Sema5c cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema5c
Entrez Gene ID	37066
Full Name	Semaphorin 5c
Synonyms	BEST:CK02567,BG01245,CG5661,CK02567,CT17880,Dmel\CG5661,Dsema3a,Sema 5c,Sema-3a,Sema-5C,Sema-5c,Sema3A,sema 5c,sema-5c
General protein information	Preferred Names Semaphorin 5c Names CG5661 gene product from transcript CG5661-RA CG5661-PA CG5661-PB Sema-3a Sema-5c Sema5c-PA Sema5c-PB Semaphorin-5c semaphorin semaphorin 5C semaphorin 5c semiphorin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68F-68F

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169959.2	NP_001163430.1	semaphorin 5c, isoform B
NM_137449.3	NP_611293.3	semaphorin 5c, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sema5b	NP_001100561.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002424	XP_312521.5
Canis lupus familiaris (dog)	SEMA5B	XP_005639706.1
Bos taurus (cattle)	SEMA5B	XP_002684855.1
Gallus gallus (chicken)	SEMA5B	XP_422096.2
Homo sapiens (human)	SEMA5B	NP_001026872.2
Pan troglodytes (chimpanzee)	SEMA5B	XP_516697.4
Mus musculus (house mouse)	Sema5b	NP_038689.2
	SEMA5B	XP_002802721.1
Danio rerio (zebrafish)	sema5ba	XP_684379.3
Drosophila melanogaster (fruit fly)	Sema-5c	NP_611293.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hypomorphic mutation of this developmental gene results in genomewide transcriptional consequences and alterations in brain structure accompanied by profound impairment of adult behavior.
Title: The early developmental gene Semaphorin 5c contributes to olfactory behavior in adult Drosophila.

The following Sema5c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema5c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17017	NM_137449.3 Latest version!	Drosophila melanogaster semaphorin 5c, transcript variant A (Sema5c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21182	NM_001169959.2 Latest version!	Drosophila melanogaster semaphorin 5c, transcript variant B (Sema5c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema5c ( NM_137449.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611293.3 Drosophila melanogaster semaphorin 5c, transcript variant A (Sema5c), mRNA.

Price & Availability↑

CloneID	OFa17017
Clone ID Related Accession (Same CDS sequence)	NM_137449.3
Accession Version	NM_137449.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	semaphorin 5c, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_137449.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGT TAATACTGAA ACTTCCCAAA ATGTTTAGCC AACTGTGGCT TCTTCTGATT 
CTATCGCTCT TAACCCTAGA AGGCCCACAG CCTTCGACGG GCAGTGGTTA TACGACAAAA 
TCAAGTGTGG ATTTGCTGGA AAACGATTCG CGATACATCT CATATCAAGA TTTAATGTCG 
ACGGCCAAAC GATTTTACGA TCCCGAAACG ACCTGGTATT CGGAAATGCT ATTCGACGTG 
GCCAGGAACC AAGTGATTGT CGGCGCCAGG GACACCCTGT ACCGCATGTC TTTCGACCTG 
GAGCCGCTGG AGCGGGCCAG CTGGGGGGCC ACGCCATCGG AGATCGCCAT GTGCCAGGCA 
