Lap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lap1
Entrez Gene ID	36670
Full Name	CG10255 gene product from transcript CG10255-RB
Synonyms	CG10255,CT28815,Dmel\CG10255,dLAP1,dlap1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51D4-51D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_137163.5	NP_611007.1	Lap1, isoform A
NM_001202009.2	NP_001188938.1	Lap1, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10044	NM_001202009.2 Latest version!	Drosophila melanogaster Lap1, transcript variant B (Lap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa10044	NM_137163.5 Latest version!	Drosophila melanogaster Lap1, transcript variant A (Lap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lap1 ( NM_137163.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611007.1 Drosophila melanogaster Lap1, transcript variant A (Lap1), mRNA.

Price & Availability↑

CloneID	OFa10044
Clone ID Related Accession (Same CDS sequence)	NM_137163.5 , NM_001202009.2
Accession Version	NM_137163.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lap1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137163.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCTGC TGAGCAAATG CTTCCCCTGC TTCAAATTCA AGCGCGAGGA GGTGATCGAC 
AAGCTGGACT ACAGTAATAC CCCGCTAACG GATTTCCCAG AAGTTTGGCA GCACGAGCGA 
ACCCTGGAGG AGCTTTACCT GAGCACCACA AGATTGCAAG CTCTGCCACC GCAATTGTTT 
TATTGCCAAG GATTAAGGGT GCTTCACGTA AACAGCAACA ATTTGGAGAG CATTCCACAG 
GCCATTGGCA GTCTGCGACA GCTGCAGCAT CTGGATCTCA ACCGGAATCT TATTGTCAAT 
GTGCCCGAGG AAATCAAGTC CTGTAAGCAC TTAACCCACT TGGACCTGAG CTGCAACAGT 
TTGCAACGTC TTCCCGATGC CATTACCTCG CTAATTTCGC TGCAGGAGCT GCTGCTAAAC 
GAGACCTATC TAGAATTCCT ACCAGCCAAC TTTGGCCGAT TGGTAAATCT GAGAATTCTG 
GAGCTGCGAC TCAACAATCT CATGACACTT CCCAAATCCA TGGTGAGACT AATCAACCTA 
CAGAGGCTGG ACATTGGCGG TAATGAATTT ACCGAGTTGC CCGAAGTTGT CGGCGAGTTG 
AAGTCCCTTC GCGAACTATG GATCGACTTC AATCAAATAC GTCGTGTTTC GGCCAACATT 
GGAAAGCTAC GCGATCTGCA GCACTTCGAG GCCAATGGCA ACTTGCTGGA CACTCTTCCC 
AGCGAGCTGA GCAACTGGCG CAATGTGGAG GTACTATCTA TATGCTCCAA CAGCCTGGAG 
GCCTTTCCCT TCAGCGTGGG CATGCTGAAG TCCCTGGTGA CATTCAAGTG CGAGTCAAAC 
GGTTTGACAG AGTTACCCGA CAGCATAAGC TACTTGGAAC AGCTCGAGGA ACTAGTATTA 
AGCCACAACA AGCTTATACG CCTACCTAGC ACAATTGGGA TGTTGCGTAG CCTTCGCTTT 
