Smurf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smurf
Entrez Gene ID	36999
Full Name	SMAD specific E3 ubiquitin protein ligase
Synonyms	CG4943,D-smurf,DSmurf,Dmel\CG4943,Dsmurf,LACK,Lack,SMURF,Smurf ubiquitin ligase,d-smurf,dSmurf,dSmurf1,lack,smurf
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase Names CG4943 gene product from transcript CG4943-RB CG4943-PA CG4943-PB Smurf-PA Smurf-PB Smurf1 lethal with a checkpoint kinase smurf E3 ligase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54C12-54D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079055.3	NP_523779.1	SMAD specific E3 ubiquitin protein ligase, isoform A
NM_001274132.1	NP_001261061.1	SMAD specific E3 ubiquitin protein ligase, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	lack	NP_523779.1
	SMURF2	XP_001109913.2
Bos taurus (cattle)	SMURF2	XP_002696169.3
Gallus gallus (chicken)	SMURF2	XP_425380.2
Homo sapiens (human)	SMURF2	NP_073576.1
Danio rerio (zebrafish)	smurf2	NP_001107898.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011242	XP_309403.3
Pan troglodytes (chimpanzee)	SMURF2	XP_511577.3
Canis lupus familiaris (dog)	SMURF2	XP_537589.4
Mus musculus (house mouse)	Smurf2	NP_079757.2
Rattus norvegicus (Norway rat)	Smurf2	NP_001100531.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together, our results demonstrate that transcriptional repression of Ed followed by Smurf-mediated downregulation of pretranslated Ed in amnioserosa regulates the establishment of a taut leading edge during dorsal closure
Title: Smurf Downregulates Echinoid in the Amnioserosa To Regulate

Interaction with Slimb leads to the ubiquitination and degradation of dSmurf, resulting in negative regulation of dSmurf-mediated Yki phosphorylation and activity in the Hippo pathway. Thus our study revealed a new regulatory mechanism of the Hippo pathway which may provide implications for developing tumor treatment.
Title: The Drosophila F-box protein Slimb controls dSmurf protein turnover to regulate the Hippo pathway.

Sufficient knock-down of dSmurf increases the protein abundance of Wts and thus increases phosphorylation level at S168 of Yki, the key downstream target of Wts in the Hippo pathway.
Title: Ubiquitin E3 ligase dSmurf is essential for Wts protein turnover and Hippo signaling.

Smurf-mediated differential proteolysis generates dynamic BMP signaling in germline stem cells during Drosophila testis development.
Title: Smurf-mediated differential proteolysis generates dynamic BMP signaling in germline stem cells during Drosophila testis development.

