RpI1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RpI1
Entrez Gene ID	36617
Full Name	RNA polymerase I subunit
Synonyms	153129_at,CG10122,DmRPA1,Dmel\CG10122,RPA1
General protein information	Preferred Names RNA polymerase I subunit Names CG10122 gene product from transcript CG10122-RA CG10122-PA RNA polymerase I RpI1-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51B4-51B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079019.3	NP_523743.1	RNA polymerase I subunit

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Neurospora crassa	NCU01638	XP_956006.2
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RpI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RpI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09989	NM_079019.3 Latest version!	Drosophila melanogaster RNA polymerase I subunit (RpI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RpI1 ( NM_079019.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523743.1 Drosophila melanogaster RNA polymerase I subunit (RpI1), mRNA.

Price & Availability↑

CloneID	OFa09989
Clone ID Related Accession (Same CDS sequence)	NM_079019.3
Accession Version	NM_079019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RNA polymerase I subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079019.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGTCTA AACGCGCGAT GGACGTGCAC ATGTTCCCTT CGGACCTGGA ATTCGCCGTG 
TTCACGGACC AGGAGATCCG CAAACTGAGC GTAGTGAAGG TGATCACGGG CATCACGTTC 
GATGCACTGG GACACGCGAT ACCCGGTGGT CTATACGACA TCCGAATGGG CTCCTACGGC 
CGGTGCATGG ATCCCTGTGG GACGTGTCTT AAGTTGCAGG ACTGTCCGGG CCATATGGGC 
CACATAGAGC TAGGCACGCC CGTCTACAAT CCCTTCTTCA TTAAGTTTGT GCAGCGACTT 
CTCTGCATAT TCTGTTTGCA CTGCTACAAG CTGCAGATGA AGGATCACGA ATGTGAGATA 
ATTATGTTAC AGCTGCGCCT AATCGACGCT GGGTACATCA TCGAGGCGCA GGAGTTGGAA 
CTCTTCAAGT CAGAAATTGT GTGCCAAAAC ACCGAAAATC TGGTAGCCAT CAAAAACGGC 
GATATGGTGC ACCCACACAT TGCTGCCATG TATAAACTGC TGGAAAAAAA TGAGAAGAAC 
TCCAGCAACT CCACTAAGAC GAGCTGTTCC CTGCGCACAG CCATTACGCA TTCGGCACTG 
CAGCGACTGG GCAAGAAGTG CAGGCACTGT AACAAGTCTA TGCGTTTCGT GCGTTATATG 
