CG4853 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG4853
Entrez Gene ID	36974
Full Name	CG4853 gene product from transcript CG4853-RB
Synonyms	Dmel\CG4853
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54C1-54C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_166238.4	NP_725689.2	uncharacterized protein, isoform A
NM_137378.4	NP_611222.2	uncharacterized protein, isoform B
NM_001299606.1	NP_001286535.1	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG4853 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG4853 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13049	NM_166238.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG4853), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13049	NM_137378.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG4853), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa21842	NM_001299606.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG4853), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG4853 ( NM_166238.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725689.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG4853), mRNA.

Price & Availability↑

CloneID	OFa13049
Clone ID Related Accession (Same CDS sequence)	NM_166238.4 , NM_137378.4
Accession Version	NM_166238.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166238.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGAAG ATATCCTAAA CGCTGTGGAG TCCCTGTTCG GCAAGGATGT CAAGATCGTG 
GACTGCGAAA CCTCATCCTC GCTGCAGCAG GAGGAGGTGT TGCTGGTCAA CGGAGTGCCG 
GTGAAGCTGG ATGGGCTGCC GGCGGATGAT GCCAGTGCCA TAAAGGCAGC TTTGCTGGAG 
GGGCAGATGC CATCGGTTGA GCACCTGAAT CAATTGCTCT TCCGCGCCGG ATTGGCCCAA 
CAGCCCATCG AGGTAGAGAC CTCGTTGACC GTCAAGTCCT CTCTGACCAC CACGGAGGAG 
GTGCTGGTCT CGCGCAACGG ACAAATCCTA GACGAACGCA CCGTGGAGAC CAAGGAAAAC 
TTTAACCATC AGTCGCACCA GAAAGAGATC TGGCATCCTG TCAAGCAGCA GTCGGCCTTG 
GCGCGTGCCA CTCCCGAGAA TGCCCTCAAA TACCGAACCA CTTCGAATTC CACGTTCGCA 
TCGCAGGCCA CAGACTCGGA CGAGCGTCCA GTTGATCCTT TGGGCCGGCA GCTGAACCAA 
ACCTTCTCGA ATGCTTCCCT TATGTCCAGC AGCTCTGCTA TTACTGGATC CGATCAGGTC 
ATCGGATCTG TATCATCCAC CACTATTGGG GACAAAAGCT CGAATATAAG CAGCTGCGAC 
TCCGGGCACG ATGGCCTCTT CCACAGCGTG TCGATGAGTG CACCCTGGTC ACATCCACGT 
GACACCTTAA CCGACTCTTT GTACTGCGAC ATTCCCAGCT CCGCAGATGA GTCGGATGCG 
