CG12857 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12857
Entrez Gene ID	36642
Full Name	CG12857 gene product from transcript CG12857-RB
Synonyms	Dmel\CG12857
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51C2-51C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274048.1	NP_001260977.1	uncharacterized protein, isoform B
NM_137143.2	NP_610987.1	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG9970	NP_647754.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009292	XP_001238250.1
Drosophila melanogaster (fruit fly)	CG12692	NP_572159.1
Drosophila melanogaster (fruit fly)	CG30357	NP_724707.1
Drosophila melanogaster (fruit fly)	CG12857	NP_610987.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12857 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12857 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10017	NM_137143.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12857), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12548	NM_001274048.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12857), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12857 ( NM_137143.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610987.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12857), mRNA.

Price & Availability↑

CloneID	OFa10017
Clone ID Related Accession (Same CDS sequence)	NM_137143.2
Accession Version	NM_137143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137143.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGC TAATGAGGCA GAACGGACAG AAATGCGAGA GGCAGGAACT CACCTGCAGC 
GTTTTATTTA CCAATCCCAA GCTTAGTGCC GAGAAGTTAT CCTCCAATGA TGAAATAAAC 
TCCATTGCGA GATTCGTATC GAAGGCGGAG GATCTCGAAA TGCATAGAGT GTTCAGTAAG 
ATATCAAAGA ATGGTGTCGA GGAGACCACG TCTCTGAATC GCTCCCTCTT CGAGTTCGGT 
CATAATTTCG AGGATGCCGA TGGCTGGCTG AAACTGGAGC AGCCTTGCAA GGATCGGCTG 
GCCACGGTGC TGCGCTACAT GTTCGAGTCC CATCTGAAGA CCACTGTGCA GATAGAAATC 
AACAATATGT ACTTCAATTG CCACTTCATC GTGCTGCAGG TGTACTCTCG CTTCTTTAGT 
GAACTGGAGA TGATTCCGCT GCTGGTCACC CTGCCCGAGA AGATCGTGTC CCAGAAGGCC 
TTTATGCTCT CCTACAAATG GATGCTGAGC GATGAGCCCG TCCTGGAGCT GGCTCACATT 
GTGGAGGTCT ATGTGGCCGC CACTTATTTG AGGATCTCTG GTCTGGCGGC GCATTGTTGG 
AAATATTTCG ATGATGAGGA ATACTACAAC GAGGATACGG CATGTGTCCT ATACGTCGAG 
TCGAAGGACA ATCCCGCCAT GGATGTAGTT CGCAATTTGA TGCTGACTAG GATCCGCAAG 
TTTCTGCTCA CCTTTGTGGC CACCAGGGAT TTCCTCGATC TGCCGACCTC CCATCTGATT 
TTTTTGCTCG AATCCGATCA AATCTGTGTG AACACCGAGA TTGAGGTTTT CTTTATAGCT 
GTTCGATGGC TGGGTCACGA TTGGAACAAG AGGAAAGTGC ACGTACGTCG TTTAATGTCC 
TGTATTCGAT TCAACCTGAT GCCATTGTGG TATCTACTAT ATGCGCGACG GGAGGAGGAT 
CACCCGTTGG TGATGAAGCT GATTTTCAAC CCGGAGGTGG AGTACAAGAT CGACGAATCC 
ATATCGAGAA TAACGTCGCG CATGTACGAG GATGCCTTGG AGGGCAATGA CGCACAGAGT 
GAGGAATACG CAAGTGATTC CGGACAGCGA AACTGGATAT GCGATAGCTT GTGCAGCTAT 
TATCACGGCG TCGGTTGTCC CAATACCCGG GAAATTCGTT TCAAACACTT CGAGGATTAC 
CTTTCGGAAT TACAACAATG CTCCAAAGAT CACTGGTCTC AGGTGATATT TCAGGACCCG 
AGTAAAAAGA TTGACTGTTG CAGCACAAGG AAGATAAAAT CGGACGATTT TGTTGCCGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610987.1
CDS	47..1369
Translation	MEELMRQNGQKCERQELTCSVLFTNPKLSAEKLSSNDEINSIARFVSKAEDLEMHRVFSKISKNGVEETTSLNRSLFEFGHNFEDADGWLKLEQPCKDRLATVLRYMFESHLKTTVQIEINNMYFNCHFIVLQVYSRFFSELEMIPLLVTLPEKIVSQKAFMLSYKWMLSDEPVLELAHIVEVYVAATYLRISGLAAHCWKYFDDEEYYNEDTACVLYVESKDNPAMDVVRNLMLTRIRKFLLTFVATRDFLDLPTSHLIFLLESDQICVNTEIEVFFIAVRWLGHDWNKRKVHVRRLMSCIRFNLMPLWYLLYARREEDHPLVMKLIFNPEVEYKIDESISRITSRMYEDALEGNDAQSEEYASDSGQRNWICDSLCSYYHGVGCPNTREIRFKHFEDYLSELQQCSKDHWSQVIFQDPSKKIDCCSTRKIKSDDFVAD

