Rcd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rcd1
Entrez Gene ID	36560
Full Name	Reduction in Cnn dots 1
Synonyms	CG8233,Dmel\CG8233,NSL3,Nsl3,cg8233
General protein information	Preferred Names Reduction in Cnn dots 1 Names CG8233 gene product from transcript CG8233-RA CG8233-PA CG8233-PB CG8233-PC CG8233-PD CG8233-PE CG8233-PF CG8233-PG CG8233-PH Rcd1-PA Rcd1-PB Rcd1-PC Rcd1-PD Rcd1-PE Rcd1-PF Rcd1-PG Rcd1-PH non-specific lethal 3 reduction in Cnn dots 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50E1-50E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274033.2	NP_001260962.1	reduction in Cnn dots 1, isoform D
NM_001274035.2	NP_001260964.1	reduction in Cnn dots 1, isoform F
NM_166032.3	NP_725358.1	reduction in Cnn dots 1, isoform C
NM_001299475.1	NP_001286404.1	reduction in Cnn dots 1, isoform H
NM_001274034.2	NP_001260963.1	reduction in Cnn dots 1, isoform E
NM_001274036.2	NP_001260965.1	reduction in Cnn dots 1, isoform G
NM_166031.3	NP_725357.2	reduction in Cnn dots 1, isoform A
NM_137083.4	NP_610927.3	reduction in Cnn dots 1, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Rcd1	NP_001260962.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004479	XP_313778.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rcd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rcd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12536	NM_166032.3 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant C (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09927	NM_001274035.2 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant F (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa09928	NM_001274033.2 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant D (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa09929	NM_001274036.2 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant G (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa09930	NM_001274034.2 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant E (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12535	NM_166031.3 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant A (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12538	NM_137083.4 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant B (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21799	NM_001299475.1 Latest version!	Drosophila melanogaster reduction in Cnn dots 1, transcript variant H (Rcd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rcd1 ( NM_166032.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725358.1 Drosophila melanogaster reduction in Cnn dots 1, transcript variant C (Rcd1), mRNA.

Price & Availability↑

CloneID	OFa12536
Clone ID Related Accession (Same CDS sequence)	NM_166032.3
Accession Version	NM_166032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166032.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCACA GCTATATGCG AGATACGCCA GTGCGCAGCG ATGTTCACAA CGGCCTAACG 
CCCGCTAGGA ACATTCTGGT CCGCCATCCG CCACAGTGTC CCAGTTGCCA CACATATCCG 
CCGCAGCACG AGGAGCTGTC AGAGATTCAG CAGGCCCATG TGCCGCCCTA TGATGAGGTA 
GTCGCCAAGG AGGCAATGAA TGAATGCGCT CGTATTGCAA AATACGTAAA GAACAACAAT 
TCGGATGAGC AAGATTGGGT GGCGCGCGTT AATAAATTTA ATTGGACGCC CATGCAGGAG 
