pAbp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pAbp
Entrez Gene ID	37070
Full Name	poly(A) binding protein
Synonyms	BcDNA:LD24412,CG16803,CG5119,Dmel\CG5119,PABP,PABP55B,PABPC1,PAbp,Pabp,anon-EST:Posey247,anon-EST:Posey249,anon-EST:fe2E5,dPABP,duo,l(2)SH1908,l(2)SH2 1908,l(2)k10109,pAB,pABP,pabp
General protein information	Preferred Names poly(A) binding protein Names CG5119 gene product from transcript CG5119-RC CG5119-PA CG5119-PB CG5119-PC Poly(A) binding protein Poly(A)-binding protein Poly(A)binding protein PolyA-binding protein anon-fast-evolving-2E5 doppio fuso lethal (2) SH1908 pAbp-PA pAbp-PB pAbp-PC poly (A)-binding protein poly-A binding protein polyadenylate-binding protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55B5-55B9

mRNA and Protein(s)

mRNA	Protein	Name
NM_166265.2	NP_725750.1	poly(A) binding protein, isoform C
NM_166264.4	NP_725749.1	poly(A) binding protein, isoform B
NM_057319.5	NP_476667.1	poly(A) binding protein, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pabpc4	NP_958453.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011092	XP_309558.3
Arabidopsis thaliana (thale cress)	PAB5	NP_177322.2
Homo sapiens (human)	PABPC4	NP_001129125.1
Pan troglodytes (chimpanzee)	PABPC4	XP_513344.3
Mus musculus (house mouse)	Pabpc4	NP_570951.2
Rattus norvegicus (Norway rat)	Pabpc4	NP_001094008.1
Drosophila melanogaster (fruit fly)	pAbp	NP_725749.1
	PABPC4	XP_001113342.2
Gallus gallus (chicken)	PABPC4	XP_004947928.1
Canis lupus familiaris (dog)	PABPC4	XP_005628959.1
Bos taurus (cattle)	PABPC4	NP_001077193.1
Arabidopsis thaliana (thale cress)	PAB3	NP_173690.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PABP association with mRNA rapidly diminished with miRNA-induced silencing complex recruitment and before detectable deadenylation.
Title: PABP and the poly(A) tail augment microRNA repression by facilitated miRISC binding.

Single-molecule nanopore force spectroscopy was used to directly measure interactions between poly(A) and poly(A) binding protein.
Title: Nanopore detachment kinetics of poly(A) binding proteins from RNA molecules reveals the critical role of C-terminus interactions.

PABP generally modulates poly(A) length and translation efficiency, neither PABP function nor GW182-PABP interaction is a prerequisite for these two silencing pathways.
Title: PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro.

Pabp binds to A-rich sequences (ARS) in the osk 3'UTR and these turned out to be required in vivo for osk function during early oogenesis.
Title: Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation.

These findings reveal that despite species-specific differences in the relative strength of the PABPC1-binding sites, the interaction between GW182 proteins and PABPC1 is critical for miRNA-mediated silencing in animal cells.
Title: Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated gene silencing.

