Cdk4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdk4
Entrez Gene ID	36854
Full Name	Cyclin-dependent kinase 4
Synonyms	8-6,CDK4,CDK4/6,CG5072,Cdk4/6,DmCdk4,Dmel\CG5072,Pk53C,Pk?7,cdk,cdk4,cdk4/6,dCdk4,l(2)05428,l(2)0671,l(2)k06503,l(2)s4639,l(2)sh0671
General protein information	Preferred Names Cyclin-dependent kinase 4 Names CG5072 gene product from transcript CG5072-RC CG5072-PA CG5072-PB CG5072-PC CG5072-PD CG5072-PE Cdk4-PA Cdk4-PB Cdk4-PC Cdk4-PD Cdk4-PE Cyclin-dependent kinase 4/6 cyclin-dependent kinase 4 protein kinase-like 53C
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53C9-53C9

mRNA and Protein(s)

mRNA	Protein	Name
NM_057848.3	NP_477196.1	Cyclin-dependent kinase 4, isoform B
NM_001299565.1	NP_001286494.1	Cyclin-dependent kinase 4, isoform E
NM_166185.2	NP_725595.1	Cyclin-dependent kinase 4, isoform C
NM_166184.2	NP_725594.1	Cyclin-dependent kinase 4, isoform A
NM_001299564.1	NP_001286493.1	Cyclin-dependent kinase 4, isoform D

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cdk6	NP_001137525.1
Drosophila melanogaster (fruit fly)	Cdk4	NP_725595.1
Xenopus tropicalis (tropical clawed frog)	cdk6	XP_002934637.2
	CDK6	XP_001100307.1
Canis lupus familiaris (dog)	CDK6	XP_852360.1
Bos taurus (cattle)	CDK6	NP_001179230.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005817	XP_315841.3
Homo sapiens (human)	CDK6	NP_001138778.1
Gallus gallus (chicken)	CDK6	NP_001007893.1
Pan troglodytes (chimpanzee)	CDK6	XP_003318627.1
Mus musculus (house mouse)	Cdk6	NP_034003.1
Rattus norvegicus (Norway rat)	Cdk6	NP_001178790.1
Caenorhabditis elegans (roundworm)	cdk-4	NP_510256.1
Caenorhabditis elegans (roundworm)	cdk-4	NP_001024591.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results indicate that the precise regulation of neuronal Cdk4 activity is important to limit mitochondrial reactive oxygen species production and prevent neurodegeneration.
Title: Changes in neuronal CycD/Cdk4 activity affect aging, neurodegeneration, and oxidative stress.

CDK4 activity regulates mitobiogenesis by the activation of NRF-1 and consequent induction of Tfam and mitochondrial ribossomal transcription.
Title: Drosophila cyclin D/Cdk4 regulates mitochondrial biogenesis and aging and sensitizes animals to hypoxic stress.

Delg and cyclin D/Cdk4 have roles in nutritional control of mitochondrial biogenesis in the Drosophila adipose tissue
Title: Nutrition controls mitochondrial biogenesis in the Drosophila adipose tissue through Delg and cyclin D/Cdk4.

Our data suggest that the growth-specific function of CycD/Cdk4 is conserved from arthropods to mammals.
Title: Mammalian cyclin D1/Cdk4 complexes induce cell growth in Drosophila.

mRpL12 is required for CycD/Cdk4-induced cell growth
Title: The Drosophila mitochondrial ribosomal protein mRpL12 is required for Cyclin D/Cdk4-driven growth.

