aft cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	aft
Entrez Gene ID	37034
Full Name	adrift
Synonyms	CG5032,Dmel\CG5032,EG:52C10.7
General protein information	Preferred Names adrift Names CG5032 gene product from transcript CG5032-RA CG5032-PA aft-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54E9-54E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_058065.3	NP_477413.1	adrift

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005415	XP_315423.4
Drosophila melanogaster (fruit fly)	aft	NP_477413.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following aft gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aft cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10394	NM_058065.3 Latest version!	Drosophila melanogaster adrift (aft), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aft ( NM_058065.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477413.1 Drosophila melanogaster adrift (aft), mRNA.

Price & Availability↑

CloneID	OFa10394
Clone ID Related Accession (Same CDS sequence)	NM_058065.3
Accession Version	NM_058065.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	adrift
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_058065.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTTTC GTTCGTCTCC GCAGGGAAAG CCACACCCAA TGACGGACTA TCAGTCCATC 
CGGCCTTCCG AAGTGGAGCA GCTCTTCGAG AAGAAGTTCC ACTATCAGAA GCCGAAAGGA 
AACAAATCCT GGCAGTTGCC GCCACCGGAT CAGGCTCTCT TCAGTGAGTT CTACCAGTTC 
GAGGCGCTGC AGGGACTGCG CGAGCAGCTG AATGCGGTTA AAAGCAAACT CAATGACTAT 
GGCGTGCAGG AATGGAGTGC TCACACCAAC AGGCGTGACC CTTCTGGCGA GGTGTCCTGG 
CGCCTGAAAA ACGACACGAA GGCCGAATTT GTAACTGTGG CATGGTGCAA GTTGTTCGAG 
TGCCTGCATC GTTATCCATT GGTCACGAAG CCGGCTGTCA ACAGTATGCA TCTCTGCGAG 
GCACCCGGTG CCTTCATAGC ATCTCTGAAT CATTACCTTC ATAGCAAATA TGAAAAGGAT 
GAGATTAAAT GGCGCTGGCG CTCAACTACT TTGAATCCGT ACTATGAAGG CAATGCTATA 
AACCAGATGA TCAGCGATGA CAGGTTCATC GTCCACACGC TGGACAACTG GTTCTTTCAC 
AAAGATCTAA CTGGAAATCT ACTAGATGTG GCCAACATTG ACCACCTGGT GGAACGTTGT 
GAGGTGGAGT TCCAGGGACA GGTGGATCTT GTCACTGCCG ATGGTTCCAT CGATTGTGCC 
GCACAGCCTG ATTGCCAGGA AGAGATAGTG GTTCGCCTTT TCTTTGCTGA AGTACTCAGT 
GCCTTAAGAA TTCTCTCAAG TGGCGGTAAC TTCCTAGTGA AGATGTTTAC CCTCTTCGAG 