The following pAbp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pAbp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10438	NM_057319.5 Latest version!	Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10438	NM_166265.2 Latest version!	Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10438	NM_166264.4 Latest version!	Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pAbp ( NM_166265.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725750.1 Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10438
Clone ID Related Accession (Same CDS sequence)	NM_166265.2 , NM_166264.4 , NM_057319.5
Accession Version	NM_166265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	poly(A) binding protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_166265.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTCTC TATACGTCGG TGATCTCCCA CAGGACGTCA ACGAATCGGG ACTTTTTGAC 
AAGTTCTCGT CTGCTGGTCC AGTGCTGTCC ATTCGTGTCT GCCGCGATGT GATTACCCGT 
CGCTCGTTGG GCTATGCCTA CGTCAACTTC CAGCAGCCAG CCGATGCTGA GCGTGCTTTG 
GACACCATGA ACTTTGACCT GGTTCGCAAC AAGCCCATTC GCATTATGTG GTCTCAGCGT 
GATCCTTCTC TTCGTCGCTC AGGTGTGGGC AACGTGTTCA TCAAGAACTT AGATAGGGCC 
ATCGACAACA AGGCAATCTA CGACACTTTC TCCGCCTTCG GCAACATTTT AAGCTGCAAG 
GTAGCCACCG ATGAAAAGGG CAACTCAAAG GGCTATGGAT TCGTCCACTT CGAGACCGAG 
GAGGCCGCAA ACACGTCCAT CGACAAAGTC AATGGCATGT TGCTCAACGG CAAGAAGGTC 
TACGTGGGTA AGTTCATCCC GCGCAAGGAG CGCGAGAAGG AGCTGGGCGA GAAGGCTAAG 
CTCTTCACCA ATGTATATGT GAAGAACTTC ACTGAGGATT TCGACGATGA AAAACTGAAG 
GAATTCTTTG AGCCCTACGG CAAGATAACC AGCTACAAGG TCATGTCCAA GGAGGATGGC 
AAGAGCAAGG GCTTCGGATT CGTTGCATTC GAGACAACAG AGGCTGCTGA GGCGGCCGTT 
CAGGCTCTCA ATGGCAAGGA CATGGGAGAG GGCAAGTCCC TGTACGTGGC TCGTGCCCAG 
AAGAAGGCCG AACGCCAGCA GGAGCTGAAG CGCAAGTTCG AGGAACTGAA GCAGAAGCGT 
CACGAGTCTG TGTTCGGCGT TAACTTGTAC GTTAAGAACC TGGACGACAC AATCGATGAC 
GATCGTCTGC GCATCGCGTT CTCTCCATAC GGCAACATCA CATCGGCCAA GGTCATGACC 
GATGAGGAGG GTCGCTCCAA GGGATTCGGC TTCGTGTGCT TCAATGCCGC AAGCGAGGCT 
ACCTGCGCCG TCACCGAGCT GAACGGTCGC GTCGTCGGCA GTAAGCCGTT GTACGTTGCT 
TTGGCCCAAA GGAAGGAGGA GCGCAAGGCT CATCTCGCCT CGCAGTACAT GCGTCACATG 
ACCGGCATGC GTATGCAGCA GCTGGGACAG ATCTACCAGC CCAACGCGGC TAGCGGCTTC 
TTTGTGCCGA CCCTTCCGTC AAATCAGCGT TTCTTCGGTT CCCAGGTGGC CACTCAGATG 
AGAAACACAC CCCGCTGGGT GCCCCAGGTG CGTCCCCCAG CAGCCATACA GGGTGTCCAG 
GCCGGAGCTG CCGCTGCTGG TGGCTTCCAG GGCACAGCCG GTGCGGTGCC CACTCAGTTC 
CGTTCGGCTG CTGCTGGCGC TCGCGGGGCA CAGCCTCAGG TGCAGGGCAC ACATGCCGCC 
GCTGCTGCGG CTAACAACAT GCGCAACACT GGAGCACGCG CTATTACTGG CCAGCAAACT 
GCAGCACCCA ACATGCAGAT CCCCGGAGCC CAAATCGCTG GCGGTGCCCA GCAGCGTACT 
TCCAACTATA AGTACACCTC AAACATGCGC AATCCTCCAG TGCCACAGTT GCATCAGACC 
CAGCCAATTC CACAGCAATT GCAAGGAAAG AATTCTGAGA AGCTCATTGC CTCGTTGCTG 
GCCAACGCCA AGCCGCAGGA GCAGAAACAG ATCCTGGGCG AGCGTCTGTA TCCGATGATT 
GAGCATATGC ACGCCAATTT GGCTGGTAAG ATCACCGGCA TGTTGTTGGA GATCGAAAAC 
TCTGAGCTCT TGCACATGAT CGAGGATCAG GAGGCCCTTA AGGCCAAGGT GGAGGAGGCT 
GTGGCCGTGC TTCAGGTGCA CCGCGTCACC GAGCCCGCCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725750.1
CDS	275..2179
Translation	MASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRSGVGNVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEERKAHLASQYMRHMTGMRMQQLGQIYQPNAASGFFVPTLPSNQRFFGSQVATQMRNTPRWVPQVRPPAAIQGVQAGAAAAGGFQGTAGAVPTQFRSAAAGARGAQPQVQGTHAAAAAANNMRNTGARAITGQQTAAPNMQIPGAQIAGGAQQRTSNYKYTSNMRNPPVPQLHQTQPIPQQLQGKNSEKLIASLLANAKPQEQKQILGERLYPMIEHMHANLAGKITGMLLEIENSELLHMIEDQEALKAKVEEAVAVLQVHRVTEPAN

