CG8249 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8249
Entrez Gene ID	36742
Full Name	CG8249 gene product from transcript CG8249-RC
Synonyms	Dmel\CG8249
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52C8-52C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_137216.4	NP_611060.2	uncharacterized protein, isoform A
NM_001259420.2	NP_001246349.1	uncharacterized protein, isoform B
NM_001259421.2	NP_001246350.1	uncharacterized protein, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG8249	NP_001246349.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008721	XP_314829.4
Drosophila melanogaster (fruit fly)	CG4607	NP_727159.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8249 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8249 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10122	NM_001259420.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8249), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10122	NM_137216.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8249), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10122	NM_001259421.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8249), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8249 ( NM_137216.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611060.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8249), mRNA.

Price & Availability↑

CloneID	OFa10122
Clone ID Related Accession (Same CDS sequence)	NM_137216.4 , NM_001259420.2 , NM_001259421.2
Accession Version	NM_137216.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137216.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAGC AGCATCAGAA CCTCAATCAA AATGGCAGCG AATCCAATGG TAAGCAGGCC 
AGGATCGTTT CGGGACTTGA TCGTCTGCTG AAGACTCCTC CGCCTGGCGA ACAGACAAGA 
GCAGTGCGGC GACAAGTGAT CGCTGTTATC CTGGCCAACG TGGGTGTCTT CTCAACGGGA 
ATGACCCTGG CCATGCCCAC CGCCACTCTG CATCAGCTGA AGGACACCAC GGAGCCAGTG 
CATCTGAACG ATTCGCAGGC TTCCTGGTTC GCATCGGTAA ACGCTCTGTC CGCTCCTATA 
