CG5522 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5522
Entrez Gene ID	36881
Full Name	CG5522 gene product from transcript CG5522-RE
Synonyms	Dmel\CG5522,RalGPS,RasGPS
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53D8-53D9

mRNA and Protein(s)

mRNA	Protein	Name
NM_166193.2	NP_725613.1	uncharacterized protein, isoform A
NM_137314.3	NP_611158.1	uncharacterized protein, isoform C
NM_166197.2	NP_725617.1	uncharacterized protein, isoform F
NM_166196.2	NP_725616.1	uncharacterized protein, isoform D
NM_166194.2	NP_725614.1	uncharacterized protein, isoform B
NM_166195.2	NP_725615.1	uncharacterized protein, isoform E

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004853	XP_314338.4
Mus musculus (house mouse)	Ralgps1	NP_780420.1
Danio rerio (zebrafish)	ralgps1	NP_001093616.1
Drosophila melanogaster (fruit fly)	CG5522	NP_611158.1
Bos taurus (cattle)	RALGPS1	NP_001180029.1
Rattus norvegicus (Norway rat)	Ralgps1	XP_006234079.1
Xenopus tropicalis (tropical clawed frog)	ralgps1	XP_002942011.2
Homo sapiens (human)	RALGPS1	NP_055451.1
Pan troglodytes (chimpanzee)	RALGPS1	XP_003951576.1
Canis lupus familiaris (dog)	RALGPS1	XP_005625400.1
Gallus gallus (chicken)	RALGPS1	XP_003642349.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5522 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5522 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10273	NM_166197.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant F (CG5522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10273	NM_137314.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG5522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10273	NM_166196.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG5522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10272	NM_166195.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant E (CG5522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10272	NM_166194.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10272	NM_166193.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5522), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5522 ( NM_137314.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611158.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG5522), mRNA.

Price & Availability↑

CloneID	OFa10273
Clone ID Related Accession (Same CDS sequence)	NM_137314.3 , NM_166197.2 , NM_166196.2
Accession Version	NM_137314.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137314.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAACATTT TTGGCAAGAT TGCAACGCAC AACCACAGCG ATGGACATCC TCACCACCTG 
CACCTGGAAC TTGATCCGAG TCAAGTGCAG CCTCGTCACC ACCTGCTAGA TGATTCGGTC 
TTGGAGCACA GCGATGCCCT ATCCCAAGCA GATCTAACAT CGTTGGAAAG TGCAGAGGGA 
ATTATGTGTG ATTTTACCGG CAGCGATTGC GATTTGATGT CACCAGAGGC AGCAGCCGCC 
ATGCACGGAT GTGTCCGGCG TAAAACAGTT CAAAAGGAGG GAAGGAAGCC AGCGGTGGCA 
TCTTGGCAAC GATACTGGCT ACAGATTTGG GCCAACTCGC TGGTGTATTT CCCACCCAAA 
TCCTTTAAAG GAAGCGAACG CAACGACTTT AAGCGGGAAC CGTGCAAAGT GTGTCCATTG 
GATGGATGGT ATGCCCACGT GAGCGATAAC ACCAAGCACA AGAACACCTT CGAACTGTGC 
CACCGAACTC TGGGCACCGT CTATCGCTTC CGGACGGACA GTCCCCAGAT GACGCATCTC 
TGGTCGAATG CCATTTGCAA ATTGGCCACC ATGCGAGTGC CCAAACCTCT GCCCACCAAC 
CTAATGTCCT TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611158.1
CDS	398..2395
Translation	MMRYSEISRDLSTDNLRYSELSRKPTTDYNGYTGQAYAATQFKQSSPPPPPSQQQQQPTRVSKQRSPNTNGSAHTMACLRQEKTYKEQQMEPPSMQPQASKTLNIKSTRRKNSIGCLNSFSSSPDSPGCYYTIAASAAPPSKSQSLPAHASIKQLDAVILSALRVPADVLANQITLLDFPVFAQIQPDELSSCAWTKKDKHVNTPNIVAFTKRFNHTSFWTVQEILNAEQPKQRAEIITHFIKVAKKLHELNNLHSLFAIISAMQSASIYRLTKTWACLSKKDRNAFDRLSDIFSDQDNWANLRSYLESLRLPCIPYLGLFLTDLIYIDLAHPHKGGLEPEQRRNKMNNILRVISNYQQSNYKHLQKHEATQKYLTSIRYIEELQNIFEEDQYKRSLNLEPASPSGPSSSSCSSKESFNVEVVTPALGCLNLSPAKTIGSMRMASGTKFVPGHRKCRSLGTNIFGKIATHNHSDGHPHHLHLELDPSQVQPRHHLLDDSVLEHSDALSQADLTSLESAEGIMCDFTGSDCDLMSPEAAAAMHGCVRRKTVQKEGRKPAVASWQRYWLQIWANSLVYFPPKSFKGSERNDFKREPCKVCPLDGWYAHVSDNTKHKNTFELCHRTLGTVYRFRTDSPQMTHLWSNAICKLATMRVPKPLPTNLMSFE

