fat-spondin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fat-spondin
Entrez Gene ID	36919
Full Name	CG6953 gene product from transcript CG6953-RA
Synonyms	CG6953,CT21501,Dmel\CG6953,Fat-spondin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53F2-53F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_058087.5	NP_477435.1	fat-spondin

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011765	XP_320745.4
Caenorhabditis elegans (roundworm)	spon-1	NP_495473.2
Drosophila melanogaster (fruit fly)	fat-spondin	NP_477435.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fat-spondin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fat-spondin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10310	NM_058087.5 Latest version!	Drosophila melanogaster fat-spondin (fat-spondin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fat-spondin ( NM_058087.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477435.1 Drosophila melanogaster fat-spondin (fat-spondin), mRNA.

Price & Availability↑

CloneID	OFa10310
Clone ID Related Accession (Same CDS sequence)	NM_058087.5
Accession Version	NM_058087.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fat-spondin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_058087.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGCAAT CGCTGCCGCT GCTCATCCGC CTCATCGTTG TGATCTGGCT GACAATTAGT 
ATGGTCACCG CATGTCCCCG GGCGCCCACC CACTTGAACC ACGGTCGACG CACACGCGGC 
GACAATGGAT ACAAATTAAT TGTGGCCGAT GGACCCAACG GCTATGTGCC CGGCAAGACG 
TATAATTTAC TTCTACTTGG ATCCCGGACT CACTTGAAAG TTCAGCATTT CACCCACTTT 
ACCATTACTG CGGAGGCACA CACTGGCGCC CGTCGTCCAC AGGCAGCTAG TCCCCGGCGA 
GTGGGCCGCT TCCAGCTGTT TAGTGACTCC CTGACCCAGT TCAACGACAG ATGCGTGAAC 
ACGGTTTCCG AGGCGGACGA CCTGCCCAAA ACAGAAGTCC AGGTAATGTG GGTGGCTCCG 
GAGTCGGGTT CTGGCTGTGT CTCTTTGTCA GCAATGGTCT ACGAGGGTCC GAGAGCCTGG 
TTCGCCGACG ATGGCAACCT ATCCACTGTG ATTTGCGAAC GAAAGCCTGA TGCAGCTGCT 
GCTCAAAAGG AATGCTGCGC CTGCGATGAA GCCAAATACA GCTTCGTTTT CGAGGGAATC 
TGGTCAAACG AAACCCATCC CAAGGATTAT CCGTTCGCCA TCTGGTTAAC ACACTTCTCT 
GACGTGATTG GTGCTTCGCA CGAGTCCAAC TTCTCCTTCT GGGGCGAGAA CCACATAGCC 
ACCGCCGGAT TCCGCTCTCT GGCGGAGTGG GGTTCTCCGG CTGCTCTAGA AACGGAGCTG 
CGCGCGAACG GGCCCAAGTT GCGCACCCTG ATTAAGGCCG CTGGGTTGTG GTACCCGAAT 
GTCAACCAGA ACACCTCATC CAAGTTCCGG GTGGATCGCA AGCACCCTAA GGTATCGCTG 
GTGTCCATGT TTGGTCCTTC TCCCGATTGG GTGGTGGGCA TTAGTGGTCT GGATCTCTGC 
