Pcl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcl
Entrez Gene ID	37069
Full Name	Polycomblike
Synonyms	CG5109,Dmel\CG5109,PCL,l(2)s1859,pcl
General protein information	Preferred Names Polycomblike Names CG5109 gene product from transcript CG5109-RB CG5109-PA CG5109-PB Pcl-PA Pcl-PB Polycomb-like polycomblike
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55B8-55B8\|2-84 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274138.2	NP_001261067.1	polycomblike, isoform B
NM_057324.6	NP_476672.1	polycomblike, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PHF19	NP_056466.1
Rattus norvegicus (Norway rat)	Phf19	NP_001100040.1
	PHF19	XP_001092389.2
Danio rerio (zebrafish)	phf13	NP_001121998.1
Xenopus tropicalis (tropical clawed frog)	phf19	NP_001072446.1
Gallus gallus (chicken)	PHF19	XP_003642354.2
Bos taurus (cattle)	PHF19	NP_001179644.1
Mus musculus (house mouse)	Phf19	NP_082992.1
Drosophila melanogaster (fruit fly)	Pcl	NP_001261067.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggests that Pcl-Tudor may engage in intra- or intermolecular interactions through an exposed hydrophobic surface patch.
Title: Structure of an atypical Tudor domain in the Drosophila Polycomblike protein.

novel larval complex that contains the distinct polycomb protein Polycomblike ehich binds to the win imaginal discs.
Title: Recruitment of Drosophila Polycomb-group proteins by Polycomblike, a component of a novel protein complex in larvae.

A Pcl-PSC2 complex is the histone methyltransferase that generates the high levels of histone3-lysine27 trimethylation in Polycomb target genes that are needed to maintain a Polycomb-repressed chromatin state.
Title: Pcl-PRC2 is needed to generate high levels of H3-K27 trimethylation at Polycomb target genes.

Consistent with these biochemical and cytological results, Rpd3 mutations enhance the phenotypes of Pcl mutants, further indicating that RPD3 is required for PcG silencing and possibly for PCL function
Title: A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3.

The following Pcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10437	NM_001274138.2 Latest version!	Drosophila melanogaster polycomblike, transcript variant B (Pcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa10437	NM_057324.6 Latest version!	Drosophila melanogaster polycomblike, transcript variant A (Pcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcl ( NM_001274138.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261067.1 Drosophila melanogaster polycomblike, transcript variant B (Pcl), mRNA.

