unc-104 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-104
Entrez Gene ID	36876
Full Name	uncoordinated-104
Synonyms	CG8566,DUNC104,DUnc-104,DmCG8566,DmDUnc104,DmKlp53D,Dmel\CG8566,Dunc104,Imac,KIF 1B,KIF1A,KIF1A/1B,Klp53D,Unc-104,Unc104,bris,imac,unc104
General protein information	Preferred Names uncoordinated-104 Names CG8566 gene product from transcript CG8566-RB CG8566-PB CG8566-PC CG8566-PD CG8566-PE CG8566-PF CG8566-PG CG8566-PH CG8566-PI D uncoordinated protein 104 Kinesin-3 Kinesin4B bristly immaculate connections kinesin 3 unc-104 ortholog unc-104-PB unc-104-PC unc-104-PD unc-104-PE unc-104-PF unc-104-PG unc-104-PH unc-104-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53D6-53D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259444.2	NP_001246373.1	uncoordinated-104, isoform F
NM_001316424.1	NP_001303353.1	uncoordinated-104, isoform I
NM_166190.4	NP_725607.2	uncoordinated-104, isoform C
NM_001259445.2	NP_001246374.1	uncoordinated-104, isoform G
NM_001274104.2	NP_001261033.1	uncoordinated-104, isoform H
NM_137311.5	NP_611155.3	uncoordinated-104, isoform D
NM_166192.4	NP_725610.2	uncoordinated-104, isoform B
NM_001103876.3	NP_001097346.1	uncoordinated-104, isoform E

Pathways from BioSystems

Homologies

	KIF1A	XP_002799156.1
Gallus gallus (chicken)	KIF1A	XP_003641781.1
Rattus norvegicus (Norway rat)	Kif1a	XP_006245623.1
Drosophila melanogaster (fruit fly)	unc-104	NP_001246373.1
Anopheles gambiae (African malaria mosquito)	KIF1A_ANOGA	XP_314493.3
Pan troglodytes (chimpanzee)	KIF1A	XP_003309612.1
Bos taurus (cattle)	KIF1A	XP_005205116.1
Mus musculus (house mouse)	Kif1a	NP_032466.2
Caenorhabditis elegans (roundworm)	unc-104	NP_001022041.1
Homo sapiens (human)	KIF1A	NP_004312.2
Canis lupus familiaris (dog)	KIF1A	NP_001161945.1
Danio rerio (zebrafish)	kif1a	XP_005166002.1
Danio rerio (zebrafish)	kif1ab	XP_005171271.1
Xenopus tropicalis (tropical clawed frog)	kif1a	XP_002933380.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Unc-104 critically restricts neuromuscular junction growth and dendrite branch morphogenesis.
Title: The kinesin-3, unc-104 regulates dendrite morphogenesis and synaptic development in Drosophila.

Drosophila Unc-104 has neuronal functions that are distinct from those of the classic anterograde axonal motor, kinesin-1.
Title: Identification of an axonal kinesin-3 motor for fast anterograde vesicle transport that facilitates retrograde transport of neuropeptides.

Our data thus indicate that Imac transports components required for synaptic maturation and provide insight into presynaptic maturation as a process that can be differentiated from axon outgrowth and targeting.
Title: A Drosophila kinesin required for synaptic bouton formation and synaptic vesicle transport.

The following unc-104 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-104 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa41308	NM_001316424.1 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFa10264	NM_166192.4 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa10264	NM_166190.4 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa10265	NM_137311.5 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa10266	NM_001103876.3 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12366	NM_001274104.2 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12369	NM_001259445.2 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12371	NM_001259444.2 Latest version!	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-104 ( NM_001316424.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303353.1 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa41308
Clone ID Related Accession (Same CDS sequence)	NM_001316424.1
Accession Version	NM_001316424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA ATTATGTTCA CCGGATCGAG CTGATGCACC CAATGGCTGG 
GAGGCACCTG CTCCGGCTAC TCAGCCGGCT CTTCCCCTCC GTCTCTATGT GCCGGAACTG 
GAGGAGATTC GCGTGAGTCC TGTGGTGGCC CGCAAGGGTC TGTTGAATGT CCTGGAGCAC 
GGCGGTTCCG GCTGGAAGAA GCGCTGGGTG ATCGTCCGTC GCCCGTACGT GTTTATCTAC 
CGCTCGGAGA AGGATCCCGT TGAACGGGCT GTCCTCAATC TGGCGACTGC GCATGTGGAG 
TGCAGCGAGG ACCAGGCGGC CATGGTCAAG ATACCCAACA CATTCAGTGT GGTGACCAAG 
CATCGTGGCT ATCTGCTGCA AACTCTCGGT GACAAGGAAG TACACGACTG GCTGTATGCT 
ATCAATCCAT TGCTTGCTGG GCAGATAAAA TCCCGCCTGG CGCGACGGAC TTTGGAGCCT 
GCTAGCCAGA CGGCTTCCCA GATCCAGGCC ACCAATGCGG CGAATGCCAA TAGTGCGAGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303353.1
CDS	291..5276
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEEDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMELCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK

Target ORF information:

RefSeq Version	NM_001316424.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316424.1

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA ATTATGTTCA CCGGATCGAG CTGATGCACC CAATGGCTGG 
GAGGCACCTG CTCCGGCTAC TCAGCCGGCT CTTCCCCTCC GTCTCTATGT GCCGGAACTG 
GAGGAGATTC GCGTGAGTCC TGTGGTGGCC CGCAAGGGTC TGTTGAATGT CCTGGAGCAC 
GGCGGTTCCG GCTGGAAGAA GCGCTGGGTG ATCGTCCGTC GCCCGTACGT GTTTATCTAC 
CGCTCGGAGA AGGATCCCGT TGAACGGGCT GTCCTCAATC TGGCGACTGC GCATGTGGAG 
TGCAGCGAGG ACCAGGCGGC CATGGTCAAG ATACCCAACA CATTCAGTGT GGTGACCAAG 
CATCGTGGCT ATCTGCTGCA AACTCTCGGT GACAAGGAAG TACACGACTG GCTGTATGCT 
ATCAATCCAT TGCTTGCTGG GCAGATAAAA TCCCGCCTGG CGCGACGGAC TTTGGAGCCT 
GCTAGCCAGA CGGCTTCCCA GATCCAGGCC ACCAATGCGG CGAATGCCAA TAGTGCGAGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-104 ( NM_166190.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725607.2 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10264
Clone ID Related Accession (Same CDS sequence)	NM_166190.4 , NM_166192.4
Accession Version	NM_166190.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166190.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA AAACAACAAA TTTGTAATTC GACGCAGATT ATGTTCACCG 
GATCGAGCTG ATGCACCCAA TGGCTGGGAG GCACCTGCTC CGGCTACTCA GCCGGCTCTT 
CCCCTCCGTC TCTATGTGCC GGAACTGGAG GAGATTCGCG TGAGTCCTGT GGTGGCCCGC 
AAGGGTCTGT TGAATGTCCT GGAGCACGGC GGTTCCGGCT GGAAGAAGCG CTGGGTGATC 
GTCCGTCGCC CGTACGTGTT TATCTACCGC TCGGAGAAGG ATCCCGTTGA ACGGGCTGTC 
CTCAATCTGG CGACTGCGCA TGTGGAGTGC AGCGAGGACC AGGCGGCCAT GGTCAAGATA 
CCCAACACAT TCAGTGTGGT GACCAAGCAT CGTGGCTATC TGCTGCAAAC TCTCGGTGAC 
AAGGAAGTAC ACGACTGGCT GTATGCTATC AATCCATTGC TTGCTGGGCA GATAAAATCC 
CGCCTGGCGC GACGGACTTT GGAGCCTGCT AGCCAGACGG CTTCCCAGAT CCAGGCCACC 
AATGCGGCGA ATGCCAATAG TGCGAGCAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725607.2
CDS	449..5461
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEEDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMENNKFVIRRRLCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK

Target ORF information:

RefSeq Version	NM_166190.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166190.4

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA AAACAACAAA TTTGTAATTC GACGCAGATT ATGTTCACCG 
GATCGAGCTG ATGCACCCAA TGGCTGGGAG GCACCTGCTC CGGCTACTCA GCCGGCTCTT 
CCCCTCCGTC TCTATGTGCC GGAACTGGAG GAGATTCGCG TGAGTCCTGT GGTGGCCCGC 
AAGGGTCTGT TGAATGTCCT GGAGCACGGC GGTTCCGGCT GGAAGAAGCG CTGGGTGATC 
GTCCGTCGCC CGTACGTGTT TATCTACCGC TCGGAGAAGG ATCCCGTTGA ACGGGCTGTC 
CTCAATCTGG CGACTGCGCA TGTGGAGTGC AGCGAGGACC AGGCGGCCAT GGTCAAGATA 
CCCAACACAT TCAGTGTGGT GACCAAGCAT CGTGGCTATC TGCTGCAAAC TCTCGGTGAC 
AAGGAAGTAC ACGACTGGCT GTATGCTATC AATCCATTGC TTGCTGGGCA GATAAAATCC 
CGCCTGGCGC GACGGACTTT GGAGCCTGCT AGCCAGACGG CTTCCCAGAT CCAGGCCACC 
AATGCGGCGA ATGCCAATAG TGCGAGCAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-104 ( NM_166192.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725610.2 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10264
Clone ID Related Accession (Same CDS sequence)	NM_166190.4 , NM_166192.4
Accession Version	NM_166192.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166192.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA AAACAACAAA TTTGTAATTC GACGCAGATT ATGTTCACCG 
GATCGAGCTG ATGCACCCAA TGGCTGGGAG GCACCTGCTC CGGCTACTCA GCCGGCTCTT 
CCCCTCCGTC TCTATGTGCC GGAACTGGAG GAGATTCGCG TGAGTCCTGT GGTGGCCCGC 
AAGGGTCTGT TGAATGTCCT GGAGCACGGC GGTTCCGGCT GGAAGAAGCG CTGGGTGATC 
GTCCGTCGCC CGTACGTGTT TATCTACCGC TCGGAGAAGG ATCCCGTTGA ACGGGCTGTC 
CTCAATCTGG CGACTGCGCA TGTGGAGTGC AGCGAGGACC AGGCGGCCAT GGTCAAGATA 
CCCAACACAT TCAGTGTGGT GACCAAGCAT CGTGGCTATC TGCTGCAAAC TCTCGGTGAC 
AAGGAAGTAC ACGACTGGCT GTATGCTATC AATCCATTGC TTGCTGGGCA GATAAAATCC 
CGCCTGGCGC GACGGACTTT GGAGCCTGCT AGCCAGACGG CTTCCCAGAT CCAGGCCACC 
AATGCGGCGA ATGCCAATAG TGCGAGCAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725610.2
CDS	458..5470
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEEDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMENNKFVIRRRLCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK

Target ORF information:

RefSeq Version	NM_166192.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166192.4

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA AAACAACAAA TTTGTAATTC GACGCAGATT ATGTTCACCG 
GATCGAGCTG ATGCACCCAA TGGCTGGGAG GCACCTGCTC CGGCTACTCA GCCGGCTCTT 
CCCCTCCGTC TCTATGTGCC GGAACTGGAG GAGATTCGCG TGAGTCCTGT GGTGGCCCGC 
AAGGGTCTGT TGAATGTCCT GGAGCACGGC GGTTCCGGCT GGAAGAAGCG CTGGGTGATC 
GTCCGTCGCC CGTACGTGTT TATCTACCGC TCGGAGAAGG ATCCCGTTGA ACGGGCTGTC 
CTCAATCTGG CGACTGCGCA TGTGGAGTGC AGCGAGGACC AGGCGGCCAT GGTCAAGATA 
CCCAACACAT TCAGTGTGGT GACCAAGCAT CGTGGCTATC TGCTGCAAAC TCTCGGTGAC 
AAGGAAGTAC ACGACTGGCT GTATGCTATC AATCCATTGC TTGCTGGGCA GATAAAATCC 
CGCCTGGCGC GACGGACTTT GGAGCCTGCT AGCCAGACGG CTTCCCAGAT CCAGGCCACC 
AATGCGGCGA ATGCCAATAG TGCGAGCAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-104 ( NM_137311.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611155.3 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant D, mRNA.

