cnk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cnk
Entrez Gene ID	36952
Full Name	connector enhancer of ksr
Synonyms	CG6556,CNK,Cnk,Dmel\CG6556,EC2-3,EK2-3,anon-WO0140519.129,anon-WO0257455.27,l(2)k16314
General protein information	Preferred Names connector enhancer of ksr Names CG6556 gene product from transcript CG6556-RB CG6556-PA CG6556-PB cnk-PA cnk-PB connector enhancer of KSR enhancer of KDN
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54B7-54B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299595.1	NP_001286524.1	connector enhancer of ksr, isoform B
NM_166223.2	NP_725671.1	connector enhancer of ksr, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009167	XP_319930.4
Drosophila melanogaster (fruit fly)	cnk	NP_725671.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Review highlights the implication of CNK and KSR in a novel mechanism regulating RAS-mediated RAF activation.
Title: KSR and CNK: two scaffolds regulating RAS-mediated RAF activation.

the integration of RTK-induced RAS and Src42 signals by CNK as a two-component input is essential for RAF activation
Title: Src42 binding activity regulates Drosophila RAF by a novel CNK-dependent derepression mechanism.

The following cnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13024	NM_001299595.1 Latest version!	Drosophila melanogaster connector enhancer of ksr (cnk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa13024	NM_166223.2 Latest version!	Drosophila melanogaster connector enhancer of ksr (cnk), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cnk ( NM_001299595.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286524.1 Drosophila melanogaster connector enhancer of ksr (cnk), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13024
Clone ID Related Accession (Same CDS sequence)	NM_001299595.1 , NM_166223.2
Accession Version	NM_001299595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	connector enhancer of ksr, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTACA TCAACATCGC CGAGTGGACG CCCGACCAGG TCACCGACTG GATAAAAGGT 
CTGGATGAGT CTATGAAGGG GTATTTGTAC GAGTTCTCCA AGCAGGAGAT CGGCGGACGG 
GCGTTGCTCA ACATACGGCC ATACGAGCTG GAGAATCTGG GCATGCTGCG CATTGGCCAT 
CAGGAGATCG TGCTGGAGGC GGTGGAGAAC TTAAGAAACT TCCATTATCA CCTGAAAAAC 
GACAATCTGC AGTTCATGGC CTTGCATGTG GCCACGGCAG CTAAGAATCT GCATCGCGAA 
CTGGCCAGGA ATCATGCGGA GAGCACTAAG ATCGATACCC GGATACTGCA CGATATTACC 
AGGACGATAG CTACTCTAAA GCCATTGGTG GGCAGCCTGG AGCGAACACC GTTCCGTAAG 
CAGGAGATGT ACCGCGAGTA TTGTGGCAAT GTGCTCAAAT GCGGCCTGGA GCTGGCCACA 
ATTGCCCACC GTGATCGTTT TGCCCTGCAG CCGGTGCCAG CGATTCGACA GTCAGCGGAA 
CGGCTCGAGA ATCTTGCCAA CTTTGTAATC CAGGACATAT CTGATCCCAT GGTACTGCAG 
CCAGCCTCTC TGAACCTTGT CACCCTTAAA AAACGCGAAT CCGAGTTGGG TTTCAACATA 
GAGTCCAGTT ATAATGGCAT TCACCGTGTC ACGGATATCA AGTACAACTC GCCAGCTCAC 
AATTCCGGAA AAATCGAAGA TGGCGACGAG ATTGTGCAGA TCAACTACCA GACCGTTGTG 
GGCTGGCAGC ATCGCACAGT ACTGGAGCAT CTGAGGGAAG CACTTCCAGA TGTGGTGCTG 
ACGGTGAAGA AACGGCCGAA GCACACAAAG ATGTTTGGAC AGATCTATAT GCAGCCGTAC 
AGGTTGCCCA GCAAGAAGAG GAATATGGCC GCCCGCTGGG CGGCACAGAT GCCAAGTCCC 
CGTGCTGCAT TTTTAACCCT GGACACGGAG CAACTGGCAA CAGGAGGAAC AGGAAGCGCG 
GTGCCAGATC CCAGTAAATC CCTAAGCAGT GAGAAGAGGG AGGTACTTAC CAAGGCCAAT 
CCAGTGTCCT CTGCCTCCGA CTCTGATTCC AGTTGCAGTG ATATTCCAAC ACCAACGGAT 
CCCAAGCTAG CCGCACGGGA GATCCGTTTG TATTACCCAA AGCCAAGAGC TTTGCTCCAA 
CGAAGAAACA CTATTTGTGG ATGCGAGTAT CTTAGCCTGA AAAACTCGGA TCTAGTCGTT 