Target ORF information:

RefSeq Version	NM_166265.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166265.2

ORF Insert Sequence:

ATGGCTTCTC TATACGTCGG TGATCTCCCA CAGGACGTCA ACGAATCGGG ACTTTTTGAC 
AAGTTCTCGT CTGCTGGTCC AGTGCTGTCC ATTCGTGTCT GCCGCGATGT GATTACCCGT 
CGCTCGTTGG GCTATGCCTA CGTCAACTTC CAGCAGCCAG CCGATGCTGA GCGTGCTTTG 
GACACCATGA ACTTTGACCT GGTTCGCAAC AAGCCCATTC GCATTATGTG GTCTCAGCGT 
GATCCTTCTC TTCGTCGCTC AGGTGTGGGC AACGTGTTCA TCAAGAACTT AGATAGGGCC 
ATCGACAACA AGGCAATCTA CGACACTTTC TCCGCCTTCG GCAACATTTT AAGCTGCAAG 
GTAGCCACCG ATGAAAAGGG CAACTCAAAG GGCTATGGAT TCGTCCACTT CGAGACCGAG 
GAGGCCGCAA ACACGTCCAT CGACAAAGTC AATGGCATGT TGCTCAACGG CAAGAAGGTC 
TACGTGGGTA AGTTCATCCC GCGCAAGGAG CGCGAGAAGG AGCTGGGCGA GAAGGCTAAG 
CTCTTCACCA ATGTATATGT GAAGAACTTC ACTGAGGATT TCGACGATGA AAAACTGAAG 
GAATTCTTTG AGCCCTACGG CAAGATAACC AGCTACAAGG TCATGTCCAA GGAGGATGGC 
AAGAGCAAGG GCTTCGGATT CGTTGCATTC GAGACAACAG AGGCTGCTGA GGCGGCCGTT 
CAGGCTCTCA ATGGCAAGGA CATGGGAGAG GGCAAGTCCC TGTACGTGGC TCGTGCCCAG 
AAGAAGGCCG AACGCCAGCA GGAGCTGAAG CGCAAGTTCG AGGAACTGAA GCAGAAGCGT 
CACGAGTCTG TGTTCGGCGT TAACTTGTAC GTTAAGAACC TGGACGACAC AATCGATGAC 
GATCGTCTGC GCATCGCGTT CTCTCCATAC GGCAACATCA CATCGGCCAA GGTCATGACC 
GATGAGGAGG GTCGCTCCAA GGGATTCGGC TTCGTGTGCT TCAATGCCGC AAGCGAGGCT 
ACCTGCGCCG TCACCGAGCT GAACGGTCGC GTCGTCGGCA GTAAGCCGTT GTACGTTGCT 
TTGGCCCAAA GGAAGGAGGA GCGCAAGGCT CATCTCGCCT CGCAGTACAT GCGTCACATG 
ACCGGCATGC GTATGCAGCA GCTGGGACAG ATCTACCAGC CCAACGCGGC TAGCGGCTTC 
TTTGTGCCGA CCCTTCCGTC AAATCAGCGT TTCTTCGGTT CCCAGGTGGC CACTCAGATG 
AGAAACACAC CCCGCTGGGT GCCCCAGGTG CGTCCCCCAG CAGCCATACA GGGTGTCCAG 
GCCGGAGCTG CCGCTGCTGG TGGCTTCCAG GGCACAGCCG GTGCGGTGCC CACTCAGTTC 
CGTTCGGCTG CTGCTGGCGC TCGCGGGGCA CAGCCTCAGG TGCAGGGCAC ACATGCCGCC 
GCTGCTGCGG CTAACAACAT GCGCAACACT GGAGCACGCG CTATTACTGG CCAGCAAACT 