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CloneID	OFa10265
Clone ID Related Accession (Same CDS sequence)	NM_137311.5
Accession Version	NM_137311.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137311.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGCTAT CGCCTCGAGC TGATGAGACA AATATACAAT 
GTCGAGAGCC CACCATCGTC CATGCTGTTC GATACGTCCG GCATGGAGGC ATTGAGCGGA 
TGGATCTCAC CACCCAGCCA ACATCCTGGC CAGCAGGCGC AGCTGCTCCC ATTGGAGCCA 
CCAGTCGAAA GCGAGCGTGG TCGTCTCACA TTGGCCAATC TGATACCTTC TAGGCAACGT 
TTGGAGCTGA TGCGCGAGAT GTACCACAAC GAAGCCGAAA TGAGTCCCAC TTCACCGGAC 
TATAATGTGG AGAGCCTCAC TGGTGGAGAT CCATTCTACG ATCGCTTCCC CTGGTTCCGC 
ATGGTGGGTC GCTCCTTCAT CTATCTGAGC AACCTGCTCT ACCCAGTGCC ATTGGTCCAC 
AAGGTGGCCA TTGTCAACGA ACGCGGAGAT GTGCGTGGCT ACCTAAGGAT TGCTGTGCAG 
CCCGTTCTGG ATGAGGAGTC CATTGATTTC AATAATGGTG TCAAGCAGTC AGCTCGCTTG 
GTTTTCAATG AGGATGATGC CAAACCGAAA TACCGAGCTC TCAATGAAAA GGATGATGTG 
CAGCGCTATA TTGACAATGG TGGTTTGGAC AGCAAGCTGG AGGAACTTGA GGATGTGGAC 
TCTGGTCGTG GCATTGACTC TAACTCCGCA TCCGAGTGCC ACGAAAATTC GGAGGAGCCT 
GGCGAGCATC TGCAGGTGGG CAAGGAGTTC ACTTTCCGGG TCACTGTGCT ACAGGCCACT 
GGTATTGGGG CTGAATATGC CGATATCTTC TGCCAGTTCA ACTTTTTGCA TCGTCATGAG 
GAGGCTTTCT CCACCGAACC CGTCAAGAAT TCGGCATCGG GTGCTCCTCT AGGCTTCTAC 
CATGTGCAGA ACATAACTGT ACCCGTAACC AAATCCTTCA TCGAGTATTT GAAGACCCAA 
CCCATAATGT TCAAGATATT TGGGCACTAC CAGACGCACC CATTGCATAA GGATGCCAAA 
CAGGATTTTG TTTCTCGGCC ACCACCACGT CGCATGTTGC CACCGAGCAT ACCGATCAGT 
CAGCCAGTGC GTAGTCCCAA GTTTGGACCA CTACCGTGTG CACCCACGTC TACGGTTTTG 
GCTAAGCACG ATGTTTTGGT TTGGTTTGAA ATCTGTGAAT TGGCTCCCAA TGGAGAATAT 
GTGCCATCGG TGGTGGAGCA CAGCGATGAT CTTCCCTGCC GCGGACTGTT CCTCTTGCAT 
CAGGGCATCC AGCGGCGCAT TCGTATTACT ATCGTACATG AGCCCACAAC CGAGGTGAAG 
TGGAAGGACA TCAATGAGTT GGTGGTTGGA CGTATCCGCA ATACACCGGA GTCATCCGAC 
GAACAGGACG AAGACGCCTG TGTCTTATCT TTGGGCTTGT TCCCAGGCGA GGCTTTGGAG 
GTGCCCGGAG ATGATAGATC TTTCTACCGT TTTGAGGCTG CCTGGGACTC GAGTCTGCAC 
AACTCGGCAC TGCTCAACCG CGTTTCTCAA GGCGGTGAGA CCATCTACAT CACCCTGAGC 
GCTTACTTGG AGCTGGAGAA TTGCGCCCGC CCGGCCATAA TTACCAAGGA CCTGAGCATG 
GTTATCTATG GACGCGATGC CCGCACCGGT CCGCGCTCCC TGAAGCACCT GTTTTCGGGA 
CAGTACCGCA ATCCGGAGGC CAATCGCCTA ACCGGGGTTT ACGAGCTAGC ACTGCGCAGA 
GCATCCGAAG CAGGTAGTCC AGGTGTACAA AGGCGTCAAC GTCGAGTGCT GGACACCAGC 
TCTACTTATG TGCGCGGCGA GGAGAATCTT CATGGCTGGA GGCCAAGGGG TGACTCCCTG 
ATCTTCGACC ACCAGTGGGA GCTGGAGAAA CTCACCAGAC TTGAAGAGGT TGGACGCATG 
CGGCACTTGC TTCTGCTGCG CGAACGTCTG GGCATGGACA CCAACCCAAA TCCGACCACC 
AAGACCGAGA AGGATGTATG CAATCTAGCT GCTCGGGCAG CCACATCACC CGTACATATG 
GTCATTCCAC AATCGCCGCA GACTCCGGTC AAGGACCCAC AGCAAATCAT TCCAGAACGC 
GAATACAACC AACGGGAGCA GGATCTCATG CTTAAATGCT TAAAGTTGGT GCAGGGACGC 
TATACTAAGA GCGAGGCCAA CGATACGCAA ACTCAGTCGG ATGTTTCGCC TAGCGATGAG 
GGATGCGCCG ACATGACCGT CAGCTGCATC TCCAGCAATT CCATGGAAAA CAACAAATTT 
GTAATTCGAC GCAGATTATG TTCACCGGAT CGAGCTGATG CACCCAATGG CTGGGAGGCA 
CCTGCTCCGG CTACTCAGCC GGCTCTTCCC CTCCGTCTCT ATGTGCCGGA ACTGGAGGAG 
ATTCGCGTGA GTCCTGTGGT GGCCCGCAAG GGTCTGTTGA ATGTCCTGGA GCACGGCGGT 
TCCGGCTGGA AGAAGCGCTG GGTGATCGTC CGTCGCCCGT ACGTGTTTAT CTACCGCTCG 
GAGAAGGATC CCGTTGAACG GGCTGTCCTC AATCTGGCGA CTGCGCATGT GGAGTGCAGC 
GAGGACCAGG CGGCCATGGT CAAGATACCC AACACATTCA GTGTGGTGAC CAAGCATCGT 
GGCTATCTGC TGCAAACTCT CGGTGACAAG GAAGTACACG ACTGGCTGTA TGCTATCAAT 
CCATTGCTTG CTGGGCAGAT AAAATCCCGC CTGGCGCGAC GGACTTTGGA GCCTGCTAGC 
CAGACGGCTT CCCAGATCCA GGCCACCAAT GCGGCGAATG CCAATAGTGC GAGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611155.3
CDS	449..5668
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEEDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRYRLELMRQIYNVESPPSSMLFDTSGMEALSGWISPPSQHPGQQAQLLPLEPPVESERGRLTLANLIPSRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMENNKFVIRRRLCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK

Target ORF information:

RefSeq Version	NM_137311.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137311.5

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGCTAT CGCCTCGAGC TGATGAGACA AATATACAAT 
GTCGAGAGCC CACCATCGTC CATGCTGTTC GATACGTCCG GCATGGAGGC ATTGAGCGGA 
TGGATCTCAC CACCCAGCCA ACATCCTGGC CAGCAGGCGC AGCTGCTCCC ATTGGAGCCA 
CCAGTCGAAA GCGAGCGTGG TCGTCTCACA TTGGCCAATC TGATACCTTC TAGGCAACGT 
TTGGAGCTGA TGCGCGAGAT GTACCACAAC GAAGCCGAAA TGAGTCCCAC TTCACCGGAC 
TATAATGTGG AGAGCCTCAC TGGTGGAGAT CCATTCTACG ATCGCTTCCC CTGGTTCCGC 
ATGGTGGGTC GCTCCTTCAT CTATCTGAGC AACCTGCTCT ACCCAGTGCC ATTGGTCCAC 
AAGGTGGCCA TTGTCAACGA ACGCGGAGAT GTGCGTGGCT ACCTAAGGAT TGCTGTGCAG 
CCCGTTCTGG ATGAGGAGTC CATTGATTTC AATAATGGTG TCAAGCAGTC AGCTCGCTTG 
GTTTTCAATG AGGATGATGC CAAACCGAAA TACCGAGCTC TCAATGAAAA GGATGATGTG 
CAGCGCTATA TTGACAATGG TGGTTTGGAC AGCAAGCTGG AGGAACTTGA GGATGTGGAC 
TCTGGTCGTG GCATTGACTC TAACTCCGCA TCCGAGTGCC ACGAAAATTC GGAGGAGCCT 
GGCGAGCATC TGCAGGTGGG CAAGGAGTTC ACTTTCCGGG TCACTGTGCT ACAGGCCACT 
GGTATTGGGG CTGAATATGC CGATATCTTC TGCCAGTTCA ACTTTTTGCA TCGTCATGAG 
GAGGCTTTCT CCACCGAACC CGTCAAGAAT TCGGCATCGG GTGCTCCTCT AGGCTTCTAC 
CATGTGCAGA ACATAACTGT ACCCGTAACC AAATCCTTCA TCGAGTATTT GAAGACCCAA 
CCCATAATGT TCAAGATATT TGGGCACTAC CAGACGCACC CATTGCATAA GGATGCCAAA 
CAGGATTTTG TTTCTCGGCC ACCACCACGT CGCATGTTGC CACCGAGCAT ACCGATCAGT 
CAGCCAGTGC GTAGTCCCAA GTTTGGACCA CTACCGTGTG CACCCACGTC TACGGTTTTG 
GCTAAGCACG ATGTTTTGGT TTGGTTTGAA ATCTGTGAAT TGGCTCCCAA TGGAGAATAT 
GTGCCATCGG TGGTGGAGCA CAGCGATGAT CTTCCCTGCC GCGGACTGTT CCTCTTGCAT 
CAGGGCATCC AGCGGCGCAT TCGTATTACT ATCGTACATG AGCCCACAAC CGAGGTGAAG 
TGGAAGGACA TCAATGAGTT GGTGGTTGGA CGTATCCGCA ATACACCGGA GTCATCCGAC 
GAACAGGACG AAGACGCCTG TGTCTTATCT TTGGGCTTGT TCCCAGGCGA GGCTTTGGAG 
GTGCCCGGAG ATGATAGATC TTTCTACCGT TTTGAGGCTG CCTGGGACTC GAGTCTGCAC 
AACTCGGCAC TGCTCAACCG CGTTTCTCAA GGCGGTGAGA CCATCTACAT CACCCTGAGC 
GCTTACTTGG AGCTGGAGAA TTGCGCCCGC CCGGCCATAA TTACCAAGGA CCTGAGCATG 
GTTATCTATG GACGCGATGC CCGCACCGGT CCGCGCTCCC TGAAGCACCT GTTTTCGGGA 
CAGTACCGCA ATCCGGAGGC CAATCGCCTA ACCGGGGTTT ACGAGCTAGC ACTGCGCAGA 
GCATCCGAAG CAGGTAGTCC AGGTGTACAA AGGCGTCAAC GTCGAGTGCT GGACACCAGC 
TCTACTTATG TGCGCGGCGA GGAGAATCTT CATGGCTGGA GGCCAAGGGG TGACTCCCTG 
ATCTTCGACC ACCAGTGGGA GCTGGAGAAA CTCACCAGAC TTGAAGAGGT TGGACGCATG 
CGGCACTTGC TTCTGCTGCG CGAACGTCTG GGCATGGACA CCAACCCAAA TCCGACCACC 
AAGACCGAGA AGGATGTATG CAATCTAGCT GCTCGGGCAG CCACATCACC CGTACATATG 
GTCATTCCAC AATCGCCGCA GACTCCGGTC AAGGACCCAC AGCAAATCAT TCCAGAACGC 
GAATACAACC AACGGGAGCA GGATCTCATG CTTAAATGCT TAAAGTTGGT GCAGGGACGC 
TATACTAAGA GCGAGGCCAA CGATACGCAA ACTCAGTCGG ATGTTTCGCC TAGCGATGAG 
GGATGCGCCG ACATGACCGT CAGCTGCATC TCCAGCAATT CCATGGAAAA CAACAAATTT 
GTAATTCGAC GCAGATTATG TTCACCGGAT CGAGCTGATG CACCCAATGG CTGGGAGGCA 
CCTGCTCCGG CTACTCAGCC GGCTCTTCCC CTCCGTCTCT ATGTGCCGGA ACTGGAGGAG 
ATTCGCGTGA GTCCTGTGGT GGCCCGCAAG GGTCTGTTGA ATGTCCTGGA GCACGGCGGT 
TCCGGCTGGA AGAAGCGCTG GGTGATCGTC CGTCGCCCGT ACGTGTTTAT CTACCGCTCG 
GAGAAGGATC CCGTTGAACG GGCTGTCCTC AATCTGGCGA CTGCGCATGT GGAGTGCAGC 
GAGGACCAGG CGGCCATGGT CAAGATACCC AACACATTCA GTGTGGTGAC CAAGCATCGT 
GGCTATCTGC TGCAAACTCT CGGTGACAAG GAAGTACACG ACTGGCTGTA TGCTATCAAT 
CCATTGCTTG CTGGGCAGAT AAAATCCCGC CTGGCGCGAC GGACTTTGGA GCCTGCTAGC 
CAGACGGCTT CCCAGATCCA GGCCACCAAT GCGGCGAATG CCAATAGTGC GAGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-104 ( NM_001103876.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097346.1 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant E, mRNA.

