ttv cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ttv
Entrez Gene ID	36614
Full Name	tout-velu
Synonyms	CG10117,DEXT1,Dmel\CG10117,Ext1,P1.15,Ttv,dExt1,l(2)00681,l(2)05282,l(2)SH0631,l(2)SH2 0631,l(2)k00115,l(2)k03617
General protein information	Preferred Names tout-velu Names CG10117 gene product from transcript CG10117-RB CG10117-PA CG10117-PB CG10117-PC lethal (2) SH0631 tout velu tout-velo toutvelu ttv-PA ttv-PB ttv-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51A7-51B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057883.3	NP_477231.1	tout-velu, isoform A
NM_001274042.1	NP_001260971.1	tout-velu, isoform B
NM_001369957.1	NP_001356963.1	tout-velu, isoform D
NM_001274043.1	NP_001260972.1	tout-velu, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ext1b	NP_001012369.2
Xenopus tropicalis (tropical clawed frog)	ext1	NP_001006730.1
Homo sapiens (human)	EXT1	NP_000118.2
	EXT1	XP_001096808.1
Gallus gallus (chicken)	LOC100858205	XP_003640902.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010388	XP_311558.3
Canis lupus familiaris (dog)	EXT1	XP_539145.2
Bos taurus (cattle)	EXT1	NP_001091564.1
Mus musculus (house mouse)	Ext1	NP_034292.2
Rattus norvegicus (Norway rat)	Ext1	NP_001124012.1
Caenorhabditis elegans (roundworm)	rib-1	NP_502180.1
Pan troglodytes (chimpanzee)	EXT1	XP_001141496.1
Drosophila melanogaster (fruit fly)	ttv	NP_477231.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The hEXT1 gene was able to rescue a ttv null mutant to adulthood and restore glycosaminoglycan biosynthesis.
Title: Functional conservation of the human EXT1 tumor suppressor gene and its Drosophila homolog tout velu.

A novel role for toutvelu (ttv), a regulator of heparan sulfate proteoglycan biosynthesis during germ cell migration.
Title: toutvelu, a regulator of heparan sulfate proteoglycan biosynthesis, controls guidance cues for germ-cell migration.

Results suggest that all three Drosophila EXT proteins -- tout-velu, brother of tout-velu, and sister of tout-velu -- are required for the biosynthesis of heparan sulfate proteoglycans, and for the gradient formation of morphogens.
Title: Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation.

The following ttv gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttv cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12109	NM_001274043.1 Latest version!	Drosophila melanogaster tout-velu (ttv), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09986	NM_001274042.1 Latest version!	Drosophila melanogaster tout-velu (ttv), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09986	NM_057883.3 Latest version!	Drosophila melanogaster tout-velu (ttv), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa87718	NM_001369957.1 Latest version!	Drosophila melanogaster tout-velu (ttv), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttv ( NM_001274043.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260972.1 Drosophila melanogaster tout-velu (ttv), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12109
Clone ID Related Accession (Same CDS sequence)	NM_001274043.1
Accession Version	NM_001274043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tout-velu, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGTTTC TGCTCAACTC GATGGGTGCG GAGCCGCGTC ACAACTACAC GGCGGTTATC 
TATGTGCAGA TTGGTGCCGC CCTGGGACCG AATGCAGCGC TCTACAAGCT AGTCAGGACC 
ATCACGAAGA GCCAGTTCGT GGAGCGGATC CTTGTCCTTT GGGCAGCCGA TCGGCCGTTG 
CCGTTGAAGA AACGCTGGCC CCCCACCAGC CACATACCCT TGCACGTGAT TTCCCTGGGG 
GGCAGCACGA GGAGTCAGGG AGCTGGACCC ACAAGCCAGA CGACTGAGGG ACGACCTAGC 
ATATCACAAC GATTCCTGCC CTACGATGAG ATTCAGACGG ATGCCGTTCT GTCGCTGGAT 
GAAGATGCCA TACTCAATAC GGATGAACTG GACTTTGCCT ACACGGTGTG GCGGGATTTT 
CCCGAGCGCA TCGTTGGCTA TCCGGCGAGA GCTCACTTCT GGGATGATTC CAAGAATGCC 
TGGGGTTATA CGTCCAAGTG GACAAACTAC TATTCGATTG TGCTAACTGG GGCGGCATTC 
TACCACCGCT ACTACAACTA CTTGTACACC AATTGGCTGT CGTTGCTGCT ACTTAAGACT 
GTACAGCAGT CCTCCAACTG CGAGGACATC CTAATGAACC TGCTGGTCTC GCACGTGACC 
AGAAAGCCGC CGATCAAGGT GACGCAGCGC AAGGGCTACA AGGATCGAGA GACGGGTCGT 
TCGCCCTGGA ACGATCCCGA CCACTTTATC CAGCGCCAGA GCTGCCTGAA TACCTTTGCG 
GCCGTGTTTG GCTATATGCC GCTGATACGT TCCAATTTGC GCATGGATCC CATGCTGTAT 
CGTGATCCAG TTTCCAATTT GCGCAAGAAA TACCGCCAGA TCGAGTTGGT CGGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260972.1
CDS	357..1256
Translation	MPFLLNSMGAEPRHNYTAVIYVQIGAALGPNAALYKLVRTITKSQFVERILVLWAADRPLPLKKRWPPTSHIPLHVISLGGSTRSQGAGPTSQTTEGRPSISQRFLPYDEIQTDAVLSLDEDAILNTDELDFAYTVWRDFPERIVGYPARAHFWDDSKNAWGYTSKWTNYYSIVLTGAAFYHRYYNYLYTNWLSLLLLKTVQQSSNCEDILMNLLVSHVTRKPPIKVTQRKGYKDRETGRSPWNDPDHFIQRQSCLNTFAAVFGYMPLIRSNLRMDPMLYRDPVSNLRKKYRQIELVGS

Target ORF information:

RefSeq Version	NM_001274043.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tout-velu (ttv), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274043.1

ORF Insert Sequence:

ATGCCGTTTC TGCTCAACTC GATGGGTGCG GAGCCGCGTC ACAACTACAC GGCGGTTATC 
TATGTGCAGA TTGGTGCCGC CCTGGGACCG AATGCAGCGC TCTACAAGCT AGTCAGGACC 
ATCACGAAGA GCCAGTTCGT GGAGCGGATC CTTGTCCTTT GGGCAGCCGA TCGGCCGTTG 
CCGTTGAAGA AACGCTGGCC CCCCACCAGC CACATACCCT TGCACGTGAT TTCCCTGGGG 
GGCAGCACGA GGAGTCAGGG AGCTGGACCC ACAAGCCAGA CGACTGAGGG ACGACCTAGC 
ATATCACAAC GATTCCTGCC CTACGATGAG ATTCAGACGG ATGCCGTTCT GTCGCTGGAT 
GAAGATGCCA TACTCAATAC GGATGAACTG GACTTTGCCT ACACGGTGTG GCGGGATTTT 
CCCGAGCGCA TCGTTGGCTA TCCGGCGAGA GCTCACTTCT GGGATGATTC CAAGAATGCC 
TGGGGTTATA CGTCCAAGTG GACAAACTAC TATTCGATTG TGCTAACTGG GGCGGCATTC 
TACCACCGCT ACTACAACTA CTTGTACACC AATTGGCTGT CGTTGCTGCT ACTTAAGACT 
GTACAGCAGT CCTCCAACTG CGAGGACATC CTAATGAACC TGCTGGTCTC GCACGTGACC 
AGAAAGCCGC CGATCAAGGT GACGCAGCGC AAGGGCTACA AGGATCGAGA GACGGGTCGT 
TCGCCCTGGA ACGATCCCGA CCACTTTATC CAGCGCCAGA GCTGCCTGAA TACCTTTGCG 
GCCGTGTTTG GCTATATGCC GCTGATACGT TCCAATTTGC GCATGGATCC CATGCTGTAT 
CGTGATCCAG TTTCCAATTT GCGCAAGAAA TACCGCCAGA TCGAGTTGGT CGGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttv ( NM_057883.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477231.1 Drosophila melanogaster tout-velu (ttv), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09986
Clone ID Related Accession (Same CDS sequence)	NM_057883.3 , NM_001274042.1
Accession Version	NM_057883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tout-velu, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057883.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCCA AAAAGCGTTA TATTTTGGTC TTCGTTTCCT GCGCTTTTTT GGCCTACGCC 
TATTTCGGTG GCTATCGCCT GAAAGTCTCA CCATTGAGAC CCCGTAGAGC CCAGCACGAA 
TCGGCCAAGG ATGGTGGAGT TCAACCCCAC GAGCAGTTGC CCAGCTTCCT GGGCGCCCAC 
GATATGCAGG AACTCCAACT GCTGCAGAGC AATCAATCGA AGAGTTTGGA TAGCTCCAAG 
CACCTGGTTA CCCGCAAACC CGACTGCCGC ATGGAGACCT GTTTCGATTT TACCCGCTGT 
TATGATCGCT TTTTGGTCTA TATCTATCCA CCGGAACCAC TTAACTCACT GGGCGCTGCC 
CCGCCCACCT CGGCCAACTA TCAAAAGATA CTCACTGCCA TCCAGGAATC GAGATATTAT 
ACCAGTGATC CCACGGCCGC CTGTCTCTTT GTGCTCGGTA TCGATACCCT GGATCGAGAT 
TCCCTATCCG AGGATTATGT TCGGAATGTG CCATCCAGAT TGGCAAGATT GCCGTATTGG 
AACAATGGCA GGAACCACAT TATCTTCAAT CTGTACTCGG GCACTTGGCC GGATTACGCG 
GAGAACTCAT TGGGTTTCGA TGCGGGTGAG GCCATTTTGG CCAAGGCCAG CATGGGAGTG 
CTCCAACTGC GACACGGTTT CGACGTCAGC ATCCCGCTCT TCCACAAACA ATTCCCGCTG 
AGGGCCGGCG CCACAGGAAC CGTGCAGTCC AACAATTTCC CCGCCAACAA GAAGTATCTG 