AAAGGCCAGT CGGAGCGATG GTGTCGCAAC TATGTGCGCG TGCTCCACAG CTACGGTGAG 
AACCAGCTGT ACGCGTGTGG GACGAACGCC TTCCAGCCGA GCTGTTCGTG GCGCCAGATG 
GAGAACCTCA CAGTCACCGG CGTGGACAGC GGCGTGGTCA AGTGTCCATT TCATCCGCAG 
GCGAACAGCA CCAGTCTGCT GCAGTCCAAT GGGCAACTCT TTGTGGGCAC CGCCACTGAT 
TTCTCGGGCA GCGATGTGGC CATCCTGCGA ACAGGAGTGG AGTCCAATAA GCGTTTCCTC 
CGCACCAAGC AATATAATAA CAACTGGCTG AGTGGAGCCC AATTCGTGGG AAGCTTCGAG 
GCTGGCCACT TTGTATATTT TCTGCTACGC GAATCGGCCG CCGAGCACAT GAGTTGTGGC 
AAGGTGATAT ACTCCAGAGT TGCTAGGGTG TGCAAGAATG ATGTGGGCGG TGGTGGTCAG 
TTGCTAAGGG ACAATTGGAC ATCGTTTCTG AAGGCTCGTC TCAATTGCTC ACTGCCTGGC 
GAATATCCTT ATTATTTCGA TGAGATCCAA GGCATGACGT ATGCCGAATC CGAATCCATT 
CTCTATGCCA CCTTCAGAAC GTCGGGATCC AGCATATTTG GATCAGCTGT TTGTGCCTAC 
AACCTGAGCT CCATTAACGC AGCCTTCGAT GGACCCTTTA AGCAGCAGGA GCACTCGGAT 
GCGGCCTGGA AAACGGTCAA CACGAATCAG CGGAGTCAGT TCCAGTGCGG AACGAGTAGT 
ATTGGCCACT GGCTGGAGAG CTCACGTTAC CAACTGATGG ATGAGGCAGT GCAGCCAATT 
GGAGCAGAGC CATTGTACCA CTCCAAACTG GAACAATTCG GCCGCCTGGC GTTGGACATC 
ATAAACACAA AGACGGAGCA AGTGCACGTG CTCTTCGTGG CCAGCAGTGG GAATCACATA 
AAGAAATTGT CCGTAAAGTA CGATGGCGAT GGAGTTCAGA CATGTCTGGT GGAACTTTGG 
CAGGCGGACG ATACTGGTAC TAGTAGCCTA CTGAATATGG CCTATCTGAA AGTCAGTGAT 
TCCCTCTATC TGGGCACCGA TCTGGCGCTC ACCCGGATTC CCGCCCAGCA CTGCAGTCGC 
CATGTGTCGC AGTCAAGTTG TCTGAACTCC ATGGATCCCT ACTGCGGCTG GAACGAACTG 
GTGGAGCGCT GCATGCCACA GCCACAGGAT TCCTCTGTAC TGCAACACTG GCATCAGGCG 
CCACAGATCA CCTGTCCTGT ACTAAATGCC CCCATCGATG GCGGTTGGTC TACCTGGAGT 
CCATGGGCCG TTTGCCAGCA GCACGAGCAG CCGGATAGCA ATTGCCAGTG CCGACAGCGC 
AGTTGCAACA ATCCTCAGCC ACAACATGGA GGTGCCACCT GCGAGGGCAT CAGCACCCAA 
GTGACGAACT GCACGCAGCA CGGAGGATGG ACGGAGTGGT CAGCATGGTC GCCCTGCTCC 
CAGACCTGCG GCATTGCCGT GAAGATCCGA CGACGCACCT GTGGAAATCC GCGTCCGGCA 
TTCGGCGGTC GCACCTGTGT GGGATCGGAG CAGTCGGAGA TGTACTGCCG CCACTTGCCA 
CCGTGCCCGG TGGCCAAGCC GCAGTCCGTG GATGGCGGAT GGGGACCGTG GGGCGAGTGG 
AGTGAGTGCA GTGCCCAGTG CGGCGGTGGT TTCCGAATGC GACGACGTGA GTGCAACGAT 
CCGGCTCCAC TCAATGGCGG AATGGAGTGC CCGGGCTGTC GGCTGGACTA TGAGGAGTGC 
AACATGCAGA GCTGTCAGGA GGTGCGCAAA CTCAGTGCCT GGACACCGTG GCTAACGGTG 
ACCGGGAGCG GAAATTCAAG TGAACCGCAC ATGGAGAGGC GATTCAGGTT CGTCTGTCGT 
GCCACCAGTC CAGATCCGAG TTCCGTCCGA ATTGGCCTGC CCAAGGAGGA GTCGCGCAAT 
TGTCATGCTG ATGGCAGCTG CCAGCGGCAG GTTGATCACG ACTCCGCGGA CTCCGATGCT 
GCGGACTGGT CACCATGCAG CGTCAGTTGC GGTGGTGGTG TCCAGCAACG TCACCGCGGT 
CGTGGAAGCC AGAGTCGGGT ATGCAATATA CACGCCTGCC CGGCGGAAGA ACAGCTAAGT 
AGTAATAGCC TGGATAACGA GTTGGAACAC GGCGAATGGG GCTGCTGGTC CGAGTGGTCT 
GCCTGCTCGG TGACCTGCGG ATTGGGACTA CGGCGGAGGA CACGTCGTTG CCTAGCGGGT 
CACGACCGGC TATGTCAGGG TCGTGCACTC GAAGAACAGA AGTGTGAAAT GGTGCCATGC 
GAAGATTTCC TAGGTTGGAG CGCTTGGAGC GAGTGGTCGA GCTGCTCCAG CGATGGCATC 
CGACTGAGAC ATCGCCGCTG CCTGGTGGAG CAGCCCGGTT CGATGGAGTG CCGTGGTGCG 
GAGTTCGAGA AGACCGCCTG TGTGCCCAAT GAGTGCGAGG AAACCCAAAC TGCTTCGACT 