CATCGTAGGC TGGTATTCTA CGTGACTTTG GCAGATATTA AAGAACGCGT TGGCACGGGC 
GCAGAAACCG GTGGTCAGAA TAAAGTGATC TTCGCAGACG AATGTCGTCG TTATCTGCGC 
CAGATATACG CAAATTACCC TGAACTATTA AAGCTGCTAG TGCCTGTTTT GGGGCTAAGC 
AACACGGATT TGACTCAGGG CGATCGTTCG CCGGTGGATC TGTTCTTCAT GGACACGCTT 
CCGGTGACGC CGCCGCGCGC TCGACCACTG AACATGGTCG GTGACATGCT AAAGGGAAAT 
CCCCAAACGG ACATCTACAT AAACATCATT GAAAACAACC ATGTATTGAA TGTGGTACTC 
AAGTACATGA AGGGCGGCCA GGAAAAGCTC ACAGAAGAGG CTAAGGCCGC CTATCAGACC 
CTAAAGGGTG AAACAGCTCA TGAGAAATTG TATACGGCTT GGTTGGCTCT TCAAATGTCG 
GTGGATGTTC TCCTCGATGT GAATATGTCC CGAGAAATGA AATCTGGTGA AGGTCTGAAG 
CAAATCATTG AAAAGAAGAG TGGTCTCATT CGAAGCCATA TGATGGGCAA ACGTGTTAAT 
TACGCCGCTC GTACTGTCAT TACGCCGGAT CCCAATATAA ATGTGGATGA GATTGGCATC 
CCAGATATTT TTGCGAAGAA ACTGTCGTAT CCCGTGCCCG TGACCGAATG GAATGTTACA 
GAGCTGCGCA AAATGGTTAT GAACGGTCCG GACGTGCATC CCGGAGCGAA CTATATACAG 
GACAAGAACG GATTCACCAC ATATATACCG GCGGACAATG CCTCCAAGCG TGAGAGTTTG 
GCCAAGCTAC TTTTGTCGAA TCCCAAGGAT GGCATTAAGA TCGTTCATCG GCATGTTCTC 
AATGGCGACG TCCTACTGCT TAACCGACAG CCCTCGCTGC ATAAGCCATC GATTATGGGT 
CACAAGGCTC GCATTTTGCA TGGTGAGAAG ACTTTCCGTC TACACTACTC AAACTGCAAG 
GCATACAACG CCGATTTTGA TGGCGATGAA ATGAATGCCC ACTATCCGCA GAGTGAGGTG 
GCGAGGGCGG AAGCTTACAA CCTAGTGAAT GTGGCCAGCA ACTATTTGGT GCCCAAGGAC 
GGCACACCGT TGGGTGGTCT CATTCAGGAT CACGTCATTT CGGGTGTAAA GCTGTCGATT 
CGTGGACGAT TCTTTAATCG TGAAGACTAC CAGCAATTGG TGTTTCAGGG ACTATCTCAA 
CTTAAAAAGG ATATCAAGCT GCTGCCACCG ACTATTTTAA AGCCAGCTGT GCTTTGGTCT 
GGAAAGCAGA TCTTGTCCAC CATTATCATC AACATAATTC CCGAGGGATA CGAAAGAATT 
AATCTGGATT CATTTGCCAA GATTGCTGGA AAGAATTGGA ACGTGTCCCG TCCACGACCG 
CCCATTTGTG GCACAAATCC AGAAGGAAAC GACCTAAGCG AAAGCCAAGT GCAAATCAGA 
AACGGAGAAC TGCTTGTTGG TGTTTTGGAT AAACAACAGT ATGGGGCCAC CACGTATGGA 
TTGATTCACT GCATGTATGA GCTATACGGC GGCGATGTGT CAACTTTGCT GCTTACCGCT 
TTCACGAAAG TATTTACCTT TTTCCTGCAA CTGGAAGGTT TTACTTTGGG TGTCAAGGAT 
ATTCTTGTTA CCGACGTAGC AGATCGTAAG AGAAGGAAGA TTATTCGCGA GTGTCGCAAT 
GTGGGAAACA GCGCAGTAGC TGCTGCTCTA GAATTGGAAG ATGAGCCGCC GCACGATGAG 
CTGGTTGAGA AAATGGAAGC GGCATATGTA AAGGATTCGA AGTTCCGAGT GCTTTTGGAT 