CTGGCCTTCA AAGGAAAGCG ATACGTGCAT GGCATTGGAT CGGAGACGCG CAACTCGCTC 
TTTCATTTGC ACAACGGACG CGACATGGTC CTGGTCACCA CCTGTCGCCA CGGGAAAAGT 
TGGCGGGAGC TGCAGGATAA TCGCTGCGAC GAGGACAACC GGGAATACGA CAGATACGAC 
TACGAGACCT TACTGCAAAA TTCCACTTTC TGCTTGGTGC CGCGCGGACG TCGTTTGGGT 
TCCTTTAGAT TCCTCGAAGC CTTGCAGGCT GGTTGCATTC CCGTACTATT GTCCAATGCC 
TGGGTGTTGC CGTTTGAATC GAAAATCGAT TGGAAGCAGG CCGCCATTTG GGCCGATGAG 
CGGCTCCTGC TGCAGGTACC CGACATCGTG CGCTCCATAC CGGCGGAACG CATCTTCGCC 
CTCCGCCAGC AGACGCAGGT CCTGTGGGAA CGCTACTTCG GATCCATTGA GAAAATCGTC 
TTCACAACAT TCGAGATCAT ACGCGAACGT TTGCCGGATT ATCCGGTGCG AAGCAGCCTG 
GTTTGGAACA GCTCGCCGGG CGCCCTGCTC ACGTTGCCCA CCTTTGCCGA CAGCTCAAGG 
TACATGCCGT TTCTGCTCAA CTCGATGGGT GCGGAGCCGC GTCACAACTA CACGGCGGTT 
ATCTATGTGC AGATTGGTGC CGCCCTGGGA CCGAATGCAG CGCTCTACAA GCTAGTCAGG 
ACCATCACGA AGAGCCAGTT CGTGGAGCGG ATCCTTGTCC TTTGGGCAGC CGATCGGCCG 
TTGCCGTTGA AGAAACGCTG GCCCCCCACC AGCCACATAC CCTTGCACGT GATTTCCCTG 
GGGGGCAGCA CGAGGAGTCA GGGAGCTGGA CCCACAAGCC AGACGACTGA GGGACGACCT 
AGCATATCAC AACGATTCCT GCCCTACGAT GAGATTCAGA CGGATGCCGT TCTGTCGCTG 
GATGAAGATG CCATACTCAA TACGGATGAA CTGGACTTTG CCTACACGGT GTGGCGGGAT 
TTTCCCGAGC GCATCGTTGG CTATCCGGCG AGAGCTCACT TCTGGGATGA TTCCAAGAAT 
GCCTGGGGTT ATACGTCCAA GTGGACAAAC TACTATTCGA TTGTGCTAAC TGGGGCGGCA 
TTCTACCACC GCTACTACAA CTACTTGTAC ACCAATTGGC TGTCGTTGCT GCTACTTAAG 
ACTGTACAGC AGTCCTCCAA CTGCGAGGAC ATCCTAATGA ACCTGCTGGT CTCGCACGTG 
ACCAGAAAGC CGCCGATCAA GGTGACGCAG CGCAAGGGCT ACAAGGATCG AGAGACGGGT 
CGTTCGCCCT GGAACGATCC CGACCACTTT ATCCAGCGCC AGAGCTGCCT GAATACCTTT 
GCGGCCGTGT TTGGCTATAT GCCGCTGATA CGTTCCAATT TGCGCATGGA TCCCATGCTG 
TATCGTGATC CAGTTTCCAA TTTGCGCAAG AAATACCGCC AGATCGAGTT GGTCGGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477231.1
CDS	617..2899
Translation	MQAKKRYILVFVSCAFLAYAYFGGYRLKVSPLRPRRAQHESAKDGGVQPHEQLPSFLGAHDMQELQLLQSNQSKSLDSSKHLVTRKPDCRMETCFDFTRCYDRFLVYIYPPEPLNSLGAAPPTSANYQKILTAIQESRYYTSDPTAACLFVLGIDTLDRDSLSEDYVRNVPSRLARLPYWNNGRNHIIFNLYSGTWPDYAENSLGFDAGEAILAKASMGVLQLRHGFDVSIPLFHKQFPLRAGATGTVQSNNFPANKKYLLAFKGKRYVHGIGSETRNSLFHLHNGRDMVLVTTCRHGKSWRELQDNRCDEDNREYDRYDYETLLQNSTFCLVPRGRRLGSFRFLEALQAGCIPVLLSNAWVLPFESKIDWKQAAIWADERLLLQVPDIVRSIPAERIFALRQQTQVLWERYFGSIEKIVFTTFEIIRERLPDYPVRSSLVWNSSPGALLTLPTFADSSRYMPFLLNSMGAEPRHNYTAVIYVQIGAALGPNAALYKLVRTITKSQFVERILVLWAADRPLPLKKRWPPTSHIPLHVISLGGSTRSQGAGPTSQTTEGRPSISQRFLPYDEIQTDAVLSLDEDAILNTDELDFAYTVWRDFPERIVGYPARAHFWDDSKNAWGYTSKWTNYYSIVLTGAAFYHRYYNYLYTNWLSLLLLKTVQQSSNCEDILMNLLVSHVTRKPPIKVTQRKGYKDRETGRSPWNDPDHFIQRQSCLNTFAAVFGYMPLIRSNLRMDPMLYRDPVSNLRKKYRQIELVGS