GCAGCACCCA ACATGCAGAT CCCCGGAGCC CAAATCGCTG GCGGTGCCCA GCAGCGTACT 
TCCAACTATA AGTACACCTC AAACATGCGC AATCCTCCAG TGCCACAGTT GCATCAGACC 
CAGCCAATTC CACAGCAATT GCAAGGAAAG AATTCTGAGA AGCTCATTGC CTCGTTGCTG 
GCCAACGCCA AGCCGCAGGA GCAGAAACAG ATCCTGGGCG AGCGTCTGTA TCCGATGATT 
GAGCATATGC ACGCCAATTT GGCTGGTAAG ATCACCGGCA TGTTGTTGGA GATCGAAAAC 
TCTGAGCTCT TGCACATGAT CGAGGATCAG GAGGCCCTTA AGGCCAAGGT GGAGGAGGCT 
GTGGCCGTGC TTCAGGTGCA CCGCGTCACC GAGCCCGCCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pAbp ( NM_166264.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725749.1 Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10438
Clone ID Related Accession (Same CDS sequence)	NM_166265.2 , NM_166264.4 , NM_057319.5
Accession Version	NM_166264.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	poly(A) binding protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166264.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTCTC TATACGTCGG TGATCTCCCA CAGGACGTCA ACGAATCGGG ACTTTTTGAC 
AAGTTCTCGT CTGCTGGTCC AGTGCTGTCC ATTCGTGTCT GCCGCGATGT GATTACCCGT 
CGCTCGTTGG GCTATGCCTA CGTCAACTTC CAGCAGCCAG CCGATGCTGA GCGTGCTTTG 
GACACCATGA ACTTTGACCT GGTTCGCAAC AAGCCCATTC GCATTATGTG GTCTCAGCGT 
GATCCTTCTC TTCGTCGCTC AGGTGTGGGC AACGTGTTCA TCAAGAACTT AGATAGGGCC 
ATCGACAACA AGGCAATCTA CGACACTTTC TCCGCCTTCG GCAACATTTT AAGCTGCAAG 
GTAGCCACCG ATGAAAAGGG CAACTCAAAG GGCTATGGAT TCGTCCACTT CGAGACCGAG 
GAGGCCGCAA ACACGTCCAT CGACAAAGTC AATGGCATGT TGCTCAACGG CAAGAAGGTC 
TACGTGGGTA AGTTCATCCC GCGCAAGGAG CGCGAGAAGG AGCTGGGCGA GAAGGCTAAG 
CTCTTCACCA ATGTATATGT GAAGAACTTC ACTGAGGATT TCGACGATGA AAAACTGAAG 
GAATTCTTTG AGCCCTACGG CAAGATAACC AGCTACAAGG TCATGTCCAA GGAGGATGGC 
AAGAGCAAGG GCTTCGGATT CGTTGCATTC GAGACAACAG AGGCTGCTGA GGCGGCCGTT 
