMNKIPERLVPRKFALTTKVPDTKCCTQCCVTRNPYNGYKYLCGATPSGIFLMQWYDPLNKFMLLKQCEWPAISIQGGGHGCVQNGHTPVFEMIITPELEYPIVCTGVRKAMNGCLKLELINMNSASWFHSEDLEYDAMATMVPRRDLLKVVRVHQVEKDAILVCYGNLIQVVTLQGNPKQHKKMVSQLNFDFNVDSIVCLPDSVLAFHKHGMQGKSLRNGEVTQEIKDMSRTYRLLGSDKVVALESQLLRTGSLGSEEGHDLYILAGHEASY

Target ORF information:

RefSeq Version	NM_001299677.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster happyhour (hppy), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299677.1

ORF Insert Sequence:

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATCGG CGATGCAGTC GTAGGCGGTA ACGGAGGCGG CAGCAGCAAC 
ATAAGCAGCA GCGGAGGAGC ATCAAGTGGC ACCAATGGCC TCCTGGATAA GCACGAGCTA 
GATGCTCTTC CCCAAGCGGC AACAAAGTCG GCCGGCGATG GCTTCTCGCA TTCCAATAGC 
CCAACGGCGA ATTCCGGTGC GGGAGCGACC GGATCCAGGG ATAACGATGG ACCCTCGTCG 
AGCAACTCTT CGCATCTCTA CCAGAACCTG CTGAGAAGCA GCTCTGGTGA AACGCCAGCG 
GGATCGAGCT CGGCGGGCAA TAACTGTGAT TACCGGCATG AGAACAACCA GAATGGTCTG 
GAGGACTCGC CTCGACGCCA TAGCTCCATG GATCAGCTGA TTGGGTTGCT CGAGAACATG 
GGCAAGTCTC CGAGGACACG CAGCCTGAGC GATGGTGGCA CCCAGGACGA TGACGAAGCG 
GAGAAGGAGG CGCAACCGGA TCTGCTGAAC AACACCCCAC CGGTACCACC GAAGCGCTCC 
CACAAACGCC GTCACACGCC ACCTCGACCC ATCTCCAACG GACTGCCGCC CACACCCAAG 
GTGCACATGG GCGCCTGCTT CTCCAAGATC TTCAACGGTT GCCCACTGCG CGTCCACTGC 
ACCGCCTCGT GGATTCATCC GGAGACGCGG GACCAGCATC TGCTGATCGG CGCCGAGGAG 
GGCATCTACA ACCTCAACAT GAACGAACTG CATGACGCGG CCATCGATCA GTTGTTCCCG 
CGCCGCACCA CCTGGTTGTA TGTCATCAAG GATGTGCTCA TGAGTTTGTC AGGAAAATCC 
TGCCAGCTCT ACAGGCACGA CCTGGTGGCC CTGCACTCCA AGCAGACGGT ACGCTTCTCG 
CTGCACATGA ACAAGATCCC GGAGCGACTG GTGCCGCGCA AGTTCGCTCT CACCACCAAG 
GTGCCGGACA CAAAGTGCTG CACACAGTGC TGTGTCACAC GGAATCCGTA TAACGGATAC 
AAATATCTCT GTGGGGCGAC ACCCAGCGGC ATTTTCCTGA TGCAGTGGTA CGATCCACTG 
AACAAGTTTA TGCTGCTCAA GCAGTGCGAG TGGCCAGCCA TCAGCATTCA GGGTGGTGGT 
CATGGCTGCG TTCAGAACGG ACATACGCCC GTATTCGAGA TGATTATCAC GCCGGAGTTG 
GAGTACCCGA TTGTGTGTAC GGGTGTGCGA AAGGCCATGA ACGGCTGCCT CAAGCTGGAG 
CTGATCAACA TGAACAGTGC CAGCTGGTTC CATTCGGAGG ACCTGGAATA TGACGCCATG 
GCCACGATGG TGCCGCGACG CGACCTGCTG AAGGTGGTGC GTGTGCACCA GGTGGAGAAG 
GACGCCATTC TGGTCTGCTA CGGCAATCTG ATTCAGGTGG TCACCCTGCA GGGCAATCCC 
AAGCAGCACA AGAAGATGGT ATCGCAGCTC AACTTTGATT TCAACGTGGA CAGCATTGTT 
TGTCTACCGG ACAGCGTTTT GGCATTCCAT AAGCACGGCA TGCAGGGAAA GTCGTTGCGC 
AATGGCGAGG TGACGCAGGA GATCAAGGAC ATGAGTCGCA CCTACAGGCT GCTGGGCAGC 
GATAAGGTTG TGGCCTTGGA GAGCCAGCTG CTGAGGACCG GCTCCCTGGG CAGCGAGGAG 
GGACACGATC TGTACATTCT GGCCGGGCAC GAGGCTAGCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hppy ( NM_166335.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725863.1 Drosophila melanogaster happyhour (hppy), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10595