GCCACGCTAC CCATCGTGAT CTTCGTTGGC CTGCTTTTCA CAGTGGCCTG TTGCCTGGCC 
ACCTACAGAT TCACCAAGAA ACGATTCATG TTAAGTGCTG AGGAGGCATT AAACAAAACC 
ACGACGACAA CAGCCAGTTT CGACACCTAT CCCAATCAGT ACTCCAGTCT GCCCACCAAG 
GATTACTACG ATCAGCGACC CAAACGGCAG TCCAGCTTCC GGATGCCGGC CAAGACCAGT 
AATTTGGGCA ACGGCAATGG CACCCTCAAT CGGAACAACA TGCACCAAAA TAATACGCCC 
AAAGTTCTGG CCAAAACGTA TATCGACTGC GAATCCGGAA CGATCAAACG ACAATCGGCA 
CTGAACAATT GCCGCAGTAA TATCGACGAC GAGAAGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611293.3
CDS	480..3761
Translation	MNMLILKLPKMFSQLWLLLILSLLTLEGPQPSTGSGYTTKSSVDLLENDSRYISYQDLMSTAKRFYDPETTWYSEMLFDVARNQVIVGARDTLYRMSFDLEPLERASWGATPSEIAMCQAKGQSERWCRNYVRVLHSYGENQLYACGTNAFQPSCSWRQMENLTVTGVDSGVVKCPFHPQANSTSLLQSNGQLFVGTATDFSGSDVAILRTGVESNKRFLRTKQYNNNWLSGAQFVGSFEAGHFVYFLLRESAAEHMSCGKVIYSRVARVCKNDVGGGGQLLRDNWTSFLKARLNCSLPGEYPYYFDEIQGMTYAESESILYATFRTSGSSIFGSAVCAYNLSSINAAFDGPFKQQEHSDAAWKTVNTNQRSQFQCGTSSIGHWLESSRYQLMDEAVQPIGAEPLYHSKLEQFGRLALDIINTKTEQVHVLFVASSGNHIKKLSVKYDGDGVQTCLVELWQADDTGTSSLLNMAYLKVSDSLYLGTDLALTRIPAQHCSRHVSQSSCLNSMDPYCGWNELVERCMPQPQDSSVLQHWHQAPQITCPVLNAPIDGGWSTWSPWAVCQQHEQPDSNCQCRQRSCNNPQPQHGGATCEGISTQVTNCTQHGGWTEWSAWSPCSQTCGIAVKIRRRTCGNPRPAFGGRTCVGSEQSEMYCRHLPPCPVAKPQSVDGGWGPWGEWSECSAQCGGGFRMRRRECNDPAPLNGGMECPGCRLDYEECNMQSCQEVRKLSAWTPWLTVTGSGNSSEPHMERRFRFVCRATSPDPSSVRIGLPKEESRNCHADGSCQRQVDHDSADSDAADWSPCSVSCGGGVQQRHRGRGSQSRVCNIHACPAEEQLSSNSLDNELEHGEWGCWSEWSACSVTCGLGLRRRTRRCLAGHDRLCQGRALEEQKCEMVPCEDFLGWSAWSEWSSCSSDGIRLRHRRCLVEQPGSMECRGAEFEKTACVPNECEETQTASTATLPIVIFVGLLFTVACCLATYRFTKKRFMLSAEEALNKTTTTTASFDTYPNQYSSLPTKDYYDQRPKRQSSFRMPAKTSNLGNGNGTLNRNNMHQNNTPKVLAKTYIDCESGTIKRQSALNNCRSNIDDEKF

Target ORF information:

RefSeq Version	NM_137449.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster semaphorin 5c, transcript variant A (Sema5c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137449.3

ORF Insert Sequence:

ATGAACATGT TAATACTGAA ACTTCCCAAA ATGTTTAGCC AACTGTGGCT TCTTCTGATT 
CTATCGCTCT TAACCCTAGA AGGCCCACAG CCTTCGACGG GCAGTGGTTA TACGACAAAA 
TCAAGTGTGG ATTTGCTGGA AAACGATTCG CGATACATCT CATATCAAGA TTTAATGTCG 
ACGGCCAAAC GATTTTACGA TCCCGAAACG ACCTGGTATT CGGAAATGCT ATTCGACGTG 
GCCAGGAACC AAGTGATTGT CGGCGCCAGG GACACCCTGT ACCGCATGTC TTTCGACCTG 
GAGCCGCTGG AGCGGGCCAG CTGGGGGGCC ACGCCATCGG AGATCGCCAT GTGCCAGGCA 
AAAGGCCAGT CGGAGCGATG GTGTCGCAAC TATGTGCGCG TGCTCCACAG CTACGGTGAG 
AACCAGCTGT ACGCGTGTGG GACGAACGCC TTCCAGCCGA GCTGTTCGTG GCGCCAGATG 
GAGAACCTCA CAGTCACCGG CGTGGACAGC GGCGTGGTCA AGTGTCCATT TCATCCGCAG 
GCGAACAGCA CCAGTCTGCT GCAGTCCAAT GGGCAACTCT TTGTGGGCAC CGCCACTGAT 
TTCTCGGGCA GCGATGTGGC CATCCTGCGA ACAGGAGTGG AGTCCAATAA GCGTTTCCTC 
CGCACCAAGC AATATAATAA CAACTGGCTG AGTGGAGCCC AATTCGTGGG AAGCTTCGAG 
GCTGGCCACT TTGTATATTT TCTGCTACGC GAATCGGCCG CCGAGCACAT GAGTTGTGGC 
AAGGTGATAT ACTCCAGAGT TGCTAGGGTG TGCAAGAATG ATGTGGGCGG TGGTGGTCAG 
TTGCTAAGGG ACAATTGGAC ATCGTTTCTG AAGGCTCGTC TCAATTGCTC ACTGCCTGGC 
