LamC cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LamC
Entrez Gene ID	36615
Full Name	Lamin C
Synonyms	CG10119,DmC,Dmel\CG10119,G-IF,LAMC,lamC,pG-IF
General protein information	Preferred Names Lamin C Names CG10119 gene product from transcript CG10119-RA CG10119-PA CG10119-PB G intermediate filament LamC-PA LamC-PB LaminC lamin A lamin C lamin DmC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51B1-51B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079018.3	NP_523742.2	lamin C, isoform A
NM_001274045.1	NP_001260974.1	lamin C, isoform B

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ifd-1	NP_001024830.1
Caenorhabditis elegans (roundworm)	ifa-3	NP_510649.3
Caenorhabditis elegans (roundworm)	ifa-4	NP_508836.4
Pan troglodytes (chimpanzee)	LMNB2	XP_524044.4
	LMNB2	XP_002801064.1
Gallus gallus (chicken)	LMNB2	NP_990616.1
Drosophila melanogaster (fruit fly)	LamC	NP_523742.2
Caenorhabditis elegans (roundworm)	ifd-2	NP_508160.1
Xenopus tropicalis (tropical clawed frog)	lmnb2	XP_002940085.1
Homo sapiens (human)	LMNB2	NP_116126.3
Bos taurus (cattle)	LMNB2	NP_001263282.1
Mus musculus (house mouse)	Lmnb2	NP_034852.2
Rattus norvegicus (Norway rat)	Lmnb2	XP_216850.6
Danio rerio (zebrafish)	lmnb2	NP_571077.2
Drosophila melanogaster (fruit fly)	Lam	NP_001245892.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011938	XP_320593.4
Caenorhabditis elegans (roundworm)	ifa-1	NP_001076764.1
Caenorhabditis elegans (roundworm)	ifb-1	NP_495136.1
Caenorhabditis elegans (roundworm)	F10C1.8	NP_495132.2
Caenorhabditis elegans (roundworm)	ifp-1	NP_509628.1
Caenorhabditis elegans (roundworm)	mua-6	NP_510648.1
Caenorhabditis elegans (roundworm)	ifb-2	NP_495134.1
Canis lupus familiaris (dog)	LMNB2	XP_542188.3
Caenorhabditis elegans (roundworm)	ifc-1	NP_503783.1
Caenorhabditis elegans (roundworm)	ifc-2	NP_001256975.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Human progeroid syndrome-modeled LamC mutations induced premature aging in adult flight muscles, including decreased levels of specific mitochondrial protein transcripts (RNA) and progressive mitochondrial degradation. They also induced the accelerated appearance of other phenotypes associated with aging.
Title: Lamin Mutations Accelerate Aging via Defective Export of Mitochondrial mRNAs through Nuclear Envelope Budding.

The critical function of A-type lamin C proteins in Drosophila musculature is to maintain proper function and morphology of tendon cells.
Title: Loss of Drosophila A-type lamin C initially causes tendon abnormality including disintegration of cytoskeleton and nuclear lamina in muscular defects.

Study finds that C-terminal fragments of DFz2 accumulate in nuclear foci in association with large RNA granules localizing to the space between the inner
Title: Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling.

of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C
Title: The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C.

larval muscle-specific expression of a truncated form of Lamin C lacking the N-terminal head (Lamin C DeltaN) caused muscle defects and semi-lethality, with adult 'escapers' possessing malformed legs.
Title: The role of Drosophila Lamin C in muscle function and gene expression.

The following LamC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LamC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09987	NM_079018.3 Latest version!	Drosophila melanogaster lamin C, transcript variant A (LamC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12110	NM_001274045.1 Latest version!	Drosophila melanogaster lamin C, transcript variant B (LamC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LamC ( NM_079018.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523742.2 Drosophila melanogaster lamin C, transcript variant A (LamC), mRNA.