CTGTTCGCCG ATGATAATCA ACTACGACAA CTACCAGATG AGCTGTGCAG CTGTCAGCAG 
TTGAGTGTGC TAAGTGTGGC GAACAATCAG TTGTCCGCTC TACCACAGAA CATTGGAAAC 
CTGAGCAAGA TGAAGGTACT CAATGTGGTG AATAACTACA TAAATGCATT GCCAGTTTCG 
ATGTTAAATC TGGTTAATCT CACATCGATG TGGCTAAGCG ATAACCAATC GCAGCCCTTG 
GTGCCGCTGC AATATCTAGA TGCAAGTACA AAGACCCAGT TGACCTGCTT CATGCTGCCC 
CAAGTCACAT TCAAGATGAA CAGTATACAG GCCCAACAGC AGGCTCAGGA GCAGTATGAG 
TTCGTTTACG CCAACCAGCA GCAGCCTCAC GCGAGTCCCT CGAGAAGGAT TTGCTTCGCC 
GAGGAGGCCA CCATTCTTAG CAACGCCAAA GCACAGCCAG CGCCCAATTA TCCCAGCTTT 
GTGGCCGCTC CGCCGACGCC AACGCCCGAT CAGATGGCTG GGTCTGTGCG GTTAATGCGT 
TCACCCACAC CTTATCCCAA GGAGCTGCGA CAAATGTCCA AATATGTGAG GCAGGCTCAG 
GCGGCGACCT CATCGGCCAA TGCCAGCGAG GTAAGGGAGG CACGCGTAGT AACCAATGGA 
CAAATTCACT GTGATAGCAA CAATGCCAAC CAGGATGTTG TGGACCAGGC CACAACAAGT 
GCAATATACG GCATTGCGCC CGAAACGACA CACATCTACG GAGTCTATCA GCAACCGCAG 
CAGATGGCGC ACCCGGTGCC AACGCAGGAG TACTACGGCC TGCCACTGGT CAACTACGAA 
GCACACTATC AGCAGCTTTA CGTCGAGGCC AACACGCCGC TTCCCACCAC GCATTTAAAC 
GGGGATCAGG ACTATGAGTT GCAACCGCTG CAGCAACAAC CAATGCAGCA ACAGGCGCTG 
CCAACACCCC GGTTGGAACC ACCACCATAC CACATAGCTC GAGTTTACAC AAAGAAAACG 
CCCGAGGATC TCAACCTTTA CGAGTCCATG AGGCAGCGAA AGCAGCAACA ACAGCTGCAA 
GAACAAACCA TCTACCAAGA TGCTTTGAAT AGCAATAGTA ACTTTAAAAC GACAGCGATT 
GGCGCTCAGG ACGTCGAAGA GTCAGTCGAT CAGTTGGACT ACCAAAATAA TATTAGCAAT 
AATTTAGAGC CAAATCCGGA GGAGGAAGAC CAGGAGCTGG ATGACACTAT GTCGCAGCAC 
TCGCTTAATT CCACGGCCAC TAACAATACT TCCAAAGCGA GCCATAAGAA ATCCACCTGG 
ATCTTTGGTG TCCACAAAAA TCCGACAGTC AAACAGGTTA CTCTAAAATG GGAGAACTCT 
ATAGGCTTTG ATATAGCCGA GCTTCTTAAT CAGGTTGGCA TCTTTGTGAG CTCCATAACG 
CCCAATACGA ATGCCGCCCG CCTGCTTAAT CTGAACGACA AGTTGCTGGA AATCGACGGC 
TACGACCTGA CCAATGCCAA CCTGAGCGAT GCCAAACGAG TGCTGCTAAA CTGCGGCACG 
GTCATGAACA TAATGTTGTC GAGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611007.1
CDS	397..2946
Translation	