Asx cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asx
Entrez Gene ID	36612
Full Name	Additional sex combs
Synonyms	ASX,CG8787,Dmel\CG8787,FBgn0261823,xen
General protein information	Preferred Names Additional sex combs Names CG8787 gene product from transcript CG8787-RD Asx-PA Asx-PB Asx-PC Asx-PD CG8787-PA CG8787-PB CG8787-PC CG8787-PD additional Sex combs additional sex comb additional sex combs additionnal sex comb xenicid
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51A5-51C1\|2-72 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014527.2	NP_001014527.1	additional sex combs, isoform B
NM_001274041.1	NP_001260970.1	additional sex combs, isoform D
NM_001274040.1	NP_001260969.1	additional sex combs, isoform C
NM_166057.2	NP_725398.1	additional sex combs, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Asx modulates the ratio of H3K4me3 to H3K27me3 during transcription of hsp70 by direct interaction with enhancer of zeste and trithorax.
Title: Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70.

A mutation that disrupts the catalytic activity of Calypso, or absence of the ASX subunit abolishes H2A deubiquitination in vitro and HOX gene repression in vivo
Title: Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB.

Expression of the betaPS integrin or the patterning protein Engrailed are not affected in developing wing imaginal discs in xenicid mutants.
Title: Wing defects in Drosophila xenicid mutant clones are caused by C-terminal deletion of additional sex combs (Asx).

The following Asx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09983	NM_166057.2 Latest version!	Drosophila melanogaster additional sex combs, transcript variant A (Asx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa09984	NM_001014527.2 Latest version!	Drosophila melanogaster additional sex combs, transcript variant B (Asx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa12107	NM_001274041.1 Latest version!	Drosophila melanogaster additional sex combs, transcript variant D (Asx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa12108	NM_001274040.1 Latest version!	Drosophila melanogaster additional sex combs, transcript variant C (Asx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asx ( NM_166057.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725398.1 Drosophila melanogaster additional sex combs, transcript variant A (Asx), mRNA.

Price & Availability↑

CloneID	OFa09983
Clone ID Related Accession (Same CDS sequence)	NM_166057.2
Accession Version	NM_166057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	additional sex combs, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166057.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCCTATT TTCAAGAACA TCAATCCACT ATCAATCACA ACTTCTTCAC TTCATCTTCA 
TCGTCCAACA CGGCGACAAC TGCAGCTAAT AAACTGGAAG AGCATAGTGA TAAGCCGGAG 
GACTCACCGC TCCCCATTGC AAGCTCAATT TCTGGGTCGA CGCCGGCCAG TTCGATTACA 
TCGACCAGCT GCACATCGTC CTCATCTTCC TCCGCCTCAA TGTCATCTTC GTGCTCCAGT 
AGCAATTCCG GCTCGACGAC GACCGCGCCC ACAACTTCAT CGTCGGCCGG AGCACCAACG 
GCTCCACTGA CGCTGGCAGC AGCAGCTGAA ACTACGTTGG CAAATGTCCA AGCCATGCTT 
TCAACTGTGG CCAAGTTACA GCAGCAGCAA CAGGAGTTAC CCGTGGAGTT GAACTCCAAT 
GAAATGTACC AGCATGTGCA GCACGATTGG AACTTTGGCG ACATTAAGTT AAGCAGTTCG 
CAGTCAAGCG GAGATCAGCA GCGTAATCTA AGCCATGAAG CAATCGATCT GATGGATGTA 
GTGCAAGATG CCGACGTCAT CGACGACATT ATGCACAATG ATGTGTGCCA CGATGTTCTC 
GGTGACGAAG ATGAGGGTGA TCAAGAGGAG GATGAAGACG ATGAAGTGGT AGAATGTATG 
ACGGAGGAAC AGCAGTTAAT TGATGAAGAC AGCGAAGCTG TCCGTGAAAT AGTGGACAAA 
CTGCAGCAGC ATCAGCAGCA ACAAAATCAG CAGCAGCATC ATCAACAGTT GCATATCCAG 
GATGTCGTCC AATTGGCTCA GCATTCGTTT ATGCCGCAGG CCCATAGTGA ATTTGGAAAT 
GATATCGGCC AGGAAATGCT CTGTGATGCT GTACCAATGT CTGCTGCCGA AATGGAAGTG 
TCCAGCACGG TGATAACCAA TAGTAGCAAT AGCAACGACA GCAGCAACAA CATAAGCCTA 
TGCAGCAGCA CCAACAGTCT TACCATTAAT CAAATGCCGC ACCAAGCATC GCAACAGCCG 
CAACAGAACG CTCAGTCCAA TGCACAGCAG CAAAGACAGA TATTGGTGGA TTCCAATGGT 
CAGATAATTG GCAACTTTTT GCTTCAGCAG CAACGCCAAC AACAGCAACA GCAACTACTA 
CAACAGTTCA CGCTGCAGGC GGCGGCAGCA CAGCAGCAGC AGCAACAACA ACAACAGCAC 
CAGCAACAGC AGCAGCAGCA ACAGCAAGCA ACAAGTAGCA ATTCCTTGGG CAAGACATTA 
CCAGTGGCTC TCCGCAACGG AACCCAACAG TTCTTGTCTC CTAACCTGAT CGCACAGCAA 
CATCAGCAGC AGCAGCAGCA ACAACTGGAG CAACATCAAC AGCAGGCTAC GGCACAACAA 
AAGCATCAGC AGATTCAGCA GTTTGCCTTG CAGCAGGCGC AACTACACCA GCGACAACTT 
CTCGCCCAGG CCGCCAACAA CAATCTGCTA CAACAGCAGC AACAACAGCA GCAAAATGTT 
GCACTACCAA CAACACAGGC TAAATTCATA GCAAAGCCAC TAAATATCAT CTCAATGACG 
CGTCCTGCCA ATGCATCACC TACCACAGCA GCAACGACGG CAAATACCGC ATCTATTCCG 
TCCGCCTATG CCAATGTTGT TGCTGTGACG GGCGCGCAAC AGCAGCAATC TCCGCCAGTA 
CCTGCCCCTC AGCAGCAGAC AGTCCAACAA CAACAGTTGG CAAATCACAA CAGTAATATG 
CAGCAGTTAC CCAACGTGCT GACTATGAAA ACTCTGCCAC CATCGGGAGT GCCAACCACC 
ATTGCCCAGC AAAGATTGCA GCCAAAAATG CCAACGGGAA AAGGGCGCAA AGCAACCAGC 
AATAGGTTAC CACCGGGTGC AGTAAATCTG GAGCGTAGCT ATCAAATCTG CCAGGCTGTT 
ATCCAGAATA GTCCAAACCG CGAGAATCTC AAGGCTCAGT TGCGACCGCC TGCGGCGATT 
CTCAACCAAC ATCAGCCGAC GACAACCACT GCGCCAGCAC CTATAAACCC TGTGACCTTG 
AATGTATCAA CGGTGGCTGC CACACCCATG TCCAACATAA CGACGGCCAC GGGTAGCATG 
GCAGCTGCAG TCGCAGCAGC ACCCCCACAA AATGTATTAA AGCAAGAGGA GCTGTTGGTC 
AGTGGAGCAG TTGGTGCTGG AGCTCTGCCC GCTGGATTGC CGCCGAATGT CATGGGTGTC 
GGACGTCCGG GAGTATACAA GGTTATTGGA CCGCGTATGA GTGGTTTCCC AAGAAAGAAA 
TATGTCCAAC GGAAGCCGTC ACCCACAACG CTCATCCGGC ATGTGTTTAG TCCCGGACCT 
GGAGGAGCTA CAGCCACCGC TCAGCAATTG CAAATGCTTC AGCAGCATCA CCAGTCGACG 
ACTTCGCCAG TGCCTGTACA AAATCCTCAG CAGCCAGCAC CGGAGCAGTT GATACACCAA 
AATGGCAATG GACAATATGT TCTCGTTCAT CGGGCGAATG TGGGTGCAGC TGACAATCAA 
GCGCCTAGAG CCTCCAGTGC CCCGCCAATG CATCAAAACC AGTTCGTTAC TGTTCAGAAT 
CCGCTGCACA GCATAAACGG CATTCCTATG GGTGGACGCG GGCGTCCAGC ATCGGTGGAC 
ACAACGGCTG GCAGCGGCAA CGTTATAGCA CCACCAATTT CCGCCACAGA TGCGTTGCAT 
CATCATCACC ACGAAATGCA GCAGCAGCAG CAACATCAGC AGCCGCAGCC GCTGGGCAAT 
GTAGGCGCAG CCGCGAATAT TGTGCGGCGC AATATCGCTG CAGGACCCAA CATCGCCTAC 
ATTGACGGCA GCAATACCAA CTCATCGGCC GTCGCGCTTA TGGAAGCTGG CAACAACTAC 
ATAGTGACGA CCAACGCGTC ACCAACTGCA GCACCTTCGC CGATCAACCA GCAGCCCCAA 
TCGCAACCAA CTGGGACTCA GCATCAGCAT CCATTGCTGC AATTGCATCA AACCGGGGAG 
AACACTCCGC CGGGCAATGA AGCTACGGCC ACGGCGAACA ACTGCGCCTG CTCACTCAAT 
GCGATGGTGA TCTGCCAACA GTGCGGAGCC TTTTGCCACG ACGACTGCAT CGGTGCGGCC 
AAGCTGTGTG TCGCCTGTGT GATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725398.1
CDS	492..5501
Translation	MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRRHLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAISPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLYQYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLKHFEPFWGEKNSRGKDKDKLESDCKNQKLSASIKSEPKPPATSQQKPLQQATCDNETELKFDLSTKCETTSAKTTVAVAVADKSSTFPPTGSQNNVLNEQQRRVLKRPSSSPSQRKQAPTTIATINLDDDLDELPSTSKDSKQPKMDEIVPNASGNVVAAPMVDVVDHSAVEMKIKDEQQHQRQHQPLINSTCDKIEPSECSKEMIVAMKQVDSKEDVDSIASAAAMPAIAAVTPHTPKPEALAPNPDVANQFVSYLQNVELAAETKAPLDNSNEADITTGTNSHDFVFSDTIDHAYFQEHQSTINHNFFTSSSSSNTATTAANKLEEHSDKPEDSPLPIASSISGSTPASSITSTSCTSSSSSSASMSSSCSSSNSGSTTTAPTTSSSAGAPTAPLTLAAAAETTLANVQAMLSTVAKLQQQQQELPVELNSNEMYQHVQHDWNFGDIKLSSSQSSGDQQRNLSHEAIDLMDVVQDADVIDDIMHNDVCHDVLGDEDEGDQEEDEDDEVVECMTEEQQLIDEDSEAVREIVDKLQQHQQQQNQQQHHQQLHIQDVVQLAQHSFMPQAHSEFGNDIGQEMLCDAVPMSAAEMEVSSTVITNSSNSNDSSNNISLCSSTNSLTINQMPHQASQQPQQNAQSNAQQQRQILVDSNGQIIGNFLLQQQRQQQQQQLLQQFTLQAAAAQQQQQQQQQHQQQQQQQQQATSSNSLGKTLPVALRNGTQQFLSPNLIAQQHQQQQQQQLEQHQQQATAQQKHQQIQQFALQQAQLHQRQLLAQAANNNLLQQQQQQQQNVALPTTQAKFIAKPLNIISMTRPANASPTTAATTANTASIPSAYANVVAVTGAQQQQSPPVPAPQQQTVQQQQLANHNSNMQQLPNVLTMKTLPPSGVPTTIAQQRLQPKMPTGKGRKATSNRLPPGAVNLERSYQICQAVIQNSPNRENLKAQLRPPAAILNQHQPTTTTAPAPINPVTLNVSTVAATPMSNITTATGSMAAAVAAAPPQNVLKQEELLVSGAVGAGALPAGLPPNVMGVGRPGVYKVIGPRMSGFPRKKYVQRKPSPTTLIRHVFSPGPGGATATAQQLQMLQQHHQSTTSPVPVQNPQQPAPEQLIHQNGNGQYVLVHRANVGAADNQAPRASSAPPMHQNQFVTVQNPLHSINGIPMGGRGRPASVDTTAGSGNVIAPPISATDALHHHHHEMQQQQQHQQPQPLGNVGAAANIVRRNIAAGPNIAYIDGSNTNSSAVALMEAGNNYIVTTNASPTAAPSPINQQPQSQPTGTQHQHPLLQLHQTGENTPPGNEATATANNCACSLNAMVICQQCGAFCHDDCIGAAKLCVACVIR