CCATCGTGGC ACGAAAGAAA GCCAGGAATT GGTTCGCCCA CCAATTGCGA CCCTGGCTCG 
CCCAGTATAC GGGATAAGTC GATATCCTTC GGTTATGGCC TAGAGATGGC AGCAAGGCCG 
ACCACTTGCA TTGGGATTGC GGGCGATACC TCCACGGACA AGGCCAGGCG AATGTTTCAT 
GAGGCGCGAA AACTAAAGCA GCTGACGGAG GACTCGCAGC GCGAATTCCT CGTGGATCGT 
TACAAGCCGG GTGTCAGCAA AGTAGTTCGG TTCGACGCCA AGGAAGATTA TGTGATGAAG 
AATGAAAAAT TTATCTGTAA CGTTGAGAAC ACCATACTGG AGACCTTTGA ACCGATTCCC 
TTTGCGGATG AGGGAGACGA GGACGCCTTG GAAACGCTGC GCAACTGCAA AACAGAAAAC 
GCAGAGGAGC TCCTAGAGGC AATTAATCTG GCTACAAAAG GTCAGGACTT GCCCCTGGCC 
GAGGCCATTA ACATGCCTTT GTTGCAGCAA GGTCGAAGGG GCCGATTAGA CAAGAGTCAT 
AGTACTCCGG CATACGATAA TTCGGGTGAA GAATCGGATA CGCCGCCAGC GATTGAGCCA 
AGAAAGGAGT TCCTGCTTGT CACACCGCCA GCACCGCCAC CGCGACCCCG CAAGCAAAGG 
GAAATGACGC CACCGGCGGT GCCTCCACCT CCACCAAAAC CAGCCAGCAT GCAGCCGGCT 
AGTAGTATCA CCAGTATCTC CATTCCCGTG CCAGTACCTG TACCAGCCGC AGTTGATCCA 
TCGGAGATCA GTGAGCTTCA TACGCCCAGC AAATCCCGGA CACTGACCCT TAAAAAGAAG 
CACAGCTTAA TGGCCAAGCG GCGGAACACA AATCTCAAGC TGTTGGGGAC TGGGGATATC 
CAAGGTCATC TGTACAGGCG CAAGAAAAAT CATCGCGGAG TGACTTACTG GGCGAGAATC 
TATTTCGTGA TGCTGGATAC GATACTGTAC GGATTTCGCA GTAAGCAGAG TACCAGCGCC 
AGCTTGGTAA TATTTTTACC GGGCTTTACG GTTTCATTGG CAAAAGAAGT GCACTCCAAA 
CCGCATGCCT TTAAAGTTTA CCACACGGCC AAGAGCTTCT ACTTTGCGGC CGAGTCGCTG 
GACGCACTTA ACCAATGGGT GGACTTCTTG CGCCAGGCTT CGCTCAAAGT GCCGCCTAGT 
ACGGGTTCAA AAGGCGGTGG TGACGCAAAG GATTTGTATT CGGAGAACGA TAGCTCAGGC 
GAGGAATGCG ATGCCCTTGT GATCCAGAAT TTAAGCACGC CCTCGCCGCA AGGCAACAAG 
GAGTCAATGT CCATGTCGAT GACACTATCT GGCGGAACAC CACCTTCCTC TGCACCCATT 
AAGCATGAAC GCGGCTACTT GGACTCGTTT CGCAAATTTA CGAATACATT CAAGAGCAGT 
GCAGCGAAAC CGTCGAGCGA CATCCCTGTG CCAACGGAGC AATACCGCAG CTATAGGAAG 
GTGCCAGGCG GAAGTTTTGG CATTCAGATT GGTGCCAATA CACCGGGCTA CCATGATCCC 
GCAATGCCGC CGACACAGAT TCCACCTTTG GTGGGTCCTA AACTGTCGCG TAGCTCAAGT 
AGGAGTTCGG TGGTCAGTGG AACCGAGTCT GCCGTATCAT TATCTGCTTC GATTCTGGGT 
CCGCCCGCGT CGCCAGCGCC AACGCCCACG CCTACAGCCA CTCCCACATC GCTGCAGCAT 
CAGAGCTCCG AGGAGAGTCC AAGTCAGAGC CAGAGCCAGA GTCCCAGTAG CAAGTCGAGT 
TTGAAGAAGG CACCATTCAA CTTCTTGCAC GCCTCAAATC CGAATTTGGT GGAATTCGAT 
TTCCATACCT CGAAAACACT ACTCCCCAAG ATGAGTGTGG GTAATACACT CGACCATGGC 
CATAATATAC AGGGCTTTGT GACGCTTAAG GATCTGATGC TGCGCAAACA GGAGGAGGAG 
GCCCAGGAAA TGTACAACAA TCGAGTGCAC TTGGGTGTGG AGAAGCACAA GCACGCACGG 
ACGGAATCCA CGGCCAGTCA GCAGAGCGCC ATGAGCAGCA GCGTGACAAA GCCGCTGGAA 
AAGTTGCCCA AGATCCAGAG CGTGAGTCTG CCCAAAACAC CCGACTACGA GATCAGTTTT 
AAGCCTGACG ACGAGAGTAT TAAGAGAACC AGAACTAAGG AAGGTCAGAA GTTGCGCGAC 
TTTGGTTACG AGCTGATATG TGGGGATGAG CCGAGCACTA GCTCCATCAG CCGGCAAGAG 
CACCACCATC ATCATCATCA TCACCAGCAC CACCTGCAGC ATGTGCAACA GCAGCAGCAG 
CTACAACATC AGCAGACGCA GCAGCAACAT AGCAGCAAGG CCAAGCACTT CTTGCGCTCA 
CAGCAGTTAC AGCTCTCCAG TTTCCTGCAC AAGAACAGCA GCGGCGGCAG CGGCAAGAGT 
TCCGGATCCG GTGCGGATCA AAAGAAGTCC AAAGGAAAGT CTAGCGGCGA TCGTCGGTTT 
CCCTTTTCCA AGCACTCAAC TGGCTCCGCG GGCAGTGGCT GTGGGCCGGG TCATGCGATG 
ACTATGACGC TGCCGCTGAA CAAGAAGTCC AAATCGAATC ACGCCTTGGA TGGAGCCGTA 
GGCAATGGAG TCACCATAAT CGGCAGCGGC AGCAGCATCA AGAAAAGCCA GACGTACAAT 
CAGGATCTGC GAGACAAGAT AGTGGGCACC AAGTACGATG CGCATCGCAA GAACTCGGCC 
CCGATACCAA TCTTTTCGAA ACTCTCCATA TCGGGTGGCA CGCCGGCGAA GCCCTCCAAG 
GAGAATCGTT TCCTGGGCTC ACCGCTGCTG CATCGCACGC TGTTCGGGCA CCATCACCAT 
CAGCAGCAGG CAGTAACGCC ACCAAGCAGT GCGGATCCCG ATTGCGATCA GGAGATCTTC 
TCCCAAATCA CCCTGCCCAC GCACAATCAG GCGGGATATC GAAGTTACCG TGGACCTGGC 
ATACTAACCA CCTCGACCAC TAATTTGTGC TCGTCGGAGG GCTTGCAGCC GCCGTTGCCC 