MPLLSKCFPCFKFKREEVIDKLDYSNTPLTDFPEVWQHERTLEELYLSTTRLQALPPQLFYCQGLRVLHVNSNNLESIPQAIGSLRQLQHLDLNRNLIVNVPEEIKSCKHLTHLDLSCNSLQRLPDAITSLISLQELLLNETYLEFLPANFGRLVNLRILELRLNNLMTLPKSMVRLINLQRLDIGGNEFTELPEVVGELKSLRELWIDFNQIRRVSANIGKLRDLQHFEANGNLLDTLPSELSNWRNVEVLSICSNSLEAFPFSVGMLKSLVTFKCESNGLTELPDSISYLEQLEELVLSHNKLIRLPSTIGMLRSLRFLFADDNQLRQLPDELCSCQQLSVLSVANNQLSALPQNIGNLSKMKVLNVVNNYINALPVSMLNLVNLTSMWLSDNQSQPLVPLQYLDASTKTQLTCFMLPQVTFKMNSIQAQQQAQEQYEFVYANQQQPHASPSRRICFAEEATILSNAKAQPAPNYPSFVAAPPTPTPDQMAGSVRLMRSPTPYPKELRQMSKYVRQAQAATSSANASEVREARVVTNGQIHCDSNNANQDVVDQATTSAIYGIAPETTHIYGVYQQPQQMAHPVPTQEYYGLPLVNYEAHYQQLYVEANTPLPTTHLNGDQDYELQPLQQQPMQQQALPTPRLEPPPYHIARVYTKKTPEDLNLYESMRQRKQQQQLQEQTIYQDALNSNSNFKTTAIGAQDVEESVDQLDYQNNISNNLEPNPEEEDQELDDTMSQHSLNSTATNNTSKASHKKSTWIFGVHKNPTVKQVTLKWENSIGFDIAELLNQVGIFVSSITPNTNAARLLNLNDKLLEIDGYDLTNANLSDAKRVLLNCGTVMNIMLSRK

Target ORF information:

RefSeq Version	NM_137163.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lap1, transcript variant A (Lap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137163.5

ORF Insert Sequence:

ATGCCGCTGC TGAGCAAATG CTTCCCCTGC TTCAAATTCA AGCGCGAGGA GGTGATCGAC 
AAGCTGGACT ACAGTAATAC CCCGCTAACG GATTTCCCAG AAGTTTGGCA GCACGAGCGA 
ACCCTGGAGG AGCTTTACCT GAGCACCACA AGATTGCAAG CTCTGCCACC GCAATTGTTT 
TATTGCCAAG GATTAAGGGT GCTTCACGTA AACAGCAACA ATTTGGAGAG CATTCCACAG 
GCCATTGGCA GTCTGCGACA GCTGCAGCAT CTGGATCTCA ACCGGAATCT TATTGTCAAT 
GTGCCCGAGG AAATCAAGTC CTGTAAGCAC TTAACCCACT TGGACCTGAG CTGCAACAGT 
TTGCAACGTC TTCCCGATGC CATTACCTCG CTAATTTCGC TGCAGGAGCT GCTGCTAAAC 
GAGACCTATC TAGAATTCCT ACCAGCCAAC TTTGGCCGAT TGGTAAATCT GAGAATTCTG 
GAGCTGCGAC TCAACAATCT CATGACACTT CCCAAATCCA TGGTGAGACT AATCAACCTA 
CAGAGGCTGG ACATTGGCGG TAATGAATTT ACCGAGTTGC CCGAAGTTGT CGGCGAGTTG 
AAGTCCCTTC GCGAACTATG GATCGACTTC AATCAAATAC GTCGTGTTTC GGCCAACATT 
GGAAAGCTAC GCGATCTGCA GCACTTCGAG GCCAATGGCA ACTTGCTGGA CACTCTTCCC 
AGCGAGCTGA GCAACTGGCG CAATGTGGAG GTACTATCTA TATGCTCCAA CAGCCTGGAG 
GCCTTTCCCT TCAGCGTGGG CATGCTGAAG TCCCTGGTGA CATTCAAGTG CGAGTCAAAC 
GGTTTGACAG AGTTACCCGA CAGCATAAGC TACTTGGAAC AGCTCGAGGA ACTAGTATTA 
AGCCACAACA AGCTTATACG CCTACCTAGC ACAATTGGGA TGTTGCGTAG CCTTCGCTTT 
CTGTTCGCCG ATGATAATCA ACTACGACAA CTACCAGATG AGCTGTGCAG CTGTCAGCAG 
TTGAGTGTGC TAAGTGTGGC GAACAATCAG TTGTCCGCTC TACCACAGAA CATTGGAAAC 
CTGAGCAAGA TGAAGGTACT CAATGTGGTG AATAACTACA TAAATGCATT GCCAGTTTCG 
ATGTTAAATC TGGTTAATCT CACATCGATG TGGCTAAGCG ATAACCAATC GCAGCCCTTG 
GTGCCGCTGC AATATCTAGA TGCAAGTACA AAGACCCAGT TGACCTGCTT CATGCTGCCC 
CAAGTCACAT TCAAGATGAA CAGTATACAG GCCCAACAGC AGGCTCAGGA GCAGTATGAG 