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CloneID	OFa10266
Clone ID Related Accession (Same CDS sequence)	NM_001103876.3
Accession Version	NM_001103876.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103876.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGGA CCCGATGGCA AAGTGGTGTG TGAGAAGCGC 
GATGCGAATA AGGATGAGCT CACCAAGTCA ACGGTGATCA AGTCGCCCAC TAAGTCACGT 
AATCGTAATG GATCCACCAC GGAGATGGCT GTGGATCAGC TGCAGGCCAG CGAGAAACTT 
ATTGCAGAGC TCAACGAGAC CTGGGAGGAG AAACTTAAGC GCACCGAGGA GATTCGCGTG 
CAGCGAGAGG CGGTCTTCGC CGAAATGGGT GTGGCTGTCA AGGAAGATGG AATAACAGTT 
GGCGTATTCT CACCGAAGAA GACTCCGCAT TTGGTCAACC TAAACGAGGA TCCCAATCTG 
TCTGAGTGTC TGCTTTACTA CATCAAGGAG GGTCTAACTC GGTTGGGTAC CCATGAAGCA 
AATGTGCCCC AGGACATTCA GCTCTCCGGA TCGCACATCC TCAAAGAGCA CTGCACCTTT 
GAGAACAAGA ACAGCACGGT TACCCTGCTG CCACACAAGG ATGCTATCAT CTATGTAAAT 
GGACGCAAGT TGGTTGAACC GGAGGTTCTT AAGACCGGTT CTCGCGTGAT CCTCGGAAAG 
AACCACGTGT TCCGCTTTAC CAATCCAGAA CAGGCACGCG AATTACGGGA TAAGATCGAG 
ACCGAAAATG AGGCTGAGAA CGAAGTGGAG AAGACAGACA CCCAGCAGGT GGACTGGAAT 
TTTGCCCAGT GCGAATTGCT CGAGAAGCAA GGCATTGATC TTAAAGCTGA AATGAAGAAG 
CGTTTAGACA ACTTGGAGGA ACAATACAAG CGGGAGAAAC TTCAGGCCGA TCAGCAATTC 
GAGGAGCAGC GCAAAACGTA CGAGGCTCGC ATCGATGCTT TGCAAAAACA GGTTGAGGAG 
CAATCCATGA CCATGTCCAT GTACAGCAGC TACTCGCCGG AGGATTTCCA CCAGGAGGAG 
GACGTCTACA CCAACCCCAT GTACGAATCC TGCTGGACAG CAAGAGAGGC TGGCTTGGCT 
GCCTGGGCTT TCCGCAAGTG GCGTTACCAC CAATTCACCT CTTTGCGAGA TGATCTCTGG 
GGCAATGCTA TATTCCTTAA GGAAGCGAAT GCCATTTCCG TTGAGTTAAA GAAGAAGGTA 
CAATTCCAAT TTACTCTCTT GACCGACACC TTGTACTCTC CTTTGCCGCC TGAGCTGGCA 
TCCACTGTGG CTCCTGTGCA TCAGGAGGAT GAGTTCGGAG CTCCACCTGT CTCTAAGACC 
TTGGTGGCCG TCGAAGTAAC CGATACTAAG AACGGAGCCA CTCACCACTG GTCTCTGGAG 
AAGTTACGGC AACGTTTGGA GCTGATGCGC GAGATGTACC ACAACGAAGC CGAAATGAGT 
CCCACTTCAC CGGACTATAA TGTGGAGAGC CTCACTGGTG GAGATCCATT CTACGATCGC 
TTCCCCTGGT TCCGCATGGT GGGTCGCTCC TTCATCTATC TGAGCAACCT GCTCTACCCA 
GTGCCATTGG TCCACAAGGT GGCCATTGTC AACGAACGCG GAGATGTGCG TGGCTACCTA 
AGGATTGCTG TGCAGCCCGT TCTGGATGAG GAGTCCATTG ATTTCAATAA TGGTGTCAAG 
CAGTCAGCTC GCTTGGTTTT CAATGAGGAT GATGCCAAAC CGAAATACCG AGCTCTCAAT 
GAAAAGGATG ATGTGCAGCG CTATATTGAC AATGGTGGTT TGGACAGCAA GCTGGAGGAA 
CTTGAGGATG TGGACTCTGG TCGTGGCATT GACTCTAACT CCGCATCCGA GTGCCACGAA 
AATTCGGAGG AGCCTGGCGA GCATCTGCAG GTGGGCAAGG AGTTCACTTT CCGGGTCACT 
GTGCTACAGG CCACTGGTAT TGGGGCTGAA TATGCCGATA TCTTCTGCCA GTTCAACTTT 
TTGCATCGTC ATGAGGAGGC TTTCTCCACC GAACCCGTCA AGAATTCGGC ATCGGGTGCT 
CCTCTAGGCT TCTACCATGT GCAGAACATA ACTGTACCCG TAACCAAATC CTTCATCGAG 
TATTTGAAGA CCCAACCCAT AATGTTCAAG ATATTTGGGC ACTACCAGAC GCACCCATTG 
CATAAGGATG CCAAACAGGA TTTTGTTTCT CGGCCACCAC CACGTCGCAT GTTGCCACCG 
AGCATACCGA TCAGTCAGCC AGTGCGTAGT CCCAAGTTTG GACCACTACC GTGTGCACCC 
ACGTCTACGG TTTTGGCTAA GCACGATGTT TTGGTTTGGT TTGAAATCTG TGAATTGGCT 
CCCAATGGAG AATATGTGCC ATCGGTGGTG GAGCACAGCG ATGATCTTCC CTGCCGCGGA 
CTGTTCCTCT TGCATCAGGG CATCCAGCGG CGCATTCGTA TTACTATCGT ACATGAGCCC 
ACAACCGAGG TGAAGTGGAA GGACATCAAT GAGTTGGTGG TTGGACGTAT CCGCAATACA 
CCGGAGTCAT CCGACGAACA GGACGAAGAC GCCTGTGTCT TATCTTTGGG CTTGTTCCCA 
GGCGAGGCTT TGGAGGTGCC CGGAGATGAT AGATCTTTCT ACCGTTTTGA GGCTGCCTGG 
GACTCGAGTC TGCACAACTC GGCACTGCTC AACCGCGTTT CTCAAGGCGG TGAGACCATC 
TACATCACCC TGAGCGCTTA CTTGGAGCTG GAGAATTGCG CCCGCCCGGC CATAATTACC 
AAGGACCTGA GCATGGTTAT CTATGGACGC GATGCCCGCA CCGGTCCGCG CTCCCTGAAG 
CACCTGTTTT CGGGACAGTA CCGCAATCCG GAGGCCAATC GCCTAACCGG GGTTTACGAG 
CTAGCACTGC GCAGAGCATC CGAAGCAGGT AGTCCAGGTG TACAAAGGCG TCAACGTCGA 
GTGCTGGACA CCAGCTCTAC TTATGTGCGC GGCGAGGAGA ATCTTCATGG CTGGAGGCCA 
AGGGGTGACT CCCTGATCTT CGACCACCAG TGGGAGCTGG AGAAACTCAC CAGACTTGAA 
GAGGTTGGAC GCATGCGGCA CTTGCTTCTG CTGCGCGAAC GTCTGGGCAT GGACACCAAC 
CCAAATCCGA CCACCAAGAC CGAGAAGGAT GTATGCAATC TAGCTGCTCG GGCAGCCACA 
TCACCCGTAC ATATGGTCAT TCCACAATCG CCGCAGACTC CGGTCAAGGA CCCACAGCAA 
ATCATTCCAG AACGCGAATA CAACCAACGG GAGCAGGATC TCATGCTTAA ATGCTTAAAG 
TTGGTGCAGG GACGCTATAC TAAGAGCGAG GCCAACGATA CGCAAACTCA GTCGGATGTT 
TCGCCTAGCG ATGAGGGATG CGCCGACATG ACCGTCAGCT GCATCTCCAG CAATTCCATG 
GAAAACAACA AATTTGTAAT TCGACGCAGA TTATGTTCAC CGGATCGAGC TGATGCACCC 
AATGGCTGGG AGGCACCTGC TCCGGCTACT CAGCCGGCTC TTCCCCTCCG TCTCTATGTG 
CCGGAACTGG AGGAGATTCG CGTGAGTCCT GTGGTGGCCC GCAAGGGTCT GTTGAATGTC 
CTGGAGCACG GCGGTTCCGG CTGGAAGAAG CGCTGGGTGA TCGTCCGTCG CCCGTACGTG 
TTTATCTACC GCTCGGAGAA GGATCCCGTT GAACGGGCTG TCCTCAATCT GGCGACTGCG 
CATGTGGAGT GCAGCGAGGA CCAGGCGGCC ATGGTCAAGA TACCCAACAC ATTCAGTGTG 
GTGACCAAGC ATCGTGGCTA TCTGCTGCAA ACTCTCGGTG ACAAGGAAGT ACACGACTGG 
CTGTATGCTA TCAATCCATT GCTTGCTGGG CAGATAAAAT CCCGCCTGGC GCGACGGACT 
TTGGAGCCTG CTAGCCAGAC GGCTTCCCAG ATCCAGGCCA CCAATGCGGC GAATGCCAAT 
AGTGCGAGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097346.1
CDS	449..5503
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEGPDGKVVCEKRDANKDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMENNKFVIRRRLCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK

Target ORF information:

RefSeq Version	NM_001103876.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103876.3

