Atg7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg7
Entrez Gene ID	37141
Full Name	Autophagy-related 7
Synonyms	ATG7,CG5489,Dmel\CG5489,atg-7,atg7
General protein information	Preferred Names Autophagy-related 7 Names CG5489 gene product from transcript CG5489-RB Atg7-PA Atg7-PB Autophagy-specific gene 7 CG5489-PA CG5489-PB autophagy-related gene 7 autophagy-related protein 7
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55E2-55E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_137506.4	NP_611350.1	Autophagy-related 7, isoform A
NM_166298.3	NP_725809.1	Autophagy-related 7, isoform B

Pathways from BioSystems

Homologies

	ATG7	XP_001088170.1
Canis lupus familiaris (dog)	ATG7	XP_849849.2
Drosophila melanogaster (fruit fly)	Atg7	NP_611350.1
Caenorhabditis elegans (roundworm)	atg-7	NP_502064.1
Saccharomyces cerevisiae (baker's yeast)	ATG7	NP_012041.1
Magnaporthe oryzae (rice blast fungus)	MGG_07297	XP_003715525.1
Xenopus tropicalis (tropical clawed frog)	atg7	NP_001123394.1
Mus musculus (house mouse)	Atg7	NP_001240646.1
Danio rerio (zebrafish)	atg7	XP_005172819.1
Kluyveromyces lactis	KLLA0A05137g	XP_451226.1
Neurospora crassa	NCU06672	XP_960919.1
Oryza sativa (rice)	Os01g0614900	NP_001043569.1
Pan troglodytes (chimpanzee)	ATG7	XP_003309672.2
Schizosaccharomyces pombe (fission yeast)	atg7	NP_596084.1
Rattus norvegicus (Norway rat)	Atg7	NP_001012097.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010303	XP_318213.4
Eremothecium gossypii	AGOS_AER298C	NP_985155.1
Arabidopsis thaliana (thale cress)	APG7	NP_568652.1
Homo sapiens (human)	ATG7	NP_006386.1
Bos taurus (cattle)	ATG7	NP_001136439.1
Gallus gallus (chicken)	ATG7	NP_001025763.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies show that the TOR signaling pathway and ATG5 and ATG7 are crucial for the regulation of cellular processes and can play various functions in different cell types in the brain.
Title: TOR signaling pathway and autophagy are involved in the regulation of circadian rhythms in behavior and plasticity of L2 interneurons in the brain of Drosophila melanogaster.

Fasting increases survival to cold in Atg7 mutant Drosophila melanogaster.
Title: Fasting increases survival to cold in FOXO, DIF, autophagy mutants and in other genotypes of Drosophila melanogaster.

Atg7 acts downstream of Hsp27 in the regulation of eye morphology, polyglutamine toxicity, and lifespan in Drosophila.
Title: Autophagy-related gene 7 is downstream of heat shock protein 27 in the regulation of eye morphology, polyglutamine toxicity, and lifespan in Drosophila.

Atg7 mutant adults are hypersensitive to starvation and oxidative stress, live shorter, and accumulate ubiquitin- positive aggregates in the brain that lead to a progressive decline of neuronal function and cell death.
Title: Drosophila Atg7: required for stress resistance, longevity and neuronal homeostasis, but not for metamorphosis.

Atg7-/- adults are short-lived, hypersensitive to nutrient and oxidative stress, and accumulate ubiquitin-positive aggregates in degenerating neurons.
Title: Atg7-dependent autophagy promotes neuronal health, stress tolerance, and longevity but is dispensable for metamorphosis in Drosophila.