TTCGTTTACG CCAACCAGCA GCAGCCTCAC GCGAGTCCCT CGAGAAGGAT TTGCTTCGCC 
GAGGAGGCCA CCATTCTTAG CAACGCCAAA GCACAGCCAG CGCCCAATTA TCCCAGCTTT 
GTGGCCGCTC CGCCGACGCC AACGCCCGAT CAGATGGCTG GGTCTGTGCG GTTAATGCGT 
TCACCCACAC CTTATCCCAA GGAGCTGCGA CAAATGTCCA AATATGTGAG GCAGGCTCAG 
GCGGCGACCT CATCGGCCAA TGCCAGCGAG GTAAGGGAGG CACGCGTAGT AACCAATGGA 
CAAATTCACT GTGATAGCAA CAATGCCAAC CAGGATGTTG TGGACCAGGC CACAACAAGT 
GCAATATACG GCATTGCGCC CGAAACGACA CACATCTACG GAGTCTATCA GCAACCGCAG 
CAGATGGCGC ACCCGGTGCC AACGCAGGAG TACTACGGCC TGCCACTGGT CAACTACGAA 
GCACACTATC AGCAGCTTTA CGTCGAGGCC AACACGCCGC TTCCCACCAC GCATTTAAAC 
GGGGATCAGG ACTATGAGTT GCAACCGCTG CAGCAACAAC CAATGCAGCA ACAGGCGCTG 
CCAACACCCC GGTTGGAACC ACCACCATAC CACATAGCTC GAGTTTACAC AAAGAAAACG 
CCCGAGGATC TCAACCTTTA CGAGTCCATG AGGCAGCGAA AGCAGCAACA ACAGCTGCAA 
GAACAAACCA TCTACCAAGA TGCTTTGAAT AGCAATAGTA ACTTTAAAAC GACAGCGATT 
GGCGCTCAGG ACGTCGAAGA GTCAGTCGAT CAGTTGGACT ACCAAAATAA TATTAGCAAT 
AATTTAGAGC CAAATCCGGA GGAGGAAGAC CAGGAGCTGG ATGACACTAT GTCGCAGCAC 
TCGCTTAATT CCACGGCCAC TAACAATACT TCCAAAGCGA GCCATAAGAA ATCCACCTGG 
ATCTTTGGTG TCCACAAAAA TCCGACAGTC AAACAGGTTA CTCTAAAATG GGAGAACTCT 
ATAGGCTTTG ATATAGCCGA GCTTCTTAAT CAGGTTGGCA TCTTTGTGAG CTCCATAACG 
CCCAATACGA ATGCCGCCCG CCTGCTTAAT CTGAACGACA AGTTGCTGGA AATCGACGGC 
TACGACCTGA CCAATGCCAA CCTGAGCGAT GCCAAACGAG TGCTGCTAAA CTGCGGCACG 
GTCATGAACA TAATGTTGTC GAGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lap1 ( NM_001202009.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188938.1 Drosophila melanogaster Lap1, transcript variant B (Lap1), mRNA.

Price & Availability↑

CloneID	OFa10044
Clone ID Related Accession (Same CDS sequence)	NM_137163.5 , NM_001202009.2
Accession Version	NM_001202009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lap1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001202009.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCTGC TGAGCAAATG CTTCCCCTGC TTCAAATTCA AGCGCGAGGA GGTGATCGAC 
AAGCTGGACT ACAGTAATAC CCCGCTAACG GATTTCCCAG AAGTTTGGCA GCACGAGCGA 
ACCCTGGAGG AGCTTTACCT GAGCACCACA AGATTGCAAG CTCTGCCACC GCAATTGTTT 
TATTGCCAAG GATTAAGGGT GCTTCACGTA AACAGCAACA ATTTGGAGAG CATTCCACAG 
GCCATTGGCA GTCTGCGACA GCTGCAGCAT CTGGATCTCA ACCGGAATCT TATTGTCAAT 
