Diap2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Diap2
Entrez Gene ID	36748
Full Name	Death-associated inhibitor of apoptosis 2
Synonyms	CG8293,D-IAP2,D-iap2,DIAP,DIAP-2,DIAP2,DIAPII,DIHA,Diap,Diap-2,Diha,Dmel\CG8293,IAP,IAP2,Iap2,Ilp,dIAP,dIAP2,dILP,diap2
General protein information	Preferred Names Death-associated inhibitor of apoptosis 2 Names CG8293 gene product from transcript CG8293-RB CG8293-PA CG8293-PB Diap2-PA Diap2-PB IAP-like protein drosophila inhibitor of apoptosis 2 drosophila melanogaster inhibitor of apoptosis 2 inhibitor of apoptosis 2 inhibitor of apoptosis protein 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52D2-52D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_176182.2	NP_788362.1	Death-associated inhibitor of apoptosis 2, isoform B
NM_057779.5	NP_477127.1	Death-associated inhibitor of apoptosis 2, isoform A

Pathways from BioSystems

Homologies

	BIRC2	XP_002799824.1
Bos taurus (cattle)	LOC539314	XP_005215803.1
Gallus gallus (chicken)	BIRC2	NP_001007823.1
Canis lupus familiaris (dog)	BIRC2	NP_001041488.1
Mus musculus (house mouse)	Birc2	NP_031491.2
Danio rerio (zebrafish)	birc2	NP_919376.1
Pan troglodytes (chimpanzee)	BIRC2	XP_001152534.1
Rattus norvegicus (Norway rat)	Birc2	NP_068520.2
Xenopus tropicalis (tropical clawed frog)	birc2	NP_001005449.1
Homo sapiens (human)	BIRC2	NP_001243092.1
Drosophila melanogaster (fruit fly)	Iap2	NP_788362.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cleaved protein retains the ability to interact with immune deficiency pathway signal transduction
Title: Independent Proteolytic Activities Control the Stability and Size of Drosophila Inhibitor of Apoptosis 2 Protein.

The authors show that stimulation of the Drosophila pattern recognition protein PGRP-LCx induces DIAP2-dependent polyubiquitylation of the initiator caspase DREDD.
Title: Ubiquitylation of the initiator caspase DREDD is required for innate immune signalling.

Because DIAP2 efficiently interacts with drICE, our data suggest that DIAP2 controls drICE in its apoptotic and nonapoptotic roles.
Title: DIAP2 functions as a mechanism-based regulator of drICE that contributes to the caspase activity threshold in living cells.

Required to sustain antimicrobial peptide gene expression; inactivation results in impaired microbial resistance.
Title: Iap2 is required for a sustained response in the Drosophila Imd pathway.

diap2 may identify a point of convergence between apoptosis and immune signaling pathways
Title: The Drosophila inhibitor of apoptosis (IAP) DIAP2 is dispensable for cell survival, required for the innate immune response to gram-negative bacterial infection, and can be negatively regulated by the reaper/hid/grim family of IAP-binding apoptosis inducers.