GGCGGCTTGC TGTCCGGTTT CTTGCTTGAC AGGATTGGAA GGAAGAAATC CCTGATAGTG 
CTCAATGTAC TGATTATTCT TGCTTGGATT CTGCTGGCCA CACCCAGTGA GAGCGACCAA 
AATGCCTTCT TTTGGCAACT AATTGTCTCA CGATTTATGC TGGGTGTTGG TATGGGACTG 
GCCAGTGCGC CACCAGGAGT CTATGCAGCC GAGATCAGTG TGCCCAAGAC GAGGGGCAGT 
CTTATACTGG GCACATCCAT TTCAGTGGCC GGTGGCATAA CGATTCTCTA CGGAATTGGT 
TACTGCATCC GCGATGATTT CCGACTGATT GCCCTCATCT GCTGTGGCTA CCAGCTGGTG 
GCCCTGCTCT GCGTGCTGCC ACTTCCGGAA AGTCATTGCT GGCTTCTGTC CAAGAAGCGT 
GTGACGGAGG CGAAGAGATC ACTTAACTAC TTCCGTGGAT TCAACAAATC GGATGAAATC 
ACCCATCCCC AAGTGCTCGA AGAGTTCCAG CTTCTGCAGA AATCCCTGCA GCAAAGGAAC 
ACCGCGGTGA AGGAGTCCTT TTGGCGTAAT CTGCACGAAC CGGAGGTCTA CAAGCCATTG 
GTCATCCTGA TGAGCTTGTT CGCCTTCCAG CAGCTAACCG GAATCTTTGT GGTCATCGTC 
TTCGCCGTGC AAATTTCCCA AGAGGCGGGC ATCGAAATCG ATCCCTTCAT GTGCGCCGTG 
CTCATTGGTT TGGCTCGCTT GATCACCACA TGCCCCATGG GATACATCCT GGAGTGGTGG 
GGTCGCCGAA GGGCAGGGAT TATCTCCACT TTGGGCATGT CCGTGTGCAT GTTCCTTTTG 
GCGGGACATA GCCAAATCGA GATCCTTAAG GAAGTCCCAT ATCTCCCGGT GGTGGCCATT 
GTGGGCTTCA TTGTGCTGAG CACTCTAGGT CTTTACACCC TGCCCTTCTT TATGATCTCC 
GAGCTTTTTC CGCAAAAGGT TCGAGGGCCC GCTTCAGGAC TCACCGTGGC CGTGGGCATG 
TTCATTTCGT TCGTGGTCTT GAAGACCTAT CCCGGCATCA AGGAATACCT GGGAATGTCG 
AATTGCTTCA TCATTTTTGG GGTCATGGCT CTGTTTGCCC TGATCTTCGT TTATCTCGCC 
CTCCCAGAGA CGCGTCGTCG AACGCTCTTG GAGATCGAGG AGCAGTTCCG ATCGGGTCGC 
AGTAAGTCTC AAAACCAGGC TGATGTTGAA ATGAAGGAGG TTTTTGTTCG AAAGCCCGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611060.2
CDS	260..1825
Translation	MDEQHQNLNQNGSESNGKQARIVSGLDRLLKTPPPGEQTRAVRRQVIAVILANVGVFSTGMTLAMPTATLHQLKDTTEPVHLNDSQASWFASVNALSAPIGGLLSGFLLDRIGRKKSLIVLNVLIILAWILLATPSESDQNAFFWQLIVSRFMLGVGMGLASAPPGVYAAEISVPKTRGSLILGTSISVAGGITILYGIGYCIRDDFRLIALICCGYQLVALLCVLPLPESHCWLLSKKRVTEAKRSLNYFRGFNKSDEITHPQVLEEFQLLQKSLQQRNTAVKESFWRNLHEPEVYKPLVILMSLFAFQQLTGIFVVIVFAVQISQEAGIEIDPFMCAVLIGLARLITTCPMGYILEWWGRRRAGIISTLGMSVCMFLLAGHSQIEILKEVPYLPVVAIVGFIVLSTLGLYTLPFFMISELFPQKVRGPASGLTVAVGMFISFVVLKTYPGIKEYLGMSNCFIIFGVMALFALIFVYLALPETRRRTLLEIEEQFRSGRSKSQNQADVEMKEVFVRKPEE