Clone ID Related Accession (Same CDS sequence)	NM_166335.3
Accession Version	NM_166335.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	happyhour, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166335.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATGAG CCTCTTACAA TATATTGATG AGGAGCTGAA GCTAAGAGCG 
ACCTTGCCAC TGAACAACGA CACCAAAGAT CCACTCGGCG CCGAGTGCAG CTGCTCCTCC 
CACAATGGAG GAGCCGCCGG AGGAGGAGGA GGAGGAGGAG TTGGAGTAGG AGCAGGCGGA 
GCAGCCGCGA ACGGCAGCAG CAGCAGCAGC GGAGGCGCAA CAGTCGGCAC CACTCATCAT 
CAGCACCAAC AGCACCACCA GCATCACCAC CATCCGAATC ATCTGCATCA GCATCAGTCC 
CATCAATTGC CGCAACAGCA GCAGCAGCAG TCACATCAGG CGGCACAGCA GGAACACCAT 
CACCACCATC CAATGACCTC GTCCATATCC AGCGGCTTGG TGACCGCCAA TGCCAACAAC 
ATCAGCTCGT CCGCCTCGCT GGCCTCCATG CCAGGTCTCA GTGCCTACCT GGGCATGCGC 
TCCCATGTGG TTGGCCACAT GGGTATGGGA TTTGGCATGG GTCTCGGCAT GTCCAACCTG 
CGCACCACCA GCATCGATGC CGACGACGAT GAACTGGTGG CTGCCGATGT GGCCATGAAT 
AATGCTGCTG CCGCCGTTCC TGGGAACGGA TCCGGTGCTG GCAACGGTTC CGGATCCGGA 
TGCTCCGCCT CTGCGGCGCA CTATCTGCGT TACAGCAGCA ACTACCGATC CGCCGCCGCG 
GCTCAGGCAT CGCAGGCGGC ACATCCGCAC GTTAACTCCA ATTCGAATTC GAACAACGGC 
CATGGCCATG CGCCGATCAC ATCCAGCATC TCGTCCTCTG CGTCATCAGC TTCGTTTTAT 
AATCGCCTCC TACTCCTCGA TAATTCCAGC GGCGATGCAG TCGTAGGCGG TAACGGAGGC 
GGCAGCAGCA ACATAAGCAG CAGCGGAGGA GCATCAAGTG GCACCAATGG CCTCCTGGAT 
AAGCACGAGC TAGATGCTCT TCCCCAAGCG GCAACAAAGT CGGCCGGCGA TGGCTTCTCG 
CATTCCAATA GCCCAACGGC GAATTCCGGT GCGGGAGCGA CCGGATCCAG GGATAACGAT 
GGACCCTCGT CGAGCAACTC TTCGCATCTC TACCAGAACC TGCTGAGAAG CAGCTCTGGT 
GAAACGCCAG CGGGATCGAG CTCGGCGGGC AATAACTGTG ATTACCGGCA TGAGAACAAC 
CAGAATGGTC TGGAGGACTC GCCTCGACGC CATAGCTCCA TGGATCAGCT GATTGGGTTG 
CTCGAGAACA TGGGCAAGTC TCCGAGGACA CGCAGCCTGA GCGATGGTGG CACCCAGGAC 
GATGACGAAG CGGAGAAGGA GGCGCAACCG GATCTGCTGA ACAACACCCC ACCGGTACCA 
CCGAAGCGCT CCCACAAACG CCGTCACACG CCACCTCGAC CCATCTCCAA CGGACTGCCG 
CCCACACCCA AGGTGCACAT GGGCGCCTGC TTCTCCAAGA TCTTCAACGG TTGCCCACTG 
CGCGTCCACT GCACCGCCTC GTGGATTCAT CCGGAGACGC GGGACCAGCA TCTGCTGATC 
GGCGCCGAGG AGGGCATCTA CAACCTCAAC ATGAACGAAC TGCATGACGC GGCCATCGAT 
CAGTTGTTCC CGCGCCGCAC CACCTGGTTG TATGTCATCA AGGATGTGCT CATGAGTTTG 
TCAGGAAAAT CCTGCCAGCT CTACAGGCAC GACCTGGTGG CCCTGCACTC CAAGCAGACG 
GTACGCTTCT CGCTGCACAT GAACAAGATC CCGGAGCGAC TGGTGCCGCG CAAGTTCGCT 
CTCACCACCA AGGTGCCGGA CACAAAGTGC TGCACACAGT GCTGTGTCAC ACGGAATCCG 
TATAACGGAT ACAAATATCT CTGTGGGGCG ACACCCAGCG GCATTTTCCT GATGCAGTGG 
TACGATCCAC TGAACAAGTT TATGCTGCTC AAGCAGTGCG AGTGGCCAGC CATCAGCATT 
CAGGGTGGTG GTCATGGCTG CGTTCAGAAC GGACATACGC CCGTATTCGA GATGATTATC 