CGTAAATACA AATCACTCTT GGATGGCTAC ACAAATGACA TTAATAGTAC TTGTCTGCCC 
AGAGGTCTTA TTACCAAGTT CCCATCAAAC AATCTGCAAT TGATGGTTCT ATCTGGTGCC 
AAGGGATCCA TGGTGAACAC CATGCAAATC TCCTGTCTGC TCGGACAAAT TGAGTTAGAA 
GGAAAGCGAC CGCCTTTGAT GATATCCGGC AAGTCTCTGC CAAGCTTTAC ATCCTTCGAA 
ACCTCGCCCA AGTCTGGAGG TTTTATCGAT GGACGTTTTA TGACCGGCAT TCAGCCGCAG 
GACTTTTTCT TCCATTGCAT GGCTGGTCGT GAAGGTCTCA TTGATACTGC TGTGAAAACA 
TCGCGTTCCG GTTATCTTCA ACGCTGTCTT ATTAAACATC TGGAGGGGCT AAGTGTCCAT 
TATGATCTTA CTGTACGCGA TAGTGACAAT AGTGTGGTTC AGTTCCTATA TGGCGAGGAT 
GGTTTAGACA TTCTGAAGTC AAAGTTTTTC AACGATAAGT TCTGTGCAGA CTTCCTTACA 
CAGAATGCAA CGGCAATTCT ACGCCCTGCT CAACTTCAAT TGATGAAGGA TGAGGAACAG 
CTAGCCAAGG TCCAGCGCCA CGAGAAACAC ATCCGCAGCT GGGAGAAGAA AAAGCCAGCT 
AAGCTACGTG CCGCCTTCAC TCATTTCTCC GAGGAGCTCC GCGAGGAGGT GGAGGTGAAG 
CGACCCAATG AGATTAATAG CAAAACAGGG CGTCGGCGCT TTGATGAGGG CCTCCTAAAG 
TTATGGAAAA AGGCCGATGC AGAGGACAAG GCTTTATACC GCAAGAAGTA CGCCCGTTGT 
CCAGATCCCA CGGTAGCTGT ATACAAGCAG GATCTTTACT ATGGCAGTGT GTCAGAAAGA 
ACGAGGAAAC TCATTACCGA TTATGCTAAG AGGAAGCCAG CACTGAAGGA GACTATAGCC 
GATATTATGC GAGTAAAGAC AATTAAGTCA CTGGCGGCGC CAGGTGAACC AGTGGGTCTG 
ATAGCAGCCC AATCGATAGG AGAACCCTCG ACTCAGATGA CATTGAACAC TTTCCATTTT 
GCGGGTCGTG GTGAGATGAA CGTGACTCTG GGTATTCCTC GACTACGAGA AATTCTCATG 
CTGGCCTCAT CAAACATCAA AACGCCGTCT ATGGATATAC CCATAAAGCC TGGTCAGCAG 
CATCAGGCGG AAAAGCTGCG CATAAATCTA AATTCAGTGA CGTTGGCAAA CCTTTTAGAA 
TACGTCCACG TTAGCACTGG TTTGACTCTG GACCCGGAGC GATCTTATGA GTATGATATG 
CGCTTCCAAT TCCTGCCCAG AGAGGTCTAT AAGGAGGACT ATGGTGTACG ACCCAAACAT 
ATAATCAAAT ACATGCACCA GACCTTTTTT AAACAACTGA TTCGCGCCAT CTTGAAGGTT 
TCAAATGCTT CTAGGACAAC GAAAATAGTT GTAATAGACG ACAAAAAGGA CGCTGATAAA 
GAAGATGATA ACGATTTGGA CAACGGCGAT GAAGTGGGCC GTTCTAAGGC CAAGGCTAAT 
GATGATGATT CATCAGACGA TAACGACGAT GACGATGCAA CTGGAGTAAA GCTTAAGCAA 
CGCAAAACAG ATGAGAAGGA CTACGACGAC CCAGACGACG TCGAGGAACT CCATGATGCT 
AATGACGATG ACGACGAGGC GGAGGATGAG GACGACGAGG AAAAGGGTCA GGATGGTAAT 
GATAATGATG GCGATGATAA AGCGGTAGAG AGGTTGCTGA GCAACGATAT GGTCAAAGCA 
TACACCTACG ACAAGGAGAA CCATTTGTGG TGCCAGGTCA AGTTGAATCT GAGTGTGCGG 