The following Diap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Diap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10129	NM_057779.5 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 2 (Diap2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10129	NM_176182.2 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 2 (Diap2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Diap2 ( NM_176182.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788362.1 Drosophila melanogaster Death-associated inhibitor of apoptosis 2 (Diap2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10129
Clone ID Related Accession (Same CDS sequence)	NM_176182.2 , NM_057779.5
Accession Version	NM_176182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_176182.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC TGGGCATGGA GCTGGAGAGC GTTCGCCTGG CGACATTTGG GGAATGGCCC 
CTGAATGCCC CAGTTTCCGC GGAGGATCTG GTCGCCAATG GTTTCTTTGC CACGGGAAAC 
TGGCTGGAGG CCGAGTGCCA TTTCTGCCAC GTGCGCATCG ACCGCTGGGA ATACGGCGAT 
CAAGTGGCGG AGCGCCATCG CCGCTCCTCG CCCATCTGCT CCATGGTTCT GGCTCCCAAT 
CACTGCGGCA ATGTTCCCAG GAGCCAGGAG AGCGACAACG AGGGAAACAG CGTAGTGGAC 
AGCCCGGAGT CCTGCTCTTG TCCCGATCTC TTGTTGGAGG CCAATCGCTT GGTAACTTTC 
AAGGACTGGC CGAATCCCAA CATCACGCCG CAGGCTCTGG CAAAGGCAGG TTTCTACTAC 
CTGAACCGTC TGGATCACGT GAAGTGTGTT TGGTGCAACG GAGTGATTGC CAAGTGGGAG 
AAGAACGACA ATGCCTTTGA AGAGCACAAG CGCTTTTTTC CCCAATGTCC TCGTGTGCAA 
ATGGGCCCCC TTATAGAGTT TGCCACCGGG AAGAACCTGG ATGAGCTGGG CATCCAGCCC 
ACCACTCTGC CACTGCGTCC CAAGTACGCC TGCGTGGACG CCAGATTGAG AACCTTCACC 
GATTGGCCCA TTTCCAATAT TCAGCCCGCT TCGGCTTTGG CGCAGGCTGG CCTATATTAT 
CAGAAAATAG GCGACCAGGT GCGCTGTTTC CACTGCAACA TTGGATTGCG CTCCTGGCAG 
AAGGAGGACG AGCCGTGGTT CGAACACGCC AAGTGGTCGC CAAAGTGTCA GTTCGTTTTG 
CTGGCCAAGG GTCCAGCCTA TGTAAGCGAA GTGCTCGCGA CAACAGCTGC CAACGCCAGT 
TCCCAGCCAG CAACTGCTCC GGCTCCAACG CTCCAAGCAG ACGTACTGAT GGATGAAGCT 
CCGGCCAAGG AGGCCCTAGC CTTGGGTATC GATGGAGGAG TTGTGCGCAA TGCCATCCAG 
CGCAAGCTCT TGAGTTCCGG CTGCGCTTTC TCTACGTTGG ACGAACTATT GCATGACATC 
TTTGACGATG CGGGCGCAGG AGCTGCACTG GAGGTGCGCG AGCCGCCGGA GCCAAGTGCT 
CCTTTTATTG AACCATGTCA GGCCACCACC AGCAAGGCAG CATCTGTGCC AATACCGGTG 
GCCGATTCCA TCCCAGCCAA ACCACAGGCA GCTGAAGCAG TGGCGAATAT ATCGAAAATC 
ACAGACGAAA TACAAAAGAT GTCGGTGGCC ACGCCGAACG GAAACCTATC GCTGGAGGAG 
GAAAACCGCC AGCTAAAGGA TGCACGCTTA TGCAAGGTAT GCTTGGACGA GGAGGTTGGC 
GTAGTGTTCC TGCCCTGTGG CCACTTGGCC ACCTGCAATC AGTGCGCCCC CAGTGTAGCC 
AATTGTCCCA TGTGCCGCGC AGACATCAAG GGATTCGTGC GCACGTTCCT TTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788362.1
CDS	39..1535
Translation	MTELGMELESVRLATFGEWPLNAPVSAEDLVANGFFATGNWLEAECHFCHVRIDRWEYGDQVAERHRRSSPICSMVLAPNHCGNVPRSQESDNEGNSVVDSPESCSCPDLLLEANRLVTFKDWPNPNITPQALAKAGFYYLNRLDHVKCVWCNGVIAKWEKNDNAFEEHKRFFPQCPRVQMGPLIEFATGKNLDELGIQPTTLPLRPKYACVDARLRTFTDWPISNIQPASALAQAGLYYQKIGDQVRCFHCNIGLRSWQKEDEPWFEHAKWSPKCQFVLLAKGPAYVSEVLATTAANASSQPATAPAPTLQADVLMDEAPAKEALALGIDGGVVRNAIQRKLLSSGCAFSTLDELLHDIFDDAGAGAALEVREPPEPSAPFIEPCQATTSKAASVPIPVADSIPAKPQAAEAVANISKITDEIQKMSVATPNGNLSLEEENRQLKDARLCKVCLDEEVGVVFLPCGHLATCNQCAPSVANCPMCRADIKGFVRTFLS

Target ORF information:

RefSeq Version	NM_176182.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 2 (Diap2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176182.2