Target ORF information:

RefSeq Version	NM_137314.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG5522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137314.3

ORF Insert Sequence:

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAACATTT TTGGCAAGAT TGCAACGCAC AACCACAGCG ATGGACATCC TCACCACCTG 
CACCTGGAAC TTGATCCGAG TCAAGTGCAG CCTCGTCACC ACCTGCTAGA TGATTCGGTC 
TTGGAGCACA GCGATGCCCT ATCCCAAGCA GATCTAACAT CGTTGGAAAG TGCAGAGGGA 
ATTATGTGTG ATTTTACCGG CAGCGATTGC GATTTGATGT CACCAGAGGC AGCAGCCGCC 
ATGCACGGAT GTGTCCGGCG TAAAACAGTT CAAAAGGAGG GAAGGAAGCC AGCGGTGGCA 
TCTTGGCAAC GATACTGGCT ACAGATTTGG GCCAACTCGC TGGTGTATTT CCCACCCAAA 
TCCTTTAAAG GAAGCGAACG CAACGACTTT AAGCGGGAAC CGTGCAAAGT GTGTCCATTG 
GATGGATGGT ATGCCCACGT GAGCGATAAC ACCAAGCACA AGAACACCTT CGAACTGTGC 
CACCGAACTC TGGGCACCGT CTATCGCTTC CGGACGGACA GTCCCCAGAT GACGCATCTC 
TGGTCGAATG CCATTTGCAA ATTGGCCACC ATGCGAGTGC CCAAACCTCT GCCCACCAAC 
CTAATGTCCT TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5522 ( NM_166197.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725617.1 Drosophila melanogaster uncharacterized protein, transcript variant F (CG5522), mRNA.

Price & Availability↑

CloneID	OFa10273
Clone ID Related Accession (Same CDS sequence)	NM_137314.3 , NM_166197.2 , NM_166196.2
Accession Version	NM_166197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166197.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAACATTT TTGGCAAGAT TGCAACGCAC AACCACAGCG ATGGACATCC TCACCACCTG 
CACCTGGAAC TTGATCCGAG TCAAGTGCAG CCTCGTCACC ACCTGCTAGA TGATTCGGTC 
TTGGAGCACA GCGATGCCCT ATCCCAAGCA GATCTAACAT CGTTGGAAAG TGCAGAGGGA 
ATTATGTGTG ATTTTACCGG CAGCGATTGC GATTTGATGT CACCAGAGGC AGCAGCCGCC 
ATGCACGGAT GTGTCCGGCG TAAAACAGTT CAAAAGGAGG GAAGGAAGCC AGCGGTGGCA 
TCTTGGCAAC GATACTGGCT ACAGATTTGG GCCAACTCGC TGGTGTATTT CCCACCCAAA 
TCCTTTAAAG GAAGCGAACG CAACGACTTT AAGCGGGAAC CGTGCAAAGT GTGTCCATTG 
GATGGATGGT ATGCCCACGT GAGCGATAAC ACCAAGCACA AGAACACCTT CGAACTGTGC 
CACCGAACTC TGGGCACCGT CTATCGCTTC CGGACGGACA GTCCCCAGAT GACGCATCTC 
TGGTCGAATG CCATTTGCAA ATTGGCCACC ATGCGAGTGC CCAAACCTCT GCCCACCAAC 
CTAATGTCCT TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725617.1
CDS	360..2357
Translation	MMRYSEISRDLSTDNLRYSELSRKPTTDYNGYTGQAYAATQFKQSSPPPPPSQQQQQPTRVSKQRSPNTNGSAHTMACLRQEKTYKEQQMEPPSMQPQASKTLNIKSTRRKNSIGCLNSFSSSPDSPGCYYTIAASAAPPSKSQSLPAHASIKQLDAVILSALRVPADVLANQITLLDFPVFAQIQPDELSSCAWTKKDKHVNTPNIVAFTKRFNHTSFWTVQEILNAEQPKQRAEIITHFIKVAKKLHELNNLHSLFAIISAMQSASIYRLTKTWACLSKKDRNAFDRLSDIFSDQDNWANLRSYLESLRLPCIPYLGLFLTDLIYIDLAHPHKGGLEPEQRRNKMNNILRVISNYQQSNYKHLQKHEATQKYLTSIRYIEELQNIFEEDQYKRSLNLEPASPSGPSSSSCSSKESFNVEVVTPALGCLNLSPAKTIGSMRMASGTKFVPGHRKCRSLGTNIFGKIATHNHSDGHPHHLHLELDPSQVQPRHHLLDDSVLEHSDALSQADLTSLESAEGIMCDFTGSDCDLMSPEAAAAMHGCVRRKTVQKEGRKPAVASWQRYWLQIWANSLVYFPPKSFKGSERNDFKREPCKVCPLDGWYAHVSDNTKHKNTFELCHRTLGTVYRFRTDSPQMTHLWSNAICKLATMRVPKPLPTNLMSFE