Price & Availability↑

CloneID	OFa10437
Clone ID Related Accession (Same CDS sequence)	NM_001274138.2 , NM_057324.6
Accession Version	NM_001274138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polycomblike, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274138.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAACA ACCATTTTCA CTTGCAACAC GATCATCCAC CGCAGAACGT GGCACATCCG 
TTCATGCAAC AGCCGAGTAC CGCTGTCCCA TCAGCTCCTC CGGCCACCTA CGGCTACTTA 
GCACAGCCAG CTGGCCAGCA GCCGCAGTGG ATGACGACCA CCTACCAGAT CCTGCCGCCC 
AGCGTAGGAC CTGCCACGGT GGCCAAGCGA TACTATGCCA CCACTGGGCC GCAGACCACG 
CATCCCACAC ATCCCAGCAC CATCCAGATC ACGAACAGTT TCGCTCAGCA ATCAACGCCA 
CCGAAGCAAC AGGCGGCAAC AAGCTGCAGC CCATTCAAGG CCAACAACAT ACGAATCATC 
TCGACGGCGC CAAGTGTATA CAGTCTGAAT AAGCCGCCAC AGGAAGCGCA CTCCACATAT 
GCTCCCGTTC AGTCGTACTA CTTGCCCAGT GGCGGTGGCC AAACTGCGGG ACAAATCAAT 
CTACTGGCGG CCAGCGGAAC TGGCAAGCAA CTGCAGCCGC CGCCGCTGGT GCCTGTGACC 
AATTCCACCT CACCGCCCTC CACCGTCGTG TTAGACCGCA TAAACATTTG CATCAATAAC 
CACTATACGG AGACGCCCAC TTCGCTAAGT TCATCGCTTA CCACGGCCCA ACAACCTTCG 
CCCATCATAC CGGCCATCCA GCACAAGGCC ATCCTGCCCC TCATCGACAG CAGCACGGCG 
GATAGCAGCA GCTGCAGCAG CAGTTCTGTA TCCAGCAGCA GTTATAGTGG TACCGCGACC 
ACCTCAGCAG CAGTTGTGAT TGTGGATGAG CCGGATTCTA CGACCACAAC GCCACAGACA 
CCGCCAACTA CGCCGGAGGC CATGAGTTCA CCCGGTAAAA GTTCGCCCTC GCCTCCGCTA 
CTAGCCACGC AATCGCTGCT CAAAGGCGTC AACTCAATGA AGCCAAGCTT CAAGACCGTC 
GAAGCAGCAC CGCCAACGCC GCCAACACCT CCATCACCAC CACCACCGCC GCCGGCACCA 
CCAGTTGCCG CTCCCTCGCC AGCGGTCACG TATGCGCTTC AGGAGGACGT CTTCATCAAA 
TGCAACGATG GCAGATTTTA CCTGGGCACC ATTATTGACC AGACAAGCGA TCAATATCTT 
ATCCGCTTTG ATGACCAGTC GGAGCAATGG TGTGAACCGG ATAAGCTGCG CAAGCTAGGT 
GGCGGTAGCT CGATTACCGC TGGCGGAGGC GGTGCCTCTA CCACGGAATC CACGAATACC 
TCCCCCAGTG GACCCATGTG CGTGGCCTGC AAGCGATCGG ATATCGAAGA TGTGGTCGAA 
ATTTGCGAGC GCTGCGGACG TGGCTACCAT CGTGGTTGCA CCGTTGAAAT TGTTACTGGC 
AGTGGAATTT GGAGTTGCAA ACGCTGCGCT AAACCCATGA AAATGCAACA GCCTGTCAGC 
CATAAGATCA CAAAACCAGC GGGAATTTGT CGCCAGTTGC CCTACCATGC GGATAAGCTC 
AGCTGGGATG AGAAGCATCG GGTCAACGAA GAGCAGATCT ACTGCTACTG CGGCAAACCG 
GGAAAATTCG ATCACAATAT GCTGCAGTGC TGCAAATGTC GGAACTGGTT CCACACCCAA 
TGCATGCAGA ACTTCAAGAA GAAGCTGCTG CGCGGCGATA TGTTCTTTGT TTTCTGCTGC 
ACGGTCTGCA ACAATGGCAT AGAATTCGTT CGTCGTATGC AAATCGAATG GGTGGATGTG 
CTCCACATTG CACTATACAA TCTGCGAAAG CATCAGCACC AGAAGTACCA TCACCTGCTA 
AATGACATTT GGCCCTTCAT ACTCGAGCAG CGCCACCAGC TGCCTATCTG CGAGAAGTGG 
CGCACACTGC CCGAAACGGC ACTGATGGAG CGTCTCAAGC AAACCCTTAA GGATTACTCT 