CCAGCGCCAC CGCCTCCCGT CAACACCAGC AGGCAGAGAG AGGAGGAGCA AGCGGAGGCA 
ACGATATCAG AGTGTGCGGC CACCCCAAAA GTGTCCAGTT CGGGATCTGT GGATTCGAAG 
GCGGCAACGC CGGACTATCC AAATATGGAG TGTCCGCCTG TCTTTGAGCC GGAAATCTAC 
TCGCTCAGCG ATACCAGCTT GTCCCGCATC GTGATGCGGA CGCCCAGCAG CAGTGGTGCC 
ACCAGTGCCA CCGCCAGCAC CACCACCACC AACAACAACA ACAACAATAA TAACACCAAT 
AACAACACCA ACACCAACAG CAGTCCCAGT GGCGGCGAAC ACCATCAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286524.1
CDS	540..5213
Translation	MAYINIAEWTPDQVTDWIKGLDESMKGYLYEFSKQEIGGRALLNIRPYELENLGMLRIGHQEIVLEAVENLRNFHYHLKNDNLQFMALHVATAAKNLHRELARNHAESTKIDTRILHDITRTIATLKPLVGSLERTPFRKQEMYREYCGNVLKCGLELATIAHRDRFALQPVPAIRQSAERLENLANFVIQDISDPMVLQPASLNLVTLKKRESELGFNIESSYNGIHRVTDIKYNSPAHNSGKIEDGDEIVQINYQTVVGWQHRTVLEHLREALPDVVLTVKKRPKHTKMFGQIYMQPYRLPSKKRNMAARWAAQMPSPRAAFLTLDTEQLATGGTGSAVPDPSKSLSSEKREVLTKANPVSSASDSDSSCSDIPTPTDPKLAAREIRLYYPKPRALLQRRNTICGCEYLSLKNSDLVVPSWHERKPGIGSPTNCDPGSPSIRDKSISFGYGLEMAARPTTCIGIAGDTSTDKARRMFHEARKLKQLTEDSQREFLVDRYKPGVSKVVRFDAKEDYVMKNEKFICNVENTILETFEPIPFADEGDEDALETLRNCKTENAEELLEAINLATKGQDLPLAEAINMPLLQQGRRGRLDKSHSTPAYDNSGEESDTPPAIEPRKEFLLVTPPAPPPRPRKQREMTPPAVPPPPPKPASMQPASSITSISIPVPVPVPAAVDPSEISELHTPSKSRTLTLKKKHSLMAKRRNTNLKLLGTGDIQGHLYRRKKNHRGVTYWARIYFVMLDTILYGFRSKQSTSASLVIFLPGFTVSLAKEVHSKPHAFKVYHTAKSFYFAAESLDALNQWVDFLRQASLKVPPSTGSKGGGDAKDLYSENDSSGEECDALVIQNLSTPSPQGNKESMSMSMTLSGGTPPSSAPIKHERGYLDSFRKFTNTFKSSAAKPSSDIPVPTEQYRSYRKVPGGSFGIQIGANTPGYHDPAMPPTQIPPLVGPKLSRSSSRSSVVSGTESAVSLSASILGPPASPAPTPTPTATPTSLQHQSSEESPSQSQSQSPSSKSSLKKAPFNFLHASNPNLVEFDFHTSKTLLPKMSVGNTLDHGHNIQGFVTLKDLMLRKQEEEAQEMYNNRVHLGVEKHKHARTESTASQQSAMSSSVTKPLEKLPKIQSVSLPKTPDYEISFKPDDESIKRTRTKEGQKLRDFGYELICGDEPSTSSISRQEHHHHHHHHQHHLQHVQQQQQLQHQQTQQQHSSKAKHFLRSQQLQLSSFLHKNSSGGSGKSSGSGADQKKSKGKSSGDRRFPFSKHSTGSAGSGCGPGHAMTMTLPLNKKSKSNHALDGAVGNGVTIIGSGSSIKKSQTYNQDLRDKIVGTKYDAHRKNSAPIPIFSKLSISGGTPAKPSKENRFLGSPLLHRTLFGHHHHQQQAVTPPSSADPDCDQEIFSQITLPTHNQAGYRSYRGPGILTTSTTNLCSSEGLQPPLPPAPPPPVNTSRQREEEQAEATISECAATPKVSSSGSVDSKAATPDYPNMECPPVFEPEIYSLSDTSLSRIVMRTPSSSGATSATASTTTTNNNNNNNNTNNNTNTNSSPSGGEHHQT

Target ORF information:

RefSeq Version	NM_001299595.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster connector enhancer of ksr (cnk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299595.1

ORF Insert Sequence:

ATGGCCTACA TCAACATCGC CGAGTGGACG CCCGACCAGG TCACCGACTG GATAAAAGGT 
CTGGATGAGT CTATGAAGGG GTATTTGTAC GAGTTCTCCA AGCAGGAGAT CGGCGGACGG 
GCGTTGCTCA ACATACGGCC ATACGAGCTG GAGAATCTGG GCATGCTGCG CATTGGCCAT 
CAGGAGATCG TGCTGGAGGC GGTGGAGAAC TTAAGAAACT TCCATTATCA CCTGAAAAAC 
GACAATCTGC AGTTCATGGC CTTGCATGTG GCCACGGCAG CTAAGAATCT GCATCGCGAA 
CTGGCCAGGA ATCATGCGGA GAGCACTAAG ATCGATACCC GGATACTGCA CGATATTACC 
AGGACGATAG CTACTCTAAA GCCATTGGTG GGCAGCCTGG AGCGAACACC GTTCCGTAAG 
CAGGAGATGT ACCGCGAGTA TTGTGGCAAT GTGCTCAAAT GCGGCCTGGA GCTGGCCACA 
ATTGCCCACC GTGATCGTTT TGCCCTGCAG CCGGTGCCAG CGATTCGACA GTCAGCGGAA 
CGGCTCGAGA ATCTTGCCAA CTTTGTAATC CAGGACATAT CTGATCCCAT GGTACTGCAG 
CCAGCCTCTC TGAACCTTGT CACCCTTAAA AAACGCGAAT CCGAGTTGGG TTTCAACATA 
GAGTCCAGTT ATAATGGCAT TCACCGTGTC ACGGATATCA AGTACAACTC GCCAGCTCAC 
AATTCCGGAA AAATCGAAGA TGGCGACGAG ATTGTGCAGA TCAACTACCA GACCGTTGTG 
GGCTGGCAGC ATCGCACAGT ACTGGAGCAT CTGAGGGAAG CACTTCCAGA TGTGGTGCTG 
ACGGTGAAGA AACGGCCGAA GCACACAAAG ATGTTTGGAC AGATCTATAT GCAGCCGTAC 
AGGTTGCCCA GCAAGAAGAG GAATATGGCC GCCCGCTGGG CGGCACAGAT GCCAAGTCCC 
CGTGCTGCAT TTTTAACCCT GGACACGGAG CAACTGGCAA CAGGAGGAAC AGGAAGCGCG 