Target ORF information:

RefSeq Version	NM_166197.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant F (CG5522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166197.2

ORF Insert Sequence:

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAACATTT TTGGCAAGAT TGCAACGCAC AACCACAGCG ATGGACATCC TCACCACCTG 
CACCTGGAAC TTGATCCGAG TCAAGTGCAG CCTCGTCACC ACCTGCTAGA TGATTCGGTC 
TTGGAGCACA GCGATGCCCT ATCCCAAGCA GATCTAACAT CGTTGGAAAG TGCAGAGGGA 
ATTATGTGTG ATTTTACCGG CAGCGATTGC GATTTGATGT CACCAGAGGC AGCAGCCGCC 
ATGCACGGAT GTGTCCGGCG TAAAACAGTT CAAAAGGAGG GAAGGAAGCC AGCGGTGGCA 
TCTTGGCAAC GATACTGGCT ACAGATTTGG GCCAACTCGC TGGTGTATTT CCCACCCAAA 
TCCTTTAAAG GAAGCGAACG CAACGACTTT AAGCGGGAAC CGTGCAAAGT GTGTCCATTG 
GATGGATGGT ATGCCCACGT GAGCGATAAC ACCAAGCACA AGAACACCTT CGAACTGTGC 
CACCGAACTC TGGGCACCGT CTATCGCTTC CGGACGGACA GTCCCCAGAT GACGCATCTC 
TGGTCGAATG CCATTTGCAA ATTGGCCACC ATGCGAGTGC CCAAACCTCT GCCCACCAAC 
CTAATGTCCT TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5522 ( NM_166196.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725616.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG5522), mRNA.

Price & Availability↑

CloneID	OFa10273
Clone ID Related Accession (Same CDS sequence)	NM_137314.3 , NM_166197.2 , NM_166196.2
Accession Version	NM_166196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166196.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAACATTT TTGGCAAGAT TGCAACGCAC AACCACAGCG ATGGACATCC TCACCACCTG 
CACCTGGAAC TTGATCCGAG TCAAGTGCAG CCTCGTCACC ACCTGCTAGA TGATTCGGTC 
TTGGAGCACA GCGATGCCCT ATCCCAAGCA GATCTAACAT CGTTGGAAAG TGCAGAGGGA 
ATTATGTGTG ATTTTACCGG CAGCGATTGC GATTTGATGT CACCAGAGGC AGCAGCCGCC 
ATGCACGGAT GTGTCCGGCG TAAAACAGTT CAAAAGGAGG GAAGGAAGCC AGCGGTGGCA 
TCTTGGCAAC GATACTGGCT ACAGATTTGG GCCAACTCGC TGGTGTATTT CCCACCCAAA 
TCCTTTAAAG GAAGCGAACG CAACGACTTT AAGCGGGAAC CGTGCAAAGT GTGTCCATTG 
GATGGATGGT ATGCCCACGT GAGCGATAAC ACCAAGCACA AGAACACCTT CGAACTGTGC 
CACCGAACTC TGGGCACCGT CTATCGCTTC CGGACGGACA GTCCCCAGAT GACGCATCTC 
TGGTCGAATG CCATTTGCAA ATTGGCCACC ATGCGAGTGC CCAAACCTCT GCCCACCAAC 
CTAATGTCCT TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725616.1
CDS	259..2256
Translation	MMRYSEISRDLSTDNLRYSELSRKPTTDYNGYTGQAYAATQFKQSSPPPPPSQQQQQPTRVSKQRSPNTNGSAHTMACLRQEKTYKEQQMEPPSMQPQASKTLNIKSTRRKNSIGCLNSFSSSPDSPGCYYTIAASAAPPSKSQSLPAHASIKQLDAVILSALRVPADVLANQITLLDFPVFAQIQPDELSSCAWTKKDKHVNTPNIVAFTKRFNHTSFWTVQEILNAEQPKQRAEIITHFIKVAKKLHELNNLHSLFAIISAMQSASIYRLTKTWACLSKKDRNAFDRLSDIFSDQDNWANLRSYLESLRLPCIPYLGLFLTDLIYIDLAHPHKGGLEPEQRRNKMNNILRVISNYQQSNYKHLQKHEATQKYLTSIRYIEELQNIFEEDQYKRSLNLEPASPSGPSSSSCSSKESFNVEVVTPALGCLNLSPAKTIGSMRMASGTKFVPGHRKCRSLGTNIFGKIATHNHSDGHPHHLHLELDPSQVQPRHHLLDDSVLEHSDALSQADLTSLESAEGIMCDFTGSDCDLMSPEAAAAMHGCVRRKTVQKEGRKPAVASWQRYWLQIWANSLVYFPPKSFKGSERNDFKREPCKVCPLDGWYAHVSDNTKHKNTFELCHRTLGTVYRFRTDSPQMTHLWSNAICKLATMRVPKPLPTNLMSFE