Target ORF information:

RefSeq Version	NM_166057.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster additional sex combs, transcript variant A (Asx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166057.2

ORF Insert Sequence:

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCCTATT TTCAAGAACA TCAATCCACT ATCAATCACA ACTTCTTCAC TTCATCTTCA 
TCGTCCAACA CGGCGACAAC TGCAGCTAAT AAACTGGAAG AGCATAGTGA TAAGCCGGAG 
GACTCACCGC TCCCCATTGC AAGCTCAATT TCTGGGTCGA CGCCGGCCAG TTCGATTACA 
TCGACCAGCT GCACATCGTC CTCATCTTCC TCCGCCTCAA TGTCATCTTC GTGCTCCAGT 
AGCAATTCCG GCTCGACGAC GACCGCGCCC ACAACTTCAT CGTCGGCCGG AGCACCAACG 
GCTCCACTGA CGCTGGCAGC AGCAGCTGAA ACTACGTTGG CAAATGTCCA AGCCATGCTT 
TCAACTGTGG CCAAGTTACA GCAGCAGCAA CAGGAGTTAC CCGTGGAGTT GAACTCCAAT 
GAAATGTACC AGCATGTGCA GCACGATTGG AACTTTGGCG ACATTAAGTT AAGCAGTTCG 
CAGTCAAGCG GAGATCAGCA GCGTAATCTA AGCCATGAAG CAATCGATCT GATGGATGTA 
GTGCAAGATG CCGACGTCAT CGACGACATT ATGCACAATG ATGTGTGCCA CGATGTTCTC 
GGTGACGAAG ATGAGGGTGA TCAAGAGGAG GATGAAGACG ATGAAGTGGT AGAATGTATG 
ACGGAGGAAC AGCAGTTAAT TGATGAAGAC AGCGAAGCTG TCCGTGAAAT AGTGGACAAA 
CTGCAGCAGC ATCAGCAGCA ACAAAATCAG CAGCAGCATC ATCAACAGTT GCATATCCAG 
GATGTCGTCC AATTGGCTCA GCATTCGTTT ATGCCGCAGG CCCATAGTGA ATTTGGAAAT 
GATATCGGCC AGGAAATGCT CTGTGATGCT GTACCAATGT CTGCTGCCGA AATGGAAGTG 
TCCAGCACGG TGATAACCAA TAGTAGCAAT AGCAACGACA GCAGCAACAA CATAAGCCTA 
TGCAGCAGCA CCAACAGTCT TACCATTAAT CAAATGCCGC ACCAAGCATC GCAACAGCCG 
CAACAGAACG CTCAGTCCAA TGCACAGCAG CAAAGACAGA TATTGGTGGA TTCCAATGGT 
CAGATAATTG GCAACTTTTT GCTTCAGCAG CAACGCCAAC AACAGCAACA GCAACTACTA 
CAACAGTTCA CGCTGCAGGC GGCGGCAGCA CAGCAGCAGC AGCAACAACA ACAACAGCAC 
CAGCAACAGC AGCAGCAGCA ACAGCAAGCA ACAAGTAGCA ATTCCTTGGG CAAGACATTA 
CCAGTGGCTC TCCGCAACGG AACCCAACAG TTCTTGTCTC CTAACCTGAT CGCACAGCAA 
CATCAGCAGC AGCAGCAGCA ACAACTGGAG CAACATCAAC AGCAGGCTAC GGCACAACAA 
AAGCATCAGC AGATTCAGCA GTTTGCCTTG CAGCAGGCGC AACTACACCA GCGACAACTT 
CTCGCCCAGG CCGCCAACAA CAATCTGCTA CAACAGCAGC AACAACAGCA GCAAAATGTT 
GCACTACCAA CAACACAGGC TAAATTCATA GCAAAGCCAC TAAATATCAT CTCAATGACG 
CGTCCTGCCA ATGCATCACC TACCACAGCA GCAACGACGG CAAATACCGC ATCTATTCCG 
TCCGCCTATG CCAATGTTGT TGCTGTGACG GGCGCGCAAC AGCAGCAATC TCCGCCAGTA 
CCTGCCCCTC AGCAGCAGAC AGTCCAACAA CAACAGTTGG CAAATCACAA CAGTAATATG 
CAGCAGTTAC CCAACGTGCT GACTATGAAA ACTCTGCCAC CATCGGGAGT GCCAACCACC 
ATTGCCCAGC AAAGATTGCA GCCAAAAATG CCAACGGGAA AAGGGCGCAA AGCAACCAGC 
AATAGGTTAC CACCGGGTGC AGTAAATCTG GAGCGTAGCT ATCAAATCTG CCAGGCTGTT 
ATCCAGAATA GTCCAAACCG CGAGAATCTC AAGGCTCAGT TGCGACCGCC TGCGGCGATT 
CTCAACCAAC ATCAGCCGAC GACAACCACT GCGCCAGCAC CTATAAACCC TGTGACCTTG 
AATGTATCAA CGGTGGCTGC CACACCCATG TCCAACATAA CGACGGCCAC GGGTAGCATG 
GCAGCTGCAG TCGCAGCAGC ACCCCCACAA AATGTATTAA AGCAAGAGGA GCTGTTGGTC 
AGTGGAGCAG TTGGTGCTGG AGCTCTGCCC GCTGGATTGC CGCCGAATGT CATGGGTGTC 
GGACGTCCGG GAGTATACAA GGTTATTGGA CCGCGTATGA GTGGTTTCCC AAGAAAGAAA 
TATGTCCAAC GGAAGCCGTC ACCCACAACG CTCATCCGGC ATGTGTTTAG TCCCGGACCT 
GGAGGAGCTA CAGCCACCGC TCAGCAATTG CAAATGCTTC AGCAGCATCA CCAGTCGACG 
ACTTCGCCAG TGCCTGTACA AAATCCTCAG CAGCCAGCAC CGGAGCAGTT GATACACCAA 
AATGGCAATG GACAATATGT TCTCGTTCAT CGGGCGAATG TGGGTGCAGC TGACAATCAA 
GCGCCTAGAG CCTCCAGTGC CCCGCCAATG CATCAAAACC AGTTCGTTAC TGTTCAGAAT 
CCGCTGCACA GCATAAACGG CATTCCTATG GGTGGACGCG GGCGTCCAGC ATCGGTGGAC 
ACAACGGCTG GCAGCGGCAA CGTTATAGCA CCACCAATTT CCGCCACAGA TGCGTTGCAT 
CATCATCACC ACGAAATGCA GCAGCAGCAG CAACATCAGC AGCCGCAGCC GCTGGGCAAT 
GTAGGCGCAG CCGCGAATAT TGTGCGGCGC AATATCGCTG CAGGACCCAA CATCGCCTAC 
ATTGACGGCA GCAATACCAA CTCATCGGCC GTCGCGCTTA TGGAAGCTGG CAACAACTAC 
ATAGTGACGA CCAACGCGTC ACCAACTGCA GCACCTTCGC CGATCAACCA GCAGCCCCAA 
TCGCAACCAA CTGGGACTCA GCATCAGCAT CCATTGCTGC AATTGCATCA AACCGGGGAG 
AACACTCCGC CGGGCAATGA AGCTACGGCC ACGGCGAACA ACTGCGCCTG CTCACTCAAT 
GCGATGGTGA TCTGCCAACA GTGCGGAGCC TTTTGCCACG ACGACTGCAT CGGTGCGGCC 
AAGCTGTGTG TCGCCTGTGT GATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asx ( NM_001014527.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014527.1 Drosophila melanogaster additional sex combs, transcript variant B (Asx), mRNA.