ACTGAGGATT GCAGCTGGAA GGAGTCAATG GACTTTGATT TGTTTCCATG GGATGCGGGC 
ACTGATAGCG GCATCTCTTA TATGTCACCC AACTCGGAGA CACAACCTCC GGAAAGGATG 
TATCGCATCA CTACAATGTA TCCTGAGGAT CCTAGGGCTC CCTTTTATAA TCCCAAGAGC 
CGTGAGATGA CACCACTTGC CAAGCTATAC CTAAGACGTG AGAAGATTGT CTCCCGGAAC 
TGCGACGATG AGTTCCTGCA GGCTCTTCAG TTGGAGGTGT CCGACGATGC TGAGGAGCAG 
GACACCAGAG CCGAATGTCG AGTGGGCGAC TACTCCGCCT GGAGTCCGTG TTCCGTGAGC 
TGCGGCAAGG GAATCCGCAT GCGCAGCAGG CAATATCTGT ATCCCGCGGC GGCGGATCAG 
AACAAATGCG CTCGCCAACT GGTGGCCAAG GAAATGTGCG TGGCTGCCAT ACCCGAATGT 
GCCGATGGCC CAGCCCAGTC CAAGGATCGT GACGATGATG AGGGCGAGAA CCTGGCCAAC 
TCGCAGTCCC TGGTGGGCAG CAATGGCGAG GGAGCCGGTC TCTGCAAAAC TTCTCCCTGG 
AGCGTGTGGT CCGAGTGTTC GGCCAGCTGT GGCATTGGCA TTACCATGCG CACGCGCACC 
TTTGTCAATC ATTTGGGACG CAAGCGATGT CCCCATATCA CCATAGTGGA AAAGAACAAG 
TGCATGCGGC CGGACTGTAC ATACGAGCAG GTGGAGCTTC CTGATCCGCA ATGTCCCACC 
AGCCAGTGGA GCGACTGGAG TCCGTGCTCC TCGACCTGCG GCCGGGGAGT TACCATTCGG 
ACTCGGCTGC TTCTGTTGGA GAATGGACCC GATAAGGAGA GCTGTACGCA ACGCATGGAG 
CTGCATCAGC AAAAGGAGTG CGTCAATCCC ATTGACTGCC ACATTAACGC GGAGCAGGCC 
AAGGACATCT GTGTACAGGC ACCGGACCCA GGTCCCTGTC GTGGCACCTA TATGCGGTAT 
GCCTACGATC CGCAGAACCA GCATTGCTAC TCCTTTACCT ACGGAGGATG CCGTGGCAAC 
CGAAACAACT TCCTCACGGA GAACGACTGC CTGAATACGT GCAATGTGCT TAGGTCGCCG 
TATTCCTCAA GAGTGGATCA GCCCAGGGCC TGCGTCCTGT CAGATTGGTC CGTCTGGTCG 
CCCTGCAGCG TTAGCTGTGG TGTCGGAGTC TCTGAAAGCC GGCGCTACGT TGTCACCGAG 
CCCCAGAATG GTGGACAGCC GTGCTCCAAG CGCCTGGTCA AATCACGCTC TTGCGCCATG 
CCGGCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477435.1
CDS	98..2389
Translation	MGQSLPLLIRLIVVIWLTISMVTACPRAPTHLNHGRRTRGDNGYKLIVADGPNGYVPGKTYNLLLLGSRTHLKVQHFTHFTITAEAHTGARRPQAASPRRVGRFQLFSDSLTQFNDRCVNTVSEADDLPKTEVQVMWVAPESGSGCVSLSAMVYEGPRAWFADDGNLSTVICERKPDAAAAQKECCACDEAKYSFVFEGIWSNETHPKDYPFAIWLTHFSDVIGASHESNFSFWGENHIATAGFRSLAEWGSPAALETELRANGPKLRTLIKAAGLWYPNVNQNTSSKFRVDRKHPKVSLVSMFGPSPDWVVGISGLDLCTEDCSWKESMDFDLFPWDAGTDSGISYMSPNSETQPPERMYRITTMYPEDPRAPFYNPKSREMTPLAKLYLRREKIVSRNCDDEFLQALQLEVSDDAEEQDTRAECRVGDYSAWSPCSVSCGKGIRMRSRQYLYPAAADQNKCARQLVAKEMCVAAIPECADGPAQSKDRDDDEGENLANSQSLVGSNGEGAGLCKTSPWSVWSECSASCGIGITMRTRTFVNHLGRKRCPHITIVEKNKCMRPDCTYEQVELPDPQCPTSQWSDWSPCSSTCGRGVTIRTRLLLLENGPDKESCTQRMELHQQKECVNPIDCHINAEQAKDICVQAPDPGPCRGTYMRYAYDPQNQHCYSFTYGGCRGNRNNFLTENDCLNTCNVLRSPYSSRVDQPRACVLSDWSVWSPCSVSCGVGVSESRRYVVTEPQNGGQPCSKRLVKSRSCAMPAC