Target ORF information:

RefSeq Version	NM_137216.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8249), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137216.4

ORF Insert Sequence:

ATGGATGAGC AGCATCAGAA CCTCAATCAA AATGGCAGCG AATCCAATGG TAAGCAGGCC 
AGGATCGTTT CGGGACTTGA TCGTCTGCTG AAGACTCCTC CGCCTGGCGA ACAGACAAGA 
GCAGTGCGGC GACAAGTGAT CGCTGTTATC CTGGCCAACG TGGGTGTCTT CTCAACGGGA 
ATGACCCTGG CCATGCCCAC CGCCACTCTG CATCAGCTGA AGGACACCAC GGAGCCAGTG 
CATCTGAACG ATTCGCAGGC TTCCTGGTTC GCATCGGTAA ACGCTCTGTC CGCTCCTATA 
GGCGGCTTGC TGTCCGGTTT CTTGCTTGAC AGGATTGGAA GGAAGAAATC CCTGATAGTG 
CTCAATGTAC TGATTATTCT TGCTTGGATT CTGCTGGCCA CACCCAGTGA GAGCGACCAA 
AATGCCTTCT TTTGGCAACT AATTGTCTCA CGATTTATGC TGGGTGTTGG TATGGGACTG 
GCCAGTGCGC CACCAGGAGT CTATGCAGCC GAGATCAGTG TGCCCAAGAC GAGGGGCAGT 
CTTATACTGG GCACATCCAT TTCAGTGGCC GGTGGCATAA CGATTCTCTA CGGAATTGGT 
TACTGCATCC GCGATGATTT CCGACTGATT GCCCTCATCT GCTGTGGCTA CCAGCTGGTG 
GCCCTGCTCT GCGTGCTGCC ACTTCCGGAA AGTCATTGCT GGCTTCTGTC CAAGAAGCGT 
GTGACGGAGG CGAAGAGATC ACTTAACTAC TTCCGTGGAT TCAACAAATC GGATGAAATC 
ACCCATCCCC AAGTGCTCGA AGAGTTCCAG CTTCTGCAGA AATCCCTGCA GCAAAGGAAC 
ACCGCGGTGA AGGAGTCCTT TTGGCGTAAT CTGCACGAAC CGGAGGTCTA CAAGCCATTG 
GTCATCCTGA TGAGCTTGTT CGCCTTCCAG CAGCTAACCG GAATCTTTGT GGTCATCGTC 
TTCGCCGTGC AAATTTCCCA AGAGGCGGGC ATCGAAATCG ATCCCTTCAT GTGCGCCGTG 
CTCATTGGTT TGGCTCGCTT GATCACCACA TGCCCCATGG GATACATCCT GGAGTGGTGG 
GGTCGCCGAA GGGCAGGGAT TATCTCCACT TTGGGCATGT CCGTGTGCAT GTTCCTTTTG 
GCGGGACATA GCCAAATCGA GATCCTTAAG GAAGTCCCAT ATCTCCCGGT GGTGGCCATT 
GTGGGCTTCA TTGTGCTGAG CACTCTAGGT CTTTACACCC TGCCCTTCTT TATGATCTCC 
GAGCTTTTTC CGCAAAAGGT TCGAGGGCCC GCTTCAGGAC TCACCGTGGC CGTGGGCATG 
TTCATTTCGT TCGTGGTCTT GAAGACCTAT CCCGGCATCA AGGAATACCT GGGAATGTCG 
AATTGCTTCA TCATTTTTGG GGTCATGGCT CTGTTTGCCC TGATCTTCGT TTATCTCGCC 
CTCCCAGAGA CGCGTCGTCG AACGCTCTTG GAGATCGAGG AGCAGTTCCG ATCGGGTCGC 
AGTAAGTCTC AAAACCAGGC TGATGTTGAA ATGAAGGAGG TTTTTGTTCG AAAGCCCGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8249 ( NM_001259420.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246349.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8249), mRNA.