GTGCCAGATC CCAGTAAATC CCTAAGCAGT GAGAAGAGGG AGGTACTTAC CAAGGCCAAT 
CCAGTGTCCT CTGCCTCCGA CTCTGATTCC AGTTGCAGTG ATATTCCAAC ACCAACGGAT 
CCCAAGCTAG CCGCACGGGA GATCCGTTTG TATTACCCAA AGCCAAGAGC TTTGCTCCAA 
CGAAGAAACA CTATTTGTGG ATGCGAGTAT CTTAGCCTGA AAAACTCGGA TCTAGTCGTT 
CCATCGTGGC ACGAAAGAAA GCCAGGAATT GGTTCGCCCA CCAATTGCGA CCCTGGCTCG 
CCCAGTATAC GGGATAAGTC GATATCCTTC GGTTATGGCC TAGAGATGGC AGCAAGGCCG 
ACCACTTGCA TTGGGATTGC GGGCGATACC TCCACGGACA AGGCCAGGCG AATGTTTCAT 
GAGGCGCGAA AACTAAAGCA GCTGACGGAG GACTCGCAGC GCGAATTCCT CGTGGATCGT 
TACAAGCCGG GTGTCAGCAA AGTAGTTCGG TTCGACGCCA AGGAAGATTA TGTGATGAAG 
AATGAAAAAT TTATCTGTAA CGTTGAGAAC ACCATACTGG AGACCTTTGA ACCGATTCCC 
TTTGCGGATG AGGGAGACGA GGACGCCTTG GAAACGCTGC GCAACTGCAA AACAGAAAAC 
GCAGAGGAGC TCCTAGAGGC AATTAATCTG GCTACAAAAG GTCAGGACTT GCCCCTGGCC 
GAGGCCATTA ACATGCCTTT GTTGCAGCAA GGTCGAAGGG GCCGATTAGA CAAGAGTCAT 
AGTACTCCGG CATACGATAA TTCGGGTGAA GAATCGGATA CGCCGCCAGC GATTGAGCCA 
AGAAAGGAGT TCCTGCTTGT CACACCGCCA GCACCGCCAC CGCGACCCCG CAAGCAAAGG 
GAAATGACGC CACCGGCGGT GCCTCCACCT CCACCAAAAC CAGCCAGCAT GCAGCCGGCT 
AGTAGTATCA CCAGTATCTC CATTCCCGTG CCAGTACCTG TACCAGCCGC AGTTGATCCA 
TCGGAGATCA GTGAGCTTCA TACGCCCAGC AAATCCCGGA CACTGACCCT TAAAAAGAAG 
CACAGCTTAA TGGCCAAGCG GCGGAACACA AATCTCAAGC TGTTGGGGAC TGGGGATATC 
CAAGGTCATC TGTACAGGCG CAAGAAAAAT CATCGCGGAG TGACTTACTG GGCGAGAATC 
TATTTCGTGA TGCTGGATAC GATACTGTAC GGATTTCGCA GTAAGCAGAG TACCAGCGCC 
AGCTTGGTAA TATTTTTACC GGGCTTTACG GTTTCATTGG CAAAAGAAGT GCACTCCAAA 
CCGCATGCCT TTAAAGTTTA CCACACGGCC AAGAGCTTCT ACTTTGCGGC CGAGTCGCTG 
GACGCACTTA ACCAATGGGT GGACTTCTTG CGCCAGGCTT CGCTCAAAGT GCCGCCTAGT 
ACGGGTTCAA AAGGCGGTGG TGACGCAAAG GATTTGTATT CGGAGAACGA TAGCTCAGGC 
GAGGAATGCG ATGCCCTTGT GATCCAGAAT TTAAGCACGC CCTCGCCGCA AGGCAACAAG 
GAGTCAATGT CCATGTCGAT GACACTATCT GGCGGAACAC CACCTTCCTC TGCACCCATT 
AAGCATGAAC GCGGCTACTT GGACTCGTTT CGCAAATTTA CGAATACATT CAAGAGCAGT 
GCAGCGAAAC CGTCGAGCGA CATCCCTGTG CCAACGGAGC AATACCGCAG CTATAGGAAG 
GTGCCAGGCG GAAGTTTTGG CATTCAGATT GGTGCCAATA CACCGGGCTA CCATGATCCC 
GCAATGCCGC CGACACAGAT TCCACCTTTG GTGGGTCCTA AACTGTCGCG TAGCTCAAGT 
AGGAGTTCGG TGGTCAGTGG AACCGAGTCT GCCGTATCAT TATCTGCTTC GATTCTGGGT 
CCGCCCGCGT CGCCAGCGCC AACGCCCACG CCTACAGCCA CTCCCACATC GCTGCAGCAT 
CAGAGCTCCG AGGAGAGTCC AAGTCAGAGC CAGAGCCAGA GTCCCAGTAG CAAGTCGAGT 
TTGAAGAAGG CACCATTCAA CTTCTTGCAC GCCTCAAATC CGAATTTGGT GGAATTCGAT 
TTCCATACCT CGAAAACACT ACTCCCCAAG ATGAGTGTGG GTAATACACT CGACCATGGC 
CATAATATAC AGGGCTTTGT GACGCTTAAG GATCTGATGC TGCGCAAACA GGAGGAGGAG 
GCCCAGGAAA TGTACAACAA TCGAGTGCAC TTGGGTGTGG AGAAGCACAA GCACGCACGG 
ACGGAATCCA CGGCCAGTCA GCAGAGCGCC ATGAGCAGCA GCGTGACAAA GCCGCTGGAA 
AAGTTGCCCA AGATCCAGAG CGTGAGTCTG CCCAAAACAC CCGACTACGA GATCAGTTTT 
AAGCCTGACG ACGAGAGTAT TAAGAGAACC AGAACTAAGG AAGGTCAGAA GTTGCGCGAC 
TTTGGTTACG AGCTGATATG TGGGGATGAG CCGAGCACTA GCTCCATCAG CCGGCAAGAG 
CACCACCATC ATCATCATCA TCACCAGCAC CACCTGCAGC ATGTGCAACA GCAGCAGCAG 
CTACAACATC AGCAGACGCA GCAGCAACAT AGCAGCAAGG CCAAGCACTT CTTGCGCTCA 
CAGCAGTTAC AGCTCTCCAG TTTCCTGCAC AAGAACAGCA GCGGCGGCAG CGGCAAGAGT 
TCCGGATCCG GTGCGGATCA AAAGAAGTCC AAAGGAAAGT CTAGCGGCGA TCGTCGGTTT 
CCCTTTTCCA AGCACTCAAC TGGCTCCGCG GGCAGTGGCT GTGGGCCGGG TCATGCGATG 
ACTATGACGC TGCCGCTGAA CAAGAAGTCC AAATCGAATC ACGCCTTGGA TGGAGCCGTA 
GGCAATGGAG TCACCATAAT CGGCAGCGGC AGCAGCATCA AGAAAAGCCA GACGTACAAT 
CAGGATCTGC GAGACAAGAT AGTGGGCACC AAGTACGATG CGCATCGCAA GAACTCGGCC 
CCGATACCAA TCTTTTCGAA ACTCTCCATA TCGGGTGGCA CGCCGGCGAA GCCCTCCAAG 