Target ORF information:

RefSeq Version	NM_137143.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12857), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137143.2

ORF Insert Sequence:

ATGGAGGAGC TAATGAGGCA GAACGGACAG AAATGCGAGA GGCAGGAACT CACCTGCAGC 
GTTTTATTTA CCAATCCCAA GCTTAGTGCC GAGAAGTTAT CCTCCAATGA TGAAATAAAC 
TCCATTGCGA GATTCGTATC GAAGGCGGAG GATCTCGAAA TGCATAGAGT GTTCAGTAAG 
ATATCAAAGA ATGGTGTCGA GGAGACCACG TCTCTGAATC GCTCCCTCTT CGAGTTCGGT 
CATAATTTCG AGGATGCCGA TGGCTGGCTG AAACTGGAGC AGCCTTGCAA GGATCGGCTG 
GCCACGGTGC TGCGCTACAT GTTCGAGTCC CATCTGAAGA CCACTGTGCA GATAGAAATC 
AACAATATGT ACTTCAATTG CCACTTCATC GTGCTGCAGG TGTACTCTCG CTTCTTTAGT 
GAACTGGAGA TGATTCCGCT GCTGGTCACC CTGCCCGAGA AGATCGTGTC CCAGAAGGCC 
TTTATGCTCT CCTACAAATG GATGCTGAGC GATGAGCCCG TCCTGGAGCT GGCTCACATT 
GTGGAGGTCT ATGTGGCCGC CACTTATTTG AGGATCTCTG GTCTGGCGGC GCATTGTTGG 
AAATATTTCG ATGATGAGGA ATACTACAAC GAGGATACGG CATGTGTCCT ATACGTCGAG 
TCGAAGGACA ATCCCGCCAT GGATGTAGTT CGCAATTTGA TGCTGACTAG GATCCGCAAG 
TTTCTGCTCA CCTTTGTGGC CACCAGGGAT TTCCTCGATC TGCCGACCTC CCATCTGATT 
TTTTTGCTCG AATCCGATCA AATCTGTGTG AACACCGAGA TTGAGGTTTT CTTTATAGCT 
GTTCGATGGC TGGGTCACGA TTGGAACAAG AGGAAAGTGC ACGTACGTCG TTTAATGTCC 
TGTATTCGAT TCAACCTGAT GCCATTGTGG TATCTACTAT ATGCGCGACG GGAGGAGGAT 
CACCCGTTGG TGATGAAGCT GATTTTCAAC CCGGAGGTGG AGTACAAGAT CGACGAATCC 
ATATCGAGAA TAACGTCGCG CATGTACGAG GATGCCTTGG AGGGCAATGA CGCACAGAGT 
GAGGAATACG CAAGTGATTC CGGACAGCGA AACTGGATAT GCGATAGCTT GTGCAGCTAT 
TATCACGGCG TCGGTTGTCC CAATACCCGG GAAATTCGTT TCAAACACTT CGAGGATTAC 
CTTTCGGAAT TACAACAATG CTCCAAAGAT CACTGGTCTC AGGTGATATT TCAGGACCCG 
AGTAAAAAGA TTGACTGTTG CAGCACAAGG AAGATAAAAT CGGACGATTT TGTTGCCGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12857 ( NM_001274048.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260977.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12857), mRNA.