TACCAGAAGC CCGATCTTAC CTCCATAATC CGGGAGTTGG CGGGCAAGAG TGTGGTCCAC 
CAAGTGCAGC ACATCAAGCG GGCCATTATC TACAAAGGCA CCGATGATGA CCAGCTCCTA 
AAGACCGACG GCATTAATAT TGGCGAGATG TTCCAGCACA ACAAGATTCT TGATCTGAAC 
CGCCTTTACT CCAATGACAT TCACGCGATT GCTAGGACGT ACGGCATCGA AGCAGCGTCT 
CAAGTGATCG TTAAGGAAGT GAGCAATGTG TTCAAGGTCT ACGGAATTAC TGTGGATCGC 
CGCCACCTGT CGCTAATTGC CGATTACATG ACCTTCGACG GCACTTTCCA ACCGTTGTCA 
CGCAAGGGCA TGGAGCACTC CTCGTCGCCC CTGCAGCAGA TGTCCTTCGA GTCCAGCTTG 
CAGTTCCTCA AGAGCGCCGC TGGATTCGGC AGAGCGGATG AGCTTAGCTC GCCGTCGAGT 
CGTTTGATGG TTGGCCTCCC GGTGCGGAAT GGTACTGGTG CCTTTGAGTT GCTGACGAAA 
ATTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523743.1
CDS	99..5027
Translation	MGSKRAMDVHMFPSDLEFAVFTDQEIRKLSVVKVITGITFDALGHAIPGGLYDIRMGSYGRCMDPCGTCLKLQDCPGHMGHIELGTPVYNPFFIKFVQRLLCIFCLHCYKLQMKDHECEIIMLQLRLIDAGYIIEAQELELFKSEIVCQNTENLVAIKNGDMVHPHIAAMYKLLEKNEKNSSNSTKTSCSLRTAITHSALQRLGKKCRHCNKSMRFVRYMHRRLVFYVTLADIKERVGTGAETGGQNKVIFADECRRYLRQIYANYPELLKLLVPVLGLSNTDLTQGDRSPVDLFFMDTLPVTPPRARPLNMVGDMLKGNPQTDIYINIIENNHVLNVVLKYMKGGQEKLTEEAKAAYQTLKGETAHEKLYTAWLALQMSVDVLLDVNMSREMKSGEGLKQIIEKKSGLIRSHMMGKRVNYAARTVITPDPNINVDEIGIPDIFAKKLSYPVPVTEWNVTELRKMVMNGPDVHPGANYIQDKNGFTTYIPADNASKRESLAKLLLSNPKDGIKIVHRHVLNGDVLLLNRQPSLHKPSIMGHKARILHGEKTFRLHYSNCKAYNADFDGDEMNAHYPQSEVARAEAYNLVNVASNYLVPKDGTPLGGLIQDHVISGVKLSIRGRFFNREDYQQLVFQGLSQLKKDIKLLPPTILKPAVLWSGKQILSTIIINIIPEGYERINLDSFAKIAGKNWNVSRPRPPICGTNPEGNDLSESQVQIRNGELLVGVLDKQQYGATTYGLIHCMYELYGGDVSTLLLTAFTKVFTFFLQLEGFTLGVKDILVTDVADRKRRKIIRECRNVGNSAVAAALELEDEPPHDELVEKMEAAYVKDSKFRVLLDRKYKSLLDGYTNDINSTCLPRGLITKFPSNNLQLMVLSGAKGSMVNTMQISCLLGQIELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFMTGIQPQDFFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGEDGLDILKSKFFNDKFCADFLTQNATAILRPAQLQLMKDEEQLAKVQRHEKHIRSWEKKKPAKLRAAFTHFSEELREEVEVKRPNEINSKTGRRRFDEGLLKLWKKADAEDKALYRKKYARCPDPTVAVYKQDLYYGSVSERTRKLITDYAKRKPALKETIADIMRVKTIKSLAAPGEPVGLIAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMLASSNIKTPSMDIPIKPGQQHQAEKLRINLNSVTLANLLEYVHVSTGLTLDPERSYEYDMRFQFLPREVYKEDYGVRPKHIIKYMHQTFFKQLIRAILKVSNASRTTKIVVIDDKKDADKEDDNDLDNGDEVGRSKAKANDDDSSDDNDDDDATGVKLKQRKTDEKDYDDPDDVEELHDANDDDDEAEDEDDEEKGQDGNDNDGDDKAVERLLSNDMVKAYTYDKENHLWCQVKLNLSVRYQKPDLTSIIRELAGKSVVHQVQHIKRAIIYKGTDDDQLLKTDGINIGEMFQHNKILDLNRLYSNDIHAIARTYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKGMEHSSSPLQQMSFESSLQFLKSAAGFGRADELSSPSSRLMVGLPVRNGTGAFELLTKIC

Target ORF information:

RefSeq Version	NM_079019.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RNA polymerase I subunit (RpI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079019.3

ORF Insert Sequence:

ATGGGGTCTA AACGCGCGAT GGACGTGCAC ATGTTCCCTT CGGACCTGGA ATTCGCCGTG 
TTCACGGACC AGGAGATCCG CAAACTGAGC GTAGTGAAGG TGATCACGGG CATCACGTTC 
GATGCACTGG GACACGCGAT ACCCGGTGGT CTATACGACA TCCGAATGGG CTCCTACGGC 
CGGTGCATGG ATCCCTGTGG GACGTGTCTT AAGTTGCAGG ACTGTCCGGG CCATATGGGC 
CACATAGAGC TAGGCACGCC CGTCTACAAT CCCTTCTTCA TTAAGTTTGT GCAGCGACTT 
CTCTGCATAT TCTGTTTGCA CTGCTACAAG CTGCAGATGA AGGATCACGA ATGTGAGATA 
ATTATGTTAC AGCTGCGCCT AATCGACGCT GGGTACATCA TCGAGGCGCA GGAGTTGGAA 
CTCTTCAAGT CAGAAATTGT GTGCCAAAAC ACCGAAAATC TGGTAGCCAT CAAAAACGGC 
GATATGGTGC ACCCACACAT TGCTGCCATG TATAAACTGC TGGAAAAAAA TGAGAAGAAC 
TCCAGCAACT CCACTAAGAC GAGCTGTTCC CTGCGCACAG CCATTACGCA TTCGGCACTG 
CAGCGACTGG GCAAGAAGTG CAGGCACTGT AACAAGTCTA TGCGTTTCGT GCGTTATATG 
CATCGTAGGC TGGTATTCTA CGTGACTTTG GCAGATATTA AAGAACGCGT TGGCACGGGC 
GCAGAAACCG GTGGTCAGAA TAAAGTGATC TTCGCAGACG AATGTCGTCG TTATCTGCGC 
CAGATATACG CAAATTACCC TGAACTATTA AAGCTGCTAG TGCCTGTTTT GGGGCTAAGC 
AACACGGATT TGACTCAGGG CGATCGTTCG CCGGTGGATC TGTTCTTCAT GGACACGCTT 
CCGGTGACGC CGCCGCGCGC TCGACCACTG AACATGGTCG GTGACATGCT AAAGGGAAAT 
CCCCAAACGG ACATCTACAT AAACATCATT GAAAACAACC ATGTATTGAA TGTGGTACTC 
AAGTACATGA AGGGCGGCCA GGAAAAGCTC ACAGAAGAGG CTAAGGCCGC CTATCAGACC 
CTAAAGGGTG AAACAGCTCA TGAGAAATTG TATACGGCTT GGTTGGCTCT TCAAATGTCG 
GTGGATGTTC TCCTCGATGT GAATATGTCC CGAGAAATGA AATCTGGTGA AGGTCTGAAG 
CAAATCATTG AAAAGAAGAG TGGTCTCATT CGAAGCCATA TGATGGGCAA ACGTGTTAAT 
TACGCCGCTC GTACTGTCAT TACGCCGGAT CCCAATATAA ATGTGGATGA GATTGGCATC 
CCAGATATTT TTGCGAAGAA ACTGTCGTAT CCCGTGCCCG TGACCGAATG GAATGTTACA 