The following Cdk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10244	NM_001299564.1 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10244	NM_166185.2 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10244	NM_057848.3 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10244	NM_001299565.1 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10244	NM_166184.2 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdk4 ( NM_057848.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477196.1 Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10244
Clone ID Related Accession (Same CDS sequence)	NM_057848.3 , NM_001299565.1 , NM_166185.2 , NM_166184.2 , NM_001299564.1
Accession Version	NM_057848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057848.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477196.1
CDS	660..1613
Translation	MSYVRQLKRQKMSQAKKFGDGDPFNYQELNIIGEGAYGTVYRARDVITGNIVALKKVRISLNENGVPMSTLREISLLKQLNASNHANIVKLYEVCQFLERDGQLLILLVFEHVEQDLSDLIDRLPKSGMSPPTIQRLSRELLTGVDFLHSHRIIHRDLKPQNLLVSSQGHLKIADFGLAKTYGSEMKLTSVVVTLWYRAPEVLLAQPYNSTVDIWSAACIIFEMFNRRALFPGTSEKNQLDRIFELTGRPTEQQWPQTISVALEHFPQRHPKRPKDFCPHLCKYADDLLNKMLSYDLHLRPSALACLEHDYFQQEPL

Target ORF information:

RefSeq Version	NM_057848.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057848.3

ORF Insert Sequence:

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk4 ( NM_001299565.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286494.1 Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa10244
Clone ID Related Accession (Same CDS sequence)	NM_057848.3 , NM_001299565.1 , NM_166185.2 , NM_166184.2 , NM_001299564.1
Accession Version	NM_001299565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 4, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286494.1
CDS	234..1187
Translation	MSYVRQLKRQKMSQAKKFGDGDPFNYQELNIIGEGAYGTVYRARDVITGNIVALKKVRISLNENGVPMSTLREISLLKQLNASNHANIVKLYEVCQFLERDGQLLILLVFEHVEQDLSDLIDRLPKSGMSPPTIQRLSRELLTGVDFLHSHRIIHRDLKPQNLLVSSQGHLKIADFGLAKTYGSEMKLTSVVVTLWYRAPEVLLAQPYNSTVDIWSAACIIFEMFNRRALFPGTSEKNQLDRIFELTGRPTEQQWPQTISVALEHFPQRHPKRPKDFCPHLCKYADDLLNKMLSYDLHLRPSALACLEHDYFQQEPL

Target ORF information:

RefSeq Version	NM_001299565.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299565.1

ORF Insert Sequence:

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk4 ( NM_166185.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725595.1 Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10244
Clone ID Related Accession (Same CDS sequence)	NM_057848.3 , NM_001299565.1 , NM_166185.2 , NM_166184.2 , NM_001299564.1
Accession Version	NM_166185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166185.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725595.1
CDS	136..1089
Translation	MSYVRQLKRQKMSQAKKFGDGDPFNYQELNIIGEGAYGTVYRARDVITGNIVALKKVRISLNENGVPMSTLREISLLKQLNASNHANIVKLYEVCQFLERDGQLLILLVFEHVEQDLSDLIDRLPKSGMSPPTIQRLSRELLTGVDFLHSHRIIHRDLKPQNLLVSSQGHLKIADFGLAKTYGSEMKLTSVVVTLWYRAPEVLLAQPYNSTVDIWSAACIIFEMFNRRALFPGTSEKNQLDRIFELTGRPTEQQWPQTISVALEHFPQRHPKRPKDFCPHLCKYADDLLNKMLSYDLHLRPSALACLEHDYFQQEPL

Target ORF information:

RefSeq Version	NM_166185.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166185.2

ORF Insert Sequence:

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk4 ( NM_166184.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725594.1 Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10244
Clone ID Related Accession (Same CDS sequence)	NM_057848.3 , NM_001299565.1 , NM_166185.2 , NM_166184.2 , NM_001299564.1
Accession Version	NM_166184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166184.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725594.1
CDS	435..1388
Translation	MSYVRQLKRQKMSQAKKFGDGDPFNYQELNIIGEGAYGTVYRARDVITGNIVALKKVRISLNENGVPMSTLREISLLKQLNASNHANIVKLYEVCQFLERDGQLLILLVFEHVEQDLSDLIDRLPKSGMSPPTIQRLSRELLTGVDFLHSHRIIHRDLKPQNLLVSSQGHLKIADFGLAKTYGSEMKLTSVVVTLWYRAPEVLLAQPYNSTVDIWSAACIIFEMFNRRALFPGTSEKNQLDRIFELTGRPTEQQWPQTISVALEHFPQRHPKRPKDFCPHLCKYADDLLNKMLSYDLHLRPSALACLEHDYFQQEPL

Target ORF information:

RefSeq Version	NM_166184.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166184.2

ORF Insert Sequence:

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk4 ( NM_001299564.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286493.1 Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant D, mRNA.

