Khc-73 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Khc-73
Entrez Gene ID	36718
Full Name	Kinesin heavy chain 73
Synonyms	CG8183,DmKIN73,DmKin73,DmKlp73,Dmel\CG8183,KHC-73,KIF 13A,KIF13A/B,KLP73,Khc73,Kin73,Kinesin-73,anon-EST:fe3C9,khc-73
General protein information	Preferred Names Kinesin heavy chain 73 Names CG8183 gene product from transcript CG8183-RD CG8183-PA CG8183-PC CG8183-PD CG8183-PE CG8183-PF Khc-73-PA Khc-73-PC Khc-73-PD Khc-73-PE Khc-73-PF Kinesin-3 Kinesin-73 Kinesin3B anon-fast-evolving-3C9 kinesin heavy chain 73 kinesin heavy-chain 73
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52A6-52A10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299533.1	NP_001286462.1	kinesin heavy chain 73, isoform F
NM_001274071.2	NP_001261000.1	kinesin heavy chain 73, isoform E
NM_135357.5	NP_609201.3	kinesin heavy chain 73, isoform A
NM_176176.3	NP_788356.2	kinesin heavy chain 73, isoform C
NM_001274070.2	NP_001260999.1	kinesin heavy chain 73, isoform D

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KIF13B	XP_004935945.1
Drosophila melanogaster (fruit fly)	Khc-73	NP_001261000.1
Xenopus tropicalis (tropical clawed frog)	kif13b	XP_002939008.2
Canis lupus familiaris (dog)	KIF13B	XP_534562.2
Pan troglodytes (chimpanzee)	KIF13B	XP_001154346.3
Bos taurus (cattle)	KIF13B	XP_002689518.2
Homo sapiens (human)	KIF13B	NP_056069.2
	KIF13B	XP_002805344.1
Mus musculus (house mouse)	Kif13b	NP_001074646.1
Rattus norvegicus (Norway rat)	Kif13b	NP_998791.1
Danio rerio (zebrafish)	kif13ba	XP_005169925.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Khc73 stalk/14-3-3/NudE pathway defines a physical connection that coordinates the activities of multiple motor proteins to precisely position the spindle.
Title: A NudE/14-3-3 pathway coordinates dynein and the kinesin Khc73 to position the mitotic spindle.

the N-terminal half of Khc-73 can undergo a monomer-dimer transition to produce a fast processive motor and that its C-terminal half possesses a specific Rab5-vesicle binding domain
Title: Kinesin-73 is a processive motor that localizes to Rab5-containing organelles.

The following Khc-73 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Khc-73 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10096	NM_176176.3 Latest version!	Drosophila melanogaster kinesin heavy chain 73, transcript variant C (Khc-73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa10097	NM_135357.5 Latest version!	Drosophila melanogaster kinesin heavy chain 73, transcript variant A (Khc-73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12066	NM_001274070.2 Latest version!	Drosophila melanogaster kinesin heavy chain 73, transcript variant D (Khc-73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12067	NM_001274071.2 Latest version!	Drosophila melanogaster kinesin heavy chain 73, transcript variant E (Khc-73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa21824	NM_001299533.1 Latest version!	Drosophila melanogaster kinesin heavy chain 73, transcript variant F (Khc-73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Khc-73 ( NM_176176.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788356.2 Drosophila melanogaster kinesin heavy chain 73, transcript variant C (Khc-73), mRNA.

Price & Availability↑

CloneID	OFa10096
Clone ID Related Accession (Same CDS sequence)	NM_176176.3
Accession Version	NM_176176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin heavy chain 73, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176176.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGG ACGAGGATAA TGCCAAGCCA CAGGATCCGT TTTACGAGTC GCAAGAGAAC 
CACAATCTTA TTGGCGTGGC CAATATATTC TTGGAGGTTC TGTTTCATGA CGTCAAGCTG 
GACTACCACA CGCCCATCAT TAGCCAGCAA GGCGAGGTAG CGGGTCGGCT TCAGGTTGAG 
ATCGAGCGGA TTGCCGGCCA GATGCCACAG GACCGCATGT GCGAGTCAGT TTCAGAATCC 
TCTGGCGATT CACGGGATGA GTACGACGAC CCTGTGGATC CCACATCGAA TCAGATTACC 
TGCCGTGTGA CGATCAAGTG CGCCAGTGGA CTGCCATTGT CGCTCTCCAA CTTTGTCTTT 
TGCCAGTACA CTTTTTGGGG TCACCAAGAG ATGGTTGTTC CGGTTATCAA TGCTGAATCA 
ACGGCGCACG ACCAGAACAT GGTGTTTAAG TTTGAACACA CCCAGGACTT CACGGTTACC 
ATAAACGAAG AGTTTTTGGA GCACTGCATA GAAGGAGCTC TGTCCATTGA GGTGTGGGGC 
CATCGCAGTG CCGGCTTCTC CAAGACAAAG GGCTGGGAAG TCGAACAGCA GCAAGCCAAG 
GCCCGTTCCC TGGTCGATCG CTGGGCGGAG TTGTCGCGGA AGATCGAGCT TTGGGTGGAG 
ATTCACGAGC TTAATGACAA CGGCGAGTAT TCGCCAGTGG AGGTTACCAA TCGCAACGAG 
GTCTTGACCG GTGGCATTTA CCAGTTACGT CAGGGTCAGC AGCGGCGGGT GAATGTACGG 
GTGAAGCCGG TGCAAAACTC TGGCACCTTG CCCATTATCT GCCAGTCGAT TGTGAACGTT 
GCCATTGGCA GTGTGACAGT GCGATCTCGG TTGCAGCGAC CACTGGATTC TTACCAGGAG 
GAAGATCTCA CCGTGCTGCG CGAGAAGTGG AGCGAAGCAC TGGGACGAAG GCGTCAATAC 
CTTGACCAAC AGATCCAGAT GCTTATAAAG AAAGAGGAGA AGAACGAACA GGAGCGCGAG 
AGGGAGCTAA GCTTGGTTCA TCAGTGGGTC TCGTTGACGG AGGAACGCAA CGCGGTGTTG 
GTGCCAGCTC CTGGCTCGGG CATTCCCGGA GCACCTGCCT CATGGGAGCC GCCGTCCGGA 
ATGGAGCCAC ATGTGCCCGT CCTCTTCCTC AACCTGAACG GTGACGATCT GTCGGCGCAA 
AACACCAACG ACGAGCTCTC CGTCGCCGGC ATCAATTCCA TTCTCTCCAA GGAGCACGGA 
CATAAGTTTT ACACGCTGCA AATTCTGCAG CACCTAGATA AGGATGTTTG CTGTGTGGCC 
AGCTGGGACT CTTCGATGCA TGACAGTCAG GCCCTGAACC GTGTCACTGA GGCCAATGAG 
CGGGTCTATC TCATTCTGCG CACAACGGTA CGCCTCTCGC ACCCAGCACC TATGGATCTC 
GTGCTCCGCA AGCGACTAAG CATTAACATC AAGAAGGGAC AGACGCTAAC CGACCGCCTG 
AAGAAATTCC GTCTTGTGCG GGGAGAGAAC GCCATCTGGC AGAGCGGTGT CACCTATGAG 
GTGGTCTCTA ACATTCCAAA AGCCTCCGAG GAGTTGGAGG ACCGCGAGTC TCTTGCACAG 
TTGGCTGCCA GCGGAGATGA TTGCTCCGCA AGTGACGGCG AAACCTACAT AGAGAAATAC 
ACGCGCGGTG TTTCGGCGGT GGAGAGCATA CTGACTCTGG ATAGACTGCG GCAGAATGTG 
GCGGTCAAGG AGCTGGAAAC GGCACATGGA CAGCCGCTCA GCATGCGCAA GACCGTCAGC 
GTGCCGAACT TCTCACAGAT TATGCGCTTC GATGCATCGA TGGAGTCGCT GCTGAATGTG 
GGACGATCCG AGTCCTTTGC CGATCTCAAT AACAGCGCTT TGGGCAACAA GTTTACTCCA 
GCAGGTCATA GTCCAGCAGG AGCAGGCGGA GTCATCCGAA GTCGCCACAG CTTTGGAGGC 
AAAGGAAGCA GCGATGATTC TCCCGGAAAA GCCTTTGGCA TTGCTTCGCC AGCTACTAGT 
AAGCTGCTGG GCATGCGCAT GACTACGTTG CACGAGGAGC CACTGGGTGG ACACAGATCT 
CTCGACGAAG AGCCTGAGGA CAGCTACAGC GACTCGGAAT ATGCTGCCGA GTACGAACAG 
GAGCGGCAGC AGAACAAAAG CATGGCCACG CGATCCCGCC TCACGGCTTC CAAGACCATG 
GACTCATTCA TGGACGTCAG CAGCCATTCG AACCAAAGCT ACTTGAGCTA CACGTCCAGT 
GCCAACGCTA ACATGAAGCA CCTGACCGGC CTGGCGACTT TGAGCATGAG CTCATCCACC 
AGCAGTGGCT ACGGTTCCCA GGCTGTCTCC TGCAATAATC TGAGCAACGA GGATATTGCT 
TCAATGCGTT CCATGAGCAT TGATGAGACA CCAGATTTCG ATCGCGTTAA CTCGAATTCG 
CCACCAAACC GGCAGGCACG AGTTAACCCC TTTCTCAAGG ACATGCCCAA AGCTAAAATA 
CAAGAGCAAC CGGAACAGCA GGCCAAGAAG CTGCAGGAAG CATTTACGCA TCCGTTGGAG 
CAGCTGGAGT CCAGTCAAAA CGCACAAAGC GACGATGATG AATGCGCGCA ACTGCCAAAG 
AATAATAACA ACAACTTGGA CTTGGTAAAC GAGCCGAAGC CACTTTCAAG CCAAACGGAT 
CTGGAGGAAT CTATGTCGCA ACCTAAATCA AAAACGGAGT TTGCCACAGA CAATCAGAAC 
GGCAACAGGT CCTCTGACGA GTTAAGCCAC AGTTCCGAGG ATCTGCTCGA AGGCGATGGC 
ATCGTCCGGG AAGAGTTGCC CGCTGGAAAG GTGGTGCGGC GAAAGAAGTC CAATACCCAG 
CCCCCGAGCA ATGGCAACAG CATAAATAAC AATAACAACG GCACAACCCA GGCACCGCGC 
ATCAATCATC GGGCATCGGT GGCCAAAATG GAGGGTCTGG CTGCATATTT GGATTCTAGC 
ATTATGACCA GCAGCACCGA AGTTGATGAG GAGAGCAAGG ATGTGGAATT GGTTCTGCCC 
GAGTGGATAG TGGTGGGCGA GTCAGTGTTA ATCCGGCCGT ATAACACCAG CGGCGTCATT 
CGCTTTGTGG GAACCACGGA ATTCCAACCC GGTGCCTGGA TTGGCGTTGA ATTGGACACA 
CCAACGGGCA AAAACGACGG CAGCGTGAAG GGCGTTCAGT ATTTCCAGTG CAAGCCCAAG 
CACGGCATGT TTGTGCGCTC CGACAAGCTG ATGCTGGACA AGCGTGGCAA GGCGATGCGA 
GCCTACAAGG CCGCCGAGAA GAGCAACAGC ATCAGCAAAG AGATGAGTAC CTCGATGACT 
GGCTCGATGA CACGCTCCAA GAGCCGCGGC GATTCGCTAA ACCTTTCGGC GCGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788356.2
CDS	345..6044
Translation	MASDKIKVAVRVRPFNRREIELDTKCIVEMEKQQTILQNPPPLEKIERKQPKTFAFDHCFYSLNPEDENFASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGTQESKGIIPRLCDQLFSAIANKSTPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVDGLSQLAVTSYQDIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRMSLVDLAGSERAVKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTWLLKDNLGGNSRTVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVETLRSMLKHATGSPVGDVQDKLAESENLMKQISQTWEEKLVKTERIQNERQQALEKMGISVQASGIKVEKNKYYLVNLNADPSLNELLVYYLKDRTLIGGRTISGQQPDIQLSGLGIQPEHCVITIEDSGLYMEPVQGARCFVNGSAAVEKTPLQNGDRILWGNHHFFRVNSPKSNNTSMCASEPQTPAQLIDYNFARDEIMQNELSNDPIQTAIARLERQHEEDKQVALEKQRQEYERQFQQLRNILSPSTPYAPYAPYDPLRMGKITPNTPTSQMRVEKWAQERDEMFRRSLGQLKTDIMRANSLVQEANFLAEEMEKKTKFSVTLQIPPANLSPNRRRGAFVSEPAILVKRTNSGSQIWTMEKLENKLIDMREMYQEHKERVLNGLDEDNAKPQDPFYESQENHNLIGVANIFLEVLFHDVKLDYHTPIISQQGEVAGRLQVEIERIAGQMPQDRMCESVSESSGDSRDEYDDPVDPTSNQITCRVTIKCASGLPLSLSNFVFCQYTFWGHQEMVVPVINAESTAHDQNMVFKFEHTQDFTVTINEEFLEHCIEGALSIEVWGHRSAGFSKTKGWEVEQQQAKARSLVDRWAELSRKIELWVEIHELNDNGEYSPVEVTNRNEVLTGGIYQLRQGQQRRVNVRVKPVQNSGTLPIICQSIVNVAIGSVTVRSRLQRPLDSYQEEDLTVLREKWSEALGRRRQYLDQQIQMLIKKEEKNEQERERELSLVHQWVSLTEERNAVLVPAPGSGIPGAPASWEPPSGMEPHVPVLFLNLNGDDLSAQNTNDELSVAGINSILSKEHGHKFYTLQILQHLDKDVCCVASWDSSMHDSQALNRVTEANERVYLILRTTVRLSHPAPMDLVLRKRLSINIKKGQTLTDRLKKFRLVRGENAIWQSGVTYEVVSNIPKASEELEDRESLAQLAASGDDCSASDGETYIEKYTRGVSAVESILTLDRLRQNVAVKELETAHGQPLSMRKTVSVPNFSQIMRFDASMESLLNVGRSESFADLNNSALGNKFTPAGHSPAGAGGVIRSRHSFGGKGSSDDSPGKAFGIASPATSKLLGMRMTTLHEEPLGGHRSLDEEPEDSYSDSEYAAEYEQERQQNKSMATRSRLTASKTMDSFMDVSSHSNQSYLSYTSSANANMKHLTGLATLSMSSSTSSGYGSQAVSCNNLSNEDIASMRSMSIDETPDFDRVNSNSPPNRQARVNPFLKDMPKAKIQEQPEQQAKKLQEAFTHPLEQLESSQNAQSDDDECAQLPKNNNNNLDLVNEPKPLSSQTDLEESMSQPKSKTEFATDNQNGNRSSDELSHSSEDLLEGDGIVREELPAGKVVRRKKSNTQPPSNGNSINNNNNGTTQAPRINHRASVAKMEGLAAYLDSSIMTSSTEVDEESKDVELVLPEWIVVGESVLIRPYNTSGVIRFVGTTEFQPGAWIGVELDTPTGKNDGSVKGVQYFQCKPKHGMFVRSDKLMLDKRGKAMRAYKAAEKSNSISKEMSTSMTGSMTRSKSRGDSLNLSARK