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGGA CCCGATGGCA AAGTGGTGTG TGAGAAGCGC 
GATGCGAATA AGGATGAGCT CACCAAGTCA ACGGTGATCA AGTCGCCCAC TAAGTCACGT 
AATCGTAATG GATCCACCAC GGAGATGGCT GTGGATCAGC TGCAGGCCAG CGAGAAACTT 
ATTGCAGAGC TCAACGAGAC CTGGGAGGAG AAACTTAAGC GCACCGAGGA GATTCGCGTG 
CAGCGAGAGG CGGTCTTCGC CGAAATGGGT GTGGCTGTCA AGGAAGATGG AATAACAGTT 
GGCGTATTCT CACCGAAGAA GACTCCGCAT TTGGTCAACC TAAACGAGGA TCCCAATCTG 
TCTGAGTGTC TGCTTTACTA CATCAAGGAG GGTCTAACTC GGTTGGGTAC CCATGAAGCA 
AATGTGCCCC AGGACATTCA GCTCTCCGGA TCGCACATCC TCAAAGAGCA CTGCACCTTT 
GAGAACAAGA ACAGCACGGT TACCCTGCTG CCACACAAGG ATGCTATCAT CTATGTAAAT 
GGACGCAAGT TGGTTGAACC GGAGGTTCTT AAGACCGGTT CTCGCGTGAT CCTCGGAAAG 
AACCACGTGT TCCGCTTTAC CAATCCAGAA CAGGCACGCG AATTACGGGA TAAGATCGAG 
ACCGAAAATG AGGCTGAGAA CGAAGTGGAG AAGACAGACA CCCAGCAGGT GGACTGGAAT 
TTTGCCCAGT GCGAATTGCT CGAGAAGCAA GGCATTGATC TTAAAGCTGA AATGAAGAAG 
CGTTTAGACA ACTTGGAGGA ACAATACAAG CGGGAGAAAC TTCAGGCCGA TCAGCAATTC 
GAGGAGCAGC GCAAAACGTA CGAGGCTCGC ATCGATGCTT TGCAAAAACA GGTTGAGGAG 
CAATCCATGA CCATGTCCAT GTACAGCAGC TACTCGCCGG AGGATTTCCA CCAGGAGGAG 
GACGTCTACA CCAACCCCAT GTACGAATCC TGCTGGACAG CAAGAGAGGC TGGCTTGGCT 
GCCTGGGCTT TCCGCAAGTG GCGTTACCAC CAATTCACCT CTTTGCGAGA TGATCTCTGG 
GGCAATGCTA TATTCCTTAA GGAAGCGAAT GCCATTTCCG TTGAGTTAAA GAAGAAGGTA 
CAATTCCAAT TTACTCTCTT GACCGACACC TTGTACTCTC CTTTGCCGCC TGAGCTGGCA 
TCCACTGTGG CTCCTGTGCA TCAGGAGGAT GAGTTCGGAG CTCCACCTGT CTCTAAGACC 
TTGGTGGCCG TCGAAGTAAC CGATACTAAG AACGGAGCCA CTCACCACTG GTCTCTGGAG 
AAGTTACGGC AACGTTTGGA GCTGATGCGC GAGATGTACC ACAACGAAGC CGAAATGAGT 
CCCACTTCAC CGGACTATAA TGTGGAGAGC CTCACTGGTG GAGATCCATT CTACGATCGC 
TTCCCCTGGT TCCGCATGGT GGGTCGCTCC TTCATCTATC TGAGCAACCT GCTCTACCCA 
GTGCCATTGG TCCACAAGGT GGCCATTGTC AACGAACGCG GAGATGTGCG TGGCTACCTA 
AGGATTGCTG TGCAGCCCGT TCTGGATGAG GAGTCCATTG ATTTCAATAA TGGTGTCAAG 
CAGTCAGCTC GCTTGGTTTT CAATGAGGAT GATGCCAAAC CGAAATACCG AGCTCTCAAT 
GAAAAGGATG ATGTGCAGCG CTATATTGAC AATGGTGGTT TGGACAGCAA GCTGGAGGAA 
CTTGAGGATG TGGACTCTGG TCGTGGCATT GACTCTAACT CCGCATCCGA GTGCCACGAA 
AATTCGGAGG AGCCTGGCGA GCATCTGCAG GTGGGCAAGG AGTTCACTTT CCGGGTCACT 
GTGCTACAGG CCACTGGTAT TGGGGCTGAA TATGCCGATA TCTTCTGCCA GTTCAACTTT 
TTGCATCGTC ATGAGGAGGC TTTCTCCACC GAACCCGTCA AGAATTCGGC ATCGGGTGCT 
CCTCTAGGCT TCTACCATGT GCAGAACATA ACTGTACCCG TAACCAAATC CTTCATCGAG 
TATTTGAAGA CCCAACCCAT AATGTTCAAG ATATTTGGGC ACTACCAGAC GCACCCATTG 
CATAAGGATG CCAAACAGGA TTTTGTTTCT CGGCCACCAC CACGTCGCAT GTTGCCACCG 
AGCATACCGA TCAGTCAGCC AGTGCGTAGT CCCAAGTTTG GACCACTACC GTGTGCACCC 
ACGTCTACGG TTTTGGCTAA GCACGATGTT TTGGTTTGGT TTGAAATCTG TGAATTGGCT 
CCCAATGGAG AATATGTGCC ATCGGTGGTG GAGCACAGCG ATGATCTTCC CTGCCGCGGA 
CTGTTCCTCT TGCATCAGGG CATCCAGCGG CGCATTCGTA TTACTATCGT ACATGAGCCC 
ACAACCGAGG TGAAGTGGAA GGACATCAAT GAGTTGGTGG TTGGACGTAT CCGCAATACA 
CCGGAGTCAT CCGACGAACA GGACGAAGAC GCCTGTGTCT TATCTTTGGG CTTGTTCCCA 
GGCGAGGCTT TGGAGGTGCC CGGAGATGAT AGATCTTTCT ACCGTTTTGA GGCTGCCTGG 
GACTCGAGTC TGCACAACTC GGCACTGCTC AACCGCGTTT CTCAAGGCGG TGAGACCATC 
TACATCACCC TGAGCGCTTA CTTGGAGCTG GAGAATTGCG CCCGCCCGGC CATAATTACC 
AAGGACCTGA GCATGGTTAT CTATGGACGC GATGCCCGCA CCGGTCCGCG CTCCCTGAAG 
CACCTGTTTT CGGGACAGTA CCGCAATCCG GAGGCCAATC GCCTAACCGG GGTTTACGAG 
CTAGCACTGC GCAGAGCATC CGAAGCAGGT AGTCCAGGTG TACAAAGGCG TCAACGTCGA 
GTGCTGGACA CCAGCTCTAC TTATGTGCGC GGCGAGGAGA ATCTTCATGG CTGGAGGCCA 
AGGGGTGACT CCCTGATCTT CGACCACCAG TGGGAGCTGG AGAAACTCAC CAGACTTGAA 
GAGGTTGGAC GCATGCGGCA CTTGCTTCTG CTGCGCGAAC GTCTGGGCAT GGACACCAAC 
CCAAATCCGA CCACCAAGAC CGAGAAGGAT GTATGCAATC TAGCTGCTCG GGCAGCCACA 
TCACCCGTAC ATATGGTCAT TCCACAATCG CCGCAGACTC CGGTCAAGGA CCCACAGCAA 
ATCATTCCAG AACGCGAATA CAACCAACGG GAGCAGGATC TCATGCTTAA ATGCTTAAAG 
TTGGTGCAGG GACGCTATAC TAAGAGCGAG GCCAACGATA CGCAAACTCA GTCGGATGTT 
TCGCCTAGCG ATGAGGGATG CGCCGACATG ACCGTCAGCT GCATCTCCAG CAATTCCATG 
GAAAACAACA AATTTGTAAT TCGACGCAGA TTATGTTCAC CGGATCGAGC TGATGCACCC 
AATGGCTGGG AGGCACCTGC TCCGGCTACT CAGCCGGCTC TTCCCCTCCG TCTCTATGTG 
CCGGAACTGG AGGAGATTCG CGTGAGTCCT GTGGTGGCCC GCAAGGGTCT GTTGAATGTC 
CTGGAGCACG GCGGTTCCGG CTGGAAGAAG CGCTGGGTGA TCGTCCGTCG CCCGTACGTG 
TTTATCTACC GCTCGGAGAA GGATCCCGTT GAACGGGCTG TCCTCAATCT GGCGACTGCG 
CATGTGGAGT GCAGCGAGGA CCAGGCGGCC ATGGTCAAGA TACCCAACAC ATTCAGTGTG 
GTGACCAAGC ATCGTGGCTA TCTGCTGCAA ACTCTCGGTG ACAAGGAAGT ACACGACTGG 
CTGTATGCTA TCAATCCATT GCTTGCTGGG CAGATAAAAT CCCGCCTGGC GCGACGGACT 
TTGGAGCCTG CTAGCCAGAC GGCTTCCCAG ATCCAGGCCA CCAATGCGGC GAATGCCAAT 
AGTGCGAGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-104 ( NM_001274104.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261033.1 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant H, mRNA.

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CloneID	OFa12366
Clone ID Related Accession (Same CDS sequence)	NM_001274104.2
Accession Version	NM_001274104.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274104.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA ATTATGTTCA CCGGATCGAG CTGATGCACC CAATGGCTGG 
GAGGCACCTG CTCCGGCTAC TCAGCCGGCT CTTCCCCTCC GTCTCTATGT GCCGGAACTG 
GAGGAGATTC GCGTGAGTCC TGTGGTGGCC CGCAAGGGTC TGTTGAATGT CCTGGAGCAC 
GGCGGTTCCG GCTGGAAGAA GCGCTGGGTG ATCGTCCGTC GCCCGTACGT GTTTATCTAC 
CGCTCGGAGA AGGATCCCGT TGAACGGGCT GTCCTCAATC TGGCGACTGC GCATGTGGAG 
TGCAGCGAGG ACCAGGCGGC CATGGTCAAG ATACCCAACA CATTCAGTGT GGTGACCAAG 
CATCGTGGCT ATCTGCTGCA AACTCTCGGT GACAAGGAAG TACACGACTG GCTGTATGCT 
ATCAATCCAT TGCTTGCTGG GCAGATAAAA TCCCGCCTGG CGCGACGGAC TTTGGAGCCT 
GCTAGCCAGA CGGCTTCCCA GATCCAGGCC ACCAATGCGG CGAATGCCAA TAGTGCGAGC 
AAATGAGATA CGATTACGAT GGAGTCCGTT TTGGTTTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261033.1
CDS	291..5312
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEEDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMELCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK*DTITMESVLVY

Target ORF information:

RefSeq Version	NM_001274104.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274104.2