Smo interacts with Smurf and promotes it to mediate Patched ubiquitination by targeting the K1261 site in Ptc.
Title: Activation of Smurf E3 ligase promoted by smoothened regulates hedgehog signaling through targeting patched turnover.

The following Smurf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smurf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10361	NM_001274132.1 Latest version!	Drosophila melanogaster SMAD specific E3 ubiquitin protein ligase (Smurf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa10361	NM_079055.3 Latest version!	Drosophila melanogaster SMAD specific E3 ubiquitin protein ligase (Smurf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smurf ( NM_079055.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523779.1 Drosophila melanogaster SMAD specific E3 ubiquitin protein ligase (Smurf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10361
Clone ID Related Accession (Same CDS sequence)	NM_079055.3 , NM_001274132.1
Accession Version	NM_079055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	SMAD specific E3 ubiquitin protein ligase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_079055.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAAAT TGGATTACCC ACGTCGCAAT GGTACACACA AAGTTCGAAT AACGATATTG 
TGTGCTAGAA ACTTGGCCCG CAAGGATTTA TTTCGTCTAC CCGATCCCTT TGCTAAGGTG 
CAGGTGGATG GAACTGGGCA GGTGTACTCG ACGGAAATAA GCAAATCCTC TCTGGATCCA 
AAATGGAATG CACATTACGA TTTGTTTTTG GGCATTGGGG ATGCCATTAC GATAACTGTG 
TGGAATCAGC GAAAGATACA CAAGGGCAGT GGATTCCTGG GCTGTGTGAG GATACCGGCG 
TTCAACATAC AGAGCCTCAA AGGAGCTGGG TTTCAACGCT TGGATCTGGG AAAACTGTCG 
CCGGATGACG ATGAGCTTGT ACGTGGTCAA ATCATCATTT CGTTGCTCTC GAAGGACGGT 
CCTAGTAGTG GTAATCCTCT GGCCATTGTA GGACCCAGTG GTGATGTCCG TGGACCGTCG 
GAAGATGACT CCTCGGAAGA TAGTTTGCCA GAGGGTTGGG AGGAGCGGCG TACGGACAAC 
GGTCGAGTTT ACTATGTGAA CCATGCCACC AAATCGACGC AGTGGGATCG ACCAAGACAG 
CCAGGAGTAG TTGGATCCAG TCACGCCACT TCACCACAGC AACGGCACAA CACGCACAAT 
GGCAACAGCG GTGATCGACA AGCTCCGGCT GGGCCAACCC GCTCTACAAC TTGCACGAAC 
CTGATGAATA ACGGTCATCG CAGCCGGGAC TTGTCTGTGA CGGCTTCAGA TGAACGTAGA 
CACTCTACGG AAATCCTGTC CAGCGTGGGC AAGGAGAACA CCAGCCCCAC AACGCCCGTT 
TCGGCCACGA CAACGCCTGG CAAAAAAACA TCCTCATCGA ACTCATCCTC GGCAGGTGGT 
CGCACTTTGG AGCAGCGTCC GACAAACGAA CCAGCAACAC CCACCAGCAG TACGACATCG 
GCGTCGGTTC GCCTGCATAG CAACGACAAT CATGTAAAAA CACCAAAGCA CCAAACGAAT 
GGCCATGCTC CGCCAGAGTC CACGCCCACA TCCCCCACGG GTCAGCAGAA CTATGTAAAT 
GGAAATGCTC AGAATGGAAG TACCAGCGGA AATGGAAGTG GCCAGGCGGC GCAGCCCCAG 
AGTGCTAGTA ACGGATGGAC GCAAGAAGAT GCCGCAACGA CTACTTCACC TTCAACCACA 
ACCTCTCCTC CCAGGCATAG TCAAAGTCCG CCAACTCCAA ATATATCGCC GCCAGCTTCA 