ACGCCGGAGT TGGAGTACCC GATTGTGTGT ACGGGTGTGC GAAAGGCCAT GAACGGCTGC 
CTCAAGCTGG AGCTGATCAA CATGAACAGT GCCAGCTGGT TCCATTCGGA GGACCTGGAA 
TATGACGCCA TGGCCACGAT GGTGCCGCGA CGCGACCTGC TGAAGGTGGT GCGTGTGCAC 
CAGGTGGAGA AGGACGCCAT TCTGGTCTGC TACGGCAATC TGATTCAGGT GGTCACCCTG 
CAGGGCAATC CCAAGCAGCA CAAGAAGATG GTATCGCAGC TCAACTTTGA TTTCAACGTG 
GACAGCATTG TTTGTCTACC GGACAGCGTT TTGGCATTCC ATAAGCACGG CATGCAGGGA 
AAGTCGTTGC GCAATGGCGA GGTGACGCAG GAGATCAAGG ACATGAGTCG CACCTACAGG 
CTGCTGGGCA GCGATAAGGT TGTGGCCTTG GAGAGCCAGC TGCTGAGGAC CGGCTCCCTG 
GGCAGCGAGG AGGGACACGA TCTGTACATT CTGGCCGGGC ACGAGGCTAG CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725863.1
CDS	370..4026
Translation	MAAAHSHHNANMLSSDISRRNPQDEYELIQKIGSGTYGDVYKAKRIQSNELAAIKVIKLEPSDDIQIIQQEIIMMRDCRHPNIIAYYGSYLRRDKLWICMEFCGGGSLQDIYQVTGPLTEVQIAYMCRETLKGLEYLHSMGKMHRDIKGANILLTEYGDVKLADFGVSAQITATINKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFDLHPMRALFLMSKSGFKPPTLNNKDKWSPTFHNFIKTALTKNPKKRPTAERLLQHPFVQCEMSLRVAKELLQKYQSPNPQFYYYLDGDEESVAGVPQRIASKMTSRTNGVPAQNHTLKTGMTTNSTWNERSSSPETLPSDMSLLQYIDEELKLRATLPLNNDTKDPLGAECSCSSHNGGAAGGGGGGGVGVGAGGAAANGSSSSSGGATVGTTHHQHQQHHQHHHHPNHLHQHQSHQLPQQQQQQSHQAAQQEHHHHHPMTSSISSGLVTANANNISSSASLASMPGLSAYLGMRSHVVGHMGMGFGMGLGMSNLRTTSIDADDDELVAADVAMNNAAAAVPGNGSGAGNGSGSGCSASAAHYLRYSSNYRSAAAAQASQAAHPHVNSNSNSNNGHGHAPITSSISSSASSASFYNRLLLLDNSSGDAVVGGNGGGSSNISSSGGASSGTNGLLDKHELDALPQAATKSAGDGFSHSNSPTANSGAGATGSRDNDGPSSSNSSHLYQNLLRSSSGETPAGSSSAGNNCDYRHENNQNGLEDSPRRHSSMDQLIGLLENMGKSPRTRSLSDGGTQDDDEAEKEAQPDLLNNTPPVPPKRSHKRRHTPPRPISNGLPPTPKVHMGACFSKIFNGCPLRVHCTASWIHPETRDQHLLIGAEEGIYNLNMNELHDAAIDQLFPRRTTWLYVIKDVLMSLSGKSCQLYRHDLVALHSKQTVRFSLHMNKIPERLVPRKFALTTKVPDTKCCTQCCVTRNPYNGYKYLCGATPSGIFLMQWYDPLNKFMLLKQCEWPAISIQGGGHGCVQNGHTPVFEMIITPELEYPIVCTGVRKAMNGCLKLELINMNSASWFHSEDLEYDAMATMVPRRDLLKVVRVHQVEKDAILVCYGNLIQVVTLQGNPKQHKKMVSQLNFDFNVDSIVCLPDSVLAFHKHGMQGKSLRNGEVTQEIKDMSRTYRLLGSDKVVALESQLLRTGSLGSEEGHDLYILAGHEASY

Target ORF information:

RefSeq Version	NM_166335.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster happyhour (hppy), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166335.3

ORF Insert Sequence:

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATGAG CCTCTTACAA TATATTGATG AGGAGCTGAA GCTAAGAGCG 
ACCTTGCCAC TGAACAACGA CACCAAAGAT CCACTCGGCG CCGAGTGCAG CTGCTCCTCC 
CACAATGGAG GAGCCGCCGG AGGAGGAGGA GGAGGAGGAG TTGGAGTAGG AGCAGGCGGA 
GCAGCCGCGA ACGGCAGCAG CAGCAGCAGC GGAGGCGCAA CAGTCGGCAC CACTCATCAT 
CAGCACCAAC AGCACCACCA GCATCACCAC CATCCGAATC ATCTGCATCA GCATCAGTCC 
CATCAATTGC CGCAACAGCA GCAGCAGCAG TCACATCAGG CGGCACAGCA GGAACACCAT 
CACCACCATC CAATGACCTC GTCCATATCC AGCGGCTTGG TGACCGCCAA TGCCAACAAC 