Target ORF information:

RefSeq Version	NM_176176.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin heavy chain 73, transcript variant C (Khc-73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176176.3

ORF Insert Sequence:

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGG ACGAGGATAA TGCCAAGCCA CAGGATCCGT TTTACGAGTC GCAAGAGAAC 
CACAATCTTA TTGGCGTGGC CAATATATTC TTGGAGGTTC TGTTTCATGA CGTCAAGCTG 
GACTACCACA CGCCCATCAT TAGCCAGCAA GGCGAGGTAG CGGGTCGGCT TCAGGTTGAG 
ATCGAGCGGA TTGCCGGCCA GATGCCACAG GACCGCATGT GCGAGTCAGT TTCAGAATCC 
TCTGGCGATT CACGGGATGA GTACGACGAC CCTGTGGATC CCACATCGAA TCAGATTACC 
TGCCGTGTGA CGATCAAGTG CGCCAGTGGA CTGCCATTGT CGCTCTCCAA CTTTGTCTTT 
TGCCAGTACA CTTTTTGGGG TCACCAAGAG ATGGTTGTTC CGGTTATCAA TGCTGAATCA 
ACGGCGCACG ACCAGAACAT GGTGTTTAAG TTTGAACACA CCCAGGACTT CACGGTTACC 
ATAAACGAAG AGTTTTTGGA GCACTGCATA GAAGGAGCTC TGTCCATTGA GGTGTGGGGC 
CATCGCAGTG CCGGCTTCTC CAAGACAAAG GGCTGGGAAG TCGAACAGCA GCAAGCCAAG 
GCCCGTTCCC TGGTCGATCG CTGGGCGGAG TTGTCGCGGA AGATCGAGCT TTGGGTGGAG 
ATTCACGAGC TTAATGACAA CGGCGAGTAT TCGCCAGTGG AGGTTACCAA TCGCAACGAG 
GTCTTGACCG GTGGCATTTA CCAGTTACGT CAGGGTCAGC AGCGGCGGGT GAATGTACGG 
GTGAAGCCGG TGCAAAACTC TGGCACCTTG CCCATTATCT GCCAGTCGAT TGTGAACGTT 
GCCATTGGCA GTGTGACAGT GCGATCTCGG TTGCAGCGAC CACTGGATTC TTACCAGGAG 
GAAGATCTCA CCGTGCTGCG CGAGAAGTGG AGCGAAGCAC TGGGACGAAG GCGTCAATAC 
CTTGACCAAC AGATCCAGAT GCTTATAAAG AAAGAGGAGA AGAACGAACA GGAGCGCGAG 
AGGGAGCTAA GCTTGGTTCA TCAGTGGGTC TCGTTGACGG AGGAACGCAA CGCGGTGTTG 
GTGCCAGCTC CTGGCTCGGG CATTCCCGGA GCACCTGCCT CATGGGAGCC GCCGTCCGGA 
ATGGAGCCAC ATGTGCCCGT CCTCTTCCTC AACCTGAACG GTGACGATCT GTCGGCGCAA 
AACACCAACG ACGAGCTCTC CGTCGCCGGC ATCAATTCCA TTCTCTCCAA GGAGCACGGA 
CATAAGTTTT ACACGCTGCA AATTCTGCAG CACCTAGATA AGGATGTTTG CTGTGTGGCC 
AGCTGGGACT CTTCGATGCA TGACAGTCAG GCCCTGAACC GTGTCACTGA GGCCAATGAG 
CGGGTCTATC TCATTCTGCG CACAACGGTA CGCCTCTCGC ACCCAGCACC TATGGATCTC 
GTGCTCCGCA AGCGACTAAG CATTAACATC AAGAAGGGAC AGACGCTAAC CGACCGCCTG 
AAGAAATTCC GTCTTGTGCG GGGAGAGAAC GCCATCTGGC AGAGCGGTGT CACCTATGAG 
GTGGTCTCTA ACATTCCAAA AGCCTCCGAG GAGTTGGAGG ACCGCGAGTC TCTTGCACAG 
TTGGCTGCCA GCGGAGATGA TTGCTCCGCA AGTGACGGCG AAACCTACAT AGAGAAATAC 
ACGCGCGGTG TTTCGGCGGT GGAGAGCATA CTGACTCTGG ATAGACTGCG GCAGAATGTG 
GCGGTCAAGG AGCTGGAAAC GGCACATGGA CAGCCGCTCA GCATGCGCAA GACCGTCAGC 
GTGCCGAACT TCTCACAGAT TATGCGCTTC GATGCATCGA TGGAGTCGCT GCTGAATGTG 
GGACGATCCG AGTCCTTTGC CGATCTCAAT AACAGCGCTT TGGGCAACAA GTTTACTCCA 
GCAGGTCATA GTCCAGCAGG AGCAGGCGGA GTCATCCGAA GTCGCCACAG CTTTGGAGGC 
AAAGGAAGCA GCGATGATTC TCCCGGAAAA GCCTTTGGCA TTGCTTCGCC AGCTACTAGT 
AAGCTGCTGG GCATGCGCAT GACTACGTTG CACGAGGAGC CACTGGGTGG ACACAGATCT 
CTCGACGAAG AGCCTGAGGA CAGCTACAGC GACTCGGAAT ATGCTGCCGA GTACGAACAG 
GAGCGGCAGC AGAACAAAAG CATGGCCACG CGATCCCGCC TCACGGCTTC CAAGACCATG 
GACTCATTCA TGGACGTCAG CAGCCATTCG AACCAAAGCT ACTTGAGCTA CACGTCCAGT 
GCCAACGCTA ACATGAAGCA CCTGACCGGC CTGGCGACTT TGAGCATGAG CTCATCCACC 
AGCAGTGGCT ACGGTTCCCA GGCTGTCTCC TGCAATAATC TGAGCAACGA GGATATTGCT 
TCAATGCGTT CCATGAGCAT TGATGAGACA CCAGATTTCG ATCGCGTTAA CTCGAATTCG 
CCACCAAACC GGCAGGCACG AGTTAACCCC TTTCTCAAGG ACATGCCCAA AGCTAAAATA 
CAAGAGCAAC CGGAACAGCA GGCCAAGAAG CTGCAGGAAG CATTTACGCA TCCGTTGGAG 
CAGCTGGAGT CCAGTCAAAA CGCACAAAGC GACGATGATG AATGCGCGCA ACTGCCAAAG 
AATAATAACA ACAACTTGGA CTTGGTAAAC GAGCCGAAGC CACTTTCAAG CCAAACGGAT 
CTGGAGGAAT CTATGTCGCA ACCTAAATCA AAAACGGAGT TTGCCACAGA CAATCAGAAC 
GGCAACAGGT CCTCTGACGA GTTAAGCCAC AGTTCCGAGG ATCTGCTCGA AGGCGATGGC 
ATCGTCCGGG AAGAGTTGCC CGCTGGAAAG GTGGTGCGGC GAAAGAAGTC CAATACCCAG 
CCCCCGAGCA ATGGCAACAG CATAAATAAC AATAACAACG GCACAACCCA GGCACCGCGC 
ATCAATCATC GGGCATCGGT GGCCAAAATG GAGGGTCTGG CTGCATATTT GGATTCTAGC 
ATTATGACCA GCAGCACCGA AGTTGATGAG GAGAGCAAGG ATGTGGAATT GGTTCTGCCC 
GAGTGGATAG TGGTGGGCGA GTCAGTGTTA ATCCGGCCGT ATAACACCAG CGGCGTCATT 
CGCTTTGTGG GAACCACGGA ATTCCAACCC GGTGCCTGGA TTGGCGTTGA ATTGGACACA 
CCAACGGGCA AAAACGACGG CAGCGTGAAG GGCGTTCAGT ATTTCCAGTG CAAGCCCAAG 
CACGGCATGT TTGTGCGCTC CGACAAGCTG ATGCTGGACA AGCGTGGCAA GGCGATGCGA 
GCCTACAAGG CCGCCGAGAA GAGCAACAGC ATCAGCAAAG AGATGAGTAC CTCGATGACT 
GGCTCGATGA CACGCTCCAA GAGCCGCGGC GATTCGCTAA ACCTTTCGGC GCGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Khc-73 ( NM_135357.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609201.3 Drosophila melanogaster kinesin heavy chain 73, transcript variant A (Khc-73), mRNA.

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CloneID	OFa10097
Clone ID Related Accession (Same CDS sequence)	NM_135357.5
Accession Version	NM_135357.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin heavy chain 73, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_135357.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGC CCCTTATAGA GCCATTCTCA GAAGACGAGT TCGATGACAA GGACGAGGAT 
AATGCCAAGC CACAGGATCC GTTTTACGAG TCGCAAGAGA ACCACAATCT TATTGGCGTG 
GCCAATATAT TCTTGGAGGT TCTGTTTCAT GACGTCAAGC TGGACTACCA CACGCCCATC 
ATTAGCCAGC AAGGCGAGGT AGCGGGTCGG CTTCAGGTTG AGATCGAGCG GATTGCCGGC 
CAGATGCCAC AGGACCGCAT GTGCGAGTCA GTTTCAGAAT CCTCTGGCGA TTCACGGGAT 
GAGTACGACG ACCCTGTGGA TCCCACATCG AATCAGATTA CCTGCCGTGT GACGATCAAG 
TGCGCCAGTG GACTGCCATT GTCGCTCTCC AACTTTGTCT TTTGCCAGTA CACTTTTTGG 
GGTCACCAAG AGATGGTTGT TCCGGTTATC AATGCTGAAT CAACGGCGCA CGACCAGAAC 
ATGGTGTTTA AGTTTGAACA CACCCAGGAC TTCACGGTTA CCATAAACGA AGAGTTTTTG 
GAGCACTGCA TAGAAGGAGC TCTGTCCATT GAGGTGTGGG GCCATCGCAG TGCCGGCTTC 
TCCAAGACAA AGGGCTGGGA AGTCGAACAG CAGCAAGCCA AGGCCCGTTC CCTGGTCGAT 
CGCTGGGCGG AGTTGTCGCG GAAGATCGAG CTTTGGGTGG AGATTCACGA GCTTAATGAC 
AACGGCGAGT ATTCGCCAGT GGAGGTTACC AATCGCAACG AGGTCTTGAC CGGTGGCATT 
TACCAGTTAC GTCAGGGTCA GCAGCGGCGG GTGAATGTAC GGGTGAAGCC GGTGCAAAAC 
TCTGGCACCT TGCCCATTAT CTGCCAGTCG ATTGTGAACG TTGCCATTGG CAGTGTGACA 
GTGCGATCTC GGTTGCAGCG ACCACTGGAT TCTTACCAGG AGGAAGATCT CACCGTGCTG 
CGCGAGAAGT GGAGCGAAGC ACTGGGACGA AGGCGTCAAT ACCTTGACCA ACAGATCCAG 
ATGCTTATAA AGAAAGAGGA GAAGAACGAA CAGGAGCGCG AGAGGGAGCT AAGCTTGGTT 
CATCAGTGGG TCTCGTTGAC GGAGGAACGC AACGCGGTGT TGGTGCCAGC TCCTGGCTCG 
GGCATTCCCG GAGCACCTGC CTCATGGGAG CCGCCGTCCG GAATGGAGCC ACATGTGCCC 
GTCCTCTTCC TCAACCTGAA CGGTGACGAT CTGTCGGCGC AAAACACCAA CGACGAGCTC 
TCCGTCGCCG GCATCAATTC CATTCTCTCC AAGGAGCACG GACATAAGTT TTACACGCTG 
CAAATTCTGC AGCACCTAGA TAAGGATGTT TGCTGTGTGG CCAGCTGGGA CTCTTCGATG 
CATGACAGTC AGGCCCTGAA CCGTGTCACT GAGGCCAATG AGCGGGTCTA TCTCATTCTG 
CGCACAACGG TACGCCTCTC GCACCCAGCA CCTATGGATC TCGTGCTCCG CAAGCGACTA 
AGCATTAACA TCAAGAAGGG ACAGACGCTA ACCGACCGCC TGAAGAAATT CCGTCTTGTG 
CGGGGAGAGA ACGCCATCTG GCAGAGCGGT GTCACCTATG AGGTGGTCTC TAACATTCCA 
AAAGCCTCCG AGGAGTTGGA GGACCGCGAG TCTCTTGCAC AGTTGGCTGC CAGCGGAGAT 
GATTGCTCCG CAAGTGACGG CGAAACCTAC ATAGAGAAAT ACACGCGCGG TGTTTCGGCG 
GTGGAGAGCA TACTGACTCT GGATAGACTG CGGCAGAATG TGGCGGTCAA GGAGCTGGAA 
ACGGCACATG GACAGCCGCT CAGCATGCGC AAGACCGTCA GCGTGCCGAA CTTCTCACAG 
CAACTCATAA ATAAACTTAC GCAGATTATG CGCTTCGATG CATCGATGGA GTCGCTGCTG 
AATGTGGGAC GATCCGAGTC CTTTGCCGAT CTCAATAACA GCGCTTTGGG CAACAAGTTT 
ACTCCAGCAG GTCATAGTCC AGCAGGAGCA GGCGGAGTCA TCCGAAGTCG CCACAGCTTT 
GGAGGCAAAG GAAGCAGCGA TGATTCTCCC GGAAAAGCCT TTGGCATTGC TTCGCCAGCT 
ACTAGTAAGC TGCTGGGCAT GCGCATGACT ACGTTGCACG AGGAGCCACT GGGTGGACAC 
AGATCTCTCG ACGAAGAGCC TGAGGACAGC TACAGCGACT CGGAATATGC TGCCGAGTAC 
GAACAGGAGC GGCAGCAGAA CAAAAGCATG GCCACGCGAT CCCGCCTCAC GGCTTCCAAG 
ACCATGGACT CATTCATGGA CGTCAGCAGC CATTCGAACC AAAGCTACTT GAGCTACACG 
TCCAGTGCCA ACGCTAACAT GAAGCACCTG ACCGGCCTGG CGACTTTGAG CATGAGCTCA 
TCCACCAGCA GTGGCTACGG TTCCCAGGCT GTCTCCTGCA ATAATCTGAG CAACGAGGAT 
ATTGCTTCAA TGCGTTCCAT GAGCATTGAT GAGACACCAG ATTTCGATCG CGTTAACTCG 
AATTCGCCAC CAAACCGGCA GGCACGAGTT AACCCCTTTC TCAAGGACAT GCCCAAAGCT 
AAAATACAAG AGCAACCGGA ACAGCAGGCC AAGAAGCTGC AGGAAGCATT TACGCATCCG 
TTGGAGCAGC TGGAGTCCAG TCAAAACGCA CAAAGCGACG ATGATGAATG CGCGCAACTG 
CCAAAGAATA ATAACAACAA CTTGGACTTG GTAAACGAGC CGAAGCCACT TTCAAGCCAA 
ACGGATCTGG AGGAATCTAT GTCGCAACCT AAATCAAAAA CGGAGTTTGC CACAGACAAT 
CAGAACGGCA ACAGGTCCTC TGACGAGTTA AGCCACAGTT CCGAGGATCT GCTCGAAGGC 
GATGGCATCG TCCGGGAAGA GTTGCCCGCT GGAAAGGTGG TGCGGCGAAA GAAGTCCAAT 
ACCCAGCCCC CGAGCAATGG CAACAGCATA AATAACAATA ACAACGGCAC AACCCAGGCA 
CCGCGCATCA ATCATCGGGC ATCGGTGGCC AAAATGGAGG GTCTGGCTGC ATATTTGGAT 
TCTAGCATTA TGACCAGCAG CACCGAAGTT GATGAGGAGA GCAAGGATGT GGAATTGGTT 
CTGCCCGAGT GGATAGTGGT GGGCGAGTCA GTGTTAATCC GGCCGTATAA CACCAGCGGC 
GTCATTCGCT TTGTGGGAAC CACGGAATTC CAACCCGGTG CCTGGATTGG CGTTGAATTG 
GACACACCAA CGGGCAAAAA CGACGGCAGC GTGAAGGGCG TTCAGTATTT CCAGTGCAAG 
CCCAAGCACG GCATGTTTGT GCGCTCCGAC AAGCTGATGC TGGACAAGCG TGGCAAGGCG 
ATGCGAGCCT ACAAGGCCGC CGAGAAGAGC AACAGCATCA GCAAAGAGAT GAGTACCTCG 
ATGACTGGCT CGATGACACG CTCCAAGAGC CGCGGCGATT CGCTAAACCT TTCGGCGCGT 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609201.3
CDS	345..6110
Translation	MASDKIKVAVRVRPFNRREIELDTKCIVEMEKQQTILQNPPPLEKIERKQPKTFAFDHCFYSLNPEDENFASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGTQESKGIIPRLCDQLFSAIANKSTPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVDGLSQLAVTSYQDIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRMSLVDLAGSERAVKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTWLLKDNLGGNSRTVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVETLRSMLKHATGSPVGDVQDKLAESENLMKQISQTWEEKLVKTERIQNERQQALEKMGISVQASGIKVEKNKYYLVNLNADPSLNELLVYYLKDRTLIGGRTISGQQPDIQLSGLGIQPEHCVITIEDSGLYMEPVQGARCFVNGSAAVEKTPLQNGDRILWGNHHFFRVNSPKSNNTSMCASEPQTPAQLIDYNFARDEIMQNELSNDPIQTAIARLERQHEEDKQVALEKQRQEYERQFQQLRNILSPSTPYAPYAPYDPLRMGKITPNTPTSQMRVEKWAQERDEMFRRSLGQLKTDIMRANSLVQEANFLAEEMEKKTKFSVTLQIPPANLSPNRRRGAFVSEPAILVKRTNSGSQIWTMEKLENKLIDMREMYQEHKERVLNGLPLIEPFSEDEFDDKDEDNAKPQDPFYESQENHNLIGVANIFLEVLFHDVKLDYHTPIISQQGEVAGRLQVEIERIAGQMPQDRMCESVSESSGDSRDEYDDPVDPTSNQITCRVTIKCASGLPLSLSNFVFCQYTFWGHQEMVVPVINAESTAHDQNMVFKFEHTQDFTVTINEEFLEHCIEGALSIEVWGHRSAGFSKTKGWEVEQQQAKARSLVDRWAELSRKIELWVEIHELNDNGEYSPVEVTNRNEVLTGGIYQLRQGQQRRVNVRVKPVQNSGTLPIICQSIVNVAIGSVTVRSRLQRPLDSYQEEDLTVLREKWSEALGRRRQYLDQQIQMLIKKEEKNEQERERELSLVHQWVSLTEERNAVLVPAPGSGIPGAPASWEPPSGMEPHVPVLFLNLNGDDLSAQNTNDELSVAGINSILSKEHGHKFYTLQILQHLDKDVCCVASWDSSMHDSQALNRVTEANERVYLILRTTVRLSHPAPMDLVLRKRLSINIKKGQTLTDRLKKFRLVRGENAIWQSGVTYEVVSNIPKASEELEDRESLAQLAASGDDCSASDGETYIEKYTRGVSAVESILTLDRLRQNVAVKELETAHGQPLSMRKTVSVPNFSQQLINKLTQIMRFDASMESLLNVGRSESFADLNNSALGNKFTPAGHSPAGAGGVIRSRHSFGGKGSSDDSPGKAFGIASPATSKLLGMRMTTLHEEPLGGHRSLDEEPEDSYSDSEYAAEYEQERQQNKSMATRSRLTASKTMDSFMDVSSHSNQSYLSYTSSANANMKHLTGLATLSMSSSTSSGYGSQAVSCNNLSNEDIASMRSMSIDETPDFDRVNSNSPPNRQARVNPFLKDMPKAKIQEQPEQQAKKLQEAFTHPLEQLESSQNAQSDDDECAQLPKNNNNNLDLVNEPKPLSSQTDLEESMSQPKSKTEFATDNQNGNRSSDELSHSSEDLLEGDGIVREELPAGKVVRRKKSNTQPPSNGNSINNNNNGTTQAPRINHRASVAKMEGLAAYLDSSIMTSSTEVDEESKDVELVLPEWIVVGESVLIRPYNTSGVIRFVGTTEFQPGAWIGVELDTPTGKNDGSVKGVQYFQCKPKHGMFVRSDKLMLDKRGKAMRAYKAAEKSNSISKEMSTSMTGSMTRSKSRGDSLNLSARK