GACAGATTTG TTTGCGGCAG GGAGTTTAAA AGGGCTCCGG CCTTCTATGC ACTGAGACAC 
AGTGGCCCGC CACACATTCC CAAGGTATTC CTTGAGCCCC ACGAGGAGCT GTCCGATGAG 
CTGCTCGAAA AAAGATTTAA ACTAATGCTC ATGCCCGAGG AGCCTGATGA AGGTGCCAAC 
GAGCTTCCGA AAAGAGTTCC CAAAGATGTG TATGAGTTTA ATACAGATGA AGATGACCCA 
GTCGAGACCA GCGAGGACGA AATACCCATC AAACAGATTA TTGAGAAGGC CAAAAAGCAG 
GCCGCTCAGA AGGCCGATAA GCATGATGAG TTGCCACTGA AGCCGGACCT CGCCGACGAC 
AATGCCAACG ATGGCGATCC CGGCAAACTA CCAGCTCCCA TTCCACCTCT GCTGGACGCA 
AACAGCAGTC GGAAGCGCAA GGCTTTCCGA TTGTCCAAAC GCTATGACAA CAGCCGCAAT 
CACTGTGATC TATCATCCGA CGAGAATTCA AGCAGCAGTC GGGGAACCAG TTCGTTGGAC 
CTCATCATAC CACCGCCGGT CAACTTTTTG GGACGCAACA ATCCATTCCT TATGGCCACT 
CCCAAAAAGG CGTCGCAGGG ACGAAGTATT TCTGTGGGAA CTGGAGTCGG AGTAAATGGC 
ATCATAAACA GCATTTTCAA GCTTAAGGGC ACCTCCAAGG AGCAGCCGCG CATGGTGCGC 
ACCATCAAGC GAAGACTGAG TGCCAAAGAC ATTACGATAG GGCCCAATCA GGAAGTGCGC 
AGACGACGCA CTCGCCGCCT GACCACGGCC ATTGAGGTCA TCAGCACCAC CACCATCAAT 
CCCATACCCA GCCACTACCT TCCTATCTAT GCCAAAGATC TGCAGCCACC CGCGCCGCCA 
ATGGGAAAGC CGACACATGG GCGCCTGTTG CGCCAGCGAC CACAGAAGCA GTCACCCAGC 
CAAAGCCGCC GGAACTCCAC CAGCTCGACG GCAACCAGCA GCAGCAGCAA CGGCATTGGA 
GCCCCAGGAC ATTCCATGCT GGACTTGAAG CAGTCGGTGA ACAAGTACTT TGGTGGCGCT 
ATGAATCGCA TAGATGCTGG TGAGCCCTTT GCCATTCGGG CCAAGCGGCG CATGGGAAAT 
GGACAGGTCC AGTACCTGGT GGAGTGGGGC GGAGATACAG CGACAACGGC GATTGGATTG 
CTTGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261067.1
CDS	499..3630
Translation	MMNNHFHLQHDHPPQNVAHPFMQQPSTAVPSAPPATYGYLAQPAGQQPQWMTTTYQILPPSVGPATVAKRYYATTGPQTTHPTHPSTIQITNSFAQQSTPPKQQAATSCSPFKANNIRIISTAPSVYSLNKPPQEAHSTYAPVQSYYLPSGGGQTAGQINLLAASGTGKQLQPPPLVPVTNSTSPPSTVVLDRINICINNHYTETPTSLSSSLTTAQQPSPIIPAIQHKAILPLIDSSTADSSSCSSSSVSSSSYSGTATTSAAVVIVDEPDSTTTTPQTPPTTPEAMSSPGKSSPSPPLLATQSLLKGVNSMKPSFKTVEAAPPTPPTPPSPPPPPPAPPVAAPSPAVTYALQEDVFIKCNDGRFYLGTIIDQTSDQYLIRFDDQSEQWCEPDKLRKLGGGSSITAGGGGASTTESTNTSPSGPMCVACKRSDIEDVVEICERCGRGYHRGCTVEIVTGSGIWSCKRCAKPMKMQQPVSHKITKPAGICRQLPYHADKLSWDEKHRVNEEQIYCYCGKPGKFDHNMLQCCKCRNWFHTQCMQNFKKKLLRGDMFFVFCCTVCNNGIEFVRRMQIEWVDVLHIALYNLRKHQHQKYHHLLNDIWPFILEQRHQLPICEKWRTLPETALMERLKQTLKDYSDRFVCGREFKRAPAFYALRHSGPPHIPKVFLEPHEELSDELLEKRFKLMLMPEEPDEGANELPKRVPKDVYEFNTDEDDPVETSEDEIPIKQIIEKAKKQAAQKADKHDELPLKPDLADDNANDGDPGKLPAPIPPLLDANSSRKRKAFRLSKRYDNSRNHCDLSSDENSSSSRGTSSLDLIIPPPVNFLGRNNPFLMATPKKASQGRSISVGTGVGVNGIINSIFKLKGTSKEQPRMVRTIKRRLSAKDITIGPNQEVRRRRTRRLTTAIEVISTTTINPIPSHYLPIYAKDLQPPAPPMGKPTHGRLLRQRPQKQSPSQSRRNSTSSTATSSSSNGIGAPGHSMLDLKQSVNKYFGGAMNRIDAGEPFAIRAKRRMGNGQVQYLVEWGGDTATTAIGLLGN