ATCAGCTCGT CCGCCTCGCT GGCCTCCATG CCAGGTCTCA GTGCCTACCT GGGCATGCGC 
TCCCATGTGG TTGGCCACAT GGGTATGGGA TTTGGCATGG GTCTCGGCAT GTCCAACCTG 
CGCACCACCA GCATCGATGC CGACGACGAT GAACTGGTGG CTGCCGATGT GGCCATGAAT 
AATGCTGCTG CCGCCGTTCC TGGGAACGGA TCCGGTGCTG GCAACGGTTC CGGATCCGGA 
TGCTCCGCCT CTGCGGCGCA CTATCTGCGT TACAGCAGCA ACTACCGATC CGCCGCCGCG 
GCTCAGGCAT CGCAGGCGGC ACATCCGCAC GTTAACTCCA ATTCGAATTC GAACAACGGC 
CATGGCCATG CGCCGATCAC ATCCAGCATC TCGTCCTCTG CGTCATCAGC TTCGTTTTAT 
AATCGCCTCC TACTCCTCGA TAATTCCAGC GGCGATGCAG TCGTAGGCGG TAACGGAGGC 
GGCAGCAGCA ACATAAGCAG CAGCGGAGGA GCATCAAGTG GCACCAATGG CCTCCTGGAT 
AAGCACGAGC TAGATGCTCT TCCCCAAGCG GCAACAAAGT CGGCCGGCGA TGGCTTCTCG 
CATTCCAATA GCCCAACGGC GAATTCCGGT GCGGGAGCGA CCGGATCCAG GGATAACGAT 
GGACCCTCGT CGAGCAACTC TTCGCATCTC TACCAGAACC TGCTGAGAAG CAGCTCTGGT 
GAAACGCCAG CGGGATCGAG CTCGGCGGGC AATAACTGTG ATTACCGGCA TGAGAACAAC 
CAGAATGGTC TGGAGGACTC GCCTCGACGC CATAGCTCCA TGGATCAGCT GATTGGGTTG 
CTCGAGAACA TGGGCAAGTC TCCGAGGACA CGCAGCCTGA GCGATGGTGG CACCCAGGAC 
GATGACGAAG CGGAGAAGGA GGCGCAACCG GATCTGCTGA ACAACACCCC ACCGGTACCA 
CCGAAGCGCT CCCACAAACG CCGTCACACG CCACCTCGAC CCATCTCCAA CGGACTGCCG 
CCCACACCCA AGGTGCACAT GGGCGCCTGC TTCTCCAAGA TCTTCAACGG TTGCCCACTG 
CGCGTCCACT GCACCGCCTC GTGGATTCAT CCGGAGACGC GGGACCAGCA TCTGCTGATC 
GGCGCCGAGG AGGGCATCTA CAACCTCAAC ATGAACGAAC TGCATGACGC GGCCATCGAT 
CAGTTGTTCC CGCGCCGCAC CACCTGGTTG TATGTCATCA AGGATGTGCT CATGAGTTTG 
TCAGGAAAAT CCTGCCAGCT CTACAGGCAC GACCTGGTGG CCCTGCACTC CAAGCAGACG 
GTACGCTTCT CGCTGCACAT GAACAAGATC CCGGAGCGAC TGGTGCCGCG CAAGTTCGCT 
CTCACCACCA AGGTGCCGGA CACAAAGTGC TGCACACAGT GCTGTGTCAC ACGGAATCCG 
TATAACGGAT ACAAATATCT CTGTGGGGCG ACACCCAGCG GCATTTTCCT GATGCAGTGG 
TACGATCCAC TGAACAAGTT TATGCTGCTC AAGCAGTGCG AGTGGCCAGC CATCAGCATT 
CAGGGTGGTG GTCATGGCTG CGTTCAGAAC GGACATACGC CCGTATTCGA GATGATTATC 
ACGCCGGAGT TGGAGTACCC GATTGTGTGT ACGGGTGTGC GAAAGGCCAT GAACGGCTGC 
CTCAAGCTGG AGCTGATCAA CATGAACAGT GCCAGCTGGT TCCATTCGGA GGACCTGGAA 
TATGACGCCA TGGCCACGAT GGTGCCGCGA CGCGACCTGC TGAAGGTGGT GCGTGTGCAC 
CAGGTGGAGA AGGACGCCAT TCTGGTCTGC TACGGCAATC TGATTCAGGT GGTCACCCTG 
CAGGGCAATC CCAAGCAGCA CAAGAAGATG GTATCGCAGC TCAACTTTGA TTTCAACGTG 
GACAGCATTG TTTGTCTACC GGACAGCGTT TTGGCATTCC ATAAGCACGG CATGCAGGGA 
AAGTCGTTGC GCAATGGCGA GGTGACGCAG GAGATCAAGG ACATGAGTCG CACCTACAGG 
CTGCTGGGCA GCGATAAGGT TGTGGCCTTG GAGAGCCAGC TGCTGAGGAC CGGCTCCCTG 
GGCAGCGAGG AGGGACACGA TCTGTACATT CTGGCCGGGC ACGAGGCTAG CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hppy ( NM_001299676.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286605.1 Drosophila melanogaster happyhour (hppy), transcript variant D, mRNA.