Price & Availability↑

CloneID	OFa12548
Clone ID Related Accession (Same CDS sequence)	NM_001274048.1
Accession Version	NM_001274048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGC TAATGAGGCA GAACGGACAG AAATGCGAGA GGCAGGAACT CACCTGCAGC 
GTTTTATTTA CCAATCCCAA GCTTAGTGCC GAGAAGTTAT CCTCCAATGA TGAAATAAAC 
TCCATTGCGA GATTCGTATC GAAGGCGGAG GATCTCGAAA TGCATAGAGT GTTCAGTAAG 
ATATCAAAGA ATGGTGTCGA GGAGACCACG TCTCTGAATC GCTCCCTCTT CGAGTTCGGT 
CATAATTTCG AGGATGCCGA TGGCTGGCTG AAACTGGAGC AGCCTTGCAA GGATCGGCTG 
GCCACGGTGC TGCGCTACAT GTTCGAGTCC CATCTGAAGA CCACTGTGCA GATAGAAATC 
AACAATATGT ACTTCAATTG CCACTTCATC GTGCTGCAGG TGTACTCTCG CTTCTTTAGT 
GAACTGGAGA TGATTCCGCT GCTGGTCACC CTGCCCGAGA AGATCGTGTC CCAGAAGGCC 
TTTATGCTCT CCTACAAATG GATGCTGAGC GATGAGCCCG TCCTGGAGCT GGCTCACATT 
GTGGAGGTCT ATGTGGCCGC CACTTATTTG AGGATCTCTG GTCTGGCGGC GCATTGTTGG 
AAATATTTCG ATGATGAGGA ATACTACAAC GAGGATACGG CATGTGTCCT ATACGTCGAG 
TCGAAGGACA ATCCCGCCAT GGATGTAGTT CGCAATTTGA TGCTGACTAG GATCCGCAAG 
TTTCTGCTCA CCTTTGTGGC CACCAGGGAT TTCCTCGATC TGCCGACCTC CCATCTGATT 
TTTTTGCTCG AATCCGATCA AATCTGTGTG AACACCGAGA TTGAGCTGTT CGATGGCTGG 
GTCACGATTG GAACAAGAGG AAAGTGCACG TACGTCGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260977.1
CDS	47..928
Translation	MEELMRQNGQKCERQELTCSVLFTNPKLSAEKLSSNDEINSIARFVSKAEDLEMHRVFSKISKNGVEETTSLNRSLFEFGHNFEDADGWLKLEQPCKDRLATVLRYMFESHLKTTVQIEINNMYFNCHFIVLQVYSRFFSELEMIPLLVTLPEKIVSQKAFMLSYKWMLSDEPVLELAHIVEVYVAATYLRISGLAAHCWKYFDDEEYYNEDTACVLYVESKDNPAMDVVRNLMLTRIRKFLLTFVATRDFLDLPTSHLIFLLESDQICVNTEIELFDGWVTIGTRGKCTYVV

Target ORF information:

RefSeq Version	NM_001274048.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12857), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274048.1

ORF Insert Sequence:

ATGGAGGAGC TAATGAGGCA GAACGGACAG AAATGCGAGA GGCAGGAACT CACCTGCAGC 
GTTTTATTTA CCAATCCCAA GCTTAGTGCC GAGAAGTTAT CCTCCAATGA TGAAATAAAC 
TCCATTGCGA GATTCGTATC GAAGGCGGAG GATCTCGAAA TGCATAGAGT GTTCAGTAAG 
ATATCAAAGA ATGGTGTCGA GGAGACCACG TCTCTGAATC GCTCCCTCTT CGAGTTCGGT 
CATAATTTCG AGGATGCCGA TGGCTGGCTG AAACTGGAGC AGCCTTGCAA GGATCGGCTG 
GCCACGGTGC TGCGCTACAT GTTCGAGTCC CATCTGAAGA CCACTGTGCA GATAGAAATC 
AACAATATGT ACTTCAATTG CCACTTCATC GTGCTGCAGG TGTACTCTCG CTTCTTTAGT 
GAACTGGAGA TGATTCCGCT GCTGGTCACC CTGCCCGAGA AGATCGTGTC CCAGAAGGCC 
TTTATGCTCT CCTACAAATG GATGCTGAGC GATGAGCCCG TCCTGGAGCT GGCTCACATT 
GTGGAGGTCT ATGTGGCCGC CACTTATTTG AGGATCTCTG GTCTGGCGGC GCATTGTTGG 
AAATATTTCG ATGATGAGGA ATACTACAAC GAGGATACGG CATGTGTCCT ATACGTCGAG 
TCGAAGGACA ATCCCGCCAT GGATGTAGTT CGCAATTTGA TGCTGACTAG GATCCGCAAG 
TTTCTGCTCA CCTTTGTGGC CACCAGGGAT TTCCTCGATC TGCCGACCTC CCATCTGATT 
TTTTTGCTCG AATCCGATCA AATCTGTGTG AACACCGAGA TTGAGCTGTT CGATGGCTGG 
GTCACGATTG GAACAAGAGG AAAGTGCACG TACGTCGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12857 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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