GTTTCTATCC TAAACACCCA CTTGAAGATC ACAGAGCCAT TCGATTGCCA AACAGCGCCA 
GTTTACGCCA AAGACATACC CAAGAAGGGC AACCTGGATT CGGTGGTCAA CCTGCTTATC 
AATAATAATA CTAAGGATTT GCGGTTTGCC TTTCTGACCA CTTCCCGCTT GTTTTCACCA 
CCACACCAGC TGCTTTCCAA AATCATTGCC AAGATTGATA ATGAGGAGGA AAACGTGTTA 
CGACTTTTGA ACGAATGGCT AGACACCTGT CCCGGGGACT TTAGCCATGA ACTGCTATTG 
CAGGAGCTGG AAAATAAGCG TAATGTAAAA GGATTGGCAG CTTTTTTGGA TGGAATTCCC 
CAAGCTTTGG CGCAAAAGGA CGAAAATGGA CGAAGTCAAC TAAATAACCT GCTTACCAAG 
CAATCGTCGG CCAGCTCGTT GGGACGTCAG AGCAGTATTA AGTCGCTGAA ACGCTTTGCC 
GCCAATGTCT ACAAAACAGA CAATGTGCTA AACAACTGCA GCAGTGCCTT CGAGTTGGCT 
CACCAACTTT ATGCCATCGA GTACGCCTAT CTGTCACAAA TACGTCTTGA GGAGTTTGTG 
GAAATCCTCG AAAAGGATGA GCTGAAGACC TGCATAAGCC AAACCAAGGC GGGCACCTTG 
GGTTCGAATT GCATCAGCCA GGTGACAATC GACAGTTACG TGCAGTGGTT CAACCAGCTA 
AGCTACTTGA CTGCAACGGA AATATTAAAG TTGGGCAAAA AGTCCCAGAG AGCACAAATG 
ATCGACTTCT GGGTGGAAAC AGCTCTGGAA TGCTTTAACA CAGGCAACTT CAACAGCTTG 
ATGGCCATTC TAACAGCATT GAATCTTACT GCCATAGCTC GCCTAAAGAA AACGTGGGCC 
AAAGTGCAGA CGACAAAGTT CGAGGGTTTG GAGCATCAAA TGGATCCCAG TTCGAACTTT 
CTTAACTACA GATCCACCAT GAAGGCAGCT GTTTGGCGTT CGGAAAGGGA AATGGCCAAC 
GAAATTGAGC GTGCCATCAT CCCATTCTTC TCGCTGTTCC TCAAAGATTT GCATGCCATT 
AACGAGAGCC ATGAAACAAA ATTGGCCAAC GGATACATAA ACTTTGAGAA GTGCCTTCAT 
TTGGGCACCC AGCTGCGGAA TTTTGGCAAA TGGCAGCGAT TGGACTGTCC ATACGAGCAG 
TTGCCTAGTG TGGTGTCCTA CCTGTTGAAG GCGGAGGTGC TGAGCGAGGA TCTTTTGATG 
AAGGCCTCCT ACGAAAGCGA GCCACCCGAG ACCGGCGAGG AGAAGGATCA CTACAAGACG 
TTGAAAGCTG CAAGGAAATC GAACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725689.2
CDS	359..2488
Translation	MDEDILNAVESLFGKDVKIVDCETSSSLQQEEVLLVNGVPVKLDGLPADDASAIKAALLEGQMPSVEHLNQLLFRAGLAQQPIEVETSLTVKSSLTTTEEVLVSRNGQILDERTVETKENFNHQSHQKEIWHPVKQQSALARATPENALKYRTTSNSTFASQATDSDERPVDPLGRQLNQTFSNASLMSSSSAITGSDQVIGSVSSTTIGDKSSNISSCDSGHDGLFHSVSMSAPWSHPRDTLTDSLYCDIPSSADESDAVSILNTHLKITEPFDCQTAPVYAKDIPKKGNLDSVVNLLINNNTKDLRFAFLTTSRLFSPPHQLLSKIIAKIDNEEENVLRLLNEWLDTCPGDFSHELLLQELENKRNVKGLAAFLDGIPQALAQKDENGRSQLNNLLTKQSSASSLGRQSSIKSLKRFAANVYKTDNVLNNCSSAFELAHQLYAIEYAYLSQIRLEEFVEILEKDELKTCISQTKAGTLGSNCISQVTIDSYVQWFNQLSYLTATEILKLGKKSQRAQMIDFWVETALECFNTGNFNSLMAILTALNLTAIARLKKTWAKVQTTKFEGLEHQMDPSSNFLNYRSTMKAAVWRSEREMANEIERAIIPFFSLFLKDLHAINESHETKLANGYINFEKCLHLGTQLRNFGKWQRLDCPYEQLPSVVSYLLKAEVLSEDLLMKASYESEPPETGEEKDHYKTLKAARKSNT