GCTTGCAGTG TATCTCTACT TTATACACTC AATTGCATCT TTGAGGAGGT ACACATCTTT 
AAGCCGGCTA CTTCCAAGCG GGGAAATTCG GAAGTGTATG TTATCTGCCT GAACTACAAC 
AAGGACCATC CCGACTTGCC TCGTTTGCTA GAGGAGATAA AGAGCAAGCT TGCTCAGCCG 
AATGACACAT TGGTAATGCC ACTGTTTGCC AAGTTCCAGA TTCCCCACGA CTTTCTCATG 
CAGCACGAGA TCGCCTGCCG TATGTACATG AAGCTTCAAA CGGATGCCAT CGAGGGCAGT 
ATCTATGCCT ACGAGTCCAA TGATCGTCAT TATCTGAGAC ATCTTCATCA CCTGCGCAGT 
CTTGTCGCGA ACACCTACTA CAGTCTATAC AAAGTGAAGC CATTAGAAGA TAGCCTGTGC 
ATCGTGGACA AGGAGGCCAC CAGTAAGGCT CTGGGATTCC AAGTGCCTGT CTATGGCGGT 
TCCTACACAG AAAGGGAGTC CTTGAAACAC GGTGACCTTC TCAAGCAGAT TTACTGCTTG 
CGTCGGGAAT TTAATCAACT GGAGAAGTGT CTTAACAATA GAACACCGTA TTCATACGTT 
AAGAACAGAA CGGCACCCTT GAATCTTCAC ATTTCACGAG GAGCACCTGT TCAGAGCCTG 
CAAAGCAGCA TGTTTGCCTC GGAACCAATA CTCATTCTGC GCCTTCGCAT CCTGGACACA 
TTTGAACTCG ACCCTGTCTG GCAGTCTGCG CCAAAGTGCC AGTTGGAAAG CAAGACACTA 
TGCTACCTGC CACCAACAGA AGACGAGGCA TTCCACACCG CCCAGCAGCG CTTTTTCATT 
GATCTCCTGG AAGAAGTAAA GAAATTGAAG CCCGACAGCA TTGTCTTCCA CAAGTTCCTG 
TTTCTCACCC ATTACGCGGC GTCGCTGCTC TTATTTCTCA TAGAGAGCGT TTATCAGGAT 
TGTTGTTTTA ATAGCAATCA AGCGCAAACT CTCACGTTGA GTAAGCTAAA AGATACGGCT 
AACAGTGCCT TGGAACAGGT GCTGGAACTT CTTAAGGACG AGCAGGCGGG GGCCATTCAC 
AGCCTGCTGG ACATTAAGGA GCTGCAGAAG AACCAGTTCA GCAAGGCCCT CATTCAGCAC 
AACAACAGCA TTGTAATGAC CTGCTTCCGT AGCATGTTGG GCGAGGAATC ATTTCCCATG 
CCCGTAGCTC CGACATCCAA CTCAGATGTC GGTTCCATCC AAGAGTCGGC AGCTGTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477413.1
CDS	69..2171
Translation	MSFRSSPQGKPHPMTDYQSIRPSEVEQLFEKKFHYQKPKGNKSWQLPPPDQALFSEFYQFEALQGLREQLNAVKSKLNDYGVQEWSAHTNRRDPSGEVSWRLKNDTKAEFVTVAWCKLFECLHRYPLVTKPAVNSMHLCEAPGAFIASLNHYLHSKYEKDEIKWRWRSTTLNPYYEGNAINQMISDDRFIVHTLDNWFFHKDLTGNLLDVANIDHLVERCEVEFQGQVDLVTADGSIDCAAQPDCQEEIVVRLFFAEVLSALRILSSGGNFLVKMFTLFEACSVSLLYTLNCIFEEVHIFKPATSKRGNSEVYVICLNYNKDHPDLPRLLEEIKSKLAQPNDTLVMPLFAKFQIPHDFLMQHEIACRMYMKLQTDAIEGSIYAYESNDRHYLRHLHHLRSLVANTYYSLYKVKPLEDSLCIVDKEATSKALGFQVPVYGGSYTERESLKHGDLLKQIYCLRREFNQLEKCLNNRTPYSYVKNRTAPLNLHISRGAPVQSLQSSMFASEPILILRLRILDTFELDPVWQSAPKCQLESKTLCYLPPTEDEAFHTAQQRFFIDLLEEVKKLKPDSIVFHKFLFLTHYAASLLLFLIESVYQDCCFNSNQAQTLTLSKLKDTANSALEQVLELLKDEQAGAIHSLLDIKELQKNQFSKALIQHNNSIVMTCFRSMLGEESFPMPVAPTSNSDVGSIQESAAVF

