CysRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CysRS
Entrez Gene ID	36784
Full Name	Cysteinyl-tRNA synthetase
Synonyms	Aats-cys,BcDNA:LD21177,CG8431,CRS,Dmel\CG8431
General protein information	Preferred Names Cysteinyl-tRNA synthetase Names CG8431 gene product from transcript CG8431-RA CG8431-PA CysRS-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52E11-52E11

mRNA and Protein(s)

mRNA	Protein	Name
NM_137243.3	NP_611087.1	Cysteinyl-tRNA synthetase

Pathways from BioSystems

Homologies

	CARS	XP_001096302.1
Oryza sativa (rice)	Os09g0556500	NP_001063899.1
Homo sapiens (human)	CARS	NP_001014437.1
Rattus norvegicus (Norway rat)	Cars	NP_001099789.1
Drosophila melanogaster (fruit fly)	Aats-cys	NP_611087.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012283	XP_320253.2
Arabidopsis thaliana (thale cress)	SYCO ARATH	NP_565717.2
Mus musculus (house mouse)	Cars	NP_038770.3
Gallus gallus (chicken)	CARS	NP_001012601.1
Caenorhabditis elegans (roundworm)	cars-1	NP_491014.1
Saccharomyces cerevisiae (baker's yeast)	YNL247W	NP_014152.1
Kluyveromyces lactis	KLLA0C03025g	XP_452332.1
Pan troglodytes (chimpanzee)	CARS	XP_003312912.1
Canis lupus familiaris (dog)	CARS	XP_851224.1
Bos taurus (cattle)	CARS	NP_001096719.1
Xenopus tropicalis (tropical clawed frog)	cars	NP_001011365.1
Danio rerio (zebrafish)	cars	NP_001112372.1
Schizosaccharomyces pombe (fission yeast)	SPAC29E6.06c	NP_594564.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CysRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CysRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10173	NM_137243.3 Latest version!	Drosophila melanogaster Cysteinyl-tRNA synthetase (CysRS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CysRS ( NM_137243.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611087.1 Drosophila melanogaster Cysteinyl-tRNA synthetase (CysRS), mRNA.