ORF Insert Sequence:

ATGACGGAGC TGGGCATGGA GCTGGAGAGC GTTCGCCTGG CGACATTTGG GGAATGGCCC 
CTGAATGCCC CAGTTTCCGC GGAGGATCTG GTCGCCAATG GTTTCTTTGC CACGGGAAAC 
TGGCTGGAGG CCGAGTGCCA TTTCTGCCAC GTGCGCATCG ACCGCTGGGA ATACGGCGAT 
CAAGTGGCGG AGCGCCATCG CCGCTCCTCG CCCATCTGCT CCATGGTTCT GGCTCCCAAT 
CACTGCGGCA ATGTTCCCAG GAGCCAGGAG AGCGACAACG AGGGAAACAG CGTAGTGGAC 
AGCCCGGAGT CCTGCTCTTG TCCCGATCTC TTGTTGGAGG CCAATCGCTT GGTAACTTTC 
AAGGACTGGC CGAATCCCAA CATCACGCCG CAGGCTCTGG CAAAGGCAGG TTTCTACTAC 
CTGAACCGTC TGGATCACGT GAAGTGTGTT TGGTGCAACG GAGTGATTGC CAAGTGGGAG 
AAGAACGACA ATGCCTTTGA AGAGCACAAG CGCTTTTTTC CCCAATGTCC TCGTGTGCAA 
ATGGGCCCCC TTATAGAGTT TGCCACCGGG AAGAACCTGG ATGAGCTGGG CATCCAGCCC 
ACCACTCTGC CACTGCGTCC CAAGTACGCC TGCGTGGACG CCAGATTGAG AACCTTCACC 
GATTGGCCCA TTTCCAATAT TCAGCCCGCT TCGGCTTTGG CGCAGGCTGG CCTATATTAT 
CAGAAAATAG GCGACCAGGT GCGCTGTTTC CACTGCAACA TTGGATTGCG CTCCTGGCAG 
AAGGAGGACG AGCCGTGGTT CGAACACGCC AAGTGGTCGC CAAAGTGTCA GTTCGTTTTG 
CTGGCCAAGG GTCCAGCCTA TGTAAGCGAA GTGCTCGCGA CAACAGCTGC CAACGCCAGT 
TCCCAGCCAG CAACTGCTCC GGCTCCAACG CTCCAAGCAG ACGTACTGAT GGATGAAGCT 
CCGGCCAAGG AGGCCCTAGC CTTGGGTATC GATGGAGGAG TTGTGCGCAA TGCCATCCAG 
CGCAAGCTCT TGAGTTCCGG CTGCGCTTTC TCTACGTTGG ACGAACTATT GCATGACATC 
TTTGACGATG CGGGCGCAGG AGCTGCACTG GAGGTGCGCG AGCCGCCGGA GCCAAGTGCT 
CCTTTTATTG AACCATGTCA GGCCACCACC AGCAAGGCAG CATCTGTGCC AATACCGGTG 
GCCGATTCCA TCCCAGCCAA ACCACAGGCA GCTGAAGCAG TGGCGAATAT ATCGAAAATC 
ACAGACGAAA TACAAAAGAT GTCGGTGGCC ACGCCGAACG GAAACCTATC GCTGGAGGAG 
GAAAACCGCC AGCTAAAGGA TGCACGCTTA TGCAAGGTAT GCTTGGACGA GGAGGTTGGC 
GTAGTGTTCC TGCCCTGTGG CCACTTGGCC ACCTGCAATC AGTGCGCCCC CAGTGTAGCC 
AATTGTCCCA TGTGCCGCGC AGACATCAAG GGATTCGTGC GCACGTTCCT TTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diap2 ( NM_057779.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477127.1 Drosophila melanogaster Death-associated inhibitor of apoptosis 2 (Diap2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10129
Clone ID Related Accession (Same CDS sequence)	NM_176182.2 , NM_057779.5
Accession Version	NM_057779.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057779.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC TGGGCATGGA GCTGGAGAGC GTTCGCCTGG CGACATTTGG GGAATGGCCC 