Target ORF information:

RefSeq Version	NM_135357.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin heavy chain 73, transcript variant A (Khc-73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135357.5

ORF Insert Sequence:

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGC CCCTTATAGA GCCATTCTCA GAAGACGAGT TCGATGACAA GGACGAGGAT 
AATGCCAAGC CACAGGATCC GTTTTACGAG TCGCAAGAGA ACCACAATCT TATTGGCGTG 
GCCAATATAT TCTTGGAGGT TCTGTTTCAT GACGTCAAGC TGGACTACCA CACGCCCATC 
ATTAGCCAGC AAGGCGAGGT AGCGGGTCGG CTTCAGGTTG AGATCGAGCG GATTGCCGGC 
CAGATGCCAC AGGACCGCAT GTGCGAGTCA GTTTCAGAAT CCTCTGGCGA TTCACGGGAT 
GAGTACGACG ACCCTGTGGA TCCCACATCG AATCAGATTA CCTGCCGTGT GACGATCAAG 
TGCGCCAGTG GACTGCCATT GTCGCTCTCC AACTTTGTCT TTTGCCAGTA CACTTTTTGG 
GGTCACCAAG AGATGGTTGT TCCGGTTATC AATGCTGAAT CAACGGCGCA CGACCAGAAC 
ATGGTGTTTA AGTTTGAACA CACCCAGGAC TTCACGGTTA CCATAAACGA AGAGTTTTTG 
GAGCACTGCA TAGAAGGAGC TCTGTCCATT GAGGTGTGGG GCCATCGCAG TGCCGGCTTC 
TCCAAGACAA AGGGCTGGGA AGTCGAACAG CAGCAAGCCA AGGCCCGTTC CCTGGTCGAT 
CGCTGGGCGG AGTTGTCGCG GAAGATCGAG CTTTGGGTGG AGATTCACGA GCTTAATGAC 
AACGGCGAGT ATTCGCCAGT GGAGGTTACC AATCGCAACG AGGTCTTGAC CGGTGGCATT 
TACCAGTTAC GTCAGGGTCA GCAGCGGCGG GTGAATGTAC GGGTGAAGCC GGTGCAAAAC 
TCTGGCACCT TGCCCATTAT CTGCCAGTCG ATTGTGAACG TTGCCATTGG CAGTGTGACA 
GTGCGATCTC GGTTGCAGCG ACCACTGGAT TCTTACCAGG AGGAAGATCT CACCGTGCTG 
CGCGAGAAGT GGAGCGAAGC ACTGGGACGA AGGCGTCAAT ACCTTGACCA ACAGATCCAG 
ATGCTTATAA AGAAAGAGGA GAAGAACGAA CAGGAGCGCG AGAGGGAGCT AAGCTTGGTT 
CATCAGTGGG TCTCGTTGAC GGAGGAACGC AACGCGGTGT TGGTGCCAGC TCCTGGCTCG 
GGCATTCCCG GAGCACCTGC CTCATGGGAG CCGCCGTCCG GAATGGAGCC ACATGTGCCC 
GTCCTCTTCC TCAACCTGAA CGGTGACGAT CTGTCGGCGC AAAACACCAA CGACGAGCTC 
TCCGTCGCCG GCATCAATTC CATTCTCTCC AAGGAGCACG GACATAAGTT TTACACGCTG 
CAAATTCTGC AGCACCTAGA TAAGGATGTT TGCTGTGTGG CCAGCTGGGA CTCTTCGATG 
CATGACAGTC AGGCCCTGAA CCGTGTCACT GAGGCCAATG AGCGGGTCTA TCTCATTCTG 
CGCACAACGG TACGCCTCTC GCACCCAGCA CCTATGGATC TCGTGCTCCG CAAGCGACTA 
AGCATTAACA TCAAGAAGGG ACAGACGCTA ACCGACCGCC TGAAGAAATT CCGTCTTGTG 
CGGGGAGAGA ACGCCATCTG GCAGAGCGGT GTCACCTATG AGGTGGTCTC TAACATTCCA 
AAAGCCTCCG AGGAGTTGGA GGACCGCGAG TCTCTTGCAC AGTTGGCTGC CAGCGGAGAT 
GATTGCTCCG CAAGTGACGG CGAAACCTAC ATAGAGAAAT ACACGCGCGG TGTTTCGGCG 
GTGGAGAGCA TACTGACTCT GGATAGACTG CGGCAGAATG TGGCGGTCAA GGAGCTGGAA 
ACGGCACATG GACAGCCGCT CAGCATGCGC AAGACCGTCA GCGTGCCGAA CTTCTCACAG 
CAACTCATAA ATAAACTTAC GCAGATTATG CGCTTCGATG CATCGATGGA GTCGCTGCTG 
AATGTGGGAC GATCCGAGTC CTTTGCCGAT CTCAATAACA GCGCTTTGGG CAACAAGTTT 
ACTCCAGCAG GTCATAGTCC AGCAGGAGCA GGCGGAGTCA TCCGAAGTCG CCACAGCTTT 
GGAGGCAAAG GAAGCAGCGA TGATTCTCCC GGAAAAGCCT TTGGCATTGC TTCGCCAGCT 
ACTAGTAAGC TGCTGGGCAT GCGCATGACT ACGTTGCACG AGGAGCCACT GGGTGGACAC 
AGATCTCTCG ACGAAGAGCC TGAGGACAGC TACAGCGACT CGGAATATGC TGCCGAGTAC 
GAACAGGAGC GGCAGCAGAA CAAAAGCATG GCCACGCGAT CCCGCCTCAC GGCTTCCAAG 
ACCATGGACT CATTCATGGA CGTCAGCAGC CATTCGAACC AAAGCTACTT GAGCTACACG 
TCCAGTGCCA ACGCTAACAT GAAGCACCTG ACCGGCCTGG CGACTTTGAG CATGAGCTCA 
TCCACCAGCA GTGGCTACGG TTCCCAGGCT GTCTCCTGCA ATAATCTGAG CAACGAGGAT 
ATTGCTTCAA TGCGTTCCAT GAGCATTGAT GAGACACCAG ATTTCGATCG CGTTAACTCG 
AATTCGCCAC CAAACCGGCA GGCACGAGTT AACCCCTTTC TCAAGGACAT GCCCAAAGCT 
AAAATACAAG AGCAACCGGA ACAGCAGGCC AAGAAGCTGC AGGAAGCATT TACGCATCCG 
TTGGAGCAGC TGGAGTCCAG TCAAAACGCA CAAAGCGACG ATGATGAATG CGCGCAACTG 
CCAAAGAATA ATAACAACAA CTTGGACTTG GTAAACGAGC CGAAGCCACT TTCAAGCCAA 
ACGGATCTGG AGGAATCTAT GTCGCAACCT AAATCAAAAA CGGAGTTTGC CACAGACAAT 
CAGAACGGCA ACAGGTCCTC TGACGAGTTA AGCCACAGTT CCGAGGATCT GCTCGAAGGC 
GATGGCATCG TCCGGGAAGA GTTGCCCGCT GGAAAGGTGG TGCGGCGAAA GAAGTCCAAT 
ACCCAGCCCC CGAGCAATGG CAACAGCATA AATAACAATA ACAACGGCAC AACCCAGGCA 
CCGCGCATCA ATCATCGGGC ATCGGTGGCC AAAATGGAGG GTCTGGCTGC ATATTTGGAT 
TCTAGCATTA TGACCAGCAG CACCGAAGTT GATGAGGAGA GCAAGGATGT GGAATTGGTT 
CTGCCCGAGT GGATAGTGGT GGGCGAGTCA GTGTTAATCC GGCCGTATAA CACCAGCGGC 
GTCATTCGCT TTGTGGGAAC CACGGAATTC CAACCCGGTG CCTGGATTGG CGTTGAATTG 
GACACACCAA CGGGCAAAAA CGACGGCAGC GTGAAGGGCG TTCAGTATTT CCAGTGCAAG 
CCCAAGCACG GCATGTTTGT GCGCTCCGAC AAGCTGATGC TGGACAAGCG TGGCAAGGCG 
ATGCGAGCCT ACAAGGCCGC CGAGAAGAGC AACAGCATCA GCAAAGAGAT GAGTACCTCG 
ATGACTGGCT CGATGACACG CTCCAAGAGC CGCGGCGATT CGCTAAACCT TTCGGCGCGT 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Khc-73 ( NM_001274070.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260999.1 Drosophila melanogaster kinesin heavy chain 73, transcript variant D (Khc-73), mRNA.