Target ORF information:

RefSeq Version	NM_057883.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tout-velu (ttv), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057883.3

ORF Insert Sequence:

ATGCAGGCCA AAAAGCGTTA TATTTTGGTC TTCGTTTCCT GCGCTTTTTT GGCCTACGCC 
TATTTCGGTG GCTATCGCCT GAAAGTCTCA CCATTGAGAC CCCGTAGAGC CCAGCACGAA 
TCGGCCAAGG ATGGTGGAGT TCAACCCCAC GAGCAGTTGC CCAGCTTCCT GGGCGCCCAC 
GATATGCAGG AACTCCAACT GCTGCAGAGC AATCAATCGA AGAGTTTGGA TAGCTCCAAG 
CACCTGGTTA CCCGCAAACC CGACTGCCGC ATGGAGACCT GTTTCGATTT TACCCGCTGT 
TATGATCGCT TTTTGGTCTA TATCTATCCA CCGGAACCAC TTAACTCACT GGGCGCTGCC 
CCGCCCACCT CGGCCAACTA TCAAAAGATA CTCACTGCCA TCCAGGAATC GAGATATTAT 
ACCAGTGATC CCACGGCCGC CTGTCTCTTT GTGCTCGGTA TCGATACCCT GGATCGAGAT 
TCCCTATCCG AGGATTATGT TCGGAATGTG CCATCCAGAT TGGCAAGATT GCCGTATTGG 
AACAATGGCA GGAACCACAT TATCTTCAAT CTGTACTCGG GCACTTGGCC GGATTACGCG 
GAGAACTCAT TGGGTTTCGA TGCGGGTGAG GCCATTTTGG CCAAGGCCAG CATGGGAGTG 
CTCCAACTGC GACACGGTTT CGACGTCAGC ATCCCGCTCT TCCACAAACA ATTCCCGCTG 
AGGGCCGGCG CCACAGGAAC CGTGCAGTCC AACAATTTCC CCGCCAACAA GAAGTATCTG 
CTGGCCTTCA AAGGAAAGCG ATACGTGCAT GGCATTGGAT CGGAGACGCG CAACTCGCTC 
TTTCATTTGC ACAACGGACG CGACATGGTC CTGGTCACCA CCTGTCGCCA CGGGAAAAGT 
TGGCGGGAGC TGCAGGATAA TCGCTGCGAC GAGGACAACC GGGAATACGA CAGATACGAC 
TACGAGACCT TACTGCAAAA TTCCACTTTC TGCTTGGTGC CGCGCGGACG TCGTTTGGGT 
TCCTTTAGAT TCCTCGAAGC CTTGCAGGCT GGTTGCATTC CCGTACTATT GTCCAATGCC 
TGGGTGTTGC CGTTTGAATC GAAAATCGAT TGGAAGCAGG CCGCCATTTG GGCCGATGAG 
CGGCTCCTGC TGCAGGTACC CGACATCGTG CGCTCCATAC CGGCGGAACG CATCTTCGCC 
CTCCGCCAGC AGACGCAGGT CCTGTGGGAA CGCTACTTCG GATCCATTGA GAAAATCGTC 
TTCACAACAT TCGAGATCAT ACGCGAACGT TTGCCGGATT ATCCGGTGCG AAGCAGCCTG 
GTTTGGAACA GCTCGCCGGG CGCCCTGCTC ACGTTGCCCA CCTTTGCCGA CAGCTCAAGG 
TACATGCCGT TTCTGCTCAA CTCGATGGGT GCGGAGCCGC GTCACAACTA CACGGCGGTT 
ATCTATGTGC AGATTGGTGC CGCCCTGGGA CCGAATGCAG CGCTCTACAA GCTAGTCAGG 
ACCATCACGA AGAGCCAGTT CGTGGAGCGG ATCCTTGTCC TTTGGGCAGC CGATCGGCCG 
TTGCCGTTGA AGAAACGCTG GCCCCCCACC AGCCACATAC CCTTGCACGT GATTTCCCTG 
GGGGGCAGCA CGAGGAGTCA GGGAGCTGGA CCCACAAGCC AGACGACTGA GGGACGACCT 
AGCATATCAC AACGATTCCT GCCCTACGAT GAGATTCAGA CGGATGCCGT TCTGTCGCTG 
GATGAAGATG CCATACTCAA TACGGATGAA CTGGACTTTG CCTACACGGT GTGGCGGGAT 
TTTCCCGAGC GCATCGTTGG CTATCCGGCG AGAGCTCACT TCTGGGATGA TTCCAAGAAT 
GCCTGGGGTT ATACGTCCAA GTGGACAAAC TACTATTCGA TTGTGCTAAC TGGGGCGGCA 
TTCTACCACC GCTACTACAA CTACTTGTAC ACCAATTGGC TGTCGTTGCT GCTACTTAAG 
ACTGTACAGC AGTCCTCCAA CTGCGAGGAC ATCCTAATGA ACCTGCTGGT CTCGCACGTG 
ACCAGAAAGC CGCCGATCAA GGTGACGCAG CGCAAGGGCT ACAAGGATCG AGAGACGGGT 
CGTTCGCCCT GGAACGATCC CGACCACTTT ATCCAGCGCC AGAGCTGCCT GAATACCTTT 
GCGGCCGTGT TTGGCTATAT GCCGCTGATA CGTTCCAATT TGCGCATGGA TCCCATGCTG 
TATCGTGATC CAGTTTCCAA TTTGCGCAAG AAATACCGCC AGATCGAGTT GGTCGGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttv ( NM_001274042.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260971.1 Drosophila melanogaster tout-velu (ttv), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09986
Clone ID Related Accession (Same CDS sequence)	NM_057883.3 , NM_001274042.1
Accession Version	NM_001274042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tout-velu, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCCA AAAAGCGTTA TATTTTGGTC TTCGTTTCCT GCGCTTTTTT GGCCTACGCC 