Target ORF information:

RefSeq Version	NM_166196.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG5522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166196.2

ORF Insert Sequence:

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAACATTT TTGGCAAGAT TGCAACGCAC AACCACAGCG ATGGACATCC TCACCACCTG 
CACCTGGAAC TTGATCCGAG TCAAGTGCAG CCTCGTCACC ACCTGCTAGA TGATTCGGTC 
TTGGAGCACA GCGATGCCCT ATCCCAAGCA GATCTAACAT CGTTGGAAAG TGCAGAGGGA 
ATTATGTGTG ATTTTACCGG CAGCGATTGC GATTTGATGT CACCAGAGGC AGCAGCCGCC 
ATGCACGGAT GTGTCCGGCG TAAAACAGTT CAAAAGGAGG GAAGGAAGCC AGCGGTGGCA 
TCTTGGCAAC GATACTGGCT ACAGATTTGG GCCAACTCGC TGGTGTATTT CCCACCCAAA 
TCCTTTAAAG GAAGCGAACG CAACGACTTT AAGCGGGAAC CGTGCAAAGT GTGTCCATTG 
GATGGATGGT ATGCCCACGT GAGCGATAAC ACCAAGCACA AGAACACCTT CGAACTGTGC 
CACCGAACTC TGGGCACCGT CTATCGCTTC CGGACGGACA GTCCCCAGAT GACGCATCTC 
TGGTCGAATG CCATTTGCAA ATTGGCCACC ATGCGAGTGC CCAAACCTCT GCCCACCAAC 
CTAATGTCCT TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5522 ( NM_166193.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725613.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5522), mRNA.