GAATATCCTT ATTATTTCGA TGAGATCCAA GGCATGACGT ATGCCGAATC CGAATCCATT 
CTCTATGCCA CCTTCAGAAC GTCGGGATCC AGCATATTTG GATCAGCTGT TTGTGCCTAC 
AACCTGAGCT CCATTAACGC AGCCTTCGAT GGACCCTTTA AGCAGCAGGA GCACTCGGAT 
GCGGCCTGGA AAACGGTCAA CACGAATCAG CGGAGTCAGT TCCAGTGCGG AACGAGTAGT 
ATTGGCCACT GGCTGGAGAG CTCACGTTAC CAACTGATGG ATGAGGCAGT GCAGCCAATT 
GGAGCAGAGC CATTGTACCA CTCCAAACTG GAACAATTCG GCCGCCTGGC GTTGGACATC 
ATAAACACAA AGACGGAGCA AGTGCACGTG CTCTTCGTGG CCAGCAGTGG GAATCACATA 
AAGAAATTGT CCGTAAAGTA CGATGGCGAT GGAGTTCAGA CATGTCTGGT GGAACTTTGG 
CAGGCGGACG ATACTGGTAC TAGTAGCCTA CTGAATATGG CCTATCTGAA AGTCAGTGAT 
TCCCTCTATC TGGGCACCGA TCTGGCGCTC ACCCGGATTC CCGCCCAGCA CTGCAGTCGC 
CATGTGTCGC AGTCAAGTTG TCTGAACTCC ATGGATCCCT ACTGCGGCTG GAACGAACTG 
GTGGAGCGCT GCATGCCACA GCCACAGGAT TCCTCTGTAC TGCAACACTG GCATCAGGCG 
CCACAGATCA CCTGTCCTGT ACTAAATGCC CCCATCGATG GCGGTTGGTC TACCTGGAGT 
CCATGGGCCG TTTGCCAGCA GCACGAGCAG CCGGATAGCA ATTGCCAGTG CCGACAGCGC 
AGTTGCAACA ATCCTCAGCC ACAACATGGA GGTGCCACCT GCGAGGGCAT CAGCACCCAA 
GTGACGAACT GCACGCAGCA CGGAGGATGG ACGGAGTGGT CAGCATGGTC GCCCTGCTCC 
CAGACCTGCG GCATTGCCGT GAAGATCCGA CGACGCACCT GTGGAAATCC GCGTCCGGCA 
TTCGGCGGTC GCACCTGTGT GGGATCGGAG CAGTCGGAGA TGTACTGCCG CCACTTGCCA 
CCGTGCCCGG TGGCCAAGCC GCAGTCCGTG GATGGCGGAT GGGGACCGTG GGGCGAGTGG 
AGTGAGTGCA GTGCCCAGTG CGGCGGTGGT TTCCGAATGC GACGACGTGA GTGCAACGAT 
CCGGCTCCAC TCAATGGCGG AATGGAGTGC CCGGGCTGTC GGCTGGACTA TGAGGAGTGC 
AACATGCAGA GCTGTCAGGA GGTGCGCAAA CTCAGTGCCT GGACACCGTG GCTAACGGTG 
ACCGGGAGCG GAAATTCAAG TGAACCGCAC ATGGAGAGGC GATTCAGGTT CGTCTGTCGT 
GCCACCAGTC CAGATCCGAG TTCCGTCCGA ATTGGCCTGC CCAAGGAGGA GTCGCGCAAT 
TGTCATGCTG ATGGCAGCTG CCAGCGGCAG GTTGATCACG ACTCCGCGGA CTCCGATGCT 
GCGGACTGGT CACCATGCAG CGTCAGTTGC GGTGGTGGTG TCCAGCAACG TCACCGCGGT 
CGTGGAAGCC AGAGTCGGGT ATGCAATATA CACGCCTGCC CGGCGGAAGA ACAGCTAAGT 
AGTAATAGCC TGGATAACGA GTTGGAACAC GGCGAATGGG GCTGCTGGTC CGAGTGGTCT 
GCCTGCTCGG TGACCTGCGG ATTGGGACTA CGGCGGAGGA CACGTCGTTG CCTAGCGGGT 
CACGACCGGC TATGTCAGGG TCGTGCACTC GAAGAACAGA AGTGTGAAAT GGTGCCATGC 
GAAGATTTCC TAGGTTGGAG CGCTTGGAGC GAGTGGTCGA GCTGCTCCAG CGATGGCATC 
CGACTGAGAC ATCGCCGCTG CCTGGTGGAG CAGCCCGGTT CGATGGAGTG CCGTGGTGCG 
GAGTTCGAGA AGACCGCCTG TGTGCCCAAT GAGTGCGAGG AAACCCAAAC TGCTTCGACT 
GCCACGCTAC CCATCGTGAT CTTCGTTGGC CTGCTTTTCA CAGTGGCCTG TTGCCTGGCC 
ACCTACAGAT TCACCAAGAA ACGATTCATG TTAAGTGCTG AGGAGGCATT AAACAAAACC 
ACGACGACAA CAGCCAGTTT CGACACCTAT CCCAATCAGT ACTCCAGTCT GCCCACCAAG 
GATTACTACG ATCAGCGACC CAAACGGCAG TCCAGCTTCC GGATGCCGGC CAAGACCAGT 
AATTTGGGCA ACGGCAATGG CACCCTCAAT CGGAACAACA TGCACCAAAA TAATACGCCC 
AAAGTTCTGG CCAAAACGTA TATCGACTGC GAATCCGGAA CGATCAAACG ACAATCGGCA 
CTGAACAATT GCCGCAGTAA TATCGACGAC GAGAAGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5c ( NM_001169959.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163430.1 Drosophila melanogaster semaphorin 5c, transcript variant B (Sema5c), mRNA.