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CloneID	OFa21868
Clone ID Related Accession (Same CDS sequence)	NM_001299676.1
Accession Version	NM_001299676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	happyhour, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATAGC GACCTTGCCA CTGAACAACG ACACCAAAGA TCCACTCGGC 
GCCGAGTGCA GCTGCTCCTC CCACAATGGA GGAGCCGCCG GAGGAGGAGG AGGAGGAGGA 
GTTGGAGTAG GAGCAGGCGG AGCAGCCGCG AACGGCAGCA GCAGCAGCAG CGGAGGCGCA 
ACAGTCGGCA CCACTCATCA TCAGCACCAA CAGCACCACC AGCATCACCA CCATCCGAAT 
CATCTGCATC AGCATCAGTC CCATCAATTG CCGCAACAGC AGCAGCAGCA GTCACATCAG 
GCGGCACAGC AGGAACACCA TCACCACCAT CCAATGACCT CGTCCATATC CAGCGGCTTG 
GTGACCGCCA ATGCCAACAA CATCAGCTCG TCCGCCTCGC TGGCCTCCAT GCCAGGTCTC 
AGTGCCTACC TGGGCATGCG CTCCCATGTG GTTGGCCACA TGGGTATGGG ATTTGGCATG 
GGTCTCGGCA TGTCCAACCT GCGCACCACC AGCATCGATG CCGACGACGA TGAACTGGTG 
GCTGCCGATG TGGCCATGAA TAATGCTGCT GCCGCCGTTC CTGGGAACGG ATCCGGTGCT 
GGCAACGGTT CCGGATCCGG ATGCTCCGCC TCTGCGGCGC ACTATCTGCG TTACAGCAGC 
AACTACCGAT CCGCCGCCGC GGCTCAGGCA TCGCAGGCGG CACATCCGCA CGTTAACTCC 
AATTCGAATT CGAACAACGG CCATGGCCAT GCGCCGATCA CATCCAGCAT CTCGTCCTCT 
GCGTCATCAG CTTCGTTTTA TAATCGCCTC CTACTCCTCG ATAATTCCAG CGGCGATGCA 
GTCGTAGGCG GTAACGGAGG CGGCAGCAGC AACATAAGCA GCAGCGGAGG AGCATCAAGT 
GGCACCAATG GCCTCCTGGA TAAGCACGAG CTAGATGCTC TTCCCCAAGC GGCAACAAAG 
TCGGCCGGCG ATGGCTTCTC GCATTCCAAT AGCCCAACGG CGAATTCCGG TGCGGGAGCG 
ACCGGATCCA GGGATAACGA TGGACCCTCG TCGAGCAACT CTTCGCATCT CTACCAGAAC 
CTGCTGAGAA GCAGCTCTGG TGAAACGCCA GCGGGATCGA GCTCGGCGGG CAATAACTGT 
GATTACCGGC ATGAGAACAA CCAGAATGGT CTGGAGGACT CGCCTCGACG CCATAGCTCC 
ATGGATCAGC TGATTGGGTT GCTCGAGAAC ATGGGCAAGT CTCCGAGGAC ACGCAGCCTG 
AGCGATGGTG GCACCCAGGA CGATGACGAA GCGGAGAAGG AGGCGCAACC GGATCTGCTG 
AACAACACCC CACCGGTACC ACCGAAGCGC TCCCACAAAC GCCGTCACAC GCCACCTCGA 
CCCATCTCCA ACGGACTGCC GCCCACACCC AAGGTGCACA TGGGCGCCTG CTTCTCCAAG 
ATCTTCAACG GTTGCCCACT GCGCGTCCAC TGCACCGCCT CGTGGATTCA TCCGGAGACG 
CGGGACCAGC ATCTGCTGAT CGGCGCCGAG GAGGGCATCT ACAACCTCAA CATGAACGAA 
CTGCATGACG CGGCCATCGA TCAGTTGTTC CCGCGCCGCA CCACCTGGTT GTATGTCATC 
AAGGATGTGC TCATGAGTTT GTCAGGAAAA TCCTGCCAGC TCTACAGGCA CGACCTGGTG 
GCCCTGCACT CCAAGCAGAC GGTACGCTTC TCGCTGCACA TGAACAAGAT CCCGGAGCGA 