TATTTCGGTG GCTATCGCCT GAAAGTCTCA CCATTGAGAC CCCGTAGAGC CCAGCACGAA 
TCGGCCAAGG ATGGTGGAGT TCAACCCCAC GAGCAGTTGC CCAGCTTCCT GGGCGCCCAC 
GATATGCAGG AACTCCAACT GCTGCAGAGC AATCAATCGA AGAGTTTGGA TAGCTCCAAG 
CACCTGGTTA CCCGCAAACC CGACTGCCGC ATGGAGACCT GTTTCGATTT TACCCGCTGT 
TATGATCGCT TTTTGGTCTA TATCTATCCA CCGGAACCAC TTAACTCACT GGGCGCTGCC 
CCGCCCACCT CGGCCAACTA TCAAAAGATA CTCACTGCCA TCCAGGAATC GAGATATTAT 
ACCAGTGATC CCACGGCCGC CTGTCTCTTT GTGCTCGGTA TCGATACCCT GGATCGAGAT 
TCCCTATCCG AGGATTATGT TCGGAATGTG CCATCCAGAT TGGCAAGATT GCCGTATTGG 
AACAATGGCA GGAACCACAT TATCTTCAAT CTGTACTCGG GCACTTGGCC GGATTACGCG 
GAGAACTCAT TGGGTTTCGA TGCGGGTGAG GCCATTTTGG CCAAGGCCAG CATGGGAGTG 
CTCCAACTGC GACACGGTTT CGACGTCAGC ATCCCGCTCT TCCACAAACA ATTCCCGCTG 
AGGGCCGGCG CCACAGGAAC CGTGCAGTCC AACAATTTCC CCGCCAACAA GAAGTATCTG 
CTGGCCTTCA AAGGAAAGCG ATACGTGCAT GGCATTGGAT CGGAGACGCG CAACTCGCTC 
TTTCATTTGC ACAACGGACG CGACATGGTC CTGGTCACCA CCTGTCGCCA CGGGAAAAGT 
TGGCGGGAGC TGCAGGATAA TCGCTGCGAC GAGGACAACC GGGAATACGA CAGATACGAC 
TACGAGACCT TACTGCAAAA TTCCACTTTC TGCTTGGTGC CGCGCGGACG TCGTTTGGGT 
TCCTTTAGAT TCCTCGAAGC CTTGCAGGCT GGTTGCATTC CCGTACTATT GTCCAATGCC 
TGGGTGTTGC CGTTTGAATC GAAAATCGAT TGGAAGCAGG CCGCCATTTG GGCCGATGAG 
CGGCTCCTGC TGCAGGTACC CGACATCGTG CGCTCCATAC CGGCGGAACG CATCTTCGCC 
CTCCGCCAGC AGACGCAGGT CCTGTGGGAA CGCTACTTCG GATCCATTGA GAAAATCGTC 
TTCACAACAT TCGAGATCAT ACGCGAACGT TTGCCGGATT ATCCGGTGCG AAGCAGCCTG 
GTTTGGAACA GCTCGCCGGG CGCCCTGCTC ACGTTGCCCA CCTTTGCCGA CAGCTCAAGG 
TACATGCCGT TTCTGCTCAA CTCGATGGGT GCGGAGCCGC GTCACAACTA CACGGCGGTT 
ATCTATGTGC AGATTGGTGC CGCCCTGGGA CCGAATGCAG CGCTCTACAA GCTAGTCAGG 
ACCATCACGA AGAGCCAGTT CGTGGAGCGG ATCCTTGTCC TTTGGGCAGC CGATCGGCCG 
TTGCCGTTGA AGAAACGCTG GCCCCCCACC AGCCACATAC CCTTGCACGT GATTTCCCTG 
GGGGGCAGCA CGAGGAGTCA GGGAGCTGGA CCCACAAGCC AGACGACTGA GGGACGACCT 
AGCATATCAC AACGATTCCT GCCCTACGAT GAGATTCAGA CGGATGCCGT TCTGTCGCTG 
GATGAAGATG CCATACTCAA TACGGATGAA CTGGACTTTG CCTACACGGT GTGGCGGGAT 
TTTCCCGAGC GCATCGTTGG CTATCCGGCG AGAGCTCACT TCTGGGATGA TTCCAAGAAT 
GCCTGGGGTT ATACGTCCAA GTGGACAAAC TACTATTCGA TTGTGCTAAC TGGGGCGGCA 
TTCTACCACC GCTACTACAA CTACTTGTAC ACCAATTGGC TGTCGTTGCT GCTACTTAAG 
ACTGTACAGC AGTCCTCCAA CTGCGAGGAC ATCCTAATGA ACCTGCTGGT CTCGCACGTG 
ACCAGAAAGC CGCCGATCAA GGTGACGCAG CGCAAGGGCT ACAAGGATCG AGAGACGGGT 
CGTTCGCCCT GGAACGATCC CGACCACTTT ATCCAGCGCC AGAGCTGCCT GAATACCTTT 
GCGGCCGTGT TTGGCTATAT GCCGCTGATA CGTTCCAATT TGCGCATGGA TCCCATGCTG 
TATCGTGATC CAGTTTCCAA TTTGCGCAAG AAATACCGCC AGATCGAGTT GGTCGGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260971.1
CDS	926..3208
Translation	MQAKKRYILVFVSCAFLAYAYFGGYRLKVSPLRPRRAQHESAKDGGVQPHEQLPSFLGAHDMQELQLLQSNQSKSLDSSKHLVTRKPDCRMETCFDFTRCYDRFLVYIYPPEPLNSLGAAPPTSANYQKILTAIQESRYYTSDPTAACLFVLGIDTLDRDSLSEDYVRNVPSRLARLPYWNNGRNHIIFNLYSGTWPDYAENSLGFDAGEAILAKASMGVLQLRHGFDVSIPLFHKQFPLRAGATGTVQSNNFPANKKYLLAFKGKRYVHGIGSETRNSLFHLHNGRDMVLVTTCRHGKSWRELQDNRCDEDNREYDRYDYETLLQNSTFCLVPRGRRLGSFRFLEALQAGCIPVLLSNAWVLPFESKIDWKQAAIWADERLLLQVPDIVRSIPAERIFALRQQTQVLWERYFGSIEKIVFTTFEIIRERLPDYPVRSSLVWNSSPGALLTLPTFADSSRYMPFLLNSMGAEPRHNYTAVIYVQIGAALGPNAALYKLVRTITKSQFVERILVLWAADRPLPLKKRWPPTSHIPLHVISLGGSTRSQGAGPTSQTTEGRPSISQRFLPYDEIQTDAVLSLDEDAILNTDELDFAYTVWRDFPERIVGYPARAHFWDDSKNAWGYTSKWTNYYSIVLTGAAFYHRYYNYLYTNWLSLLLLKTVQQSSNCEDILMNLLVSHVTRKPPIKVTQRKGYKDRETGRSPWNDPDHFIQRQSCLNTFAAVFGYMPLIRSNLRMDPMLYRDPVSNLRKKYRQIELVGS