Price & Availability↑

CloneID	OFa21182
Clone ID Related Accession (Same CDS sequence)	NM_001169959.2
Accession Version	NM_001169959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	semaphorin 5c, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001169959.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAATAC TGAAACTTCC CAAAATGTTT AGCCAACTGT GGCTTCTTCT GATTCTATCG 
CTCTTAACCC TAGAAGGCCC ACAGCCTTCG ACGGGCAGTG GTTATACGAC AAAATCAAGT 
GTGGATTTGC TGGAAAACGA TTCGCGATAC ATCTCATATC AAGATTTAAT GTCGACGGCC 
AAACGATTTT ACGATCCCGA AACGACCTGG TATTCGGAAA TGCTATTCGA CGTGGCCAGG 
AACCAAGTGA TTGTCGGCGC CAGGGACACC CTGTACCGCA TGTCTTTCGA CCTGGAGCCG 
CTGGAGCGGG CCAGCTGGGG GGCCACGCCA TCGGAGATCG CCATGTGCCA GGCAAAAGGC 
CAGTCGGAGC GATGGTGTCG CAACTATGTG CGCGTGCTCC ACAGCTACGG TGAGAACCAG 
CTGTACGCGT GTGGGACGAA CGCCTTCCAG CCGAGCTGTT CGTGGCGCCA GATGGAGAAC 
CTCACAGTCA CCGGCGTGGA CAGCGGCGTG GTCAAGTGTC CATTTCATCC GCAGGCGAAC 
AGCACCAGTC TGCTGCAGTC CAATGGGCAA CTCTTTGTGG GCACCGCCAC TGATTTCTCG 
GGCAGCGATG TGGCCATCCT GCGAACAGGA GTGGAGTCCA ATAAGCGTTT CCTCCGCACC 
AAGCAATATA ATAACAACTG GCTGAGTGGA GCCCAATTCG TGGGAAGCTT CGAGGCTGGC 
CACTTTGTAT ATTTTCTGCT ACGCGAATCG GCCGCCGAGC ACATGAGTTG TGGCAAGGTG 
ATATACTCCA GAGTTGCTAG GGTGTGCAAG AATGATGTGG GCGGTGGTGG TCAGTTGCTA 
AGGGACAATT GGACATCGTT TCTGAAGGCT CGTCTCAATT GCTCACTGCC TGGCGAATAT 
CCTTATTATT TCGATGAGAT CCAAGGCATG ACGTATGCCG AATCCGAATC CATTCTCTAT 
GCCACCTTCA GAACGTCGGG ATCCAGCATA TTTGGATCAG CTGTTTGTGC CTACAACCTG 
AGCTCCATTA ACGCAGCCTT CGATGGACCC TTTAAGCAGC AGGAGCACTC GGATGCGGCC 
TGGAAAACGG TCAACACGAA TCAGCGGAGT CAGTTCCAGT GCGGAACGAG TAGTATTGGC 
CACTGGCTGG AGAGCTCACG TTACCAACTG ATGGATGAGG CAGTGCAGCC AATTGGAGCA 
GAGCCATTGT ACCACTCCAA ACTGGAACAA TTCGGCCGCC TGGCGTTGGA CATCATAAAC 
ACAAAGACGG AGCAAGTGCA CGTGCTCTTC GTGGCCAGCA GTGGGAATCA CATAAAGAAA 
TTGTCCGTAA AGTACGATGG CGATGGAGTT CAGACATGTC TGGTGGAACT TTGGCAGGCG 
GACGATACTG GTACTAGTAG CCTACTGAAT ATGGCCTATC TGAAAGTCAG TGATTCCCTC 
TATCTGGGCA CCGATCTGGC GCTCACCCGG ATTCCCGCCC AGCACTGCAG TCGCCATGTG 
TCGCAGTCAA GTTGTCTGAA CTCCATGGAT CCCTACTGCG GCTGGAACGA ACTGGTGGAG 
CGCTGCATGC CACAGCCACA GGATTCCTCT GTACTGCAAC ACTGGCATCA GGCGCCACAG 
ATCACCTGTC CTGTACTAAA TGCCCCCATC GATGGCGGTT GGTCTACCTG GAGTCCATGG 
GCCGTTTGCC AGCAGCACGA GCAGCCGGAT AGCAATTGCC AGTGCCGACA GCGCAGTTGC 
AACAATCCTC AGCCACAACA TGGAGGTGCC ACCTGCGAGG GCATCAGCAC CCAAGTGACG 
