krimp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	krimp
Entrez Gene ID	36804
Full Name	krimper
Synonyms	CG15707,Dmel\CG15707,KRIMP,Krimp,mtc
General protein information	Preferred Names krimper Names CG15707 gene product from transcript CG15707-RA CG15707-PA krimp-PA montecristo
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53A1-53A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_137259.4	NP_611103.2	krimper

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Krimp enforces an antisense bias on piRNA pools by binding AGO3 and blocking its access to primary piRNAs.
Title: Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline.

Krimper interacts directly with Aub and Ago3 to coordinate the assembly of the ping-pong piRNA processing complex.
Title: Aub and Ago3 Are Recruited to Nuage through Two Mechanisms to Form a Ping-Pong Complex Assembled by Krimper.

Krimper and Qin proteins play crucial roles in maintaining the levels of piRNAs loaded onto Piwi and Aub to efficiently repress transposons at transcriptional and post-transcriptional levels, respectively.
Title: Tudor-domain containing proteins act to make the piRNA pathways more robust in Drosophila.

Krimper, a component of the nuage, ensures the repression of selfish genetic elements in the female germ line.
Title: Unique germ-line organelle, nuage, functions to repress selfish genetic elements in Drosophila melanogaster.

The following krimp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the krimp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10193	NM_137259.4 Latest version!	Drosophila melanogaster krimper (krimp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

krimp ( NM_137259.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611103.2 Drosophila melanogaster krimper (krimp), mRNA.