The following Atg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10510	NM_166298.3 Latest version!	Drosophila melanogaster Autophagy-related 7 (Atg7), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10511	NM_137506.4 Latest version!	Drosophila melanogaster Autophagy-related 7 (Atg7), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg7 ( NM_166298.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725809.1 Drosophila melanogaster Autophagy-related 7 (Atg7), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10510
Clone ID Related Accession (Same CDS sequence)	NM_166298.3
Accession Version	NM_166298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 7, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166298.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACGG AAAAAGAGAT AATTTTGCAA TTTGCTCCGT GGGAATCTTT TGTGTCGCCC 
ACTTTCTGGC ACAAGCTAGC GGAGCTTAAG CTCGACCACG ATCGCCTGTC CGACTCGAAA 
CGCTCCATTA CTGGACACTA CACAAATCGT AATGCAAGTG GATGCCTTTT GGAAGTAGAC 
TACACGGCCT ACAACAGTCC CTCTTTTGTT GGAAAACCAT TGAAGTTTCT TGGCCTGCGA 
CACAATCAAC AAATGAACAT CGATGACAGC CTTGTATGGA AGGTGATTCA GACTGAAGCT 
TGCGATCTTA GCCAAAGCGA AAATATAAAG TTCGTGGGCT GGGAGCTAAA TAAAAATGGC 
AAAATGGGGC CAAGAATGGT GTGCATGAGG GACAGCATGG ATCCAGCCAA ACTCGCTGAG 
AACTCTGTAA ACCTGAACCT GAAACTAATG AAGTGGCGCC TTGTTCCCGA TCTCAATCTC 
GAAATCATCT CCCAAACAAA ATGCCTGCTC TTTGGAGCTG GTACTTTAGG ATGCGCTGTA 
GCTAGAAATC TGCTGTCCTG GGGATTTAAG CACATAACCC TTTTGGACAG CGGCAAGGTT 
GGGTTCTCTA ATCCAGTGCG TCAGAATCTC TACACGCATG CAGATGCTGT GGCAGGCAAT 
CGGATGAAGG CGACTACAGC TGCTCAGAGA TTGAAAGAGA TCAATCCGTC TGCGGAAACG 
GCGGGTTATG TGCTGGAGAT TCCCATGCCT GGGCATACTA TTGGAGAATC GCTTCTAGCC 
CAGACGAAGG AGCATTTGAA GGTTATCGAA AAGCTTGTGC AGGACCATGA TGTCATCTTT 
CTGCTCACAG ATTCGCGCGA AAGCCGCTGG CTGCCCACCC TGCTTGGAGC GGCAAAGGAA 
AAAATTGTCA TCAACGCAGC TTTAGGCTTT GACAGTTATC TGGTAATGCG ACATGGCACT 
ACGCGTAAGG AAGCAGGCGA CGATGGCCAA GAAATCGAAG GACTTAAGTG CATTAATGGC 
GATCAATTAG GTTGCTACTT TTGTAACGAT GTTACGGCCC CTGGAAATTC GCTCAAAGAC 
CGAACTTTGG ATCAACAGTG CACAGTGACA CGACCCGGAG TATCCAACAT TGCGGCCAGC 
TATGCGGTGG AATTGTTGGT GGCTTTGCTG CAGCATCCTC GTAAGGAGCT GGCACCCGCC 
TACTATGCAC AGAGTGGGCG TGGCAGGTCG GAGGAGACGG AAGAAAAGGT GCCGGAGGGT 
CTGCTAGGAA TTTTGCCTCA CTCCATCCGT GGAATGCTGT GCAACTACGA GAATATTCTG 
CCCGCCACTC AAAAGTTTGC TCAGTGCATA GCCTGTTCAG CGGCCGTCCT AAATGAATAT 
AAAAAAGAGG GTCATGCTTT CCTGTTTAAA ACTTTTGAAA CAGCCAAGTT TCTGGAGGAT 
TTGACGGGAA TCTCCGAATT CAAGCGGTTA AACAGCGAGA TTATCGATTT TGATGATGAA 
GAATTTGACA TGTCCGATAG CGACGAGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725809.1
CDS	86..1618
Translation	MSTEKEIILQFAPWESFVSPTFWHKLAELKLDHDRLSDSKRSITGHYTNRNASGCLLEVDYTAYNSPSFVGKPLKFLGLRHNQQMNIDDSLVWKVIQTEACDLSQSENIKFVGWELNKNGKMGPRMVCMRDSMDPAKLAENSVNLNLKLMKWRLVPDLNLEIISQTKCLLFGAGTLGCAVARNLLSWGFKHITLLDSGKVGFSNPVRQNLYTHADAVAGNRMKATTAAQRLKEINPSAETAGYVLEIPMPGHTIGESLLAQTKEHLKVIEKLVQDHDVIFLLTDSRESRWLPTLLGAAKEKIVINAALGFDSYLVMRHGTTRKEAGDDGQEIEGLKCINGDQLGCYFCNDVTAPGNSLKDRTLDQQCTVTRPGVSNIAASYAVELLVALLQHPRKELAPAYYAQSGRGRSEETEEKVPEGLLGILPHSIRGMLCNYENILPATQKFAQCIACSAAVLNEYKKEGHAFLFKTFETAKFLEDLTGISEFKRLNSEIIDFDDEEFDMSDSDED