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGAG GATGAGCTCA CCAAGTCAAC GGTGATCAAG 
TCGCCCACTA AGTCACGTAA TCGTAATGGA TCCACCACGG AGATGGCTGT GGATCAGCTG 
CAGGCCAGCG AGAAACTTAT TGCAGAGCTC AACGAGACCT GGGAGGAGAA ACTTAAGCGC 
ACCGAGGAGA TTCGCGTGCA GCGAGAGGCG GTCTTCGCCG AAATGGGTGT GGCTGTCAAG 
GAAGATGGAA TAACAGTTGG CGTATTCTCA CCGAAGAAGA CTCCGCATTT GGTCAACCTA 
AACGAGGATC CCAATCTGTC TGAGTGTCTG CTTTACTACA TCAAGGAGGG TCTAACTCGG 
TTGGGTACCC ATGAAGCAAA TGTGCCCCAG GACATTCAGC TCTCCGGATC GCACATCCTC 
AAAGAGCACT GCACCTTTGA GAACAAGAAC AGCACGGTTA CCCTGCTGCC ACACAAGGAT 
GCTATCATCT ATGTAAATGG ACGCAAGTTG GTTGAACCGG AGGTTCTTAA GACCGGTTCT 
CGCGTGATCC TCGGAAAGAA CCACGTGTTC CGCTTTACCA ATCCAGAACA GGCACGCGAA 
TTACGGGATA AGATCGAGAC CGAAAATGAG GCTGAGAACG AAGTGGAGAA GACAGACACC 
CAGCAGGTGG ACTGGAATTT TGCCCAGTGC GAATTGCTCG AGAAGCAAGG CATTGATCTT 
AAAGCTGAAA TGAAGAAGCG TTTAGACAAC TTGGAGGAAC AATACAAGCG GGAGAAACTT 
CAGGCCGATC AGCAATTCGA GGAGCAGCGC AAAACGTACG AGGCTCGCAT CGATGCTTTG 
CAAAAACAGG TTGAGGAGCA ATCCATGACC ATGTCCATGT ACAGCAGCTA CTCGCCGGAG 
GATTTCCACC AGGAGGAGGA CGTCTACACC AACCCCATGT ACGAATCCTG CTGGACAGCA 
AGAGAGGCTG GCTTGGCTGC CTGGGCTTTC CGCAAGTGGC GTTACCACCA ATTCACCTCT 
TTGCGAGATG ATCTCTGGGG CAATGCTATA TTCCTTAAGG AAGCGAATGC CATTTCCGTT 
GAGTTAAAGA AGAAGGTACA ATTCCAATTT ACTCTCTTGA CCGACACCTT GTACTCTCCT 
TTGCCGCCTG AGCTGGCATC CACTGTGGCT CCTGTGCATC AGGAGGATGA GTTCGGAGCT 
CCACCTGTCT CTAAGACCTT GGTGGCCGTC GAAGTAACCG ATACTAAGAA CGGAGCCACT 
CACCACTGGT CTCTGGAGAA GTTACGGCAA CGTTTGGAGC TGATGCGCGA GATGTACCAC 
AACGAAGCCG AAATGAGTCC CACTTCACCG GACTATAATG TGGAGAGCCT CACTGGTGGA 
GATCCATTCT ACGATCGCTT CCCCTGGTTC CGCATGGTGG GTCGCTCCTT CATCTATCTG 
AGCAACCTGC TCTACCCAGT GCCATTGGTC CACAAGGTGG CCATTGTCAA CGAACGCGGA 
GATGTGCGTG GCTACCTAAG GATTGCTGTG CAGCCCGTTC TGGATGAGGA GTCCATTGAT 
TTCAATAATG GTGTCAAGCA GTCAGCTCGC TTGGTTTTCA ATGAGGATGA TGCCAAACCG 
AAATACCGAG CTCTCAATGA AAAGGATGAT GTGCAGCGCT ATATTGACAA TGGTGGTTTG 
GACAGCAAGC TGGAGGAACT TGAGGATGTG GACTCTGGTC GTGGCATTGA CTCTAACTCC 
GCATCCGAGT GCCACGAAAA TTCGGAGGAG CCTGGCGAGC ATCTGCAGGT GGGCAAGGAG 
TTCACTTTCC GGGTCACTGT GCTACAGGCC ACTGGTATTG GGGCTGAATA TGCCGATATC 
TTCTGCCAGT TCAACTTTTT GCATCGTCAT GAGGAGGCTT TCTCCACCGA ACCCGTCAAG 
AATTCGGCAT CGGGTGCTCC TCTAGGCTTC TACCATGTGC AGAACATAAC TGTACCCGTA 
ACCAAATCCT TCATCGAGTA TTTGAAGACC CAACCCATAA TGTTCAAGAT ATTTGGGCAC 
TACCAGACGC ACCCATTGCA TAAGGATGCC AAACAGGATT TTGTTTCTCG GCCACCACCA 
CGTCGCATGT TGCCACCGAG CATACCGATC AGTCAGCCAG TGCGTAGTCC CAAGTTTGGA 
CCACTACCGT GTGCACCCAC GTCTACGGTT TTGGCTAAGC ACGATGTTTT GGTTTGGTTT 
GAAATCTGTG AATTGGCTCC CAATGGAGAA TATGTGCCAT CGGTGGTGGA GCACAGCGAT 
GATCTTCCCT GCCGCGGACT GTTCCTCTTG CATCAGGGCA TCCAGCGGCG CATTCGTATT 
ACTATCGTAC ATGAGCCCAC AACCGAGGTG AAGTGGAAGG ACATCAATGA GTTGGTGGTT 
GGACGTATCC GCAATACACC GGAGTCATCC GACGAACAGG ACGAAGACGC CTGTGTCTTA 
TCTTTGGGCT TGTTCCCAGG CGAGGCTTTG GAGGTGCCCG GAGATGATAG ATCTTTCTAC 
CGTTTTGAGG CTGCCTGGGA CTCGAGTCTG CACAACTCGG CACTGCTCAA CCGCGTTTCT 
CAAGGCGGTG AGACCATCTA CATCACCCTG AGCGCTTACT TGGAGCTGGA GAATTGCGCC 
CGCCCGGCCA TAATTACCAA GGACCTGAGC ATGGTTATCT ATGGACGCGA TGCCCGCACC 
GGTCCGCGCT CCCTGAAGCA CCTGTTTTCG GGACAGTACC GCAATCCGGA GGCCAATCGC 
CTAACCGGGG TTTACGAGCT AGCACTGCGC AGAGCATCCG AAGCAGGTAG TCCAGGTGTA 
CAAAGGCGTC AACGTCGAGT GCTGGACACC AGCTCTACTT ATGTGCGCGG CGAGGAGAAT 
CTTCATGGCT GGAGGCCAAG GGGTGACTCC CTGATCTTCG ACCACCAGTG GGAGCTGGAG 
AAACTCACCA GACTTGAAGA GGTTGGACGC ATGCGGCACT TGCTTCTGCT GCGCGAACGT 
CTGGGCATGG ACACCAACCC AAATCCGACC ACCAAGACCG AGAAGGATGT ATGCAATCTA 
GCTGCTCGGG CAGCCACATC ACCCGTACAT ATGGTCATTC CACAATCGCC GCAGACTCCG 
GTCAAGGACC CACAGCAAAT CATTCCAGAA CGCGAATACA ACCAACGGGA GCAGGATCTC 
ATGCTTAAAT GCTTAAAGTT GGTGCAGGGA CGCTATACTA AGAGCGAGGC CAACGATACG 
CAAACTCAGT CGGATGTTTC GCCTAGCGAT GAGGGATGCG CCGACATGAC CGTCAGCTGC 
ATCTCCAGCA ATTCCATGGA ATTATGTTCA CCGGATCGAG CTGATGCACC CAATGGCTGG 
GAGGCACCTG CTCCGGCTAC TCAGCCGGCT CTTCCCCTCC GTCTCTATGT GCCGGAACTG 
GAGGAGATTC GCGTGAGTCC TGTGGTGGCC CGCAAGGGTC TGTTGAATGT CCTGGAGCAC 
GGCGGTTCCG GCTGGAAGAA GCGCTGGGTG ATCGTCCGTC GCCCGTACGT GTTTATCTAC 
CGCTCGGAGA AGGATCCCGT TGAACGGGCT GTCCTCAATC TGGCGACTGC GCATGTGGAG 
TGCAGCGAGG ACCAGGCGGC CATGGTCAAG ATACCCAACA CATTCAGTGT GGTGACCAAG 
CATCGTGGCT ATCTGCTGCA AACTCTCGGT GACAAGGAAG TACACGACTG GCTGTATGCT 
ATCAATCCAT TGCTTGCTGG GCAGATAAAA TCCCGCCTGG CGCGACGGAC TTTGGAGCCT 
GCTAGCCAGA CGGCTTCCCA GATCCAGGCC ACCAATGCGG CGAATGCCAA TAGTGCGAGC 
AAATGAGATA CGATTACGAT GGAGTCCGTT TTGGTTTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-104 ( NM_001259445.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246374.1 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant G, mRNA.