CAGGCTCTCA ATGGCAAGGA CATGGGAGAG GGCAAGTCCC TGTACGTGGC TCGTGCCCAG 
AAGAAGGCCG AACGCCAGCA GGAGCTGAAG CGCAAGTTCG AGGAACTGAA GCAGAAGCGT 
CACGAGTCTG TGTTCGGCGT TAACTTGTAC GTTAAGAACC TGGACGACAC AATCGATGAC 
GATCGTCTGC GCATCGCGTT CTCTCCATAC GGCAACATCA CATCGGCCAA GGTCATGACC 
GATGAGGAGG GTCGCTCCAA GGGATTCGGC TTCGTGTGCT TCAATGCCGC AAGCGAGGCT 
ACCTGCGCCG TCACCGAGCT GAACGGTCGC GTCGTCGGCA GTAAGCCGTT GTACGTTGCT 
TTGGCCCAAA GGAAGGAGGA GCGCAAGGCT CATCTCGCCT CGCAGTACAT GCGTCACATG 
ACCGGCATGC GTATGCAGCA GCTGGGACAG ATCTACCAGC CCAACGCGGC TAGCGGCTTC 
TTTGTGCCGA CCCTTCCGTC AAATCAGCGT TTCTTCGGTT CCCAGGTGGC CACTCAGATG 
AGAAACACAC CCCGCTGGGT GCCCCAGGTG CGTCCCCCAG CAGCCATACA GGGTGTCCAG 
GCCGGAGCTG CCGCTGCTGG TGGCTTCCAG GGCACAGCCG GTGCGGTGCC CACTCAGTTC 
CGTTCGGCTG CTGCTGGCGC TCGCGGGGCA CAGCCTCAGG TGCAGGGCAC ACATGCCGCC 
GCTGCTGCGG CTAACAACAT GCGCAACACT GGAGCACGCG CTATTACTGG CCAGCAAACT 
GCAGCACCCA ACATGCAGAT CCCCGGAGCC CAAATCGCTG GCGGTGCCCA GCAGCGTACT 
TCCAACTATA AGTACACCTC AAACATGCGC AATCCTCCAG TGCCACAGTT GCATCAGACC 
CAGCCAATTC CACAGCAATT GCAAGGAAAG AATTCTGAGA AGCTCATTGC CTCGTTGCTG 
GCCAACGCCA AGCCGCAGGA GCAGAAACAG ATCCTGGGCG AGCGTCTGTA TCCGATGATT 
GAGCATATGC ACGCCAATTT GGCTGGTAAG ATCACCGGCA TGTTGTTGGA GATCGAAAAC 
TCTGAGCTCT TGCACATGAT CGAGGATCAG GAGGCCCTTA AGGCCAAGGT GGAGGAGGCT 
GTGGCCGTGC TTCAGGTGCA CCGCGTCACC GAGCCCGCCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725749.1
CDS	62..1966
Translation	MASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRSGVGNVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEERKAHLASQYMRHMTGMRMQQLGQIYQPNAASGFFVPTLPSNQRFFGSQVATQMRNTPRWVPQVRPPAAIQGVQAGAAAAGGFQGTAGAVPTQFRSAAAGARGAQPQVQGTHAAAAAANNMRNTGARAITGQQTAAPNMQIPGAQIAGGAQQRTSNYKYTSNMRNPPVPQLHQTQPIPQQLQGKNSEKLIASLLANAKPQEQKQILGERLYPMIEHMHANLAGKITGMLLEIENSELLHMIEDQEALKAKVEEAVAVLQVHRVTEPAN