Target ORF information:

RefSeq Version	NM_001274138.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polycomblike, transcript variant B (Pcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274138.2

ORF Insert Sequence:

ATGATGAACA ACCATTTTCA CTTGCAACAC GATCATCCAC CGCAGAACGT GGCACATCCG 
TTCATGCAAC AGCCGAGTAC CGCTGTCCCA TCAGCTCCTC CGGCCACCTA CGGCTACTTA 
GCACAGCCAG CTGGCCAGCA GCCGCAGTGG ATGACGACCA CCTACCAGAT CCTGCCGCCC 
AGCGTAGGAC CTGCCACGGT GGCCAAGCGA TACTATGCCA CCACTGGGCC GCAGACCACG 
CATCCCACAC ATCCCAGCAC CATCCAGATC ACGAACAGTT TCGCTCAGCA ATCAACGCCA 
CCGAAGCAAC AGGCGGCAAC AAGCTGCAGC CCATTCAAGG CCAACAACAT ACGAATCATC 
TCGACGGCGC CAAGTGTATA CAGTCTGAAT AAGCCGCCAC AGGAAGCGCA CTCCACATAT 
GCTCCCGTTC AGTCGTACTA CTTGCCCAGT GGCGGTGGCC AAACTGCGGG ACAAATCAAT 
CTACTGGCGG CCAGCGGAAC TGGCAAGCAA CTGCAGCCGC CGCCGCTGGT GCCTGTGACC 
AATTCCACCT CACCGCCCTC CACCGTCGTG TTAGACCGCA TAAACATTTG CATCAATAAC 
CACTATACGG AGACGCCCAC TTCGCTAAGT TCATCGCTTA CCACGGCCCA ACAACCTTCG 
CCCATCATAC CGGCCATCCA GCACAAGGCC ATCCTGCCCC TCATCGACAG CAGCACGGCG 
GATAGCAGCA GCTGCAGCAG CAGTTCTGTA TCCAGCAGCA GTTATAGTGG TACCGCGACC 
ACCTCAGCAG CAGTTGTGAT TGTGGATGAG CCGGATTCTA CGACCACAAC GCCACAGACA 
CCGCCAACTA CGCCGGAGGC CATGAGTTCA CCCGGTAAAA GTTCGCCCTC GCCTCCGCTA 
CTAGCCACGC AATCGCTGCT CAAAGGCGTC AACTCAATGA AGCCAAGCTT CAAGACCGTC 
GAAGCAGCAC CGCCAACGCC GCCAACACCT CCATCACCAC CACCACCGCC GCCGGCACCA 
CCAGTTGCCG CTCCCTCGCC AGCGGTCACG TATGCGCTTC AGGAGGACGT CTTCATCAAA 
TGCAACGATG GCAGATTTTA CCTGGGCACC ATTATTGACC AGACAAGCGA TCAATATCTT 
ATCCGCTTTG ATGACCAGTC GGAGCAATGG TGTGAACCGG ATAAGCTGCG CAAGCTAGGT 
GGCGGTAGCT CGATTACCGC TGGCGGAGGC GGTGCCTCTA CCACGGAATC CACGAATACC 
TCCCCCAGTG GACCCATGTG CGTGGCCTGC AAGCGATCGG ATATCGAAGA TGTGGTCGAA 
ATTTGCGAGC GCTGCGGACG TGGCTACCAT CGTGGTTGCA CCGTTGAAAT TGTTACTGGC 
AGTGGAATTT GGAGTTGCAA ACGCTGCGCT AAACCCATGA AAATGCAACA GCCTGTCAGC 
CATAAGATCA CAAAACCAGC GGGAATTTGT CGCCAGTTGC CCTACCATGC GGATAAGCTC 
AGCTGGGATG AGAAGCATCG GGTCAACGAA GAGCAGATCT ACTGCTACTG CGGCAAACCG 