Target ORF information:

RefSeq Version	NM_166238.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG4853), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166238.4

ORF Insert Sequence:

ATGGACGAAG ATATCCTAAA CGCTGTGGAG TCCCTGTTCG GCAAGGATGT CAAGATCGTG 
GACTGCGAAA CCTCATCCTC GCTGCAGCAG GAGGAGGTGT TGCTGGTCAA CGGAGTGCCG 
GTGAAGCTGG ATGGGCTGCC GGCGGATGAT GCCAGTGCCA TAAAGGCAGC TTTGCTGGAG 
GGGCAGATGC CATCGGTTGA GCACCTGAAT CAATTGCTCT TCCGCGCCGG ATTGGCCCAA 
CAGCCCATCG AGGTAGAGAC CTCGTTGACC GTCAAGTCCT CTCTGACCAC CACGGAGGAG 
GTGCTGGTCT CGCGCAACGG ACAAATCCTA GACGAACGCA CCGTGGAGAC CAAGGAAAAC 
TTTAACCATC AGTCGCACCA GAAAGAGATC TGGCATCCTG TCAAGCAGCA GTCGGCCTTG 
GCGCGTGCCA CTCCCGAGAA TGCCCTCAAA TACCGAACCA CTTCGAATTC CACGTTCGCA 
TCGCAGGCCA CAGACTCGGA CGAGCGTCCA GTTGATCCTT TGGGCCGGCA GCTGAACCAA 
ACCTTCTCGA ATGCTTCCCT TATGTCCAGC AGCTCTGCTA TTACTGGATC CGATCAGGTC 
ATCGGATCTG TATCATCCAC CACTATTGGG GACAAAAGCT CGAATATAAG CAGCTGCGAC 
TCCGGGCACG ATGGCCTCTT CCACAGCGTG TCGATGAGTG CACCCTGGTC ACATCCACGT 
GACACCTTAA CCGACTCTTT GTACTGCGAC ATTCCCAGCT CCGCAGATGA GTCGGATGCG 
GTTTCTATCC TAAACACCCA CTTGAAGATC ACAGAGCCAT TCGATTGCCA AACAGCGCCA 
GTTTACGCCA AAGACATACC CAAGAAGGGC AACCTGGATT CGGTGGTCAA CCTGCTTATC 
AATAATAATA CTAAGGATTT GCGGTTTGCC TTTCTGACCA CTTCCCGCTT GTTTTCACCA 
CCACACCAGC TGCTTTCCAA AATCATTGCC AAGATTGATA ATGAGGAGGA AAACGTGTTA 
CGACTTTTGA ACGAATGGCT AGACACCTGT CCCGGGGACT TTAGCCATGA ACTGCTATTG 
CAGGAGCTGG AAAATAAGCG TAATGTAAAA GGATTGGCAG CTTTTTTGGA TGGAATTCCC 
CAAGCTTTGG CGCAAAAGGA CGAAAATGGA CGAAGTCAAC TAAATAACCT GCTTACCAAG 
CAATCGTCGG CCAGCTCGTT GGGACGTCAG AGCAGTATTA AGTCGCTGAA ACGCTTTGCC 
GCCAATGTCT ACAAAACAGA CAATGTGCTA AACAACTGCA GCAGTGCCTT CGAGTTGGCT 
CACCAACTTT ATGCCATCGA GTACGCCTAT CTGTCACAAA TACGTCTTGA GGAGTTTGTG 
GAAATCCTCG AAAAGGATGA GCTGAAGACC TGCATAAGCC AAACCAAGGC GGGCACCTTG 
GGTTCGAATT GCATCAGCCA GGTGACAATC GACAGTTACG TGCAGTGGTT CAACCAGCTA 
AGCTACTTGA CTGCAACGGA AATATTAAAG TTGGGCAAAA AGTCCCAGAG AGCACAAATG 
ATCGACTTCT GGGTGGAAAC AGCTCTGGAA TGCTTTAACA CAGGCAACTT CAACAGCTTG 
ATGGCCATTC TAACAGCATT GAATCTTACT GCCATAGCTC GCCTAAAGAA AACGTGGGCC 
AAAGTGCAGA CGACAAAGTT CGAGGGTTTG GAGCATCAAA TGGATCCCAG TTCGAACTTT 
CTTAACTACA GATCCACCAT GAAGGCAGCT GTTTGGCGTT CGGAAAGGGA AATGGCCAAC 
GAAATTGAGC GTGCCATCAT CCCATTCTTC TCGCTGTTCC TCAAAGATTT GCATGCCATT 
AACGAGAGCC ATGAAACAAA ATTGGCCAAC GGATACATAA ACTTTGAGAA GTGCCTTCAT 
TTGGGCACCC AGCTGCGGAA TTTTGGCAAA TGGCAGCGAT TGGACTGTCC ATACGAGCAG 
TTGCCTAGTG TGGTGTCCTA CCTGTTGAAG GCGGAGGTGC TGAGCGAGGA TCTTTTGATG 
AAGGCCTCCT ACGAAAGCGA GCCACCCGAG ACCGGCGAGG AGAAGGATCA CTACAAGACG 
TTGAAAGCTG CAAGGAAATC GAACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG4853 ( NM_137378.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611222.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG4853), mRNA.