GTGACACCCT CTGCAAATGG AAATGTGCAC AGTCCGAATG CGAACAGCAC ACCAGCCGGA 
AGCGGAGGAG GAAGTAGATC CTATACAGCA GCAACGCCCG GTCAACGCTC TCAGCGTCGG 
AGCTCGCGGC AACAAGGTGA GGAGTCCTCC ACCAGACGAC GGTCTTCACG GGGAACAAGA 
AATGGGGGCA CATCTGGCGG CGGAGGTGGA GGTGGATCTG GGCAGAGGTA CGCCTCGGCA 
GCCATTGCAG CTGCCAATCA GGCAGCACGA CCATTTTTGG ATCTGCCGCC CGGCTATGAA 
ATGCGAACAA CGCAACAAGG GCAGGTATAC TTTTATCACA TACCCACTGG TGTATCCACG 
TGGCATGATC CTCGGATACC CAGAGATTTC GACACCCAAC ATCTAACGCT TGACGCTATC 
GGACCATTGC CGAGTGGTTG GGAACAGCGA AAGACGGCCT CCGGAAGGGT TTACTTTGTG 
GACCACAACA ACCGAACCAC ACAGTTTACG GATCCCCGGC TAAGTGGCAG TATTTTGCAA 
ATGATTCGAC GTGGAACCGT ACCACCAACA TCCGCTGCAA ATGCTGGAAC ACCAGCTCCG 
CCATCGGCTA CTCCTGCTAC CCCCTCGGCC GCAGCAGCAG TTCCACCACA GGCGACGCCG 
GCGAGCAACG CAACTCCAAC GACGCTTACA ACTACAACAA ACCCGCCCCA TCGTATTGTT 
CCGGATCTGC CACAGGGATT GCTTGAAGGC GCCGACCTTT TGCCCAAGTA CCGCCGCGAT 
TTGGTTGGCA AGCTGCGGGC TCTGCGCACG GAGCTGCAAA CGATGCAGCC GCAATCCGGC 
CACTGCCGTT TGGAGGTGTC ACGCAACGAG ATTTTCGAGG AGAGCTATAG ACTGATAATG 
AAAATGCGGG CCAAGGACAT GCGAAAGCGT CTAATGGTTA AATTTAAGGG CGAGGAGGGC 
CTGGACTATG GTGGCGTAGC ACGCGAGTGG TTGCATCTGC TTTCCCGAGA GATGTTGAAT 
CCGCAGTATG GCCTATTCCA GTACAGTAGG GATGATCACT ACACGCTGCA AATTAATCCA 
GACTCCGGTG TGAATCCCGA TCACTTGTCC TATTTTCATT TCGTGGGTCG CACACTAGGG 
ATTGCCGTGT TCCACGGTCA CTGTCTGGAT GGTGGGTTCA CTACACCCTT CTACAAGCAG 
CTGCTGAACA AGCCGATTAC GCTTGGTGAT ATTGAAGGCG TGGATCCGGA CCTACATCGC 
AGCCTCACCT GGATGCTGGA AAGCAACATC AGCGGCATCA TTGAATCCAC ATTCAGTGTA 
GAGAACAATA GCTTTGGTGC TCTGGTGGTG CATGAACTGA AGCCTGGCGG TGCCTCCATT 
CCAGTGACGG AGGAGAACAA ACGCGAGTAT GTCAAGCTCT ATGTGAACTA TCGTTTTATG 
CGCGGCATTG AACAACAATT TTTGGCTCTG CAGAAAGGTT TTTGTGAGCT TATACCCAGC 
CATCTCCTAA GACCGTTTGA TGAGCGCGAA TTGGAATTGG TCATTGGTGG CATCTCTAGC 
ATAGATGTCA ACGACTGGCG GAACAATACG AGACTGAAGC ACTGTACGAA CGAAACGACG 
CAGGTGTTGT GGTTCTGGCA GGTGGTCGAG TCCTATAGTT CTGAGATGAG AGCCCGTCTG 
CTGCAATTCG TGACAGGATC ATCGCGAGTG CCGCTGCAGG GCTTCAGAGC GCTACAGGGC 
TCCACGGGAG CGGTGGGACC ACGGCTGTTT ACTATTCACC TGACTGCCGA CGTGCCCACG 
CAGAACCTGC CCAAGGCGCA CACCTGCTTT AACCGGATCG ATTTGCCGCC CTACGAGACG 
TACCAGCTAC TTTGCGACAA GCTGACGCAG GCCGTTGAGG AGACCTGTGG ATTTGCCGTG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523779.1
CDS	905..4090
Translation	MNKLDYPRRNGTHKVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAITITVWNQRKIHKGSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDELVRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSSHATSPQQRHNTHNGNSGDRQAPAGPTRSTTCTNLMNNGHRSRDLSVTASDERRHSTEILSSVGKENTSPTTPVSATTTPGKKTSSSNSSSAGGRTLEQRPTNEPATPTSSTTSASVRLHSNDNHVKTPKHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDAATTTSPSTTTSPPRHSQSPPTPNISPPASVTPSANGNVHSPNANSTPAGSGGGSRSYTAATPGQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSGGGGGGGSGQRYASAAIAAANQAARPFLDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRLSGSILQMIRRGTVPPTSAANAGTPAPPSATPATPSAAAAVPPQATPASNATPTTLTTTTNPPHRIVPDLPQGLLEGADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_079055.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster SMAD specific E3 ubiquitin protein ligase (Smurf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079055.3

ORF Insert Sequence:

ATGAATAAAT TGGATTACCC ACGTCGCAAT GGTACACACA AAGTTCGAAT AACGATATTG 
TGTGCTAGAA ACTTGGCCCG CAAGGATTTA TTTCGTCTAC CCGATCCCTT TGCTAAGGTG 
CAGGTGGATG GAACTGGGCA GGTGTACTCG ACGGAAATAA GCAAATCCTC TCTGGATCCA 
AAATGGAATG CACATTACGA TTTGTTTTTG GGCATTGGGG ATGCCATTAC GATAACTGTG 
TGGAATCAGC GAAAGATACA CAAGGGCAGT GGATTCCTGG GCTGTGTGAG GATACCGGCG 
TTCAACATAC AGAGCCTCAA AGGAGCTGGG TTTCAACGCT TGGATCTGGG AAAACTGTCG 
CCGGATGACG ATGAGCTTGT ACGTGGTCAA ATCATCATTT CGTTGCTCTC GAAGGACGGT 
CCTAGTAGTG GTAATCCTCT GGCCATTGTA GGACCCAGTG GTGATGTCCG TGGACCGTCG 
GAAGATGACT CCTCGGAAGA TAGTTTGCCA GAGGGTTGGG AGGAGCGGCG TACGGACAAC 
GGTCGAGTTT ACTATGTGAA CCATGCCACC AAATCGACGC AGTGGGATCG ACCAAGACAG 
CCAGGAGTAG TTGGATCCAG TCACGCCACT TCACCACAGC AACGGCACAA CACGCACAAT 
GGCAACAGCG GTGATCGACA AGCTCCGGCT GGGCCAACCC GCTCTACAAC TTGCACGAAC 
CTGATGAATA ACGGTCATCG CAGCCGGGAC TTGTCTGTGA CGGCTTCAGA TGAACGTAGA 
CACTCTACGG AAATCCTGTC CAGCGTGGGC AAGGAGAACA CCAGCCCCAC AACGCCCGTT 
TCGGCCACGA CAACGCCTGG CAAAAAAACA TCCTCATCGA ACTCATCCTC GGCAGGTGGT 
CGCACTTTGG AGCAGCGTCC GACAAACGAA CCAGCAACAC CCACCAGCAG TACGACATCG 
GCGTCGGTTC GCCTGCATAG CAACGACAAT CATGTAAAAA CACCAAAGCA CCAAACGAAT 
GGCCATGCTC CGCCAGAGTC CACGCCCACA TCCCCCACGG GTCAGCAGAA CTATGTAAAT 
GGAAATGCTC AGAATGGAAG TACCAGCGGA AATGGAAGTG GCCAGGCGGC GCAGCCCCAG 
AGTGCTAGTA ACGGATGGAC GCAAGAAGAT GCCGCAACGA CTACTTCACC TTCAACCACA 
ACCTCTCCTC CCAGGCATAG TCAAAGTCCG CCAACTCCAA ATATATCGCC GCCAGCTTCA 
GTGACACCCT CTGCAAATGG AAATGTGCAC AGTCCGAATG CGAACAGCAC ACCAGCCGGA 
AGCGGAGGAG GAAGTAGATC CTATACAGCA GCAACGCCCG GTCAACGCTC TCAGCGTCGG 
AGCTCGCGGC AACAAGGTGA GGAGTCCTCC ACCAGACGAC GGTCTTCACG GGGAACAAGA 
AATGGGGGCA CATCTGGCGG CGGAGGTGGA GGTGGATCTG GGCAGAGGTA CGCCTCGGCA 
GCCATTGCAG CTGCCAATCA GGCAGCACGA CCATTTTTGG ATCTGCCGCC CGGCTATGAA 
ATGCGAACAA CGCAACAAGG GCAGGTATAC TTTTATCACA TACCCACTGG TGTATCCACG 
TGGCATGATC CTCGGATACC CAGAGATTTC GACACCCAAC ATCTAACGCT TGACGCTATC 
GGACCATTGC CGAGTGGTTG GGAACAGCGA AAGACGGCCT CCGGAAGGGT TTACTTTGTG 
GACCACAACA ACCGAACCAC ACAGTTTACG GATCCCCGGC TAAGTGGCAG TATTTTGCAA 