ATGGTTTTCG AGAAGGTCTG CTCAATTCTG GACCAGGATC AGTTGGCACG CTTGGCCAAC 
GACAAACGTC AGCACGAGCC TATCCATCGT CGCATAAGCT CGGACAAATC CGCGTCCAGG 
TTTCGCAAAC TTCTAGCCAG CGTGGCTTGG GAGTCACGCA TTACGCAGTG GATCCATGCT 
CTGCTCATGG AGCACTTGTC GCCTTCGTAT ATGGCCTCTT ATCTGGAAAT TCTTCAGACT 
CTGAAGACCA AGTTGCCCAC TTTAGTGGAC AAGATGCTGT TTGGTAGGCC CTTAAATAAT 
AGTCAGGAAC TGCTGGCACC GGTGATGAAA AAACGATGGG AACCAAATAT CATGCCCAAG 
TCCCGTCAGC TAACGCATAA CGCCATCATG GTGGTGCTGC CCAACATGCC GACAACTGGA 
ACAGTTTCGG ACCGCATGCA GAAGTGGTAC CAGGCATTAT CTACTATCAC TCAGGTGGTT 
CAGATTTCGC TGCCGAATAC AAACAACAGA ATTGGGAATC AGAATCTGGA CCATGTGGCC 
GAAACCATTG TGTCTATCAC ACGCGTCAAA ATCAACGAAT TGCGCACAGA AAACCCCAGT 
CGTGGCATCA TACTCGTTGG ATTTAATGCT GGAGCTGCCT TGGCTCTACA GGTTGCTCTA 
TCGGAGAGCG TTGCCTGTGT GGTTTGCATG GGATTTGCTT ACAACACAAT ACGTGGGCCA 
CGCGGAACCC CCGATGACCG CATGTTGGAC ATAAAAGCGC CGATACTCTT TGTCATCGGA 
CAAAATTCCG CACGCACAAG TCAGGAGGAA ATGGAAGGGT TACGCGAACG AATGCAGTCC 
GAATCTTCGT TGGTCGTAGT GGGTAGTGCA GATGATGCAC TGCGTGTGCC TAAGAGCAAG 
CGGCGAATAG AAGGCGTTAC ACAGTCGATG GTTGATTACA TGGTTGTCGA GGAAATCTTT 
GAGTTTGTGA ACAGAACGCT AAGCAATCCA CCTGGACCGC GTATGCCCAC ATGTTTAATG 
CATCAGCAGG GCTACCAGCG ACTGCCAAAG CAGTCACCTC ACATCCTGGC CGATGGAAAC 
GCAAACAAGG CTGTTCAGCA GATGCGTAAG CGAAAGATGG ACGGTGGTTT AGACGACTCA 
ATGGGTCAGC CTTCCAAAAC AAAGTTTGTT CCTCACAATG ATGCCCAGGA CTATGGAGAT 
GCCCAAACCA CTGCAGGTGG TTCAGCGAGC CTGCCAAAGG TGATAACTCC CGGTGTTTTG 
GGAAAACAAC TGCCGCCCGT CAATCTCGCA GCTGGCACCA AGATTAAGAT GATCCCATCC 
AGCCAGTTCG TTCAAATCAA ATCGCTACCG TCACAGAGCA AACTCATTAA CTACACGCTA 
AACAAACCGA GTGTCGGGAC AAGCAGCACC GCCAACATTG GCAGCGTGGT GAAGACCCTA 
CCATGCTCAT CCGTTAGTGG CCAGCAGATC ATCACCTTAA AAACGCCCTC GGGACAAACG 
CAGCAGTTTG CAACAGCAGC CACATCAACA GGTGCCGCTG GTTCATCGAC ATCAACAGGA 
ACGGGACAGA AAAAGTACAC AGTTTTTAAG AACGCCAACG GCATGACCAT GCTGCATCTC 
ACCAAGAGCG TACCCACTAC AGGTTCTAGC AGCGGCTCTG CGAATATGGA TTTGTCCAAC 
ATTATCGATA TGCCCATTGT TTTTGCCGAT AACGAAGGCA ATATTCCCGA GCACCAACAG 
GCGGATAAGG ATATTAAACC AGCCCCCATT CGAAGTGCGA ATAAGAGCCC GTTGATCATC 
AGTCAGAAAA TCATTAAGGA GGCAAATAAG CCCTCGGGCA TGGTTACCAG TGGCAGCATT 
ACTGCCGTCA GCAAGCCGGG GAATATTGTG CTTAACAAAG GCCTTCAGCA ATTGCTAAAT 
TCATCGCCCG GTGGAACACT TGCCACTCAG AATAAAGTTG TGTACCTCAA CCGGAGTACA 
ATTAAGCCAA TGGGAAATGT GGCGCCTGGT ACTCATCGTG TCCCTATAAG GATTGTGAGC 
ACCCCAAACA CCGGAGGTGC AGTGACGACC ACAGCTTCCA GTAGTCCCAT TATGGTTGAT 
CCAGCCACAG GATCCAAGGT TGCCAAGACC GTCAATGTGC AGACAATCAA GCCTACTGGT 
TCCGTTCTGG CTCAAGGCAC CATCCGTCAA GCGGGCAACG TGGGCACCAA GAGCTACCTC 
CAGATGATCA ATACCGCACC TGGTGCATCC AAAACTGTTG CTGGCGACGG CAAAGGTTCT 
GTACGAAATA TCTACTTTAA ATCGGCAACC GGACTCAAGC AGTTGCCAGT GCAGATGCTA 
GGCAATCGAA TACCGGGTGG CGCCGTCATT TCGTCAACCG CATCGGCATC TTCTGGATCT 
CCTGGCTTGC GGCGAGTGGT AAACATTGGG CCCGTTTCCA AGGTGACGGC CACGTCGACA 
ACATCATCGT CGATAGCTGC TACTTTAACC CATAATAAAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725358.1
CDS	161..2965
Translation	MEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHNDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_166032.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant C (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166032.3

ORF Insert Sequence:

ATGGAGCACA GCTATATGCG AGATACGCCA GTGCGCAGCG ATGTTCACAA CGGCCTAACG 
CCCGCTAGGA ACATTCTGGT CCGCCATCCG CCACAGTGTC CCAGTTGCCA CACATATCCG 
CCGCAGCACG AGGAGCTGTC AGAGATTCAG CAGGCCCATG TGCCGCCCTA TGATGAGGTA 
GTCGCCAAGG AGGCAATGAA TGAATGCGCT CGTATTGCAA AATACGTAAA GAACAACAAT 
TCGGATGAGC AAGATTGGGT GGCGCGCGTT AATAAATTTA ATTGGACGCC CATGCAGGAG 
ATGGTTTTCG AGAAGGTCTG CTCAATTCTG GACCAGGATC AGTTGGCACG CTTGGCCAAC 
GACAAACGTC AGCACGAGCC TATCCATCGT CGCATAAGCT CGGACAAATC CGCGTCCAGG 
TTTCGCAAAC TTCTAGCCAG CGTGGCTTGG GAGTCACGCA TTACGCAGTG GATCCATGCT 
CTGCTCATGG AGCACTTGTC GCCTTCGTAT ATGGCCTCTT ATCTGGAAAT TCTTCAGACT 
CTGAAGACCA AGTTGCCCAC TTTAGTGGAC AAGATGCTGT TTGGTAGGCC CTTAAATAAT 
AGTCAGGAAC TGCTGGCACC GGTGATGAAA AAACGATGGG AACCAAATAT CATGCCCAAG 
TCCCGTCAGC TAACGCATAA CGCCATCATG GTGGTGCTGC CCAACATGCC GACAACTGGA 
ACAGTTTCGG ACCGCATGCA GAAGTGGTAC CAGGCATTAT CTACTATCAC TCAGGTGGTT 
CAGATTTCGC TGCCGAATAC AAACAACAGA ATTGGGAATC AGAATCTGGA CCATGTGGCC 
GAAACCATTG TGTCTATCAC ACGCGTCAAA ATCAACGAAT TGCGCACAGA AAACCCCAGT 
CGTGGCATCA TACTCGTTGG ATTTAATGCT GGAGCTGCCT TGGCTCTACA GGTTGCTCTA 
TCGGAGAGCG TTGCCTGTGT GGTTTGCATG GGATTTGCTT ACAACACAAT ACGTGGGCCA 
CGCGGAACCC CCGATGACCG CATGTTGGAC ATAAAAGCGC CGATACTCTT TGTCATCGGA 
CAAAATTCCG CACGCACAAG TCAGGAGGAA ATGGAAGGGT TACGCGAACG AATGCAGTCC 
GAATCTTCGT TGGTCGTAGT GGGTAGTGCA GATGATGCAC TGCGTGTGCC TAAGAGCAAG 
CGGCGAATAG AAGGCGTTAC ACAGTCGATG GTTGATTACA TGGTTGTCGA GGAAATCTTT 
GAGTTTGTGA ACAGAACGCT AAGCAATCCA CCTGGACCGC GTATGCCCAC ATGTTTAATG 
CATCAGCAGG GCTACCAGCG ACTGCCAAAG CAGTCACCTC ACATCCTGGC CGATGGAAAC 
GCAAACAAGG CTGTTCAGCA GATGCGTAAG CGAAAGATGG ACGGTGGTTT AGACGACTCA 
ATGGGTCAGC CTTCCAAAAC AAAGTTTGTT CCTCACAATG ATGCCCAGGA CTATGGAGAT 
GCCCAAACCA CTGCAGGTGG TTCAGCGAGC CTGCCAAAGG TGATAACTCC CGGTGTTTTG 
GGAAAACAAC TGCCGCCCGT CAATCTCGCA GCTGGCACCA AGATTAAGAT GATCCCATCC 
AGCCAGTTCG TTCAAATCAA ATCGCTACCG TCACAGAGCA AACTCATTAA CTACACGCTA 
AACAAACCGA GTGTCGGGAC AAGCAGCACC GCCAACATTG GCAGCGTGGT GAAGACCCTA 
CCATGCTCAT CCGTTAGTGG CCAGCAGATC ATCACCTTAA AAACGCCCTC GGGACAAACG 
CAGCAGTTTG CAACAGCAGC CACATCAACA GGTGCCGCTG GTTCATCGAC ATCAACAGGA 
ACGGGACAGA AAAAGTACAC AGTTTTTAAG AACGCCAACG GCATGACCAT GCTGCATCTC 
ACCAAGAGCG TACCCACTAC AGGTTCTAGC AGCGGCTCTG CGAATATGGA TTTGTCCAAC 
ATTATCGATA TGCCCATTGT TTTTGCCGAT AACGAAGGCA ATATTCCCGA GCACCAACAG 
GCGGATAAGG ATATTAAACC AGCCCCCATT CGAAGTGCGA ATAAGAGCCC GTTGATCATC 
AGTCAGAAAA TCATTAAGGA GGCAAATAAG CCCTCGGGCA TGGTTACCAG TGGCAGCATT 
ACTGCCGTCA GCAAGCCGGG GAATATTGTG CTTAACAAAG GCCTTCAGCA ATTGCTAAAT 
TCATCGCCCG GTGGAACACT TGCCACTCAG AATAAAGTTG TGTACCTCAA CCGGAGTACA 
ATTAAGCCAA TGGGAAATGT GGCGCCTGGT ACTCATCGTG TCCCTATAAG GATTGTGAGC 
ACCCCAAACA CCGGAGGTGC AGTGACGACC ACAGCTTCCA GTAGTCCCAT TATGGTTGAT 
CCAGCCACAG GATCCAAGGT TGCCAAGACC GTCAATGTGC AGACAATCAA GCCTACTGGT 
TCCGTTCTGG CTCAAGGCAC CATCCGTCAA GCGGGCAACG TGGGCACCAA GAGCTACCTC 
CAGATGATCA ATACCGCACC TGGTGCATCC AAAACTGTTG CTGGCGACGG CAAAGGTTCT 
GTACGAAATA TCTACTTTAA ATCGGCAACC GGACTCAAGC AGTTGCCAGT GCAGATGCTA 
GGCAATCGAA TACCGGGTGG CGCCGTCATT TCGTCAACCG CATCGGCATC TTCTGGATCT 
CCTGGCTTGC GGCGAGTGGT AAACATTGGG CCCGTTTCCA AGGTGACGGC CACGTCGACA 