Price & Availability↑

CloneID	OFa13049
Clone ID Related Accession (Same CDS sequence)	NM_166238.4 , NM_137378.4
Accession Version	NM_137378.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137378.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGAAG ATATCCTAAA CGCTGTGGAG TCCCTGTTCG GCAAGGATGT CAAGATCGTG 
GACTGCGAAA CCTCATCCTC GCTGCAGCAG GAGGAGGTGT TGCTGGTCAA CGGAGTGCCG 
GTGAAGCTGG ATGGGCTGCC GGCGGATGAT GCCAGTGCCA TAAAGGCAGC TTTGCTGGAG 
GGGCAGATGC CATCGGTTGA GCACCTGAAT CAATTGCTCT TCCGCGCCGG ATTGGCCCAA 
CAGCCCATCG AGGTAGAGAC CTCGTTGACC GTCAAGTCCT CTCTGACCAC CACGGAGGAG 
GTGCTGGTCT CGCGCAACGG ACAAATCCTA GACGAACGCA CCGTGGAGAC CAAGGAAAAC 
TTTAACCATC AGTCGCACCA GAAAGAGATC TGGCATCCTG TCAAGCAGCA GTCGGCCTTG 
GCGCGTGCCA CTCCCGAGAA TGCCCTCAAA TACCGAACCA CTTCGAATTC CACGTTCGCA 
TCGCAGGCCA CAGACTCGGA CGAGCGTCCA GTTGATCCTT TGGGCCGGCA GCTGAACCAA 
ACCTTCTCGA ATGCTTCCCT TATGTCCAGC AGCTCTGCTA TTACTGGATC CGATCAGGTC 
ATCGGATCTG TATCATCCAC CACTATTGGG GACAAAAGCT CGAATATAAG CAGCTGCGAC 
TCCGGGCACG ATGGCCTCTT CCACAGCGTG TCGATGAGTG CACCCTGGTC ACATCCACGT 
GACACCTTAA CCGACTCTTT GTACTGCGAC ATTCCCAGCT CCGCAGATGA GTCGGATGCG 
GTTTCTATCC TAAACACCCA CTTGAAGATC ACAGAGCCAT TCGATTGCCA AACAGCGCCA 
GTTTACGCCA AAGACATACC CAAGAAGGGC AACCTGGATT CGGTGGTCAA CCTGCTTATC 
AATAATAATA CTAAGGATTT GCGGTTTGCC TTTCTGACCA CTTCCCGCTT GTTTTCACCA 
CCACACCAGC TGCTTTCCAA AATCATTGCC AAGATTGATA ATGAGGAGGA AAACGTGTTA 
CGACTTTTGA ACGAATGGCT AGACACCTGT CCCGGGGACT TTAGCCATGA ACTGCTATTG 
CAGGAGCTGG AAAATAAGCG TAATGTAAAA GGATTGGCAG CTTTTTTGGA TGGAATTCCC 
CAAGCTTTGG CGCAAAAGGA CGAAAATGGA CGAAGTCAAC TAAATAACCT GCTTACCAAG 
CAATCGTCGG CCAGCTCGTT GGGACGTCAG AGCAGTATTA AGTCGCTGAA ACGCTTTGCC 
GCCAATGTCT ACAAAACAGA CAATGTGCTA AACAACTGCA GCAGTGCCTT CGAGTTGGCT 
CACCAACTTT ATGCCATCGA GTACGCCTAT CTGTCACAAA TACGTCTTGA GGAGTTTGTG 
GAAATCCTCG AAAAGGATGA GCTGAAGACC TGCATAAGCC AAACCAAGGC GGGCACCTTG 
GGTTCGAATT GCATCAGCCA GGTGACAATC GACAGTTACG TGCAGTGGTT CAACCAGCTA 
AGCTACTTGA CTGCAACGGA AATATTAAAG TTGGGCAAAA AGTCCCAGAG AGCACAAATG 
ATCGACTTCT GGGTGGAAAC AGCTCTGGAA TGCTTTAACA CAGGCAACTT CAACAGCTTG 
ATGGCCATTC TAACAGCATT GAATCTTACT GCCATAGCTC GCCTAAAGAA AACGTGGGCC 
AAAGTGCAGA CGACAAAGTT CGAGGGTTTG GAGCATCAAA TGGATCCCAG TTCGAACTTT 
CTTAACTACA GATCCACCAT GAAGGCAGCT GTTTGGCGTT CGGAAAGGGA AATGGCCAAC 
GAAATTGAGC GTGCCATCAT CCCATTCTTC TCGCTGTTCC TCAAAGATTT GCATGCCATT 
AACGAGAGCC ATGAAACAAA ATTGGCCAAC GGATACATAA ACTTTGAGAA GTGCCTTCAT 
TTGGGCACCC AGCTGCGGAA TTTTGGCAAA TGGCAGCGAT TGGACTGTCC ATACGAGCAG 
TTGCCTAGTG TGGTGTCCTA CCTGTTGAAG GCGGAGGTGC TGAGCGAGGA TCTTTTGATG 
AAGGCCTCCT ACGAAAGCGA GCCACCCGAG ACCGGCGAGG AGAAGGATCA CTACAAGACG 
TTGAAAGCTG CAAGGAAATC GAACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611222.2
CDS	522..2651
Translation	MDEDILNAVESLFGKDVKIVDCETSSSLQQEEVLLVNGVPVKLDGLPADDASAIKAALLEGQMPSVEHLNQLLFRAGLAQQPIEVETSLTVKSSLTTTEEVLVSRNGQILDERTVETKENFNHQSHQKEIWHPVKQQSALARATPENALKYRTTSNSTFASQATDSDERPVDPLGRQLNQTFSNASLMSSSSAITGSDQVIGSVSSTTIGDKSSNISSCDSGHDGLFHSVSMSAPWSHPRDTLTDSLYCDIPSSADESDAVSILNTHLKITEPFDCQTAPVYAKDIPKKGNLDSVVNLLINNNTKDLRFAFLTTSRLFSPPHQLLSKIIAKIDNEEENVLRLLNEWLDTCPGDFSHELLLQELENKRNVKGLAAFLDGIPQALAQKDENGRSQLNNLLTKQSSASSLGRQSSIKSLKRFAANVYKTDNVLNNCSSAFELAHQLYAIEYAYLSQIRLEEFVEILEKDELKTCISQTKAGTLGSNCISQVTIDSYVQWFNQLSYLTATEILKLGKKSQRAQMIDFWVETALECFNTGNFNSLMAILTALNLTAIARLKKTWAKVQTTKFEGLEHQMDPSSNFLNYRSTMKAAVWRSEREMANEIERAIIPFFSLFLKDLHAINESHETKLANGYINFEKCLHLGTQLRNFGKWQRLDCPYEQLPSVVSYLLKAEVLSEDLLMKASYESEPPETGEEKDHYKTLKAARKSNT