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CloneID	OFa09984
Clone ID Related Accession (Same CDS sequence)	NM_001014527.2
Accession Version	NM_001014527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	additional sex combs, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001014527.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCCTATT TTCAAGAACA TCAATCCACT ATCAATCACA ACTTCTTCAC TTCATCTTCA 
TCGTCCAACA CGGCGACAAC TGCAGCTAAT AAACTGGAAG AGCATAGTGA TAAGCCGGAG 
GACTCACCGC TCCCCATTGC AAGCTCAATT TCTGGGTCGA CGCCGGCCAG TTCGATTACA 
TCGACCAGCT GCACATCGTC CTCATCTTCC TCCGCCTCAA TGTCATCTTC GTGCTCCAGT 
AGCAATTCCG GCTCGACGAC GACCGCGCCC ACAACTTCAT CGTCGGCCGG AGCACCAACG 
GCTCCACTGA CGCTGGCAGC AGCAGCTGAA ACTACGTTGG CAAATGTCCA AGCCATGCTT 
TCAACTGTGG CCAAGTTACA GCAGCAGCAA CAGGAGTTAC CCGTGGAGTT GAACTCCAAT 
GAAATGTACC AGCATGTGCA GCACGATTGG AACTTTGGCG ACATTAAGTT AAGCAGTTCG 
CAGTCAAGCG GAGATCAGCA GCGTAATCTA AGCCATGAAG CAATCGATCT GATGGATGTA 
GTGCAAGATG CCGACGTCAT CGACGACATT ATGCACAATG ATGTGTGCCA CGATGTTCTC 
GGTGACGAAG ATGAGGGTGA TCAAGAGGAG GATGAAGACG ATGAAGTGGT AGAATGTATG 
ACGGAGGAAC AGCAGTTAAT TGATGAAGAC AGCGAAGCTG TCCGTGAAAT AGTGGACAAA 
CTGCAGCAGC ATCAGCAGCA ACAAAATCAG CAGCAGCATC ATCAACAGTT GCATATCCAG 
GATGTCGTCC AATTGGCTCA GCATTCGTTT ATGCCGCAGG CCCATAGTGA ATTTGGAAAT 
GATATCGGCC AGGAAATGCT CTGTGATGCT GTACCAATGT CTGCTGCCGA AATGGAAGTG 
TCCAGCACGG TGATAACCAA TAGTAGCAAT AGCAACGACA GCAGCAACAA CATAAGCCTA 
TGCAGCAGCA CCAACAGTCT TACCATTAAT CAAATGCCGC ACCAAGCATC GCAACAGCCG 
CAACAGAACG CTCAGTCCAA TGCACAGCAG CAAAGACAGA TATTGGTGGA TTCCAATGGT 
CAGATAATTG GCAACTTTTT GCTTCAGCAG CAACGCCAAC AACAGCAACA GCAACTACTA 
CAACAGTTCA CGCTGCAGGC GGCGGCAGCA CAGCAGCAGC AGCAACAACA ACAACAGCAC 
CAGCAACAGC AGCAGCAGCA ACAGCAAGCA ACAAGTAGCA ATTCCTTGGG CAAGACATTA 
CCAGTGGCTC TCCGCAACGG AACCCAACAG TTCTTGTCTC CTAACCTGAT CGCACAGCAA 
CATCAGCAGC AGCAGCAGCA ACAACTGGAG CAACATCAAC AGCAGGCTAC GGCACAACAA 
AAGCATCAGC AGATTCAGCA GTTTGCCTTG CAGCAGGCGC AACTACACCA GCGACAACTT 
CTCGCCCAGG CCGCCAACAA CAATCTGCTA CAACAGCAGC AACAACAGCA GCAAAATGTT 
GCACTACCAA CAACACAGGC TAAATTCATA GCAAAGCCAC TAAATATCAT CTCAATGACG 
CGTCCTGCCA ATGCATCACC TACCACAGCA GCAACGACGG CAAATACCGC ATCTATTCCG 
TCCGCCTATG CCAATGTTGT TGCTGTGACG GGCGCGCAAC AGCAGCAATC TCCGCCAGTA 
CCTGCCCCTC AGCAGCAGAC AGTCCAACAA CAACAGTTGG CAAATCACAA CAGTAATATG 
CAGCAGTTAC CCAACGTGCT GACTATGAAA ACTCTGCCAC CATCGGGAGT GCCAACCACC 
ATTGCCCAGC AAAGATTGCA GCCAAAAATG CCAACGGGAA AAGGGCGCAA AGCAACCAGC 
AATAGGTTAC CACCGGGTGC AGTAAATCTG GAGCGTAGCT ATCAAATCTG CCAGGCTGTT 
ATCCAGAATA GTCCAAACCG CGAGAATCTC AAGGCTCAGT TGCGACCGCC TGCGGCGATT 
CTCAACCAAC ATCAGCCGAC GACAACCACT GCGCCAGCAC CTATAAACCC TGTGACCTTG 
AATGTATCAA CGGTGGCTGC CACACCCATG TCCAACATAA CGACGGCCAC GGGTAGCATG 
GCAGCTGCAG TCGCAGCAGC ACCCCCACAA AATGTATTAA AGCAAGAGGA GCTGTTGGTC 
AGTGGAGCAG TTGGTGCTGG AGCTCTGCCC GCTGGATTGC CGCCGAATGT CATGGGTGTC 
GGACGTCCGG GAGTATACAA GGTTATTGGA CCGCGTATGA GTGGTTTCCC AAGAAAGAAA 
TATGTCCAAC GGAAGCCGTC ACCCACAACG CTCATCCGGC ATGTGTTTAG TCCCGGACCT 
GGAGGAGCTA CAGCCACCGC TCAGCAATTG CAAATGCTTC AGCAGCATCA CCAGTCGACG 
ACTTCGCCAG TGCCTGTACA AAATCCTCAG CAGCCAGCAC CGGAGCAGTT GATACACCAA 
AATGGCAATG GACAATATGT TCTCGTTCAT CGGGCGAATG TGGGTGCAGC TGACAATCAA 
GCGCCTAGAG CCTCCAGTGC CCCGCCAATG CATCAAAACC AGTTCGTTAC TGTTCAGAAT 
CCGCTGCACA GCATAAACGG CATTCCTATG GGTGGACGCG GGCGTCCAGC ATCGGTGGAC 
ACAACGGCTG GCAGCGGCAA CGTTATAGCA CCACCAATTT CCGCCACAGA TGCGTTGCAT 
CATCATCACC ACGAAATGCA GCAGCAGCAG CAACATCAGC AGCCGCAGCC GCTGGGCAAT 
GTAGGCGCAG CCGCGAATAT TGTGCGGCGC AATATCGCTG CAGGTAAATT GGTGTATTAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014527.1
CDS	163..4845
Translation	MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRRHLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAISPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLYQYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLKHFEPFWGEKNSRGKDKDKLESDCKNQKLSASIKSEPKPPATSQQKPLQQATCDNETELKFDLSTKCETTSAKTTVAVAVADKSSTFPPTGSQNNVLNEQQRRVLKRPSSSPSQRKQAPTTIATINLDDDLDELPSTSKDSKQPKMDEIVPNASGNVVAAPMVDVVDHSAVEMKIKDEQQHQRQHQPLINSTCDKIEPSECSKEMIVAMKQVDSKEDVDSIASAAAMPAIAAVTPHTPKPEALAPNPDVANQFVSYLQNVELAAETKAPLDNSNEADITTGTNSHDFVFSDTIDHAYFQEHQSTINHNFFTSSSSSNTATTAANKLEEHSDKPEDSPLPIASSISGSTPASSITSTSCTSSSSSSASMSSSCSSSNSGSTTTAPTTSSSAGAPTAPLTLAAAAETTLANVQAMLSTVAKLQQQQQELPVELNSNEMYQHVQHDWNFGDIKLSSSQSSGDQQRNLSHEAIDLMDVVQDADVIDDIMHNDVCHDVLGDEDEGDQEEDEDDEVVECMTEEQQLIDEDSEAVREIVDKLQQHQQQQNQQQHHQQLHIQDVVQLAQHSFMPQAHSEFGNDIGQEMLCDAVPMSAAEMEVSSTVITNSSNSNDSSNNISLCSSTNSLTINQMPHQASQQPQQNAQSNAQQQRQILVDSNGQIIGNFLLQQQRQQQQQQLLQQFTLQAAAAQQQQQQQQQHQQQQQQQQQATSSNSLGKTLPVALRNGTQQFLSPNLIAQQHQQQQQQQLEQHQQQATAQQKHQQIQQFALQQAQLHQRQLLAQAANNNLLQQQQQQQQNVALPTTQAKFIAKPLNIISMTRPANASPTTAATTANTASIPSAYANVVAVTGAQQQQSPPVPAPQQQTVQQQQLANHNSNMQQLPNVLTMKTLPPSGVPTTIAQQRLQPKMPTGKGRKATSNRLPPGAVNLERSYQICQAVIQNSPNRENLKAQLRPPAAILNQHQPTTTTAPAPINPVTLNVSTVAATPMSNITTATGSMAAAVAAAPPQNVLKQEELLVSGAVGAGALPAGLPPNVMGVGRPGVYKVIGPRMSGFPRKKYVQRKPSPTTLIRHVFSPGPGGATATAQQLQMLQQHHQSTTSPVPVQNPQQPAPEQLIHQNGNGQYVLVHRANVGAADNQAPRASSAPPMHQNQFVTVQNPLHSINGIPMGGRGRPASVDTTAGSGNVIAPPISATDALHHHHHEMQQQQQHQQPQPLGNVGAAANIVRRNIAAGKLVYY