GGAAAATTCG ATCACAATAT GCTGCAGTGC TGCAAATGTC GGAACTGGTT CCACACCCAA 
TGCATGCAGA ACTTCAAGAA GAAGCTGCTG CGCGGCGATA TGTTCTTTGT TTTCTGCTGC 
ACGGTCTGCA ACAATGGCAT AGAATTCGTT CGTCGTATGC AAATCGAATG GGTGGATGTG 
CTCCACATTG CACTATACAA TCTGCGAAAG CATCAGCACC AGAAGTACCA TCACCTGCTA 
AATGACATTT GGCCCTTCAT ACTCGAGCAG CGCCACCAGC TGCCTATCTG CGAGAAGTGG 
CGCACACTGC CCGAAACGGC ACTGATGGAG CGTCTCAAGC AAACCCTTAA GGATTACTCT 
GACAGATTTG TTTGCGGCAG GGAGTTTAAA AGGGCTCCGG CCTTCTATGC ACTGAGACAC 
AGTGGCCCGC CACACATTCC CAAGGTATTC CTTGAGCCCC ACGAGGAGCT GTCCGATGAG 
CTGCTCGAAA AAAGATTTAA ACTAATGCTC ATGCCCGAGG AGCCTGATGA AGGTGCCAAC 
GAGCTTCCGA AAAGAGTTCC CAAAGATGTG TATGAGTTTA ATACAGATGA AGATGACCCA 
GTCGAGACCA GCGAGGACGA AATACCCATC AAACAGATTA TTGAGAAGGC CAAAAAGCAG 
GCCGCTCAGA AGGCCGATAA GCATGATGAG TTGCCACTGA AGCCGGACCT CGCCGACGAC 
AATGCCAACG ATGGCGATCC CGGCAAACTA CCAGCTCCCA TTCCACCTCT GCTGGACGCA 
AACAGCAGTC GGAAGCGCAA GGCTTTCCGA TTGTCCAAAC GCTATGACAA CAGCCGCAAT 
CACTGTGATC TATCATCCGA CGAGAATTCA AGCAGCAGTC GGGGAACCAG TTCGTTGGAC 
CTCATCATAC CACCGCCGGT CAACTTTTTG GGACGCAACA ATCCATTCCT TATGGCCACT 
CCCAAAAAGG CGTCGCAGGG ACGAAGTATT TCTGTGGGAA CTGGAGTCGG AGTAAATGGC 
ATCATAAACA GCATTTTCAA GCTTAAGGGC ACCTCCAAGG AGCAGCCGCG CATGGTGCGC 
ACCATCAAGC GAAGACTGAG TGCCAAAGAC ATTACGATAG GGCCCAATCA GGAAGTGCGC 
AGACGACGCA CTCGCCGCCT GACCACGGCC ATTGAGGTCA TCAGCACCAC CACCATCAAT 
CCCATACCCA GCCACTACCT TCCTATCTAT GCCAAAGATC TGCAGCCACC CGCGCCGCCA 
ATGGGAAAGC CGACACATGG GCGCCTGTTG CGCCAGCGAC CACAGAAGCA GTCACCCAGC 
CAAAGCCGCC GGAACTCCAC CAGCTCGACG GCAACCAGCA GCAGCAGCAA CGGCATTGGA 
GCCCCAGGAC ATTCCATGCT GGACTTGAAG CAGTCGGTGA ACAAGTACTT TGGTGGCGCT 
ATGAATCGCA TAGATGCTGG TGAGCCCTTT GCCATTCGGG CCAAGCGGCG CATGGGAAAT 
GGACAGGTCC AGTACCTGGT GGAGTGGGGC GGAGATACAG CGACAACGGC GATTGGATTG 
CTTGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcl ( NM_057324.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476672.1 Drosophila melanogaster polycomblike, transcript variant A (Pcl), mRNA.