Target ORF information:

RefSeq Version	NM_137378.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG4853), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137378.4

ORF Insert Sequence:

ATGGACGAAG ATATCCTAAA CGCTGTGGAG TCCCTGTTCG GCAAGGATGT CAAGATCGTG 
GACTGCGAAA CCTCATCCTC GCTGCAGCAG GAGGAGGTGT TGCTGGTCAA CGGAGTGCCG 
GTGAAGCTGG ATGGGCTGCC GGCGGATGAT GCCAGTGCCA TAAAGGCAGC TTTGCTGGAG 
GGGCAGATGC CATCGGTTGA GCACCTGAAT CAATTGCTCT TCCGCGCCGG ATTGGCCCAA 
CAGCCCATCG AGGTAGAGAC CTCGTTGACC GTCAAGTCCT CTCTGACCAC CACGGAGGAG 
GTGCTGGTCT CGCGCAACGG ACAAATCCTA GACGAACGCA CCGTGGAGAC CAAGGAAAAC 
TTTAACCATC AGTCGCACCA GAAAGAGATC TGGCATCCTG TCAAGCAGCA GTCGGCCTTG 
GCGCGTGCCA CTCCCGAGAA TGCCCTCAAA TACCGAACCA CTTCGAATTC CACGTTCGCA 
TCGCAGGCCA CAGACTCGGA CGAGCGTCCA GTTGATCCTT TGGGCCGGCA GCTGAACCAA 
ACCTTCTCGA ATGCTTCCCT TATGTCCAGC AGCTCTGCTA TTACTGGATC CGATCAGGTC 
ATCGGATCTG TATCATCCAC CACTATTGGG GACAAAAGCT CGAATATAAG CAGCTGCGAC 
TCCGGGCACG ATGGCCTCTT CCACAGCGTG TCGATGAGTG CACCCTGGTC ACATCCACGT 
GACACCTTAA CCGACTCTTT GTACTGCGAC ATTCCCAGCT CCGCAGATGA GTCGGATGCG 
GTTTCTATCC TAAACACCCA CTTGAAGATC ACAGAGCCAT TCGATTGCCA AACAGCGCCA 
GTTTACGCCA AAGACATACC CAAGAAGGGC AACCTGGATT CGGTGGTCAA CCTGCTTATC 
AATAATAATA CTAAGGATTT GCGGTTTGCC TTTCTGACCA CTTCCCGCTT GTTTTCACCA 
CCACACCAGC TGCTTTCCAA AATCATTGCC AAGATTGATA ATGAGGAGGA AAACGTGTTA 
CGACTTTTGA ACGAATGGCT AGACACCTGT CCCGGGGACT TTAGCCATGA ACTGCTATTG 
CAGGAGCTGG AAAATAAGCG TAATGTAAAA GGATTGGCAG CTTTTTTGGA TGGAATTCCC 
CAAGCTTTGG CGCAAAAGGA CGAAAATGGA CGAAGTCAAC TAAATAACCT GCTTACCAAG 
CAATCGTCGG CCAGCTCGTT GGGACGTCAG AGCAGTATTA AGTCGCTGAA ACGCTTTGCC 
GCCAATGTCT ACAAAACAGA CAATGTGCTA AACAACTGCA GCAGTGCCTT CGAGTTGGCT 
CACCAACTTT ATGCCATCGA GTACGCCTAT CTGTCACAAA TACGTCTTGA GGAGTTTGTG 
GAAATCCTCG AAAAGGATGA GCTGAAGACC TGCATAAGCC AAACCAAGGC GGGCACCTTG 
GGTTCGAATT GCATCAGCCA GGTGACAATC GACAGTTACG TGCAGTGGTT CAACCAGCTA 
AGCTACTTGA CTGCAACGGA AATATTAAAG TTGGGCAAAA AGTCCCAGAG AGCACAAATG 
ATCGACTTCT GGGTGGAAAC AGCTCTGGAA TGCTTTAACA CAGGCAACTT CAACAGCTTG 
ATGGCCATTC TAACAGCATT GAATCTTACT GCCATAGCTC GCCTAAAGAA AACGTGGGCC 
AAAGTGCAGA CGACAAAGTT CGAGGGTTTG GAGCATCAAA TGGATCCCAG TTCGAACTTT 
CTTAACTACA GATCCACCAT GAAGGCAGCT GTTTGGCGTT CGGAAAGGGA AATGGCCAAC 
GAAATTGAGC GTGCCATCAT CCCATTCTTC TCGCTGTTCC TCAAAGATTT GCATGCCATT 
AACGAGAGCC ATGAAACAAA ATTGGCCAAC GGATACATAA ACTTTGAGAA GTGCCTTCAT 
TTGGGCACCC AGCTGCGGAA TTTTGGCAAA TGGCAGCGAT TGGACTGTCC ATACGAGCAG 
TTGCCTAGTG TGGTGTCCTA CCTGTTGAAG GCGGAGGTGC TGAGCGAGGA TCTTTTGATG 
AAGGCCTCCT ACGAAAGCGA GCCACCCGAG ACCGGCGAGG AGAAGGATCA CTACAAGACG 
TTGAAAGCTG CAAGGAAATC GAACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG4853 ( NM_001299606.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286535.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG4853), mRNA.