Target ORF information:

RefSeq Version	NM_001274042.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tout-velu (ttv), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274042.1

ORF Insert Sequence:

ATGCAGGCCA AAAAGCGTTA TATTTTGGTC TTCGTTTCCT GCGCTTTTTT GGCCTACGCC 
TATTTCGGTG GCTATCGCCT GAAAGTCTCA CCATTGAGAC CCCGTAGAGC CCAGCACGAA 
TCGGCCAAGG ATGGTGGAGT TCAACCCCAC GAGCAGTTGC CCAGCTTCCT GGGCGCCCAC 
GATATGCAGG AACTCCAACT GCTGCAGAGC AATCAATCGA AGAGTTTGGA TAGCTCCAAG 
CACCTGGTTA CCCGCAAACC CGACTGCCGC ATGGAGACCT GTTTCGATTT TACCCGCTGT 
TATGATCGCT TTTTGGTCTA TATCTATCCA CCGGAACCAC TTAACTCACT GGGCGCTGCC 
CCGCCCACCT CGGCCAACTA TCAAAAGATA CTCACTGCCA TCCAGGAATC GAGATATTAT 
ACCAGTGATC CCACGGCCGC CTGTCTCTTT GTGCTCGGTA TCGATACCCT GGATCGAGAT 
TCCCTATCCG AGGATTATGT TCGGAATGTG CCATCCAGAT TGGCAAGATT GCCGTATTGG 
AACAATGGCA GGAACCACAT TATCTTCAAT CTGTACTCGG GCACTTGGCC GGATTACGCG 
GAGAACTCAT TGGGTTTCGA TGCGGGTGAG GCCATTTTGG CCAAGGCCAG CATGGGAGTG 
CTCCAACTGC GACACGGTTT CGACGTCAGC ATCCCGCTCT TCCACAAACA ATTCCCGCTG 
AGGGCCGGCG CCACAGGAAC CGTGCAGTCC AACAATTTCC CCGCCAACAA GAAGTATCTG 
CTGGCCTTCA AAGGAAAGCG ATACGTGCAT GGCATTGGAT CGGAGACGCG CAACTCGCTC 
TTTCATTTGC ACAACGGACG CGACATGGTC CTGGTCACCA CCTGTCGCCA CGGGAAAAGT 
TGGCGGGAGC TGCAGGATAA TCGCTGCGAC GAGGACAACC GGGAATACGA CAGATACGAC 
TACGAGACCT TACTGCAAAA TTCCACTTTC TGCTTGGTGC CGCGCGGACG TCGTTTGGGT 
TCCTTTAGAT TCCTCGAAGC CTTGCAGGCT GGTTGCATTC CCGTACTATT GTCCAATGCC 
TGGGTGTTGC CGTTTGAATC GAAAATCGAT TGGAAGCAGG CCGCCATTTG GGCCGATGAG 
CGGCTCCTGC TGCAGGTACC CGACATCGTG CGCTCCATAC CGGCGGAACG CATCTTCGCC 
CTCCGCCAGC AGACGCAGGT CCTGTGGGAA CGCTACTTCG GATCCATTGA GAAAATCGTC 
TTCACAACAT TCGAGATCAT ACGCGAACGT TTGCCGGATT ATCCGGTGCG AAGCAGCCTG 
GTTTGGAACA GCTCGCCGGG CGCCCTGCTC ACGTTGCCCA CCTTTGCCGA CAGCTCAAGG 
TACATGCCGT TTCTGCTCAA CTCGATGGGT GCGGAGCCGC GTCACAACTA CACGGCGGTT 
ATCTATGTGC AGATTGGTGC CGCCCTGGGA CCGAATGCAG CGCTCTACAA GCTAGTCAGG 
ACCATCACGA AGAGCCAGTT CGTGGAGCGG ATCCTTGTCC TTTGGGCAGC CGATCGGCCG 
TTGCCGTTGA AGAAACGCTG GCCCCCCACC AGCCACATAC CCTTGCACGT GATTTCCCTG 
GGGGGCAGCA CGAGGAGTCA GGGAGCTGGA CCCACAAGCC AGACGACTGA GGGACGACCT 
AGCATATCAC AACGATTCCT GCCCTACGAT GAGATTCAGA CGGATGCCGT TCTGTCGCTG 
GATGAAGATG CCATACTCAA TACGGATGAA CTGGACTTTG CCTACACGGT GTGGCGGGAT 
TTTCCCGAGC GCATCGTTGG CTATCCGGCG AGAGCTCACT TCTGGGATGA TTCCAAGAAT 
GCCTGGGGTT ATACGTCCAA GTGGACAAAC TACTATTCGA TTGTGCTAAC TGGGGCGGCA 
TTCTACCACC GCTACTACAA CTACTTGTAC ACCAATTGGC TGTCGTTGCT GCTACTTAAG 
ACTGTACAGC AGTCCTCCAA CTGCGAGGAC ATCCTAATGA ACCTGCTGGT CTCGCACGTG 
ACCAGAAAGC CGCCGATCAA GGTGACGCAG CGCAAGGGCT ACAAGGATCG AGAGACGGGT 
CGTTCGCCCT GGAACGATCC CGACCACTTT ATCCAGCGCC AGAGCTGCCT GAATACCTTT 
GCGGCCGTGT TTGGCTATAT GCCGCTGATA CGTTCCAATT TGCGCATGGA TCCCATGCTG 
TATCGTGATC CAGTTTCCAA TTTGCGCAAG AAATACCGCC AGATCGAGTT GGTCGGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttv ( NM_001369957.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356963.1 Drosophila melanogaster tout-velu (ttv), transcript variant D, mRNA.