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CloneID	OFa12369
Clone ID Related Accession (Same CDS sequence)	NM_001259445.2
Accession Version	NM_001259445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259445.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGGA CCCGATGGCA AAGTGGTGTG TGAGAAGCGC 
GATGCGAATA AGGATGAGCT CACCAAGTCA ACGGTGATCA AGTCGCCCAC TAAGTCACGT 
AATCGTAATG GATCCACCAC GGAGATGGCT GTGGATCAGC TGCAGGCCAG CGAGAAACTT 
ATTGCAGAGC TCAACGAGAC CTGGGAGGAG AAACTTAAGC GCACCGAGGA GATTCGCGTG 
CAGCGAGAGG CGGTCTTCGC CGAAATGGGT GTGGCTGTCA AGGAAGATGG AATAACAGTT 
GGCGTATTCT CACCGAAGAA GACTCCGCAT TTGGTCAACC TAAACGAGGA TCCCAATCTG 
TCTGAGTGTC TGCTTTACTA CATCAAGGAG GGTCTAACTC GGTTGGGTAC CCATGAAGCA 
AATGTGCCCC AGGACATTCA GCTCTCCGGA TCGCACATCC TCAAAGAGCA CTGCACCTTT 
GAGAACAAGA ACAGCACGGT TACCCTGCTG CCACACAAGG ATGCTATCAT CTATGTAAAT 
GGACGCAAGT TGGTTGAACC GGAGGTTCTT AAGACCGGTT CTCGCGTGAT CCTCGGAAAG 
AACCACGTGT TCCGCTTTAC CAATCCAGAA CAGGCACGCG AATTACGGGA TAAGATCGAG 
ACCGAAAATG AGGCTGAGAA CGAAGTGGAG AAGACAGACA CCCAGCAGGT GGACTGGAAT 
TTTGCCCAGT GCGAATTGCT CGAGAAGCAA GGCATTGATC TTAAAGCTGA AATGAAGAAG 
CGTTTAGACA ACTTGGAGGA ACAATACAAG CGGGAGAAAC TTCAGGCCGA TCAGCAATTC 
GAGGAGCAGC GCAAAACGTA CGAGGCTCGC ATCGATGCTT TGCAAAAACA GGTTGAGGAG 
CAATCCATGA CCATGTCCAT GTACAGCAGC TACTCGCCGG AGGATTTCCA CCAGGAGGAG 
GACGTCTACA CCAACCCCAT GTACGAATCC TGCTGGACAG CAAGAGAGGC TGGCTTGGCT 
GCCTGGGCTT TCCGCAAGTG GCGTTACCAC CAATTCACCT CTTTGCGAGA TGATCTCTGG 
GGCAATGCTA TATTCCTTAA GGAAGCGAAT GCCATTTCCG TTGAGTTAAA GAAGAAGGTA 
CAATTCCAAT TTACTCTCTT GACCGACACC TTGTACTCTC CTTTGCCGCC TGAGCTGGCA 
TCCACTGTGG CTCCTGTGCA TCAGGAGGAT GAGTTCGGAG CTCCACCTGT CTCTAAGACC 
TTGGTGGCCG TCGAAGTAAC CGATACTAAG AACGGAGCCA CTCACCACTG GTCTCTGGAG 
AAGTTACGGC AACGTTTGGA GCTGATGCGC GAGATGTACC ACAACGAAGC CGAAATGAGT 
CCCACTTCAC CGGACTATAA TGTGGAGAGC CTCACTGGTG GAGATCCATT CTACGATCGC 
TTCCCCTGGT TCCGCATGGT GGGTCGCTCC TTCATCTATC TGAGCAACCT GCTCTACCCA 
GTGCCATTGG TCCACAAGGT GGCCATTGTC AACGAACGCG GAGATGTGCG TGGCTACCTA 
AGGATTGCTG TGCAGCCCGT TCTGGATGAG GAGTCCATTG ATTTCAATAA TGGTGTCAAG 
CAGTCAGCTC GCTTGGTTTT CAATGAGGAT GATGCCAAAC CGAAATACCG AGCTCTCAAT 
GAAAAGGATG ATGTGCAGCG CTATATTGAC AATGGTGGTT TGGACAGCAA GCTGGAGGAA 
CTTGAGGATG TGGACTCTGG TCGTGGCATT GACTCTAACT CCGCATCCGA GTGCCACGAA 
AATTCGGAGG AGCCTGGCGA GCATCTGCAG GTGGGCAAGG AGTTCACTTT CCGGGTCACT 
GTGCTACAGG CCACTGGTAT TGGGGCTGAA TATGCCGATA TCTTCTGCCA GTTCAACTTT 
TTGCATCGTC ATGAGGAGGC TTTCTCCACC GAACCCGTCA AGAATTCGGC ATCGGGTGCT 
CCTCTAGGCT TCTACCATGT GCAGAACATA ACTGTACCCG TAACCAAATC CTTCATCGAG 
TATTTGAAGA CCCAACCCAT AATGTTCAAG ATATTTGGGC ACTACCAGAC GCACCCATTG 
CATAAGGATG CCAAACAGGA TTTTGTTTCT CGGCCACCAC CACGTCGCAT GTTGCCACCG 
AGCATACCGA TCAGTCAGCC AGTGCGTAGT CCCAAGTTTG GACCACTACC GTGTGCACCC 
ACGTCTACGG TTTTGGCTAA GCACGATGTT TTGGTTTGGT TTGAAATCTG TGAATTGGCT 
CCCAATGGAG AATATGTGCC ATCGGTGGTG GAGCACAGCG ATGATCTTCC CTGCCGCGGA 
CTGTTCCTCT TGCATCAGGG CATCCAGCGG CGCATTCGTA TTACTATCGT ACATGAGCCC 
ACAACCGAGG TGAAGTGGAA GGACATCAAT GAGTTGGTGG TTGGACGTAT CCGCAATACA 
CCGGAGTCAT CCGACGAACA GGACGAAGAC GCCTGTGTCT TATCTTTGGG CTTGTTCCCA 
GGCGAGGCTT TGGAGGTGCC CGGAGATGAT AGATCTTTCT ACCGTTTTGA GGCTGCCTGG 
GACTCGAGTC TGCACAACTC GGCACTGCTC AACCGCGTTT CTCAAGGCGG TGAGACCATC 
TACATCACCC TGAGCGCTTA CTTGGAGCTG GAGAATTGCG CCCGCCCGGC CATAATTACC 
AAGGACCTGA GCATGGTTAT CTATGGACGC GATGCCCGCA CCGGTCCGCG CTCCCTGAAG 
CACCTGTTTT CGGGACAGTA CCGCAATCCG GAGGCCAATC GCCTAACCGG GGTTTACGAG 
CTAGCACTGC GCAGAGCATC CGAAGCAGGT GTACAAAGGC GTCAACGTCG AGTGCTGGAC 
ACCAGCTCTA CTTATGTGCG CGGCGAGGAG AATCTTCATG GCTGGAGGCC AAGGGGTGAC 
TCCCTGATCT TCGACCACCA GTGGGAGCTG GAGAAACTCA CCAGACTTGA AGAGGTTGGA 
CGCATGCGGC ACTTGCTTCT GCTGCGCGAA CGTCTGGGCA TGGACACCAA CCCAAATCCG 
ACCACCAAGA CCGAGAAGGA TGTATGCAAT CTAGCTGCTC GGGCAGCCAC ATCACCCGTA 
CATATGGTCA TTCCACAATC GCCGCAGACT CCGGTCAAGG ACCCACAGCA AATCATTCCA 
GAACGCGAAT ACAACCAACG GGAGCAGGAT CTCATGCTTA AATGCTTAAA GTTGGTGCAG 
GGACGCTATA CTAAGAGCGA GGCCAACGAT ACGCAAACTC AGTCGGATGT TTCGCCTAGC 
GATGAGGGAT GCGCCGACAT GACCGTCAGC TGCATCTCCA GCAATTCCAT GGAAAACAAC 
AAATTTGTAA TTCGACGCAG ATTATGTTCA CCGGATCGAG CTGATGCACC CAATGGCTGG 
GAGGCACCTG CTCCGGCTAC TCAGCCGGCT CTTCCCCTCC GTCTCTATGT GCCGGAACTG 
GAGGAGATTC GCGTGAGTCC TGTGGTGGCC CGCAAGGGTC TGTTGAATGT CCTGGAGCAC 
GGCGGTTCCG GCTGGAAGAA GCGCTGGGTG ATCGTCCGTC GCCCGTACGT GTTTATCTAC 
CGCTCGGAGA AGGATCCCGT TGAACGGGCT GTCCTCAATC TGGCGACTGC GCATGTGGAG 
TGCAGCGAGG ACCAGGCGGC CATGGTCAAG ATACCCAACA CATTCAGTGT GGTGACCAAG 
CATCGTGGCT ATCTGCTGCA AACTCTCGGT GACAAGGAAG TACACGACTG GCTGTATGCT 
ATCAATCCAT TGCTTGCTGG GCAGATAAAA TCCCGCCTGG CGCGACGGAC TTTGGAGCCT 
GCTAGCCAGA CGGCTTCCCA GATCCAGGCC ACCAATGCGG CGAATGCCAA TAGTGCGAGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246374.1
CDS	449..5494
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEGPDGKVVCEKRDANKDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMENNKFVIRRRLCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK

Target ORF information:

RefSeq Version	NM_001259445.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259445.2

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGGA CCCGATGGCA AAGTGGTGTG TGAGAAGCGC 
GATGCGAATA AGGATGAGCT CACCAAGTCA ACGGTGATCA AGTCGCCCAC TAAGTCACGT 
AATCGTAATG GATCCACCAC GGAGATGGCT GTGGATCAGC TGCAGGCCAG CGAGAAACTT 
ATTGCAGAGC TCAACGAGAC CTGGGAGGAG AAACTTAAGC GCACCGAGGA GATTCGCGTG 
CAGCGAGAGG CGGTCTTCGC CGAAATGGGT GTGGCTGTCA AGGAAGATGG AATAACAGTT 
GGCGTATTCT CACCGAAGAA GACTCCGCAT TTGGTCAACC TAAACGAGGA TCCCAATCTG 
TCTGAGTGTC TGCTTTACTA CATCAAGGAG GGTCTAACTC GGTTGGGTAC CCATGAAGCA 
AATGTGCCCC AGGACATTCA GCTCTCCGGA TCGCACATCC TCAAAGAGCA CTGCACCTTT 
GAGAACAAGA ACAGCACGGT TACCCTGCTG CCACACAAGG ATGCTATCAT CTATGTAAAT 
GGACGCAAGT TGGTTGAACC GGAGGTTCTT AAGACCGGTT CTCGCGTGAT CCTCGGAAAG 
AACCACGTGT TCCGCTTTAC CAATCCAGAA CAGGCACGCG AATTACGGGA TAAGATCGAG 
ACCGAAAATG AGGCTGAGAA CGAAGTGGAG AAGACAGACA CCCAGCAGGT GGACTGGAAT 
TTTGCCCAGT GCGAATTGCT CGAGAAGCAA GGCATTGATC TTAAAGCTGA AATGAAGAAG 
CGTTTAGACA ACTTGGAGGA ACAATACAAG CGGGAGAAAC TTCAGGCCGA TCAGCAATTC 
GAGGAGCAGC GCAAAACGTA CGAGGCTCGC ATCGATGCTT TGCAAAAACA GGTTGAGGAG 
CAATCCATGA CCATGTCCAT GTACAGCAGC TACTCGCCGG AGGATTTCCA CCAGGAGGAG 
GACGTCTACA CCAACCCCAT GTACGAATCC TGCTGGACAG CAAGAGAGGC TGGCTTGGCT 
GCCTGGGCTT TCCGCAAGTG GCGTTACCAC CAATTCACCT CTTTGCGAGA TGATCTCTGG 
GGCAATGCTA TATTCCTTAA GGAAGCGAAT GCCATTTCCG TTGAGTTAAA GAAGAAGGTA 
CAATTCCAAT TTACTCTCTT GACCGACACC TTGTACTCTC CTTTGCCGCC TGAGCTGGCA 
TCCACTGTGG CTCCTGTGCA TCAGGAGGAT GAGTTCGGAG CTCCACCTGT CTCTAAGACC 
TTGGTGGCCG TCGAAGTAAC CGATACTAAG AACGGAGCCA CTCACCACTG GTCTCTGGAG 
AAGTTACGGC AACGTTTGGA GCTGATGCGC GAGATGTACC ACAACGAAGC CGAAATGAGT 
CCCACTTCAC CGGACTATAA TGTGGAGAGC CTCACTGGTG GAGATCCATT CTACGATCGC 
TTCCCCTGGT TCCGCATGGT GGGTCGCTCC TTCATCTATC TGAGCAACCT GCTCTACCCA 
GTGCCATTGG TCCACAAGGT GGCCATTGTC AACGAACGCG GAGATGTGCG TGGCTACCTA 
AGGATTGCTG TGCAGCCCGT TCTGGATGAG GAGTCCATTG ATTTCAATAA TGGTGTCAAG 
CAGTCAGCTC GCTTGGTTTT CAATGAGGAT GATGCCAAAC CGAAATACCG AGCTCTCAAT 
GAAAAGGATG ATGTGCAGCG CTATATTGAC AATGGTGGTT TGGACAGCAA GCTGGAGGAA 
CTTGAGGATG TGGACTCTGG TCGTGGCATT GACTCTAACT CCGCATCCGA GTGCCACGAA 
AATTCGGAGG AGCCTGGCGA GCATCTGCAG GTGGGCAAGG AGTTCACTTT CCGGGTCACT 
GTGCTACAGG CCACTGGTAT TGGGGCTGAA TATGCCGATA TCTTCTGCCA GTTCAACTTT 
TTGCATCGTC ATGAGGAGGC TTTCTCCACC GAACCCGTCA AGAATTCGGC ATCGGGTGCT 
CCTCTAGGCT TCTACCATGT GCAGAACATA ACTGTACCCG TAACCAAATC CTTCATCGAG 
TATTTGAAGA CCCAACCCAT AATGTTCAAG ATATTTGGGC ACTACCAGAC GCACCCATTG 
CATAAGGATG CCAAACAGGA TTTTGTTTCT CGGCCACCAC CACGTCGCAT GTTGCCACCG 
AGCATACCGA TCAGTCAGCC AGTGCGTAGT CCCAAGTTTG GACCACTACC GTGTGCACCC 
ACGTCTACGG TTTTGGCTAA GCACGATGTT TTGGTTTGGT TTGAAATCTG TGAATTGGCT 
CCCAATGGAG AATATGTGCC ATCGGTGGTG GAGCACAGCG ATGATCTTCC CTGCCGCGGA 
CTGTTCCTCT TGCATCAGGG CATCCAGCGG CGCATTCGTA TTACTATCGT ACATGAGCCC 
ACAACCGAGG TGAAGTGGAA GGACATCAAT GAGTTGGTGG TTGGACGTAT CCGCAATACA 
CCGGAGTCAT CCGACGAACA GGACGAAGAC GCCTGTGTCT TATCTTTGGG CTTGTTCCCA 
GGCGAGGCTT TGGAGGTGCC CGGAGATGAT AGATCTTTCT ACCGTTTTGA GGCTGCCTGG 
GACTCGAGTC TGCACAACTC GGCACTGCTC AACCGCGTTT CTCAAGGCGG TGAGACCATC 
TACATCACCC TGAGCGCTTA CTTGGAGCTG GAGAATTGCG CCCGCCCGGC CATAATTACC 
AAGGACCTGA GCATGGTTAT CTATGGACGC GATGCCCGCA CCGGTCCGCG CTCCCTGAAG 
CACCTGTTTT CGGGACAGTA CCGCAATCCG GAGGCCAATC GCCTAACCGG GGTTTACGAG 
CTAGCACTGC GCAGAGCATC CGAAGCAGGT GTACAAAGGC GTCAACGTCG AGTGCTGGAC 
ACCAGCTCTA CTTATGTGCG CGGCGAGGAG AATCTTCATG GCTGGAGGCC AAGGGGTGAC 
TCCCTGATCT TCGACCACCA GTGGGAGCTG GAGAAACTCA CCAGACTTGA AGAGGTTGGA 
CGCATGCGGC ACTTGCTTCT GCTGCGCGAA CGTCTGGGCA TGGACACCAA CCCAAATCCG 
ACCACCAAGA CCGAGAAGGA TGTATGCAAT CTAGCTGCTC GGGCAGCCAC ATCACCCGTA 
CATATGGTCA TTCCACAATC GCCGCAGACT CCGGTCAAGG ACCCACAGCA AATCATTCCA 
GAACGCGAAT ACAACCAACG GGAGCAGGAT CTCATGCTTA AATGCTTAAA GTTGGTGCAG 
GGACGCTATA CTAAGAGCGA GGCCAACGAT ACGCAAACTC AGTCGGATGT TTCGCCTAGC 
GATGAGGGAT GCGCCGACAT GACCGTCAGC TGCATCTCCA GCAATTCCAT GGAAAACAAC 
AAATTTGTAA TTCGACGCAG ATTATGTTCA CCGGATCGAG CTGATGCACC CAATGGCTGG 
GAGGCACCTG CTCCGGCTAC TCAGCCGGCT CTTCCCCTCC GTCTCTATGT GCCGGAACTG 
GAGGAGATTC GCGTGAGTCC TGTGGTGGCC CGCAAGGGTC TGTTGAATGT CCTGGAGCAC 
GGCGGTTCCG GCTGGAAGAA GCGCTGGGTG ATCGTCCGTC GCCCGTACGT GTTTATCTAC 
CGCTCGGAGA AGGATCCCGT TGAACGGGCT GTCCTCAATC TGGCGACTGC GCATGTGGAG 
TGCAGCGAGG ACCAGGCGGC CATGGTCAAG ATACCCAACA CATTCAGTGT GGTGACCAAG 
CATCGTGGCT ATCTGCTGCA AACTCTCGGT GACAAGGAAG TACACGACTG GCTGTATGCT 
ATCAATCCAT TGCTTGCTGG GCAGATAAAA TCCCGCCTGG CGCGACGGAC TTTGGAGCCT 
GCTAGCCAGA CGGCTTCCCA GATCCAGGCC ACCAATGCGG CGAATGCCAA TAGTGCGAGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-104 ( NM_001259444.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246373.1 Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant F, mRNA.