ACATCATCGT CGATAGCTGC TACTTTAACC CATAATAAAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rcd1 ( NM_001274035.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260964.1 Drosophila melanogaster reduction in Cnn dots 1, transcript variant F (Rcd1), mRNA.

Price & Availability↑

CloneID	OFa09927
Clone ID Related Accession (Same CDS sequence)	NM_001274035.2
Accession Version	NM_001274035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274035.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGT TCTTCGATTG CTTCAATGAT TTTCTGCAGA GCGGCGGCAA CTTAGTGCAG 
AATGTGTCCC AGGAGCAGGA ACAGAAAATC GCGGACCAGG AACTGGACGG CGTCATAGAG 
CTAATGCCGC CAACCACCCC CACGCCCAGC GAGAGTTCCA GTACCATTTC GGCCCCTAGT 
GAATCCTCCT TTAGCGAGAA AATGGAGCAC AGCTATATGC GAGATACGCC AGTGCGCAGC 
GATGTTCACA ACGGCCTAAC GCCCGCTAGG AACATTCTGG TCCGCCATCC GCCACAGTGT 
CCCAGTTGCC ACACATATCC GCCGCAGCAC GAGGAGCTGT CAGAGATTCA GCAGGCCCAT 
GTGCCGCCCT ATGATGAGGT AGTCGCCAAG GAGGCAATGA ATGAATGCGC TCGTATTGCA 
AAATACGTAA AGAACAACAA TTCGGATGAG CAAGATTGGG TGGCGCGCGT TAATAAATTT 
AATTGGACGC CCATGCAGGA GATGGTTTTC GAGAAGGTCT GCTCAATTCT GGACCAGGAT 
CAGTTGGCAC GCTTGGCCAA CGACAAACGT CAGCACGAGC CTATCCATCG TCGCATAAGC 
TCGGACAAAT CCGCGTCCAG GTTTCGCAAA CTTCTAGCCA GCGTGGCTTG GGAGTCACGC 
ATTACGCAGT GGATCCATGC TCTGCTCATG GAGCACTTGT CGCCTTCGTA TATGGCCTCT 
TATCTGGAAA TTCTTCAGAC TCTGAAGACC AAGTTGCCCA CTTTAGTGGA CAAGATGCTG 
TTTGGTAGGC CCTTAAATAA TAGTCAGGAA CTGCTGGCAC CGGTGATGAA AAAACGATGG 
GAACCAAATA TCATGCCCAA GTCCCGTCAG CTAACGCATA ACGCCATCAT GGTGGTGCTG 
CCCAACATGC CGACAACTGG AACAGTTTCG GACCGCATGC AGAAGTGGTA CCAGGCATTA 
TCTACTATCA CTCAGGTGGT TCAGATTTCG CTGCCGAATA CAAACAACAG AATTGGGAAT 
CAGAATCTGG ACCATGTGGC CGAAACCATT GTGTCTATCA CACGCGTCAA AATCAACGAA 
TTGCGCACAG AAAACCCCAG TCGTGGCATC ATACTCGTTG GATTTAATGC TGGAGCTGCC 
TTGGCTCTAC AGGTTGCTCT ATCGGAGAGC GTTGCCTGTG TGGTTTGCAT GGGATTTGCT 
TACAACACAA TACGTGGGCC ACGCGGAACC CCCGATGACC GCATGTTGGA CATAAAAGCG 
CCGATACTCT TTGTCATCGG ACAAAATTCC GCACGCACAA GTCAGGAGGA AATGGAAGGG 
TTACGCGAAC GAATGCAGTC CGAATCTTCG TTGGTCGTAG TGGGTAGTGC AGATGATGCA 
CTGCGTGTGC CTAAGAGCAA GCGGCGAATA GAAGGCGTTA CACAGTCGAT GGTTGATTAC 
ATGGTTGTCG AGGAAATCTT TGAGTTTGTG AACAGAACGC TAAGCAATCC ACCTGGACCG 
CGTATGCCCA CATGTTTAAT GCATCAGCAG GGCTACCAGC GACTGCCAAA GCAGTCACCT 
CACATCCTGG CCGATGGAAA CGCAAACAAG GCTGTTCAGC AGATGCGTAA GCGAAAGATG 
GACGGTGGTT TAGACGACTC AATGGGTCAG CCTTCCAAAA CAAAGTTTGT TCCTCACATT 
GGAAGACCCC GAACACGCCC CCTTCCCAAT GTCGGTGGCT CGGCACGCTC TGTTAGCCAA 
AAAGGTGCAC CTCATCCCGA CCAACCAAAA CTGAGCGGCG AAGAACCTAA CCAATCCATT 
GAATTCATAC TAGATGATGC CCAGGACTAT GGAGATGCCC AAACCACTGC AGGTGGTTCA 
GCGAGCCTGC CAAAGGTGAT AACTCCCGGT GTTTTGGGAA AACAACTGCC GCCCGTCAAT 
CTCGCAGCTG GCACCAAGAT TAAGATGATC CCATCCAGCC AGTTCGTTCA AATCAAATCG 
CTACCGTCAC AGAGCAAACT CATTAACTAC ACGCTAAACA AACCGAGTGT CGGGACAAGC 
AGCACCGCCA ACATTGGCAG CGTGGTGAAG ACCCTACCAT GCTCATCCGT TAGTGGCCAG 
CAGATCATCA CCTTAAAAAC GCCCTCGGGA CAAACGCAGC AGTTTGCAAC AGCAGCCACA 
TCAACAGGTG CCGCTGGTTC ATCGACATCA ACAGGAACGG GACAGAAAAA GTACACAGTT 
TTTAAGAACG CCAACGGCAT GACCATGCTG CATCTCACCA AGAGCGTACC CACTACAGGT 
TCTAGCAGCG GCTCTGCGAA TATGGATTTG TCCAACATTA TCGATATGCC CATTGTTTTT 
GCCGATAACG AAGGCAATAT TCCCGAGCAC CAACAGGCGG ATAAGGATAT TAAACCAGCC 
CCCATTCGAA GTGCGAATAA GAGCCCGTTG ATCATCAGTC AGAAAATCAT TAAGGAGGCA 
AATAAGCCCT CGGGCATGGT TACCAGTGGC AGCATTACTG CCGTCAGCAA GCCGGGGAAT 