GTGCCCGAGG AAATCAAGTC CTGTAAGCAC TTAACCCACT TGGACCTGAG CTGCAACAGT 
TTGCAACGTC TTCCCGATGC CATTACCTCG CTAATTTCGC TGCAGGAGCT GCTGCTAAAC 
GAGACCTATC TAGAATTCCT ACCAGCCAAC TTTGGCCGAT TGGTAAATCT GAGAATTCTG 
GAGCTGCGAC TCAACAATCT CATGACACTT CCCAAATCCA TGGTGAGACT AATCAACCTA 
CAGAGGCTGG ACATTGGCGG TAATGAATTT ACCGAGTTGC CCGAAGTTGT CGGCGAGTTG 
AAGTCCCTTC GCGAACTATG GATCGACTTC AATCAAATAC GTCGTGTTTC GGCCAACATT 
GGAAAGCTAC GCGATCTGCA GCACTTCGAG GCCAATGGCA ACTTGCTGGA CACTCTTCCC 
AGCGAGCTGA GCAACTGGCG CAATGTGGAG GTACTATCTA TATGCTCCAA CAGCCTGGAG 
GCCTTTCCCT TCAGCGTGGG CATGCTGAAG TCCCTGGTGA CATTCAAGTG CGAGTCAAAC 
GGTTTGACAG AGTTACCCGA CAGCATAAGC TACTTGGAAC AGCTCGAGGA ACTAGTATTA 
AGCCACAACA AGCTTATACG CCTACCTAGC ACAATTGGGA TGTTGCGTAG CCTTCGCTTT 
CTGTTCGCCG ATGATAATCA ACTACGACAA CTACCAGATG AGCTGTGCAG CTGTCAGCAG 
TTGAGTGTGC TAAGTGTGGC GAACAATCAG TTGTCCGCTC TACCACAGAA CATTGGAAAC 
CTGAGCAAGA TGAAGGTACT CAATGTGGTG AATAACTACA TAAATGCATT GCCAGTTTCG 
ATGTTAAATC TGGTTAATCT CACATCGATG TGGCTAAGCG ATAACCAATC GCAGCCCTTG 
GTGCCGCTGC AATATCTAGA TGCAAGTACA AAGACCCAGT TGACCTGCTT CATGCTGCCC 
CAAGTCACAT TCAAGATGAA CAGTATACAG GCCCAACAGC AGGCTCAGGA GCAGTATGAG 
TTCGTTTACG CCAACCAGCA GCAGCCTCAC GCGAGTCCCT CGAGAAGGAT TTGCTTCGCC 
GAGGAGGCCA CCATTCTTAG CAACGCCAAA GCACAGCCAG CGCCCAATTA TCCCAGCTTT 
GTGGCCGCTC CGCCGACGCC AACGCCCGAT CAGATGGCTG GGTCTGTGCG GTTAATGCGT 
TCACCCACAC CTTATCCCAA GGAGCTGCGA CAAATGTCCA AATATGTGAG GCAGGCTCAG 
GCGGCGACCT CATCGGCCAA TGCCAGCGAG GTAAGGGAGG CACGCGTAGT AACCAATGGA 
CAAATTCACT GTGATAGCAA CAATGCCAAC CAGGATGTTG TGGACCAGGC CACAACAAGT 
GCAATATACG GCATTGCGCC CGAAACGACA CACATCTACG GAGTCTATCA GCAACCGCAG 
CAGATGGCGC ACCCGGTGCC AACGCAGGAG TACTACGGCC TGCCACTGGT CAACTACGAA 
GCACACTATC AGCAGCTTTA CGTCGAGGCC AACACGCCGC TTCCCACCAC GCATTTAAAC 
GGGGATCAGG ACTATGAGTT GCAACCGCTG CAGCAACAAC CAATGCAGCA ACAGGCGCTG 
CCAACACCCC GGTTGGAACC ACCACCATAC CACATAGCTC GAGTTTACAC AAAGAAAACG 
CCCGAGGATC TCAACCTTTA CGAGTCCATG AGGCAGCGAA AGCAGCAACA ACAGCTGCAA 
GAACAAACCA TCTACCAAGA TGCTTTGAAT AGCAATAGTA ACTTTAAAAC GACAGCGATT 
GGCGCTCAGG ACGTCGAAGA GTCAGTCGAT CAGTTGGACT ACCAAAATAA TATTAGCAAT 
AATTTAGAGC CAAATCCGGA GGAGGAAGAC CAGGAGCTGG ATGACACTAT GTCGCAGCAC 
TCGCTTAATT CCACGGCCAC TAACAATACT TCCAAAGCGA GCCATAAGAA ATCCACCTGG 