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CloneID	OFa10272
Clone ID Related Accession (Same CDS sequence)	NM_166193.2 , NM_166194.2 , NM_166195.2
Accession Version	NM_166193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166193.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAAATTTC GCAGCAGCAG CCTGCCGCGA AACTTTGCCG AGAAGTGCCA ATGTTGCATC 
GTCATGATTG CGCCGGGCAT CGGCACCATC TCCAACAAGC GTTGCAGATG CCGTCGCATT 
TTTGGCAAGA TTGCAACGCA CAACCACAGC GATGGACATC CTCACCACCT GCACCTGGAA 
CTTGATCCGA GTCAAGTGCA GCCTCGTCAC CACCTGCTAG ATGATTCGGT CTTGGAGCAC 
AGCGATGCCC TATCCCAAGC AGATCTAACA TCGTTGGAAA GTGCAGAGGG AATTATGTGT 
GATTTTACCG GCAGCGATTG CGATTTGATG TCACCAGAGG CAGCAGCCGC CATGCACGGA 
TGTGTCCGGC GTAAAACAGT TCAAAAGGAG GGAAGGAAGC CAGCGGTGGC ATCTTGGCAA 
CGATACTGGC TACAGATTTG GGCCAACTCG CTGGTGTATT TCCCACCCAA ATCCTTTAAA 
GGAAGCGAAC GCAACGACTT TAAGCGGGAA CCGTGCAAAG TGTGTCCATT GGATGGATGG 
TATGCCCACG TGAGCGATAA CACCAAGCAC AAGAACACCT TCGAACTGTG CCACCGAACT 
CTGGGCACCG TCTATCGCTT CCGGACGGAC AGTCCCCAGA TGACGCATCT CTGGTCGAAT 
GCCATTTGCA AATTGGCCAC CATGCGAGTG CCCAAACCTC TGCCCACCAA CCTAATGTCC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725613.1
CDS	259..2367
Translation	MMRYSEISRDLSTDNLRYSELSRKPTTDYNGYTGQAYAATQFKQSSPPPPPSQQQQQPTRVSKQRSPNTNGSAHTMACLRQEKTYKEQQMEPPSMQPQASKTLNIKSTRRKNSIGCLNSFSSSPDSPGCYYTIAASAAPPSKSQSLPAHASIKQLDAVILSALRVPADVLANQITLLDFPVFAQIQPDELSSCAWTKKDKHVNTPNIVAFTKRFNHTSFWTVQEILNAEQPKQRAEIITHFIKVAKKLHELNNLHSLFAIISAMQSASIYRLTKTWACLSKKDRNAFDRLSDIFSDQDNWANLRSYLESLRLPCIPYLGLFLTDLIYIDLAHPHKGGLEPEQRRNKMNNILRVISNYQQSNYKHLQKHEATQKYLTSIRYIEELQNIFEEDQYKRSLNLEPASPSGPSSSSCSSKESFNVEVVTPALGCLNLSPAKTIGSMRMASGTKFVPGHRKCRSLGTKFRSSSLPRNFAEKCQCCIVMIAPGIGTISNKRCRCRRIFGKIATHNHSDGHPHHLHLELDPSQVQPRHHLLDDSVLEHSDALSQADLTSLESAEGIMCDFTGSDCDLMSPEAAAAMHGCVRRKTVQKEGRKPAVASWQRYWLQIWANSLVYFPPKSFKGSERNDFKREPCKVCPLDGWYAHVSDNTKHKNTFELCHRTLGTVYRFRTDSPQMTHLWSNAICKLATMRVPKPLPTNLMSFE

Target ORF information:

RefSeq Version	NM_166193.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166193.2

ORF Insert Sequence:

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAAATTTC GCAGCAGCAG CCTGCCGCGA AACTTTGCCG AGAAGTGCCA ATGTTGCATC 
GTCATGATTG CGCCGGGCAT CGGCACCATC TCCAACAAGC GTTGCAGATG CCGTCGCATT 
TTTGGCAAGA TTGCAACGCA CAACCACAGC GATGGACATC CTCACCACCT GCACCTGGAA 
CTTGATCCGA GTCAAGTGCA GCCTCGTCAC CACCTGCTAG ATGATTCGGT CTTGGAGCAC 
AGCGATGCCC TATCCCAAGC AGATCTAACA TCGTTGGAAA GTGCAGAGGG AATTATGTGT 
GATTTTACCG GCAGCGATTG CGATTTGATG TCACCAGAGG CAGCAGCCGC CATGCACGGA 
TGTGTCCGGC GTAAAACAGT TCAAAAGGAG GGAAGGAAGC CAGCGGTGGC ATCTTGGCAA 
CGATACTGGC TACAGATTTG GGCCAACTCG CTGGTGTATT TCCCACCCAA ATCCTTTAAA 
GGAAGCGAAC GCAACGACTT TAAGCGGGAA CCGTGCAAAG TGTGTCCATT GGATGGATGG 
TATGCCCACG TGAGCGATAA CACCAAGCAC AAGAACACCT TCGAACTGTG CCACCGAACT 
CTGGGCACCG TCTATCGCTT CCGGACGGAC AGTCCCCAGA TGACGCATCT CTGGTCGAAT 
GCCATTTGCA AATTGGCCAC CATGCGAGTG CCCAAACCTC TGCCCACCAA CCTAATGTCC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5522 ( NM_166194.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725614.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5522), mRNA.