Price & Availability↑

CloneID	OFa09987
Clone ID Related Accession (Same CDS sequence)	NM_079018.3
Accession Version	NM_079018.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lamin C, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_079018.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCAC GCCGCGTCAC ATTGAACACA CGCGTTTCGC GCGCCTCCAC CTCCACGCCG 
GTGGGCGGGG CATCCACCTC GTCAAGGGTG GGCGCCACCT CACCCACCTC GCCCACGCGC 
ACCAGCCGGC AGCAGGAGAA GGAGGAACTG CAGCATTTGA ACGATCGCCT GGCCTGCTAC 
ATCGATCGCA TGCGCAACCT GGAGAACGAG AACAGCCGGC TCACCCAGGA ACTGAATCTC 
GCCCAGGATA CCGTCAACAG GGAGACCTCT AATCTGAAGG CGGTCTATGA GAAGGAGCTG 
GCCGCCGCTC GCAAGCTGCT CGATGAGACC GCCAAAGAGA AGGCCAAGCT TGAGATCGAT 
ATCAAGCGTC TGTGGGAGGA GAACGACGAC TTGAAGCCCA GACTCGACAA AAAGACGAAG 
GAGGCTACCG TGGCAGAGAA CAATGCCCGC CTGTATGAGA ACCGCTACAA CGAGGTGAAT 
GGCAAGTACA ACCAATCGTT GGCCGATCGC AAGAAGTTCG AGGATCAGGC CAAGGAGCTC 
GCTCTGGAGA ACGAACGCCT GCGCCGCCAA CTGGATGATC TTCGCAAGCA GCTGGAGGCG 
GAGACGCTGG CCCGCGTCGA TCTGGAGAAC CAGAACCAGA GCCTGCGCGA GGAGCTGGCC 
TTCAAGGATC AGGTGCACAC CCAGGAGCTC ACCGAGACCC GATCCCGTCG CCAGATCGAG 
ATCAGCGAGA TCGATGGCCG ACTGTCGCGT CAATACGAGG CCAAGCTGCA GCAATCGCTC 
CAGGAACTGC GTGATCAGTA CGAGGGTCAA ATGCGCATCA ATCGCGAGGA GATCGAGCTG 
CTGTACGACA ATGAGATCCA GAACCTCAAG GCTGCTGCCA ATAGGGCTGC CCAGGGATCC 
GCTCTTGCCA CCGAGGAAGT CCGTCTCATG CGCACCAAGA TCGATGGCTT GAACGCCAAG 
CTACAGAATC TGGAGGACAC CAATGCTGGC TTGAATGCTC GCATTCGGGA GCTGGAGAAC 
CTCTTGGACA CGGAGCGCCA GCGCCACAAC CAGTACATCG CCTCCTTGGA AGCCGAACTA 
CAGCGTATGC GCGACGAGAT GGCTCACCAG CTGCAGGAGT ACCAGGGTCT GATGGACATC 
AAGGTGTCGT TGGATCTCGA AATTGCCGCT TACGACAAAC TGCTGTGCGG TGAGGAGCGT 
CGCCTGAACA TCGAGTCGCC CGGACGCCCG ACTACCGACT CCGGTATCTC CAGCAATGGC 
AGCCACTTGA CCGCCAGTGC CAGTTCCCGA TCCGGCAGGG TCACGCCCAG TGGCCGTCGC 
TCGGCTACAC CTGGCATCTC TGGATCCAGC GCCGTGAAGC GTCGCCGCAC CGTGATCGAT 
GAGAGCGAGG ATCGCACTCT GTCCGAGTAC TCGGTGAATG CCGCCGCCAA GGGAGATCTG 
GAGATCATCG AGGCGGACGT TGAGGGACGC TTCATCAAGC TGCACAACAA GGGAACCGAG 
GAGATCAATC TGACTGGCTG GCAGCTAACC CGCATTGCCG GCGACGAGGA ACTGGCCTTC 
AAGTTCTCGC GCGGATCGAA GGTACTCGGC GGTGCTAGTG TAACCATTTG GTCCGTCGAT 
GCTGGTACCG CACATGATCC ACCCAACAAT CTGGTGATGA AGAAGAAGTG GCCGGTGGCC 
AACTCGATGC GATCGGTGCT GGCCAATGCC GACAAAGAGG ATGTTGCCAG CTACGACCGT 
GTCCGTGCTA ACGTTTCCAG CCACACCTCG CGGCATCGCA GCAGTGGCAC GCCCAGTACG 
GGCTTTACCC TCGGTTCCGG AGCTGGCTCC ACTGGCGTGA GGAGCCTCTT CTCCCTGCTC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523742.2
CDS	147..2012
Translation	MSARRVTLNTRVSRASTSTPVGGASTSSRVGATSPTSPTRTSRQQEKEELQHLNDRLACYIDRMRNLENENSRLTQELNLAQDTVNRETSNLKAVYEKELAAARKLLDETAKEKAKLEIDIKRLWEENDDLKPRLDKKTKEATVAENNARLYENRYNEVNGKYNQSLADRKKFEDQAKELALENERLRRQLDDLRKQLEAETLARVDLENQNQSLREELAFKDQVHTQELTETRSRRQIEISEIDGRLSRQYEAKLQQSLQELRDQYEGQMRINREEIELLYDNEIQNLKAAANRAAQGSALATEEVRLMRTKIDGLNAKLQNLEDTNAGLNARIRELENLLDTERQRHNQYIASLEAELQRMRDEMAHQLQEYQGLMDIKVSLDLEIAAYDKLLCGEERRLNIESPGRPTTDSGISSNGSHLTASASSRSGRVTPSGRRSATPGISGSSAVKRRRTVIDESEDRTLSEYSVNAAAKGDLEIIEADVEGRFIKLHNKGTEEINLTGWQLTRIAGDEELAFKFSRGSKVLGGASVTIWSVDAGTAHDPPNNLVMKKKWPVANSMRSVLANADKEDVASYDRVRANVSSHTSRHRSSGTPSTGFTLGSGAGSTGVRSLFSLLF