Target ORF information:

RefSeq Version	NM_166264.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166264.4

ORF Insert Sequence:

ATGGCTTCTC TATACGTCGG TGATCTCCCA CAGGACGTCA ACGAATCGGG ACTTTTTGAC 
AAGTTCTCGT CTGCTGGTCC AGTGCTGTCC ATTCGTGTCT GCCGCGATGT GATTACCCGT 
CGCTCGTTGG GCTATGCCTA CGTCAACTTC CAGCAGCCAG CCGATGCTGA GCGTGCTTTG 
GACACCATGA ACTTTGACCT GGTTCGCAAC AAGCCCATTC GCATTATGTG GTCTCAGCGT 
GATCCTTCTC TTCGTCGCTC AGGTGTGGGC AACGTGTTCA TCAAGAACTT AGATAGGGCC 
ATCGACAACA AGGCAATCTA CGACACTTTC TCCGCCTTCG GCAACATTTT AAGCTGCAAG 
GTAGCCACCG ATGAAAAGGG CAACTCAAAG GGCTATGGAT TCGTCCACTT CGAGACCGAG 
GAGGCCGCAA ACACGTCCAT CGACAAAGTC AATGGCATGT TGCTCAACGG CAAGAAGGTC 
TACGTGGGTA AGTTCATCCC GCGCAAGGAG CGCGAGAAGG AGCTGGGCGA GAAGGCTAAG 
CTCTTCACCA ATGTATATGT GAAGAACTTC ACTGAGGATT TCGACGATGA AAAACTGAAG 
GAATTCTTTG AGCCCTACGG CAAGATAACC AGCTACAAGG TCATGTCCAA GGAGGATGGC 
AAGAGCAAGG GCTTCGGATT CGTTGCATTC GAGACAACAG AGGCTGCTGA GGCGGCCGTT 
CAGGCTCTCA ATGGCAAGGA CATGGGAGAG GGCAAGTCCC TGTACGTGGC TCGTGCCCAG 
AAGAAGGCCG AACGCCAGCA GGAGCTGAAG CGCAAGTTCG AGGAACTGAA GCAGAAGCGT 
CACGAGTCTG TGTTCGGCGT TAACTTGTAC GTTAAGAACC TGGACGACAC AATCGATGAC 
GATCGTCTGC GCATCGCGTT CTCTCCATAC GGCAACATCA CATCGGCCAA GGTCATGACC 
GATGAGGAGG GTCGCTCCAA GGGATTCGGC TTCGTGTGCT TCAATGCCGC AAGCGAGGCT 
ACCTGCGCCG TCACCGAGCT GAACGGTCGC GTCGTCGGCA GTAAGCCGTT GTACGTTGCT 
TTGGCCCAAA GGAAGGAGGA GCGCAAGGCT CATCTCGCCT CGCAGTACAT GCGTCACATG 
ACCGGCATGC GTATGCAGCA GCTGGGACAG ATCTACCAGC CCAACGCGGC TAGCGGCTTC 
TTTGTGCCGA CCCTTCCGTC AAATCAGCGT TTCTTCGGTT CCCAGGTGGC CACTCAGATG 
AGAAACACAC CCCGCTGGGT GCCCCAGGTG CGTCCCCCAG CAGCCATACA GGGTGTCCAG 
GCCGGAGCTG CCGCTGCTGG TGGCTTCCAG GGCACAGCCG GTGCGGTGCC CACTCAGTTC 
CGTTCGGCTG CTGCTGGCGC TCGCGGGGCA CAGCCTCAGG TGCAGGGCAC ACATGCCGCC 
GCTGCTGCGG CTAACAACAT GCGCAACACT GGAGCACGCG CTATTACTGG CCAGCAAACT 
GCAGCACCCA ACATGCAGAT CCCCGGAGCC CAAATCGCTG GCGGTGCCCA GCAGCGTACT 
TCCAACTATA AGTACACCTC AAACATGCGC AATCCTCCAG TGCCACAGTT GCATCAGACC 
CAGCCAATTC CACAGCAATT GCAAGGAAAG AATTCTGAGA AGCTCATTGC CTCGTTGCTG 
GCCAACGCCA AGCCGCAGGA GCAGAAACAG ATCCTGGGCG AGCGTCTGTA TCCGATGATT 
GAGCATATGC ACGCCAATTT GGCTGGTAAG ATCACCGGCA TGTTGTTGGA GATCGAAAAC 
TCTGAGCTCT TGCACATGAT CGAGGATCAG GAGGCCCTTA AGGCCAAGGT GGAGGAGGCT 
GTGGCCGTGC TTCAGGTGCA CCGCGTCACC GAGCCCGCCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pAbp ( NM_057319.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476667.1 Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10438
Clone ID Related Accession (Same CDS sequence)	NM_166265.2 , NM_166264.4 , NM_057319.5