ATGATTCGAC GTGGAACCGT ACCACCAACA TCCGCTGCAA ATGCTGGAAC ACCAGCTCCG 
CCATCGGCTA CTCCTGCTAC CCCCTCGGCC GCAGCAGCAG TTCCACCACA GGCGACGCCG 
GCGAGCAACG CAACTCCAAC GACGCTTACA ACTACAACAA ACCCGCCCCA TCGTATTGTT 
CCGGATCTGC CACAGGGATT GCTTGAAGGC GCCGACCTTT TGCCCAAGTA CCGCCGCGAT 
TTGGTTGGCA AGCTGCGGGC TCTGCGCACG GAGCTGCAAA CGATGCAGCC GCAATCCGGC 
CACTGCCGTT TGGAGGTGTC ACGCAACGAG ATTTTCGAGG AGAGCTATAG ACTGATAATG 
AAAATGCGGG CCAAGGACAT GCGAAAGCGT CTAATGGTTA AATTTAAGGG CGAGGAGGGC 
CTGGACTATG GTGGCGTAGC ACGCGAGTGG TTGCATCTGC TTTCCCGAGA GATGTTGAAT 
CCGCAGTATG GCCTATTCCA GTACAGTAGG GATGATCACT ACACGCTGCA AATTAATCCA 
GACTCCGGTG TGAATCCCGA TCACTTGTCC TATTTTCATT TCGTGGGTCG CACACTAGGG 
ATTGCCGTGT TCCACGGTCA CTGTCTGGAT GGTGGGTTCA CTACACCCTT CTACAAGCAG 
CTGCTGAACA AGCCGATTAC GCTTGGTGAT ATTGAAGGCG TGGATCCGGA CCTACATCGC 
AGCCTCACCT GGATGCTGGA AAGCAACATC AGCGGCATCA TTGAATCCAC ATTCAGTGTA 
GAGAACAATA GCTTTGGTGC TCTGGTGGTG CATGAACTGA AGCCTGGCGG TGCCTCCATT 
CCAGTGACGG AGGAGAACAA ACGCGAGTAT GTCAAGCTCT ATGTGAACTA TCGTTTTATG 
CGCGGCATTG AACAACAATT TTTGGCTCTG CAGAAAGGTT TTTGTGAGCT TATACCCAGC 
CATCTCCTAA GACCGTTTGA TGAGCGCGAA TTGGAATTGG TCATTGGTGG CATCTCTAGC 
ATAGATGTCA ACGACTGGCG GAACAATACG AGACTGAAGC ACTGTACGAA CGAAACGACG 
CAGGTGTTGT GGTTCTGGCA GGTGGTCGAG TCCTATAGTT CTGAGATGAG AGCCCGTCTG 
CTGCAATTCG TGACAGGATC ATCGCGAGTG CCGCTGCAGG GCTTCAGAGC GCTACAGGGC 
TCCACGGGAG CGGTGGGACC ACGGCTGTTT ACTATTCACC TGACTGCCGA CGTGCCCACG 
CAGAACCTGC CCAAGGCGCA CACCTGCTTT AACCGGATCG ATTTGCCGCC CTACGAGACG 
TACCAGCTAC TTTGCGACAA GCTGACGCAG GCCGTTGAGG AGACCTGTGG ATTTGCCGTG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf ( NM_001274132.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261061.1 Drosophila melanogaster SMAD specific E3 ubiquitin protein ligase (Smurf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10361
Clone ID Related Accession (Same CDS sequence)	NM_079055.3 , NM_001274132.1
Accession Version	NM_001274132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	SMAD specific E3 ubiquitin protein ligase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAAAT TGGATTACCC ACGTCGCAAT GGTACACACA AAGTTCGAAT AACGATATTG 
TGTGCTAGAA ACTTGGCCCG CAAGGATTTA TTTCGTCTAC CCGATCCCTT TGCTAAGGTG 
CAGGTGGATG GAACTGGGCA GGTGTACTCG ACGGAAATAA GCAAATCCTC TCTGGATCCA 
AAATGGAATG CACATTACGA TTTGTTTTTG GGCATTGGGG ATGCCATTAC GATAACTGTG 
TGGAATCAGC GAAAGATACA CAAGGGCAGT GGATTCCTGG GCTGTGTGAG GATACCGGCG 
TTCAACATAC AGAGCCTCAA AGGAGCTGGG TTTCAACGCT TGGATCTGGG AAAACTGTCG 
CCGGATGACG ATGAGCTTGT ACGTGGTCAA ATCATCATTT CGTTGCTCTC GAAGGACGGT 
CCTAGTAGTG GTAATCCTCT GGCCATTGTA GGACCCAGTG GTGATGTCCG TGGACCGTCG 