Target ORF information:

RefSeq Version	NM_001014527.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster additional sex combs, transcript variant B (Asx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014527.2

ORF Insert Sequence:

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCCTATT TTCAAGAACA TCAATCCACT ATCAATCACA ACTTCTTCAC TTCATCTTCA 
TCGTCCAACA CGGCGACAAC TGCAGCTAAT AAACTGGAAG AGCATAGTGA TAAGCCGGAG 
GACTCACCGC TCCCCATTGC AAGCTCAATT TCTGGGTCGA CGCCGGCCAG TTCGATTACA 
TCGACCAGCT GCACATCGTC CTCATCTTCC TCCGCCTCAA TGTCATCTTC GTGCTCCAGT 
AGCAATTCCG GCTCGACGAC GACCGCGCCC ACAACTTCAT CGTCGGCCGG AGCACCAACG 
GCTCCACTGA CGCTGGCAGC AGCAGCTGAA ACTACGTTGG CAAATGTCCA AGCCATGCTT 
TCAACTGTGG CCAAGTTACA GCAGCAGCAA CAGGAGTTAC CCGTGGAGTT GAACTCCAAT 
GAAATGTACC AGCATGTGCA GCACGATTGG AACTTTGGCG ACATTAAGTT AAGCAGTTCG 
CAGTCAAGCG GAGATCAGCA GCGTAATCTA AGCCATGAAG CAATCGATCT GATGGATGTA 
GTGCAAGATG CCGACGTCAT CGACGACATT ATGCACAATG ATGTGTGCCA CGATGTTCTC 
GGTGACGAAG ATGAGGGTGA TCAAGAGGAG GATGAAGACG ATGAAGTGGT AGAATGTATG 
ACGGAGGAAC AGCAGTTAAT TGATGAAGAC AGCGAAGCTG TCCGTGAAAT AGTGGACAAA 
CTGCAGCAGC ATCAGCAGCA ACAAAATCAG CAGCAGCATC ATCAACAGTT GCATATCCAG 
GATGTCGTCC AATTGGCTCA GCATTCGTTT ATGCCGCAGG CCCATAGTGA ATTTGGAAAT 
GATATCGGCC AGGAAATGCT CTGTGATGCT GTACCAATGT CTGCTGCCGA AATGGAAGTG 
TCCAGCACGG TGATAACCAA TAGTAGCAAT AGCAACGACA GCAGCAACAA CATAAGCCTA 
TGCAGCAGCA CCAACAGTCT TACCATTAAT CAAATGCCGC ACCAAGCATC GCAACAGCCG 
CAACAGAACG CTCAGTCCAA TGCACAGCAG CAAAGACAGA TATTGGTGGA TTCCAATGGT 
CAGATAATTG GCAACTTTTT GCTTCAGCAG CAACGCCAAC AACAGCAACA GCAACTACTA 
CAACAGTTCA CGCTGCAGGC GGCGGCAGCA CAGCAGCAGC AGCAACAACA ACAACAGCAC 
CAGCAACAGC AGCAGCAGCA ACAGCAAGCA ACAAGTAGCA ATTCCTTGGG CAAGACATTA 
CCAGTGGCTC TCCGCAACGG AACCCAACAG TTCTTGTCTC CTAACCTGAT CGCACAGCAA 
CATCAGCAGC AGCAGCAGCA ACAACTGGAG CAACATCAAC AGCAGGCTAC GGCACAACAA 
AAGCATCAGC AGATTCAGCA GTTTGCCTTG CAGCAGGCGC AACTACACCA GCGACAACTT 
CTCGCCCAGG CCGCCAACAA CAATCTGCTA CAACAGCAGC AACAACAGCA GCAAAATGTT 
GCACTACCAA CAACACAGGC TAAATTCATA GCAAAGCCAC TAAATATCAT CTCAATGACG 
CGTCCTGCCA ATGCATCACC TACCACAGCA GCAACGACGG CAAATACCGC ATCTATTCCG 
TCCGCCTATG CCAATGTTGT TGCTGTGACG GGCGCGCAAC AGCAGCAATC TCCGCCAGTA 
CCTGCCCCTC AGCAGCAGAC AGTCCAACAA CAACAGTTGG CAAATCACAA CAGTAATATG 
CAGCAGTTAC CCAACGTGCT GACTATGAAA ACTCTGCCAC CATCGGGAGT GCCAACCACC 
ATTGCCCAGC AAAGATTGCA GCCAAAAATG CCAACGGGAA AAGGGCGCAA AGCAACCAGC 
AATAGGTTAC CACCGGGTGC AGTAAATCTG GAGCGTAGCT ATCAAATCTG CCAGGCTGTT 
ATCCAGAATA GTCCAAACCG CGAGAATCTC AAGGCTCAGT TGCGACCGCC TGCGGCGATT 
CTCAACCAAC ATCAGCCGAC GACAACCACT GCGCCAGCAC CTATAAACCC TGTGACCTTG 
AATGTATCAA CGGTGGCTGC CACACCCATG TCCAACATAA CGACGGCCAC GGGTAGCATG 
GCAGCTGCAG TCGCAGCAGC ACCCCCACAA AATGTATTAA AGCAAGAGGA GCTGTTGGTC 
AGTGGAGCAG TTGGTGCTGG AGCTCTGCCC GCTGGATTGC CGCCGAATGT CATGGGTGTC 
GGACGTCCGG GAGTATACAA GGTTATTGGA CCGCGTATGA GTGGTTTCCC AAGAAAGAAA 
TATGTCCAAC GGAAGCCGTC ACCCACAACG CTCATCCGGC ATGTGTTTAG TCCCGGACCT 
GGAGGAGCTA CAGCCACCGC TCAGCAATTG CAAATGCTTC AGCAGCATCA CCAGTCGACG 
ACTTCGCCAG TGCCTGTACA AAATCCTCAG CAGCCAGCAC CGGAGCAGTT GATACACCAA 
AATGGCAATG GACAATATGT TCTCGTTCAT CGGGCGAATG TGGGTGCAGC TGACAATCAA 
GCGCCTAGAG CCTCCAGTGC CCCGCCAATG CATCAAAACC AGTTCGTTAC TGTTCAGAAT 
CCGCTGCACA GCATAAACGG CATTCCTATG GGTGGACGCG GGCGTCCAGC ATCGGTGGAC 
ACAACGGCTG GCAGCGGCAA CGTTATAGCA CCACCAATTT CCGCCACAGA TGCGTTGCAT 
CATCATCACC ACGAAATGCA GCAGCAGCAG CAACATCAGC AGCCGCAGCC GCTGGGCAAT 
GTAGGCGCAG CCGCGAATAT TGTGCGGCGC AATATCGCTG CAGGTAAATT GGTGTATTAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asx ( NM_001274041.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260970.1 Drosophila melanogaster additional sex combs, transcript variant D (Asx), mRNA.