Price & Availability↑

CloneID	OFa10122
Clone ID Related Accession (Same CDS sequence)	NM_137216.4 , NM_001259420.2 , NM_001259421.2
Accession Version	NM_001259420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259420.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAGC AGCATCAGAA CCTCAATCAA AATGGCAGCG AATCCAATGG TAAGCAGGCC 
AGGATCGTTT CGGGACTTGA TCGTCTGCTG AAGACTCCTC CGCCTGGCGA ACAGACAAGA 
GCAGTGCGGC GACAAGTGAT CGCTGTTATC CTGGCCAACG TGGGTGTCTT CTCAACGGGA 
ATGACCCTGG CCATGCCCAC CGCCACTCTG CATCAGCTGA AGGACACCAC GGAGCCAGTG 
CATCTGAACG ATTCGCAGGC TTCCTGGTTC GCATCGGTAA ACGCTCTGTC CGCTCCTATA 
GGCGGCTTGC TGTCCGGTTT CTTGCTTGAC AGGATTGGAA GGAAGAAATC CCTGATAGTG 
CTCAATGTAC TGATTATTCT TGCTTGGATT CTGCTGGCCA CACCCAGTGA GAGCGACCAA 
AATGCCTTCT TTTGGCAACT AATTGTCTCA CGATTTATGC TGGGTGTTGG TATGGGACTG 
GCCAGTGCGC CACCAGGAGT CTATGCAGCC GAGATCAGTG TGCCCAAGAC GAGGGGCAGT 
CTTATACTGG GCACATCCAT TTCAGTGGCC GGTGGCATAA CGATTCTCTA CGGAATTGGT 
TACTGCATCC GCGATGATTT CCGACTGATT GCCCTCATCT GCTGTGGCTA CCAGCTGGTG 
GCCCTGCTCT GCGTGCTGCC ACTTCCGGAA AGTCATTGCT GGCTTCTGTC CAAGAAGCGT 
GTGACGGAGG CGAAGAGATC ACTTAACTAC TTCCGTGGAT TCAACAAATC GGATGAAATC 
ACCCATCCCC AAGTGCTCGA AGAGTTCCAG CTTCTGCAGA AATCCCTGCA GCAAAGGAAC 
ACCGCGGTGA AGGAGTCCTT TTGGCGTAAT CTGCACGAAC CGGAGGTCTA CAAGCCATTG 
GTCATCCTGA TGAGCTTGTT CGCCTTCCAG CAGCTAACCG GAATCTTTGT GGTCATCGTC 
TTCGCCGTGC AAATTTCCCA AGAGGCGGGC ATCGAAATCG ATCCCTTCAT GTGCGCCGTG 
CTCATTGGTT TGGCTCGCTT GATCACCACA TGCCCCATGG GATACATCCT GGAGTGGTGG 
GGTCGCCGAA GGGCAGGGAT TATCTCCACT TTGGGCATGT CCGTGTGCAT GTTCCTTTTG 
GCGGGACATA GCCAAATCGA GATCCTTAAG GAAGTCCCAT ATCTCCCGGT GGTGGCCATT 
GTGGGCTTCA TTGTGCTGAG CACTCTAGGT CTTTACACCC TGCCCTTCTT TATGATCTCC 
GAGCTTTTTC CGCAAAAGGT TCGAGGGCCC GCTTCAGGAC TCACCGTGGC CGTGGGCATG 
TTCATTTCGT TCGTGGTCTT GAAGACCTAT CCCGGCATCA AGGAATACCT GGGAATGTCG 
AATTGCTTCA TCATTTTTGG GGTCATGGCT CTGTTTGCCC TGATCTTCGT TTATCTCGCC 
CTCCCAGAGA CGCGTCGTCG AACGCTCTTG GAGATCGAGG AGCAGTTCCG ATCGGGTCGC 
AGTAAGTCTC AAAACCAGGC TGATGTTGAA ATGAAGGAGG TTTTTGTTCG AAAGCCCGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246349.1
CDS	409..1974
Translation	MDEQHQNLNQNGSESNGKQARIVSGLDRLLKTPPPGEQTRAVRRQVIAVILANVGVFSTGMTLAMPTATLHQLKDTTEPVHLNDSQASWFASVNALSAPIGGLLSGFLLDRIGRKKSLIVLNVLIILAWILLATPSESDQNAFFWQLIVSRFMLGVGMGLASAPPGVYAAEISVPKTRGSLILGTSISVAGGITILYGIGYCIRDDFRLIALICCGYQLVALLCVLPLPESHCWLLSKKRVTEAKRSLNYFRGFNKSDEITHPQVLEEFQLLQKSLQQRNTAVKESFWRNLHEPEVYKPLVILMSLFAFQQLTGIFVVIVFAVQISQEAGIEIDPFMCAVLIGLARLITTCPMGYILEWWGRRRAGIISTLGMSVCMFLLAGHSQIEILKEVPYLPVVAIVGFIVLSTLGLYTLPFFMISELFPQKVRGPASGLTVAVGMFISFVVLKTYPGIKEYLGMSNCFIIFGVMALFALIFVYLALPETRRRTLLEIEEQFRSGRSKSQNQADVEMKEVFVRKPEE

Target ORF information:

RefSeq Version	NM_001259420.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8249), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259420.2

ORF Insert Sequence:

ATGGATGAGC AGCATCAGAA CCTCAATCAA AATGGCAGCG AATCCAATGG TAAGCAGGCC 
AGGATCGTTT CGGGACTTGA TCGTCTGCTG AAGACTCCTC CGCCTGGCGA ACAGACAAGA 
GCAGTGCGGC GACAAGTGAT CGCTGTTATC CTGGCCAACG TGGGTGTCTT CTCAACGGGA 
ATGACCCTGG CCATGCCCAC CGCCACTCTG CATCAGCTGA AGGACACCAC GGAGCCAGTG 
CATCTGAACG ATTCGCAGGC TTCCTGGTTC GCATCGGTAA ACGCTCTGTC CGCTCCTATA 
GGCGGCTTGC TGTCCGGTTT CTTGCTTGAC AGGATTGGAA GGAAGAAATC CCTGATAGTG 
CTCAATGTAC TGATTATTCT TGCTTGGATT CTGCTGGCCA CACCCAGTGA GAGCGACCAA 
AATGCCTTCT TTTGGCAACT AATTGTCTCA CGATTTATGC TGGGTGTTGG TATGGGACTG 
GCCAGTGCGC CACCAGGAGT CTATGCAGCC GAGATCAGTG TGCCCAAGAC GAGGGGCAGT 
CTTATACTGG GCACATCCAT TTCAGTGGCC GGTGGCATAA CGATTCTCTA CGGAATTGGT 
TACTGCATCC GCGATGATTT CCGACTGATT GCCCTCATCT GCTGTGGCTA CCAGCTGGTG 
GCCCTGCTCT GCGTGCTGCC ACTTCCGGAA AGTCATTGCT GGCTTCTGTC CAAGAAGCGT 
GTGACGGAGG CGAAGAGATC ACTTAACTAC TTCCGTGGAT TCAACAAATC GGATGAAATC 
ACCCATCCCC AAGTGCTCGA AGAGTTCCAG CTTCTGCAGA AATCCCTGCA GCAAAGGAAC 
ACCGCGGTGA AGGAGTCCTT TTGGCGTAAT CTGCACGAAC CGGAGGTCTA CAAGCCATTG 
GTCATCCTGA TGAGCTTGTT CGCCTTCCAG CAGCTAACCG GAATCTTTGT GGTCATCGTC 
TTCGCCGTGC AAATTTCCCA AGAGGCGGGC ATCGAAATCG ATCCCTTCAT GTGCGCCGTG 
CTCATTGGTT TGGCTCGCTT GATCACCACA TGCCCCATGG GATACATCCT GGAGTGGTGG 
GGTCGCCGAA GGGCAGGGAT TATCTCCACT TTGGGCATGT CCGTGTGCAT GTTCCTTTTG 
GCGGGACATA GCCAAATCGA GATCCTTAAG GAAGTCCCAT ATCTCCCGGT GGTGGCCATT 
GTGGGCTTCA TTGTGCTGAG CACTCTAGGT CTTTACACCC TGCCCTTCTT TATGATCTCC 
GAGCTTTTTC CGCAAAAGGT TCGAGGGCCC GCTTCAGGAC TCACCGTGGC CGTGGGCATG 
TTCATTTCGT TCGTGGTCTT GAAGACCTAT CCCGGCATCA AGGAATACCT GGGAATGTCG 
AATTGCTTCA TCATTTTTGG GGTCATGGCT CTGTTTGCCC TGATCTTCGT TTATCTCGCC 
CTCCCAGAGA CGCGTCGTCG AACGCTCTTG GAGATCGAGG AGCAGTTCCG ATCGGGTCGC 
AGTAAGTCTC AAAACCAGGC TGATGTTGAA ATGAAGGAGG TTTTTGTTCG AAAGCCCGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8249 ( NM_001259421.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246350.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG8249), mRNA.