GAAGATGACT CCTCGGAAGA TAGTTTGCCA GAGGGTTGGG AGGAGCGGCG TACGGACAAC 
GGTCGAGTTT ACTATGTGAA CCATGCCACC AAATCGACGC AGTGGGATCG ACCAAGACAG 
CCAGGAGTAG TTGGATCCAG TCACGCCACT TCACCACAGC AACGGCACAA CACGCACAAT 
GGCAACAGCG GTGATCGACA AGCTCCGGCT GGGCCAACCC GCTCTACAAC TTGCACGAAC 
CTGATGAATA ACGGTCATCG CAGCCGGGAC TTGTCTGTGA CGGCTTCAGA TGAACGTAGA 
CACTCTACGG AAATCCTGTC CAGCGTGGGC AAGGAGAACA CCAGCCCCAC AACGCCCGTT 
TCGGCCACGA CAACGCCTGG CAAAAAAACA TCCTCATCGA ACTCATCCTC GGCAGGTGGT 
CGCACTTTGG AGCAGCGTCC GACAAACGAA CCAGCAACAC CCACCAGCAG TACGACATCG 
GCGTCGGTTC GCCTGCATAG CAACGACAAT CATGTAAAAA CACCAAAGCA CCAAACGAAT 
GGCCATGCTC CGCCAGAGTC CACGCCCACA TCCCCCACGG GTCAGCAGAA CTATGTAAAT 
GGAAATGCTC AGAATGGAAG TACCAGCGGA AATGGAAGTG GCCAGGCGGC GCAGCCCCAG 
AGTGCTAGTA ACGGATGGAC GCAAGAAGAT GCCGCAACGA CTACTTCACC TTCAACCACA 
ACCTCTCCTC CCAGGCATAG TCAAAGTCCG CCAACTCCAA ATATATCGCC GCCAGCTTCA 
GTGACACCCT CTGCAAATGG AAATGTGCAC AGTCCGAATG CGAACAGCAC ACCAGCCGGA 
AGCGGAGGAG GAAGTAGATC CTATACAGCA GCAACGCCCG GTCAACGCTC TCAGCGTCGG 
AGCTCGCGGC AACAAGGTGA GGAGTCCTCC ACCAGACGAC GGTCTTCACG GGGAACAAGA 
AATGGGGGCA CATCTGGCGG CGGAGGTGGA GGTGGATCTG GGCAGAGGTA CGCCTCGGCA 
GCCATTGCAG CTGCCAATCA GGCAGCACGA CCATTTTTGG ATCTGCCGCC CGGCTATGAA 
ATGCGAACAA CGCAACAAGG GCAGGTATAC TTTTATCACA TACCCACTGG TGTATCCACG 
TGGCATGATC CTCGGATACC CAGAGATTTC GACACCCAAC ATCTAACGCT TGACGCTATC 
GGACCATTGC CGAGTGGTTG GGAACAGCGA AAGACGGCCT CCGGAAGGGT TTACTTTGTG 
GACCACAACA ACCGAACCAC ACAGTTTACG GATCCCCGGC TAAGTGGCAG TATTTTGCAA 
ATGATTCGAC GTGGAACCGT ACCACCAACA TCCGCTGCAA ATGCTGGAAC ACCAGCTCCG 
CCATCGGCTA CTCCTGCTAC CCCCTCGGCC GCAGCAGCAG TTCCACCACA GGCGACGCCG 
GCGAGCAACG CAACTCCAAC GACGCTTACA ACTACAACAA ACCCGCCCCA TCGTATTGTT 
CCGGATCTGC CACAGGGATT GCTTGAAGGC GCCGACCTTT TGCCCAAGTA CCGCCGCGAT 
TTGGTTGGCA AGCTGCGGGC TCTGCGCACG GAGCTGCAAA CGATGCAGCC GCAATCCGGC 
CACTGCCGTT TGGAGGTGTC ACGCAACGAG ATTTTCGAGG AGAGCTATAG ACTGATAATG 
AAAATGCGGG CCAAGGACAT GCGAAAGCGT CTAATGGTTA AATTTAAGGG CGAGGAGGGC 
CTGGACTATG GTGGCGTAGC ACGCGAGTGG TTGCATCTGC TTTCCCGAGA GATGTTGAAT 
CCGCAGTATG GCCTATTCCA GTACAGTAGG GATGATCACT ACACGCTGCA AATTAATCCA 
GACTCCGGTG TGAATCCCGA TCACTTGTCC TATTTTCATT TCGTGGGTCG CACACTAGGG 
ATTGCCGTGT TCCACGGTCA CTGTCTGGAT GGTGGGTTCA CTACACCCTT CTACAAGCAG 
CTGCTGAACA AGCCGATTAC GCTTGGTGAT ATTGAAGGCG TGGATCCGGA CCTACATCGC 
AGCCTCACCT GGATGCTGGA AAGCAACATC AGCGGCATCA TTGAATCCAC ATTCAGTGTA 
GAGAACAATA GCTTTGGTGC TCTGGTGGTG CATGAACTGA AGCCTGGCGG TGCCTCCATT 
CCAGTGACGG AGGAGAACAA ACGCGAGTAT GTCAAGCTCT ATGTGAACTA TCGTTTTATG 
CGCGGCATTG AACAACAATT TTTGGCTCTG CAGAAAGGTT TTTGTGAGCT TATACCCAGC 