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CloneID	OFa87718
Clone ID Related Accession (Same CDS sequence)	NM_001369957.1
Accession Version	NM_001369957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tout-velu, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCCA AAAAGCGTTA TATTTTGGTC TTCGTTTCCT GCGCTTTTTT GGCCTACGCC 
TATTTCGGTG GCTATCGCCT GAAAGTCTCA CCATTGAGAC CCCGTAGAGC CCAGCACGAA 
TCGGCCAAGG ATGGTGGAGT TCAACCCCAC GAGCAGTTGC CCAGCTTCCT GGGCGCCCAC 
GATATGCAGG AACTCCAACT GCTGCAGAGC AATCAATCGA AGAGTTTGGA TAGCTCCAAG 
CACCTGGTTA CCCGCAAACC CGACTGCCGC ATGGAGACCT GTTTCGATTT TACCCGCTGT 
TATGATCGCT TTTTGGTCTA TATCTATCCA CCGGAACCAC TTAACTCACT GGGCGCTGCC 
CCGCCCACCT CGGCCAACTA TCAAAAGATA CTCACTGCCA TCCAGGAATC GAGATATTAT 
ACCAGTGATC CCACGGCCGC CTGTCTCTTT GTGCTCGGTA TCGATACCCT GGATCGAGAT 
TCCCTATCCG AGGATTATGT TCGGAATGTG CCATCCAGAT TGGCAAGATT GCCGTATTGG 
AACAATGGCA GGAACCACAT TATCTTCAAT CTGTACTCGG GCACTTGGCC GGATTACGCG 
GAGAACTCAT TGGGTTTCGA TGCGGGTGAG GCCATTTTGG CCAAGGCCAG CATGGGAGTG 
CTCCAACTGC GACACGGTTT CGACGTCAGC ATCCCGCTCT TCCACAAACA ATTCCCGCTG 
AGGGCCGGCG CCACAGGAAC CGTGCAGTCC AACAATTTCC CCGCCAACAA GAAGTATCTG 
CTGGCCTTCA AAGGAAAGCG ATACGTGCAT GGCATTGGAT CGGAGACGCG CAACTCGCTC 
TTTCATTTGC ACAACGGACG CGACATGGTC CTGGTCACCA CCTGTCGCCA CGGGAAAAGT 
TGGCGGGAGC TGCAGGATAA TCGCTGCGAC GAGGACAACC GGGAATACGA CAGATACGAC 
TACGAGACCT TACTGCAAAA TTCCACTTTC TGCTTGGTGC CGCGCGGACG TCGTTTGGGT 
TCCTTTAGAT TCCTCGAAGC CTTGCAGGCT GGTTGCATTC CCGTACTATT GTCCAATGCC 
TGGGTGTTGC CGTTTGAATC GAAAATCGAT TGGAAGCAGG CCGCCATTTG GGCCGATGAG 
CGGCTCCTGC TGCAGGTACC CGACATCGTG CGCTCCATAC CGGCGGAACG CATCTTCGCC 
CTCCGCCAGC AGACGCAGGT CCTGTGGGAA CGCTACTTCG GATCCATTGA GAAAATCGTC 
TTCACAACAT TCGAGATCAT ACGCGAACGT TTGCCGGATT ATCCGGTGCG AAGCAGCCTG 
GTTTGGAACA GCTCGCCGGG CGCCCTGCTC ACGTTGCCCA CCTTTGCCGA CAGCTCAAGG 
TACATGCCGT TTCTGCTCAA CTCGATGGGT GCGGAGCCGC GTCACAACTA CACGGCGGTT 
ATCTATGTGC AGATTGGTGC CGCCCTGGGA CCGAATGCAG CGCTCTACAA GCTAGTCAGG 
ACCATCACGA AGAGCCAGTT CGTGGAGCGG ATCCTTGTCC TTTGGGCAGC CGATCGGCCG 
TTGCCGTTGA AGAAACGCTG GCCCCCCACC AGCCACATAC CCTTGCACGT GATTTCCCTG 
GGGGGCAGCA CGAGGAGTCA GGGAGCTGGA CCCACAAGCC AGACGACTGA GGGACGACCT 
AGCATATCAC AACGATTCCT GCCCTACGAT GAGATTCAGA CGGATGCCGT TCTGTCGCTG 
GATGAAGATG CCATACTCAA TACGGATGAA CTGGACTTTG CCTACACGGT GTGGCGGGAT 
TTTCCCGAGC GCATCGTTGG CTATCCGGCG AGAGCTCACT TCTGGGATGA TTCCAAGAAT 
GCCTGGGGTT ATACGTCCAA GTGGACAAAC TACTATTCGA TTGTGCTAAC TGGGGCGGCA 
TTCTACCACC GCTACTACAA CTACTTGTAC ACCAATTGGC TGTCGTTGCT GCTACTTAAG 
ACTGTACAGC AGTCCTCCAA CTGCGAGGAC ATCCTAATGA ACCTGCTGGT CTCGCACGTG 
ACCAGAAAGC CGCCGATCAA GGTGACGCAG CGCAAGGGCT ACAAGGATCG AGAGACGGGT 
CGTTCGCCCT GGAACGATCC CGACCACTTT ATCCAGCGCC AGAGCTGCCT GAATACCTTT 
GCGGCCGTGT TTGGCTATAT GCCGCTGATA CGTTCCAATT TGCGCATGGA TCCCATGCTG 
TATCGTGATC CAGTTTCCAA TTTGCGCAAG AAATACCGCC AGATCGAGTT GGTCGGCAGC 
TAGCGATATG TGCATCCTGG CAATAGCTAC AAGCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356963.1
CDS	617..2935
Translation	MQAKKRYILVFVSCAFLAYAYFGGYRLKVSPLRPRRAQHESAKDGGVQPHEQLPSFLGAHDMQELQLLQSNQSKSLDSSKHLVTRKPDCRMETCFDFTRCYDRFLVYIYPPEPLNSLGAAPPTSANYQKILTAIQESRYYTSDPTAACLFVLGIDTLDRDSLSEDYVRNVPSRLARLPYWNNGRNHIIFNLYSGTWPDYAENSLGFDAGEAILAKASMGVLQLRHGFDVSIPLFHKQFPLRAGATGTVQSNNFPANKKYLLAFKGKRYVHGIGSETRNSLFHLHNGRDMVLVTTCRHGKSWRELQDNRCDEDNREYDRYDYETLLQNSTFCLVPRGRRLGSFRFLEALQAGCIPVLLSNAWVLPFESKIDWKQAAIWADERLLLQVPDIVRSIPAERIFALRQQTQVLWERYFGSIEKIVFTTFEIIRERLPDYPVRSSLVWNSSPGALLTLPTFADSSRYMPFLLNSMGAEPRHNYTAVIYVQIGAALGPNAALYKLVRTITKSQFVERILVLWAADRPLPLKKRWPPTSHIPLHVISLGGSTRSQGAGPTSQTTEGRPSISQRFLPYDEIQTDAVLSLDEDAILNTDELDFAYTVWRDFPERIVGYPARAHFWDDSKNAWGYTSKWTNYYSIVLTGAAFYHRYYNYLYTNWLSLLLLKTVQQSSNCEDILMNLLVSHVTRKPPIKVTQRKGYKDRETGRSPWNDPDHFIQRQSCLNTFAAVFGYMPLIRSNLRMDPMLYRDPVSNLRKKYRQIELVGS*RYVHPGNSYKR