Target ORF information:

RefSeq Version	NM_166298.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 7 (Atg7), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166298.3

ORF Insert Sequence:

ATGAGCACGG AAAAAGAGAT AATTTTGCAA TTTGCTCCGT GGGAATCTTT TGTGTCGCCC 
ACTTTCTGGC ACAAGCTAGC GGAGCTTAAG CTCGACCACG ATCGCCTGTC CGACTCGAAA 
CGCTCCATTA CTGGACACTA CACAAATCGT AATGCAAGTG GATGCCTTTT GGAAGTAGAC 
TACACGGCCT ACAACAGTCC CTCTTTTGTT GGAAAACCAT TGAAGTTTCT TGGCCTGCGA 
CACAATCAAC AAATGAACAT CGATGACAGC CTTGTATGGA AGGTGATTCA GACTGAAGCT 
TGCGATCTTA GCCAAAGCGA AAATATAAAG TTCGTGGGCT GGGAGCTAAA TAAAAATGGC 
AAAATGGGGC CAAGAATGGT GTGCATGAGG GACAGCATGG ATCCAGCCAA ACTCGCTGAG 
AACTCTGTAA ACCTGAACCT GAAACTAATG AAGTGGCGCC TTGTTCCCGA TCTCAATCTC 
GAAATCATCT CCCAAACAAA ATGCCTGCTC TTTGGAGCTG GTACTTTAGG ATGCGCTGTA 
GCTAGAAATC TGCTGTCCTG GGGATTTAAG CACATAACCC TTTTGGACAG CGGCAAGGTT 
GGGTTCTCTA ATCCAGTGCG TCAGAATCTC TACACGCATG CAGATGCTGT GGCAGGCAAT 
CGGATGAAGG CGACTACAGC TGCTCAGAGA TTGAAAGAGA TCAATCCGTC TGCGGAAACG 
GCGGGTTATG TGCTGGAGAT TCCCATGCCT GGGCATACTA TTGGAGAATC GCTTCTAGCC 
CAGACGAAGG AGCATTTGAA GGTTATCGAA AAGCTTGTGC AGGACCATGA TGTCATCTTT 
CTGCTCACAG ATTCGCGCGA AAGCCGCTGG CTGCCCACCC TGCTTGGAGC GGCAAAGGAA 
AAAATTGTCA TCAACGCAGC TTTAGGCTTT GACAGTTATC TGGTAATGCG ACATGGCACT 
ACGCGTAAGG AAGCAGGCGA CGATGGCCAA GAAATCGAAG GACTTAAGTG CATTAATGGC 
GATCAATTAG GTTGCTACTT TTGTAACGAT GTTACGGCCC CTGGAAATTC GCTCAAAGAC 
CGAACTTTGG ATCAACAGTG CACAGTGACA CGACCCGGAG TATCCAACAT TGCGGCCAGC 
TATGCGGTGG AATTGTTGGT GGCTTTGCTG CAGCATCCTC GTAAGGAGCT GGCACCCGCC 
TACTATGCAC AGAGTGGGCG TGGCAGGTCG GAGGAGACGG AAGAAAAGGT GCCGGAGGGT 
CTGCTAGGAA TTTTGCCTCA CTCCATCCGT GGAATGCTGT GCAACTACGA GAATATTCTG 
CCCGCCACTC AAAAGTTTGC TCAGTGCATA GCCTGTTCAG CGGCCGTCCT AAATGAATAT 
AAAAAAGAGG GTCATGCTTT CCTGTTTAAA ACTTTTGAAA CAGCCAAGTT TCTGGAGGAT 