ATCTTTGGTG TCCACAAAAA TCCGACAGTC AAACAGGTTA CTCTAAAATG GGAGAACTCT 
ATAGGCTTTG ATATAGCCGA GCTTCTTAAT CAGGTTGGCA TCTTTGTGAG CTCCATAACG 
CCCAATACGA ATGCCGCCCG CCTGCTTAAT CTGAACGACA AGTTGCTGGA AATCGACGGC 
TACGACCTGA CCAATGCCAA CCTGAGCGAT GCCAAACGAG TGCTGCTAAA CTGCGGCACG 
GTCATGAACA TAATGTTGTC GAGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188938.1
CDS	414..2963
Translation	MPLLSKCFPCFKFKREEVIDKLDYSNTPLTDFPEVWQHERTLEELYLSTTRLQALPPQLFYCQGLRVLHVNSNNLESIPQAIGSLRQLQHLDLNRNLIVNVPEEIKSCKHLTHLDLSCNSLQRLPDAITSLISLQELLLNETYLEFLPANFGRLVNLRILELRLNNLMTLPKSMVRLINLQRLDIGGNEFTELPEVVGELKSLRELWIDFNQIRRVSANIGKLRDLQHFEANGNLLDTLPSELSNWRNVEVLSICSNSLEAFPFSVGMLKSLVTFKCESNGLTELPDSISYLEQLEELVLSHNKLIRLPSTIGMLRSLRFLFADDNQLRQLPDELCSCQQLSVLSVANNQLSALPQNIGNLSKMKVLNVVNNYINALPVSMLNLVNLTSMWLSDNQSQPLVPLQYLDASTKTQLTCFMLPQVTFKMNSIQAQQQAQEQYEFVYANQQQPHASPSRRICFAEEATILSNAKAQPAPNYPSFVAAPPTPTPDQMAGSVRLMRSPTPYPKELRQMSKYVRQAQAATSSANASEVREARVVTNGQIHCDSNNANQDVVDQATTSAIYGIAPETTHIYGVYQQPQQMAHPVPTQEYYGLPLVNYEAHYQQLYVEANTPLPTTHLNGDQDYELQPLQQQPMQQQALPTPRLEPPPYHIARVYTKKTPEDLNLYESMRQRKQQQQLQEQTIYQDALNSNSNFKTTAIGAQDVEESVDQLDYQNNISNNLEPNPEEEDQELDDTMSQHSLNSTATNNTSKASHKKSTWIFGVHKNPTVKQVTLKWENSIGFDIAELLNQVGIFVSSITPNTNAARLLNLNDKLLEIDGYDLTNANLSDAKRVLLNCGTVMNIMLSRK

Target ORF information:

RefSeq Version	NM_001202009.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lap1, transcript variant B (Lap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202009.2

ORF Insert Sequence:

ATGCCGCTGC TGAGCAAATG CTTCCCCTGC TTCAAATTCA AGCGCGAGGA GGTGATCGAC 
AAGCTGGACT ACAGTAATAC CCCGCTAACG GATTTCCCAG AAGTTTGGCA GCACGAGCGA 
ACCCTGGAGG AGCTTTACCT GAGCACCACA AGATTGCAAG CTCTGCCACC GCAATTGTTT 
TATTGCCAAG GATTAAGGGT GCTTCACGTA AACAGCAACA ATTTGGAGAG CATTCCACAG 
GCCATTGGCA GTCTGCGACA GCTGCAGCAT CTGGATCTCA ACCGGAATCT TATTGTCAAT 
GTGCCCGAGG AAATCAAGTC CTGTAAGCAC TTAACCCACT TGGACCTGAG CTGCAACAGT 
TTGCAACGTC TTCCCGATGC CATTACCTCG CTAATTTCGC TGCAGGAGCT GCTGCTAAAC 
GAGACCTATC TAGAATTCCT ACCAGCCAAC TTTGGCCGAT TGGTAAATCT GAGAATTCTG 
GAGCTGCGAC TCAACAATCT CATGACACTT CCCAAATCCA TGGTGAGACT AATCAACCTA 
CAGAGGCTGG ACATTGGCGG TAATGAATTT ACCGAGTTGC CCGAAGTTGT CGGCGAGTTG 
AAGTCCCTTC GCGAACTATG GATCGACTTC AATCAAATAC GTCGTGTTTC GGCCAACATT 