GAGAATCGTT TCCTGGGCTC ACCGCTGCTG CATCGCACGC TGTTCGGGCA CCATCACCAT 
CAGCAGCAGG CAGTAACGCC ACCAAGCAGT GCGGATCCCG ATTGCGATCA GGAGATCTTC 
TCCCAAATCA CCCTGCCCAC GCACAATCAG GCGGGATATC GAAGTTACCG TGGACCTGGC 
ATACTAACCA CCTCGACCAC TAATTTGTGC TCGTCGGAGG GCTTGCAGCC GCCGTTGCCC 
CCAGCGCCAC CGCCTCCCGT CAACACCAGC AGGCAGAGAG AGGAGGAGCA AGCGGAGGCA 
ACGATATCAG AGTGTGCGGC CACCCCAAAA GTGTCCAGTT CGGGATCTGT GGATTCGAAG 
GCGGCAACGC CGGACTATCC AAATATGGAG TGTCCGCCTG TCTTTGAGCC GGAAATCTAC 
TCGCTCAGCG ATACCAGCTT GTCCCGCATC GTGATGCGGA CGCCCAGCAG CAGTGGTGCC 
ACCAGTGCCA CCGCCAGCAC CACCACCACC AACAACAACA ACAACAATAA TAACACCAAT 
AACAACACCA ACACCAACAG CAGTCCCAGT GGCGGCGAAC ACCATCAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cnk ( NM_166223.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725671.1 Drosophila melanogaster connector enhancer of ksr (cnk), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13024
Clone ID Related Accession (Same CDS sequence)	NM_001299595.1 , NM_166223.2
Accession Version	NM_166223.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	connector enhancer of ksr, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166223.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTACA TCAACATCGC CGAGTGGACG CCCGACCAGG TCACCGACTG GATAAAAGGT 
CTGGATGAGT CTATGAAGGG GTATTTGTAC GAGTTCTCCA AGCAGGAGAT CGGCGGACGG 
GCGTTGCTCA ACATACGGCC ATACGAGCTG GAGAATCTGG GCATGCTGCG CATTGGCCAT 
CAGGAGATCG TGCTGGAGGC GGTGGAGAAC TTAAGAAACT TCCATTATCA CCTGAAAAAC 
GACAATCTGC AGTTCATGGC CTTGCATGTG GCCACGGCAG CTAAGAATCT GCATCGCGAA 
CTGGCCAGGA ATCATGCGGA GAGCACTAAG ATCGATACCC GGATACTGCA CGATATTACC 
AGGACGATAG CTACTCTAAA GCCATTGGTG GGCAGCCTGG AGCGAACACC GTTCCGTAAG 
CAGGAGATGT ACCGCGAGTA TTGTGGCAAT GTGCTCAAAT GCGGCCTGGA GCTGGCCACA 
ATTGCCCACC GTGATCGTTT TGCCCTGCAG CCGGTGCCAG CGATTCGACA GTCAGCGGAA 
CGGCTCGAGA ATCTTGCCAA CTTTGTAATC CAGGACATAT CTGATCCCAT GGTACTGCAG 
CCAGCCTCTC TGAACCTTGT CACCCTTAAA AAACGCGAAT CCGAGTTGGG TTTCAACATA 
GAGTCCAGTT ATAATGGCAT TCACCGTGTC ACGGATATCA AGTACAACTC GCCAGCTCAC 
AATTCCGGAA AAATCGAAGA TGGCGACGAG ATTGTGCAGA TCAACTACCA GACCGTTGTG 
GGCTGGCAGC ATCGCACAGT ACTGGAGCAT CTGAGGGAAG CACTTCCAGA TGTGGTGCTG 
ACGGTGAAGA AACGGCCGAA GCACACAAAG ATGTTTGGAC AGATCTATAT GCAGCCGTAC 
AGGTTGCCCA GCAAGAAGAG GAATATGGCC GCCCGCTGGG CGGCACAGAT GCCAAGTCCC 
CGTGCTGCAT TTTTAACCCT GGACACGGAG CAACTGGCAA CAGGAGGAAC AGGAAGCGCG 
GTGCCAGATC CCAGTAAATC CCTAAGCAGT GAGAAGAGGG AGGTACTTAC CAAGGCCAAT 
CCAGTGTCCT CTGCCTCCGA CTCTGATTCC AGTTGCAGTG ATATTCCAAC ACCAACGGAT 
CCCAAGCTAG CCGCACGGGA GATCCGTTTG TATTACCCAA AGCCAAGAGC TTTGCTCCAA 
CGAAGAAACA CTATTTGTGG ATGCGAGTAT CTTAGCCTGA AAAACTCGGA TCTAGTCGTT 
CCATCGTGGC ACGAAAGAAA GCCAGGAATT GGTTCGCCCA CCAATTGCGA CCCTGGCTCG 
CCCAGTATAC GGGATAAGTC GATATCCTTC GGTTATGGCC TAGAGATGGC AGCAAGGCCG 
ACCACTTGCA TTGGGATTGC GGGCGATACC TCCACGGACA AGGCCAGGCG AATGTTTCAT 
GAGGCGCGAA AACTAAAGCA GCTGACGGAG GACTCGCAGC GCGAATTCCT CGTGGATCGT 
TACAAGCCGG GTGTCAGCAA AGTAGTTCGG TTCGACGCCA AGGAAGATTA TGTGATGAAG 
AATGAAAAAT TTATCTGTAA CGTTGAGAAC ACCATACTGG AGACCTTTGA ACCGATTCCC 
TTTGCGGATG AGGGAGACGA GGACGCCTTG GAAACGCTGC GCAACTGCAA AACAGAAAAC 
GCAGAGGAGC TCCTAGAGGC AATTAATCTG GCTACAAAAG GTCAGGACTT GCCCCTGGCC 
GAGGCCATTA ACATGCCTTT GTTGCAGCAA GGTCGAAGGG GCCGATTAGA CAAGAGTCAT 
AGTACTCCGG CATACGATAA TTCGGGTGAA GAATCGGATA CGCCGCCAGC GATTGAGCCA 
AGAAAGGAGT TCCTGCTTGT CACACCGCCA GCACCGCCAC CGCGACCCCG CAAGCAAAGG 
GAAATGACGC CACCGGCGGT GCCTCCACCT CCACCAAAAC CAGCCAGCAT GCAGCCGGCT 
AGTAGTATCA CCAGTATCTC CATTCCCGTG CCAGTACCTG TACCAGCCGC AGTTGATCCA 