Price & Availability↑

CloneID	OFa10437
Clone ID Related Accession (Same CDS sequence)	NM_001274138.2 , NM_057324.6
Accession Version	NM_057324.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polycomblike, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057324.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAACA ACCATTTTCA CTTGCAACAC GATCATCCAC CGCAGAACGT GGCACATCCG 
TTCATGCAAC AGCCGAGTAC CGCTGTCCCA TCAGCTCCTC CGGCCACCTA CGGCTACTTA 
GCACAGCCAG CTGGCCAGCA GCCGCAGTGG ATGACGACCA CCTACCAGAT CCTGCCGCCC 
AGCGTAGGAC CTGCCACGGT GGCCAAGCGA TACTATGCCA CCACTGGGCC GCAGACCACG 
CATCCCACAC ATCCCAGCAC CATCCAGATC ACGAACAGTT TCGCTCAGCA ATCAACGCCA 
CCGAAGCAAC AGGCGGCAAC AAGCTGCAGC CCATTCAAGG CCAACAACAT ACGAATCATC 
TCGACGGCGC CAAGTGTATA CAGTCTGAAT AAGCCGCCAC AGGAAGCGCA CTCCACATAT 
GCTCCCGTTC AGTCGTACTA CTTGCCCAGT GGCGGTGGCC AAACTGCGGG ACAAATCAAT 
CTACTGGCGG CCAGCGGAAC TGGCAAGCAA CTGCAGCCGC CGCCGCTGGT GCCTGTGACC 
AATTCCACCT CACCGCCCTC CACCGTCGTG TTAGACCGCA TAAACATTTG CATCAATAAC 
CACTATACGG AGACGCCCAC TTCGCTAAGT TCATCGCTTA CCACGGCCCA ACAACCTTCG 
CCCATCATAC CGGCCATCCA GCACAAGGCC ATCCTGCCCC TCATCGACAG CAGCACGGCG 
GATAGCAGCA GCTGCAGCAG CAGTTCTGTA TCCAGCAGCA GTTATAGTGG TACCGCGACC 
ACCTCAGCAG CAGTTGTGAT TGTGGATGAG CCGGATTCTA CGACCACAAC GCCACAGACA 
CCGCCAACTA CGCCGGAGGC CATGAGTTCA CCCGGTAAAA GTTCGCCCTC GCCTCCGCTA 
CTAGCCACGC AATCGCTGCT CAAAGGCGTC AACTCAATGA AGCCAAGCTT CAAGACCGTC 
GAAGCAGCAC CGCCAACGCC GCCAACACCT CCATCACCAC CACCACCGCC GCCGGCACCA 
CCAGTTGCCG CTCCCTCGCC AGCGGTCACG TATGCGCTTC AGGAGGACGT CTTCATCAAA 
TGCAACGATG GCAGATTTTA CCTGGGCACC ATTATTGACC AGACAAGCGA TCAATATCTT 
ATCCGCTTTG ATGACCAGTC GGAGCAATGG TGTGAACCGG ATAAGCTGCG CAAGCTAGGT 
GGCGGTAGCT CGATTACCGC TGGCGGAGGC GGTGCCTCTA CCACGGAATC CACGAATACC 
TCCCCCAGTG GACCCATGTG CGTGGCCTGC AAGCGATCGG ATATCGAAGA TGTGGTCGAA 
ATTTGCGAGC GCTGCGGACG TGGCTACCAT CGTGGTTGCA CCGTTGAAAT TGTTACTGGC 
AGTGGAATTT GGAGTTGCAA ACGCTGCGCT AAACCCATGA AAATGCAACA GCCTGTCAGC 
CATAAGATCA CAAAACCAGC GGGAATTTGT CGCCAGTTGC CCTACCATGC GGATAAGCTC 
AGCTGGGATG AGAAGCATCG GGTCAACGAA GAGCAGATCT ACTGCTACTG CGGCAAACCG 
GGAAAATTCG ATCACAATAT GCTGCAGTGC TGCAAATGTC GGAACTGGTT CCACACCCAA 
TGCATGCAGA ACTTCAAGAA GAAGCTGCTG CGCGGCGATA TGTTCTTTGT TTTCTGCTGC 
ACGGTCTGCA ACAATGGCAT AGAATTCGTT CGTCGTATGC AAATCGAATG GGTGGATGTG 
CTCCACATTG CACTATACAA TCTGCGAAAG CATCAGCACC AGAAGTACCA TCACCTGCTA 
AATGACATTT GGCCCTTCAT ACTCGAGCAG CGCCACCAGC TGCCTATCTG CGAGAAGTGG 
CGCACACTGC CCGAAACGGC ACTGATGGAG CGTCTCAAGC AAACCCTTAA GGATTACTCT 