Price & Availability↑

CloneID	OFa10173
Clone ID Related Accession (Same CDS sequence)	NM_137243.3
Accession Version	NM_137243.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cysteinyl-tRNA synthetase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137243.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAAC GTGGGCAACC GGCGTGGCAA GCTCCCGAGG CGGTGGATCG GCCGAAACTG 
AAGCTGTTCA ACAGCCTGAC GCGACAGAAG GAGGACTTCG TGCCGCTGGA CGGCAATAAT 
GTAACGTGGT ATAGCTGCGG ACCCACCGTC TACGATGCCT CACACATGGG GCATGCTAGA 
TCTTACATTT CCTTCGACAT TCTGCGGCGC ATTCTGTCCG ACTACTTTGG CTACAACATT 
CACTATGTGA TGAACATTAC GGACATCGAC GACAAGATCA TAAGGCGTGC GCGGCAGAAT 
CATCTCTTCG ACGAGTACGC TGCTGAGGCG CAAAAGCTGC CACTGGATGA GCTGCTGGGT 
CAGCAAAAGG AGGTACTGCA GCGGTTTCAG GATACATGCG CCAAGAATAC AGATCCCGAC 
AAGAAGATTA TGCTCGACAA GACACTGCAG CGGATGAACG ATGCTGTGGA GGCGCTGACC 
AAGGCCGTTG GCAAGGGTGA TGAGCGGGAG ATATCCGAGA AGCGGCTGCT TTACCTAAAC 
GAAGCCAAGG ATCCAATTTC CGACTGGTTG GACTCGCTAA AGGGTGCTCA GATCAATGAT 
AATGCAGTAT TTGAGGCACT GCCGCGGTAC TGGGAAGACC AGTTCCACAA CGACATGAAA 
TCGCTAAATA TTCTCCCGCC AGATGTTTTA ACTCGCGTTT CGGAGTACGT GCCACAAATC 
GTCACCTTTA TTCAAAAGAT TATCGACAAT GGCTTGGCCT ATGCAGCCAA CAACTCCGTT 
TATTTCGATG TAAATGGCTT CGACAAGAGG GAAAAGCATC ATTATGCTAA GCTGGTGCCA 
GAAGCATATG GCGACACCAA GTCCCTGCAA GAAGGTGAAG GCGATCTGTC CATCGCAGAG 
GATCGTCTCT CCGAGAAGCG TTCTGCCAAT GACTTTGCCT TGTGGAAGGC GAGCAAAGCC 
GGCGAACCCT GGTGGGATAG CCCTTGGGGC AAGGGCCGTC CAGGCTGGCA CATTGAATGC 
TCGGCCATGG CCTCGGATAT CTTTGGGCCC ACGTTTGATA TACACACTGG CGGTGTGGAC 
TTAAAGTTCC CTCATCACGA CAACGAGCTT GCCCAATCGG AGGCCGCTTT TAATGAGTCG 
GAGTGGGTTA AGTACTTCCT GCACACGGGT CACCTCACCA TAGCTGGCTG CAAGATGTCC 
AAGTCGCTGA AGAACTTTGT CACCATCCAG GAAGCTCTTA AAAAGCATTC CGCCACCCAG 
CTGCGACTGG CGTTCCTGCT ACACTCGTGG AAGGATACTC TTGACTACTC AGAAAACACC 
ATGGAAATGG CCACCCAATA CGAAAAGTTC CTCAATGAGT TCTTCCTGAA TGTCAAGGAT 
CTGACGCGGC ATGTGCTCTC CGAAGAGCCC CGCCGGCAGT TTGATGCCTG GACAGAAGTT 
GAGGCGGCTT TGCAGAAGAA ATTCTCAAAT GCTCAGGTCC AAGTACACGC CAGCTTGTGT 
GACAACATTG ATACTCGCAG CGCCCTTGAC GCCATTCGAG AGCTGGTATC CGTGTCCAAT 
GTCTACATTC GCGACAATAA GACTAGACTC AACAGTCTGC TGCTCCGAAA CGTAGCCACA 
TATATCACCG ATCTGTTGCA CGTGTTTGGC GCAATATCTG GTCCTCGCGG TGGCATTGGC 
TTCCCCGTTA GTGGTGGCTC GGGAGCACAA GCTGCCGGCG CGGATTTAGA AACTACGGTT 
TTGCCTTACG TTCAATCGCT GGCTGAATTC CGCTACTTGG TGCGGGAGCA AGCCAAAACT 
CTGAAGGCCT TCGACATCCT GAAGCTCTGC GATGATCTAC GCGACAATGT GCTGCCCAAT 
TTGGGCGTGC GACTGGAGGA CAAGGATATT GGAAAGTATG CCGTAAAGCT AGTCGACCGC 
GACTCACTTT TACGAGAACG GGAGGCAAAG CTGGCCGCCG AAGCGGAGAA GGCAGCAGAA 
AAGGAGCGCA AGAAGCAGGC GGCTGCGGAG GCTGCAGCTG CGAAAGAAGC CCAGCGACGT 
GTGAATCCCA AGGAAATGTT CCTGGCCGAA ACCGAAAAGT ACTCAGCGTT CGACGAAAAT 
GGCCTTCCCA CTCACGACAA GGAGGGCAAA GAAGTCAGTA AAGGACAGAT CAAGAAGCTG 
CAGAAACTCC AACAGCAGCA GGAACAGCGT TACAATGAGT ATTTGGCATC AATTGAGAAA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611087.1
CDS	90..2315
Translation	MSKRGQPAWQAPEAVDRPKLKLFNSLTRQKEDFVPLDGNNVTWYSCGPTVYDASHMGHARSYISFDILRRILSDYFGYNIHYVMNITDIDDKIIRRARQNHLFDEYAAEAQKLPLDELLGQQKEVLQRFQDTCAKNTDPDKKIMLDKTLQRMNDAVEALTKAVGKGDEREISEKRLLYLNEAKDPISDWLDSLKGAQINDNAVFEALPRYWEDQFHNDMKSLNILPPDVLTRVSEYVPQIVTFIQKIIDNGLAYAANNSVYFDVNGFDKREKHHYAKLVPEAYGDTKSLQEGEGDLSIAEDRLSEKRSANDFALWKASKAGEPWWDSPWGKGRPGWHIECSAMASDIFGPTFDIHTGGVDLKFPHHDNELAQSEAAFNESEWVKYFLHTGHLTIAGCKMSKSLKNFVTIQEALKKHSATQLRLAFLLHSWKDTLDYSENTMEMATQYEKFLNEFFLNVKDLTRHVLSEEPRRQFDAWTEVEAALQKKFSNAQVQVHASLCDNIDTRSALDAIRELVSVSNVYIRDNKTRLNSLLLRNVATYITDLLHVFGAISGPRGGIGFPVSGGSGAQAAGADLETTVLPYVQSLAEFRYLVREQAKTLKAFDILKLCDDLRDNVLPNLGVRLEDKDIGKYAVKLVDRDSLLREREAKLAAEAEKAAEKERKKQAAAEAAAAKEAQRRVNPKEMFLAETEKYSAFDENGLPTHDKEGKEVSKGQIKKLQKLQQQQEQRYNEYLASIEKA

Target ORF information:

RefSeq Version	NM_137243.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cysteinyl-tRNA synthetase (CysRS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137243.3

ORF Insert Sequence:

ATGTCGAAAC GTGGGCAACC GGCGTGGCAA GCTCCCGAGG CGGTGGATCG GCCGAAACTG 
AAGCTGTTCA ACAGCCTGAC GCGACAGAAG GAGGACTTCG TGCCGCTGGA CGGCAATAAT 
GTAACGTGGT ATAGCTGCGG ACCCACCGTC TACGATGCCT CACACATGGG GCATGCTAGA 
TCTTACATTT CCTTCGACAT TCTGCGGCGC ATTCTGTCCG ACTACTTTGG CTACAACATT 
CACTATGTGA TGAACATTAC GGACATCGAC GACAAGATCA TAAGGCGTGC GCGGCAGAAT 
CATCTCTTCG ACGAGTACGC TGCTGAGGCG CAAAAGCTGC CACTGGATGA GCTGCTGGGT 
CAGCAAAAGG AGGTACTGCA GCGGTTTCAG GATACATGCG CCAAGAATAC AGATCCCGAC 
AAGAAGATTA TGCTCGACAA GACACTGCAG CGGATGAACG ATGCTGTGGA GGCGCTGACC 
AAGGCCGTTG GCAAGGGTGA TGAGCGGGAG ATATCCGAGA AGCGGCTGCT TTACCTAAAC 
GAAGCCAAGG ATCCAATTTC CGACTGGTTG GACTCGCTAA AGGGTGCTCA GATCAATGAT 
AATGCAGTAT TTGAGGCACT GCCGCGGTAC TGGGAAGACC AGTTCCACAA CGACATGAAA 
TCGCTAAATA TTCTCCCGCC AGATGTTTTA ACTCGCGTTT CGGAGTACGT GCCACAAATC 
GTCACCTTTA TTCAAAAGAT TATCGACAAT GGCTTGGCCT ATGCAGCCAA CAACTCCGTT 
TATTTCGATG TAAATGGCTT CGACAAGAGG GAAAAGCATC ATTATGCTAA GCTGGTGCCA 
GAAGCATATG GCGACACCAA GTCCCTGCAA GAAGGTGAAG GCGATCTGTC CATCGCAGAG 
GATCGTCTCT CCGAGAAGCG TTCTGCCAAT GACTTTGCCT TGTGGAAGGC GAGCAAAGCC 
GGCGAACCCT GGTGGGATAG CCCTTGGGGC AAGGGCCGTC CAGGCTGGCA CATTGAATGC 
TCGGCCATGG CCTCGGATAT CTTTGGGCCC ACGTTTGATA TACACACTGG CGGTGTGGAC 
TTAAAGTTCC CTCATCACGA CAACGAGCTT GCCCAATCGG AGGCCGCTTT TAATGAGTCG 
GAGTGGGTTA AGTACTTCCT GCACACGGGT CACCTCACCA TAGCTGGCTG CAAGATGTCC 
AAGTCGCTGA AGAACTTTGT CACCATCCAG GAAGCTCTTA AAAAGCATTC CGCCACCCAG 
CTGCGACTGG CGTTCCTGCT ACACTCGTGG AAGGATACTC TTGACTACTC AGAAAACACC 
ATGGAAATGG CCACCCAATA CGAAAAGTTC CTCAATGAGT TCTTCCTGAA TGTCAAGGAT 
CTGACGCGGC ATGTGCTCTC CGAAGAGCCC CGCCGGCAGT TTGATGCCTG GACAGAAGTT 
GAGGCGGCTT TGCAGAAGAA ATTCTCAAAT GCTCAGGTCC AAGTACACGC CAGCTTGTGT 
GACAACATTG ATACTCGCAG CGCCCTTGAC GCCATTCGAG AGCTGGTATC CGTGTCCAAT 
GTCTACATTC GCGACAATAA GACTAGACTC AACAGTCTGC TGCTCCGAAA CGTAGCCACA 
TATATCACCG ATCTGTTGCA CGTGTTTGGC GCAATATCTG GTCCTCGCGG TGGCATTGGC 
TTCCCCGTTA GTGGTGGCTC GGGAGCACAA GCTGCCGGCG CGGATTTAGA AACTACGGTT 
TTGCCTTACG TTCAATCGCT GGCTGAATTC CGCTACTTGG TGCGGGAGCA AGCCAAAACT 
CTGAAGGCCT TCGACATCCT GAAGCTCTGC GATGATCTAC GCGACAATGT GCTGCCCAAT 
TTGGGCGTGC GACTGGAGGA CAAGGATATT GGAAAGTATG CCGTAAAGCT AGTCGACCGC 
GACTCACTTT TACGAGAACG GGAGGCAAAG CTGGCCGCCG AAGCGGAGAA GGCAGCAGAA 
AAGGAGCGCA AGAAGCAGGC GGCTGCGGAG GCTGCAGCTG CGAAAGAAGC CCAGCGACGT 
GTGAATCCCA AGGAAATGTT CCTGGCCGAA ACCGAAAAGT ACTCAGCGTT CGACGAAAAT 
GGCCTTCCCA CTCACGACAA GGAGGGCAAA GAAGTCAGTA AAGGACAGAT CAAGAAGCTG 
CAGAAACTCC AACAGCAGCA GGAACAGCGT TACAATGAGT ATTTGGCATC AATTGAGAAA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CysRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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