Accession Version	NM_057319.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	poly(A) binding protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057319.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTCTC TATACGTCGG TGATCTCCCA CAGGACGTCA ACGAATCGGG ACTTTTTGAC 
AAGTTCTCGT CTGCTGGTCC AGTGCTGTCC ATTCGTGTCT GCCGCGATGT GATTACCCGT 
CGCTCGTTGG GCTATGCCTA CGTCAACTTC CAGCAGCCAG CCGATGCTGA GCGTGCTTTG 
GACACCATGA ACTTTGACCT GGTTCGCAAC AAGCCCATTC GCATTATGTG GTCTCAGCGT 
GATCCTTCTC TTCGTCGCTC AGGTGTGGGC AACGTGTTCA TCAAGAACTT AGATAGGGCC 
ATCGACAACA AGGCAATCTA CGACACTTTC TCCGCCTTCG GCAACATTTT AAGCTGCAAG 
GTAGCCACCG ATGAAAAGGG CAACTCAAAG GGCTATGGAT TCGTCCACTT CGAGACCGAG 
GAGGCCGCAA ACACGTCCAT CGACAAAGTC AATGGCATGT TGCTCAACGG CAAGAAGGTC 
TACGTGGGTA AGTTCATCCC GCGCAAGGAG CGCGAGAAGG AGCTGGGCGA GAAGGCTAAG 
CTCTTCACCA ATGTATATGT GAAGAACTTC ACTGAGGATT TCGACGATGA AAAACTGAAG 
GAATTCTTTG AGCCCTACGG CAAGATAACC AGCTACAAGG TCATGTCCAA GGAGGATGGC 
AAGAGCAAGG GCTTCGGATT CGTTGCATTC GAGACAACAG AGGCTGCTGA GGCGGCCGTT 
CAGGCTCTCA ATGGCAAGGA CATGGGAGAG GGCAAGTCCC TGTACGTGGC TCGTGCCCAG 
AAGAAGGCCG AACGCCAGCA GGAGCTGAAG CGCAAGTTCG AGGAACTGAA GCAGAAGCGT 
CACGAGTCTG TGTTCGGCGT TAACTTGTAC GTTAAGAACC TGGACGACAC AATCGATGAC 
GATCGTCTGC GCATCGCGTT CTCTCCATAC GGCAACATCA CATCGGCCAA GGTCATGACC 
GATGAGGAGG GTCGCTCCAA GGGATTCGGC TTCGTGTGCT TCAATGCCGC AAGCGAGGCT 
ACCTGCGCCG TCACCGAGCT GAACGGTCGC GTCGTCGGCA GTAAGCCGTT GTACGTTGCT 
TTGGCCCAAA GGAAGGAGGA GCGCAAGGCT CATCTCGCCT CGCAGTACAT GCGTCACATG 
ACCGGCATGC GTATGCAGCA GCTGGGACAG ATCTACCAGC CCAACGCGGC TAGCGGCTTC 
TTTGTGCCGA CCCTTCCGTC AAATCAGCGT TTCTTCGGTT CCCAGGTGGC CACTCAGATG 
AGAAACACAC CCCGCTGGGT GCCCCAGGTG CGTCCCCCAG CAGCCATACA GGGTGTCCAG 
GCCGGAGCTG CCGCTGCTGG TGGCTTCCAG GGCACAGCCG GTGCGGTGCC CACTCAGTTC 
CGTTCGGCTG CTGCTGGCGC TCGCGGGGCA CAGCCTCAGG TGCAGGGCAC ACATGCCGCC 
GCTGCTGCGG CTAACAACAT GCGCAACACT GGAGCACGCG CTATTACTGG CCAGCAAACT 
GCAGCACCCA ACATGCAGAT CCCCGGAGCC CAAATCGCTG GCGGTGCCCA GCAGCGTACT 
TCCAACTATA AGTACACCTC AAACATGCGC AATCCTCCAG TGCCACAGTT GCATCAGACC 
CAGCCAATTC CACAGCAATT GCAAGGAAAG AATTCTGAGA AGCTCATTGC CTCGTTGCTG 
GCCAACGCCA AGCCGCAGGA GCAGAAACAG ATCCTGGGCG AGCGTCTGTA TCCGATGATT 
GAGCATATGC ACGCCAATTT GGCTGGTAAG ATCACCGGCA TGTTGTTGGA GATCGAAAAC 
TCTGAGCTCT TGCACATGAT CGAGGATCAG GAGGCCCTTA AGGCCAAGGT GGAGGAGGCT 
GTGGCCGTGC TTCAGGTGCA CCGCGTCACC GAGCCCGCCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476667.1
CDS	198..2102
Translation	MASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRSGVGNVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEERKAHLASQYMRHMTGMRMQQLGQIYQPNAASGFFVPTLPSNQRFFGSQVATQMRNTPRWVPQVRPPAAIQGVQAGAAAAGGFQGTAGAVPTQFRSAAAGARGAQPQVQGTHAAAAAANNMRNTGARAITGQQTAAPNMQIPGAQIAGGAQQRTSNYKYTSNMRNPPVPQLHQTQPIPQQLQGKNSEKLIASLLANAKPQEQKQILGERLYPMIEHMHANLAGKITGMLLEIENSELLHMIEDQEALKAKVEEAVAVLQVHRVTEPAN