Price & Availability↑

CloneID	OFa12107
Clone ID Related Accession (Same CDS sequence)	NM_001274041.1
Accession Version	NM_001274041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	additional sex combs, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCGGCGA CAACTGCAGC TAATAAACTG GAAGAGCATA GTGATAAGCC GGAGGACTCA 
CCGCTCCCCA TTGCAAGCTC AATTTCTGGG TCGACGCCGG CCAGTTCGAT TACATCGACC 
AGCTGCACAT CGTCCTCATC TTCCTCCGCC TCAATGTCAT CTTCGTGCTC CAGTAGCAAT 
TCCGGCTCGA CGACGACCGC GCCCACAACT TCATCGTCGG CCGGAGCACC AACGGCTCCA 
CTGACGCTGG CAGCAGCAGC TGAAACTACG TTGGCAAATG TCCAAGCCAT GCTTTCAACT 
GTGGCCAAGT TACAGCAGCA GCAACAGGAG TTACCCGTGG AGTTGAACTC CAATGAAATG 
TACCAGCATG TGCAGCACGA TTGGAACTTT GGCGACATTA AGTTAAGCAG TTCGCAGTCA 
AGCGGAGATC AGCAGCGTAA TCTAAGCCAT GAAGCAATCG ATCTGATGGA TGTAGTGCAA 
GATGCCGACG TCATCGACGA CATTATGCAC AATGATGTGT GCCACGATGT TCTCGGTGAC 
GAAGATGAGG GTGATCAAGA GGAGGATGAA GACGATGAAG TGGTAGAATG TATGACGGAG 
GAACAGCAGT TAATTGATGA AGACAGCGAA GCTGTCCGTG AAATAGTGGA CAAACTGCAG 
CAGCATCAGC AGCAACAAAA TCAGCAGCAG CATCATCAAC AGTTGCATAT CCAGGATGTC 
GTCCAATTGG CTCAGCATTC GTTTATGCCG CAGGCCCATA GTGAATTTGG AAATGATATC 
GGCCAGGAAA TGCTCTGTGA TGCTGTACCA ATGTCTGCTG CCGAAATGGA AGTGTCCAGC 
ACGGTGATAA CCAATAGTAG CAATAGCAAC GACAGCAGCA ACAACATAAG CCTATGCAGC 
AGCACCAACA GTCTTACCAT TAATCAAATG CCGCACCAAG CATCGCAACA GCCGCAACAG 
AACGCTCAGT CCAATGCACA GCAGCAAAGA CAGATATTGG TGGATTCCAA TGGTCAGATA 
ATTGGCAACT TTTTGCTTCA GCAGCAACGC CAACAACAGC AACAGCAACT ACTACAACAG 
TTCACGCTGC AGGCGGCGGC AGCACAGCAG CAGCAGCAAC AACAACAACA GCACCAGCAA 
CAGCAGCAGC AGCAACAGCA AGCAACAAGT AGCAATTCCT TGGGCAAGAC ATTACCAGTG 
GCTCTCCGCA ACGGAACCCA ACAGTTCTTG TCTCCTAACC TGATCGCACA GCAACATCAG 
CAGCAGCAGC AGCAACAACT GGAGCAACAT CAACAGCAGG CTACGGCACA ACAAAAGCAT 
CAGCAGATTC AGCAGTTTGC CTTGCAGCAG GCGCAACTAC ACCAGCGACA ACTTCTCGCC 
CAGGCCGCCA ACAACAATCT GCTACAACAG CAGCAACAAC AGCAGCAAAA TGTTGCACTA 
CCAACAACAC AGGCTAAATT CATAGCAAAG CCACTAAATA TCATCTCAAT GACGCGTCCT 
GCCAATGCAT CACCTACCAC AGCAGCAACG ACGGCAAATA CCGCATCTAT TCCGTCCGCC 
TATGCCAATG TTGTTGCTGT GACGGGCGCG CAACAGCAGC AATCTCCGCC AGTACCTGCC 
CCTCAGCAGC AGACAGTCCA ACAACAACAG TTGGCAAATC ACAACAGTAA TATGCAGCAG 
TTACCCAACG TGCTGACTAT GAAAACTCTG CCACCATCGG GAGTGCCAAC CACCATTGCC 
CAGCAAAGAT TGCAGCCAAA AATGCCAACG GGAAAAGGGC GCAAAGCAAC CAGCAATAGG 
TTACCACCGG GTGCAGTAAA TCTGGAGCGT AGCTATCAAA TCTGCCAGGC TGTTATCCAG 
AATAGTCCAA ACCGCGAGAA TCTCAAGGCT CAGTTGCGAC CGCCTGCGGC GATTCTCAAC 
CAACATCAGC CGACGACAAC CACTGCGCCA GCACCTATAA ACCCTGTGAC CTTGAATGTA 
TCAACGGTGG CTGCCACACC CATGTCCAAC ATAACGACGG CCACGGGTAG CATGGCAGCT 
GCAGTCGCAG CAGCACCCCC ACAAAATGTA TTAAAGCAAG AGGAGCTGTT GGTCAGTGGA 
GCAGTTGGTG CTGGAGCTCT GCCCGCTGGA TTGCCGCCGA ATGTCATGGG TGTCGGACGT 
CCGGGAGTAT ACAAGGTTAT TGGACCGCGT ATGAGTGGTT TCCCAAGAAA GAAATATGTC 
CAACGGAAGC CGTCACCCAC AACGCTCATC CGGCATGTGT TTAGTCCCGG ACCTGGAGGA 
GCTACAGCCA CCGCTCAGCA ATTGCAAATG CTTCAGCAGC ATCACCAGTC GACGACTTCG 
CCAGTGCCTG TACAAAATCC TCAGCAGCCA GCACCGGAGC AGTTGATACA CCAAAATGGC 
AATGGACAAT ATGTTCTCGT TCATCGGGCG AATGTGGGTG CAGCTGACAA TCAAGCGCCT 
AGAGCCTCCA GTGCCCCGCC AATGCATCAA AACCAGTTCG TTACTGTTCA GAATCCGCTG 
CACAGCATAA ACGGCATTCC TATGGGTGGA CGCGGGCGTC CAGCATCGGT GGACACAACG 
GCTGGCAGCG GCAACGTTAT AGCACCACCA ATTTCCGCCA CAGATGCGTT GCATCATCAT 
CACCACGAAA TGCAGCAGCA GCAGCAACAT CAGCAGCCGC AGCCGCTGGG CAATGTAGGC 
GCAGCCGCGA ATATTGTGCG GCGCAATATC GCTGCAGGTA AATTGGTGTA TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260970.1
CDS	693..5309
Translation	MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRRHLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAISPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLYQYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLKHFEPFWGEKNSRGKDKDKLESDCKNQKLSASIKSEPKPPATSQQKPLQQATCDNETELKFDLSTKCETTSAKTTVAVAVADKSSTFPPTGSQNNVLNEQQRRVLKRPSSSPSQRKQAPTTIATINLDDDLDELPSTSKDSKQPKMDEIVPNASGNVVAAPMVDVVDHSAVEMKIKDEQQHQRQHQPLINSTCDKIEPSECSKEMIVAMKQVDSKEDVDSIASAAAMPAIAAVTPHTPKPEALAPNPDVANQFVSYLQNVELAAETKAPLDNSNEADITTGTNSHDFVFSDTIDHAATTAANKLEEHSDKPEDSPLPIASSISGSTPASSITSTSCTSSSSSSASMSSSCSSSNSGSTTTAPTTSSSAGAPTAPLTLAAAAETTLANVQAMLSTVAKLQQQQQELPVELNSNEMYQHVQHDWNFGDIKLSSSQSSGDQQRNLSHEAIDLMDVVQDADVIDDIMHNDVCHDVLGDEDEGDQEEDEDDEVVECMTEEQQLIDEDSEAVREIVDKLQQHQQQQNQQQHHQQLHIQDVVQLAQHSFMPQAHSEFGNDIGQEMLCDAVPMSAAEMEVSSTVITNSSNSNDSSNNISLCSSTNSLTINQMPHQASQQPQQNAQSNAQQQRQILVDSNGQIIGNFLLQQQRQQQQQQLLQQFTLQAAAAQQQQQQQQQHQQQQQQQQQATSSNSLGKTLPVALRNGTQQFLSPNLIAQQHQQQQQQQLEQHQQQATAQQKHQQIQQFALQQAQLHQRQLLAQAANNNLLQQQQQQQQNVALPTTQAKFIAKPLNIISMTRPANASPTTAATTANTASIPSAYANVVAVTGAQQQQSPPVPAPQQQTVQQQQLANHNSNMQQLPNVLTMKTLPPSGVPTTIAQQRLQPKMPTGKGRKATSNRLPPGAVNLERSYQICQAVIQNSPNRENLKAQLRPPAAILNQHQPTTTTAPAPINPVTLNVSTVAATPMSNITTATGSMAAAVAAAPPQNVLKQEELLVSGAVGAGALPAGLPPNVMGVGRPGVYKVIGPRMSGFPRKKYVQRKPSPTTLIRHVFSPGPGGATATAQQLQMLQQHHQSTTSPVPVQNPQQPAPEQLIHQNGNGQYVLVHRANVGAADNQAPRASSAPPMHQNQFVTVQNPLHSINGIPMGGRGRPASVDTTAGSGNVIAPPISATDALHHHHHEMQQQQQHQQPQPLGNVGAAANIVRRNIAAGKLVYY

Target ORF information:

RefSeq Version	NM_001274041.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster additional sex combs, transcript variant D (Asx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274041.1

ORF Insert Sequence:

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCGGCGA CAACTGCAGC TAATAAACTG GAAGAGCATA GTGATAAGCC GGAGGACTCA 
CCGCTCCCCA TTGCAAGCTC AATTTCTGGG TCGACGCCGG CCAGTTCGAT TACATCGACC 
AGCTGCACAT CGTCCTCATC TTCCTCCGCC TCAATGTCAT CTTCGTGCTC CAGTAGCAAT 
TCCGGCTCGA CGACGACCGC GCCCACAACT TCATCGTCGG CCGGAGCACC AACGGCTCCA 
CTGACGCTGG CAGCAGCAGC TGAAACTACG TTGGCAAATG TCCAAGCCAT GCTTTCAACT 
GTGGCCAAGT TACAGCAGCA GCAACAGGAG TTACCCGTGG AGTTGAACTC CAATGAAATG 
TACCAGCATG TGCAGCACGA TTGGAACTTT GGCGACATTA AGTTAAGCAG TTCGCAGTCA 
AGCGGAGATC AGCAGCGTAA TCTAAGCCAT GAAGCAATCG ATCTGATGGA TGTAGTGCAA 
GATGCCGACG TCATCGACGA CATTATGCAC AATGATGTGT GCCACGATGT TCTCGGTGAC 
GAAGATGAGG GTGATCAAGA GGAGGATGAA GACGATGAAG TGGTAGAATG TATGACGGAG 
GAACAGCAGT TAATTGATGA AGACAGCGAA GCTGTCCGTG AAATAGTGGA CAAACTGCAG 
CAGCATCAGC AGCAACAAAA TCAGCAGCAG CATCATCAAC AGTTGCATAT CCAGGATGTC 
GTCCAATTGG CTCAGCATTC GTTTATGCCG CAGGCCCATA GTGAATTTGG AAATGATATC 
GGCCAGGAAA TGCTCTGTGA TGCTGTACCA ATGTCTGCTG CCGAAATGGA AGTGTCCAGC 
ACGGTGATAA CCAATAGTAG CAATAGCAAC GACAGCAGCA ACAACATAAG CCTATGCAGC 
AGCACCAACA GTCTTACCAT TAATCAAATG CCGCACCAAG CATCGCAACA GCCGCAACAG 
AACGCTCAGT CCAATGCACA GCAGCAAAGA CAGATATTGG TGGATTCCAA TGGTCAGATA 
ATTGGCAACT TTTTGCTTCA GCAGCAACGC CAACAACAGC AACAGCAACT ACTACAACAG 
TTCACGCTGC AGGCGGCGGC AGCACAGCAG CAGCAGCAAC AACAACAACA GCACCAGCAA 
CAGCAGCAGC AGCAACAGCA AGCAACAAGT AGCAATTCCT TGGGCAAGAC ATTACCAGTG 
GCTCTCCGCA ACGGAACCCA ACAGTTCTTG TCTCCTAACC TGATCGCACA GCAACATCAG 
CAGCAGCAGC AGCAACAACT GGAGCAACAT CAACAGCAGG CTACGGCACA ACAAAAGCAT 
CAGCAGATTC AGCAGTTTGC CTTGCAGCAG GCGCAACTAC ACCAGCGACA ACTTCTCGCC 
CAGGCCGCCA ACAACAATCT GCTACAACAG CAGCAACAAC AGCAGCAAAA TGTTGCACTA 
CCAACAACAC AGGCTAAATT CATAGCAAAG CCACTAAATA TCATCTCAAT GACGCGTCCT 
GCCAATGCAT CACCTACCAC AGCAGCAACG ACGGCAAATA CCGCATCTAT TCCGTCCGCC 
TATGCCAATG TTGTTGCTGT GACGGGCGCG CAACAGCAGC AATCTCCGCC AGTACCTGCC 
CCTCAGCAGC AGACAGTCCA ACAACAACAG TTGGCAAATC ACAACAGTAA TATGCAGCAG 
TTACCCAACG TGCTGACTAT GAAAACTCTG CCACCATCGG GAGTGCCAAC CACCATTGCC 
CAGCAAAGAT TGCAGCCAAA AATGCCAACG GGAAAAGGGC GCAAAGCAAC CAGCAATAGG 
TTACCACCGG GTGCAGTAAA TCTGGAGCGT AGCTATCAAA TCTGCCAGGC TGTTATCCAG 
AATAGTCCAA ACCGCGAGAA TCTCAAGGCT CAGTTGCGAC CGCCTGCGGC GATTCTCAAC 
CAACATCAGC CGACGACAAC CACTGCGCCA GCACCTATAA ACCCTGTGAC CTTGAATGTA 
TCAACGGTGG CTGCCACACC CATGTCCAAC ATAACGACGG CCACGGGTAG CATGGCAGCT 
GCAGTCGCAG CAGCACCCCC ACAAAATGTA TTAAAGCAAG AGGAGCTGTT GGTCAGTGGA 
GCAGTTGGTG CTGGAGCTCT GCCCGCTGGA TTGCCGCCGA ATGTCATGGG TGTCGGACGT 
CCGGGAGTAT ACAAGGTTAT TGGACCGCGT ATGAGTGGTT TCCCAAGAAA GAAATATGTC 
CAACGGAAGC CGTCACCCAC AACGCTCATC CGGCATGTGT TTAGTCCCGG ACCTGGAGGA 
GCTACAGCCA CCGCTCAGCA ATTGCAAATG CTTCAGCAGC ATCACCAGTC GACGACTTCG 
CCAGTGCCTG TACAAAATCC TCAGCAGCCA GCACCGGAGC AGTTGATACA CCAAAATGGC 
AATGGACAAT ATGTTCTCGT TCATCGGGCG AATGTGGGTG CAGCTGACAA TCAAGCGCCT 
AGAGCCTCCA GTGCCCCGCC AATGCATCAA AACCAGTTCG TTACTGTTCA GAATCCGCTG 
CACAGCATAA ACGGCATTCC TATGGGTGGA CGCGGGCGTC CAGCATCGGT GGACACAACG 
GCTGGCAGCG GCAACGTTAT AGCACCACCA ATTTCCGCCA CAGATGCGTT GCATCATCAT 
CACCACGAAA TGCAGCAGCA GCAGCAACAT CAGCAGCCGC AGCCGCTGGG CAATGTAGGC 
GCAGCCGCGA ATATTGTGCG GCGCAATATC GCTGCAGGTA AATTGGTGTA TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asx ( NM_001274040.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260969.1 Drosophila melanogaster additional sex combs, transcript variant C (Asx), mRNA.