Price & Availability↑

CloneID	OFa21842
Clone ID Related Accession (Same CDS sequence)	NM_001299606.1
Accession Version	NM_001299606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGAAG ATATCCTAAA CGCTGTGGAG TCCCTGTTCG GCAAGGATGT CAAGATCGTG 
GACTGCGAAA CCTCATCCTC GCTGCAGCAG GAGGAGGTGT TGCTGGTCAA CGGAGTGCCG 
GTGAAGCTGG ATGGGCTGCC GGCGGATGAT GCCAGTGCCA TAAAGGCAGC TTTGCTGGAG 
GGGCAGATGC CATCGGTTGA GCACCTGAAT CAATTGCTCT TCCGCGCCGG ATTGGCCCAA 
CAGCCCATCG AGGTAGAGAC CTCGTTGACC GTCAAGTCCT CTCTGACCAC CACGGAGGAG 
GTGCTGGTCT CGCGCAACGG ACAAATCCTA GACGAACGCA CCGTGGAGAC CAAGGAAAAC 
TTTAACCATC AGTCGCACCA GAAAGAGATC TGGCATCCTG TCAAGCAGCA GTCGGCCTTG 
GCGCGTGCCA CTCCCGAGAA TGCCCTCAAA TACCGAACCA CTTCGAATTC CACGTTCGCA 
TCGCAGGCCA CAGACTCGGA CGAGCGTCCA GTTGATCCTT TGGGCCGGCA GCTGAACCAA 
ACCTTCTCGA ATGCTTCCCT TATGTCCAGC AGCTCTGCTA TTACTGGATC CGATCAGGTC 
ATCGGATCTG TATCATCCAC CACTATTGGG GACAAAAGCT CGAATATAAG CAGCTGCGAC 
TCCGGGCACG ATGGCCTCTT CCACAGCGTG TCGATGAGTG CACCCTGGTC ACATCCACGT 
GACACCTTAA CCGACTCTTT GTACTGCGAC ATTCCCAGCT CCGCAGATGA GTCGGATGCG 
GTTTCTATCC TAATCACAGA GCCATTCGAT TGCCAAACAG CGCCAGTTTA CGCCAAAGAC 
ATACCCAAGA AGGGCAACCT GGATTCGGTG GTCAACCTGC TTATCAATAA TAATACTAAG 
GATTTGCGGT TTGCCTTTCT GACCACTTCC CGCTTGTTTT CACCACCACA CCAGCTGCTT 
TCCAAAATCA TTGCCAAGAT TGATAATGAG GAGGAAAACG TGTTACGACT TTTGAACGAA 
TGGCTAGACA CCTGTCCCGG GGACTTTAGC CATGAACTGC TATTGCAGGA GCTGGAAAAT 
AAGCGTAATG TAAAAGGATT GGCAGCTTTT TTGGATGGAA TTCCCCAAGC TTTGGCGCAA 
AAGGACGAAA ATGGACGAAG TCAACTAAAT AACCTGCTTA CCAAGCAATC GTCGGCCAGC 
TCGTTGGGAC GTCAGAGCAG TATTAAGTCG CTGAAACGCT TTGCCGCCAA TGTCTACAAA 
ACAGACAATG TGCTAAACAA CTGCAGCAGT GCCTTCGAGT TGGCTCACCA ACTTTATGCC 
ATCGAGTACG CCTATCTGTC ACAAATACGT CTTGAGGAGT TTGTGGAAAT CCTCGAAAAG 
GATGAGCTGA AGACCTGCAT AAGCCAAACC AAGGCGGGCA CCTTGGGTTC GAATTGCATC 