Target ORF information:

RefSeq Version	NM_058065.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adrift (aft), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058065.3

ORF Insert Sequence:

ATGAGCTTTC GTTCGTCTCC GCAGGGAAAG CCACACCCAA TGACGGACTA TCAGTCCATC 
CGGCCTTCCG AAGTGGAGCA GCTCTTCGAG AAGAAGTTCC ACTATCAGAA GCCGAAAGGA 
AACAAATCCT GGCAGTTGCC GCCACCGGAT CAGGCTCTCT TCAGTGAGTT CTACCAGTTC 
GAGGCGCTGC AGGGACTGCG CGAGCAGCTG AATGCGGTTA AAAGCAAACT CAATGACTAT 
GGCGTGCAGG AATGGAGTGC TCACACCAAC AGGCGTGACC CTTCTGGCGA GGTGTCCTGG 
CGCCTGAAAA ACGACACGAA GGCCGAATTT GTAACTGTGG CATGGTGCAA GTTGTTCGAG 
TGCCTGCATC GTTATCCATT GGTCACGAAG CCGGCTGTCA ACAGTATGCA TCTCTGCGAG 
GCACCCGGTG CCTTCATAGC ATCTCTGAAT CATTACCTTC ATAGCAAATA TGAAAAGGAT 
GAGATTAAAT GGCGCTGGCG CTCAACTACT TTGAATCCGT ACTATGAAGG CAATGCTATA 
AACCAGATGA TCAGCGATGA CAGGTTCATC GTCCACACGC TGGACAACTG GTTCTTTCAC 
AAAGATCTAA CTGGAAATCT ACTAGATGTG GCCAACATTG ACCACCTGGT GGAACGTTGT 
GAGGTGGAGT TCCAGGGACA GGTGGATCTT GTCACTGCCG ATGGTTCCAT CGATTGTGCC 
GCACAGCCTG ATTGCCAGGA AGAGATAGTG GTTCGCCTTT TCTTTGCTGA AGTACTCAGT 
GCCTTAAGAA TTCTCTCAAG TGGCGGTAAC TTCCTAGTGA AGATGTTTAC CCTCTTCGAG 
GCTTGCAGTG TATCTCTACT TTATACACTC AATTGCATCT TTGAGGAGGT ACACATCTTT 
AAGCCGGCTA CTTCCAAGCG GGGAAATTCG GAAGTGTATG TTATCTGCCT GAACTACAAC 
AAGGACCATC CCGACTTGCC TCGTTTGCTA GAGGAGATAA AGAGCAAGCT TGCTCAGCCG 
AATGACACAT TGGTAATGCC ACTGTTTGCC AAGTTCCAGA TTCCCCACGA CTTTCTCATG 
CAGCACGAGA TCGCCTGCCG TATGTACATG AAGCTTCAAA CGGATGCCAT CGAGGGCAGT 
ATCTATGCCT ACGAGTCCAA TGATCGTCAT TATCTGAGAC ATCTTCATCA CCTGCGCAGT 
CTTGTCGCGA ACACCTACTA CAGTCTATAC AAAGTGAAGC CATTAGAAGA TAGCCTGTGC 
ATCGTGGACA AGGAGGCCAC CAGTAAGGCT CTGGGATTCC AAGTGCCTGT CTATGGCGGT 
TCCTACACAG AAAGGGAGTC CTTGAAACAC GGTGACCTTC TCAAGCAGAT TTACTGCTTG 
CGTCGGGAAT TTAATCAACT GGAGAAGTGT CTTAACAATA GAACACCGTA TTCATACGTT 
AAGAACAGAA CGGCACCCTT GAATCTTCAC ATTTCACGAG GAGCACCTGT TCAGAGCCTG 
CAAAGCAGCA TGTTTGCCTC GGAACCAATA CTCATTCTGC GCCTTCGCAT CCTGGACACA 
TTTGAACTCG ACCCTGTCTG GCAGTCTGCG CCAAAGTGCC AGTTGGAAAG CAAGACACTA 
TGCTACCTGC CACCAACAGA AGACGAGGCA TTCCACACCG CCCAGCAGCG CTTTTTCATT 
GATCTCCTGG AAGAAGTAAA GAAATTGAAG CCCGACAGCA TTGTCTTCCA CAAGTTCCTG 
TTTCTCACCC ATTACGCGGC GTCGCTGCTC TTATTTCTCA TAGAGAGCGT TTATCAGGAT 
TGTTGTTTTA ATAGCAATCA AGCGCAAACT CTCACGTTGA GTAAGCTAAA AGATACGGCT 
AACAGTGCCT TGGAACAGGT GCTGGAACTT CTTAAGGACG AGCAGGCGGG GGCCATTCAC 
AGCCTGCTGG ACATTAAGGA GCTGCAGAAG AACCAGTTCA GCAAGGCCCT CATTCAGCAC 
AACAACAGCA TTGTAATGAC CTGCTTCCGT AGCATGTTGG GCGAGGAATC ATTTCCCATG 
CCCGTAGCTC CGACATCCAA CTCAGATGTC GGTTCCATCC AAGAGTCGGC AGCTGTATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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aft cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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