TTGACGGGAA TCTCCGAATT CAAGCGGTTA AACAGCGAGA TTATCGATTT TGATGATGAA 
GAATTTGACA TGTCCGATAG CGACGAGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg7 ( NM_137506.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611350.1 Drosophila melanogaster Autophagy-related 7 (Atg7), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10511
Clone ID Related Accession (Same CDS sequence)	NM_137506.4
Accession Version	NM_137506.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 7, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137506.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACGG AAAAAGAGAT AATTTTGCAA TTTGCTCCGT GGGAATCTTT TGTGTCGCCC 
ACTTTCTGGC ACAAGCTAGC GGAGCTTAAG CTCGACCACG ATCGCCTGTC CGACTCGAAA 
CGCTCCATTA CTGGACACTA CACAAATCGT AATGCAAGTG GATGCCTTTT GGAAGTAGAC 
TACACGGCCT ACAACAGAAT GGCAAAGCCT CCAAAATTCA GCCATTCCGC TATAGGCACC 
ATCTACAATA AGAACACAAT CGAAGAGTTC AAGGCCCTGG ACAAATTACA ACTGCTGGCC 
GATGAGGGCA AGGAACTGCT GGCTGATATG TGCAGTGGTG GCGCCTTGAG AGATCCCAGT 
CTATTGACCC GGTTCTTTGT TCTCTCCTTT GCCGATTTAA AGTGTCACAG CTACTACTAT 
TGGTTCGCCT TTCCGTGCCC GCTGACGCCC ACCTTAAAGC TTCAGGGAGC CGTCCAAAAA 
CTGCGGGACT TGCCCAATAG TAGTAGCTAT ATAATGGCTC TAAAGGCCTT ACCCACTGAG 
TCACAGAACT TCTTCATTCT GTATGCTAAT GTGGAGAAGA ACATTTTCGA AGCCCGTAGT 
TTAAGTTCCC TTGACGATAA AAATGTTGAG TTCTGTTATT TTGGTTTTGC TGATCCCAGC 
GAGTATGAGC ATCCAGCTTG GATAATGCGA AACTATGCTG CCTTTCTGCT TCAGCAATGT 
CCCTCTTTTG TTGGAAAACC ATTGAAGTTT CTTGGCCTGC GACACAATCA ACAAATGAAC 
ATCGATGACA GCCTTGTATG GAAGGTGATT CAGACTGAAG CTTGCGATCT TAGCCAAAGC 
GAAAATATAA AGTTCGTGGG CTGGGAGCTA AATAAAAATG GCAAAATGGG GCCAAGAATG 
GTGTGCATGA GGGACAGCAT GGATCCAGCC AAACTCGCTG AGAACTCTGT AAACCTGAAC 
CTGAAACTAA TGAAGTGGCG CCTTGTTCCC GATCTCAATC TCGAAATCAT CTCCCAAACA 
AAATGCCTGC TCTTTGGAGC TGGTACTTTA GGATGCGCTG TAGCTAGAAA TCTGCTGTCC 
TGGGGATTTA AGCACATAAC CCTTTTGGAC AGCGGCAAGG TTGGGTTCTC TAATCCAGTG 