AGCCAGGTGA CAATCGACAG TTACGTGCAG TGGTTCAACC AGCTAAGCTA CTTGACTGCA 
ACGGAAATAT TAAAGTTGGG CAAAAAGTCC CAGAGAGCAC AAATGATCGA CTTCTGGGTG 
GAAACAGCTC TGGAATGCTT TAACACAGGC AACTTCAACA GCTTGATGGC CATTCTAACA 
GCATTGAATC TTACTGCCAT AGCTCGCCTA AAGAAAACGT GGGCCAAAGT GCAGACGACA 
AAGTTCGAGG GTTTGGAGCA TCAAATGGAT CCCAGTTCGA ACTTTCTTAA CTACAGATCC 
ACCATGAAGG CAGCTGTTTG GCGTTCGGAA AGGGAAATGG CCAACGAAAT TGAGCGTGCC 
ATCATCCCAT TCTTCTCGCT GTTCCTCAAA GATTTGCATG CCATTAACGA GAGCCATGAA 
ACAAAATTGG CCAACGGATA CATAAACTTT GAGAAGTGCC TTCATTTGGG CACCCAGCTG 
CGGAATTTTG GCAAATGGCA GCGATTGGAC TGTCCATACG AGCAGTTGCC TAGTGTGGTG 
TCCTACCTGT TGAAGGCGGA GGTGCTGAGC GAGGATCTTT TGATGAAGGC CTCCTACGAA 
AGCGAGCCAC CCGAGACCGG CGAGGAGAAG GATCACTACA AGACGTTGAA AGCTGCAAGG 
AAATCGAACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286535.1
CDS	359..2473
Translation	MDEDILNAVESLFGKDVKIVDCETSSSLQQEEVLLVNGVPVKLDGLPADDASAIKAALLEGQMPSVEHLNQLLFRAGLAQQPIEVETSLTVKSSLTTTEEVLVSRNGQILDERTVETKENFNHQSHQKEIWHPVKQQSALARATPENALKYRTTSNSTFASQATDSDERPVDPLGRQLNQTFSNASLMSSSSAITGSDQVIGSVSSTTIGDKSSNISSCDSGHDGLFHSVSMSAPWSHPRDTLTDSLYCDIPSSADESDAVSILITEPFDCQTAPVYAKDIPKKGNLDSVVNLLINNNTKDLRFAFLTTSRLFSPPHQLLSKIIAKIDNEEENVLRLLNEWLDTCPGDFSHELLLQELENKRNVKGLAAFLDGIPQALAQKDENGRSQLNNLLTKQSSASSLGRQSSIKSLKRFAANVYKTDNVLNNCSSAFELAHQLYAIEYAYLSQIRLEEFVEILEKDELKTCISQTKAGTLGSNCISQVTIDSYVQWFNQLSYLTATEILKLGKKSQRAQMIDFWVETALECFNTGNFNSLMAILTALNLTAIARLKKTWAKVQTTKFEGLEHQMDPSSNFLNYRSTMKAAVWRSEREMANEIERAIIPFFSLFLKDLHAINESHETKLANGYINFEKCLHLGTQLRNFGKWQRLDCPYEQLPSVVSYLLKAEVLSEDLLMKASYESEPPETGEEKDHYKTLKAARKSNT

Target ORF information:

RefSeq Version	NM_001299606.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG4853), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299606.1

ORF Insert Sequence:

ATGGACGAAG ATATCCTAAA CGCTGTGGAG TCCCTGTTCG GCAAGGATGT CAAGATCGTG 
GACTGCGAAA CCTCATCCTC GCTGCAGCAG GAGGAGGTGT TGCTGGTCAA CGGAGTGCCG 
GTGAAGCTGG ATGGGCTGCC GGCGGATGAT GCCAGTGCCA TAAAGGCAGC TTTGCTGGAG 
GGGCAGATGC CATCGGTTGA GCACCTGAAT CAATTGCTCT TCCGCGCCGG ATTGGCCCAA 
CAGCCCATCG AGGTAGAGAC CTCGTTGACC GTCAAGTCCT CTCTGACCAC CACGGAGGAG 
GTGCTGGTCT CGCGCAACGG ACAAATCCTA GACGAACGCA CCGTGGAGAC CAAGGAAAAC 
TTTAACCATC AGTCGCACCA GAAAGAGATC TGGCATCCTG TCAAGCAGCA GTCGGCCTTG 
GCGCGTGCCA CTCCCGAGAA TGCCCTCAAA TACCGAACCA CTTCGAATTC CACGTTCGCA 
TCGCAGGCCA CAGACTCGGA CGAGCGTCCA GTTGATCCTT TGGGCCGGCA GCTGAACCAA 
ACCTTCTCGA ATGCTTCCCT TATGTCCAGC AGCTCTGCTA TTACTGGATC CGATCAGGTC 
ATCGGATCTG TATCATCCAC CACTATTGGG GACAAAAGCT CGAATATAAG CAGCTGCGAC 
TCCGGGCACG ATGGCCTCTT CCACAGCGTG TCGATGAGTG CACCCTGGTC ACATCCACGT 
GACACCTTAA CCGACTCTTT GTACTGCGAC ATTCCCAGCT CCGCAGATGA GTCGGATGCG 
GTTTCTATCC TAATCACAGA GCCATTCGAT TGCCAAACAG CGCCAGTTTA CGCCAAAGAC 
ATACCCAAGA AGGGCAACCT GGATTCGGTG GTCAACCTGC TTATCAATAA TAATACTAAG 
GATTTGCGGT TTGCCTTTCT GACCACTTCC CGCTTGTTTT CACCACCACA CCAGCTGCTT 
TCCAAAATCA TTGCCAAGAT TGATAATGAG GAGGAAAACG TGTTACGACT TTTGAACGAA 
TGGCTAGACA CCTGTCCCGG GGACTTTAGC CATGAACTGC TATTGCAGGA GCTGGAAAAT 
AAGCGTAATG TAAAAGGATT GGCAGCTTTT TTGGATGGAA TTCCCCAAGC TTTGGCGCAA 
AAGGACGAAA ATGGACGAAG TCAACTAAAT AACCTGCTTA CCAAGCAATC GTCGGCCAGC 
TCGTTGGGAC GTCAGAGCAG TATTAAGTCG CTGAAACGCT TTGCCGCCAA TGTCTACAAA 
ACAGACAATG TGCTAAACAA CTGCAGCAGT GCCTTCGAGT TGGCTCACCA ACTTTATGCC 
ATCGAGTACG CCTATCTGTC ACAAATACGT CTTGAGGAGT TTGTGGAAAT CCTCGAAAAG 
GATGAGCTGA AGACCTGCAT AAGCCAAACC AAGGCGGGCA CCTTGGGTTC GAATTGCATC 
AGCCAGGTGA CAATCGACAG TTACGTGCAG TGGTTCAACC AGCTAAGCTA CTTGACTGCA 
ACGGAAATAT TAAAGTTGGG CAAAAAGTCC CAGAGAGCAC AAATGATCGA CTTCTGGGTG 
GAAACAGCTC TGGAATGCTT TAACACAGGC AACTTCAACA GCTTGATGGC CATTCTAACA 
GCATTGAATC TTACTGCCAT AGCTCGCCTA AAGAAAACGT GGGCCAAAGT GCAGACGACA 
AAGTTCGAGG GTTTGGAGCA TCAAATGGAT CCCAGTTCGA ACTTTCTTAA CTACAGATCC 
ACCATGAAGG CAGCTGTTTG GCGTTCGGAA AGGGAAATGG CCAACGAAAT TGAGCGTGCC 
ATCATCCCAT TCTTCTCGCT GTTCCTCAAA GATTTGCATG CCATTAACGA GAGCCATGAA 
ACAAAATTGG CCAACGGATA CATAAACTTT GAGAAGTGCC TTCATTTGGG CACCCAGCTG 
CGGAATTTTG GCAAATGGCA GCGATTGGAC TGTCCATACG AGCAGTTGCC TAGTGTGGTG 
TCCTACCTGT TGAAGGCGGA GGTGCTGAGC GAGGATCTTT TGATGAAGGC CTCCTACGAA 
AGCGAGCCAC CCGAGACCGG CGAGGAGAAG GATCACTACA AGACGTTGAA AGCTGCAAGG 
AAATCGAACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG4853 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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