Price & Availability↑

CloneID	OFa10272
Clone ID Related Accession (Same CDS sequence)	NM_166193.2 , NM_166194.2 , NM_166195.2
Accession Version	NM_166194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166194.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAAATTTC GCAGCAGCAG CCTGCCGCGA AACTTTGCCG AGAAGTGCCA ATGTTGCATC 
GTCATGATTG CGCCGGGCAT CGGCACCATC TCCAACAAGC GTTGCAGATG CCGTCGCATT 
TTTGGCAAGA TTGCAACGCA CAACCACAGC GATGGACATC CTCACCACCT GCACCTGGAA 
CTTGATCCGA GTCAAGTGCA GCCTCGTCAC CACCTGCTAG ATGATTCGGT CTTGGAGCAC 
AGCGATGCCC TATCCCAAGC AGATCTAACA TCGTTGGAAA GTGCAGAGGG AATTATGTGT 
GATTTTACCG GCAGCGATTG CGATTTGATG TCACCAGAGG CAGCAGCCGC CATGCACGGA 
TGTGTCCGGC GTAAAACAGT TCAAAAGGAG GGAAGGAAGC CAGCGGTGGC ATCTTGGCAA 
CGATACTGGC TACAGATTTG GGCCAACTCG CTGGTGTATT TCCCACCCAA ATCCTTTAAA 
GGAAGCGAAC GCAACGACTT TAAGCGGGAA CCGTGCAAAG TGTGTCCATT GGATGGATGG 
TATGCCCACG TGAGCGATAA CACCAAGCAC AAGAACACCT TCGAACTGTG CCACCGAACT 
CTGGGCACCG TCTATCGCTT CCGGACGGAC AGTCCCCAGA TGACGCATCT CTGGTCGAAT 
GCCATTTGCA AATTGGCCAC CATGCGAGTG CCCAAACCTC TGCCCACCAA CCTAATGTCC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725614.1
CDS	360..2468
Translation	MMRYSEISRDLSTDNLRYSELSRKPTTDYNGYTGQAYAATQFKQSSPPPPPSQQQQQPTRVSKQRSPNTNGSAHTMACLRQEKTYKEQQMEPPSMQPQASKTLNIKSTRRKNSIGCLNSFSSSPDSPGCYYTIAASAAPPSKSQSLPAHASIKQLDAVILSALRVPADVLANQITLLDFPVFAQIQPDELSSCAWTKKDKHVNTPNIVAFTKRFNHTSFWTVQEILNAEQPKQRAEIITHFIKVAKKLHELNNLHSLFAIISAMQSASIYRLTKTWACLSKKDRNAFDRLSDIFSDQDNWANLRSYLESLRLPCIPYLGLFLTDLIYIDLAHPHKGGLEPEQRRNKMNNILRVISNYQQSNYKHLQKHEATQKYLTSIRYIEELQNIFEEDQYKRSLNLEPASPSGPSSSSCSSKESFNVEVVTPALGCLNLSPAKTIGSMRMASGTKFVPGHRKCRSLGTKFRSSSLPRNFAEKCQCCIVMIAPGIGTISNKRCRCRRIFGKIATHNHSDGHPHHLHLELDPSQVQPRHHLLDDSVLEHSDALSQADLTSLESAEGIMCDFTGSDCDLMSPEAAAAMHGCVRRKTVQKEGRKPAVASWQRYWLQIWANSLVYFPPKSFKGSERNDFKREPCKVCPLDGWYAHVSDNTKHKNTFELCHRTLGTVYRFRTDSPQMTHLWSNAICKLATMRVPKPLPTNLMSFE

Target ORF information:

RefSeq Version	NM_166194.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166194.2

ORF Insert Sequence:

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAAATTTC GCAGCAGCAG CCTGCCGCGA AACTTTGCCG AGAAGTGCCA ATGTTGCATC 
GTCATGATTG CGCCGGGCAT CGGCACCATC TCCAACAAGC GTTGCAGATG CCGTCGCATT 
TTTGGCAAGA TTGCAACGCA CAACCACAGC GATGGACATC CTCACCACCT GCACCTGGAA 
CTTGATCCGA GTCAAGTGCA GCCTCGTCAC CACCTGCTAG ATGATTCGGT CTTGGAGCAC 
AGCGATGCCC TATCCCAAGC AGATCTAACA TCGTTGGAAA GTGCAGAGGG AATTATGTGT 
GATTTTACCG GCAGCGATTG CGATTTGATG TCACCAGAGG CAGCAGCCGC CATGCACGGA 
TGTGTCCGGC GTAAAACAGT TCAAAAGGAG GGAAGGAAGC CAGCGGTGGC ATCTTGGCAA 
CGATACTGGC TACAGATTTG GGCCAACTCG CTGGTGTATT TCCCACCCAA ATCCTTTAAA 
GGAAGCGAAC GCAACGACTT TAAGCGGGAA CCGTGCAAAG TGTGTCCATT GGATGGATGG 
TATGCCCACG TGAGCGATAA CACCAAGCAC AAGAACACCT TCGAACTGTG CCACCGAACT 
CTGGGCACCG TCTATCGCTT CCGGACGGAC AGTCCCCAGA TGACGCATCT CTGGTCGAAT 
GCCATTTGCA AATTGGCCAC CATGCGAGTG CCCAAACCTC TGCCCACCAA CCTAATGTCC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5522 ( NM_166195.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725615.1 Drosophila melanogaster uncharacterized protein, transcript variant E (CG5522), mRNA.