Price & Availability↑

CloneID	OFa10193
Clone ID Related Accession (Same CDS sequence)	NM_137259.4
Accession Version	NM_137259.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	krimper
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137259.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGG AGGACATTTC GATGATTATG AAGCTGTTCG ATAGCAACAT GCACAAGCTA 
CAGGGGAATC TACGTTCCTA TCAAACCGAA ATGCATCAAA TTCACAAGGA GCTCACAGAA 
AAATTGTCGC ATGCAGATCT GCTGTACAGG AGTCTCATTC CGCTGCACGA TCACTTGGTG 
GCCAGTCTGA GCGAAGTTAA TGCACACGTA ATGAAACTGA ATGTGCAACT ACACATCAAC 
CGCCAGTCTG TCAGATTGGG AGACTACGAA TATTACGAGA AATCCATCGA CAATCCATAT 
TCGTCGATTC GCTCAGGTTT ACAAGCGATC GAAAAACCGG GTTGTGCTGA AGCTATATGT 
CAGTCCTCGA AGCCGGCCTT CGTGGAATGT CTGCCCTCCT CCACTTCGGA GGAAGTGCCC 
GTCGTGGCTG TGCAGGAAGC CTCTTCGACA AACCAGCTCG ACGCAATATC AGTGGTCAAT 
GAAAACCTCT CAGAAGAGAG AGACGCTACT CCGCAGCCTT TAGCAGTTTC CAAGAACATG 
GAGGAGACTA TGCCCTCGAA TCCTTTCCAC GAACAGCTTG AGGGATCTCT AGAAGAGATC 
CCAGTGGGCT CCAAGATTGT CGTGGAAACT GAAAAGGCCA ACAATCCAGT ACGTTCCGAA 
GCTTCCGCTC CAGCAACTTC TGACAATCAG TCCCTTCTGG CTGCAAAACA GGGAACTCAA 
ACAATTGGCA CAGGTATCTG CAACAAGATC TCCAAGAGCA CCATCAACAT GCCCAACAAC 
TGGCAGCTTG AGAATCCTAC TGAGGTAACT GCTGCATCGA TCGAAAAGGT TAACAAGCTC 
CCAAAAAGTC CCAGAAACCG ATTCCTATTG CCTCCCAAGG GAGGCACTGA GACGACCAGA 
CGTGATATCT ACAATCAAAT CTTAAAGGAC ATGGCTGCAT TTCCAGAGAA TACTATAGTT 
ACTGCCGTTT TAGCGAGCGT GGACGTAACG GATAATTGCG CCTATGTGGC TAAATGGGAT 
GAAAGTTCTG ATAGGATTAA GAAGGTGCTC CAGCGACAAC TGCCATTGCA GGAGTTGGAC 
CAGTTGCCCG ACTACGGCGA CATTTTTGCA GTTCTCGACA GTATTAACAA CATCATTACT 
CGGATCACCA TCAACTCAAG TTCTGCGGGC GGAGGATATG ATGCGTATCT TATCGACTTT 
GGCGAGCATA TTCATTTTGA CGGCAACGAA ACAATATTTA AACTACCGGA CGACATCAAA 
CGGTTGCCGG CACAGGCCAT TAGATGCGAT TTAATCAACT GTGATATCGC CAATATGCAT 
TGCTTTGTTA ATACGTATAT TAAGATTCGA GTGCACGAAA ACAATAATTC TACACTAGTG 
GCCGAACCGG TCATTGATAG GTTAAGCAGA CCTACAAAAA CGAACACCAC TAAATATCCA 
GCTGGAATAA CCGAGGATGA CATGGCCATG CTGAATGAAA TCGATGAGAG CACCAGTGAT 
CCTCTGAAAG CAGTTCTTGG CTTTCGTCCC AAGGACGAGC AACGCATTTG CCGGCACTAC 
GATCCCAAGC TGAATGGCTG TTTTAAGGGT AACAATTGTC GTTTTGCTCA TGAGCCCTTT 
GCTCCGAATG GCGCCACCAA AGATGTGGAG CTAGCTAGAG CTCTTCCTGA GACAATCTTT 
GATACAACAG TGCATTTTGA AATCGGCAGC ATTGTCGGCA TCCTGATCAC CTTTATTAAT 
GGCCCCACCG AGGTTTATGG CCAGTTTTTG GACGGATCTC CGCCGTTGGT GTGGGACAAA 
AAGGATGTGC CAGAGAACAA ACGGACCTTC AAGTCGAAAC CGCGTCTTCT TGACATTGTA 
CTTGCTCTCT ACAGTGATGG TTGCTTCTAT CGCGCCCAGA TAATCGATGA ATTCCCCAGT 
GAGTACATGA TCTTCTATGT GGATTACGGC AACACGGAGT TCGTGCCCCT AAGCTGCTTG 
GCTCCATGTG AAAACGTGGA CAGCTTTAAG CCCCACCGCG TCTTTAGTTT TCACATTGAG 
GGAATTGTAA GGTCAAAAAA TCTGACACAC CAAAAGACAA TCGAATGCAT CGAGTATCTC 
AAGAGTAAGC TGCTAAACAC GGAGATGAAT GTACATCTGG TACAGCGCCT ACCGGATGGC 
TTCCTAATTC GCTTCCTGGA CGATTGGAAA TATATACCTG AACAGCTATT GCAACGCAAC 
TACGCTCAGG TTAGCCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611103.2
CDS	78..2318
Translation	MNLEDISMIMKLFDSNMHKLQGNLRSYQTEMHQIHKELTEKLSHADLLYRSLIPLHDHLVASLSEVNAHVMKLNVQLHINRQSVRLGDYEYYEKSIDNPYSSIRSGLQAIEKPGCAEAICQSSKPAFVECLPSSTSEEVPVVAVQEASSTNQLDAISVVNENLSEERDATPQPLAVSKNMEETMPSNPFHEQLEGSLEEIPVGSKIVVETEKANNPVRSEASAPATSDNQSLLAAKQGTQTIGTGICNKISKSTINMPNNWQLENPTEVTAASIEKVNKLPKSPRNRFLLPPKGGTETTRRDIYNQILKDMAAFPENTIVTAVLASVDVTDNCAYVAKWDESSDRIKKVLQRQLPLQELDQLPDYGDIFAVLDSINNIITRITINSSSAGGGYDAYLIDFGEHIHFDGNETIFKLPDDIKRLPAQAIRCDLINCDIANMHCFVNTYIKIRVHENNNSTLVAEPVIDRLSRPTKTNTTKYPAGITEDDMAMLNEIDESTSDPLKAVLGFRPKDEQRICRHYDPKLNGCFKGNNCRFAHEPFAPNGATKDVELARALPETIFDTTVHFEIGSIVGILITFINGPTEVYGQFLDGSPPLVWDKKDVPENKRTFKSKPRLLDIVLALYSDGCFYRAQIIDEFPSEYMIFYVDYGNTEFVPLSCLAPCENVDSFKPHRVFSFHIEGIVRSKNLTHQKTIECIEYLKSKLLNTEMNVHLVQRLPDGFLIRFLDDWKYIPEQLLQRNYAQVSQ

Target ORF information:

RefSeq Version	NM_137259.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster krimper (krimp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137259.4

ORF Insert Sequence:

ATGAATCTGG AGGACATTTC GATGATTATG AAGCTGTTCG ATAGCAACAT GCACAAGCTA 
CAGGGGAATC TACGTTCCTA TCAAACCGAA ATGCATCAAA TTCACAAGGA GCTCACAGAA 
AAATTGTCGC ATGCAGATCT GCTGTACAGG AGTCTCATTC CGCTGCACGA TCACTTGGTG 
GCCAGTCTGA GCGAAGTTAA TGCACACGTA ATGAAACTGA ATGTGCAACT ACACATCAAC 
CGCCAGTCTG TCAGATTGGG AGACTACGAA TATTACGAGA AATCCATCGA CAATCCATAT 
TCGTCGATTC GCTCAGGTTT ACAAGCGATC GAAAAACCGG GTTGTGCTGA AGCTATATGT 
CAGTCCTCGA AGCCGGCCTT CGTGGAATGT CTGCCCTCCT CCACTTCGGA GGAAGTGCCC 
GTCGTGGCTG TGCAGGAAGC CTCTTCGACA AACCAGCTCG ACGCAATATC AGTGGTCAAT 
GAAAACCTCT CAGAAGAGAG AGACGCTACT CCGCAGCCTT TAGCAGTTTC CAAGAACATG 
GAGGAGACTA TGCCCTCGAA TCCTTTCCAC GAACAGCTTG AGGGATCTCT AGAAGAGATC 
CCAGTGGGCT CCAAGATTGT CGTGGAAACT GAAAAGGCCA ACAATCCAGT ACGTTCCGAA 
GCTTCCGCTC CAGCAACTTC TGACAATCAG TCCCTTCTGG CTGCAAAACA GGGAACTCAA 
ACAATTGGCA CAGGTATCTG CAACAAGATC TCCAAGAGCA CCATCAACAT GCCCAACAAC 
TGGCAGCTTG AGAATCCTAC TGAGGTAACT GCTGCATCGA TCGAAAAGGT TAACAAGCTC 
CCAAAAAGTC CCAGAAACCG ATTCCTATTG CCTCCCAAGG GAGGCACTGA GACGACCAGA 
CGTGATATCT ACAATCAAAT CTTAAAGGAC ATGGCTGCAT TTCCAGAGAA TACTATAGTT 
ACTGCCGTTT TAGCGAGCGT GGACGTAACG GATAATTGCG CCTATGTGGC TAAATGGGAT 
GAAAGTTCTG ATAGGATTAA GAAGGTGCTC CAGCGACAAC TGCCATTGCA GGAGTTGGAC 
CAGTTGCCCG ACTACGGCGA CATTTTTGCA GTTCTCGACA GTATTAACAA CATCATTACT 
CGGATCACCA TCAACTCAAG TTCTGCGGGC GGAGGATATG ATGCGTATCT TATCGACTTT 
GGCGAGCATA TTCATTTTGA CGGCAACGAA ACAATATTTA AACTACCGGA CGACATCAAA 
CGGTTGCCGG CACAGGCCAT TAGATGCGAT TTAATCAACT GTGATATCGC CAATATGCAT 
TGCTTTGTTA ATACGTATAT TAAGATTCGA GTGCACGAAA ACAATAATTC TACACTAGTG 
GCCGAACCGG TCATTGATAG GTTAAGCAGA CCTACAAAAA CGAACACCAC TAAATATCCA 
GCTGGAATAA CCGAGGATGA CATGGCCATG CTGAATGAAA TCGATGAGAG CACCAGTGAT 
CCTCTGAAAG CAGTTCTTGG CTTTCGTCCC AAGGACGAGC AACGCATTTG CCGGCACTAC 
GATCCCAAGC TGAATGGCTG TTTTAAGGGT AACAATTGTC GTTTTGCTCA TGAGCCCTTT 
GCTCCGAATG GCGCCACCAA AGATGTGGAG CTAGCTAGAG CTCTTCCTGA GACAATCTTT 
GATACAACAG TGCATTTTGA AATCGGCAGC ATTGTCGGCA TCCTGATCAC CTTTATTAAT 
GGCCCCACCG AGGTTTATGG CCAGTTTTTG GACGGATCTC CGCCGTTGGT GTGGGACAAA 
AAGGATGTGC CAGAGAACAA ACGGACCTTC AAGTCGAAAC CGCGTCTTCT TGACATTGTA 
CTTGCTCTCT ACAGTGATGG TTGCTTCTAT CGCGCCCAGA TAATCGATGA ATTCCCCAGT 
GAGTACATGA TCTTCTATGT GGATTACGGC AACACGGAGT TCGTGCCCCT AAGCTGCTTG 
GCTCCATGTG AAAACGTGGA CAGCTTTAAG CCCCACCGCG TCTTTAGTTT TCACATTGAG 
GGAATTGTAA GGTCAAAAAA TCTGACACAC CAAAAGACAA TCGAATGCAT CGAGTATCTC 
AAGAGTAAGC TGCTAAACAC GGAGATGAAT GTACATCTGG TACAGCGCCT ACCGGATGGC 
TTCCTAATTC GCTTCCTGGA CGATTGGAAA TATATACCTG AACAGCTATT GCAACGCAAC 
TACGCTCAGG TTAGCCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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krimp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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