Target ORF information:

RefSeq Version	NM_001369957.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tout-velu (ttv), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369957.1

ORF Insert Sequence:

ATGCAGGCCA AAAAGCGTTA TATTTTGGTC TTCGTTTCCT GCGCTTTTTT GGCCTACGCC 
TATTTCGGTG GCTATCGCCT GAAAGTCTCA CCATTGAGAC CCCGTAGAGC CCAGCACGAA 
TCGGCCAAGG ATGGTGGAGT TCAACCCCAC GAGCAGTTGC CCAGCTTCCT GGGCGCCCAC 
GATATGCAGG AACTCCAACT GCTGCAGAGC AATCAATCGA AGAGTTTGGA TAGCTCCAAG 
CACCTGGTTA CCCGCAAACC CGACTGCCGC ATGGAGACCT GTTTCGATTT TACCCGCTGT 
TATGATCGCT TTTTGGTCTA TATCTATCCA CCGGAACCAC TTAACTCACT GGGCGCTGCC 
CCGCCCACCT CGGCCAACTA TCAAAAGATA CTCACTGCCA TCCAGGAATC GAGATATTAT 
ACCAGTGATC CCACGGCCGC CTGTCTCTTT GTGCTCGGTA TCGATACCCT GGATCGAGAT 
TCCCTATCCG AGGATTATGT TCGGAATGTG CCATCCAGAT TGGCAAGATT GCCGTATTGG 
AACAATGGCA GGAACCACAT TATCTTCAAT CTGTACTCGG GCACTTGGCC GGATTACGCG 
GAGAACTCAT TGGGTTTCGA TGCGGGTGAG GCCATTTTGG CCAAGGCCAG CATGGGAGTG 
CTCCAACTGC GACACGGTTT CGACGTCAGC ATCCCGCTCT TCCACAAACA ATTCCCGCTG 
AGGGCCGGCG CCACAGGAAC CGTGCAGTCC AACAATTTCC CCGCCAACAA GAAGTATCTG 
CTGGCCTTCA AAGGAAAGCG ATACGTGCAT GGCATTGGAT CGGAGACGCG CAACTCGCTC 
TTTCATTTGC ACAACGGACG CGACATGGTC CTGGTCACCA CCTGTCGCCA CGGGAAAAGT 
TGGCGGGAGC TGCAGGATAA TCGCTGCGAC GAGGACAACC GGGAATACGA CAGATACGAC 
TACGAGACCT TACTGCAAAA TTCCACTTTC TGCTTGGTGC CGCGCGGACG TCGTTTGGGT 
TCCTTTAGAT TCCTCGAAGC CTTGCAGGCT GGTTGCATTC CCGTACTATT GTCCAATGCC 
TGGGTGTTGC CGTTTGAATC GAAAATCGAT TGGAAGCAGG CCGCCATTTG GGCCGATGAG 
CGGCTCCTGC TGCAGGTACC CGACATCGTG CGCTCCATAC CGGCGGAACG CATCTTCGCC 
CTCCGCCAGC AGACGCAGGT CCTGTGGGAA CGCTACTTCG GATCCATTGA GAAAATCGTC 
TTCACAACAT TCGAGATCAT ACGCGAACGT TTGCCGGATT ATCCGGTGCG AAGCAGCCTG 
GTTTGGAACA GCTCGCCGGG CGCCCTGCTC ACGTTGCCCA CCTTTGCCGA CAGCTCAAGG 
TACATGCCGT TTCTGCTCAA CTCGATGGGT GCGGAGCCGC GTCACAACTA CACGGCGGTT 
ATCTATGTGC AGATTGGTGC CGCCCTGGGA CCGAATGCAG CGCTCTACAA GCTAGTCAGG 
ACCATCACGA AGAGCCAGTT CGTGGAGCGG ATCCTTGTCC TTTGGGCAGC CGATCGGCCG 
TTGCCGTTGA AGAAACGCTG GCCCCCCACC AGCCACATAC CCTTGCACGT GATTTCCCTG 
GGGGGCAGCA CGAGGAGTCA GGGAGCTGGA CCCACAAGCC AGACGACTGA GGGACGACCT 
AGCATATCAC AACGATTCCT GCCCTACGAT GAGATTCAGA CGGATGCCGT TCTGTCGCTG 
GATGAAGATG CCATACTCAA TACGGATGAA CTGGACTTTG CCTACACGGT GTGGCGGGAT 
TTTCCCGAGC GCATCGTTGG CTATCCGGCG AGAGCTCACT TCTGGGATGA TTCCAAGAAT 
GCCTGGGGTT ATACGTCCAA GTGGACAAAC TACTATTCGA TTGTGCTAAC TGGGGCGGCA 
TTCTACCACC GCTACTACAA CTACTTGTAC ACCAATTGGC TGTCGTTGCT GCTACTTAAG 
ACTGTACAGC AGTCCTCCAA CTGCGAGGAC ATCCTAATGA ACCTGCTGGT CTCGCACGTG 
ACCAGAAAGC CGCCGATCAA GGTGACGCAG CGCAAGGGCT ACAAGGATCG AGAGACGGGT 
CGTTCGCCCT GGAACGATCC CGACCACTTT ATCCAGCGCC AGAGCTGCCT GAATACCTTT 
GCGGCCGTGT TTGGCTATAT GCCGCTGATA CGTTCCAATT TGCGCATGGA TCCCATGCTG 
TATCGTGATC CAGTTTCCAA TTTGCGCAAG AAATACCGCC AGATCGAGTT GGTCGGCAGC 
TAGCGATATG TGCATCCTGG CAATAGCTAC AAGCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ttv cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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