Price & Availability↑

CloneID	OFa10122
Clone ID Related Accession (Same CDS sequence)	NM_137216.4 , NM_001259420.2 , NM_001259421.2
Accession Version	NM_001259421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259421.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAGC AGCATCAGAA CCTCAATCAA AATGGCAGCG AATCCAATGG TAAGCAGGCC 
AGGATCGTTT CGGGACTTGA TCGTCTGCTG AAGACTCCTC CGCCTGGCGA ACAGACAAGA 
GCAGTGCGGC GACAAGTGAT CGCTGTTATC CTGGCCAACG TGGGTGTCTT CTCAACGGGA 
ATGACCCTGG CCATGCCCAC CGCCACTCTG CATCAGCTGA AGGACACCAC GGAGCCAGTG 
CATCTGAACG ATTCGCAGGC TTCCTGGTTC GCATCGGTAA ACGCTCTGTC CGCTCCTATA 
GGCGGCTTGC TGTCCGGTTT CTTGCTTGAC AGGATTGGAA GGAAGAAATC CCTGATAGTG 
CTCAATGTAC TGATTATTCT TGCTTGGATT CTGCTGGCCA CACCCAGTGA GAGCGACCAA 
AATGCCTTCT TTTGGCAACT AATTGTCTCA CGATTTATGC TGGGTGTTGG TATGGGACTG 
GCCAGTGCGC CACCAGGAGT CTATGCAGCC GAGATCAGTG TGCCCAAGAC GAGGGGCAGT 
CTTATACTGG GCACATCCAT TTCAGTGGCC GGTGGCATAA CGATTCTCTA CGGAATTGGT 
TACTGCATCC GCGATGATTT CCGACTGATT GCCCTCATCT GCTGTGGCTA CCAGCTGGTG 
GCCCTGCTCT GCGTGCTGCC ACTTCCGGAA AGTCATTGCT GGCTTCTGTC CAAGAAGCGT 
GTGACGGAGG CGAAGAGATC ACTTAACTAC TTCCGTGGAT TCAACAAATC GGATGAAATC 
ACCCATCCCC AAGTGCTCGA AGAGTTCCAG CTTCTGCAGA AATCCCTGCA GCAAAGGAAC 
ACCGCGGTGA AGGAGTCCTT TTGGCGTAAT CTGCACGAAC CGGAGGTCTA CAAGCCATTG 
GTCATCCTGA TGAGCTTGTT CGCCTTCCAG CAGCTAACCG GAATCTTTGT GGTCATCGTC 
TTCGCCGTGC AAATTTCCCA AGAGGCGGGC ATCGAAATCG ATCCCTTCAT GTGCGCCGTG 
CTCATTGGTT TGGCTCGCTT GATCACCACA TGCCCCATGG GATACATCCT GGAGTGGTGG 
GGTCGCCGAA GGGCAGGGAT TATCTCCACT TTGGGCATGT CCGTGTGCAT GTTCCTTTTG 
GCGGGACATA GCCAAATCGA GATCCTTAAG GAAGTCCCAT ATCTCCCGGT GGTGGCCATT 
GTGGGCTTCA TTGTGCTGAG CACTCTAGGT CTTTACACCC TGCCCTTCTT TATGATCTCC 
GAGCTTTTTC CGCAAAAGGT TCGAGGGCCC GCTTCAGGAC TCACCGTGGC CGTGGGCATG 
TTCATTTCGT TCGTGGTCTT GAAGACCTAT CCCGGCATCA AGGAATACCT GGGAATGTCG 
AATTGCTTCA TCATTTTTGG GGTCATGGCT CTGTTTGCCC TGATCTTCGT TTATCTCGCC 
CTCCCAGAGA CGCGTCGTCG AACGCTCTTG GAGATCGAGG AGCAGTTCCG ATCGGGTCGC 
AGTAAGTCTC AAAACCAGGC TGATGTTGAA ATGAAGGAGG TTTTTGTTCG AAAGCCCGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246350.1
CDS	260..1825
Translation	MDEQHQNLNQNGSESNGKQARIVSGLDRLLKTPPPGEQTRAVRRQVIAVILANVGVFSTGMTLAMPTATLHQLKDTTEPVHLNDSQASWFASVNALSAPIGGLLSGFLLDRIGRKKSLIVLNVLIILAWILLATPSESDQNAFFWQLIVSRFMLGVGMGLASAPPGVYAAEISVPKTRGSLILGTSISVAGGITILYGIGYCIRDDFRLIALICCGYQLVALLCVLPLPESHCWLLSKKRVTEAKRSLNYFRGFNKSDEITHPQVLEEFQLLQKSLQQRNTAVKESFWRNLHEPEVYKPLVILMSLFAFQQLTGIFVVIVFAVQISQEAGIEIDPFMCAVLIGLARLITTCPMGYILEWWGRRRAGIISTLGMSVCMFLLAGHSQIEILKEVPYLPVVAIVGFIVLSTLGLYTLPFFMISELFPQKVRGPASGLTVAVGMFISFVVLKTYPGIKEYLGMSNCFIIFGVMALFALIFVYLALPETRRRTLLEIEEQFRSGRSKSQNQADVEMKEVFVRKPEE