CATCTCCTAA GACCGTTTGA TGAGCGCGAA TTGGAATTGG TCATTGGTGG CATCTCTAGC 
ATAGATGTCA ACGACTGGCG GAACAATACG AGACTGAAGC ACTGTACGAA CGAAACGACG 
CAGGTGTTGT GGTTCTGGCA GGTGGTCGAG TCCTATAGTT CTGAGATGAG AGCCCGTCTG 
CTGCAATTCG TGACAGGATC ATCGCGAGTG CCGCTGCAGG GCTTCAGAGC GCTACAGGGC 
TCCACGGGAG CGGTGGGACC ACGGCTGTTT ACTATTCACC TGACTGCCGA CGTGCCCACG 
CAGAACCTGC CCAAGGCGCA CACCTGCTTT AACCGGATCG ATTTGCCGCC CTACGAGACG 
TACCAGCTAC TTTGCGACAA GCTGACGCAG GCCGTTGAGG AGACCTGTGG ATTTGCCGTG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261061.1
CDS	905..4090
Translation	MNKLDYPRRNGTHKVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAITITVWNQRKIHKGSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDELVRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSSHATSPQQRHNTHNGNSGDRQAPAGPTRSTTCTNLMNNGHRSRDLSVTASDERRHSTEILSSVGKENTSPTTPVSATTTPGKKTSSSNSSSAGGRTLEQRPTNEPATPTSSTTSASVRLHSNDNHVKTPKHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDAATTTSPSTTTSPPRHSQSPPTPNISPPASVTPSANGNVHSPNANSTPAGSGGGSRSYTAATPGQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSGGGGGGGSGQRYASAAIAAANQAARPFLDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRLSGSILQMIRRGTVPPTSAANAGTPAPPSATPATPSAAAAVPPQATPASNATPTTLTTTTNPPHRIVPDLPQGLLEGADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_001274132.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster SMAD specific E3 ubiquitin protein ligase (Smurf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274132.1

ORF Insert Sequence:

ATGAATAAAT TGGATTACCC ACGTCGCAAT GGTACACACA AAGTTCGAAT AACGATATTG 
TGTGCTAGAA ACTTGGCCCG CAAGGATTTA TTTCGTCTAC CCGATCCCTT TGCTAAGGTG 
CAGGTGGATG GAACTGGGCA GGTGTACTCG ACGGAAATAA GCAAATCCTC TCTGGATCCA 
AAATGGAATG CACATTACGA TTTGTTTTTG GGCATTGGGG ATGCCATTAC GATAACTGTG 
TGGAATCAGC GAAAGATACA CAAGGGCAGT GGATTCCTGG GCTGTGTGAG GATACCGGCG 
TTCAACATAC AGAGCCTCAA AGGAGCTGGG TTTCAACGCT TGGATCTGGG AAAACTGTCG 
CCGGATGACG ATGAGCTTGT ACGTGGTCAA ATCATCATTT CGTTGCTCTC GAAGGACGGT 
CCTAGTAGTG GTAATCCTCT GGCCATTGTA GGACCCAGTG GTGATGTCCG TGGACCGTCG 
GAAGATGACT CCTCGGAAGA TAGTTTGCCA GAGGGTTGGG AGGAGCGGCG TACGGACAAC 
GGTCGAGTTT ACTATGTGAA CCATGCCACC AAATCGACGC AGTGGGATCG ACCAAGACAG 
CCAGGAGTAG TTGGATCCAG TCACGCCACT TCACCACAGC AACGGCACAA CACGCACAAT 
GGCAACAGCG GTGATCGACA AGCTCCGGCT GGGCCAACCC GCTCTACAAC TTGCACGAAC 
CTGATGAATA ACGGTCATCG CAGCCGGGAC TTGTCTGTGA CGGCTTCAGA TGAACGTAGA 
CACTCTACGG AAATCCTGTC CAGCGTGGGC AAGGAGAACA CCAGCCCCAC AACGCCCGTT 
TCGGCCACGA CAACGCCTGG CAAAAAAACA TCCTCATCGA ACTCATCCTC GGCAGGTGGT 
CGCACTTTGG AGCAGCGTCC GACAAACGAA CCAGCAACAC CCACCAGCAG TACGACATCG 
GCGTCGGTTC GCCTGCATAG CAACGACAAT CATGTAAAAA CACCAAAGCA CCAAACGAAT 