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CloneID	OFa12066
Clone ID Related Accession (Same CDS sequence)	NM_001274070.2
Accession Version	NM_001274070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin heavy chain 73, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274070.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAC AGCAGACGAT ACTGCAGAAT CCGCCGCCAC TGGAAAAAAT AGAGAGAAAA 
CAACCAAAGA CATTCGCATT CGATCACTGC TTTTACTCAT TGAACCCCGA GGACGAGAAC 
TTTGCGTCCC AGGAGACAGT GTTCGATTGC GTGGGACGTG GAATTCTGGA TAATGCATTC 
CAGGGCTATA ATGCGTGCAT ATTCGCTTAC GGCCAGACAG GTTCTGGCAA GTCCTACACG 
ATGATGGGCA CCCAGGAGAG CAAGGGCATC ATTCCACGTC TGTGTGACCA ACTCTTCTCG 
GCCATAGCCA ACAAATCCAC ACCCGAGCTT ATGTACAAGG TGGAGGTGTC CTACATGGAG 
ATCTATAACG AGAAGGTCCA CGATCTGCTC GATCCCAAGC CGAACAAACA GTCGCTTAAG 
GTGCGCGAGC ACAATGTTAT GGGCCCATAT GTGGACGGAC TGTCGCAGCT GGCTGTGACA 
TCCTATCAGG ACATCGATAA CCTCATGACC GAAGGCAACA AGTCACGAAC GGTGGCCGCC 
ACCAATATGA ATGCCGAGTC GTCGCGCTCC CACGCCGTCT TCTCAGTGGT CCTCACTCAG 
ATACTCACGG ATCAGGCGAC GGGAGTGAGC GGCGAGAAGG TGTCCCGCAT GTCCCTGGTG 
GATTTGGCTG GCTCCGAGCG GGCTGTGAAA ACGGGAGCTG TTGGCGATCG TCTCAAGGAA 
GGCTCTAACA TCAACAAATC TCTGACCACA CTTGGCCTGG TCATCTCCAA GTTGGCCGAT 
CAATCCAATG GCAAGAAGAG CGGTAACGAC AAATTCGTTC CCTACCGCGA TTCCGTGCTC 
ACCTGGCTGC TGAAAGACAA TTTGGGTGGC AATTCCAGAA CTGTTATGGT AGCGACAATC 
TCGCCATCGG CAGATAACTA CGAGGAGACG CTTTCCACGC TGCGTTATGC AGATCGAGCC 
AAGCGCATTG TTAACCACGC TGTTGTCAAC GAAGATCCCA ATGCCCGCAT CATTCGTGAG 
CTGCGACACG AGGTGGAGAC CCTTAGAAGT ATGCTGAAAC ACGCCACTGG TTCACCAGTG 
GGCGATGTCC AGGATAAGCT GGCTGAGAGC GAGAACCTGA TGAAACAGAT TTCGCAGACT 
TGGGAGGAGA AGCTGGTTAA GACAGAACGC ATTCAAAACG AACGGCAGCA GGCTCTAGAG 
AAAATGGGCA TTAGTGTACA GGCCAGTGGC ATCAAGGTAG AGAAGAACAA GTACTACTTG 
GTCAATTTAA ATGCTGATCC GTCCCTTAAC GAGCTGCTGG TCTATTATCT GAAGGATCGC 
ACACTGATTG GCGGACGCAC TATCAGTGGC CAGCAGCCGG ATATACAACT TTCCGGTCTC 
GGAATCCAGC CCGAGCACTG TGTGATCACT ATCGAGGATA GTGGACTGTA CATGGAGCCA 
GTGCAGGGAG CGCGTTGCTT TGTGAACGGA TCTGCTGCTG TGGAAAAGAC ACCACTCCAG 
AACGGCGACC GTATCCTGTG GGGCAACCAC CACTTTTTCC GCGTCAACTC GCCGAAGAGC 
AATAACACTA GTATGTGCGC TTCGGAGCCC CAGACGCCGG CGCAACTGAT TGATTACAAT 
TTCGCACGCG ATGAGATTAT GCAGAACGAA TTGAGCAACG ATCCTATCCA GACGGCCATT 
GCTCGGCTGG AACGTCAGCA CGAGGAAGAT AAGCAGGTGG CGCTTGAAAA ACAACGGCAG 
GAGTACGAGC GTCAGTTCCA GCAGCTACGC AATATTCTGT CGCCTAGTAC ACCATATGCA 
CCTTATGCTC CTTATGATCC ACTGCGCATG GGTAAGATTA CCCCAAATAC TCCTACTTCG 
CAAATGCGAG TGGAAAAGTG GGCACAGGAA CGAGATGAGA TGTTTCGTCG CAGTTTGGGT 
CAGCTGAAAA CGGATATTAT GCGTGCGAAT TCCCTGGTCC AGGAGGCCAA CTTTCTGGCT 
GAGGAGATGG AGAAGAAGAC CAAGTTTTCA GTCACTCTGC AGATTCCGCC AGCTAATCTA 
AGCCCTAACA GAAGGCGTGG GGCATTTGTC AGCGAACCCG CCATTCTGGT GAAGCGCACA 
AACTCTGGTA GCCAGATCTG GACGATGGAG AAGCTGGAAA ACAAGCTGAT AGACATGCGC 
GAGATGTACC AGGAGCACAA GGAGCGCGTT CTGAACGGAC TGGACGAGGA TAATGCCAAG 
CCACAGGATC CGTTTTACGA GTCGCAAGAG AACCACAATC TTATTGGCGT GGCCAATATA 
TTCTTGGAGG TTCTGTTTCA TGACGTCAAG CTGGACTACC ACACGCCCAT CATTAGCCAG 
CAAGGCGAGG TAGCGGGTCG GCTTCAGGTT GAGATCGAGC GGATTGCCGG CCAGATGCCA 
CAGGACCGCA TGTGCGAGTC AGTTTCAGAA TCCTCTGGCG ATTCACGGGA TGAGTACGAC 
GACCCTGTGG ATCCCACATC GAATCAGATT ACCTGCCGTG TGACGATCAA GTGCGCCAGT 
GGACTGCCAT TGTCGCTCTC CAACTTTGTC TTTTGCCAGT ACACTTTTTG GGGTCACCAA 
GAGATGGTTG TTCCGGTTAT CAATGCTGAA TCAACGGCGC ACGACCAGAA CATGGTGTTT 
AAGTTTGAAC ACACCCAGGA CTTCACGGTT ACCATAAACG AAGAGTTTTT GGAGCACTGC 
ATAGAAGGAG CTCTGTCCAT TGAGGTGTGG GGCCATCGCA GTGCCGGCTT CTCCAAGACA 
AAGGGCTGGG AAGTCGAACA GCAGCAAGCC AAGGCCCGTT CCCTGGTCGA TCGCTGGGCG 
GAGTTGTCGC GGAAGATCGA GCTTTGGGTG GAGATTCACG AGCTTAATGA CAACGGCGAG 
TATTCGCCAG TGGAGGTTAC CAATCGCAAC GAGGTCTTGA CCGGTGGCAT TTACCAGTTA 
CGTCAGGGTC AGCAGCGGCG GGTGAATGTA CGGGTGAAGC CGGTGCAAAA CTCTGGCACC 
TTGCCCATTA TCTGCCAGTC GATTGTGAAC GTTGCCATTG GCAGTGTGAC AGTGCGATCT 
CGGTTGCAGC GACCACTGGA TTCTTACCAG GAGGAAGATC TCACCGTGCT GCGCGAGAAG 
TGGAGCGAAG CACTGGGACG AAGGCGTCAA TACCTTGACC AACAGATCCA GATGCTTATA 
AAGAAAGAGG AGAAGAACGA ACAGGAGCGC GAGAGGGAGC TAAGCTTGGT TCATCAGTGG 
GTCTCGTTGA CGGAGGAACG CAACGCGGTG TTGGTGCCAG CTCCTGGCTC GGGCATTCCC 
GGAGCACCTG CCTCATGGGA GCCGCCGTCC GGAATGGAGC CACATGTGCC CGTCCTCTTC 
CTCAACCTGA ACGGTGACGA TCTGTCGGCG CAAAACACCA ACGACGAGCT CTCCGTCGCC 
GGCATCAATT CCATTCTCTC CAAGGAGCAC GGACATAAGT TTTACACGCT GCAAATTCTG 
CAGCACCTAG ATAAGGATGT TTGCTGTGTG GCCAGCTGGG ACTCTTCGAT GCATGACAGT 
CAGGCCCTGA ACCGTGTCAC TGAGGCCAAT GAGCGGGTCT ATCTCATTCT GCGCACAACG 
GTACGCCTCT CGCACCCAGC ACCTATGGAT CTCGTGCTCC GCAAGCGACT AAGCATTAAC 
ATCAAGAAGG GACAGACGCT AACCGACCGC CTGAAGAAAT TCCGTCTTGT GCGGGGAGAG 
AACGCCATCT GGCAGAGCGG TGTCACCTAT GAGGTGGTCT CTAACATTCC AAAAGCCTCC 
GAGGAGTTGG AGGACCGCGA GTCTCTTGCA CAGTTGGCTG CCAGCGGAGA TGATTGCTCC 
GCAAGTGACG GCGAAACCTA CATAGAGAAA TACACGCGCG GTGTTTCGGC GGTGGAGAGC 
ATACTGACTC TGGATAGACT GCGGCAGAAT GTGGCGGTCA AGGAGCTGGA AACGGCACAT 
GGACAGCCGC TCAGCATGCG CAAGACCGTC AGCGTGCCGA ACTTCTCACA GGCGGTCAAA 
GACACCACCA ATACCGGGAG TCAACTCATA AATAAACTTA CGCAGATTAT GCGCTTCGAT 
GCATCGATGG AGTCGCTGCT GAATGTGGGA CGATCCGAGT CCTTTGCCGA TCTCAATAAC 
AGCGCTTTGG GCAACAAGTT TACTCCAGGT CATAGTCCAG CAGGAGCAGG CGGAGTCATC 
CGAAGTCGCC ACAGCTTTGG AGGCAAAGGA AGCAGCGATG ATTCTCCCGG AAAAGCCTTT 
GGCATTGCGC GTCCAACATT TTTGAATCTC AATTTGAACT TGAACACTTT GAGAATTGCG 
CCAACGAAAC CTTCGCCAGC TACTAGTAAG CTGCTGGGCA TGCGCATGAC TACGTTGCAC 
GAGGAGCCAC TGGGTGGACA CAGATCTCTC GACGAAGAGC CTGAGGACAG CTACAGCGAC 
TCGGAATATG CTGCCGAGTA CGAACAGGAG CGGCAGCAGA ACAAAAGCAT GGCCACGCGA 
TCCCGCCTCA CGGCTTCCAA GACCATGGAC TCATTCATGG ACGTCAGCAG CCATTCGAAC 
CAAAGCTACT TGAGCTACAC GTCCAGTGCC AACGCTAACA TGAAGCACCT GACCGGCCTG 
GCGACTTTGA GCATGAGCTC ATCCACCAGC AGTGGCTACG GTTCCCAGGC TGTCTCCTGC 
AATAATCTGA GCAACGAGGA TATTGCTTCA ATGCGTTCCA TGAGCATTGA TGAGACACCA 
GATTTCGATC GCGTTAACTC GAATTCGCCA CCAAACCGGC AGGCACGAGT TAACCCCTTT 
CTCAAGGACA TGCCCAAAGC TAAAATACAA GAGCAACCGG AACAGCAGGC CAAGAAGCTG 
CAGGAAGCAT TTACGCATCC GTTGGAGCAG CTGGAGTCCA GTCAAAACGC ACAAAGCGAC 
GATGATGAAT GCGCGCAACT GCCAAAGAAT AATAACAACA ACTTGGACTT GGTAAACGAG 
CCGAAGCCAC TTTCAAGCCA AACGGATCTG GAGGAATCTA TGTCGCAACC TAAATCAAAA 
ACGGAGTTTG CCACAGACAA TCAGAACGGC AACAGGTCCT CTGACGAGTT AAGCCACAGT 
TCCGAGGATC TGCTCGAAGG CGATGGCATC GTCCGGGAAG AGTTGCCCGC TGGAAAGGTG 
GTGCGGCGAA AGAAGTCCAA TACCCAGCCC CCGAGCAATG GCAACAGCAT AAATAACAAT 
AACAACGGCA CAACCCAGGC ACCGCGCATC AATCATCGGG CATCGGTGGC CAAAATGGAG 
GGTCTGGCTG CATATTTGGA TTCTAGCATT ATGACCAGCA GCACCGAAGT TGATGAGGAG 
AGCAAGGATG TGGAATTGGT TCTGCCCGAG TGGATAGTGG TGGGCGAGTC AGTGTTAATC 
CGGCCGTATA ACACCAGCGG CGTCATTCGC TTTGTGGGAA CCACGGAATT CCAACCCGGT 
GCCTGGATTG GCGTTGAATT GGACACACCA ACGGGCAAAA ACGACGGCAG CGTGAAGGGC 
GTTCAGTATT TCCAGTGCAA GCCCAAGCAC GGCATGTTTG TGCGCTCCGA CAAGCTGATG 
CTGGACAAGC GTGGCAAGGC GATGCGAGCC TACAAGGCCG CCGAGAAGAG CAACAGCATC 
AGCAAAGAGA TGAGTACCTC GATGACTGGC TCGATGACAC GCTCCAAGAG CCGCGGCGAT 
TCGCTAAACC TTTCGGCGCG TAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260999.1
CDS	171..5897
Translation	MEKQQTILQNPPPLEKIERKQPKTFAFDHCFYSLNPEDENFASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGTQESKGIIPRLCDQLFSAIANKSTPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVDGLSQLAVTSYQDIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRMSLVDLAGSERAVKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTWLLKDNLGGNSRTVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVETLRSMLKHATGSPVGDVQDKLAESENLMKQISQTWEEKLVKTERIQNERQQALEKMGISVQASGIKVEKNKYYLVNLNADPSLNELLVYYLKDRTLIGGRTISGQQPDIQLSGLGIQPEHCVITIEDSGLYMEPVQGARCFVNGSAAVEKTPLQNGDRILWGNHHFFRVNSPKSNNTSMCASEPQTPAQLIDYNFARDEIMQNELSNDPIQTAIARLERQHEEDKQVALEKQRQEYERQFQQLRNILSPSTPYAPYAPYDPLRMGKITPNTPTSQMRVEKWAQERDEMFRRSLGQLKTDIMRANSLVQEANFLAEEMEKKTKFSVTLQIPPANLSPNRRRGAFVSEPAILVKRTNSGSQIWTMEKLENKLIDMREMYQEHKERVLNGLDEDNAKPQDPFYESQENHNLIGVANIFLEVLFHDVKLDYHTPIISQQGEVAGRLQVEIERIAGQMPQDRMCESVSESSGDSRDEYDDPVDPTSNQITCRVTIKCASGLPLSLSNFVFCQYTFWGHQEMVVPVINAESTAHDQNMVFKFEHTQDFTVTINEEFLEHCIEGALSIEVWGHRSAGFSKTKGWEVEQQQAKARSLVDRWAELSRKIELWVEIHELNDNGEYSPVEVTNRNEVLTGGIYQLRQGQQRRVNVRVKPVQNSGTLPIICQSIVNVAIGSVTVRSRLQRPLDSYQEEDLTVLREKWSEALGRRRQYLDQQIQMLIKKEEKNEQERERELSLVHQWVSLTEERNAVLVPAPGSGIPGAPASWEPPSGMEPHVPVLFLNLNGDDLSAQNTNDELSVAGINSILSKEHGHKFYTLQILQHLDKDVCCVASWDSSMHDSQALNRVTEANERVYLILRTTVRLSHPAPMDLVLRKRLSINIKKGQTLTDRLKKFRLVRGENAIWQSGVTYEVVSNIPKASEELEDRESLAQLAASGDDCSASDGETYIEKYTRGVSAVESILTLDRLRQNVAVKELETAHGQPLSMRKTVSVPNFSQAVKDTTNTGSQLINKLTQIMRFDASMESLLNVGRSESFADLNNSALGNKFTPGHSPAGAGGVIRSRHSFGGKGSSDDSPGKAFGIARPTFLNLNLNLNTLRIAPTKPSPATSKLLGMRMTTLHEEPLGGHRSLDEEPEDSYSDSEYAAEYEQERQQNKSMATRSRLTASKTMDSFMDVSSHSNQSYLSYTSSANANMKHLTGLATLSMSSSTSSGYGSQAVSCNNLSNEDIASMRSMSIDETPDFDRVNSNSPPNRQARVNPFLKDMPKAKIQEQPEQQAKKLQEAFTHPLEQLESSQNAQSDDDECAQLPKNNNNNLDLVNEPKPLSSQTDLEESMSQPKSKTEFATDNQNGNRSSDELSHSSEDLLEGDGIVREELPAGKVVRRKKSNTQPPSNGNSINNNNNGTTQAPRINHRASVAKMEGLAAYLDSSIMTSSTEVDEESKDVELVLPEWIVVGESVLIRPYNTSGVIRFVGTTEFQPGAWIGVELDTPTGKNDGSVKGVQYFQCKPKHGMFVRSDKLMLDKRGKAMRAYKAAEKSNSISKEMSTSMTGSMTRSKSRGDSLNLSARK