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CloneID	OFa12371
Clone ID Related Accession (Same CDS sequence)	NM_001259444.2
Accession Version	NM_001259444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated-104, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259444.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGGA CCCGATGGCA AAGTGGTGTG TGAGAAGCGC 
GATGCGAATA AGGATGAGCT CACCAAGTCA ACGGTGATCA AGTCGCCCAC TAAGTCACGT 
AATCGTAATG GATCCACCAC GGAGATGGCT GTGGATCAGC TGCAGGCCAG CGAGAAACTT 
ATTGCAGAGC TCAACGAGAC CTGGGAGGAG AAACTTAAGC GCACCGAGGA GATTCGCGTG 
CAGCGAGAGG CGGTCTTCGC CGAAATGGGT GTGGCTGTCA AGGAAGATGG AATAACAGTT 
GGCGTATTCT CACCGAAGAA GACTCCGCAT TTGGTCAACC TAAACGAGGA TCCCAATCTG 
TCTGAGTGTC TGCTTTACTA CATCAAGGAG GGTCTAACTC GGTTGGGTAC CCATGAAGCA 
AATGTGCCCC AGGACATTCA GCTCTCCGGA TCGCACATCC TCAAAGAGCA CTGCACCTTT 
GAGAACAAGA ACAGCACGGT TACCCTGCTG CCACACAAGG ATGCTATCAT CTATGTAAAT 
GGACGCAAGT TGGTTGAACC GGAGGTTCTT AAGACCGGTT CTCGCGTGAT CCTCGGAAAG 
AACCACGTGT TCCGCTTTAC CAATCCAGAA CAGGCACGCG AATTACGGGA TAAGATCGAG 
ACCGAAAATG AGGCTGAGAA CGAAGTGGAG AAGACAGACA CCCAGCAGGT GGACTGGAAT 
TTTGCCCAGT GCGAATTGCT CGAGAAGCAA GGCATTGATC TTAAAGCTGA AATGAAGAAG 
CGTTTAGACA ACTTGGAGGA ACAATACAAG CGGGAGAAAC TTCAGGCCGA TCAGCAATTC 
GAGGAGCAGC GCAAAACGTA CGAGGCTCGC ATCGATGCTT TGCAAAAACA GGTTGAGGAG 
CAATCCATGA CCATGTCCAT GTACAGCAGC TACTCGCCGG AGGATTTCCA CCAGGAGGAG 
GACGTCTACA CCAACCCCAT GTACGAATCC TGCTGGACAG CAAGAGAGGC TGGCTTGGCT 
GCCTGGGCTT TCCGCAAGTG GCGTTACCAC CAATTCACCT CTTTGCGAGA TGATCTCTGG 
GGCAATGCTA TATTCCTTAA GGAAGCGAAT GCCATTTCCG TTGAGTTAAA GAAGAAGGTA 
CAATTCCAAT TTACTCTCTT GACCGACACC TTGTACTCTC CTTTGCCGCC TGAGCTGGCA 
TCCACTGTGG CTCCTGTGCA TCAGGAGGAT GAGTTCGGAG CTCCACCTGT CTCTAAGACC 
TTGGTGGCCG TCGAAGTAAC CGATACTAAG AACGGAGCCA CTCACCACTG GTCTCTGGAG 
AAGTTACGGC AACGTTTGGA GCTGATGCGC GAGATGTACC ACAACGAAGC CGAAATGAGT 
CCCACTTCAC CGGACTATAA TGTGGAGAGC CTCACTGGTG GAGATCCATT CTACGATCGC 
TTCCCCTGGT TCCGCATGGT GGGTCGCTCC TTCATCTATC TGAGCAACCT GCTCTACCCA 
GTGCCATTGG TCCACAAGGT GGCCATTGTC AACGAACGCG GAGATGTGCG TGGCTACCTA 
AGGATTGCTG TGCAGCCCGT TCTGGATGAG GAGTCCATTG ATTTCAATAA TGGTGTCAAG 
CAGTCAGCTC GCTTGGTTTT CAATGAGGAT GATGCCAAAC CGAAATACCG AGCTCTCAAT 
GAAAAGGATG ATGTGCAGCG CTATATTGAC AATGGTGGTT TGGACAGCAA GCTGGAGGAA 
CTTGAGGATG TGGACTCTGG TCGTGGCATT GACTCTAACT CCGCATCCGA GTGCCACGAA 
AATTCGGAGG AGCCTGGCGA GCATCTGCAG GTGGGCAAGG AGTTCACTTT CCGGGTCACT 
GTGCTACAGG CCACTGGTAT TGGGGCTGAA TATGCCGATA TCTTCTGCCA GTTCAACTTT 
TTGCATCGTC ATGAGGAGGC TTTCTCCACC GAACCCGTCA AGAATTCGGC ATCGGGTGCT 
CCTCTAGGCT TCTACCATGT GCAGAACATA ACTGTACCCG TAACCAAATC CTTCATCGAG 
TATTTGAAGA CCCAACCCAT AATGTTCAAG ATATTTGGGC ACTACCAGAC GCACCCATTG 
CATAAGGATG CCAAACAGGA TTTTGTTTCT CGGCCACCAC CACGTCGCAT GTTGCCACCG 
AGCATACCGA TCAGTCAGCC AGTGCGTAGT CCCAAGTTTG GACCACTACC GTGTGCACCC 
ACGTCTACGG TTTTGGCTAA GCACGATGTT TTGGTTTGGT TTGAAATCTG TGAATTGGCT 
CCCAATGGAG AATATGTGCC ATCGGTGGTG GAGCACAGCG ATGATCTTCC CTGCCGCGGA 
CTGTTCCTCT TGCATCAGGG CATCCAGCGG CGCATTCGTA TTACTATCGT ACATGAGCCC 
ACAACCGAGG TGAAGTGGAA GGACATCAAT GAGTTGGTGG TTGGACGTAT CCGCAATACA 
CCGGAGTCAT CCGACGAACA GGACGAAGAC GCCTGTGTCT TATCTTTGGG CTTGTTCCCA 
GGCGAGGCTT TGGAGGTGCC CGGAGATGAT AGATCTTTCT ACCGTTTTGA GGCTGCCTGG 
GACTCGAGTC TGCACAACTC GGCACTGCTC AACCGCGTTT CTCAAGGCGG TGAGACCATC 
TACATCACCC TGAGCGCTTA CTTGGAGCTG GAGAATTGCG CCCGCCCGGC CATAATTACC 
AAGGACCTGA GCATGGTTAT CTATGGACGC GATGCCCGCA CCGGTCCGCG CTCCCTGAAG 
CACCTGTTTT CGGGACAGTA CCGCAATCCG GAGGCCAATC GCCTAACCGG GGTTTACGAG 
CTAGCACTGC GCAGAGCATC CGAAGCAGGT AGTCCAGGTG TACAAAGGCG TCAACGTCGA 
GTGCTGGACA CCAGCTCTAC TTATGTGCGC GGCGAGGAGA ATCTTCATGG CTGGAGGCCA 
AGGGGTGACT CCCTGATCTT CGACCACCAG TGGGAGCTGG AGAAACTCAC CAGACTTGAA 
GAGGTTGGAC GCATGCGGCA CTTGCTTCTG CTGCGCGAAC GTCTGGGCAT GGACACCAAC 
CCAAATCCGA CCACCAAGAC CGAGAAGGAT GTATGCAATC TAGCTGCTCG GGCAGCCACA 
TCACCCGTAC ATATGGTCAT TCCACAATCG CCGCAGACTC CGGTCAAGGA CCCACAGCAA 
ATCATTCCAG AACGCGAATA CAACCAACGG GAGCAGGATC TCATGCTTAA ATGCTTAAAG 
TTGGTGCAGG GACGCTATAC TAAGAGCGAG GCCAACGATA CGCAAACTCA GTCGGATGTT 
TCGCCTAGCG ATGAGGGATG CGCCGACATG ACCGTCAGCT GCATCTCCAG CAATTCCATG 
GAATTATGTT CACCGGATCG AGCTGATGCA CCCAATGGCT GGGAGGCACC TGCTCCGGCT 
ACTCAGCCGG CTCTTCCCCT CCGTCTCTAT GTGCCGGAAC TGGAGGAGAT TCGCGTGAGT 
CCTGTGGTGG CCCGCAAGGG TCTGTTGAAT GTCCTGGAGC ACGGCGGTTC CGGCTGGAAG 
AAGCGCTGGG TGATCGTCCG TCGCCCGTAC GTGTTTATCT ACCGCTCGGA GAAGGATCCC 
GTTGAACGGG CTGTCCTCAA TCTGGCGACT GCGCATGTGG AGTGCAGCGA GGACCAGGCG 
GCCATGGTCA AGATACCCAA CACATTCAGT GTGGTGACCA AGCATCGTGG CTATCTGCTG 
CAAACTCTCG GTGACAAGGA AGTACACGAC TGGCTGTATG CTATCAATCC ATTGCTTGCT 
GGGCAGATAA AATCCCGCCT GGCGCGACGG ACTTTGGAGC CTGCTAGCCA GACGGCTTCC 
CAGATCCAGG CCACCAATGC GGCGAATGCC AATAGTGCGA GCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246373.1
CDS	449..5476
Translation	MSSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGIEVQEGPDGKVVCEKRDANKDELTKSTVIKSPTKSRNRNGSTTEMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRQRLELMREMYHNEAEMSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEPGEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLVQGRYTKSEANDTQTQSDVSPSDEGCADMTVSCISSNSMELCSPDRADAPNGWEAPAPATQPALPLRLYVPELEEIRVSPVVARKGLLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARRTLEPASQTASQIQATNAANANSASK