Target ORF information:

RefSeq Version	NM_057319.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster poly(A) binding protein (pAbp), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057319.5

ORF Insert Sequence:

ATGGCTTCTC TATACGTCGG TGATCTCCCA CAGGACGTCA ACGAATCGGG ACTTTTTGAC 
AAGTTCTCGT CTGCTGGTCC AGTGCTGTCC ATTCGTGTCT GCCGCGATGT GATTACCCGT 
CGCTCGTTGG GCTATGCCTA CGTCAACTTC CAGCAGCCAG CCGATGCTGA GCGTGCTTTG 
GACACCATGA ACTTTGACCT GGTTCGCAAC AAGCCCATTC GCATTATGTG GTCTCAGCGT 
GATCCTTCTC TTCGTCGCTC AGGTGTGGGC AACGTGTTCA TCAAGAACTT AGATAGGGCC 
ATCGACAACA AGGCAATCTA CGACACTTTC TCCGCCTTCG GCAACATTTT AAGCTGCAAG 
GTAGCCACCG ATGAAAAGGG CAACTCAAAG GGCTATGGAT TCGTCCACTT CGAGACCGAG 
GAGGCCGCAA ACACGTCCAT CGACAAAGTC AATGGCATGT TGCTCAACGG CAAGAAGGTC 
TACGTGGGTA AGTTCATCCC GCGCAAGGAG CGCGAGAAGG AGCTGGGCGA GAAGGCTAAG 
CTCTTCACCA ATGTATATGT GAAGAACTTC ACTGAGGATT TCGACGATGA AAAACTGAAG 
GAATTCTTTG AGCCCTACGG CAAGATAACC AGCTACAAGG TCATGTCCAA GGAGGATGGC 
AAGAGCAAGG GCTTCGGATT CGTTGCATTC GAGACAACAG AGGCTGCTGA GGCGGCCGTT 
CAGGCTCTCA ATGGCAAGGA CATGGGAGAG GGCAAGTCCC TGTACGTGGC TCGTGCCCAG 
AAGAAGGCCG AACGCCAGCA GGAGCTGAAG CGCAAGTTCG AGGAACTGAA GCAGAAGCGT 
CACGAGTCTG TGTTCGGCGT TAACTTGTAC GTTAAGAACC TGGACGACAC AATCGATGAC 
GATCGTCTGC GCATCGCGTT CTCTCCATAC GGCAACATCA CATCGGCCAA GGTCATGACC 
GATGAGGAGG GTCGCTCCAA GGGATTCGGC TTCGTGTGCT TCAATGCCGC AAGCGAGGCT 
ACCTGCGCCG TCACCGAGCT GAACGGTCGC GTCGTCGGCA GTAAGCCGTT GTACGTTGCT 
TTGGCCCAAA GGAAGGAGGA GCGCAAGGCT CATCTCGCCT CGCAGTACAT GCGTCACATG 
ACCGGCATGC GTATGCAGCA GCTGGGACAG ATCTACCAGC CCAACGCGGC TAGCGGCTTC 
TTTGTGCCGA CCCTTCCGTC AAATCAGCGT TTCTTCGGTT CCCAGGTGGC CACTCAGATG 
AGAAACACAC CCCGCTGGGT GCCCCAGGTG CGTCCCCCAG CAGCCATACA GGGTGTCCAG 
GCCGGAGCTG CCGCTGCTGG TGGCTTCCAG GGCACAGCCG GTGCGGTGCC CACTCAGTTC 
CGTTCGGCTG CTGCTGGCGC TCGCGGGGCA CAGCCTCAGG TGCAGGGCAC ACATGCCGCC 
GCTGCTGCGG CTAACAACAT GCGCAACACT GGAGCACGCG CTATTACTGG CCAGCAAACT 
GCAGCACCCA ACATGCAGAT CCCCGGAGCC CAAATCGCTG GCGGTGCCCA GCAGCGTACT 
TCCAACTATA AGTACACCTC AAACATGCGC AATCCTCCAG TGCCACAGTT GCATCAGACC 
CAGCCAATTC CACAGCAATT GCAAGGAAAG AATTCTGAGA AGCTCATTGC CTCGTTGCTG 
GCCAACGCCA AGCCGCAGGA GCAGAAACAG ATCCTGGGCG AGCGTCTGTA TCCGATGATT 
GAGCATATGC ACGCCAATTT GGCTGGTAAG ATCACCGGCA TGTTGTTGGA GATCGAAAAC 
TCTGAGCTCT TGCACATGAT CGAGGATCAG GAGGCCCTTA AGGCCAAGGT GGAGGAGGCT 
GTGGCCGTGC TTCAGGTGCA CCGCGTCACC GAGCCCGCCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pAbp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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