Target ORF information:

RefSeq Version	NM_001274070.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin heavy chain 73, transcript variant D (Khc-73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274070.2

ORF Insert Sequence:

ATGGAAAAAC AGCAGACGAT ACTGCAGAAT CCGCCGCCAC TGGAAAAAAT AGAGAGAAAA 
CAACCAAAGA CATTCGCATT CGATCACTGC TTTTACTCAT TGAACCCCGA GGACGAGAAC 
TTTGCGTCCC AGGAGACAGT GTTCGATTGC GTGGGACGTG GAATTCTGGA TAATGCATTC 
CAGGGCTATA ATGCGTGCAT ATTCGCTTAC GGCCAGACAG GTTCTGGCAA GTCCTACACG 
ATGATGGGCA CCCAGGAGAG CAAGGGCATC ATTCCACGTC TGTGTGACCA ACTCTTCTCG 
GCCATAGCCA ACAAATCCAC ACCCGAGCTT ATGTACAAGG TGGAGGTGTC CTACATGGAG 
ATCTATAACG AGAAGGTCCA CGATCTGCTC GATCCCAAGC CGAACAAACA GTCGCTTAAG 
GTGCGCGAGC ACAATGTTAT GGGCCCATAT GTGGACGGAC TGTCGCAGCT GGCTGTGACA 
TCCTATCAGG ACATCGATAA CCTCATGACC GAAGGCAACA AGTCACGAAC GGTGGCCGCC 
ACCAATATGA ATGCCGAGTC GTCGCGCTCC CACGCCGTCT TCTCAGTGGT CCTCACTCAG 
ATACTCACGG ATCAGGCGAC GGGAGTGAGC GGCGAGAAGG TGTCCCGCAT GTCCCTGGTG 
GATTTGGCTG GCTCCGAGCG GGCTGTGAAA ACGGGAGCTG TTGGCGATCG TCTCAAGGAA 
GGCTCTAACA TCAACAAATC TCTGACCACA CTTGGCCTGG TCATCTCCAA GTTGGCCGAT 
CAATCCAATG GCAAGAAGAG CGGTAACGAC AAATTCGTTC CCTACCGCGA TTCCGTGCTC 
ACCTGGCTGC TGAAAGACAA TTTGGGTGGC AATTCCAGAA CTGTTATGGT AGCGACAATC 
TCGCCATCGG CAGATAACTA CGAGGAGACG CTTTCCACGC TGCGTTATGC AGATCGAGCC 
AAGCGCATTG TTAACCACGC TGTTGTCAAC GAAGATCCCA ATGCCCGCAT CATTCGTGAG 
CTGCGACACG AGGTGGAGAC CCTTAGAAGT ATGCTGAAAC ACGCCACTGG TTCACCAGTG 
GGCGATGTCC AGGATAAGCT GGCTGAGAGC GAGAACCTGA TGAAACAGAT TTCGCAGACT 
TGGGAGGAGA AGCTGGTTAA GACAGAACGC ATTCAAAACG AACGGCAGCA GGCTCTAGAG 
AAAATGGGCA TTAGTGTACA GGCCAGTGGC ATCAAGGTAG AGAAGAACAA GTACTACTTG 
GTCAATTTAA ATGCTGATCC GTCCCTTAAC GAGCTGCTGG TCTATTATCT GAAGGATCGC 
ACACTGATTG GCGGACGCAC TATCAGTGGC CAGCAGCCGG ATATACAACT TTCCGGTCTC 
GGAATCCAGC CCGAGCACTG TGTGATCACT ATCGAGGATA GTGGACTGTA CATGGAGCCA 
GTGCAGGGAG CGCGTTGCTT TGTGAACGGA TCTGCTGCTG TGGAAAAGAC ACCACTCCAG 
AACGGCGACC GTATCCTGTG GGGCAACCAC CACTTTTTCC GCGTCAACTC GCCGAAGAGC 
AATAACACTA GTATGTGCGC TTCGGAGCCC CAGACGCCGG CGCAACTGAT TGATTACAAT 
TTCGCACGCG ATGAGATTAT GCAGAACGAA TTGAGCAACG ATCCTATCCA GACGGCCATT 
GCTCGGCTGG AACGTCAGCA CGAGGAAGAT AAGCAGGTGG CGCTTGAAAA ACAACGGCAG 
GAGTACGAGC GTCAGTTCCA GCAGCTACGC AATATTCTGT CGCCTAGTAC ACCATATGCA 
CCTTATGCTC CTTATGATCC ACTGCGCATG GGTAAGATTA CCCCAAATAC TCCTACTTCG 
CAAATGCGAG TGGAAAAGTG GGCACAGGAA CGAGATGAGA TGTTTCGTCG CAGTTTGGGT 
CAGCTGAAAA CGGATATTAT GCGTGCGAAT TCCCTGGTCC AGGAGGCCAA CTTTCTGGCT 
GAGGAGATGG AGAAGAAGAC CAAGTTTTCA GTCACTCTGC AGATTCCGCC AGCTAATCTA 
AGCCCTAACA GAAGGCGTGG GGCATTTGTC AGCGAACCCG CCATTCTGGT GAAGCGCACA 
AACTCTGGTA GCCAGATCTG GACGATGGAG AAGCTGGAAA ACAAGCTGAT AGACATGCGC 
GAGATGTACC AGGAGCACAA GGAGCGCGTT CTGAACGGAC TGGACGAGGA TAATGCCAAG 
CCACAGGATC CGTTTTACGA GTCGCAAGAG AACCACAATC TTATTGGCGT GGCCAATATA 
TTCTTGGAGG TTCTGTTTCA TGACGTCAAG CTGGACTACC ACACGCCCAT CATTAGCCAG 
CAAGGCGAGG TAGCGGGTCG GCTTCAGGTT GAGATCGAGC GGATTGCCGG CCAGATGCCA 
CAGGACCGCA TGTGCGAGTC AGTTTCAGAA TCCTCTGGCG ATTCACGGGA TGAGTACGAC 
GACCCTGTGG ATCCCACATC GAATCAGATT ACCTGCCGTG TGACGATCAA GTGCGCCAGT 
GGACTGCCAT TGTCGCTCTC CAACTTTGTC TTTTGCCAGT ACACTTTTTG GGGTCACCAA 
GAGATGGTTG TTCCGGTTAT CAATGCTGAA TCAACGGCGC ACGACCAGAA CATGGTGTTT 
AAGTTTGAAC ACACCCAGGA CTTCACGGTT ACCATAAACG AAGAGTTTTT GGAGCACTGC 
ATAGAAGGAG CTCTGTCCAT TGAGGTGTGG GGCCATCGCA GTGCCGGCTT CTCCAAGACA 
AAGGGCTGGG AAGTCGAACA GCAGCAAGCC AAGGCCCGTT CCCTGGTCGA TCGCTGGGCG 
GAGTTGTCGC GGAAGATCGA GCTTTGGGTG GAGATTCACG AGCTTAATGA CAACGGCGAG 
TATTCGCCAG TGGAGGTTAC CAATCGCAAC GAGGTCTTGA CCGGTGGCAT TTACCAGTTA 
CGTCAGGGTC AGCAGCGGCG GGTGAATGTA CGGGTGAAGC CGGTGCAAAA CTCTGGCACC 
TTGCCCATTA TCTGCCAGTC GATTGTGAAC GTTGCCATTG GCAGTGTGAC AGTGCGATCT 
CGGTTGCAGC GACCACTGGA TTCTTACCAG GAGGAAGATC TCACCGTGCT GCGCGAGAAG 
TGGAGCGAAG CACTGGGACG AAGGCGTCAA TACCTTGACC AACAGATCCA GATGCTTATA 
AAGAAAGAGG AGAAGAACGA ACAGGAGCGC GAGAGGGAGC TAAGCTTGGT TCATCAGTGG 
GTCTCGTTGA CGGAGGAACG CAACGCGGTG TTGGTGCCAG CTCCTGGCTC GGGCATTCCC 
GGAGCACCTG CCTCATGGGA GCCGCCGTCC GGAATGGAGC CACATGTGCC CGTCCTCTTC 
CTCAACCTGA ACGGTGACGA TCTGTCGGCG CAAAACACCA ACGACGAGCT CTCCGTCGCC 
GGCATCAATT CCATTCTCTC CAAGGAGCAC GGACATAAGT TTTACACGCT GCAAATTCTG 
CAGCACCTAG ATAAGGATGT TTGCTGTGTG GCCAGCTGGG ACTCTTCGAT GCATGACAGT 
CAGGCCCTGA ACCGTGTCAC TGAGGCCAAT GAGCGGGTCT ATCTCATTCT GCGCACAACG 
GTACGCCTCT CGCACCCAGC ACCTATGGAT CTCGTGCTCC GCAAGCGACT AAGCATTAAC 
ATCAAGAAGG GACAGACGCT AACCGACCGC CTGAAGAAAT TCCGTCTTGT GCGGGGAGAG 
AACGCCATCT GGCAGAGCGG TGTCACCTAT GAGGTGGTCT CTAACATTCC AAAAGCCTCC 
GAGGAGTTGG AGGACCGCGA GTCTCTTGCA CAGTTGGCTG CCAGCGGAGA TGATTGCTCC 
GCAAGTGACG GCGAAACCTA CATAGAGAAA TACACGCGCG GTGTTTCGGC GGTGGAGAGC 
ATACTGACTC TGGATAGACT GCGGCAGAAT GTGGCGGTCA AGGAGCTGGA AACGGCACAT 
GGACAGCCGC TCAGCATGCG CAAGACCGTC AGCGTGCCGA ACTTCTCACA GGCGGTCAAA 
GACACCACCA ATACCGGGAG TCAACTCATA AATAAACTTA CGCAGATTAT GCGCTTCGAT 
GCATCGATGG AGTCGCTGCT GAATGTGGGA CGATCCGAGT CCTTTGCCGA TCTCAATAAC 
AGCGCTTTGG GCAACAAGTT TACTCCAGGT CATAGTCCAG CAGGAGCAGG CGGAGTCATC 
CGAAGTCGCC ACAGCTTTGG AGGCAAAGGA AGCAGCGATG ATTCTCCCGG AAAAGCCTTT 
GGCATTGCGC GTCCAACATT TTTGAATCTC AATTTGAACT TGAACACTTT GAGAATTGCG 
CCAACGAAAC CTTCGCCAGC TACTAGTAAG CTGCTGGGCA TGCGCATGAC TACGTTGCAC 
GAGGAGCCAC TGGGTGGACA CAGATCTCTC GACGAAGAGC CTGAGGACAG CTACAGCGAC 
TCGGAATATG CTGCCGAGTA CGAACAGGAG CGGCAGCAGA ACAAAAGCAT GGCCACGCGA 
TCCCGCCTCA CGGCTTCCAA GACCATGGAC TCATTCATGG ACGTCAGCAG CCATTCGAAC 
CAAAGCTACT TGAGCTACAC GTCCAGTGCC AACGCTAACA TGAAGCACCT GACCGGCCTG 
GCGACTTTGA GCATGAGCTC ATCCACCAGC AGTGGCTACG GTTCCCAGGC TGTCTCCTGC 
AATAATCTGA GCAACGAGGA TATTGCTTCA ATGCGTTCCA TGAGCATTGA TGAGACACCA 
GATTTCGATC GCGTTAACTC GAATTCGCCA CCAAACCGGC AGGCACGAGT TAACCCCTTT 
CTCAAGGACA TGCCCAAAGC TAAAATACAA GAGCAACCGG AACAGCAGGC CAAGAAGCTG 
CAGGAAGCAT TTACGCATCC GTTGGAGCAG CTGGAGTCCA GTCAAAACGC ACAAAGCGAC 
GATGATGAAT GCGCGCAACT GCCAAAGAAT AATAACAACA ACTTGGACTT GGTAAACGAG 
CCGAAGCCAC TTTCAAGCCA AACGGATCTG GAGGAATCTA TGTCGCAACC TAAATCAAAA 
ACGGAGTTTG CCACAGACAA TCAGAACGGC AACAGGTCCT CTGACGAGTT AAGCCACAGT 
TCCGAGGATC TGCTCGAAGG CGATGGCATC GTCCGGGAAG AGTTGCCCGC TGGAAAGGTG 
GTGCGGCGAA AGAAGTCCAA TACCCAGCCC CCGAGCAATG GCAACAGCAT AAATAACAAT 
AACAACGGCA CAACCCAGGC ACCGCGCATC AATCATCGGG CATCGGTGGC CAAAATGGAG 
GGTCTGGCTG CATATTTGGA TTCTAGCATT ATGACCAGCA GCACCGAAGT TGATGAGGAG 
AGCAAGGATG TGGAATTGGT TCTGCCCGAG TGGATAGTGG TGGGCGAGTC AGTGTTAATC 
CGGCCGTATA ACACCAGCGG CGTCATTCGC TTTGTGGGAA CCACGGAATT CCAACCCGGT 
GCCTGGATTG GCGTTGAATT GGACACACCA ACGGGCAAAA ACGACGGCAG CGTGAAGGGC 
GTTCAGTATT TCCAGTGCAA GCCCAAGCAC GGCATGTTTG TGCGCTCCGA CAAGCTGATG 
CTGGACAAGC GTGGCAAGGC GATGCGAGCC TACAAGGCCG CCGAGAAGAG CAACAGCATC 
AGCAAAGAGA TGAGTACCTC GATGACTGGC TCGATGACAC GCTCCAAGAG CCGCGGCGAT 
TCGCTAAACC TTTCGGCGCG TAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Khc-73 ( NM_001274071.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261000.1 Drosophila melanogaster kinesin heavy chain 73, transcript variant E (Khc-73), mRNA.