GAGCTGCGCA AAATGGTTAT GAACGGTCCG GACGTGCATC CCGGAGCGAA CTATATACAG 
GACAAGAACG GATTCACCAC ATATATACCG GCGGACAATG CCTCCAAGCG TGAGAGTTTG 
GCCAAGCTAC TTTTGTCGAA TCCCAAGGAT GGCATTAAGA TCGTTCATCG GCATGTTCTC 
AATGGCGACG TCCTACTGCT TAACCGACAG CCCTCGCTGC ATAAGCCATC GATTATGGGT 
CACAAGGCTC GCATTTTGCA TGGTGAGAAG ACTTTCCGTC TACACTACTC AAACTGCAAG 
GCATACAACG CCGATTTTGA TGGCGATGAA ATGAATGCCC ACTATCCGCA GAGTGAGGTG 
GCGAGGGCGG AAGCTTACAA CCTAGTGAAT GTGGCCAGCA ACTATTTGGT GCCCAAGGAC 
GGCACACCGT TGGGTGGTCT CATTCAGGAT CACGTCATTT CGGGTGTAAA GCTGTCGATT 
CGTGGACGAT TCTTTAATCG TGAAGACTAC CAGCAATTGG TGTTTCAGGG ACTATCTCAA 
CTTAAAAAGG ATATCAAGCT GCTGCCACCG ACTATTTTAA AGCCAGCTGT GCTTTGGTCT 
GGAAAGCAGA TCTTGTCCAC CATTATCATC AACATAATTC CCGAGGGATA CGAAAGAATT 
AATCTGGATT CATTTGCCAA GATTGCTGGA AAGAATTGGA ACGTGTCCCG TCCACGACCG 
CCCATTTGTG GCACAAATCC AGAAGGAAAC GACCTAAGCG AAAGCCAAGT GCAAATCAGA 
AACGGAGAAC TGCTTGTTGG TGTTTTGGAT AAACAACAGT ATGGGGCCAC CACGTATGGA 
TTGATTCACT GCATGTATGA GCTATACGGC GGCGATGTGT CAACTTTGCT GCTTACCGCT 
TTCACGAAAG TATTTACCTT TTTCCTGCAA CTGGAAGGTT TTACTTTGGG TGTCAAGGAT 
ATTCTTGTTA CCGACGTAGC AGATCGTAAG AGAAGGAAGA TTATTCGCGA GTGTCGCAAT 
GTGGGAAACA GCGCAGTAGC TGCTGCTCTA GAATTGGAAG ATGAGCCGCC GCACGATGAG 
CTGGTTGAGA AAATGGAAGC GGCATATGTA AAGGATTCGA AGTTCCGAGT GCTTTTGGAT 
CGTAAATACA AATCACTCTT GGATGGCTAC ACAAATGACA TTAATAGTAC TTGTCTGCCC 
AGAGGTCTTA TTACCAAGTT CCCATCAAAC AATCTGCAAT TGATGGTTCT ATCTGGTGCC 
AAGGGATCCA TGGTGAACAC CATGCAAATC TCCTGTCTGC TCGGACAAAT TGAGTTAGAA 
GGAAAGCGAC CGCCTTTGAT GATATCCGGC AAGTCTCTGC CAAGCTTTAC ATCCTTCGAA 
ACCTCGCCCA AGTCTGGAGG TTTTATCGAT GGACGTTTTA TGACCGGCAT TCAGCCGCAG 
GACTTTTTCT TCCATTGCAT GGCTGGTCGT GAAGGTCTCA TTGATACTGC TGTGAAAACA 
TCGCGTTCCG GTTATCTTCA ACGCTGTCTT ATTAAACATC TGGAGGGGCT AAGTGTCCAT 
TATGATCTTA CTGTACGCGA TAGTGACAAT AGTGTGGTTC AGTTCCTATA TGGCGAGGAT 
GGTTTAGACA TTCTGAAGTC AAAGTTTTTC AACGATAAGT TCTGTGCAGA CTTCCTTACA 
CAGAATGCAA CGGCAATTCT ACGCCCTGCT CAACTTCAAT TGATGAAGGA TGAGGAACAG 
CTAGCCAAGG TCCAGCGCCA CGAGAAACAC ATCCGCAGCT GGGAGAAGAA AAAGCCAGCT 
AAGCTACGTG CCGCCTTCAC TCATTTCTCC GAGGAGCTCC GCGAGGAGGT GGAGGTGAAG 