Target ORF information:

RefSeq Version	NM_058087.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fat-spondin (fat-spondin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058087.5

ORF Insert Sequence:

ATGGGGCAAT CGCTGCCGCT GCTCATCCGC CTCATCGTTG TGATCTGGCT GACAATTAGT 
ATGGTCACCG CATGTCCCCG GGCGCCCACC CACTTGAACC ACGGTCGACG CACACGCGGC 
GACAATGGAT ACAAATTAAT TGTGGCCGAT GGACCCAACG GCTATGTGCC CGGCAAGACG 
TATAATTTAC TTCTACTTGG ATCCCGGACT CACTTGAAAG TTCAGCATTT CACCCACTTT 
ACCATTACTG CGGAGGCACA CACTGGCGCC CGTCGTCCAC AGGCAGCTAG TCCCCGGCGA 
GTGGGCCGCT TCCAGCTGTT TAGTGACTCC CTGACCCAGT TCAACGACAG ATGCGTGAAC 
ACGGTTTCCG AGGCGGACGA CCTGCCCAAA ACAGAAGTCC AGGTAATGTG GGTGGCTCCG 
GAGTCGGGTT CTGGCTGTGT CTCTTTGTCA GCAATGGTCT ACGAGGGTCC GAGAGCCTGG 
TTCGCCGACG ATGGCAACCT ATCCACTGTG ATTTGCGAAC GAAAGCCTGA TGCAGCTGCT 
GCTCAAAAGG AATGCTGCGC CTGCGATGAA GCCAAATACA GCTTCGTTTT CGAGGGAATC 
TGGTCAAACG AAACCCATCC CAAGGATTAT CCGTTCGCCA TCTGGTTAAC ACACTTCTCT 
GACGTGATTG GTGCTTCGCA CGAGTCCAAC TTCTCCTTCT GGGGCGAGAA CCACATAGCC 
ACCGCCGGAT TCCGCTCTCT GGCGGAGTGG GGTTCTCCGG CTGCTCTAGA AACGGAGCTG 
CGCGCGAACG GGCCCAAGTT GCGCACCCTG ATTAAGGCCG CTGGGTTGTG GTACCCGAAT 
GTCAACCAGA ACACCTCATC CAAGTTCCGG GTGGATCGCA AGCACCCTAA GGTATCGCTG 
GTGTCCATGT TTGGTCCTTC TCCCGATTGG GTGGTGGGCA TTAGTGGTCT GGATCTCTGC 
ACTGAGGATT GCAGCTGGAA GGAGTCAATG GACTTTGATT TGTTTCCATG GGATGCGGGC 
ACTGATAGCG GCATCTCTTA TATGTCACCC AACTCGGAGA CACAACCTCC GGAAAGGATG 
TATCGCATCA CTACAATGTA TCCTGAGGAT CCTAGGGCTC CCTTTTATAA TCCCAAGAGC 
CGTGAGATGA CACCACTTGC CAAGCTATAC CTAAGACGTG AGAAGATTGT CTCCCGGAAC 
TGCGACGATG AGTTCCTGCA GGCTCTTCAG TTGGAGGTGT CCGACGATGC TGAGGAGCAG 
GACACCAGAG CCGAATGTCG AGTGGGCGAC TACTCCGCCT GGAGTCCGTG TTCCGTGAGC 
TGCGGCAAGG GAATCCGCAT GCGCAGCAGG CAATATCTGT ATCCCGCGGC GGCGGATCAG 
AACAAATGCG CTCGCCAACT GGTGGCCAAG GAAATGTGCG TGGCTGCCAT ACCCGAATGT 
GCCGATGGCC CAGCCCAGTC CAAGGATCGT GACGATGATG AGGGCGAGAA CCTGGCCAAC 
TCGCAGTCCC TGGTGGGCAG CAATGGCGAG GGAGCCGGTC TCTGCAAAAC TTCTCCCTGG 
AGCGTGTGGT CCGAGTGTTC GGCCAGCTGT GGCATTGGCA TTACCATGCG CACGCGCACC 
TTTGTCAATC ATTTGGGACG CAAGCGATGT CCCCATATCA CCATAGTGGA AAAGAACAAG 
TGCATGCGGC CGGACTGTAC ATACGAGCAG GTGGAGCTTC CTGATCCGCA ATGTCCCACC 
AGCCAGTGGA GCGACTGGAG TCCGTGCTCC TCGACCTGCG GCCGGGGAGT TACCATTCGG 
ACTCGGCTGC TTCTGTTGGA GAATGGACCC GATAAGGAGA GCTGTACGCA ACGCATGGAG 
CTGCATCAGC AAAAGGAGTG CGTCAATCCC ATTGACTGCC ACATTAACGC GGAGCAGGCC 
AAGGACATCT GTGTACAGGC ACCGGACCCA GGTCCCTGTC GTGGCACCTA TATGCGGTAT 
GCCTACGATC CGCAGAACCA GCATTGCTAC TCCTTTACCT ACGGAGGATG CCGTGGCAAC 
CGAAACAACT TCCTCACGGA GAACGACTGC CTGAATACGT GCAATGTGCT TAGGTCGCCG 
TATTCCTCAA GAGTGGATCA GCCCAGGGCC TGCGTCCTGT CAGATTGGTC CGTCTGGTCG 
CCCTGCAGCG TTAGCTGTGG TGTCGGAGTC TCTGAAAGCC GGCGCTACGT TGTCACCGAG 
CCCCAGAATG GTGGACAGCC GTGCTCCAAG CGCCTGGTCA AATCACGCTC TTGCGCCATG 
CCGGCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fat-spondin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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