TCGGAGATCA GTGAGCTTCA TACGCCCAGC AAATCCCGGA CACTGACCCT TAAAAAGAAG 
CACAGCTTAA TGGCCAAGCG GCGGAACACA AATCTCAAGC TGTTGGGGAC TGGGGATATC 
CAAGGTCATC TGTACAGGCG CAAGAAAAAT CATCGCGGAG TGACTTACTG GGCGAGAATC 
TATTTCGTGA TGCTGGATAC GATACTGTAC GGATTTCGCA GTAAGCAGAG TACCAGCGCC 
AGCTTGGTAA TATTTTTACC GGGCTTTACG GTTTCATTGG CAAAAGAAGT GCACTCCAAA 
CCGCATGCCT TTAAAGTTTA CCACACGGCC AAGAGCTTCT ACTTTGCGGC CGAGTCGCTG 
GACGCACTTA ACCAATGGGT GGACTTCTTG CGCCAGGCTT CGCTCAAAGT GCCGCCTAGT 
ACGGGTTCAA AAGGCGGTGG TGACGCAAAG GATTTGTATT CGGAGAACGA TAGCTCAGGC 
GAGGAATGCG ATGCCCTTGT GATCCAGAAT TTAAGCACGC CCTCGCCGCA AGGCAACAAG 
GAGTCAATGT CCATGTCGAT GACACTATCT GGCGGAACAC CACCTTCCTC TGCACCCATT 
AAGCATGAAC GCGGCTACTT GGACTCGTTT CGCAAATTTA CGAATACATT CAAGAGCAGT 
GCAGCGAAAC CGTCGAGCGA CATCCCTGTG CCAACGGAGC AATACCGCAG CTATAGGAAG 
GTGCCAGGCG GAAGTTTTGG CATTCAGATT GGTGCCAATA CACCGGGCTA CCATGATCCC 
GCAATGCCGC CGACACAGAT TCCACCTTTG GTGGGTCCTA AACTGTCGCG TAGCTCAAGT 
AGGAGTTCGG TGGTCAGTGG AACCGAGTCT GCCGTATCAT TATCTGCTTC GATTCTGGGT 
CCGCCCGCGT CGCCAGCGCC AACGCCCACG CCTACAGCCA CTCCCACATC GCTGCAGCAT 
CAGAGCTCCG AGGAGAGTCC AAGTCAGAGC CAGAGCCAGA GTCCCAGTAG CAAGTCGAGT 
TTGAAGAAGG CACCATTCAA CTTCTTGCAC GCCTCAAATC CGAATTTGGT GGAATTCGAT 
TTCCATACCT CGAAAACACT ACTCCCCAAG ATGAGTGTGG GTAATACACT CGACCATGGC 
CATAATATAC AGGGCTTTGT GACGCTTAAG GATCTGATGC TGCGCAAACA GGAGGAGGAG 
GCCCAGGAAA TGTACAACAA TCGAGTGCAC TTGGGTGTGG AGAAGCACAA GCACGCACGG 
ACGGAATCCA CGGCCAGTCA GCAGAGCGCC ATGAGCAGCA GCGTGACAAA GCCGCTGGAA 
AAGTTGCCCA AGATCCAGAG CGTGAGTCTG CCCAAAACAC CCGACTACGA GATCAGTTTT 
AAGCCTGACG ACGAGAGTAT TAAGAGAACC AGAACTAAGG AAGGTCAGAA GTTGCGCGAC 
TTTGGTTACG AGCTGATATG TGGGGATGAG CCGAGCACTA GCTCCATCAG CCGGCAAGAG 
CACCACCATC ATCATCATCA TCACCAGCAC CACCTGCAGC ATGTGCAACA GCAGCAGCAG 
CTACAACATC AGCAGACGCA GCAGCAACAT AGCAGCAAGG CCAAGCACTT CTTGCGCTCA 
CAGCAGTTAC AGCTCTCCAG TTTCCTGCAC AAGAACAGCA GCGGCGGCAG CGGCAAGAGT 
TCCGGATCCG GTGCGGATCA AAAGAAGTCC AAAGGAAAGT CTAGCGGCGA TCGTCGGTTT 
CCCTTTTCCA AGCACTCAAC TGGCTCCGCG GGCAGTGGCT GTGGGCCGGG TCATGCGATG 
ACTATGACGC TGCCGCTGAA CAAGAAGTCC AAATCGAATC ACGCCTTGGA TGGAGCCGTA 
GGCAATGGAG TCACCATAAT CGGCAGCGGC AGCAGCATCA AGAAAAGCCA GACGTACAAT 
CAGGATCTGC GAGACAAGAT AGTGGGCACC AAGTACGATG CGCATCGCAA GAACTCGGCC 
CCGATACCAA TCTTTTCGAA ACTCTCCATA TCGGGTGGCA CGCCGGCGAA GCCCTCCAAG 
GAGAATCGTT TCCTGGGCTC ACCGCTGCTG CATCGCACGC TGTTCGGGCA CCATCACCAT 
CAGCAGCAGG CAGTAACGCC ACCAAGCAGT GCGGATCCCG ATTGCGATCA GGAGATCTTC 
TCCCAAATCA CCCTGCCCAC GCACAATCAG GCGGGATATC GAAGTTACCG TGGACCTGGC 
ATACTAACCA CCTCGACCAC TAATTTGTGC TCGTCGGAGG GCTTGCAGCC GCCGTTGCCC 
CCAGCGCCAC CGCCTCCCGT CAACACCAGC AGGCAGAGAG AGGAGGAGCA AGCGGAGGCA 
ACGATATCAG AGTGTGCGGC CACCCCAAAA GTGTCCAGTT CGGGATCTGT GGATTCGAAG 
GCGGCAACGC CGGACTATCC AAATATGGAG TGTCCGCCTG TCTTTGAGCC GGAAATCTAC 
TCGCTCAGCG ATACCAGCTT GTCCCGCATC GTGATGCGGA CGCCCAGCAG CAGTGGTGCC 
ACCAGTGCCA CCGCCAGCAC CACCACCACC AACAACAACA ACAACAATAA TAACACCAAT 
AACAACACCA ACACCAACAG CAGTCCCAGT GGCGGCGAAC ACCATCAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725671.1
CDS	121..4794