CGTCAGAATC TCTACACGCA TGCAGATGCT GTGGCAGGCA ATCGGATGAA GGCGACTACA 
GCTGCTCAGA GATTGAAAGA GATCAATCCG TCTGCGGAAA CGGCGGGTTA TGTGCTGGAG 
ATTCCCATGC CTGGGCATAC TATTGGAGAA TCGCTTCTAG CCCAGACGAA GGAGCATTTG 
AAGGTTATCG AAAAGCTTGT GCAGGACCAT GATGTCATCT TTCTGCTCAC AGATTCGCGC 
GAAAGCCGCT GGCTGCCCAC CCTGCTTGGA GCGGCAAAGG AAAAAATTGT CATCAACGCA 
GCTTTAGGCT TTGACAGTTA TCTGGTAATG CGACATGGCA CTACGCGTAA GGAAGCAGGC 
GACGATGGCC AAGAAATCGA AGGACTTAAG TGCATTAATG GCGATCAATT AGGTTGCTAC 
TTTTGTAACG ATGTTACGGC CCCTGGAAAT TCGCTCAAAG ACCGAACTTT GGATCAACAG 
TGCACAGTGA CACGACCCGG AGTATCCAAC ATTGCGGCCA GCTATGCGGT GGAATTGTTG 
GTGGCTTTGC TGCAGCATCC TCGTAAGGAG CTGGCACCCG CCTACTATGC ACAGAGTGGG 
CGTGGCAGGT CGGAGGAGAC GGAAGAAAAG GTGCCGGAGG GTCTGCTAGG AATTTTGCCT 
CACTCCATCC GTGGAATGCT GTGCAACTAC GAGAATATTC TGCCCGCCAC TCAAAAGTTT 
GCTCAGTGCA TAGCCTGTTC AGCGGCCGTC CTAAATGAAT ATAAAAAAGA GGGTCATGCT 
TTCCTGTTTA AAACTTTTGA AACAGCCAAG TTTCTGGAGG ATTTGACGGG AATCTCCGAA 
TTCAAGCGGT TAAACAGCGA GATTATCGAT TTTGATGATG AAGAATTTGA CATGTCCGAT 
AGCGACGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611350.1
CDS	86..2140
Translation	MSTEKEIILQFAPWESFVSPTFWHKLAELKLDHDRLSDSKRSITGHYTNRNASGCLLEVDYTAYNRMAKPPKFSHSAIGTIYNKNTIEEFKALDKLQLLADEGKELLADMCSGGALRDPSLLTRFFVLSFADLKCHSYYYWFAFPCPLTPTLKLQGAVQKLRDLPNSSSYIMALKALPTESQNFFILYANVEKNIFEARSLSSLDDKNVEFCYFGFADPSEYEHPAWIMRNYAAFLLQQCPSFVGKPLKFLGLRHNQQMNIDDSLVWKVIQTEACDLSQSENIKFVGWELNKNGKMGPRMVCMRDSMDPAKLAENSVNLNLKLMKWRLVPDLNLEIISQTKCLLFGAGTLGCAVARNLLSWGFKHITLLDSGKVGFSNPVRQNLYTHADAVAGNRMKATTAAQRLKEINPSAETAGYVLEIPMPGHTIGESLLAQTKEHLKVIEKLVQDHDVIFLLTDSRESRWLPTLLGAAKEKIVINAALGFDSYLVMRHGTTRKEAGDDGQEIEGLKCINGDQLGCYFCNDVTAPGNSLKDRTLDQQCTVTRPGVSNIAASYAVELLVALLQHPRKELAPAYYAQSGRGRSEETEEKVPEGLLGILPHSIRGMLCNYENILPATQKFAQCIACSAAVLNEYKKEGHAFLFKTFETAKFLEDLTGISEFKRLNSEIIDFDDEEFDMSDSDED