ATTGTGCTTA ACAAAGGCCT TCAGCAATTG CTAAATTCAT CGCCCGGTGG AACACTTGCC 
ACTCAGAATA AAGTTGTGTA CCTCAACCGG AGTACAATTA AGCCAATGGG AAATGTGGCG 
CCTGGTACTC ATCGTGTCCC TATAAGGATT GTGAGCACCC CAAACACCGG AGGTGCAGTG 
ACGACCACAG CTTCCAGTAG TCCCATTATG GTTGATCCAG CCACAGGATC CAAGGTTGCC 
AAGACCGTCA ATGTGCAGAC AATCAAGCCT ACTGGTTCCG TTCTGGCTCA AGGCACCATC 
CGTCAAGCGG GCAACGTGGG CACCAAGAGC TACCTCCAGA TGATCAATAC CGCACCTGGT 
GCATCCAAAA CTGTTGCTGG CGACGGCAAA GGTTCTGTAC GAAATATCTA CTTTAAATCG 
GCAACCGGAC TCAAGCAGTT GCCAGTGCAG ATGCTAGGCA ATCGAATACC GGGTGGCGCC 
GTCATTTCGT CAACCGCATC GGCATCTTCT GGATCTCCTG GCTTGCGGCG AGTGGTAAAC 
ATTGGGCCCG TTTCCAAGGT GACGGCCACG TCGACAACAT CATCGTCGAT AGCTGCTACT 
TTAACCCATA ATAAAATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260964.1
CDS	123..3263
Translation	MSSFFDCFNDFLQSGGNLVQNVSQEQEQKIADQELDGVIELMPPTTPTPSESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHIGRPRTRPLPNVGGSARSVSQKGAPHPDQPKLSGEEPNQSIEFILDDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_001274035.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant F (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274035.2

ORF Insert Sequence:

ATGTCTTCGT TCTTCGATTG CTTCAATGAT TTTCTGCAGA GCGGCGGCAA CTTAGTGCAG 
AATGTGTCCC AGGAGCAGGA ACAGAAAATC GCGGACCAGG AACTGGACGG CGTCATAGAG 
CTAATGCCGC CAACCACCCC CACGCCCAGC GAGAGTTCCA GTACCATTTC GGCCCCTAGT 
GAATCCTCCT TTAGCGAGAA AATGGAGCAC AGCTATATGC GAGATACGCC AGTGCGCAGC 
GATGTTCACA ACGGCCTAAC GCCCGCTAGG AACATTCTGG TCCGCCATCC GCCACAGTGT 
CCCAGTTGCC ACACATATCC GCCGCAGCAC GAGGAGCTGT CAGAGATTCA GCAGGCCCAT 
GTGCCGCCCT ATGATGAGGT AGTCGCCAAG GAGGCAATGA ATGAATGCGC TCGTATTGCA 
AAATACGTAA AGAACAACAA TTCGGATGAG CAAGATTGGG TGGCGCGCGT TAATAAATTT 
AATTGGACGC CCATGCAGGA GATGGTTTTC GAGAAGGTCT GCTCAATTCT GGACCAGGAT 
CAGTTGGCAC GCTTGGCCAA CGACAAACGT CAGCACGAGC CTATCCATCG TCGCATAAGC 
TCGGACAAAT CCGCGTCCAG GTTTCGCAAA CTTCTAGCCA GCGTGGCTTG GGAGTCACGC 
ATTACGCAGT GGATCCATGC TCTGCTCATG GAGCACTTGT CGCCTTCGTA TATGGCCTCT 
TATCTGGAAA TTCTTCAGAC TCTGAAGACC AAGTTGCCCA CTTTAGTGGA CAAGATGCTG 
TTTGGTAGGC CCTTAAATAA TAGTCAGGAA CTGCTGGCAC CGGTGATGAA AAAACGATGG 
GAACCAAATA TCATGCCCAA GTCCCGTCAG CTAACGCATA ACGCCATCAT GGTGGTGCTG 
CCCAACATGC CGACAACTGG AACAGTTTCG GACCGCATGC AGAAGTGGTA CCAGGCATTA 
TCTACTATCA CTCAGGTGGT TCAGATTTCG CTGCCGAATA CAAACAACAG AATTGGGAAT 
CAGAATCTGG ACCATGTGGC CGAAACCATT GTGTCTATCA CACGCGTCAA AATCAACGAA 
TTGCGCACAG AAAACCCCAG TCGTGGCATC ATACTCGTTG GATTTAATGC TGGAGCTGCC 
TTGGCTCTAC AGGTTGCTCT ATCGGAGAGC GTTGCCTGTG TGGTTTGCAT GGGATTTGCT 
TACAACACAA TACGTGGGCC ACGCGGAACC CCCGATGACC GCATGTTGGA CATAAAAGCG 
CCGATACTCT TTGTCATCGG ACAAAATTCC GCACGCACAA GTCAGGAGGA AATGGAAGGG 
TTACGCGAAC GAATGCAGTC CGAATCTTCG TTGGTCGTAG TGGGTAGTGC AGATGATGCA 
CTGCGTGTGC CTAAGAGCAA GCGGCGAATA GAAGGCGTTA CACAGTCGAT GGTTGATTAC 
ATGGTTGTCG AGGAAATCTT TGAGTTTGTG AACAGAACGC TAAGCAATCC ACCTGGACCG 
CGTATGCCCA CATGTTTAAT GCATCAGCAG GGCTACCAGC GACTGCCAAA GCAGTCACCT 
CACATCCTGG CCGATGGAAA CGCAAACAAG GCTGTTCAGC AGATGCGTAA GCGAAAGATG 
GACGGTGGTT TAGACGACTC AATGGGTCAG CCTTCCAAAA CAAAGTTTGT TCCTCACATT 
GGAAGACCCC GAACACGCCC CCTTCCCAAT GTCGGTGGCT CGGCACGCTC TGTTAGCCAA 
AAAGGTGCAC CTCATCCCGA CCAACCAAAA CTGAGCGGCG AAGAACCTAA CCAATCCATT 