AACTGCACGC AGCACGGAGG ATGGACGGAG TGGTCAGCAT GGTCGCCCTG CTCCCAGACC 
TGCGGCATTG CCGTGAAGAT CCGACGACGC ACCTGTGGAA ATCCGCGTCC GGCATTCGGC 
GGTCGCACCT GTGTGGGATC GGAGCAGTCG GAGATGTACT GCCGCCACTT GCCACCGTGC 
CCGGTGGCCA AGCCGCAGTC CGTGGATGGC GGATGGGGAC CGTGGGGCGA GTGGAGTGAG 
TGCAGTGCCC AGTGCGGCGG TGGTTTCCGA ATGCGACGAC GTGAGTGCAA CGATCCGGCT 
CCACTCAATG GCGGAATGGA GTGCCCGGGC TGTCGGCTGG ACTATGAGGA GTGCAACATG 
CAGAGCTGTC AGGAGGTGCG CAAACTCAGT GCCTGGACAC CGTGGCTAAC GGTGACCGGG 
AGCGGAAATT CAAGTGAACC GCACATGGAG AGGCGATTCA GGTTCGTCTG TCGTGCCACC 
AGTCCAGATC CGAGTTCCGT CCGAATTGGC CTGCCCAAGG AGGAGTCGCG CAATTGTCAT 
GCTGATGGCA GCTGCCAGCG GCAGGTTGAT CACGACTCCG CGGACTCCGA TGCTGCGGAC 
TGGTCACCAT GCAGCGTCAG TTGCGGTGGT GGTGTCCAGC AACGTCACCG CGGTCGTGGA 
AGCCAGAGTC GGGTATGCAA TATACACGCC TGCCCGGCGG AAGAACAGCT AAGTAGTAAT 
AGCCTGGATA ACGAGTTGGA ACACGGCGAA TGGGGCTGCT GGTCCGAGTG GTCTGCCTGC 
TCGGTGACCT GCGGATTGGG ACTACGGCGG AGGACACGTC GTTGCCTAGC GGGTCACGAC 
CGGCTATGTC AGGGTCGTGC ACTCGAAGAA CAGAAGTGTG AAATGGTGCC ATGCGAAGAT 
TTCCTAGGTT GGAGCGCTTG GAGCGAGTGG TCGAGCTGCT CCAGCGATGG CATCCGACTG 
AGACATCGCC GCTGCCTGGT GGAGCAGCCC GGTTCGATGG AGTGCCGTGG TGCGGAGTTC 
GAGAAGACCG CCTGTGTGCC CAATGAGTGC GAGGAAACCC AAACTGCTTC GACTGCCACG 
CTACCCATCG TGATCTTCGT TGGCCTGCTT TTCACAGTGG CCTGTTGCCT GGCCACCTAC 
AGATTCACCA AGAAACGATT CATGTTAAGT GCTGAGGAGG CATTAAACAA AACCACGACG 
ACAACAGCCA GTTTCGACAC CTATCCCAAT CAGTACTCCA GTCTGCCCAC CAAGGATTAC 
TACGATCAGC GACCCAAACG GCAGTCCAGC TTCCGGATGC CGGCCAAGAC CAGTAATTTG 
GGCAACGGCA ATGGCACCCT CAATCGGAAC AACATGCACC AAAATAATAC GCCCAAAGTT 
CTGGCCAAAA CGTATATCGA CTGCGAATCC GGAACGATCA AACGACAATC GGCACTGAAC 
AATTGCCGCA GTAATATCGA CGACGAGAAG TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163430.1
CDS	272..3547
Translation	MLILKLPKMFSQLWLLLILSLLTLEGPQPSTGSGYTTKSSVDLLENDSRYISYQDLMSTAKRFYDPETTWYSEMLFDVARNQVIVGARDTLYRMSFDLEPLERASWGATPSEIAMCQAKGQSERWCRNYVRVLHSYGENQLYACGTNAFQPSCSWRQMENLTVTGVDSGVVKCPFHPQANSTSLLQSNGQLFVGTATDFSGSDVAILRTGVESNKRFLRTKQYNNNWLSGAQFVGSFEAGHFVYFLLRESAAEHMSCGKVIYSRVARVCKNDVGGGGQLLRDNWTSFLKARLNCSLPGEYPYYFDEIQGMTYAESESILYATFRTSGSSIFGSAVCAYNLSSINAAFDGPFKQQEHSDAAWKTVNTNQRSQFQCGTSSIGHWLESSRYQLMDEAVQPIGAEPLYHSKLEQFGRLALDIINTKTEQVHVLFVASSGNHIKKLSVKYDGDGVQTCLVELWQADDTGTSSLLNMAYLKVSDSLYLGTDLALTRIPAQHCSRHVSQSSCLNSMDPYCGWNELVERCMPQPQDSSVLQHWHQAPQITCPVLNAPIDGGWSTWSPWAVCQQHEQPDSNCQCRQRSCNNPQPQHGGATCEGISTQVTNCTQHGGWTEWSAWSPCSQTCGIAVKIRRRTCGNPRPAFGGRTCVGSEQSEMYCRHLPPCPVAKPQSVDGGWGPWGEWSECSAQCGGGFRMRRRECNDPAPLNGGMECPGCRLDYEECNMQSCQEVRKLSAWTPWLTVTGSGNSSEPHMERRFRFVCRATSPDPSSVRIGLPKEESRNCHADGSCQRQVDHDSADSDAADWSPCSVSCGGGVQQRHRGRGSQSRVCNIHACPAEEQLSSNSLDNELEHGEWGCWSEWSACSVTCGLGLRRRTRRCLAGHDRLCQGRALEEQKCEMVPCEDFLGWSAWSEWSSCSSDGIRLRHRRCLVEQPGSMECRGAEFEKTACVPNECEETQTASTATLPIVIFVGLLFTVACCLATYRFTKKRFMLSAEEALNKTTTTTASFDTYPNQYSSLPTKDYYDQRPKRQSSFRMPAKTSNLGNGNGTLNRNNMHQNNTPKVLAKTYIDCESGTIKRQSALNNCRSNIDDEKF

Target ORF information:

RefSeq Version	NM_001169959.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster semaphorin 5c, transcript variant B (Sema5c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169959.2

ORF Insert Sequence:

ATGTTAATAC TGAAACTTCC CAAAATGTTT AGCCAACTGT GGCTTCTTCT GATTCTATCG 
CTCTTAACCC TAGAAGGCCC ACAGCCTTCG ACGGGCAGTG GTTATACGAC AAAATCAAGT 
GTGGATTTGC TGGAAAACGA TTCGCGATAC ATCTCATATC AAGATTTAAT GTCGACGGCC 
AAACGATTTT ACGATCCCGA AACGACCTGG TATTCGGAAA TGCTATTCGA CGTGGCCAGG 
AACCAAGTGA TTGTCGGCGC CAGGGACACC CTGTACCGCA TGTCTTTCGA CCTGGAGCCG 
CTGGAGCGGG CCAGCTGGGG GGCCACGCCA TCGGAGATCG CCATGTGCCA GGCAAAAGGC 
CAGTCGGAGC GATGGTGTCG CAACTATGTG CGCGTGCTCC ACAGCTACGG TGAGAACCAG 
CTGTACGCGT GTGGGACGAA CGCCTTCCAG CCGAGCTGTT CGTGGCGCCA GATGGAGAAC 
CTCACAGTCA CCGGCGTGGA CAGCGGCGTG GTCAAGTGTC CATTTCATCC GCAGGCGAAC 
AGCACCAGTC TGCTGCAGTC CAATGGGCAA CTCTTTGTGG GCACCGCCAC TGATTTCTCG 
GGCAGCGATG TGGCCATCCT GCGAACAGGA GTGGAGTCCA ATAAGCGTTT CCTCCGCACC 
AAGCAATATA ATAACAACTG GCTGAGTGGA GCCCAATTCG TGGGAAGCTT CGAGGCTGGC 
CACTTTGTAT ATTTTCTGCT ACGCGAATCG GCCGCCGAGC ACATGAGTTG TGGCAAGGTG 
ATATACTCCA GAGTTGCTAG GGTGTGCAAG AATGATGTGG GCGGTGGTGG TCAGTTGCTA 
AGGGACAATT GGACATCGTT TCTGAAGGCT CGTCTCAATT GCTCACTGCC TGGCGAATAT 
CCTTATTATT TCGATGAGAT CCAAGGCATG ACGTATGCCG AATCCGAATC CATTCTCTAT 
GCCACCTTCA GAACGTCGGG ATCCAGCATA TTTGGATCAG CTGTTTGTGC CTACAACCTG 
AGCTCCATTA ACGCAGCCTT CGATGGACCC TTTAAGCAGC AGGAGCACTC GGATGCGGCC 
TGGAAAACGG TCAACACGAA TCAGCGGAGT CAGTTCCAGT GCGGAACGAG TAGTATTGGC 
CACTGGCTGG AGAGCTCACG TTACCAACTG ATGGATGAGG CAGTGCAGCC AATTGGAGCA 
GAGCCATTGT ACCACTCCAA ACTGGAACAA TTCGGCCGCC TGGCGTTGGA CATCATAAAC 
ACAAAGACGG AGCAAGTGCA CGTGCTCTTC GTGGCCAGCA GTGGGAATCA CATAAAGAAA 
TTGTCCGTAA AGTACGATGG CGATGGAGTT CAGACATGTC TGGTGGAACT TTGGCAGGCG 