Target ORF information:

RefSeq Version	NM_137506.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 7 (Atg7), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137506.4

ORF Insert Sequence:

ATGAGCACGG AAAAAGAGAT AATTTTGCAA TTTGCTCCGT GGGAATCTTT TGTGTCGCCC 
ACTTTCTGGC ACAAGCTAGC GGAGCTTAAG CTCGACCACG ATCGCCTGTC CGACTCGAAA 
CGCTCCATTA CTGGACACTA CACAAATCGT AATGCAAGTG GATGCCTTTT GGAAGTAGAC 
TACACGGCCT ACAACAGAAT GGCAAAGCCT CCAAAATTCA GCCATTCCGC TATAGGCACC 
ATCTACAATA AGAACACAAT CGAAGAGTTC AAGGCCCTGG ACAAATTACA ACTGCTGGCC 
GATGAGGGCA AGGAACTGCT GGCTGATATG TGCAGTGGTG GCGCCTTGAG AGATCCCAGT 
CTATTGACCC GGTTCTTTGT TCTCTCCTTT GCCGATTTAA AGTGTCACAG CTACTACTAT 
TGGTTCGCCT TTCCGTGCCC GCTGACGCCC ACCTTAAAGC TTCAGGGAGC CGTCCAAAAA 
CTGCGGGACT TGCCCAATAG TAGTAGCTAT ATAATGGCTC TAAAGGCCTT ACCCACTGAG 
TCACAGAACT TCTTCATTCT GTATGCTAAT GTGGAGAAGA ACATTTTCGA AGCCCGTAGT 
TTAAGTTCCC TTGACGATAA AAATGTTGAG TTCTGTTATT TTGGTTTTGC TGATCCCAGC 
GAGTATGAGC ATCCAGCTTG GATAATGCGA AACTATGCTG CCTTTCTGCT TCAGCAATGT 
CCCTCTTTTG TTGGAAAACC ATTGAAGTTT CTTGGCCTGC GACACAATCA ACAAATGAAC 
ATCGATGACA GCCTTGTATG GAAGGTGATT CAGACTGAAG CTTGCGATCT TAGCCAAAGC 
GAAAATATAA AGTTCGTGGG CTGGGAGCTA AATAAAAATG GCAAAATGGG GCCAAGAATG 
GTGTGCATGA GGGACAGCAT GGATCCAGCC AAACTCGCTG AGAACTCTGT AAACCTGAAC 
CTGAAACTAA TGAAGTGGCG CCTTGTTCCC GATCTCAATC TCGAAATCAT CTCCCAAACA 
AAATGCCTGC TCTTTGGAGC TGGTACTTTA GGATGCGCTG TAGCTAGAAA TCTGCTGTCC 
TGGGGATTTA AGCACATAAC CCTTTTGGAC AGCGGCAAGG TTGGGTTCTC TAATCCAGTG 
CGTCAGAATC TCTACACGCA TGCAGATGCT GTGGCAGGCA ATCGGATGAA GGCGACTACA 
GCTGCTCAGA GATTGAAAGA GATCAATCCG TCTGCGGAAA CGGCGGGTTA TGTGCTGGAG 
ATTCCCATGC CTGGGCATAC TATTGGAGAA TCGCTTCTAG CCCAGACGAA GGAGCATTTG 
AAGGTTATCG AAAAGCTTGT GCAGGACCAT GATGTCATCT TTCTGCTCAC AGATTCGCGC 
GAAAGCCGCT GGCTGCCCAC CCTGCTTGGA GCGGCAAAGG AAAAAATTGT CATCAACGCA 
GCTTTAGGCT TTGACAGTTA TCTGGTAATG CGACATGGCA CTACGCGTAA GGAAGCAGGC 
GACGATGGCC AAGAAATCGA AGGACTTAAG TGCATTAATG GCGATCAATT AGGTTGCTAC 
TTTTGTAACG ATGTTACGGC CCCTGGAAAT TCGCTCAAAG ACCGAACTTT GGATCAACAG 
TGCACAGTGA CACGACCCGG AGTATCCAAC ATTGCGGCCA GCTATGCGGT GGAATTGTTG 
GTGGCTTTGC TGCAGCATCC TCGTAAGGAG CTGGCACCCG CCTACTATGC ACAGAGTGGG 
CGTGGCAGGT CGGAGGAGAC GGAAGAAAAG GTGCCGGAGG GTCTGCTAGG AATTTTGCCT 
CACTCCATCC GTGGAATGCT GTGCAACTAC GAGAATATTC TGCCCGCCAC TCAAAAGTTT 
GCTCAGTGCA TAGCCTGTTC AGCGGCCGTC CTAAATGAAT ATAAAAAAGA GGGTCATGCT 
TTCCTGTTTA AAACTTTTGA AACAGCCAAG TTTCTGGAGG ATTTGACGGG AATCTCCGAA 
TTCAAGCGGT TAAACAGCGA GATTATCGAT TTTGATGATG AAGAATTTGA CATGTCCGAT 
AGCGACGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Atg7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報