GAATTCATAC TAGATGATGC CCAGGACTAT GGAGATGCCC AAACCACTGC AGGTGGTTCA 
GCGAGCCTGC CAAAGGTGAT AACTCCCGGT GTTTTGGGAA AACAACTGCC GCCCGTCAAT 
CTCGCAGCTG GCACCAAGAT TAAGATGATC CCATCCAGCC AGTTCGTTCA AATCAAATCG 
CTACCGTCAC AGAGCAAACT CATTAACTAC ACGCTAAACA AACCGAGTGT CGGGACAAGC 
AGCACCGCCA ACATTGGCAG CGTGGTGAAG ACCCTACCAT GCTCATCCGT TAGTGGCCAG 
CAGATCATCA CCTTAAAAAC GCCCTCGGGA CAAACGCAGC AGTTTGCAAC AGCAGCCACA 
TCAACAGGTG CCGCTGGTTC ATCGACATCA ACAGGAACGG GACAGAAAAA GTACACAGTT 
TTTAAGAACG CCAACGGCAT GACCATGCTG CATCTCACCA AGAGCGTACC CACTACAGGT 
TCTAGCAGCG GCTCTGCGAA TATGGATTTG TCCAACATTA TCGATATGCC CATTGTTTTT 
GCCGATAACG AAGGCAATAT TCCCGAGCAC CAACAGGCGG ATAAGGATAT TAAACCAGCC 
CCCATTCGAA GTGCGAATAA GAGCCCGTTG ATCATCAGTC AGAAAATCAT TAAGGAGGCA 
AATAAGCCCT CGGGCATGGT TACCAGTGGC AGCATTACTG CCGTCAGCAA GCCGGGGAAT 
ATTGTGCTTA ACAAAGGCCT TCAGCAATTG CTAAATTCAT CGCCCGGTGG AACACTTGCC 
ACTCAGAATA AAGTTGTGTA CCTCAACCGG AGTACAATTA AGCCAATGGG AAATGTGGCG 
CCTGGTACTC ATCGTGTCCC TATAAGGATT GTGAGCACCC CAAACACCGG AGGTGCAGTG 
ACGACCACAG CTTCCAGTAG TCCCATTATG GTTGATCCAG CCACAGGATC CAAGGTTGCC 
AAGACCGTCA ATGTGCAGAC AATCAAGCCT ACTGGTTCCG TTCTGGCTCA AGGCACCATC 
CGTCAAGCGG GCAACGTGGG CACCAAGAGC TACCTCCAGA TGATCAATAC CGCACCTGGT 
GCATCCAAAA CTGTTGCTGG CGACGGCAAA GGTTCTGTAC GAAATATCTA CTTTAAATCG 
GCAACCGGAC TCAAGCAGTT GCCAGTGCAG ATGCTAGGCA ATCGAATACC GGGTGGCGCC 
GTCATTTCGT CAACCGCATC GGCATCTTCT GGATCTCCTG GCTTGCGGCG AGTGGTAAAC 
ATTGGGCCCG TTTCCAAGGT GACGGCCACG TCGACAACAT CATCGTCGAT AGCTGCTACT 
TTAACCCATA ATAAAATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rcd1 ( NM_001274033.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260962.1 Drosophila melanogaster reduction in Cnn dots 1, transcript variant D (Rcd1), mRNA.

Price & Availability↑

CloneID	OFa09928
Clone ID Related Accession (Same CDS sequence)	NM_001274033.2
Accession Version	NM_001274033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274033.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGT TCTTCGATTG CTTCAATGAT TTTCTGCAGA GCGGCGGCAA CTTAGTGCAG 
AATGTGTCCC AGGAGCAGGA ACAGAAAATC GCGGACCAGG AACTGGACGG CGTCATAGAG 
CTAATGCCGC CAACCACCCC CACGCCCAGC GAGAGTTCCA GTACCATTTC GGCCCCTAGT 
GAATCCTCCT TTAGCGAGAA AATGGAGCAC AGCTATATGC GAGATACGCC AGTGCGCAGC 
GATGTTCACA ACGGCCTAAC GCCCGCTAGG AACATTCTGG TCCGCCATCC GCCACAGTGT 
CCCAGTTGCC ACACATATCC GCCGCAGCAC GAGGAGCTGT CAGAGATTCA GCAGGCCCAT 
GTGCCGCCCT ATGATGAGGT AGTCGCCAAG GAGGCAATGA ATGAATGCGC TCGTATTGCA 
AAATACGTAA AGAACAACAA TTCGGATGAG CAAGATTGGG TGGCGCGCGT TAATAAATTT 
AATTGGACGC CCATGCAGGA GATGGTTTTC GAGAAGGTCT GCTCAATTCT GGACCAGGAT 
CAGTTGGCAC GCTTGGCCAA CGACAAACGT CAGCACGAGC CTATCCATCG TCGCATAAGC 
TCGGACAAAT CCGCGTCCAG GTTTCGCAAA CTTCTAGCCA GCGTGGCTTG GGAGTCACGC 
ATTACGCAGT GGATCCATGC TCTGCTCATG GAGCACTTGT CGCCTTCGTA TATGGCCTCT 
TATCTGGAAA TTCTTCAGAC TCTGAAGACC AAGTTGCCCA CTTTAGTGGA CAAGATGCTG 
TTTGGTAGGC CCTTAAATAA TAGTCAGGAA CTGCTGGCAC CGGTGATGAA AAAACGATGG 
GAACCAAATA TCATGCCCAA GTCCCGTCAG CTAACGCATA ACGCCATCAT GGTGGTGCTG 
CCCAACATGC CGACAACTGG AACAGTTTCG GACCGCATGC AGAAGTGGTA CCAGGCATTA 
TCTACTATCA CTCAGGTGGT TCAGATTTCG CTGCCGAATA CAAACAACAG AATTGGGAAT 
CAGAATCTGG ACCATGTGGC CGAAACCATT GTGTCTATCA CACGCGTCAA AATCAACGAA 