GACGATACTG GTACTAGTAG CCTACTGAAT ATGGCCTATC TGAAAGTCAG TGATTCCCTC 
TATCTGGGCA CCGATCTGGC GCTCACCCGG ATTCCCGCCC AGCACTGCAG TCGCCATGTG 
TCGCAGTCAA GTTGTCTGAA CTCCATGGAT CCCTACTGCG GCTGGAACGA ACTGGTGGAG 
CGCTGCATGC CACAGCCACA GGATTCCTCT GTACTGCAAC ACTGGCATCA GGCGCCACAG 
ATCACCTGTC CTGTACTAAA TGCCCCCATC GATGGCGGTT GGTCTACCTG GAGTCCATGG 
GCCGTTTGCC AGCAGCACGA GCAGCCGGAT AGCAATTGCC AGTGCCGACA GCGCAGTTGC 
AACAATCCTC AGCCACAACA TGGAGGTGCC ACCTGCGAGG GCATCAGCAC CCAAGTGACG 
AACTGCACGC AGCACGGAGG ATGGACGGAG TGGTCAGCAT GGTCGCCCTG CTCCCAGACC 
TGCGGCATTG CCGTGAAGAT CCGACGACGC ACCTGTGGAA ATCCGCGTCC GGCATTCGGC 
GGTCGCACCT GTGTGGGATC GGAGCAGTCG GAGATGTACT GCCGCCACTT GCCACCGTGC 
CCGGTGGCCA AGCCGCAGTC CGTGGATGGC GGATGGGGAC CGTGGGGCGA GTGGAGTGAG 
TGCAGTGCCC AGTGCGGCGG TGGTTTCCGA ATGCGACGAC GTGAGTGCAA CGATCCGGCT 
CCACTCAATG GCGGAATGGA GTGCCCGGGC TGTCGGCTGG ACTATGAGGA GTGCAACATG 
CAGAGCTGTC AGGAGGTGCG CAAACTCAGT GCCTGGACAC CGTGGCTAAC GGTGACCGGG 
AGCGGAAATT CAAGTGAACC GCACATGGAG AGGCGATTCA GGTTCGTCTG TCGTGCCACC 
AGTCCAGATC CGAGTTCCGT CCGAATTGGC CTGCCCAAGG AGGAGTCGCG CAATTGTCAT 
GCTGATGGCA GCTGCCAGCG GCAGGTTGAT CACGACTCCG CGGACTCCGA TGCTGCGGAC 
TGGTCACCAT GCAGCGTCAG TTGCGGTGGT GGTGTCCAGC AACGTCACCG CGGTCGTGGA 
AGCCAGAGTC GGGTATGCAA TATACACGCC TGCCCGGCGG AAGAACAGCT AAGTAGTAAT 
AGCCTGGATA ACGAGTTGGA ACACGGCGAA TGGGGCTGCT GGTCCGAGTG GTCTGCCTGC 
TCGGTGACCT GCGGATTGGG ACTACGGCGG AGGACACGTC GTTGCCTAGC GGGTCACGAC 
CGGCTATGTC AGGGTCGTGC ACTCGAAGAA CAGAAGTGTG AAATGGTGCC ATGCGAAGAT 
TTCCTAGGTT GGAGCGCTTG GAGCGAGTGG TCGAGCTGCT CCAGCGATGG CATCCGACTG 
AGACATCGCC GCTGCCTGGT GGAGCAGCCC GGTTCGATGG AGTGCCGTGG TGCGGAGTTC 
GAGAAGACCG CCTGTGTGCC CAATGAGTGC GAGGAAACCC AAACTGCTTC GACTGCCACG 
CTACCCATCG TGATCTTCGT TGGCCTGCTT TTCACAGTGG CCTGTTGCCT GGCCACCTAC 
AGATTCACCA AGAAACGATT CATGTTAAGT GCTGAGGAGG CATTAAACAA AACCACGACG 
ACAACAGCCA GTTTCGACAC CTATCCCAAT CAGTACTCCA GTCTGCCCAC CAAGGATTAC 
TACGATCAGC GACCCAAACG GCAGTCCAGC TTCCGGATGC CGGCCAAGAC CAGTAATTTG 
GGCAACGGCA ATGGCACCCT CAATCGGAAC AACATGCACC AAAATAATAC GCCCAAAGTT 
CTGGCCAAAA CGTATATCGA CTGCGAATCC GGAACGATCA AACGACAATC GGCACTGAAC 
AATTGCCGCA GTAATATCGA CGACGAGAAG TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sema5c cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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