GGAAAGCTAC GCGATCTGCA GCACTTCGAG GCCAATGGCA ACTTGCTGGA CACTCTTCCC 
AGCGAGCTGA GCAACTGGCG CAATGTGGAG GTACTATCTA TATGCTCCAA CAGCCTGGAG 
GCCTTTCCCT TCAGCGTGGG CATGCTGAAG TCCCTGGTGA CATTCAAGTG CGAGTCAAAC 
GGTTTGACAG AGTTACCCGA CAGCATAAGC TACTTGGAAC AGCTCGAGGA ACTAGTATTA 
AGCCACAACA AGCTTATACG CCTACCTAGC ACAATTGGGA TGTTGCGTAG CCTTCGCTTT 
CTGTTCGCCG ATGATAATCA ACTACGACAA CTACCAGATG AGCTGTGCAG CTGTCAGCAG 
TTGAGTGTGC TAAGTGTGGC GAACAATCAG TTGTCCGCTC TACCACAGAA CATTGGAAAC 
CTGAGCAAGA TGAAGGTACT CAATGTGGTG AATAACTACA TAAATGCATT GCCAGTTTCG 
ATGTTAAATC TGGTTAATCT CACATCGATG TGGCTAAGCG ATAACCAATC GCAGCCCTTG 
GTGCCGCTGC AATATCTAGA TGCAAGTACA AAGACCCAGT TGACCTGCTT CATGCTGCCC 
CAAGTCACAT TCAAGATGAA CAGTATACAG GCCCAACAGC AGGCTCAGGA GCAGTATGAG 
TTCGTTTACG CCAACCAGCA GCAGCCTCAC GCGAGTCCCT CGAGAAGGAT TTGCTTCGCC 
GAGGAGGCCA CCATTCTTAG CAACGCCAAA GCACAGCCAG CGCCCAATTA TCCCAGCTTT 
GTGGCCGCTC CGCCGACGCC AACGCCCGAT CAGATGGCTG GGTCTGTGCG GTTAATGCGT 
TCACCCACAC CTTATCCCAA GGAGCTGCGA CAAATGTCCA AATATGTGAG GCAGGCTCAG 
GCGGCGACCT CATCGGCCAA TGCCAGCGAG GTAAGGGAGG CACGCGTAGT AACCAATGGA 
CAAATTCACT GTGATAGCAA CAATGCCAAC CAGGATGTTG TGGACCAGGC CACAACAAGT 
GCAATATACG GCATTGCGCC CGAAACGACA CACATCTACG GAGTCTATCA GCAACCGCAG 
CAGATGGCGC ACCCGGTGCC AACGCAGGAG TACTACGGCC TGCCACTGGT CAACTACGAA 
GCACACTATC AGCAGCTTTA CGTCGAGGCC AACACGCCGC TTCCCACCAC GCATTTAAAC 
GGGGATCAGG ACTATGAGTT GCAACCGCTG CAGCAACAAC CAATGCAGCA ACAGGCGCTG 
CCAACACCCC GGTTGGAACC ACCACCATAC CACATAGCTC GAGTTTACAC AAAGAAAACG 
CCCGAGGATC TCAACCTTTA CGAGTCCATG AGGCAGCGAA AGCAGCAACA ACAGCTGCAA 
GAACAAACCA TCTACCAAGA TGCTTTGAAT AGCAATAGTA ACTTTAAAAC GACAGCGATT 
GGCGCTCAGG ACGTCGAAGA GTCAGTCGAT CAGTTGGACT ACCAAAATAA TATTAGCAAT 
AATTTAGAGC CAAATCCGGA GGAGGAAGAC CAGGAGCTGG ATGACACTAT GTCGCAGCAC 
TCGCTTAATT CCACGGCCAC TAACAATACT TCCAAAGCGA GCCATAAGAA ATCCACCTGG 
ATCTTTGGTG TCCACAAAAA TCCGACAGTC AAACAGGTTA CTCTAAAATG GGAGAACTCT 
ATAGGCTTTG ATATAGCCGA GCTTCTTAAT CAGGTTGGCA TCTTTGTGAG CTCCATAACG 
CCCAATACGA ATGCCGCCCG CCTGCTTAAT CTGAACGACA AGTTGCTGGA AATCGACGGC 
TACGACCTGA CCAATGCCAA CCTGAGCGAT GCCAAACGAG TGCTGCTAAA CTGCGGCACG 
GTCATGAACA TAATGTTGTC GAGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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