Translation	MAYINIAEWTPDQVTDWIKGLDESMKGYLYEFSKQEIGGRALLNIRPYELENLGMLRIGHQEIVLEAVENLRNFHYHLKNDNLQFMALHVATAAKNLHRELARNHAESTKIDTRILHDITRTIATLKPLVGSLERTPFRKQEMYREYCGNVLKCGLELATIAHRDRFALQPVPAIRQSAERLENLANFVIQDISDPMVLQPASLNLVTLKKRESELGFNIESSYNGIHRVTDIKYNSPAHNSGKIEDGDEIVQINYQTVVGWQHRTVLEHLREALPDVVLTVKKRPKHTKMFGQIYMQPYRLPSKKRNMAARWAAQMPSPRAAFLTLDTEQLATGGTGSAVPDPSKSLSSEKREVLTKANPVSSASDSDSSCSDIPTPTDPKLAAREIRLYYPKPRALLQRRNTICGCEYLSLKNSDLVVPSWHERKPGIGSPTNCDPGSPSIRDKSISFGYGLEMAARPTTCIGIAGDTSTDKARRMFHEARKLKQLTEDSQREFLVDRYKPGVSKVVRFDAKEDYVMKNEKFICNVENTILETFEPIPFADEGDEDALETLRNCKTENAEELLEAINLATKGQDLPLAEAINMPLLQQGRRGRLDKSHSTPAYDNSGEESDTPPAIEPRKEFLLVTPPAPPPRPRKQREMTPPAVPPPPPKPASMQPASSITSISIPVPVPVPAAVDPSEISELHTPSKSRTLTLKKKHSLMAKRRNTNLKLLGTGDIQGHLYRRKKNHRGVTYWARIYFVMLDTILYGFRSKQSTSASLVIFLPGFTVSLAKEVHSKPHAFKVYHTAKSFYFAAESLDALNQWVDFLRQASLKVPPSTGSKGGGDAKDLYSENDSSGEECDALVIQNLSTPSPQGNKESMSMSMTLSGGTPPSSAPIKHERGYLDSFRKFTNTFKSSAAKPSSDIPVPTEQYRSYRKVPGGSFGIQIGANTPGYHDPAMPPTQIPPLVGPKLSRSSSRSSVVSGTESAVSLSASILGPPASPAPTPTPTATPTSLQHQSSEESPSQSQSQSPSSKSSLKKAPFNFLHASNPNLVEFDFHTSKTLLPKMSVGNTLDHGHNIQGFVTLKDLMLRKQEEEAQEMYNNRVHLGVEKHKHARTESTASQQSAMSSSVTKPLEKLPKIQSVSLPKTPDYEISFKPDDESIKRTRTKEGQKLRDFGYELICGDEPSTSSISRQEHHHHHHHHQHHLQHVQQQQQLQHQQTQQQHSSKAKHFLRSQQLQLSSFLHKNSSGGSGKSSGSGADQKKSKGKSSGDRRFPFSKHSTGSAGSGCGPGHAMTMTLPLNKKSKSNHALDGAVGNGVTIIGSGSSIKKSQTYNQDLRDKIVGTKYDAHRKNSAPIPIFSKLSISGGTPAKPSKENRFLGSPLLHRTLFGHHHHQQQAVTPPSSADPDCDQEIFSQITLPTHNQAGYRSYRGPGILTTSTTNLCSSEGLQPPLPPAPPPPVNTSRQREEEQAEATISECAATPKVSSSGSVDSKAATPDYPNMECPPVFEPEIYSLSDTSLSRIVMRTPSSSGATSATASTTTTNNNNNNNNTNNNTNTNSSPSGGEHHQT

Target ORF information:

RefSeq Version	NM_166223.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster connector enhancer of ksr (cnk), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166223.2

ORF Insert Sequence:

ATGGCCTACA TCAACATCGC CGAGTGGACG CCCGACCAGG TCACCGACTG GATAAAAGGT 
CTGGATGAGT CTATGAAGGG GTATTTGTAC GAGTTCTCCA AGCAGGAGAT CGGCGGACGG 
GCGTTGCTCA ACATACGGCC ATACGAGCTG GAGAATCTGG GCATGCTGCG CATTGGCCAT 
CAGGAGATCG TGCTGGAGGC GGTGGAGAAC TTAAGAAACT TCCATTATCA CCTGAAAAAC 
GACAATCTGC AGTTCATGGC CTTGCATGTG GCCACGGCAG CTAAGAATCT GCATCGCGAA 
CTGGCCAGGA ATCATGCGGA GAGCACTAAG ATCGATACCC GGATACTGCA CGATATTACC 
AGGACGATAG CTACTCTAAA GCCATTGGTG GGCAGCCTGG AGCGAACACC GTTCCGTAAG 
CAGGAGATGT ACCGCGAGTA TTGTGGCAAT GTGCTCAAAT GCGGCCTGGA GCTGGCCACA 
ATTGCCCACC GTGATCGTTT TGCCCTGCAG CCGGTGCCAG CGATTCGACA GTCAGCGGAA 
CGGCTCGAGA ATCTTGCCAA CTTTGTAATC CAGGACATAT CTGATCCCAT GGTACTGCAG 
CCAGCCTCTC TGAACCTTGT CACCCTTAAA AAACGCGAAT CCGAGTTGGG TTTCAACATA 
GAGTCCAGTT ATAATGGCAT TCACCGTGTC ACGGATATCA AGTACAACTC GCCAGCTCAC 
AATTCCGGAA AAATCGAAGA TGGCGACGAG ATTGTGCAGA TCAACTACCA GACCGTTGTG 
GGCTGGCAGC ATCGCACAGT ACTGGAGCAT CTGAGGGAAG CACTTCCAGA TGTGGTGCTG 
ACGGTGAAGA AACGGCCGAA GCACACAAAG ATGTTTGGAC AGATCTATAT GCAGCCGTAC 
AGGTTGCCCA GCAAGAAGAG GAATATGGCC GCCCGCTGGG CGGCACAGAT GCCAAGTCCC 
CGTGCTGCAT TTTTAACCCT GGACACGGAG CAACTGGCAA CAGGAGGAAC AGGAAGCGCG 
GTGCCAGATC CCAGTAAATC CCTAAGCAGT GAGAAGAGGG AGGTACTTAC CAAGGCCAAT 
CCAGTGTCCT CTGCCTCCGA CTCTGATTCC AGTTGCAGTG ATATTCCAAC ACCAACGGAT 
CCCAAGCTAG CCGCACGGGA GATCCGTTTG TATTACCCAA AGCCAAGAGC TTTGCTCCAA 
CGAAGAAACA CTATTTGTGG ATGCGAGTAT CTTAGCCTGA AAAACTCGGA TCTAGTCGTT 
CCATCGTGGC ACGAAAGAAA GCCAGGAATT GGTTCGCCCA CCAATTGCGA CCCTGGCTCG 
CCCAGTATAC GGGATAAGTC GATATCCTTC GGTTATGGCC TAGAGATGGC AGCAAGGCCG 
ACCACTTGCA TTGGGATTGC GGGCGATACC TCCACGGACA AGGCCAGGCG AATGTTTCAT 
GAGGCGCGAA AACTAAAGCA GCTGACGGAG GACTCGCAGC GCGAATTCCT CGTGGATCGT 
TACAAGCCGG GTGTCAGCAA AGTAGTTCGG TTCGACGCCA AGGAAGATTA TGTGATGAAG 
AATGAAAAAT TTATCTGTAA CGTTGAGAAC ACCATACTGG AGACCTTTGA ACCGATTCCC 
TTTGCGGATG AGGGAGACGA GGACGCCTTG GAAACGCTGC GCAACTGCAA AACAGAAAAC 
GCAGAGGAGC TCCTAGAGGC AATTAATCTG GCTACAAAAG GTCAGGACTT GCCCCTGGCC 
GAGGCCATTA ACATGCCTTT GTTGCAGCAA GGTCGAAGGG GCCGATTAGA CAAGAGTCAT 