Target ORF information:

RefSeq Version	NM_001259421.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8249), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259421.2

ORF Insert Sequence:

ATGGATGAGC AGCATCAGAA CCTCAATCAA AATGGCAGCG AATCCAATGG TAAGCAGGCC 
AGGATCGTTT CGGGACTTGA TCGTCTGCTG AAGACTCCTC CGCCTGGCGA ACAGACAAGA 
GCAGTGCGGC GACAAGTGAT CGCTGTTATC CTGGCCAACG TGGGTGTCTT CTCAACGGGA 
ATGACCCTGG CCATGCCCAC CGCCACTCTG CATCAGCTGA AGGACACCAC GGAGCCAGTG 
CATCTGAACG ATTCGCAGGC TTCCTGGTTC GCATCGGTAA ACGCTCTGTC CGCTCCTATA 
GGCGGCTTGC TGTCCGGTTT CTTGCTTGAC AGGATTGGAA GGAAGAAATC CCTGATAGTG 
CTCAATGTAC TGATTATTCT TGCTTGGATT CTGCTGGCCA CACCCAGTGA GAGCGACCAA 
AATGCCTTCT TTTGGCAACT AATTGTCTCA CGATTTATGC TGGGTGTTGG TATGGGACTG 
GCCAGTGCGC CACCAGGAGT CTATGCAGCC GAGATCAGTG TGCCCAAGAC GAGGGGCAGT 
CTTATACTGG GCACATCCAT TTCAGTGGCC GGTGGCATAA CGATTCTCTA CGGAATTGGT 
TACTGCATCC GCGATGATTT CCGACTGATT GCCCTCATCT GCTGTGGCTA CCAGCTGGTG 
GCCCTGCTCT GCGTGCTGCC ACTTCCGGAA AGTCATTGCT GGCTTCTGTC CAAGAAGCGT 
GTGACGGAGG CGAAGAGATC ACTTAACTAC TTCCGTGGAT TCAACAAATC GGATGAAATC 
ACCCATCCCC AAGTGCTCGA AGAGTTCCAG CTTCTGCAGA AATCCCTGCA GCAAAGGAAC 
ACCGCGGTGA AGGAGTCCTT TTGGCGTAAT CTGCACGAAC CGGAGGTCTA CAAGCCATTG 
GTCATCCTGA TGAGCTTGTT CGCCTTCCAG CAGCTAACCG GAATCTTTGT GGTCATCGTC 
TTCGCCGTGC AAATTTCCCA AGAGGCGGGC ATCGAAATCG ATCCCTTCAT GTGCGCCGTG 
CTCATTGGTT TGGCTCGCTT GATCACCACA TGCCCCATGG GATACATCCT GGAGTGGTGG 
GGTCGCCGAA GGGCAGGGAT TATCTCCACT TTGGGCATGT CCGTGTGCAT GTTCCTTTTG 
GCGGGACATA GCCAAATCGA GATCCTTAAG GAAGTCCCAT ATCTCCCGGT GGTGGCCATT 
GTGGGCTTCA TTGTGCTGAG CACTCTAGGT CTTTACACCC TGCCCTTCTT TATGATCTCC 
GAGCTTTTTC CGCAAAAGGT TCGAGGGCCC GCTTCAGGAC TCACCGTGGC CGTGGGCATG 
TTCATTTCGT TCGTGGTCTT GAAGACCTAT CCCGGCATCA AGGAATACCT GGGAATGTCG 
AATTGCTTCA TCATTTTTGG GGTCATGGCT CTGTTTGCCC TGATCTTCGT TTATCTCGCC 
CTCCCAGAGA CGCGTCGTCG AACGCTCTTG GAGATCGAGG AGCAGTTCCG ATCGGGTCGC 
AGTAAGTCTC AAAACCAGGC TGATGTTGAA ATGAAGGAGG TTTTTGTTCG AAAGCCCGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8249 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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