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CloneID	OFa12067
Clone ID Related Accession (Same CDS sequence)	NM_001274071.2
Accession Version	NM_001274071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin heavy chain 73, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274071.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGG ACGAGGATAA TGCCAAGCCA CAGGATCCGT TTTACGAGTC GCAAGAGAAC 
CACAATCTTA TTGGCGTGGC CAATATATTC TTGGAGGTTC TGTTTCATGA CGTCAAGCTG 
GACTACCACA CGCCCATCAT TAGCCAGCAA GGCGAGGTAG CGGGTCGGCT TCAGGTTGAG 
ATCGAGCGGA TTGCCGGCCA GATGCCACAG GACCGCATGT GCGAGTCAGT TTCAGAATCC 
TCTGGCGATT CACGGGATGA GTACGACGAC CCTGTGGATC CCACATCGAA TCAGATTACC 
TGCCGTGTGA CGATCAAGTG CGCCAGTGGA CTGCCATTGT CGCTCTCCAA CTTTGTCTTT 
TGCCAGTACA CTTTTTGGGG TCACCAAGAG ATGGTTGTTC CGGTTATCAA TGCTGAATCA 
ACGGCGCACG ACCAGAACAT GGTGTTTAAG TTTGAACACA CCCAGGACTT CACGGTTACC 
ATAAACGAAG AGTTTTTGGA GCACTGCATA GAAGGAGCTC TGTCCATTGA GGTGTGGGGC 
CATCGCAGTG CCGGCTTCTC CAAGACAAAG GGCTGGGAAG TCGAACAGCA GCAAGCCAAG 
GCCCGTTCCC TGGTCGATCG CTGGGCGGAG TTGTCGCGGA AGATCGAGCT TTGGGTGGAG 
ATTCACGAGC TTAATGACAA CGGCGAGTAT TCGCCAGTGG AGGTTACCAA TCGCAACGAG 
GTCTTGACCG GTGGCATTTA CCAGTTACGT CAGGGTCAGC AGCGGCGGGT GAATGTACGG 
GTGAAGCCGG TGCAAAACTC TGGCACCTTG CCCATTATCT GCCAGTCGAT TGTGAACGTT 
GCCATTGGCA GTGTGACAGT GCGATCTCGG TTGCAGCGAC CACTGGATTC TTACCAGGAG 
GAAGATCTCA CCGTGCTGCG CGAGAAGTGG AGCGAAGCAC TGGGACGAAG GCGTCAATAC 
CTTGACCAAC AGATCCAGAT GCTTATAAAG AAAGAGGAGA AGAACGAACA GGAGCGCGAG 
AGGGAGCTAA GCTTGGTTCA TCAGTGGGTC TCGTTGACGG AGGAACGCAA CGCGGTGTTG 
GTGCCAGCTC CTGGCTCGGG CATTCCCGGA GCACCTGCCT CATGGGAGCC GCCGTCCGGA 
ATGGAGCCAC ATGTGCCCGT CCTCTTCCTC AACCTGAACG GTGACGATCT GTCGGCGCAA 
AACACCAACG ACGAGCTCTC CGTCGCCGGC ATCAATTCCA TTCTCTCCAA GGAGCACGGA 
CATAAGTTTT ACACGCTGCA AATTCTGCAG CACCTAGATA AGGATGTTTG CTGTGTGGCC 
AGCTGGGACT CTTCGATGCA TGACAGTCAG GCCCTGAACC GTGTCACTGA GGCCAATGAG 
CGGGTCTATC TCATTCTGCG CACAACGGTA CGCCTCTCGC ACCCAGCACC TATGGATCTC 
GTGCTCCGCA AGCGACTAAG CATTAACATC AAGAAGGGAC AGACGCTAAC CGACCGCCTG 
AAGAAATTCC GTCTTGTGCG GGGAGAGAAC GCCATCTGGC AGAGCGGTGT CACCTATGAG 
GTGGTCTCTA ACATTCCAAA AGCCTCCGAG GAGTTGGAGG ACCGCGAGTC TCTTGCACAG 
TTGGCTGCCA GCGGAGATGA TTGCTCCGCA AGTGACGGCG AAACCTACAT AGAGAAATAC 
ACGCGCGGTG TTTCGGCGGT GGAGAGCATA CTGACTCTGG ATAGACTGCG GCAGAATGTG 
GCGGTCAAGG AGCTGGAAAC GGCACATGGA CAGCCGCTCA GCATGCGCAA GACCGTCAGC 
GTGCCGAACT TCTCACAGAT TATGCGCTTC GATGCATCGA TGGAGTCGCT GCTGAATGTG 
GGACGATCCG AGTCCTTTGC CGATCTCAAT AACAGCGCTT TGGGCAACAA GTTTACTCCA 
GCAGGTCATA GTCCAGCAGG AGCAGGCGGA GTCATCCGAA GTCGCCACAG CTTTGGAGGC 
AAAGGAAGCA GCGATGATTC TCCCGGAAAA GCCTTTGGCA TTGCTTCGCC AGCTACTAGT 
AAGCTGCTGG GCATGCGCAT GACTACGTTG CACGAGGAGC CACTGGGTGG ACACAGATCT 
CTCGACGAAG AGCCTGAGGA CAGCTACAGC GACTCGGAAT ATGCTGCCGA GTACGAACAG 
GAGCGGCAGC AGAACAAAAG CATGGCCACG CGATCCCGCC TCACGGCTTC CAAGACCATG 
GACTCATTCA TGGACGTCAG CAGCCATTCG AACCAAAGCT ACTTGAGCTA CACGTCCAGT 
GCCAACGCTA ACATGAAGCA CCTGACCGGC CTGGCGACTT TGAGCATGAG CTCATCCACC 
AGCAGTGGCT ACGGTTCCCA GGCTGTCTCC TGCAATAATC TGAGCAACGA GGATATTGCT 
TCAATGCGTT CCATGAGCAT TGATGAGACA CCAGATTTCG ATCGCGTTAA CTCGAATTCG 
CCACCAAACC GGCAGGCACG AGTTAACCCC TTTCTCAAGG ACATGCCCAA AGCTAAAATA 
CAAGAGCAAC CGGAACAGCA GGCCAAGAAG CTGCAGGAAG CATTTACGCA TCCGTTGGAG 
CAGCTGGAGT CCAGTCAAAA CGCACAAAGC GACGATGATG AATGCGCGCA ACTGCCAAAG 
AATAATAACA ACAACTTGGA CTTGGTAAAC GAGCCGAAGC CACTTTCAAG CCAAACGGAT 
CTGGAGGAAT CTATGTCGCA ACCTAAATCA AAAACGGAGT TTGCCACAGA CAATCAGAAC 
GGCAACAGGT CCTCTGACGA GTTAAGCCAC AGTTCCGAGG ATCTGCTCGA AGGCGATGGC 
ATCGTCCGGG AAGAGTTGCC CGCTGGAAAG GTGGTGCGGC GAAAGAAGTC CAATACCCAG 
CCCCCGAGCA ATGGCAACAG CATAAATAAC AATAACAACG GCACAACCCA GGCACCGCGC 
ATCAATCATC GGGCATCGGT GGCCAAAATG GAGGGTCTGG CTGCATATTT GGATTCTAGC 
ATTATGACCA GCAGCACCGA AGTTGATGAG GAGAGCAAGG ATGTGGAATT GGTTCTGCCC 
GAGTGGATAG TGGTGGGCGA GTCAGTGTTA ATCCGGCCGT ATAACACCAG CGGCGTCATT 
CGCTTTGTGG GAACCACGGA ATTCCAACCC GGTGCCTGGA TTGGCGTTGA ATTGGACACA 
CCAACGGGCA AAAACGACGG CAGCGTGAAG GGCGTTCAGT ATTTCCAGTG CAAGCCCAAG 
CACGGCATGT TTGTGCGCTC CGACAAGCTG ATGCTGGACA AGCGTGGCAA GGCGATGCGA 
GCCTACAAGG CCGCCGAGAA GAGCAACAGC ATCAGCAAAG AGATGAGTAC CTCGATGACT 
GGCTCGATGA CACGCTCCAA GAGCCGCGGC GATTCGCTAA ACCTTTCGGC GCGTAAATGA 
TTGTACCCAA AGTGTTCGCA TCAGCTGCAG CGTTGGACTA ATTGCAGGCA ATCGACGGTA 
GCCACCAGCA GCCAGCCGGC GACCTGCCAC AGATTGCGTG CAACCAGCGG CGCTGCAGAT 
GCGAACAGTC ATGGCAAGAA GTGCTATGCC AATCGACGCT CGACGGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261000.1
CDS	345..6218
Translation	MASDKIKVAVRVRPFNRREIELDTKCIVEMEKQQTILQNPPPLEKIERKQPKTFAFDHCFYSLNPEDENFASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGTQESKGIIPRLCDQLFSAIANKSTPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVDGLSQLAVTSYQDIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRMSLVDLAGSERAVKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTWLLKDNLGGNSRTVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVETLRSMLKHATGSPVGDVQDKLAESENLMKQISQTWEEKLVKTERIQNERQQALEKMGISVQASGIKVEKNKYYLVNLNADPSLNELLVYYLKDRTLIGGRTISGQQPDIQLSGLGIQPEHCVITIEDSGLYMEPVQGARCFVNGSAAVEKTPLQNGDRILWGNHHFFRVNSPKSNNTSMCASEPQTPAQLIDYNFARDEIMQNELSNDPIQTAIARLERQHEEDKQVALEKQRQEYERQFQQLRNILSPSTPYAPYAPYDPLRMGKITPNTPTSQMRVEKWAQERDEMFRRSLGQLKTDIMRANSLVQEANFLAEEMEKKTKFSVTLQIPPANLSPNRRRGAFVSEPAILVKRTNSGSQIWTMEKLENKLIDMREMYQEHKERVLNGLDEDNAKPQDPFYESQENHNLIGVANIFLEVLFHDVKLDYHTPIISQQGEVAGRLQVEIERIAGQMPQDRMCESVSESSGDSRDEYDDPVDPTSNQITCRVTIKCASGLPLSLSNFVFCQYTFWGHQEMVVPVINAESTAHDQNMVFKFEHTQDFTVTINEEFLEHCIEGALSIEVWGHRSAGFSKTKGWEVEQQQAKARSLVDRWAELSRKIELWVEIHELNDNGEYSPVEVTNRNEVLTGGIYQLRQGQQRRVNVRVKPVQNSGTLPIICQSIVNVAIGSVTVRSRLQRPLDSYQEEDLTVLREKWSEALGRRRQYLDQQIQMLIKKEEKNEQERERELSLVHQWVSLTEERNAVLVPAPGSGIPGAPASWEPPSGMEPHVPVLFLNLNGDDLSAQNTNDELSVAGINSILSKEHGHKFYTLQILQHLDKDVCCVASWDSSMHDSQALNRVTEANERVYLILRTTVRLSHPAPMDLVLRKRLSINIKKGQTLTDRLKKFRLVRGENAIWQSGVTYEVVSNIPKASEELEDRESLAQLAASGDDCSASDGETYIEKYTRGVSAVESILTLDRLRQNVAVKELETAHGQPLSMRKTVSVPNFSQIMRFDASMESLLNVGRSESFADLNNSALGNKFTPAGHSPAGAGGVIRSRHSFGGKGSSDDSPGKAFGIASPATSKLLGMRMTTLHEEPLGGHRSLDEEPEDSYSDSEYAAEYEQERQQNKSMATRSRLTASKTMDSFMDVSSHSNQSYLSYTSSANANMKHLTGLATLSMSSSTSSGYGSQAVSCNNLSNEDIASMRSMSIDETPDFDRVNSNSPPNRQARVNPFLKDMPKAKIQEQPEQQAKKLQEAFTHPLEQLESSQNAQSDDDECAQLPKNNNNNLDLVNEPKPLSSQTDLEESMSQPKSKTEFATDNQNGNRSSDELSHSSEDLLEGDGIVREELPAGKVVRRKKSNTQPPSNGNSINNNNNGTTQAPRINHRASVAKMEGLAAYLDSSIMTSSTEVDEESKDVELVLPEWIVVGESVLIRPYNTSGVIRFVGTTEFQPGAWIGVELDTPTGKNDGSVKGVQYFQCKPKHGMFVRSDKLMLDKRGKAMRAYKAAEKSNSISKEMSTSMTGSMTRSKSRGDSLNLSARK*LYPKCSHQLQRWTNCRQSTVATSSQPATCHRLRATSGAADANSHGKKCYANRRSTAF

Target ORF information:

RefSeq Version	NM_001274071.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin heavy chain 73, transcript variant E (Khc-73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274071.2

ORF Insert Sequence:

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGG ACGAGGATAA TGCCAAGCCA CAGGATCCGT TTTACGAGTC GCAAGAGAAC 
CACAATCTTA TTGGCGTGGC CAATATATTC TTGGAGGTTC TGTTTCATGA CGTCAAGCTG 
GACTACCACA CGCCCATCAT TAGCCAGCAA GGCGAGGTAG CGGGTCGGCT TCAGGTTGAG 
ATCGAGCGGA TTGCCGGCCA GATGCCACAG GACCGCATGT GCGAGTCAGT TTCAGAATCC 
TCTGGCGATT CACGGGATGA GTACGACGAC CCTGTGGATC CCACATCGAA TCAGATTACC 
TGCCGTGTGA CGATCAAGTG CGCCAGTGGA CTGCCATTGT CGCTCTCCAA CTTTGTCTTT 
TGCCAGTACA CTTTTTGGGG TCACCAAGAG ATGGTTGTTC CGGTTATCAA TGCTGAATCA 
ACGGCGCACG ACCAGAACAT GGTGTTTAAG TTTGAACACA CCCAGGACTT CACGGTTACC 
ATAAACGAAG AGTTTTTGGA GCACTGCATA GAAGGAGCTC TGTCCATTGA GGTGTGGGGC 
CATCGCAGTG CCGGCTTCTC CAAGACAAAG GGCTGGGAAG TCGAACAGCA GCAAGCCAAG 
GCCCGTTCCC TGGTCGATCG CTGGGCGGAG TTGTCGCGGA AGATCGAGCT TTGGGTGGAG 
ATTCACGAGC TTAATGACAA CGGCGAGTAT TCGCCAGTGG AGGTTACCAA TCGCAACGAG 
GTCTTGACCG GTGGCATTTA CCAGTTACGT CAGGGTCAGC AGCGGCGGGT GAATGTACGG 
GTGAAGCCGG TGCAAAACTC TGGCACCTTG CCCATTATCT GCCAGTCGAT TGTGAACGTT 
GCCATTGGCA GTGTGACAGT GCGATCTCGG TTGCAGCGAC CACTGGATTC TTACCAGGAG 
GAAGATCTCA CCGTGCTGCG CGAGAAGTGG AGCGAAGCAC TGGGACGAAG GCGTCAATAC 
CTTGACCAAC AGATCCAGAT GCTTATAAAG AAAGAGGAGA AGAACGAACA GGAGCGCGAG 
AGGGAGCTAA GCTTGGTTCA TCAGTGGGTC TCGTTGACGG AGGAACGCAA CGCGGTGTTG 
GTGCCAGCTC CTGGCTCGGG CATTCCCGGA GCACCTGCCT CATGGGAGCC GCCGTCCGGA 
ATGGAGCCAC ATGTGCCCGT CCTCTTCCTC AACCTGAACG GTGACGATCT GTCGGCGCAA 
AACACCAACG ACGAGCTCTC CGTCGCCGGC ATCAATTCCA TTCTCTCCAA GGAGCACGGA 
CATAAGTTTT ACACGCTGCA AATTCTGCAG CACCTAGATA AGGATGTTTG CTGTGTGGCC 
AGCTGGGACT CTTCGATGCA TGACAGTCAG GCCCTGAACC GTGTCACTGA GGCCAATGAG 
CGGGTCTATC TCATTCTGCG CACAACGGTA CGCCTCTCGC ACCCAGCACC TATGGATCTC 
GTGCTCCGCA AGCGACTAAG CATTAACATC AAGAAGGGAC AGACGCTAAC CGACCGCCTG 
AAGAAATTCC GTCTTGTGCG GGGAGAGAAC GCCATCTGGC AGAGCGGTGT CACCTATGAG 
GTGGTCTCTA ACATTCCAAA AGCCTCCGAG GAGTTGGAGG ACCGCGAGTC TCTTGCACAG 
TTGGCTGCCA GCGGAGATGA TTGCTCCGCA AGTGACGGCG AAACCTACAT AGAGAAATAC 
ACGCGCGGTG TTTCGGCGGT GGAGAGCATA CTGACTCTGG ATAGACTGCG GCAGAATGTG 
GCGGTCAAGG AGCTGGAAAC GGCACATGGA CAGCCGCTCA GCATGCGCAA GACCGTCAGC 
GTGCCGAACT TCTCACAGAT TATGCGCTTC GATGCATCGA TGGAGTCGCT GCTGAATGTG 
GGACGATCCG AGTCCTTTGC CGATCTCAAT AACAGCGCTT TGGGCAACAA GTTTACTCCA 
GCAGGTCATA GTCCAGCAGG AGCAGGCGGA GTCATCCGAA GTCGCCACAG CTTTGGAGGC 
AAAGGAAGCA GCGATGATTC TCCCGGAAAA GCCTTTGGCA TTGCTTCGCC AGCTACTAGT 
AAGCTGCTGG GCATGCGCAT GACTACGTTG CACGAGGAGC CACTGGGTGG ACACAGATCT 
CTCGACGAAG AGCCTGAGGA CAGCTACAGC GACTCGGAAT ATGCTGCCGA GTACGAACAG 
GAGCGGCAGC AGAACAAAAG CATGGCCACG CGATCCCGCC TCACGGCTTC CAAGACCATG 
GACTCATTCA TGGACGTCAG CAGCCATTCG AACCAAAGCT ACTTGAGCTA CACGTCCAGT 
GCCAACGCTA ACATGAAGCA CCTGACCGGC CTGGCGACTT TGAGCATGAG CTCATCCACC 
AGCAGTGGCT ACGGTTCCCA GGCTGTCTCC TGCAATAATC TGAGCAACGA GGATATTGCT 
TCAATGCGTT CCATGAGCAT TGATGAGACA CCAGATTTCG ATCGCGTTAA CTCGAATTCG 
CCACCAAACC GGCAGGCACG AGTTAACCCC TTTCTCAAGG ACATGCCCAA AGCTAAAATA 
CAAGAGCAAC CGGAACAGCA GGCCAAGAAG CTGCAGGAAG CATTTACGCA TCCGTTGGAG 
CAGCTGGAGT CCAGTCAAAA CGCACAAAGC GACGATGATG AATGCGCGCA ACTGCCAAAG 
AATAATAACA ACAACTTGGA CTTGGTAAAC GAGCCGAAGC CACTTTCAAG CCAAACGGAT 
CTGGAGGAAT CTATGTCGCA ACCTAAATCA AAAACGGAGT TTGCCACAGA CAATCAGAAC 
GGCAACAGGT CCTCTGACGA GTTAAGCCAC AGTTCCGAGG ATCTGCTCGA AGGCGATGGC 
ATCGTCCGGG AAGAGTTGCC CGCTGGAAAG GTGGTGCGGC GAAAGAAGTC CAATACCCAG 
CCCCCGAGCA ATGGCAACAG CATAAATAAC AATAACAACG GCACAACCCA GGCACCGCGC 
ATCAATCATC GGGCATCGGT GGCCAAAATG GAGGGTCTGG CTGCATATTT GGATTCTAGC 
ATTATGACCA GCAGCACCGA AGTTGATGAG GAGAGCAAGG ATGTGGAATT GGTTCTGCCC 
GAGTGGATAG TGGTGGGCGA GTCAGTGTTA ATCCGGCCGT ATAACACCAG CGGCGTCATT 
CGCTTTGTGG GAACCACGGA ATTCCAACCC GGTGCCTGGA TTGGCGTTGA ATTGGACACA 
CCAACGGGCA AAAACGACGG CAGCGTGAAG GGCGTTCAGT ATTTCCAGTG CAAGCCCAAG 
CACGGCATGT TTGTGCGCTC CGACAAGCTG ATGCTGGACA AGCGTGGCAA GGCGATGCGA 
GCCTACAAGG CCGCCGAGAA GAGCAACAGC ATCAGCAAAG AGATGAGTAC CTCGATGACT 
GGCTCGATGA CACGCTCCAA GAGCCGCGGC GATTCGCTAA ACCTTTCGGC GCGTAAATGA 
TTGTACCCAA AGTGTTCGCA TCAGCTGCAG CGTTGGACTA ATTGCAGGCA ATCGACGGTA 
GCCACCAGCA GCCAGCCGGC GACCTGCCAC AGATTGCGTG CAACCAGCGG CGCTGCAGAT 
GCGAACAGTC ATGGCAAGAA GTGCTATGCC AATCGACGCT CGACGGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Khc-73 ( NM_001299533.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286462.1 Drosophila melanogaster kinesin heavy chain 73, transcript variant F (Khc-73), mRNA.

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CloneID	OFa21824
Clone ID Related Accession (Same CDS sequence)	NM_001299533.1
Accession Version	NM_001299533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin heavy chain 73, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGC CCCTTATAGA GCCATTCTCA GAAGACGAGT TCGATGACAA GGACGAGGAT 
AATGCCAAGC CACAGGATCC GTTTTACGAG TCGCAAGAGA ACCACAATCT TATTGGCGTG 
GCCAATATAT TCTTGGAGGT TCTGTTTCAT GACGTCAAGC TGGACTACCA CACGCCCATC 
ATTAGCCAGC AAGGCGAGGT AGCGGGTCGG CTTCAGGTTG AGATCGAGCG GATTGCCGGC 
CAGATGCCAC AGGACCGCAT GTGCGAGTCA GTTTCAGAAT CCTCTGGCGA TTCACGGGAT 
GAGTACGACG ACCCTGTGGA TCCCACATCG AATCAGATTA CCTGCCGTGT GACGATCAAG 
TGCGCCAGTG GACTGCCATT GTCGCTCTCC AACTTTGTCT TTTGCCAGTA CACTTTTTGG 
GGTCACCAAG AGATGGTTGT TCCGGTTATC AATGCTGAAT CAACGGCGCA CGACCAGAAC 
ATGGTGTTTA AGTTTGAACA CACCCAGGAC TTCACGGTTA CCATAAACGA AGAGTTTTTG 
GAGCACTGCA TAGAAGGAGC TCTGTCCATT GAGGTGTGGG GCCATCGCAG TGCCGGCTTC 
TCCAAGACAA AGGGCTGGGA AGTCGAACAG CAGCAAGCCA AGGCCCGTTC CCTGGTCGAT 
CGCTGGGCGG AGTTGTCGCG GAAGATCGAG CTTTGGGTGG AGATTCACGA GCTTAATGAC 
AACGGCGAGT ATTCGCCAGT GGAGGTTACC AATCGCAACG AGGTCTTGAC CGGTGGCATT 
TACCAGTTAC GTCAGGGTCA GCAGCGGCGG GTGAATGTAC GGGTGAAGCC GGTGCAAAAC 
TCTGGCACCT TGCCCATTAT CTGCCAGTCG ATTGTGAACG TTGCCATTGG CAGTGTGACA 
GTGCGATCTC GGTTGCAGCG ACCACTGGAT TCTTACCAGG AGGAAGATCT CACCGTGCTG 
CGCGAGAAGT GGAGCGAAGC ACTGGGACGA AGGCGTCAAT ACCTTGACCA ACAGATCCAG 
ATGCTTATAA AGAAAGAGGA GAAGAACGAA CAGGAGCGCG AGAGGGAGCT AAGCTTGGTT 
CATCAGTGGG TCTCGTTGAC GGAGGAACGC AACGCGGTGT TGGTGCCAGC TCCTGGCTCG 
GGCATTCCCG GAGCACCTGC CTCATGGGAG CCGCCGTCCG GAATGGAGCC ACATGTGCCC 
GTCCTCTTCC TCAACCTGAA CGGTGACGAT CTGTCGGCGC AAAACACCAA CGACGAGCTC 
TCCGTCGCCG GCATCAATTC CATTCTCTCC AAGGAGCACG GACATAAGTT TTACACGCTG 
CAAATTCTGC AGCACCTAGA TAAGGATGTT TGCTGTGTGG CCAGCTGGGA CTCTTCGATG 
CATGACAGTC AGGCCCTGAA CCGTGTCACT GAGGCCAATG AGCGGGTCTA TCTCATTCTG 
CGCACAACGG TACGCCTCTC GCACCCAGCA CCTATGGATC TCGTGCTCCG CAAGCGACTA 
AGCATTAACA TCAAGAAGGG ACAGACGCTA ACCGACCGCC TGAAGAAATT CCGTCTTGTG 
CGGGGAGAGA ACGCCATCTG GCAGAGCGGT GTCACCTATG AGGTGGTCTC TAACATTCCA 
AAAGCCTCCG AGGAGTTGGA GGACCGCGAG TCTCTTGCAC AGTTGGCTGC CAGCGGAGAT 
GATTGCTCCG CAAGTGACGG CGAAACCTAC ATAGAGAAAT ACACGCGCGG TGTTTCGGCG 
GTGGAGAGCA TACTGACTCT GGATAGACTG CGGCAGAATG TGGCGGTCAA GGAGCTGGAA 
ACGGCACATG GACAGCCGCT CAGCATGCGC AAGACCGTCA GCGTGCCGAA CTTCTCACAG 
ATTATGCGCT TCGATGCATC GATGGAGTCG CTGCTGAATG TGGGACGATC CGAGTCCTTT 
GCCGATCTCA ATAACAGCGC TTTGGGCAAC AAGTTTACTC CAGCAGGTCA TAGTCCAGCA 
GGAGCAGGCG GAGTCATCCG AAGTCGCCAC AGCTTTGGAG GCAAAGGAAG CAGCGATGAT 
TCTCCCGGAA AAGCCTTTGG CATTGCTTCG CCAGCTACTA GTAAGCTGCT GGGCATGCGC 
ATGACTACGT TGCACGAGGA GCCACTGGGT GGACACAGAT CTCTCGACGA AGAGCCTGAG 
GACAGCTACA GCGACTCGGA ATATGCTGCC GAGTACGAAC AGGAGCGGCA GCAGAACAAA 
AGCATGGCCA CGCGATCCCG CCTCACGGCT TCCAAGACCA TGGACTCATT CATGGACGTC 
AGCAGCCATT CGAACCAAAG CTACTTGAGC TACACGTCCA GTGCCAACGC TAACATGAAG 
CACCTGACCG GCCTGGCGAC TTTGAGCATG AGCTCATCCA CCAGCAGTGG CTACGGTTCC 
CAGGCTGTCT CCTGCAATAA TCTGAGCAAC GAGGATATTG CTTCAATGCG TTCCATGAGC 
ATTGATGAGA CACCAGATTT CGATCGCGTT AACTCGAATT CGCCACCAAA CCGGCAGGCA 
CGAGTTAACC CCTTTCTCAA GGACATGCCC AAAGCTAAAA TACAAGAGCA ACCGGAACAG 
CAGGCCAAGA AGCTGCAGGA AGCATTTACG CATCCGTTGG AGCAGCTGGA GTCCAGTCAA 
AACGCACAAA GCGACGATGA TGAATGCGCG CAACTGCCAA AGAATAATAA CAACAACTTG 
GACTTGGTAA ACGAGCCGAA GCCACTTTCA AGCCAAACGG ATCTGGAGGA ATCTATGTCG 
CAACCTAAAT CAAAAACGGA GTTTGCCACA GACAATCAGA ACGGCAACAG GTCCTCTGAC 
GAGTTAAGCC ACAGTTCCGA GGATCTGCTC GAAGGCGATG GCATCGTCCG GGAAGAGTTG 
CCCGCTGGAA AGGTGGTGCG GCGAAAGAAG TCCAATACCC AGCCCCCGAG CAATGGCAAC 
AGCATAAATA ACAATAACAA CGGCACAACC CAGGCACCGC GCATCAATCA TCGGGCATCG 
GTGGCCAAAA TGGAGGGTCT GGCTGCATAT TTGGATTCTA GCATTATGAC CAGCAGCACC 
GAAGTTGATG AGGAGAGCAA GGATGTGGAA TTGGTTCTGC CCGAGTGGAT AGTGGTGGGC 
GAGTCAGTGT TAATCCGGCC GTATAACACC AGCGGCGTCA TTCGCTTTGT GGGAACCACG 
GAATTCCAAC CCGGTGCCTG GATTGGCGTT GAATTGGACA CACCAACGGG CAAAAACGAC 
GGCAGCGTGA AGGGCGTTCA GTATTTCCAG TGCAAGCCCA AGCACGGCAT GTTTGTGCGC 
TCCGACAAGC TGATGCTGGA CAAGCGTGGC AAGGCGATGC GAGCCTACAA GGCCGCCGAG 
AAGAGCAACA GCATCAGCAA AGAGATGAGT ACCTCGATGA CTGGCTCGAT GACACGCTCC 
AAGAGCCGCG GCGATTCGCT AAACCTTTCG GCGCGTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286462.1
CDS	345..6086
Translation	MASDKIKVAVRVRPFNRREIELDTKCIVEMEKQQTILQNPPPLEKIERKQPKTFAFDHCFYSLNPEDENFASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGTQESKGIIPRLCDQLFSAIANKSTPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVDGLSQLAVTSYQDIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRMSLVDLAGSERAVKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTWLLKDNLGGNSRTVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVETLRSMLKHATGSPVGDVQDKLAESENLMKQISQTWEEKLVKTERIQNERQQALEKMGISVQASGIKVEKNKYYLVNLNADPSLNELLVYYLKDRTLIGGRTISGQQPDIQLSGLGIQPEHCVITIEDSGLYMEPVQGARCFVNGSAAVEKTPLQNGDRILWGNHHFFRVNSPKSNNTSMCASEPQTPAQLIDYNFARDEIMQNELSNDPIQTAIARLERQHEEDKQVALEKQRQEYERQFQQLRNILSPSTPYAPYAPYDPLRMGKITPNTPTSQMRVEKWAQERDEMFRRSLGQLKTDIMRANSLVQEANFLAEEMEKKTKFSVTLQIPPANLSPNRRRGAFVSEPAILVKRTNSGSQIWTMEKLENKLIDMREMYQEHKERVLNGLPLIEPFSEDEFDDKDEDNAKPQDPFYESQENHNLIGVANIFLEVLFHDVKLDYHTPIISQQGEVAGRLQVEIERIAGQMPQDRMCESVSESSGDSRDEYDDPVDPTSNQITCRVTIKCASGLPLSLSNFVFCQYTFWGHQEMVVPVINAESTAHDQNMVFKFEHTQDFTVTINEEFLEHCIEGALSIEVWGHRSAGFSKTKGWEVEQQQAKARSLVDRWAELSRKIELWVEIHELNDNGEYSPVEVTNRNEVLTGGIYQLRQGQQRRVNVRVKPVQNSGTLPIICQSIVNVAIGSVTVRSRLQRPLDSYQEEDLTVLREKWSEALGRRRQYLDQQIQMLIKKEEKNEQERERELSLVHQWVSLTEERNAVLVPAPGSGIPGAPASWEPPSGMEPHVPVLFLNLNGDDLSAQNTNDELSVAGINSILSKEHGHKFYTLQILQHLDKDVCCVASWDSSMHDSQALNRVTEANERVYLILRTTVRLSHPAPMDLVLRKRLSINIKKGQTLTDRLKKFRLVRGENAIWQSGVTYEVVSNIPKASEELEDRESLAQLAASGDDCSASDGETYIEKYTRGVSAVESILTLDRLRQNVAVKELETAHGQPLSMRKTVSVPNFSQIMRFDASMESLLNVGRSESFADLNNSALGNKFTPAGHSPAGAGGVIRSRHSFGGKGSSDDSPGKAFGIASPATSKLLGMRMTTLHEEPLGGHRSLDEEPEDSYSDSEYAAEYEQERQQNKSMATRSRLTASKTMDSFMDVSSHSNQSYLSYTSSANANMKHLTGLATLSMSSSTSSGYGSQAVSCNNLSNEDIASMRSMSIDETPDFDRVNSNSPPNRQARVNPFLKDMPKAKIQEQPEQQAKKLQEAFTHPLEQLESSQNAQSDDDECAQLPKNNNNNLDLVNEPKPLSSQTDLEESMSQPKSKTEFATDNQNGNRSSDELSHSSEDLLEGDGIVREELPAGKVVRRKKSNTQPPSNGNSINNNNNGTTQAPRINHRASVAKMEGLAAYLDSSIMTSSTEVDEESKDVELVLPEWIVVGESVLIRPYNTSGVIRFVGTTEFQPGAWIGVELDTPTGKNDGSVKGVQYFQCKPKHGMFVRSDKLMLDKRGKAMRAYKAAEKSNSISKEMSTSMTGSMTRSKSRGDSLNLSARK