CTGGTGCCGC GCAAGTTCGC TCTCACCACC AAGGTGCCGG ACACAAAGTG CTGCACACAG 
TGCTGTGTCA CACGGAATCC GTATAACGGA TACAAATATC TCTGTGGGGC GACACCCAGC 
GGCATTTTCC TGATGCAGTG GTACGATCCA CTGAACAAGT TTATGCTGCT CAAGCAGTGC 
GAGTGGCCAG CCATCAGCAT TCAGGGTGGT GGTCATGGCT GCGTTCAGAA CGGACATACG 
CCCGTATTCG AGATGATTAT CACGCCGGAG TTGGAGTACC CGATTGTGTG TACGGGTGTG 
CGAAAGGCCA TGAACGGCTG CCTCAAGCTG GAGCTGATCA ACATGAACAG TGCCAGCTGG 
TTCCATTCGG AGGACCTGGA ATATGACGCC ATGGCCACGA TGGTGCCGCG ACGCGACCTG 
CTGAAGGTGG TGCGTGTGCA CCAGGTGGAG AAGGACGCCA TTCTGGTCTG CTACGGCAAT 
CTGATTCAGG TGGTCACCCT GCAGGGCAAT CCCAAGCAGC ACAAGAAGAT GGTATCGCAG 
CTCAACTTTG ATTTCAACGT GGACAGCATT GTTTGTCTAC CGGACAGCGT TTTGGCATTC 
CATAAGCACG GCATGCAGGG AAAGTCGTTG CGCAATGGCG AGGTGACGCA GGAGATCAAG 
GACATGAGTC GCACCTACAG GCTGCTGGGC AGCGATAAGG TTGTGGCCTT GGAGAGCCAG 
CTGCTGAGGA CCGGCTCCCT GGGCAGCGAG GAGGGACACG ATCTGTACAT TCTGGCCGGG 
CACGAGGCTA GCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286605.1
CDS	370..3987
Translation	MAAAHSHHNANMLSSDISRRNPQDEYELIQKIGSGTYGDVYKAKRIQSNELAAIKVIKLEPSDDIQIIQQEIIMMRDCRHPNIIAYYGSYLRRDKLWICMEFCGGGSLQDIYQVTGPLTEVQIAYMCRETLKGLEYLHSMGKMHRDIKGANILLTEYGDVKLADFGVSAQITATINKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFDLHPMRALFLMSKSGFKPPTLNNKDKWSPTFHNFIKTALTKNPKKRPTAERLLQHPFVQCEMSLRVAKELLQKYQSPNPQFYYYLDGDEESVAGVPQRIASKMTSRTNGVPAQNHTLKTGMTTNSTWNERSSSPETLPSDIATLPLNNDTKDPLGAECSCSSHNGGAAGGGGGGGVGVGAGGAAANGSSSSSGGATVGTTHHQHQQHHQHHHHPNHLHQHQSHQLPQQQQQQSHQAAQQEHHHHHPMTSSISSGLVTANANNISSSASLASMPGLSAYLGMRSHVVGHMGMGFGMGLGMSNLRTTSIDADDDELVAADVAMNNAAAAVPGNGSGAGNGSGSGCSASAAHYLRYSSNYRSAAAAQASQAAHPHVNSNSNSNNGHGHAPITSSISSSASSASFYNRLLLLDNSSGDAVVGGNGGGSSNISSSGGASSGTNGLLDKHELDALPQAATKSAGDGFSHSNSPTANSGAGATGSRDNDGPSSSNSSHLYQNLLRSSSGETPAGSSSAGNNCDYRHENNQNGLEDSPRRHSSMDQLIGLLENMGKSPRTRSLSDGGTQDDDEAEKEAQPDLLNNTPPVPPKRSHKRRHTPPRPISNGLPPTPKVHMGACFSKIFNGCPLRVHCTASWIHPETRDQHLLIGAEEGIYNLNMNELHDAAIDQLFPRRTTWLYVIKDVLMSLSGKSCQLYRHDLVALHSKQTVRFSLHMNKIPERLVPRKFALTTKVPDTKCCTQCCVTRNPYNGYKYLCGATPSGIFLMQWYDPLNKFMLLKQCEWPAISIQGGGHGCVQNGHTPVFEMIITPELEYPIVCTGVRKAMNGCLKLELINMNSASWFHSEDLEYDAMATMVPRRDLLKVVRVHQVEKDAILVCYGNLIQVVTLQGNPKQHKKMVSQLNFDFNVDSIVCLPDSVLAFHKHGMQGKSLRNGEVTQEIKDMSRTYRLLGSDKVVALESQLLRTGSLGSEEGHDLYILAGHEASY