CTGAATGCCC CAGTTTCCGC GGAGGATCTG GTCGCCAATG GTTTCTTTGC CACGGGAAAC 
TGGCTGGAGG CCGAGTGCCA TTTCTGCCAC GTGCGCATCG ACCGCTGGGA ATACGGCGAT 
CAAGTGGCGG AGCGCCATCG CCGCTCCTCG CCCATCTGCT CCATGGTTCT GGCTCCCAAT 
CACTGCGGCA ATGTTCCCAG GAGCCAGGAG AGCGACAACG AGGGAAACAG CGTAGTGGAC 
AGCCCGGAGT CCTGCTCTTG TCCCGATCTC TTGTTGGAGG CCAATCGCTT GGTAACTTTC 
AAGGACTGGC CGAATCCCAA CATCACGCCG CAGGCTCTGG CAAAGGCAGG TTTCTACTAC 
CTGAACCGTC TGGATCACGT GAAGTGTGTT TGGTGCAACG GAGTGATTGC CAAGTGGGAG 
AAGAACGACA ATGCCTTTGA AGAGCACAAG CGCTTTTTTC CCCAATGTCC TCGTGTGCAA 
ATGGGCCCCC TTATAGAGTT TGCCACCGGG AAGAACCTGG ATGAGCTGGG CATCCAGCCC 
ACCACTCTGC CACTGCGTCC CAAGTACGCC TGCGTGGACG CCAGATTGAG AACCTTCACC 
GATTGGCCCA TTTCCAATAT TCAGCCCGCT TCGGCTTTGG CGCAGGCTGG CCTATATTAT 
CAGAAAATAG GCGACCAGGT GCGCTGTTTC CACTGCAACA TTGGATTGCG CTCCTGGCAG 
AAGGAGGACG AGCCGTGGTT CGAACACGCC AAGTGGTCGC CAAAGTGTCA GTTCGTTTTG 
CTGGCCAAGG GTCCAGCCTA TGTAAGCGAA GTGCTCGCGA CAACAGCTGC CAACGCCAGT 
TCCCAGCCAG CAACTGCTCC GGCTCCAACG CTCCAAGCAG ACGTACTGAT GGATGAAGCT 
CCGGCCAAGG AGGCCCTAGC CTTGGGTATC GATGGAGGAG TTGTGCGCAA TGCCATCCAG 
CGCAAGCTCT TGAGTTCCGG CTGCGCTTTC TCTACGTTGG ACGAACTATT GCATGACATC 
TTTGACGATG CGGGCGCAGG AGCTGCACTG GAGGTGCGCG AGCCGCCGGA GCCAAGTGCT 
CCTTTTATTG AACCATGTCA GGCCACCACC AGCAAGGCAG CATCTGTGCC AATACCGGTG 
GCCGATTCCA TCCCAGCCAA ACCACAGGCA GCTGAAGCAG TGGCGAATAT ATCGAAAATC 
ACAGACGAAA TACAAAAGAT GTCGGTGGCC ACGCCGAACG GAAACCTATC GCTGGAGGAG 
GAAAACCGCC AGCTAAAGGA TGCACGCTTA TGCAAGGTAT GCTTGGACGA GGAGGTTGGC 
GTAGTGTTCC TGCCCTGTGG CCACTTGGCC ACCTGCAATC AGTGCGCCCC CAGTGTAGCC 
AATTGTCCCA TGTGCCGCGC AGACATCAAG GGATTCGTGC GCACGTTCCT TTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477127.1
CDS	148..1644
Translation	MTELGMELESVRLATFGEWPLNAPVSAEDLVANGFFATGNWLEAECHFCHVRIDRWEYGDQVAERHRRSSPICSMVLAPNHCGNVPRSQESDNEGNSVVDSPESCSCPDLLLEANRLVTFKDWPNPNITPQALAKAGFYYLNRLDHVKCVWCNGVIAKWEKNDNAFEEHKRFFPQCPRVQMGPLIEFATGKNLDELGIQPTTLPLRPKYACVDARLRTFTDWPISNIQPASALAQAGLYYQKIGDQVRCFHCNIGLRSWQKEDEPWFEHAKWSPKCQFVLLAKGPAYVSEVLATTAANASSQPATAPAPTLQADVLMDEAPAKEALALGIDGGVVRNAIQRKLLSSGCAFSTLDELLHDIFDDAGAGAALEVREPPEPSAPFIEPCQATTSKAASVPIPVADSIPAKPQAAEAVANISKITDEIQKMSVATPNGNLSLEEENRQLKDARLCKVCLDEEVGVVFLPCGHLATCNQCAPSVANCPMCRADIKGFVRTFLS