Target ORF information:

RefSeq Version	NM_079018.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lamin C, transcript variant A (LamC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079018.3

ORF Insert Sequence:

ATGTCAGCAC GCCGCGTCAC ATTGAACACA CGCGTTTCGC GCGCCTCCAC CTCCACGCCG 
GTGGGCGGGG CATCCACCTC GTCAAGGGTG GGCGCCACCT CACCCACCTC GCCCACGCGC 
ACCAGCCGGC AGCAGGAGAA GGAGGAACTG CAGCATTTGA ACGATCGCCT GGCCTGCTAC 
ATCGATCGCA TGCGCAACCT GGAGAACGAG AACAGCCGGC TCACCCAGGA ACTGAATCTC 
GCCCAGGATA CCGTCAACAG GGAGACCTCT AATCTGAAGG CGGTCTATGA GAAGGAGCTG 
GCCGCCGCTC GCAAGCTGCT CGATGAGACC GCCAAAGAGA AGGCCAAGCT TGAGATCGAT 
ATCAAGCGTC TGTGGGAGGA GAACGACGAC TTGAAGCCCA GACTCGACAA AAAGACGAAG 
GAGGCTACCG TGGCAGAGAA CAATGCCCGC CTGTATGAGA ACCGCTACAA CGAGGTGAAT 
GGCAAGTACA ACCAATCGTT GGCCGATCGC AAGAAGTTCG AGGATCAGGC CAAGGAGCTC 
GCTCTGGAGA ACGAACGCCT GCGCCGCCAA CTGGATGATC TTCGCAAGCA GCTGGAGGCG 
GAGACGCTGG CCCGCGTCGA TCTGGAGAAC CAGAACCAGA GCCTGCGCGA GGAGCTGGCC 
TTCAAGGATC AGGTGCACAC CCAGGAGCTC ACCGAGACCC GATCCCGTCG CCAGATCGAG 
ATCAGCGAGA TCGATGGCCG ACTGTCGCGT CAATACGAGG CCAAGCTGCA GCAATCGCTC 
CAGGAACTGC GTGATCAGTA CGAGGGTCAA ATGCGCATCA ATCGCGAGGA GATCGAGCTG 
CTGTACGACA ATGAGATCCA GAACCTCAAG GCTGCTGCCA ATAGGGCTGC CCAGGGATCC 
GCTCTTGCCA CCGAGGAAGT CCGTCTCATG CGCACCAAGA TCGATGGCTT GAACGCCAAG 
CTACAGAATC TGGAGGACAC CAATGCTGGC TTGAATGCTC GCATTCGGGA GCTGGAGAAC 
CTCTTGGACA CGGAGCGCCA GCGCCACAAC CAGTACATCG CCTCCTTGGA AGCCGAACTA 
CAGCGTATGC GCGACGAGAT GGCTCACCAG CTGCAGGAGT ACCAGGGTCT GATGGACATC 
AAGGTGTCGT TGGATCTCGA AATTGCCGCT TACGACAAAC TGCTGTGCGG TGAGGAGCGT 
CGCCTGAACA TCGAGTCGCC CGGACGCCCG ACTACCGACT CCGGTATCTC CAGCAATGGC 
AGCCACTTGA CCGCCAGTGC CAGTTCCCGA TCCGGCAGGG TCACGCCCAG TGGCCGTCGC 
TCGGCTACAC CTGGCATCTC TGGATCCAGC GCCGTGAAGC GTCGCCGCAC CGTGATCGAT 
GAGAGCGAGG ATCGCACTCT GTCCGAGTAC TCGGTGAATG CCGCCGCCAA GGGAGATCTG 
GAGATCATCG AGGCGGACGT TGAGGGACGC TTCATCAAGC TGCACAACAA GGGAACCGAG 
GAGATCAATC TGACTGGCTG GCAGCTAACC CGCATTGCCG GCGACGAGGA ACTGGCCTTC 
AAGTTCTCGC GCGGATCGAA GGTACTCGGC GGTGCTAGTG TAACCATTTG GTCCGTCGAT 
GCTGGTACCG CACATGATCC ACCCAACAAT CTGGTGATGA AGAAGAAGTG GCCGGTGGCC 
AACTCGATGC GATCGGTGCT GGCCAATGCC GACAAAGAGG ATGTTGCCAG CTACGACCGT 
GTCCGTGCTA ACGTTTCCAG CCACACCTCG CGGCATCGCA GCAGTGGCAC GCCCAGTACG 
GGCTTTACCC TCGGTTCCGG AGCTGGCTCC ACTGGCGTGA GGAGCCTCTT CTCCCTGCTC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LamC ( NM_001274045.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260974.1 Drosophila melanogaster lamin C, transcript variant B (LamC), mRNA.