Target ORF information:

RefSeq Version	NM_001299676.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster happyhour (hppy), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299676.1

ORF Insert Sequence:

ATGGCCGCCG CCCACAGTCA CCACAATGCC AATATGCTCA GCTCGGACAT CTCTCGCCGA 
AATCCGCAGG ACGAGTACGA ACTCATCCAA AAGATTGGCT CGGGAACCTA CGGCGACGTC 
TATAAGGCGA AACGAATACA GAGCAATGAG CTGGCTGCCA TCAAGGTCAT CAAGCTGGAG 
CCTTCCGACG ATATACAGAT TATCCAGCAG GAGATTATCA TGATGAGGGA CTGCCGCCAT 
CCGAACATCA TCGCCTACTA CGGTTCGTAC CTGCGCAGGG ATAAACTCTG GATCTGCATG 
GAGTTTTGTG GTGGTGGCAG TCTGCAGGAC ATTTACCAAG TGACCGGTCC CCTCACCGAA 
GTCCAGATCG CGTACATGTG CCGAGAGACT CTCAAGGGAC TCGAGTACCT GCACTCCATG 
GGAAAAATGC ACCGGGACAT CAAGGGCGCA AATATCCTGT TGACGGAGTA CGGCGATGTC 
AAGCTGGCGG ATTTCGGCGT TTCAGCTCAG ATCACGGCCA CAATTAACAA ACGCAAGAGC 
TTCATAGGTA CGCCCTACTG GATGGCTCCT GAGGTGGCAG CCGTGGAACG CAAGGGTGGC 
TATAATCAAC TATGTGATAT TTGGGCCTGC GGCATAACCG CAATCGAACT GGCTGAACTG 
CAACCGCCGA TGTTCGATTT GCACCCGATG CGCGCCCTAT TCCTGATGTC GAAGAGCGGC 
TTTAAGCCGC CCACTCTGAA CAACAAGGAC AAGTGGAGCC CCACGTTCCA CAACTTCATC 
AAGACGGCGC TGACGAAGAA TCCGAAGAAG CGACCCACCG CCGAGCGCCT GCTGCAGCAT 
CCCTTCGTCC AGTGCGAGAT GTCCTTGCGG GTGGCCAAGG AGCTGCTGCA GAAGTACCAG 
AGTCCCAACC CGCAGTTCTA CTACTATCTC GATGGCGATG AGGAGTCTGT GGCAGGAGTG 
CCACAACGCA TTGCCAGCAA AATGACGTCA CGCACCAATG GCGTGCCAGC GCAAAATCAC 
ACACTAAAAA CAGGCATGAC GACGAACTCC ACGTGGAATG AGCGATCTTC TAGTCCCGAA 
ACGTTACCCA GTGACATAGC GACCTTGCCA CTGAACAACG ACACCAAAGA TCCACTCGGC 
GCCGAGTGCA GCTGCTCCTC CCACAATGGA GGAGCCGCCG GAGGAGGAGG AGGAGGAGGA 
GTTGGAGTAG GAGCAGGCGG AGCAGCCGCG AACGGCAGCA GCAGCAGCAG CGGAGGCGCA 
ACAGTCGGCA CCACTCATCA TCAGCACCAA CAGCACCACC AGCATCACCA CCATCCGAAT 
CATCTGCATC AGCATCAGTC CCATCAATTG CCGCAACAGC AGCAGCAGCA GTCACATCAG 
GCGGCACAGC AGGAACACCA TCACCACCAT CCAATGACCT CGTCCATATC CAGCGGCTTG 
GTGACCGCCA ATGCCAACAA CATCAGCTCG TCCGCCTCGC TGGCCTCCAT GCCAGGTCTC 
AGTGCCTACC TGGGCATGCG CTCCCATGTG GTTGGCCACA TGGGTATGGG ATTTGGCATG 
GGTCTCGGCA TGTCCAACCT GCGCACCACC AGCATCGATG CCGACGACGA TGAACTGGTG 
GCTGCCGATG TGGCCATGAA TAATGCTGCT GCCGCCGTTC CTGGGAACGG ATCCGGTGCT 
GGCAACGGTT CCGGATCCGG ATGCTCCGCC TCTGCGGCGC ACTATCTGCG TTACAGCAGC 
AACTACCGAT CCGCCGCCGC GGCTCAGGCA TCGCAGGCGG CACATCCGCA CGTTAACTCC 