Target ORF information:

RefSeq Version	NM_057779.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 2 (Diap2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057779.5

ORF Insert Sequence:

ATGACGGAGC TGGGCATGGA GCTGGAGAGC GTTCGCCTGG CGACATTTGG GGAATGGCCC 
CTGAATGCCC CAGTTTCCGC GGAGGATCTG GTCGCCAATG GTTTCTTTGC CACGGGAAAC 
TGGCTGGAGG CCGAGTGCCA TTTCTGCCAC GTGCGCATCG ACCGCTGGGA ATACGGCGAT 
CAAGTGGCGG AGCGCCATCG CCGCTCCTCG CCCATCTGCT CCATGGTTCT GGCTCCCAAT 
CACTGCGGCA ATGTTCCCAG GAGCCAGGAG AGCGACAACG AGGGAAACAG CGTAGTGGAC 
AGCCCGGAGT CCTGCTCTTG TCCCGATCTC TTGTTGGAGG CCAATCGCTT GGTAACTTTC 
AAGGACTGGC CGAATCCCAA CATCACGCCG CAGGCTCTGG CAAAGGCAGG TTTCTACTAC 
CTGAACCGTC TGGATCACGT GAAGTGTGTT TGGTGCAACG GAGTGATTGC CAAGTGGGAG 
AAGAACGACA ATGCCTTTGA AGAGCACAAG CGCTTTTTTC CCCAATGTCC TCGTGTGCAA 
ATGGGCCCCC TTATAGAGTT TGCCACCGGG AAGAACCTGG ATGAGCTGGG CATCCAGCCC 
ACCACTCTGC CACTGCGTCC CAAGTACGCC TGCGTGGACG CCAGATTGAG AACCTTCACC 
GATTGGCCCA TTTCCAATAT TCAGCCCGCT TCGGCTTTGG CGCAGGCTGG CCTATATTAT 
CAGAAAATAG GCGACCAGGT GCGCTGTTTC CACTGCAACA TTGGATTGCG CTCCTGGCAG 
AAGGAGGACG AGCCGTGGTT CGAACACGCC AAGTGGTCGC CAAAGTGTCA GTTCGTTTTG 
CTGGCCAAGG GTCCAGCCTA TGTAAGCGAA GTGCTCGCGA CAACAGCTGC CAACGCCAGT 
TCCCAGCCAG CAACTGCTCC GGCTCCAACG CTCCAAGCAG ACGTACTGAT GGATGAAGCT 
CCGGCCAAGG AGGCCCTAGC CTTGGGTATC GATGGAGGAG TTGTGCGCAA TGCCATCCAG 
CGCAAGCTCT TGAGTTCCGG CTGCGCTTTC TCTACGTTGG ACGAACTATT GCATGACATC 
TTTGACGATG CGGGCGCAGG AGCTGCACTG GAGGTGCGCG AGCCGCCGGA GCCAAGTGCT 
CCTTTTATTG AACCATGTCA GGCCACCACC AGCAAGGCAG CATCTGTGCC AATACCGGTG 
GCCGATTCCA TCCCAGCCAA ACCACAGGCA GCTGAAGCAG TGGCGAATAT ATCGAAAATC 
ACAGACGAAA TACAAAAGAT GTCGGTGGCC ACGCCGAACG GAAACCTATC GCTGGAGGAG 
GAAAACCGCC AGCTAAAGGA TGCACGCTTA TGCAAGGTAT GCTTGGACGA GGAGGTTGGC 
GTAGTGTTCC TGCCCTGTGG CCACTTGGCC ACCTGCAATC AGTGCGCCCC CAGTGTAGCC 
AATTGTCCCA TGTGCCGCGC AGACATCAAG GGATTCGTGC GCACGTTCCT TTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Diap2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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