Price & Availability↑

CloneID	OFa12110
Clone ID Related Accession (Same CDS sequence)	NM_001274045.1
Accession Version	NM_001274045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lamin C, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCAC GCCGCGTCAC ATTGAACACA CGCGTTTCGC GCGCCTCCAC CTCCACGCCG 
GTGGGCGGGG CATCCACCTC GTCAAGGGTG GGCGCCACCT CACCCACCTC GCCCACGCGC 
ACCAGCCGGC AGCAGGAGAA GGAGGAACTG CAGCATTTGA ACGATCGCCT GGCCTGCTAC 
ATCGATCGCA TGCGCAACCT GGAGAACGAG AACAGCCGGC TCACCCAGGA ACTGAATCTC 
GCCCAGGATA CCGTCAACAG GGAGACCTCT AATCTGAAGG CGGTCTATGA GAAGGAGCTG 
GCCGCCGCTC GCAAGCTGCT CGATGAGACC GCCAAAGAGA AGGCCAAGCT TGAGATCGAT 
ATCAAGCGTC TGTGGGAGGA GAACGACGAC TTGAAGCCCA GACTCGACAA AAAGACGAAG 
GAGGCTACCG TGGCAGAGAA CAATGCCCGC CTGTATGAGA ACCGCTACAA CGAGGTGAAT 
GGCAAGTACA ACCAATCGTT GGCCGATCGC AAGAAGTTCG AGGATCAGGC CAAGGAGCTC 
GCTCTGGAGA ACGAACGCCT GCGCCGCCAA CTGGATGATC TTCGCAAGCA GCTGGAGGCG 
GAGACGCTGG CCCGCGTCGA TCTGGAGAAC CAGAACCAGA GCCTGCGCGA GGAGCTGGCC 
TTCAAGGATC AGGTGCACAC CCAGGAGCTC ACCGAGACCC GATCCCGTCG CCAGATCGAG 
ATCAGCGAGA TCGATGGCCG ACTGTCGCGT CAATACGAGG CCAAGCTGCA GCAATCGCTC 
CAGGAACTGC GTGATCAGTA CGAGGGTCAA ATGCGCATCA ATCGCGAGGA GATCGAGCTG 
CTGTACGACA ATGAGATCCA GAACCTCAAG GCTGCTGCCA ATAGGGCTGC CCAGGGATCC 
GCTCTTGCCA CCGAGGAAGT CCGTCTCATG CGCACCAAGA TCGATGGCTT GAACGCCAAG 
CTACAGAATC TGGAGGACAC CAATGCTGGC TTGAATGCTC GCATTCGGGA GCTGGAGAAC 
CTCTTGGACA CGGAGCGCCA GCGCCACAAC CAGTACATCG CCTCCTTGGA AGCCGAACTA 
CAGCGTATGC GCGACGAGAT GGCTCACCAG CTGCAGGAGT ACCAGGGTCT GATGGACATC 
AAGGTGTCGT TGGATCTCGA AATTGCCGCT TACGACAAAC TGCTGTGCGG TGAGGAGCGT 
CGCCTGAACA TCGAGTCGCC CGGACGCCCG ACTACCGACT CCGGTATCTC CAGCAATGGC 