GGCCATGCTC CGCCAGAGTC CACGCCCACA TCCCCCACGG GTCAGCAGAA CTATGTAAAT 
GGAAATGCTC AGAATGGAAG TACCAGCGGA AATGGAAGTG GCCAGGCGGC GCAGCCCCAG 
AGTGCTAGTA ACGGATGGAC GCAAGAAGAT GCCGCAACGA CTACTTCACC TTCAACCACA 
ACCTCTCCTC CCAGGCATAG TCAAAGTCCG CCAACTCCAA ATATATCGCC GCCAGCTTCA 
GTGACACCCT CTGCAAATGG AAATGTGCAC AGTCCGAATG CGAACAGCAC ACCAGCCGGA 
AGCGGAGGAG GAAGTAGATC CTATACAGCA GCAACGCCCG GTCAACGCTC TCAGCGTCGG 
AGCTCGCGGC AACAAGGTGA GGAGTCCTCC ACCAGACGAC GGTCTTCACG GGGAACAAGA 
AATGGGGGCA CATCTGGCGG CGGAGGTGGA GGTGGATCTG GGCAGAGGTA CGCCTCGGCA 
GCCATTGCAG CTGCCAATCA GGCAGCACGA CCATTTTTGG ATCTGCCGCC CGGCTATGAA 
ATGCGAACAA CGCAACAAGG GCAGGTATAC TTTTATCACA TACCCACTGG TGTATCCACG 
TGGCATGATC CTCGGATACC CAGAGATTTC GACACCCAAC ATCTAACGCT TGACGCTATC 
GGACCATTGC CGAGTGGTTG GGAACAGCGA AAGACGGCCT CCGGAAGGGT TTACTTTGTG 
GACCACAACA ACCGAACCAC ACAGTTTACG GATCCCCGGC TAAGTGGCAG TATTTTGCAA 
ATGATTCGAC GTGGAACCGT ACCACCAACA TCCGCTGCAA ATGCTGGAAC ACCAGCTCCG 
CCATCGGCTA CTCCTGCTAC CCCCTCGGCC GCAGCAGCAG TTCCACCACA GGCGACGCCG 
GCGAGCAACG CAACTCCAAC GACGCTTACA ACTACAACAA ACCCGCCCCA TCGTATTGTT 
CCGGATCTGC CACAGGGATT GCTTGAAGGC GCCGACCTTT TGCCCAAGTA CCGCCGCGAT 
TTGGTTGGCA AGCTGCGGGC TCTGCGCACG GAGCTGCAAA CGATGCAGCC GCAATCCGGC 
CACTGCCGTT TGGAGGTGTC ACGCAACGAG ATTTTCGAGG AGAGCTATAG ACTGATAATG 
AAAATGCGGG CCAAGGACAT GCGAAAGCGT CTAATGGTTA AATTTAAGGG CGAGGAGGGC 
CTGGACTATG GTGGCGTAGC ACGCGAGTGG TTGCATCTGC TTTCCCGAGA GATGTTGAAT 
CCGCAGTATG GCCTATTCCA GTACAGTAGG GATGATCACT ACACGCTGCA AATTAATCCA 
GACTCCGGTG TGAATCCCGA TCACTTGTCC TATTTTCATT TCGTGGGTCG CACACTAGGG 
ATTGCCGTGT TCCACGGTCA CTGTCTGGAT GGTGGGTTCA CTACACCCTT CTACAAGCAG 
CTGCTGAACA AGCCGATTAC GCTTGGTGAT ATTGAAGGCG TGGATCCGGA CCTACATCGC 
AGCCTCACCT GGATGCTGGA AAGCAACATC AGCGGCATCA TTGAATCCAC ATTCAGTGTA 
GAGAACAATA GCTTTGGTGC TCTGGTGGTG CATGAACTGA AGCCTGGCGG TGCCTCCATT 
CCAGTGACGG AGGAGAACAA ACGCGAGTAT GTCAAGCTCT ATGTGAACTA TCGTTTTATG 
CGCGGCATTG AACAACAATT TTTGGCTCTG CAGAAAGGTT TTTGTGAGCT TATACCCAGC 
CATCTCCTAA GACCGTTTGA TGAGCGCGAA TTGGAATTGG TCATTGGTGG CATCTCTAGC 
ATAGATGTCA ACGACTGGCG GAACAATACG AGACTGAAGC ACTGTACGAA CGAAACGACG 
CAGGTGTTGT GGTTCTGGCA GGTGGTCGAG TCCTATAGTT CTGAGATGAG AGCCCGTCTG 
CTGCAATTCG TGACAGGATC ATCGCGAGTG CCGCTGCAGG GCTTCAGAGC GCTACAGGGC 
TCCACGGGAG CGGTGGGACC ACGGCTGTTT ACTATTCACC TGACTGCCGA CGTGCCCACG 
CAGAACCTGC CCAAGGCGCA CACCTGCTTT AACCGGATCG ATTTGCCGCC CTACGAGACG 
TACCAGCTAC TTTGCGACAA GCTGACGCAG GCCGTTGAGG AGACCTGTGG ATTTGCCGTG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Smurf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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