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CloneID	OFa12108
Clone ID Related Accession (Same CDS sequence)	NM_001274040.1
Accession Version	NM_001274040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	additional sex combs, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCAGCCT ATTTTCAAGA ACATCAATCC ACTATCAATC ACAACTTCTT CACTTCATCT 
TCATCGTCCA ACACGGCGAC AACTGCAGCT AATAAACTGG AAGAGCATAG TGATAAGCCG 
GAGGACTCAC CGCTCCCCAT TGCAAGCTCA ATTTCTGGGT CGACGCCGGC CAGTTCGATT 
ACATCGACCA GCTGCACATC GTCCTCATCT TCCTCCGCCT CAATGTCATC TTCGTGCTCC 
AGTAGCAATT CCGGCTCGAC GACGACCGCG CCCACAACTT CATCGTCGGC CGGAGCACCA 
ACGGCTCCAC TGACGCTGGC AGCAGCAGCT GAAACTACGT TGGCAAATGT CCAAGCCATG 
CTTTCAACTG TGGCCAAGTT ACAGCAGCAG CAACAGGAGT TACCCGTGGA GTTGAACTCC 
AATGAAATGT ACCAGCATGT GCAGCACGAT TGGAACTTTG GCGACATTAA GTTAAGCAGT 
TCGCAGTCAA GCGGAGATCA GCAGCGTAAT CTAAGCCATG AAGCAATCGA TCTGATGGAT 
GTAGTGCAAG ATGCCGACGT CATCGACGAC ATTATGCACA ATGATGTGTG CCACGATGTT 
CTCGGTGACG AAGATGAGGG TGATCAAGAG GAGGATGAAG ACGATGAAGT GGTAGAATGT 
ATGACGGAGG AACAGCAGTT AATTGATGAA GACAGCGAAG CTGTCCGTGA AATAGTGGAC 
AAACTGCAGC AGCATCAGCA GCAACAAAAT CAGCAGCAGC ATCATCAACA GTTGCATATC 
CAGGATGTCG TCCAATTGGC TCAGCATTCG TTTATGCCGC AGGCCCATAG TGAATTTGGA 
AATGATATCG GCCAGGAAAT GCTCTGTGAT GCTGTACCAA TGTCTGCTGC CGAAATGGAA 
GTGTCCAGCA CGGTGATAAC CAATAGTAGC AATAGCAACG ACAGCAGCAA CAACATAAGC 
CTATGCAGCA GCACCAACAG TCTTACCATT AATCAAATGC CGCACCAAGC ATCGCAACAG 
CCGCAACAGA ACGCTCAGTC CAATGCACAG CAGCAAAGAC AGATATTGGT GGATTCCAAT 
GGTCAGATAA TTGGCAACTT TTTGCTTCAG CAGCAACGCC AACAACAGCA ACAGCAACTA 
CTACAACAGT TCACGCTGCA GGCGGCGGCA GCACAGCAGC AGCAGCAACA ACAACAACAG 
CACCAGCAAC AGCAGCAGCA GCAACAGCAA GCAACAAGTA GCAATTCCTT GGGCAAGACA 
TTACCAGTGG CTCTCCGCAA CGGAACCCAA CAGTTCTTGT CTCCTAACCT GATCGCACAG 
CAACATCAGC AGCAGCAGCA GCAACAACTG GAGCAACATC AACAGCAGGC TACGGCACAA 
CAAAAGCATC AGCAGATTCA GCAGTTTGCC TTGCAGCAGG CGCAACTACA CCAGCGACAA 
CTTCTCGCCC AGGCCGCCAA CAACAATCTG CTACAACAGC AGCAACAACA GCAGCAAAAT 
GTTGCACTAC CAACAACACA GGCTAAATTC ATAGCAAAGC CACTAAATAT CATCTCAATG 
ACGCGTCCTG CCAATGCATC ACCTACCACA GCAGCAACGA CGGCAAATAC CGCATCTATT 
CCGTCCGCCT ATGCCAATGT TGTTGCTGTG ACGGGCGCGC AACAGCAGCA ATCTCCGCCA 
GTACCTGCCC CTCAGCAGCA GACAGTCCAA CAACAACAGT TGGCAAATCA CAACAGTAAT 
ATGCAGCAGT TACCCAACGT GCTGACTATG AAAACTCTGC CACCATCGGG AGTGCCAACC 
ACCATTGCCC AGCAAAGATT GCAGCCAAAA ATGCCAACGG GAAAAGGGCG CAAAGCAACC 
AGCAATAGGT TACCACCGGG TGCAGTAAAT CTGGAGCGTA GCTATCAAAT CTGCCAGGCT 
GTTATCCAGA ATAGTCCAAA CCGCGAGAAT CTCAAGGCTC AGTTGCGACC GCCTGCGGCG 
ATTCTCAACC AACATCAGCC GACGACAACC ACTGCGCCAG CACCTATAAA CCCTGTGACC 
TTGAATGTAT CAACGGTGGC TGCCACACCC ATGTCCAACA TAACGACGGC CACGGGTAGC 
ATGGCAGCTG CAGTCGCAGC AGCACCCCCA CAAAATGTAT TAAAGCAAGA GGAGCTGTTG 
GTCAGTGGAG CAGTTGGTGC TGGAGCTCTG CCCGCTGGAT TGCCGCCGAA TGTCATGGGT 
GTCGGACGTC CGGGAGTATA CAAGGTTATT GGACCGCGTA TGAGTGGTTT CCCAAGAAAG 
AAATATGTCC AACGGAAGCC GTCACCCACA ACGCTCATCC GGCATGTGTT TAGTCCCGGA 
CCTGGAGGAG CTACAGCCAC CGCTCAGCAA TTGCAAATGC TTCAGCAGCA TCACCAGTCG 
ACGACTTCGC CAGTGCCTGT ACAAAATCCT CAGCAGCCAG CACCGGAGCA GTTGATACAC 
CAAAATGGCA ATGGACAATA TGTTCTCGTT CATCGGGCGA ATGTGGGTGC AGCTGACAAT 
CAAGCGCCTA GAGCCTCCAG TGCCCCGCCA ATGCATCAAA ACCAGTTCGT TACTGTTCAG 
AATCCGCTGC ACAGCATAAA CGGCATTCCT ATGGGTGGAC GCGGGCGTCC AGCATCGGTG 
GACACAACGG CTGGCAGCGG CAACGTTATA GCACCACCAA TTTCCGCCAC AGATGCGTTG 
CATCATCATC ACCACGAAAT GCAGCAGCAG CAGCAACATC AGCAGCCGCA GCCGCTGGGC 
AATGTAGGCG CAGCCGCGAA TATTGTGCGG CGCAATATCG CTGCAGGACC CAACATCGCC 
TACATTGACG GCAGCAATAC CAACTCATCG GCCGTCGCGC TTATGGAAGC TGGCAACAAC 
TACATAGTGA CGACCAACGC GTCACCAACT GCAGCACCTT CGCCGATCAA CCAGCAGCCC 
CAATCGCAAC CAACTGGGAC TCAGCATCAG CATCCATTGC TGCAATTGCA TCAAACCGGG 
GAGAACACTC CGCCGGGCAA TGAAGCTACG GCCACGGCGA ACAACTGCGC CTGCTCACTC 
AATGCGATGG TGATCTGCCA ACAGTGCGGA GCCTTTTGCC ACGACGACTG CATCGGTGCG 
GCCAAGCTGT GTGTCGCCTG TGTGATTAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260969.1
CDS	223..5235
Translation	MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRRHLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAISPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLYQYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLKHFEPFWGEKNSRGKDKDKLESDCKNQKLSASIKSEPKPPATSQQKPLQQATCDNETELKFDLSTKCETTSAKTTVAVAVADKSSTFPPTGSQNNVLNEQQRRVLKRPSSSPSQRKQAPTTIATINLDDDLDELPSTSKDSKQPKMDEIVPNASGNVVAAPMVDVVDHSAVEMKIKDEQQHQRQHQPLINSTCDKIEPSECSKEMIVAMKQVDSKEDVDSIASAAAMPAIAAVTPHTPKPEALAPNPDVANQFVSYLQNVELAAETKAPLDNSNEADITTGTNSHDFVFSDTIDHAAYFQEHQSTINHNFFTSSSSSNTATTAANKLEEHSDKPEDSPLPIASSISGSTPASSITSTSCTSSSSSSASMSSSCSSSNSGSTTTAPTTSSSAGAPTAPLTLAAAAETTLANVQAMLSTVAKLQQQQQELPVELNSNEMYQHVQHDWNFGDIKLSSSQSSGDQQRNLSHEAIDLMDVVQDADVIDDIMHNDVCHDVLGDEDEGDQEEDEDDEVVECMTEEQQLIDEDSEAVREIVDKLQQHQQQQNQQQHHQQLHIQDVVQLAQHSFMPQAHSEFGNDIGQEMLCDAVPMSAAEMEVSSTVITNSSNSNDSSNNISLCSSTNSLTINQMPHQASQQPQQNAQSNAQQQRQILVDSNGQIIGNFLLQQQRQQQQQQLLQQFTLQAAAAQQQQQQQQQHQQQQQQQQQATSSNSLGKTLPVALRNGTQQFLSPNLIAQQHQQQQQQQLEQHQQQATAQQKHQQIQQFALQQAQLHQRQLLAQAANNNLLQQQQQQQQNVALPTTQAKFIAKPLNIISMTRPANASPTTAATTANTASIPSAYANVVAVTGAQQQQSPPVPAPQQQTVQQQQLANHNSNMQQLPNVLTMKTLPPSGVPTTIAQQRLQPKMPTGKGRKATSNRLPPGAVNLERSYQICQAVIQNSPNRENLKAQLRPPAAILNQHQPTTTTAPAPINPVTLNVSTVAATPMSNITTATGSMAAAVAAAPPQNVLKQEELLVSGAVGAGALPAGLPPNVMGVGRPGVYKVIGPRMSGFPRKKYVQRKPSPTTLIRHVFSPGPGGATATAQQLQMLQQHHQSTTSPVPVQNPQQPAPEQLIHQNGNGQYVLVHRANVGAADNQAPRASSAPPMHQNQFVTVQNPLHSINGIPMGGRGRPASVDTTAGSGNVIAPPISATDALHHHHHEMQQQQQHQQPQPLGNVGAAANIVRRNIAAGPNIAYIDGSNTNSSAVALMEAGNNYIVTTNASPTAAPSPINQQPQSQPTGTQHQHPLLQLHQTGENTPPGNEATATANNCACSLNAMVICQQCGAFCHDDCIGAAKLCVACVIR

Target ORF information:

RefSeq Version	NM_001274040.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster additional sex combs, transcript variant C (Asx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274040.1

ORF Insert Sequence:

ATGAAAACCA TTACGCCGGA TACGACGACG ACGACGTCGT CGCAGCACCA ACAACTTCTC 
ATTCCACAAG CGGATCAGCA TCACCAGCCG ATGCTGCAGC AGCAATCCCT GTTGGCCGCT 
CCACCGCCTA CGATGATAAT GGAGCATGTG AATCTGGTGG ACGACGACGA GAAGGATCCT 
CTTGCGCTGG AGCAACTGGA AGTGTCACCG TCTACCAAGC ACACACACAG TCTCAGACGC 
CACCTGCCAC GCATTATCGT GAAACCAATA CCACCTGAGA AGAAACCGAT GGCGCCCAGC 
GAAGAGGCTG CCGTCAGTAC TGCTCCTGCC CCGCCCACGC GTTTAATATG CAGCCGACGC 
ATTCAGCAAC AGCAACAGGT CAAAGCGGCA GCAGCAGCGG CGGCGGCAGC AGCGGCGGCA 
GCAGCAGCAG CAGCGGCCGC AGCACAAGCC CAAGCCACCT CCAGCTATCC ATCCGCCATC 
TCACCCGGCA GCAAGGCGGG CACCTCTCAA GCTTCGACCA TGCGGGAGGT CCTGGCCTCC 
ATACCCGGTT TTAGTGTTAA GCCCCGTCGG AGGAGCAATA AGAAGCTGAC GACAGCAGCG 
CAGATTGAGC AAACAAAAGA CGGAAAGATC GACCTGGAGA CCCCCGACTC CATACTGGCC 
TCCACCAACT TAAGAGCGCT GTTGAACAAG CAAACGTTCT CGCTTTTGCC ACCGCTCTAT 
CAATACAATC TCATACAACT GCTGCCCAGC GTGGATCGCG AGGCCAGCGA ACTAGAGCAA 
CCTAGCAGTA GCGCAAGTGG AGGCAGTCCA TCGGAGGCTA TAAGGCTAAG TGCCTCCTGT 
CTAAACAACG AGTTTTTTGC CCGAGCCTGC TTGGAGTGGC GGGAACGACT AAGCGAAGGC 
GAGTTTACAC CCGAAAACCA GCTGAAACTT AAAACGGAAG CAGAGCGCGA AAAGAACAAG 
TTGGATCCCT GGAAGCTGAA ACACTTTGAG CCCTTCTGGG GCGAGAAGAA TTCTAGGGGG 
AAGGATAAGG ATAAGTTAGA AAGCGATTGC AAGAATCAGA AGCTTTCGGC AAGCATTAAG 
AGTGAGCCCA AACCACCAGC GACATCGCAA CAAAAACCAC TGCAACAAGC AACATGTGAT 
AATGAGACTG AATTAAAATT TGATTTGAGC ACAAAGTGCG AAACGACATC AGCTAAAACT 
ACAGTAGCAG TGGCAGTAGC AGATAAATCA AGCACGTTTC CACCCACTGG CAGCCAGAAT 
AATGTGCTTA ACGAACAGCA ACGCCGTGTC CTCAAACGTC CATCGAGCAG TCCTTCGCAG 
CGCAAGCAGG CACCCACAAC GATAGCGACC ATTAATTTGG ACGATGATCT CGACGAGCTG 
CCCAGCACAT CAAAGGACAG CAAGCAGCCG AAGATGGACG AAATTGTTCC TAATGCGTCA 
GGGAATGTTG TCGCAGCTCC AATGGTTGAT GTTGTGGATC ACTCTGCAGT CGAAATGAAA 
ATCAAGGACG AGCAACAGCA TCAGCGACAA CATCAGCCAC TTATCAACAG TACCTGTGAT 
AAAATTGAGC CATCTGAATG CAGTAAGGAG ATGATCGTTG CCATGAAACA AGTGGATTCT 
AAGGAAGATG TGGACTCCAT TGCATCGGCG GCTGCCATGC CAGCAATTGC TGCCGTTACC 
CCACATACAC CAAAGCCGGA AGCGTTAGCA CCAAATCCGG ATGTGGCTAA CCAATTTGTA 
AGCTACTTAC AAAACGTCGA ACTAGCAGCT GAAACCAAAG CGCCTTTGGA CAATTCAAAC 
GAGGCAGATA TAACGACAGG AACAAATAGC CATGATTTCG TTTTCTCAGA TACTATCGAT 
CACGCAGCCT ATTTTCAAGA ACATCAATCC ACTATCAATC ACAACTTCTT CACTTCATCT 
TCATCGTCCA ACACGGCGAC AACTGCAGCT AATAAACTGG AAGAGCATAG TGATAAGCCG 
GAGGACTCAC CGCTCCCCAT TGCAAGCTCA ATTTCTGGGT CGACGCCGGC CAGTTCGATT 
ACATCGACCA GCTGCACATC GTCCTCATCT TCCTCCGCCT CAATGTCATC TTCGTGCTCC 
AGTAGCAATT CCGGCTCGAC GACGACCGCG CCCACAACTT CATCGTCGGC CGGAGCACCA 
ACGGCTCCAC TGACGCTGGC AGCAGCAGCT GAAACTACGT TGGCAAATGT CCAAGCCATG 
CTTTCAACTG TGGCCAAGTT ACAGCAGCAG CAACAGGAGT TACCCGTGGA GTTGAACTCC 
AATGAAATGT ACCAGCATGT GCAGCACGAT TGGAACTTTG GCGACATTAA GTTAAGCAGT 
TCGCAGTCAA GCGGAGATCA GCAGCGTAAT CTAAGCCATG AAGCAATCGA TCTGATGGAT 
GTAGTGCAAG ATGCCGACGT CATCGACGAC ATTATGCACA ATGATGTGTG CCACGATGTT 
CTCGGTGACG AAGATGAGGG TGATCAAGAG GAGGATGAAG ACGATGAAGT GGTAGAATGT 
ATGACGGAGG AACAGCAGTT AATTGATGAA GACAGCGAAG CTGTCCGTGA AATAGTGGAC 
AAACTGCAGC AGCATCAGCA GCAACAAAAT CAGCAGCAGC ATCATCAACA GTTGCATATC 
CAGGATGTCG TCCAATTGGC TCAGCATTCG TTTATGCCGC AGGCCCATAG TGAATTTGGA 
AATGATATCG GCCAGGAAAT GCTCTGTGAT GCTGTACCAA TGTCTGCTGC CGAAATGGAA 
GTGTCCAGCA CGGTGATAAC CAATAGTAGC AATAGCAACG ACAGCAGCAA CAACATAAGC 
CTATGCAGCA GCACCAACAG TCTTACCATT AATCAAATGC CGCACCAAGC ATCGCAACAG 
CCGCAACAGA ACGCTCAGTC CAATGCACAG CAGCAAAGAC AGATATTGGT GGATTCCAAT 
GGTCAGATAA TTGGCAACTT TTTGCTTCAG CAGCAACGCC AACAACAGCA ACAGCAACTA 
CTACAACAGT TCACGCTGCA GGCGGCGGCA GCACAGCAGC AGCAGCAACA ACAACAACAG 
CACCAGCAAC AGCAGCAGCA GCAACAGCAA GCAACAAGTA GCAATTCCTT GGGCAAGACA 
TTACCAGTGG CTCTCCGCAA CGGAACCCAA CAGTTCTTGT CTCCTAACCT GATCGCACAG 
CAACATCAGC AGCAGCAGCA GCAACAACTG GAGCAACATC AACAGCAGGC TACGGCACAA 
CAAAAGCATC AGCAGATTCA GCAGTTTGCC TTGCAGCAGG CGCAACTACA CCAGCGACAA 
CTTCTCGCCC AGGCCGCCAA CAACAATCTG CTACAACAGC AGCAACAACA GCAGCAAAAT 
GTTGCACTAC CAACAACACA GGCTAAATTC ATAGCAAAGC CACTAAATAT CATCTCAATG 
ACGCGTCCTG CCAATGCATC ACCTACCACA GCAGCAACGA CGGCAAATAC CGCATCTATT 
CCGTCCGCCT ATGCCAATGT TGTTGCTGTG ACGGGCGCGC AACAGCAGCA ATCTCCGCCA 
GTACCTGCCC CTCAGCAGCA GACAGTCCAA CAACAACAGT TGGCAAATCA CAACAGTAAT 
ATGCAGCAGT TACCCAACGT GCTGACTATG AAAACTCTGC CACCATCGGG AGTGCCAACC 
ACCATTGCCC AGCAAAGATT GCAGCCAAAA ATGCCAACGG GAAAAGGGCG CAAAGCAACC 
AGCAATAGGT TACCACCGGG TGCAGTAAAT CTGGAGCGTA GCTATCAAAT CTGCCAGGCT 
GTTATCCAGA ATAGTCCAAA CCGCGAGAAT CTCAAGGCTC AGTTGCGACC GCCTGCGGCG 
ATTCTCAACC AACATCAGCC GACGACAACC ACTGCGCCAG CACCTATAAA CCCTGTGACC 
TTGAATGTAT CAACGGTGGC TGCCACACCC ATGTCCAACA TAACGACGGC CACGGGTAGC 
ATGGCAGCTG CAGTCGCAGC AGCACCCCCA CAAAATGTAT TAAAGCAAGA GGAGCTGTTG 
GTCAGTGGAG CAGTTGGTGC TGGAGCTCTG CCCGCTGGAT TGCCGCCGAA TGTCATGGGT 
GTCGGACGTC CGGGAGTATA CAAGGTTATT GGACCGCGTA TGAGTGGTTT CCCAAGAAAG 
AAATATGTCC AACGGAAGCC GTCACCCACA ACGCTCATCC GGCATGTGTT TAGTCCCGGA 
CCTGGAGGAG CTACAGCCAC CGCTCAGCAA TTGCAAATGC TTCAGCAGCA TCACCAGTCG 
ACGACTTCGC CAGTGCCTGT ACAAAATCCT CAGCAGCCAG CACCGGAGCA GTTGATACAC 
CAAAATGGCA ATGGACAATA TGTTCTCGTT CATCGGGCGA ATGTGGGTGC AGCTGACAAT 
CAAGCGCCTA GAGCCTCCAG TGCCCCGCCA ATGCATCAAA ACCAGTTCGT TACTGTTCAG 
AATCCGCTGC ACAGCATAAA CGGCATTCCT ATGGGTGGAC GCGGGCGTCC AGCATCGGTG 
GACACAACGG CTGGCAGCGG CAACGTTATA GCACCACCAA TTTCCGCCAC AGATGCGTTG 
CATCATCATC ACCACGAAAT GCAGCAGCAG CAGCAACATC AGCAGCCGCA GCCGCTGGGC 
AATGTAGGCG CAGCCGCGAA TATTGTGCGG CGCAATATCG CTGCAGGACC CAACATCGCC 
TACATTGACG GCAGCAATAC CAACTCATCG GCCGTCGCGC TTATGGAAGC TGGCAACAAC 
TACATAGTGA CGACCAACGC GTCACCAACT GCAGCACCTT CGCCGATCAA CCAGCAGCCC 
CAATCGCAAC CAACTGGGAC TCAGCATCAG CATCCATTGC TGCAATTGCA TCAAACCGGG 
GAGAACACTC CGCCGGGCAA TGAAGCTACG GCCACGGCGA ACAACTGCGC CTGCTCACTC 
AATGCGATGG TGATCTGCCA ACAGTGCGGA GCCTTTTGCC ACGACGACTG CATCGGTGCG 
GCCAAGCTGT GTGTCGCCTG TGTGATTAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Asx cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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