TTGCGCACAG AAAACCCCAG TCGTGGCATC ATACTCGTTG GATTTAATGC TGGAGCTGCC 
TTGGCTCTAC AGGTTGCTCT ATCGGAGAGC GTTGCCTGTG TGGTTTGCAT GGGATTTGCT 
TACAACACAA TACGTGGGCC ACGCGGAACC CCCGATGACC GCATGTTGGA CATAAAAGCG 
CCGATACTCT TTGTCATCGG ACAAAATTCC GCACGCACAA GTCAGGAGGA AATGGAAGGG 
TTACGCGAAC GAATGCAGTC CGAATCTTCG TTGGTCGTAG TGGGTAGTGC AGATGATGCA 
CTGCGTGTGC CTAAGAGCAA GCGGCGAATA GAAGGCGTTA CACAGTCGAT GGTTGATTAC 
ATGGTTGTCG AGGAAATCTT TGAGTTTGTG AACAGAACGC TAAGCAATCC ACCTGGACCG 
CGTATGCCCA CATGTTTAAT GCATCAGCAG GGCTACCAGC GACTGCCAAA GCAGTCACCT 
CACATCCTGG CCGATGGAAA CGCAAACAAG GCTGTTCAGC AGATGCGTAA GCGAAAGATG 
GACGGTGGTT TAGACGACTC AATGGGTCAG CCTTCCAAAA CAAAGTTTGT TCCTCACAAT 
CGCGTCCACA AGCCGAAGCC ACCGCGCCTC ATTGATCCGT TTGCTGTGAA GCGAAAGGTT 
GGAAGACCCC GAACACGCCC CCTTCCCAAT GTCGGTGGCT CGGCACGCTC TGTTAGCCAA 
AAAGGTGCAC CTCATCCCGA CCAACCAAAA CTGAGCGGCG AAGAACCTAA CCAATCCATT 
GAATTCATAC TAGATGATGC CCAGGACTAT GGAGATGCCC AAACCACTGC AGGTGGTTCA 
GCGAGCCTGC CAAAGGTGAT AACTCCCGGT GTTTTGGGAA AACAACTGCC GCCCGTCAAT 
CTCGCAGCTG GCACCAAGAT TAAGATGATC CCATCCAGCC AGTTCGTTCA AATCAAATCG 
CTACCGTCAC AGAGCAAACT CATTAACTAC ACGCTAAACA AACCGAGTGT CGGGACAAGC 
AGCACCGCCA ACATTGGCAG CGTGGTGAAG ACCCTACCAT GCTCATCCGT TAGTGGCCAG 
CAGATCATCA CCTTAAAAAC GCCCTCGGGA CAAACGCAGC AGTTTGCAAC AGCAGCCACA 
TCAACAGGTG CCGCTGGTTC ATCGACATCA ACAGGAACGG GACAGAAAAA GTACACAGTT 
TTTAAGAACG CCAACGGCAT GACCATGCTG CATCTCACCA AGAGCGTACC CACTACAGGT 
TCTAGCAGCG GCTCTGCGAA TATGGATTTG TCCAACATTA TCGATATGCC CATTGTTTTT 
GCCGATAACG AAGGCAATAT TCCCGAGCAC CAACAGGCGG ATAAGGATAT TAAACCAGCC 
CCCATTCGAA GTGCGAATAA GAGCCCGTTG ATCATCAGTC AGAAAATCAT TAAGGAGGCA 
AATAAGCCCT CGGGCATGGT TACCAGTGGC AGCATTACTG CCGTCAGCAA GCCGGGGAAT 
ATTGTGCTTA ACAAAGGCCT TCAGCAATTG CTAAATTCAT CGCCCGGTGG AACACTTGCC 
ACTCAGAATA AAGTTGTGTA CCTCAACCGG AGTACAATTA AGCCAATGGG AAATGTGGCG 
CCTGGTACTC ATCGTGTCCC TATAAGGATT GTGAGCACCC CAAACACCGG AGGTGCAGTG 
ACGACCACAG CTTCCAGTAG TCCCATTATG GTTGATCCAG CCACAGGATC CAAGGTTGCC 
AAGACCGTCA ATGTGCAGAC AATCAAGCCT ACTGGTTCCG TTCTGGCTCA AGGCACCATC 
CGTCAAGCGG GCAACGTGGG CACCAAGAGC TACCTCCAGA TGATCAATAC CGCACCTGGT 
GCATCCAAAA CTGTTGCTGG CGACGGCAAA GGTTCTGTAC GAAATATCTA CTTTAAATCG 
GCAACCGGAC TCAAGCAGTT GCCAGTGCAG ATGCTAGGCA ATCGAATACC GGGTGGCGCC 
GTCATTTCGT CAACCGCATC GGCATCTTCT GGATCTCCTG GCTTGCGGCG AGTGGTAAAC 
ATTGGGCCCG TTTCCAAGGT GACGGCCACG TCGACAACAT CATCGTCGAT AGCTGCTACT 
TTAACCCATA ATAAAATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260962.1
CDS	123..3323
Translation	MSSFFDCFNDFLQSGGNLVQNVSQEQEQKIADQELDGVIELMPPTTPTPSESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHNRVHKPKPPRLIDPFAVKRKVGRPRTRPLPNVGGSARSVSQKGAPHPDQPKLSGEEPNQSIEFILDDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_001274033.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant D (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274033.2

ORF Insert Sequence:

ATGTCTTCGT TCTTCGATTG CTTCAATGAT TTTCTGCAGA GCGGCGGCAA CTTAGTGCAG 
AATGTGTCCC AGGAGCAGGA ACAGAAAATC GCGGACCAGG AACTGGACGG CGTCATAGAG 
CTAATGCCGC CAACCACCCC CACGCCCAGC GAGAGTTCCA GTACCATTTC GGCCCCTAGT 
GAATCCTCCT TTAGCGAGAA AATGGAGCAC AGCTATATGC GAGATACGCC AGTGCGCAGC 
GATGTTCACA ACGGCCTAAC GCCCGCTAGG AACATTCTGG TCCGCCATCC GCCACAGTGT 