AATTCGAATT CGAACAACGG CCATGGCCAT GCGCCGATCA CATCCAGCAT CTCGTCCTCT 
GCGTCATCAG CTTCGTTTTA TAATCGCCTC CTACTCCTCG ATAATTCCAG CGGCGATGCA 
GTCGTAGGCG GTAACGGAGG CGGCAGCAGC AACATAAGCA GCAGCGGAGG AGCATCAAGT 
GGCACCAATG GCCTCCTGGA TAAGCACGAG CTAGATGCTC TTCCCCAAGC GGCAACAAAG 
TCGGCCGGCG ATGGCTTCTC GCATTCCAAT AGCCCAACGG CGAATTCCGG TGCGGGAGCG 
ACCGGATCCA GGGATAACGA TGGACCCTCG TCGAGCAACT CTTCGCATCT CTACCAGAAC 
CTGCTGAGAA GCAGCTCTGG TGAAACGCCA GCGGGATCGA GCTCGGCGGG CAATAACTGT 
GATTACCGGC ATGAGAACAA CCAGAATGGT CTGGAGGACT CGCCTCGACG CCATAGCTCC 
ATGGATCAGC TGATTGGGTT GCTCGAGAAC ATGGGCAAGT CTCCGAGGAC ACGCAGCCTG 
AGCGATGGTG GCACCCAGGA CGATGACGAA GCGGAGAAGG AGGCGCAACC GGATCTGCTG 
AACAACACCC CACCGGTACC ACCGAAGCGC TCCCACAAAC GCCGTCACAC GCCACCTCGA 
CCCATCTCCA ACGGACTGCC GCCCACACCC AAGGTGCACA TGGGCGCCTG CTTCTCCAAG 
ATCTTCAACG GTTGCCCACT GCGCGTCCAC TGCACCGCCT CGTGGATTCA TCCGGAGACG 
CGGGACCAGC ATCTGCTGAT CGGCGCCGAG GAGGGCATCT ACAACCTCAA CATGAACGAA 
CTGCATGACG CGGCCATCGA TCAGTTGTTC CCGCGCCGCA CCACCTGGTT GTATGTCATC 
AAGGATGTGC TCATGAGTTT GTCAGGAAAA TCCTGCCAGC TCTACAGGCA CGACCTGGTG 
GCCCTGCACT CCAAGCAGAC GGTACGCTTC TCGCTGCACA TGAACAAGAT CCCGGAGCGA 
CTGGTGCCGC GCAAGTTCGC TCTCACCACC AAGGTGCCGG ACACAAAGTG CTGCACACAG 
TGCTGTGTCA CACGGAATCC GTATAACGGA TACAAATATC TCTGTGGGGC GACACCCAGC 
GGCATTTTCC TGATGCAGTG GTACGATCCA CTGAACAAGT TTATGCTGCT CAAGCAGTGC 
GAGTGGCCAG CCATCAGCAT TCAGGGTGGT GGTCATGGCT GCGTTCAGAA CGGACATACG 
CCCGTATTCG AGATGATTAT CACGCCGGAG TTGGAGTACC CGATTGTGTG TACGGGTGTG 
CGAAAGGCCA TGAACGGCTG CCTCAAGCTG GAGCTGATCA ACATGAACAG TGCCAGCTGG 
TTCCATTCGG AGGACCTGGA ATATGACGCC ATGGCCACGA TGGTGCCGCG ACGCGACCTG 
CTGAAGGTGG TGCGTGTGCA CCAGGTGGAG AAGGACGCCA TTCTGGTCTG CTACGGCAAT 
CTGATTCAGG TGGTCACCCT GCAGGGCAAT CCCAAGCAGC ACAAGAAGAT GGTATCGCAG 
CTCAACTTTG ATTTCAACGT GGACAGCATT GTTTGTCTAC CGGACAGCGT TTTGGCATTC 
CATAAGCACG GCATGCAGGG AAAGTCGTTG CGCAATGGCG AGGTGACGCA GGAGATCAAG 
GACATGAGTC GCACCTACAG GCTGCTGGGC AGCGATAAGG TTGTGGCCTT GGAGAGCCAG 
CTGCTGAGGA CCGGCTCCCT GGGCAGCGAG GAGGGACACG ATCTGTACAT TCTGGCCGGG 
CACGAGGCTA GCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hppy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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