Target ORF information:

RefSeq Version	NM_001299533.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin heavy chain 73, transcript variant F (Khc-73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299533.1

ORF Insert Sequence:

ATGGCTAGTG ATAAGATCAA AGTCGCCGTA AGGGTGCGAC CCTTCAATCG CCGAGAAATC 
GAACTAGATA CGAAATGTAT CGTGGAAATG GAAAAACAGC AGACGATACT GCAGAATCCG 
CCGCCACTGG AAAAAATAGA GAGAAAACAA CCAAAGACAT TCGCATTCGA TCACTGCTTT 
TACTCATTGA ACCCCGAGGA CGAGAACTTT GCGTCCCAGG AGACAGTGTT CGATTGCGTG 
GGACGTGGAA TTCTGGATAA TGCATTCCAG GGCTATAATG CGTGCATATT CGCTTACGGC 
CAGACAGGTT CTGGCAAGTC CTACACGATG ATGGGCACCC AGGAGAGCAA GGGCATCATT 
CCACGTCTGT GTGACCAACT CTTCTCGGCC ATAGCCAACA AATCCACACC CGAGCTTATG 
TACAAGGTGG AGGTGTCCTA CATGGAGATC TATAACGAGA AGGTCCACGA TCTGCTCGAT 
CCCAAGCCGA ACAAACAGTC GCTTAAGGTG CGCGAGCACA ATGTTATGGG CCCATATGTG 
GACGGACTGT CGCAGCTGGC TGTGACATCC TATCAGGACA TCGATAACCT CATGACCGAA 
GGCAACAAGT CACGAACGGT GGCCGCCACC AATATGAATG CCGAGTCGTC GCGCTCCCAC 
GCCGTCTTCT CAGTGGTCCT CACTCAGATA CTCACGGATC AGGCGACGGG AGTGAGCGGC 
GAGAAGGTGT CCCGCATGTC CCTGGTGGAT TTGGCTGGCT CCGAGCGGGC TGTGAAAACG 
GGAGCTGTTG GCGATCGTCT CAAGGAAGGC TCTAACATCA ACAAATCTCT GACCACACTT 
GGCCTGGTCA TCTCCAAGTT GGCCGATCAA TCCAATGGCA AGAAGAGCGG TAACGACAAA 
TTCGTTCCCT ACCGCGATTC CGTGCTCACC TGGCTGCTGA AAGACAATTT GGGTGGCAAT 
TCCAGAACTG TTATGGTAGC GACAATCTCG CCATCGGCAG ATAACTACGA GGAGACGCTT 
TCCACGCTGC GTTATGCAGA TCGAGCCAAG CGCATTGTTA ACCACGCTGT TGTCAACGAA 
GATCCCAATG CCCGCATCAT TCGTGAGCTG CGACACGAGG TGGAGACCCT TAGAAGTATG 
CTGAAACACG CCACTGGTTC ACCAGTGGGC GATGTCCAGG ATAAGCTGGC TGAGAGCGAG 
AACCTGATGA AACAGATTTC GCAGACTTGG GAGGAGAAGC TGGTTAAGAC AGAACGCATT 
CAAAACGAAC GGCAGCAGGC TCTAGAGAAA ATGGGCATTA GTGTACAGGC CAGTGGCATC 
AAGGTAGAGA AGAACAAGTA CTACTTGGTC AATTTAAATG CTGATCCGTC CCTTAACGAG 
CTGCTGGTCT ATTATCTGAA GGATCGCACA CTGATTGGCG GACGCACTAT CAGTGGCCAG 
CAGCCGGATA TACAACTTTC CGGTCTCGGA ATCCAGCCCG AGCACTGTGT GATCACTATC 
GAGGATAGTG GACTGTACAT GGAGCCAGTG CAGGGAGCGC GTTGCTTTGT GAACGGATCT 
GCTGCTGTGG AAAAGACACC ACTCCAGAAC GGCGACCGTA TCCTGTGGGG CAACCACCAC 
TTTTTCCGCG TCAACTCGCC GAAGAGCAAT AACACTAGTA TGTGCGCTTC GGAGCCCCAG 
ACGCCGGCGC AACTGATTGA TTACAATTTC GCACGCGATG AGATTATGCA GAACGAATTG 
AGCAACGATC CTATCCAGAC GGCCATTGCT CGGCTGGAAC GTCAGCACGA GGAAGATAAG 
CAGGTGGCGC TTGAAAAACA ACGGCAGGAG TACGAGCGTC AGTTCCAGCA GCTACGCAAT 
ATTCTGTCGC CTAGTACACC ATATGCACCT TATGCTCCTT ATGATCCACT GCGCATGGGT 
AAGATTACCC CAAATACTCC TACTTCGCAA ATGCGAGTGG AAAAGTGGGC ACAGGAACGA 
GATGAGATGT TTCGTCGCAG TTTGGGTCAG CTGAAAACGG ATATTATGCG TGCGAATTCC 
CTGGTCCAGG AGGCCAACTT TCTGGCTGAG GAGATGGAGA AGAAGACCAA GTTTTCAGTC 
ACTCTGCAGA TTCCGCCAGC TAATCTAAGC CCTAACAGAA GGCGTGGGGC ATTTGTCAGC 
GAACCCGCCA TTCTGGTGAA GCGCACAAAC TCTGGTAGCC AGATCTGGAC GATGGAGAAG 
CTGGAAAACA AGCTGATAGA CATGCGCGAG ATGTACCAGG AGCACAAGGA GCGCGTTCTG 
AACGGACTGC CCCTTATAGA GCCATTCTCA GAAGACGAGT TCGATGACAA GGACGAGGAT 
AATGCCAAGC CACAGGATCC GTTTTACGAG TCGCAAGAGA ACCACAATCT TATTGGCGTG 
GCCAATATAT TCTTGGAGGT TCTGTTTCAT GACGTCAAGC TGGACTACCA CACGCCCATC 
ATTAGCCAGC AAGGCGAGGT AGCGGGTCGG CTTCAGGTTG AGATCGAGCG GATTGCCGGC 
CAGATGCCAC AGGACCGCAT GTGCGAGTCA GTTTCAGAAT CCTCTGGCGA TTCACGGGAT 
GAGTACGACG ACCCTGTGGA TCCCACATCG AATCAGATTA CCTGCCGTGT GACGATCAAG 
TGCGCCAGTG GACTGCCATT GTCGCTCTCC AACTTTGTCT TTTGCCAGTA CACTTTTTGG 
GGTCACCAAG AGATGGTTGT TCCGGTTATC AATGCTGAAT CAACGGCGCA CGACCAGAAC 
ATGGTGTTTA AGTTTGAACA CACCCAGGAC TTCACGGTTA CCATAAACGA AGAGTTTTTG 
GAGCACTGCA TAGAAGGAGC TCTGTCCATT GAGGTGTGGG GCCATCGCAG TGCCGGCTTC 
TCCAAGACAA AGGGCTGGGA AGTCGAACAG CAGCAAGCCA AGGCCCGTTC CCTGGTCGAT 
CGCTGGGCGG AGTTGTCGCG GAAGATCGAG CTTTGGGTGG AGATTCACGA GCTTAATGAC 
AACGGCGAGT ATTCGCCAGT GGAGGTTACC AATCGCAACG AGGTCTTGAC CGGTGGCATT 
TACCAGTTAC GTCAGGGTCA GCAGCGGCGG GTGAATGTAC GGGTGAAGCC GGTGCAAAAC 
TCTGGCACCT TGCCCATTAT CTGCCAGTCG ATTGTGAACG TTGCCATTGG CAGTGTGACA 
GTGCGATCTC GGTTGCAGCG ACCACTGGAT TCTTACCAGG AGGAAGATCT CACCGTGCTG 
CGCGAGAAGT GGAGCGAAGC ACTGGGACGA AGGCGTCAAT ACCTTGACCA ACAGATCCAG 
ATGCTTATAA AGAAAGAGGA GAAGAACGAA CAGGAGCGCG AGAGGGAGCT AAGCTTGGTT 
CATCAGTGGG TCTCGTTGAC GGAGGAACGC AACGCGGTGT TGGTGCCAGC TCCTGGCTCG 
GGCATTCCCG GAGCACCTGC CTCATGGGAG CCGCCGTCCG GAATGGAGCC ACATGTGCCC 
GTCCTCTTCC TCAACCTGAA CGGTGACGAT CTGTCGGCGC AAAACACCAA CGACGAGCTC 
TCCGTCGCCG GCATCAATTC CATTCTCTCC AAGGAGCACG GACATAAGTT TTACACGCTG 
CAAATTCTGC AGCACCTAGA TAAGGATGTT TGCTGTGTGG CCAGCTGGGA CTCTTCGATG 
CATGACAGTC AGGCCCTGAA CCGTGTCACT GAGGCCAATG AGCGGGTCTA TCTCATTCTG 
CGCACAACGG TACGCCTCTC GCACCCAGCA CCTATGGATC TCGTGCTCCG CAAGCGACTA 
AGCATTAACA TCAAGAAGGG ACAGACGCTA ACCGACCGCC TGAAGAAATT CCGTCTTGTG 
CGGGGAGAGA ACGCCATCTG GCAGAGCGGT GTCACCTATG AGGTGGTCTC TAACATTCCA 
AAAGCCTCCG AGGAGTTGGA GGACCGCGAG TCTCTTGCAC AGTTGGCTGC CAGCGGAGAT 
GATTGCTCCG CAAGTGACGG CGAAACCTAC ATAGAGAAAT ACACGCGCGG TGTTTCGGCG 
GTGGAGAGCA TACTGACTCT GGATAGACTG CGGCAGAATG TGGCGGTCAA GGAGCTGGAA 
ACGGCACATG GACAGCCGCT CAGCATGCGC AAGACCGTCA GCGTGCCGAA CTTCTCACAG 
ATTATGCGCT TCGATGCATC GATGGAGTCG CTGCTGAATG TGGGACGATC CGAGTCCTTT 
GCCGATCTCA ATAACAGCGC TTTGGGCAAC AAGTTTACTC CAGCAGGTCA TAGTCCAGCA 
GGAGCAGGCG GAGTCATCCG AAGTCGCCAC AGCTTTGGAG GCAAAGGAAG CAGCGATGAT 
TCTCCCGGAA AAGCCTTTGG CATTGCTTCG CCAGCTACTA GTAAGCTGCT GGGCATGCGC 
ATGACTACGT TGCACGAGGA GCCACTGGGT GGACACAGAT CTCTCGACGA AGAGCCTGAG 
GACAGCTACA GCGACTCGGA ATATGCTGCC GAGTACGAAC AGGAGCGGCA GCAGAACAAA 
AGCATGGCCA CGCGATCCCG CCTCACGGCT TCCAAGACCA TGGACTCATT CATGGACGTC 
AGCAGCCATT CGAACCAAAG CTACTTGAGC TACACGTCCA GTGCCAACGC TAACATGAAG 
CACCTGACCG GCCTGGCGAC TTTGAGCATG AGCTCATCCA CCAGCAGTGG CTACGGTTCC 
CAGGCTGTCT CCTGCAATAA TCTGAGCAAC GAGGATATTG CTTCAATGCG TTCCATGAGC 
ATTGATGAGA CACCAGATTT CGATCGCGTT AACTCGAATT CGCCACCAAA CCGGCAGGCA 
CGAGTTAACC CCTTTCTCAA GGACATGCCC AAAGCTAAAA TACAAGAGCA ACCGGAACAG 
CAGGCCAAGA AGCTGCAGGA AGCATTTACG CATCCGTTGG AGCAGCTGGA GTCCAGTCAA 
AACGCACAAA GCGACGATGA TGAATGCGCG CAACTGCCAA AGAATAATAA CAACAACTTG 
GACTTGGTAA ACGAGCCGAA GCCACTTTCA AGCCAAACGG ATCTGGAGGA ATCTATGTCG 
CAACCTAAAT CAAAAACGGA GTTTGCCACA GACAATCAGA ACGGCAACAG GTCCTCTGAC 
GAGTTAAGCC ACAGTTCCGA GGATCTGCTC GAAGGCGATG GCATCGTCCG GGAAGAGTTG 
CCCGCTGGAA AGGTGGTGCG GCGAAAGAAG TCCAATACCC AGCCCCCGAG CAATGGCAAC 
AGCATAAATA ACAATAACAA CGGCACAACC CAGGCACCGC GCATCAATCA TCGGGCATCG 
GTGGCCAAAA TGGAGGGTCT GGCTGCATAT TTGGATTCTA GCATTATGAC CAGCAGCACC 
GAAGTTGATG AGGAGAGCAA GGATGTGGAA TTGGTTCTGC CCGAGTGGAT AGTGGTGGGC 
GAGTCAGTGT TAATCCGGCC GTATAACACC AGCGGCGTCA TTCGCTTTGT GGGAACCACG 
GAATTCCAAC CCGGTGCCTG GATTGGCGTT GAATTGGACA CACCAACGGG CAAAAACGAC 
GGCAGCGTGA AGGGCGTTCA GTATTTCCAG TGCAAGCCCA AGCACGGCAT GTTTGTGCGC 
TCCGACAAGC TGATGCTGGA CAAGCGTGGC AAGGCGATGC GAGCCTACAA GGCCGCCGAG 
AAGAGCAACA GCATCAGCAA AGAGATGAGT ACCTCGATGA CTGGCTCGAT GACACGCTCC 
AAGAGCCGCG GCGATTCGCT AAACCTTTCG GCGCGTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Khc-73 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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