CGACCCAATG AGATTAATAG CAAAACAGGG CGTCGGCGCT TTGATGAGGG CCTCCTAAAG 
TTATGGAAAA AGGCCGATGC AGAGGACAAG GCTTTATACC GCAAGAAGTA CGCCCGTTGT 
CCAGATCCCA CGGTAGCTGT ATACAAGCAG GATCTTTACT ATGGCAGTGT GTCAGAAAGA 
ACGAGGAAAC TCATTACCGA TTATGCTAAG AGGAAGCCAG CACTGAAGGA GACTATAGCC 
GATATTATGC GAGTAAAGAC AATTAAGTCA CTGGCGGCGC CAGGTGAACC AGTGGGTCTG 
ATAGCAGCCC AATCGATAGG AGAACCCTCG ACTCAGATGA CATTGAACAC TTTCCATTTT 
GCGGGTCGTG GTGAGATGAA CGTGACTCTG GGTATTCCTC GACTACGAGA AATTCTCATG 
CTGGCCTCAT CAAACATCAA AACGCCGTCT ATGGATATAC CCATAAAGCC TGGTCAGCAG 
CATCAGGCGG AAAAGCTGCG CATAAATCTA AATTCAGTGA CGTTGGCAAA CCTTTTAGAA 
TACGTCCACG TTAGCACTGG TTTGACTCTG GACCCGGAGC GATCTTATGA GTATGATATG 
CGCTTCCAAT TCCTGCCCAG AGAGGTCTAT AAGGAGGACT ATGGTGTACG ACCCAAACAT 
ATAATCAAAT ACATGCACCA GACCTTTTTT AAACAACTGA TTCGCGCCAT CTTGAAGGTT 
TCAAATGCTT CTAGGACAAC GAAAATAGTT GTAATAGACG ACAAAAAGGA CGCTGATAAA 
GAAGATGATA ACGATTTGGA CAACGGCGAT GAAGTGGGCC GTTCTAAGGC CAAGGCTAAT 
GATGATGATT CATCAGACGA TAACGACGAT GACGATGCAA CTGGAGTAAA GCTTAAGCAA 
CGCAAAACAG ATGAGAAGGA CTACGACGAC CCAGACGACG TCGAGGAACT CCATGATGCT 
AATGACGATG ACGACGAGGC GGAGGATGAG GACGACGAGG AAAAGGGTCA GGATGGTAAT 
GATAATGATG GCGATGATAA AGCGGTAGAG AGGTTGCTGA GCAACGATAT GGTCAAAGCA 
TACACCTACG ACAAGGAGAA CCATTTGTGG TGCCAGGTCA AGTTGAATCT GAGTGTGCGG 
TACCAGAAGC CCGATCTTAC CTCCATAATC CGGGAGTTGG CGGGCAAGAG TGTGGTCCAC 
CAAGTGCAGC ACATCAAGCG GGCCATTATC TACAAAGGCA CCGATGATGA CCAGCTCCTA 
AAGACCGACG GCATTAATAT TGGCGAGATG TTCCAGCACA ACAAGATTCT TGATCTGAAC 
CGCCTTTACT CCAATGACAT TCACGCGATT GCTAGGACGT ACGGCATCGA AGCAGCGTCT 
CAAGTGATCG TTAAGGAAGT GAGCAATGTG TTCAAGGTCT ACGGAATTAC TGTGGATCGC 
CGCCACCTGT CGCTAATTGC CGATTACATG ACCTTCGACG GCACTTTCCA ACCGTTGTCA 
CGCAAGGGCA TGGAGCACTC CTCGTCGCCC CTGCAGCAGA TGTCCTTCGA GTCCAGCTTG 
CAGTTCCTCA AGAGCGCCGC TGGATTCGGC AGAGCGGATG AGCTTAGCTC GCCGTCGAGT 
CGTTTGATGG TTGGCCTCCC GGTGCGGAAT GGTACTGGTG CCTTTGAGTT GCTGACGAAA 
ATTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RpI1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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