GACAGATTTG TTTGCGGCAG GGAGTTTAAA AGGGCTCCGG CCTTCTATGC ACTGAGACAC 
AGTGGCCCGC CACACATTCC CAAGGTATTC CTTGAGCCCC ACGAGGAGCT GTCCGATGAG 
CTGCTCGAAA AAAGATTTAA ACTAATGCTC ATGCCCGAGG AGCCTGATGA AGGTGCCAAC 
GAGCTTCCGA AAAGAGTTCC CAAAGATGTG TATGAGTTTA ATACAGATGA AGATGACCCA 
GTCGAGACCA GCGAGGACGA AATACCCATC AAACAGATTA TTGAGAAGGC CAAAAAGCAG 
GCCGCTCAGA AGGCCGATAA GCATGATGAG TTGCCACTGA AGCCGGACCT CGCCGACGAC 
AATGCCAACG ATGGCGATCC CGGCAAACTA CCAGCTCCCA TTCCACCTCT GCTGGACGCA 
AACAGCAGTC GGAAGCGCAA GGCTTTCCGA TTGTCCAAAC GCTATGACAA CAGCCGCAAT 
CACTGTGATC TATCATCCGA CGAGAATTCA AGCAGCAGTC GGGGAACCAG TTCGTTGGAC 
CTCATCATAC CACCGCCGGT CAACTTTTTG GGACGCAACA ATCCATTCCT TATGGCCACT 
CCCAAAAAGG CGTCGCAGGG ACGAAGTATT TCTGTGGGAA CTGGAGTCGG AGTAAATGGC 
ATCATAAACA GCATTTTCAA GCTTAAGGGC ACCTCCAAGG AGCAGCCGCG CATGGTGCGC 
ACCATCAAGC GAAGACTGAG TGCCAAAGAC ATTACGATAG GGCCCAATCA GGAAGTGCGC 
AGACGACGCA CTCGCCGCCT GACCACGGCC ATTGAGGTCA TCAGCACCAC CACCATCAAT 
CCCATACCCA GCCACTACCT TCCTATCTAT GCCAAAGATC TGCAGCCACC CGCGCCGCCA 
ATGGGAAAGC CGACACATGG GCGCCTGTTG CGCCAGCGAC CACAGAAGCA GTCACCCAGC 
CAAAGCCGCC GGAACTCCAC CAGCTCGACG GCAACCAGCA GCAGCAGCAA CGGCATTGGA 
GCCCCAGGAC ATTCCATGCT GGACTTGAAG CAGTCGGTGA ACAAGTACTT TGGTGGCGCT 
ATGAATCGCA TAGATGCTGG TGAGCCCTTT GCCATTCGGG CCAAGCGGCG CATGGGAAAT 
GGACAGGTCC AGTACCTGGT GGAGTGGGGC GGAGATACAG CGACAACGGC GATTGGATTG 
CTTGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476672.1
CDS	499..3630
Translation	MMNNHFHLQHDHPPQNVAHPFMQQPSTAVPSAPPATYGYLAQPAGQQPQWMTTTYQILPPSVGPATVAKRYYATTGPQTTHPTHPSTIQITNSFAQQSTPPKQQAATSCSPFKANNIRIISTAPSVYSLNKPPQEAHSTYAPVQSYYLPSGGGQTAGQINLLAASGTGKQLQPPPLVPVTNSTSPPSTVVLDRINICINNHYTETPTSLSSSLTTAQQPSPIIPAIQHKAILPLIDSSTADSSSCSSSSVSSSSYSGTATTSAAVVIVDEPDSTTTTPQTPPTTPEAMSSPGKSSPSPPLLATQSLLKGVNSMKPSFKTVEAAPPTPPTPPSPPPPPPAPPVAAPSPAVTYALQEDVFIKCNDGRFYLGTIIDQTSDQYLIRFDDQSEQWCEPDKLRKLGGGSSITAGGGGASTTESTNTSPSGPMCVACKRSDIEDVVEICERCGRGYHRGCTVEIVTGSGIWSCKRCAKPMKMQQPVSHKITKPAGICRQLPYHADKLSWDEKHRVNEEQIYCYCGKPGKFDHNMLQCCKCRNWFHTQCMQNFKKKLLRGDMFFVFCCTVCNNGIEFVRRMQIEWVDVLHIALYNLRKHQHQKYHHLLNDIWPFILEQRHQLPICEKWRTLPETALMERLKQTLKDYSDRFVCGREFKRAPAFYALRHSGPPHIPKVFLEPHEELSDELLEKRFKLMLMPEEPDEGANELPKRVPKDVYEFNTDEDDPVETSEDEIPIKQIIEKAKKQAAQKADKHDELPLKPDLADDNANDGDPGKLPAPIPPLLDANSSRKRKAFRLSKRYDNSRNHCDLSSDENSSSSRGTSSLDLIIPPPVNFLGRNNPFLMATPKKASQGRSISVGTGVGVNGIINSIFKLKGTSKEQPRMVRTIKRRLSAKDITIGPNQEVRRRRTRRLTTAIEVISTTTINPIPSHYLPIYAKDLQPPAPPMGKPTHGRLLRQRPQKQSPSQSRRNSTSSTATSSSSNGIGAPGHSMLDLKQSVNKYFGGAMNRIDAGEPFAIRAKRRMGNGQVQYLVEWGGDTATTAIGLLGN