AGCCACTTGA CCGCCAGTGC CAGTTCCCGA TCCGGCAGGG TCACGCCCAG TGGCCGTCGC 
TCGGCTACAC CTGGCATCTC TGGATCCAGC GCCGTGAAGC GTCGCCGCAC CGTGATCGAT 
GAGAGCGAGG ATCGCACTCT GTCCGAGTAC TCGGTGAATG CCGCCGCCAA GGGAGATCTG 
GAGATCATCG AGGCGGACGT TGAGGGACGC TTCATCAAGC TGCACAACAA GGGAACCGAG 
GAGATCAATC TGACTGGCTG GCAGCTAACC CGCATTGCCG GCGACGAGGA ACTGGCCTTC 
AAGTTCTCGC GCGGATCGAA GGTACTCGGC GGTGCTAGTG TAACCATTTG GTCCGTCGAT 
GCTGGTACCG CACATGATCC ACCCAACAAT CTGGTGATGA AGAAGAAGTG GCCGGTGGCC 
AACTCGATGC GATCGGTGCT GGCCAATGCC GACAAAGAGG TGAGAGTCTT GTCCATATAC 
AACACATGCG GCACTTGCGA CATCTGCCGC CACTGCAGAC ACCTCGAATT CGTGCTCGAC 
CAGATGTTGC CAGCTACGAC CGTGTCCGTG CTAACGTTTC CAGCCACACC TCGCGGCATC 
GCAGCAGTGG CACGCCCAGT ACGGGCTTTA CCCTCGGTTC CGGAGCTGGC TCCACTGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260974.1
CDS	147..2069
Translation	MSARRVTLNTRVSRASTSTPVGGASTSSRVGATSPTSPTRTSRQQEKEELQHLNDRLACYIDRMRNLENENSRLTQELNLAQDTVNRETSNLKAVYEKELAAARKLLDETAKEKAKLEIDIKRLWEENDDLKPRLDKKTKEATVAENNARLYENRYNEVNGKYNQSLADRKKFEDQAKELALENERLRRQLDDLRKQLEAETLARVDLENQNQSLREELAFKDQVHTQELTETRSRRQIEISEIDGRLSRQYEAKLQQSLQELRDQYEGQMRINREEIELLYDNEIQNLKAAANRAAQGSALATEEVRLMRTKIDGLNAKLQNLEDTNAGLNARIRELENLLDTERQRHNQYIASLEAELQRMRDEMAHQLQEYQGLMDIKVSLDLEIAAYDKLLCGEERRLNIESPGRPTTDSGISSNGSHLTASASSRSGRVTPSGRRSATPGISGSSAVKRRRTVIDESEDRTLSEYSVNAAAKGDLEIIEADVEGRFIKLHNKGTEEINLTGWQLTRIAGDEELAFKFSRGSKVLGGASVTIWSVDAGTAHDPPNNLVMKKKWPVANSMRSVLANADKEVRVLSIYNTCGTCDICRHCRHLEFVLDQMLPATTVSVLTFPATPRGIAAVARPVRALPSVPELAPLA

Target ORF information:

RefSeq Version	NM_001274045.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lamin C, transcript variant B (LamC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274045.1

ORF Insert Sequence:

ATGTCAGCAC GCCGCGTCAC ATTGAACACA CGCGTTTCGC GCGCCTCCAC CTCCACGCCG 
GTGGGCGGGG CATCCACCTC GTCAAGGGTG GGCGCCACCT CACCCACCTC GCCCACGCGC 
ACCAGCCGGC AGCAGGAGAA GGAGGAACTG CAGCATTTGA ACGATCGCCT GGCCTGCTAC 
ATCGATCGCA TGCGCAACCT GGAGAACGAG AACAGCCGGC TCACCCAGGA ACTGAATCTC 
GCCCAGGATA CCGTCAACAG GGAGACCTCT AATCTGAAGG CGGTCTATGA GAAGGAGCTG 
GCCGCCGCTC GCAAGCTGCT CGATGAGACC GCCAAAGAGA AGGCCAAGCT TGAGATCGAT 
ATCAAGCGTC TGTGGGAGGA GAACGACGAC TTGAAGCCCA GACTCGACAA AAAGACGAAG 
GAGGCTACCG TGGCAGAGAA CAATGCCCGC CTGTATGAGA ACCGCTACAA CGAGGTGAAT 
GGCAAGTACA ACCAATCGTT GGCCGATCGC AAGAAGTTCG AGGATCAGGC CAAGGAGCTC 
GCTCTGGAGA ACGAACGCCT GCGCCGCCAA CTGGATGATC TTCGCAAGCA GCTGGAGGCG 
GAGACGCTGG CCCGCGTCGA TCTGGAGAAC CAGAACCAGA GCCTGCGCGA GGAGCTGGCC 
TTCAAGGATC AGGTGCACAC CCAGGAGCTC ACCGAGACCC GATCCCGTCG CCAGATCGAG 
ATCAGCGAGA TCGATGGCCG ACTGTCGCGT CAATACGAGG CCAAGCTGCA GCAATCGCTC 
CAGGAACTGC GTGATCAGTA CGAGGGTCAA ATGCGCATCA ATCGCGAGGA GATCGAGCTG 
CTGTACGACA ATGAGATCCA GAACCTCAAG GCTGCTGCCA ATAGGGCTGC CCAGGGATCC 
GCTCTTGCCA CCGAGGAAGT CCGTCTCATG CGCACCAAGA TCGATGGCTT GAACGCCAAG 
CTACAGAATC TGGAGGACAC CAATGCTGGC TTGAATGCTC GCATTCGGGA GCTGGAGAAC 
CTCTTGGACA CGGAGCGCCA GCGCCACAAC CAGTACATCG CCTCCTTGGA AGCCGAACTA 
CAGCGTATGC GCGACGAGAT GGCTCACCAG CTGCAGGAGT ACCAGGGTCT GATGGACATC 
AAGGTGTCGT TGGATCTCGA AATTGCCGCT TACGACAAAC TGCTGTGCGG TGAGGAGCGT 
CGCCTGAACA TCGAGTCGCC CGGACGCCCG ACTACCGACT CCGGTATCTC CAGCAATGGC 
AGCCACTTGA CCGCCAGTGC CAGTTCCCGA TCCGGCAGGG TCACGCCCAG TGGCCGTCGC 
TCGGCTACAC CTGGCATCTC TGGATCCAGC GCCGTGAAGC GTCGCCGCAC CGTGATCGAT 
GAGAGCGAGG ATCGCACTCT GTCCGAGTAC TCGGTGAATG CCGCCGCCAA GGGAGATCTG 
GAGATCATCG AGGCGGACGT TGAGGGACGC TTCATCAAGC TGCACAACAA GGGAACCGAG 
GAGATCAATC TGACTGGCTG GCAGCTAACC CGCATTGCCG GCGACGAGGA ACTGGCCTTC 
AAGTTCTCGC GCGGATCGAA GGTACTCGGC GGTGCTAGTG TAACCATTTG GTCCGTCGAT 
GCTGGTACCG CACATGATCC ACCCAACAAT CTGGTGATGA AGAAGAAGTG GCCGGTGGCC 
AACTCGATGC GATCGGTGCT GGCCAATGCC GACAAAGAGG TGAGAGTCTT GTCCATATAC 
AACACATGCG GCACTTGCGA CATCTGCCGC CACTGCAGAC ACCTCGAATT CGTGCTCGAC 
CAGATGTTGC CAGCTACGAC CGTGTCCGTG CTAACGTTTC CAGCCACACC TCGCGGCATC 
GCAGCAGTGG CACGCCCAGT ACGGGCTTTA CCCTCGGTTC CGGAGCTGGC TCCACTGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

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LamC cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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