AGTACTCCGG CATACGATAA TTCGGGTGAA GAATCGGATA CGCCGCCAGC GATTGAGCCA 
AGAAAGGAGT TCCTGCTTGT CACACCGCCA GCACCGCCAC CGCGACCCCG CAAGCAAAGG 
GAAATGACGC CACCGGCGGT GCCTCCACCT CCACCAAAAC CAGCCAGCAT GCAGCCGGCT 
AGTAGTATCA CCAGTATCTC CATTCCCGTG CCAGTACCTG TACCAGCCGC AGTTGATCCA 
TCGGAGATCA GTGAGCTTCA TACGCCCAGC AAATCCCGGA CACTGACCCT TAAAAAGAAG 
CACAGCTTAA TGGCCAAGCG GCGGAACACA AATCTCAAGC TGTTGGGGAC TGGGGATATC 
CAAGGTCATC TGTACAGGCG CAAGAAAAAT CATCGCGGAG TGACTTACTG GGCGAGAATC 
TATTTCGTGA TGCTGGATAC GATACTGTAC GGATTTCGCA GTAAGCAGAG TACCAGCGCC 
AGCTTGGTAA TATTTTTACC GGGCTTTACG GTTTCATTGG CAAAAGAAGT GCACTCCAAA 
CCGCATGCCT TTAAAGTTTA CCACACGGCC AAGAGCTTCT ACTTTGCGGC CGAGTCGCTG 
GACGCACTTA ACCAATGGGT GGACTTCTTG CGCCAGGCTT CGCTCAAAGT GCCGCCTAGT 
ACGGGTTCAA AAGGCGGTGG TGACGCAAAG GATTTGTATT CGGAGAACGA TAGCTCAGGC 
GAGGAATGCG ATGCCCTTGT GATCCAGAAT TTAAGCACGC CCTCGCCGCA AGGCAACAAG 
GAGTCAATGT CCATGTCGAT GACACTATCT GGCGGAACAC CACCTTCCTC TGCACCCATT 
AAGCATGAAC GCGGCTACTT GGACTCGTTT CGCAAATTTA CGAATACATT CAAGAGCAGT 
GCAGCGAAAC CGTCGAGCGA CATCCCTGTG CCAACGGAGC AATACCGCAG CTATAGGAAG 
GTGCCAGGCG GAAGTTTTGG CATTCAGATT GGTGCCAATA CACCGGGCTA CCATGATCCC 
GCAATGCCGC CGACACAGAT TCCACCTTTG GTGGGTCCTA AACTGTCGCG TAGCTCAAGT 
AGGAGTTCGG TGGTCAGTGG AACCGAGTCT GCCGTATCAT TATCTGCTTC GATTCTGGGT 
CCGCCCGCGT CGCCAGCGCC AACGCCCACG CCTACAGCCA CTCCCACATC GCTGCAGCAT 
CAGAGCTCCG AGGAGAGTCC AAGTCAGAGC CAGAGCCAGA GTCCCAGTAG CAAGTCGAGT 
TTGAAGAAGG CACCATTCAA CTTCTTGCAC GCCTCAAATC CGAATTTGGT GGAATTCGAT 
TTCCATACCT CGAAAACACT ACTCCCCAAG ATGAGTGTGG GTAATACACT CGACCATGGC 
CATAATATAC AGGGCTTTGT GACGCTTAAG GATCTGATGC TGCGCAAACA GGAGGAGGAG 
GCCCAGGAAA TGTACAACAA TCGAGTGCAC TTGGGTGTGG AGAAGCACAA GCACGCACGG 
ACGGAATCCA CGGCCAGTCA GCAGAGCGCC ATGAGCAGCA GCGTGACAAA GCCGCTGGAA 
AAGTTGCCCA AGATCCAGAG CGTGAGTCTG CCCAAAACAC CCGACTACGA GATCAGTTTT 
AAGCCTGACG ACGAGAGTAT TAAGAGAACC AGAACTAAGG AAGGTCAGAA GTTGCGCGAC 
TTTGGTTACG AGCTGATATG TGGGGATGAG CCGAGCACTA GCTCCATCAG CCGGCAAGAG 
CACCACCATC ATCATCATCA TCACCAGCAC CACCTGCAGC ATGTGCAACA GCAGCAGCAG 
CTACAACATC AGCAGACGCA GCAGCAACAT AGCAGCAAGG CCAAGCACTT CTTGCGCTCA 
CAGCAGTTAC AGCTCTCCAG TTTCCTGCAC AAGAACAGCA GCGGCGGCAG CGGCAAGAGT 
TCCGGATCCG GTGCGGATCA AAAGAAGTCC AAAGGAAAGT CTAGCGGCGA TCGTCGGTTT 
CCCTTTTCCA AGCACTCAAC TGGCTCCGCG GGCAGTGGCT GTGGGCCGGG TCATGCGATG 
ACTATGACGC TGCCGCTGAA CAAGAAGTCC AAATCGAATC ACGCCTTGGA TGGAGCCGTA 
GGCAATGGAG TCACCATAAT CGGCAGCGGC AGCAGCATCA AGAAAAGCCA GACGTACAAT 
CAGGATCTGC GAGACAAGAT AGTGGGCACC AAGTACGATG CGCATCGCAA GAACTCGGCC 
CCGATACCAA TCTTTTCGAA ACTCTCCATA TCGGGTGGCA CGCCGGCGAA GCCCTCCAAG 
GAGAATCGTT TCCTGGGCTC ACCGCTGCTG CATCGCACGC TGTTCGGGCA CCATCACCAT 
CAGCAGCAGG CAGTAACGCC ACCAAGCAGT GCGGATCCCG ATTGCGATCA GGAGATCTTC 
TCCCAAATCA CCCTGCCCAC GCACAATCAG GCGGGATATC GAAGTTACCG TGGACCTGGC 
ATACTAACCA CCTCGACCAC TAATTTGTGC TCGTCGGAGG GCTTGCAGCC GCCGTTGCCC 
CCAGCGCCAC CGCCTCCCGT CAACACCAGC AGGCAGAGAG AGGAGGAGCA AGCGGAGGCA 
ACGATATCAG AGTGTGCGGC CACCCCAAAA GTGTCCAGTT CGGGATCTGT GGATTCGAAG 
GCGGCAACGC CGGACTATCC AAATATGGAG TGTCCGCCTG TCTTTGAGCC GGAAATCTAC 
TCGCTCAGCG ATACCAGCTT GTCCCGCATC GTGATGCGGA CGCCCAGCAG CAGTGGTGCC 
ACCAGTGCCA CCGCCAGCAC CACCACCACC AACAACAACA ACAACAATAA TAACACCAAT 
AACAACACCA ACACCAACAG CAGTCCCAGT GGCGGCGAAC ACCATCAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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cnk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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