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CloneID	OFa10272
Clone ID Related Accession (Same CDS sequence)	NM_166193.2 , NM_166194.2 , NM_166195.2
Accession Version	NM_166195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166195.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAAATTTC GCAGCAGCAG CCTGCCGCGA AACTTTGCCG AGAAGTGCCA ATGTTGCATC 
GTCATGATTG CGCCGGGCAT CGGCACCATC TCCAACAAGC GTTGCAGATG CCGTCGCATT 
TTTGGCAAGA TTGCAACGCA CAACCACAGC GATGGACATC CTCACCACCT GCACCTGGAA 
CTTGATCCGA GTCAAGTGCA GCCTCGTCAC CACCTGCTAG ATGATTCGGT CTTGGAGCAC 
AGCGATGCCC TATCCCAAGC AGATCTAACA TCGTTGGAAA GTGCAGAGGG AATTATGTGT 
GATTTTACCG GCAGCGATTG CGATTTGATG TCACCAGAGG CAGCAGCCGC CATGCACGGA 
TGTGTCCGGC GTAAAACAGT TCAAAAGGAG GGAAGGAAGC CAGCGGTGGC ATCTTGGCAA 
CGATACTGGC TACAGATTTG GGCCAACTCG CTGGTGTATT TCCCACCCAA ATCCTTTAAA 
GGAAGCGAAC GCAACGACTT TAAGCGGGAA CCGTGCAAAG TGTGTCCATT GGATGGATGG 
TATGCCCACG TGAGCGATAA CACCAAGCAC AAGAACACCT TCGAACTGTG CCACCGAACT 
CTGGGCACCG TCTATCGCTT CCGGACGGAC AGTCCCCAGA TGACGCATCT CTGGTCGAAT 
GCCATTTGCA AATTGGCCAC CATGCGAGTG CCCAAACCTC TGCCCACCAA CCTAATGTCC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725615.1
CDS	398..2506
Translation	MMRYSEISRDLSTDNLRYSELSRKPTTDYNGYTGQAYAATQFKQSSPPPPPSQQQQQPTRVSKQRSPNTNGSAHTMACLRQEKTYKEQQMEPPSMQPQASKTLNIKSTRRKNSIGCLNSFSSSPDSPGCYYTIAASAAPPSKSQSLPAHASIKQLDAVILSALRVPADVLANQITLLDFPVFAQIQPDELSSCAWTKKDKHVNTPNIVAFTKRFNHTSFWTVQEILNAEQPKQRAEIITHFIKVAKKLHELNNLHSLFAIISAMQSASIYRLTKTWACLSKKDRNAFDRLSDIFSDQDNWANLRSYLESLRLPCIPYLGLFLTDLIYIDLAHPHKGGLEPEQRRNKMNNILRVISNYQQSNYKHLQKHEATQKYLTSIRYIEELQNIFEEDQYKRSLNLEPASPSGPSSSSCSSKESFNVEVVTPALGCLNLSPAKTIGSMRMASGTKFVPGHRKCRSLGTKFRSSSLPRNFAEKCQCCIVMIAPGIGTISNKRCRCRRIFGKIATHNHSDGHPHHLHLELDPSQVQPRHHLLDDSVLEHSDALSQADLTSLESAEGIMCDFTGSDCDLMSPEAAAAMHGCVRRKTVQKEGRKPAVASWQRYWLQIWANSLVYFPPKSFKGSERNDFKREPCKVCPLDGWYAHVSDNTKHKNTFELCHRTLGTVYRFRTDSPQMTHLWSNAICKLATMRVPKPLPTNLMSFE

Target ORF information:

RefSeq Version	NM_166195.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant E (CG5522), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166195.2

ORF Insert Sequence:

ATGATGCGAT ACTCGGAAAT CTCACGCGAC CTGTCCACGG ATAATCTGCG CTACTCAGAG 
TTAAGCAGAA AGCCCACCAC GGACTATAAT GGATATACGG GTCAGGCCTA TGCCGCGACT 
CAATTCAAGC AGAGCTCACC TCCTCCTCCT CCTTCACAAC AGCAGCAGCA GCCAACTCGG 
GTGTCCAAGC AACGAAGTCC GAATACCAAT GGTAGTGCCC ACACCATGGC CTGTTTGCGC 
CAGGAGAAGA CCTACAAGGA GCAGCAAATG GAACCGCCCA GTATGCAACC GCAGGCCAGC 
AAAACTCTAA ATATCAAGAG CACGCGCCGC AAGAATTCCA TCGGTTGTTT GAATAGTTTC 
TCCTCCTCGC CAGATTCGCC TGGTTGCTAC TACACTATAG CAGCCTCGGC AGCACCACCG 
TCCAAGTCGC AAAGTTTACC AGCCCACGCC TCCATCAAGC AGTTGGATGC GGTTATTTTG 
AGCGCGTTAC GTGTACCCGC CGATGTGTTG GCTAATCAGA TTACGCTACT GGATTTCCCA 
GTCTTTGCCC AGATCCAACC GGATGAGCTG AGCAGTTGTG CGTGGACGAA AAAGGATAAG 
CATGTCAATA CGCCGAACAT TGTGGCATTT ACTAAGCGAT TTAACCACAC GAGCTTTTGG 
ACAGTCCAGG AGATTCTCAA TGCCGAGCAG CCAAAGCAGA GAGCCGAGAT TATTACACAC 
TTTATAAAGG TGGCCAAAAA ACTCCACGAG CTCAACAATC TGCACTCGCT GTTTGCCATT 
ATTTCGGCCA TGCAAAGCGC CAGCATATAT CGCCTAACTA AAACCTGGGC TTGCTTATCG 
AAAAAGGATC GAAATGCATT CGATCGCCTC AGCGATATAT TCAGTGATCA GGACAACTGG 
GCGAATCTGC GCTCCTACCT CGAGAGCCTC CGCCTGCCCT GCATCCCCTA TCTGGGTCTC 
TTTCTCACCG ATCTGATCTA CATCGATTTG GCCCACCCAC ATAAGGGCGG ATTGGAGCCA 
GAGCAGCGTC GCAATAAGAT GAACAATATA TTGCGTGTAA TTTCGAACTA TCAACAGTCG 
AACTATAAGC ATTTGCAGAA GCATGAGGCG ACACAAAAGT ATTTGACTTC GATAAGGTAT 
ATAGAGGAGT TGCAGAACAT ATTCGAGGAG GATCAGTACA AGCGCTCTCT AAACCTGGAA 
CCTGCGTCGC CCTCTGGACC CAGTTCTTCG TCCTGCAGCT CAAAAGAGTC CTTCAATGTG 
GAAGTAGTTA CTCCGGCCCT TGGATGCCTA AATCTCTCGC CGGCCAAAAC CATTGGCTCC 
ATGCGAATGG CAAGCGGCAC AAAATTTGTG CCAGGCCATC GCAAGTGTCG CAGTCTGGGC 
ACCAAATTTC GCAGCAGCAG CCTGCCGCGA AACTTTGCCG AGAAGTGCCA ATGTTGCATC 
GTCATGATTG CGCCGGGCAT CGGCACCATC TCCAACAAGC GTTGCAGATG CCGTCGCATT 
TTTGGCAAGA TTGCAACGCA CAACCACAGC GATGGACATC CTCACCACCT GCACCTGGAA 
CTTGATCCGA GTCAAGTGCA GCCTCGTCAC CACCTGCTAG ATGATTCGGT CTTGGAGCAC 
AGCGATGCCC TATCCCAAGC AGATCTAACA TCGTTGGAAA GTGCAGAGGG AATTATGTGT 
GATTTTACCG GCAGCGATTG CGATTTGATG TCACCAGAGG CAGCAGCCGC CATGCACGGA 
TGTGTCCGGC GTAAAACAGT TCAAAAGGAG GGAAGGAAGC CAGCGGTGGC ATCTTGGCAA 
CGATACTGGC TACAGATTTG GGCCAACTCG CTGGTGTATT TCCCACCCAA ATCCTTTAAA 
GGAAGCGAAC GCAACGACTT TAAGCGGGAA CCGTGCAAAG TGTGTCCATT GGATGGATGG 
TATGCCCACG TGAGCGATAA CACCAAGCAC AAGAACACCT TCGAACTGTG CCACCGAACT 
CTGGGCACCG TCTATCGCTT CCGGACGGAC AGTCCCCAGA TGACGCATCT CTGGTCGAAT 
GCCATTTGCA AATTGGCCAC CATGCGAGTG CCCAAACCTC TGCCCACCAA CCTAATGTCC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5522 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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