Target ORF information:

RefSeq Version	NM_057324.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polycomblike, transcript variant A (Pcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057324.6

ORF Insert Sequence:

ATGATGAACA ACCATTTTCA CTTGCAACAC GATCATCCAC CGCAGAACGT GGCACATCCG 
TTCATGCAAC AGCCGAGTAC CGCTGTCCCA TCAGCTCCTC CGGCCACCTA CGGCTACTTA 
GCACAGCCAG CTGGCCAGCA GCCGCAGTGG ATGACGACCA CCTACCAGAT CCTGCCGCCC 
AGCGTAGGAC CTGCCACGGT GGCCAAGCGA TACTATGCCA CCACTGGGCC GCAGACCACG 
CATCCCACAC ATCCCAGCAC CATCCAGATC ACGAACAGTT TCGCTCAGCA ATCAACGCCA 
CCGAAGCAAC AGGCGGCAAC AAGCTGCAGC CCATTCAAGG CCAACAACAT ACGAATCATC 
TCGACGGCGC CAAGTGTATA CAGTCTGAAT AAGCCGCCAC AGGAAGCGCA CTCCACATAT 
GCTCCCGTTC AGTCGTACTA CTTGCCCAGT GGCGGTGGCC AAACTGCGGG ACAAATCAAT 
CTACTGGCGG CCAGCGGAAC TGGCAAGCAA CTGCAGCCGC CGCCGCTGGT GCCTGTGACC 
AATTCCACCT CACCGCCCTC CACCGTCGTG TTAGACCGCA TAAACATTTG CATCAATAAC 
CACTATACGG AGACGCCCAC TTCGCTAAGT TCATCGCTTA CCACGGCCCA ACAACCTTCG 
CCCATCATAC CGGCCATCCA GCACAAGGCC ATCCTGCCCC TCATCGACAG CAGCACGGCG 
GATAGCAGCA GCTGCAGCAG CAGTTCTGTA TCCAGCAGCA GTTATAGTGG TACCGCGACC 
ACCTCAGCAG CAGTTGTGAT TGTGGATGAG CCGGATTCTA CGACCACAAC GCCACAGACA 
CCGCCAACTA CGCCGGAGGC CATGAGTTCA CCCGGTAAAA GTTCGCCCTC GCCTCCGCTA 
CTAGCCACGC AATCGCTGCT CAAAGGCGTC AACTCAATGA AGCCAAGCTT CAAGACCGTC 
GAAGCAGCAC CGCCAACGCC GCCAACACCT CCATCACCAC CACCACCGCC GCCGGCACCA 
CCAGTTGCCG CTCCCTCGCC AGCGGTCACG TATGCGCTTC AGGAGGACGT CTTCATCAAA 
TGCAACGATG GCAGATTTTA CCTGGGCACC ATTATTGACC AGACAAGCGA TCAATATCTT 
ATCCGCTTTG ATGACCAGTC GGAGCAATGG TGTGAACCGG ATAAGCTGCG CAAGCTAGGT 
GGCGGTAGCT CGATTACCGC TGGCGGAGGC GGTGCCTCTA CCACGGAATC CACGAATACC 
TCCCCCAGTG GACCCATGTG CGTGGCCTGC AAGCGATCGG ATATCGAAGA TGTGGTCGAA 
ATTTGCGAGC GCTGCGGACG TGGCTACCAT CGTGGTTGCA CCGTTGAAAT TGTTACTGGC 
AGTGGAATTT GGAGTTGCAA ACGCTGCGCT AAACCCATGA AAATGCAACA GCCTGTCAGC 
CATAAGATCA CAAAACCAGC GGGAATTTGT CGCCAGTTGC CCTACCATGC GGATAAGCTC 
AGCTGGGATG AGAAGCATCG GGTCAACGAA GAGCAGATCT ACTGCTACTG CGGCAAACCG 
GGAAAATTCG ATCACAATAT GCTGCAGTGC TGCAAATGTC GGAACTGGTT CCACACCCAA 
TGCATGCAGA ACTTCAAGAA GAAGCTGCTG CGCGGCGATA TGTTCTTTGT TTTCTGCTGC 
ACGGTCTGCA ACAATGGCAT AGAATTCGTT CGTCGTATGC AAATCGAATG GGTGGATGTG 
CTCCACATTG CACTATACAA TCTGCGAAAG CATCAGCACC AGAAGTACCA TCACCTGCTA 
AATGACATTT GGCCCTTCAT ACTCGAGCAG CGCCACCAGC TGCCTATCTG CGAGAAGTGG 
CGCACACTGC CCGAAACGGC ACTGATGGAG CGTCTCAAGC AAACCCTTAA GGATTACTCT 
GACAGATTTG TTTGCGGCAG GGAGTTTAAA AGGGCTCCGG CCTTCTATGC ACTGAGACAC 
AGTGGCCCGC CACACATTCC CAAGGTATTC CTTGAGCCCC ACGAGGAGCT GTCCGATGAG 
CTGCTCGAAA AAAGATTTAA ACTAATGCTC ATGCCCGAGG AGCCTGATGA AGGTGCCAAC 
GAGCTTCCGA AAAGAGTTCC CAAAGATGTG TATGAGTTTA ATACAGATGA AGATGACCCA 
GTCGAGACCA GCGAGGACGA AATACCCATC AAACAGATTA TTGAGAAGGC CAAAAAGCAG 
GCCGCTCAGA AGGCCGATAA GCATGATGAG TTGCCACTGA AGCCGGACCT CGCCGACGAC 
AATGCCAACG ATGGCGATCC CGGCAAACTA CCAGCTCCCA TTCCACCTCT GCTGGACGCA 
AACAGCAGTC GGAAGCGCAA GGCTTTCCGA TTGTCCAAAC GCTATGACAA CAGCCGCAAT 
CACTGTGATC TATCATCCGA CGAGAATTCA AGCAGCAGTC GGGGAACCAG TTCGTTGGAC 
CTCATCATAC CACCGCCGGT CAACTTTTTG GGACGCAACA ATCCATTCCT TATGGCCACT 
CCCAAAAAGG CGTCGCAGGG ACGAAGTATT TCTGTGGGAA CTGGAGTCGG AGTAAATGGC 
ATCATAAACA GCATTTTCAA GCTTAAGGGC ACCTCCAAGG AGCAGCCGCG CATGGTGCGC 
ACCATCAAGC GAAGACTGAG TGCCAAAGAC ATTACGATAG GGCCCAATCA GGAAGTGCGC 
AGACGACGCA CTCGCCGCCT GACCACGGCC ATTGAGGTCA TCAGCACCAC CACCATCAAT 
CCCATACCCA GCCACTACCT TCCTATCTAT GCCAAAGATC TGCAGCCACC CGCGCCGCCA 
ATGGGAAAGC CGACACATGG GCGCCTGTTG CGCCAGCGAC CACAGAAGCA GTCACCCAGC 
CAAAGCCGCC GGAACTCCAC CAGCTCGACG GCAACCAGCA GCAGCAGCAA CGGCATTGGA 
GCCCCAGGAC ATTCCATGCT GGACTTGAAG CAGTCGGTGA ACAAGTACTT TGGTGGCGCT 
ATGAATCGCA TAGATGCTGG TGAGCCCTTT GCCATTCGGG CCAAGCGGCG CATGGGAAAT 
GGACAGGTCC AGTACCTGGT GGAGTGGGGC GGAGATACAG CGACAACGGC GATTGGATTG 
CTTGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pcl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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