Target ORF information:

RefSeq Version	NM_001259444.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated-104 (unc-104), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259444.2

ORF Insert Sequence:

ATGTCGTCGG TTAAGGTGGC GGTGCGAGTG CGCCCCTTCA ACTCGCGCGA AATAGCCAGG 
GAGTCGAAAT GCATCATCGA GATGGCAGGA GCCACAACGG CCATCACCAA TCCAAAGGTG 
CCGCCCAACA CAAGCGATTC AGTAAAGCGC TTCAACTTTG ATTACTCCTA CTGGTCACAT 
GATCACCACG ATGCCGATTT CTCCACACAA TCGATGGTGT ACAAGGACAT TGGCGAGGAG 
ATGTTACAGC ACTCCTTCGA TGGCTACAAT GTCTGTATCT TTGCCTATGG CCAGACTGGA 
GCTGGAAAGT CGTATACCAT GATGGGCAGG CAGGAGGAGC AGCAAGAGGG CATAATACCC 
ATGATATGCA AGGATCTGTT CACTCGCATA CAGGATACTG AGACCGATGA CCTCAAGTAC 
TCGGTTGAGG TTTCCTATAT GGAAATCTAT TGCGAGCGAG TCAGGGATTT GTTGAATCCG 
AAAAACAAGG GCAATCTGCG TGTGAGGGAG CATCCCCTTT TGGGTCCCTA TGTGGAGGAT 
TTGTCCAAAC TAGCCGTTAC CGATTACCAG GACATTCACG ATCTCATTGA TGAAGGCAAC 
AAAGCTCGAA CTGTGGCAGC TACTAACATG AACGAAACCA GTTCCCGCTC TCATGCCGTC 
TTTACAATCT TCTTCACCCA ACGTCGTCAT GATCTGATGA CCAATTTGAC CACAGAAAAG 
GTATCCAAGA TAAGCTTGGT AGACTTGGCC GGGTCGGAAC GAGCGGATTC CACTGGTGCC 
AAGGGCACTC GCTTGAAGGA GGGAGCCAAC ATTAACAAGT CCTTGACCAC ATTGGGCAAA 
GTTATCTCAG CTTTGGCGGA AGTGGCTTCC AAGAAAAAGA ACACCAAGAA GGCAGATTTT 
ATTCCGTACC GTGATTCGGC CTTGACCTGG CTGTTACGTG AAAACTTGGG AGGAAATTCG 
AAGACAGCTA TGATTGCGGC TATCTCACCA GCAGATATTA ACTACGATGA AACCCTCAGC 
ACACTGCGCT ATGCGGATCG TGCCAAGCAA ATTGTTTGCA AGGCTGTGGT CAATGAAGAT 
GCCAATGCCA AGCTGATTCG CGAACTCAAG GAGGAGATCC AGAAGCTGCG GGACTTACTC 
AAAGCCGAGG GCATTGAAGT GCAGGAAGGA CCCGATGGCA AAGTGGTGTG TGAGAAGCGC 
GATGCGAATA AGGATGAGCT CACCAAGTCA ACGGTGATCA AGTCGCCCAC TAAGTCACGT 
AATCGTAATG GATCCACCAC GGAGATGGCT GTGGATCAGC TGCAGGCCAG CGAGAAACTT 
ATTGCAGAGC TCAACGAGAC CTGGGAGGAG AAACTTAAGC GCACCGAGGA GATTCGCGTG 
CAGCGAGAGG CGGTCTTCGC CGAAATGGGT GTGGCTGTCA AGGAAGATGG AATAACAGTT 
GGCGTATTCT CACCGAAGAA GACTCCGCAT TTGGTCAACC TAAACGAGGA TCCCAATCTG 
TCTGAGTGTC TGCTTTACTA CATCAAGGAG GGTCTAACTC GGTTGGGTAC CCATGAAGCA 
AATGTGCCCC AGGACATTCA GCTCTCCGGA TCGCACATCC TCAAAGAGCA CTGCACCTTT 
GAGAACAAGA ACAGCACGGT TACCCTGCTG CCACACAAGG ATGCTATCAT CTATGTAAAT 
GGACGCAAGT TGGTTGAACC GGAGGTTCTT AAGACCGGTT CTCGCGTGAT CCTCGGAAAG 
AACCACGTGT TCCGCTTTAC CAATCCAGAA CAGGCACGCG AATTACGGGA TAAGATCGAG 
ACCGAAAATG AGGCTGAGAA CGAAGTGGAG AAGACAGACA CCCAGCAGGT GGACTGGAAT 
TTTGCCCAGT GCGAATTGCT CGAGAAGCAA GGCATTGATC TTAAAGCTGA AATGAAGAAG 
CGTTTAGACA ACTTGGAGGA ACAATACAAG CGGGAGAAAC TTCAGGCCGA TCAGCAATTC 
GAGGAGCAGC GCAAAACGTA CGAGGCTCGC ATCGATGCTT TGCAAAAACA GGTTGAGGAG 
CAATCCATGA CCATGTCCAT GTACAGCAGC TACTCGCCGG AGGATTTCCA CCAGGAGGAG 
GACGTCTACA CCAACCCCAT GTACGAATCC TGCTGGACAG CAAGAGAGGC TGGCTTGGCT 
GCCTGGGCTT TCCGCAAGTG GCGTTACCAC CAATTCACCT CTTTGCGAGA TGATCTCTGG 
GGCAATGCTA TATTCCTTAA GGAAGCGAAT GCCATTTCCG TTGAGTTAAA GAAGAAGGTA 
CAATTCCAAT TTACTCTCTT GACCGACACC TTGTACTCTC CTTTGCCGCC TGAGCTGGCA 
TCCACTGTGG CTCCTGTGCA TCAGGAGGAT GAGTTCGGAG CTCCACCTGT CTCTAAGACC 
TTGGTGGCCG TCGAAGTAAC CGATACTAAG AACGGAGCCA CTCACCACTG GTCTCTGGAG 
AAGTTACGGC AACGTTTGGA GCTGATGCGC GAGATGTACC ACAACGAAGC CGAAATGAGT 
CCCACTTCAC CGGACTATAA TGTGGAGAGC CTCACTGGTG GAGATCCATT CTACGATCGC 
TTCCCCTGGT TCCGCATGGT GGGTCGCTCC TTCATCTATC TGAGCAACCT GCTCTACCCA 
GTGCCATTGG TCCACAAGGT GGCCATTGTC AACGAACGCG GAGATGTGCG TGGCTACCTA 
AGGATTGCTG TGCAGCCCGT TCTGGATGAG GAGTCCATTG ATTTCAATAA TGGTGTCAAG 
CAGTCAGCTC GCTTGGTTTT CAATGAGGAT GATGCCAAAC CGAAATACCG AGCTCTCAAT 
GAAAAGGATG ATGTGCAGCG CTATATTGAC AATGGTGGTT TGGACAGCAA GCTGGAGGAA 
CTTGAGGATG TGGACTCTGG TCGTGGCATT GACTCTAACT CCGCATCCGA GTGCCACGAA 
AATTCGGAGG AGCCTGGCGA GCATCTGCAG GTGGGCAAGG AGTTCACTTT CCGGGTCACT 
GTGCTACAGG CCACTGGTAT TGGGGCTGAA TATGCCGATA TCTTCTGCCA GTTCAACTTT 
TTGCATCGTC ATGAGGAGGC TTTCTCCACC GAACCCGTCA AGAATTCGGC ATCGGGTGCT 
CCTCTAGGCT TCTACCATGT GCAGAACATA ACTGTACCCG TAACCAAATC CTTCATCGAG 
TATTTGAAGA CCCAACCCAT AATGTTCAAG ATATTTGGGC ACTACCAGAC GCACCCATTG 
CATAAGGATG CCAAACAGGA TTTTGTTTCT CGGCCACCAC CACGTCGCAT GTTGCCACCG 
AGCATACCGA TCAGTCAGCC AGTGCGTAGT CCCAAGTTTG GACCACTACC GTGTGCACCC 
ACGTCTACGG TTTTGGCTAA GCACGATGTT TTGGTTTGGT TTGAAATCTG TGAATTGGCT 
CCCAATGGAG AATATGTGCC ATCGGTGGTG GAGCACAGCG ATGATCTTCC CTGCCGCGGA 
CTGTTCCTCT TGCATCAGGG CATCCAGCGG CGCATTCGTA TTACTATCGT ACATGAGCCC 
ACAACCGAGG TGAAGTGGAA GGACATCAAT GAGTTGGTGG TTGGACGTAT CCGCAATACA 
CCGGAGTCAT CCGACGAACA GGACGAAGAC GCCTGTGTCT TATCTTTGGG CTTGTTCCCA 
GGCGAGGCTT TGGAGGTGCC CGGAGATGAT AGATCTTTCT ACCGTTTTGA GGCTGCCTGG 
GACTCGAGTC TGCACAACTC GGCACTGCTC AACCGCGTTT CTCAAGGCGG TGAGACCATC 
TACATCACCC TGAGCGCTTA CTTGGAGCTG GAGAATTGCG CCCGCCCGGC CATAATTACC 
AAGGACCTGA GCATGGTTAT CTATGGACGC GATGCCCGCA CCGGTCCGCG CTCCCTGAAG 
CACCTGTTTT CGGGACAGTA CCGCAATCCG GAGGCCAATC GCCTAACCGG GGTTTACGAG 
CTAGCACTGC GCAGAGCATC CGAAGCAGGT AGTCCAGGTG TACAAAGGCG TCAACGTCGA 
GTGCTGGACA CCAGCTCTAC TTATGTGCGC GGCGAGGAGA ATCTTCATGG CTGGAGGCCA 
AGGGGTGACT CCCTGATCTT CGACCACCAG TGGGAGCTGG AGAAACTCAC CAGACTTGAA 
GAGGTTGGAC GCATGCGGCA CTTGCTTCTG CTGCGCGAAC GTCTGGGCAT GGACACCAAC 
CCAAATCCGA CCACCAAGAC CGAGAAGGAT GTATGCAATC TAGCTGCTCG GGCAGCCACA 
TCACCCGTAC ATATGGTCAT TCCACAATCG CCGCAGACTC CGGTCAAGGA CCCACAGCAA 
ATCATTCCAG AACGCGAATA CAACCAACGG GAGCAGGATC TCATGCTTAA ATGCTTAAAG 
TTGGTGCAGG GACGCTATAC TAAGAGCGAG GCCAACGATA CGCAAACTCA GTCGGATGTT 
TCGCCTAGCG ATGAGGGATG CGCCGACATG ACCGTCAGCT GCATCTCCAG CAATTCCATG 
GAATTATGTT CACCGGATCG AGCTGATGCA CCCAATGGCT GGGAGGCACC TGCTCCGGCT 
ACTCAGCCGG CTCTTCCCCT CCGTCTCTAT GTGCCGGAAC TGGAGGAGAT TCGCGTGAGT 
CCTGTGGTGG CCCGCAAGGG TCTGTTGAAT GTCCTGGAGC ACGGCGGTTC CGGCTGGAAG 
AAGCGCTGGG TGATCGTCCG TCGCCCGTAC GTGTTTATCT ACCGCTCGGA GAAGGATCCC 
GTTGAACGGG CTGTCCTCAA TCTGGCGACT GCGCATGTGG AGTGCAGCGA GGACCAGGCG 
GCCATGGTCA AGATACCCAA CACATTCAGT GTGGTGACCA AGCATCGTGG CTATCTGCTG 
CAAACTCTCG GTGACAAGGA AGTACACGAC TGGCTGTATG CTATCAATCC ATTGCTTGCT 
GGGCAGATAA AATCCCGCCT GGCGCGACGG ACTTTGGAGC CTGCTAGCCA GACGGCTTCC 
CAGATCCAGG CCACCAATGC GGCGAATGCC AATAGTGCGA GCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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unc-104 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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