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CloneID	OFa10244
Clone ID Related Accession (Same CDS sequence)	NM_057848.3 , NM_001299565.1 , NM_166185.2 , NM_166184.2 , NM_001299564.1
Accession Version	NM_001299564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 4, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286493.1
CDS	81..1034
Translation	MSYVRQLKRQKMSQAKKFGDGDPFNYQELNIIGEGAYGTVYRARDVITGNIVALKKVRISLNENGVPMSTLREISLLKQLNASNHANIVKLYEVCQFLERDGQLLILLVFEHVEQDLSDLIDRLPKSGMSPPTIQRLSRELLTGVDFLHSHRIIHRDLKPQNLLVSSQGHLKIADFGLAKTYGSEMKLTSVVVTLWYRAPEVLLAQPYNSTVDIWSAACIIFEMFNRRALFPGTSEKNQLDRIFELTGRPTEQQWPQTISVALEHFPQRHPKRPKDFCPHLCKYADDLLNKMLSYDLHLRPSALACLEHDYFQQEPL

Target ORF information:

RefSeq Version	NM_001299564.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 4 (Cdk4), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299564.1

ORF Insert Sequence:

ATGTCATATG TACGCCAGCT GAAGCGGCAG AAAATGTCCC AGGCGAAGAA GTTCGGCGAT 
GGCGATCCGT TCAACTACCA GGAGCTCAAC ATCATCGGCG AAGGTGCCTA TGGAACGGTT 
TACAGAGCCC GCGATGTGAT CACCGGCAAC ATAGTGGCGC TGAAGAAGGT TAGGATATCG 
CTGAACGAGA ACGGTGTGCC AATGTCCACG CTGCGGGAGA TTTCGCTGCT GAAGCAGCTG 
AATGCCAGCA ATCATGCGAA TATTGTCAAA CTGTACGAAG TATGCCAGTT TCTGGAGCGC 
GACGGCCAAT TGCTGATTCT GTTGGTTTTC GAACATGTGG AGCAGGATCT TTCGGATCTT 
ATAGACAGAC TTCCCAAATC GGGCATGTCG CCGCCCACAA TTCAGCGCTT GTCAAGGGAA 
CTACTGACCG GTGTGGACTT CTTACACTCA CACCGTATCA TCCATCGGGA TCTTAAGCCG 
CAAAACCTGC TGGTCTCCTC GCAGGGCCAC CTCAAGATCG CCGACTTTGG CTTGGCCAAG 
ACCTACGGTT CGGAGATGAA GCTCACCTCC GTGGTGGTCA CCTTGTGGTA CCGCGCACCC 
GAGGTTCTGC TCGCCCAGCC CTACAACAGC ACCGTGGACA TCTGGAGTGC CGCCTGCATC 
ATCTTCGAGA TGTTCAATCG GCGCGCCCTA TTCCCGGGCA CCTCCGAAAA GAATCAGCTG 
GACCGCATCT TCGAACTGAC CGGTAGACCC ACCGAGCAAC AGTGGCCACA GACCATTTCC 
GTGGCCCTGG AACACTTTCC CCAGCGGCAT CCAAAGAGAC CAAAAGACTT TTGCCCACAC 
CTGTGCAAGT ACGCCGATGA TCTGTTGAAT AAAATGCTTT CCTACGACCT CCACCTGCGA 
CCATCCGCCT TGGCCTGTTT GGAGCACGAT TACTTTCAGC AGGAACCCCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cdk4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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