CCCAGTTGCC ACACATATCC GCCGCAGCAC GAGGAGCTGT CAGAGATTCA GCAGGCCCAT 
GTGCCGCCCT ATGATGAGGT AGTCGCCAAG GAGGCAATGA ATGAATGCGC TCGTATTGCA 
AAATACGTAA AGAACAACAA TTCGGATGAG CAAGATTGGG TGGCGCGCGT TAATAAATTT 
AATTGGACGC CCATGCAGGA GATGGTTTTC GAGAAGGTCT GCTCAATTCT GGACCAGGAT 
CAGTTGGCAC GCTTGGCCAA CGACAAACGT CAGCACGAGC CTATCCATCG TCGCATAAGC 
TCGGACAAAT CCGCGTCCAG GTTTCGCAAA CTTCTAGCCA GCGTGGCTTG GGAGTCACGC 
ATTACGCAGT GGATCCATGC TCTGCTCATG GAGCACTTGT CGCCTTCGTA TATGGCCTCT 
TATCTGGAAA TTCTTCAGAC TCTGAAGACC AAGTTGCCCA CTTTAGTGGA CAAGATGCTG 
TTTGGTAGGC CCTTAAATAA TAGTCAGGAA CTGCTGGCAC CGGTGATGAA AAAACGATGG 
GAACCAAATA TCATGCCCAA GTCCCGTCAG CTAACGCATA ACGCCATCAT GGTGGTGCTG 
CCCAACATGC CGACAACTGG AACAGTTTCG GACCGCATGC AGAAGTGGTA CCAGGCATTA 
TCTACTATCA CTCAGGTGGT TCAGATTTCG CTGCCGAATA CAAACAACAG AATTGGGAAT 
CAGAATCTGG ACCATGTGGC CGAAACCATT GTGTCTATCA CACGCGTCAA AATCAACGAA 
TTGCGCACAG AAAACCCCAG TCGTGGCATC ATACTCGTTG GATTTAATGC TGGAGCTGCC 
TTGGCTCTAC AGGTTGCTCT ATCGGAGAGC GTTGCCTGTG TGGTTTGCAT GGGATTTGCT 
TACAACACAA TACGTGGGCC ACGCGGAACC CCCGATGACC GCATGTTGGA CATAAAAGCG 
CCGATACTCT TTGTCATCGG ACAAAATTCC GCACGCACAA GTCAGGAGGA AATGGAAGGG 
TTACGCGAAC GAATGCAGTC CGAATCTTCG TTGGTCGTAG TGGGTAGTGC AGATGATGCA 
CTGCGTGTGC CTAAGAGCAA GCGGCGAATA GAAGGCGTTA CACAGTCGAT GGTTGATTAC 
ATGGTTGTCG AGGAAATCTT TGAGTTTGTG AACAGAACGC TAAGCAATCC ACCTGGACCG 
CGTATGCCCA CATGTTTAAT GCATCAGCAG GGCTACCAGC GACTGCCAAA GCAGTCACCT 
CACATCCTGG CCGATGGAAA CGCAAACAAG GCTGTTCAGC AGATGCGTAA GCGAAAGATG 
GACGGTGGTT TAGACGACTC AATGGGTCAG CCTTCCAAAA CAAAGTTTGT TCCTCACAAT 
CGCGTCCACA AGCCGAAGCC ACCGCGCCTC ATTGATCCGT TTGCTGTGAA GCGAAAGGTT 
GGAAGACCCC GAACACGCCC CCTTCCCAAT GTCGGTGGCT CGGCACGCTC TGTTAGCCAA 
AAAGGTGCAC CTCATCCCGA CCAACCAAAA CTGAGCGGCG AAGAACCTAA CCAATCCATT 
GAATTCATAC TAGATGATGC CCAGGACTAT GGAGATGCCC AAACCACTGC AGGTGGTTCA 
GCGAGCCTGC CAAAGGTGAT AACTCCCGGT GTTTTGGGAA AACAACTGCC GCCCGTCAAT 
CTCGCAGCTG GCACCAAGAT TAAGATGATC CCATCCAGCC AGTTCGTTCA AATCAAATCG 
CTACCGTCAC AGAGCAAACT CATTAACTAC ACGCTAAACA AACCGAGTGT CGGGACAAGC 
AGCACCGCCA ACATTGGCAG CGTGGTGAAG ACCCTACCAT GCTCATCCGT TAGTGGCCAG 
CAGATCATCA CCTTAAAAAC GCCCTCGGGA CAAACGCAGC AGTTTGCAAC AGCAGCCACA 
TCAACAGGTG CCGCTGGTTC ATCGACATCA ACAGGAACGG GACAGAAAAA GTACACAGTT 
TTTAAGAACG CCAACGGCAT GACCATGCTG CATCTCACCA AGAGCGTACC CACTACAGGT 
TCTAGCAGCG GCTCTGCGAA TATGGATTTG TCCAACATTA TCGATATGCC CATTGTTTTT 
GCCGATAACG AAGGCAATAT TCCCGAGCAC CAACAGGCGG ATAAGGATAT TAAACCAGCC 
CCCATTCGAA GTGCGAATAA GAGCCCGTTG ATCATCAGTC AGAAAATCAT TAAGGAGGCA 
AATAAGCCCT CGGGCATGGT TACCAGTGGC AGCATTACTG CCGTCAGCAA GCCGGGGAAT 
ATTGTGCTTA ACAAAGGCCT TCAGCAATTG CTAAATTCAT CGCCCGGTGG AACACTTGCC 
ACTCAGAATA AAGTTGTGTA CCTCAACCGG AGTACAATTA AGCCAATGGG AAATGTGGCG 
CCTGGTACTC ATCGTGTCCC TATAAGGATT GTGAGCACCC CAAACACCGG AGGTGCAGTG 
ACGACCACAG CTTCCAGTAG TCCCATTATG GTTGATCCAG CCACAGGATC CAAGGTTGCC 
AAGACCGTCA ATGTGCAGAC AATCAAGCCT ACTGGTTCCG TTCTGGCTCA AGGCACCATC 
CGTCAAGCGG GCAACGTGGG CACCAAGAGC TACCTCCAGA TGATCAATAC CGCACCTGGT 
GCATCCAAAA CTGTTGCTGG CGACGGCAAA GGTTCTGTAC GAAATATCTA CTTTAAATCG 
GCAACCGGAC TCAAGCAGTT GCCAGTGCAG ATGCTAGGCA ATCGAATACC GGGTGGCGCC 
GTCATTTCGT CAACCGCATC GGCATCTTCT GGATCTCCTG GCTTGCGGCG AGTGGTAAAC 
ATTGGGCCCG TTTCCAAGGT GACGGCCACG TCGACAACAT CATCGTCGAT AGCTGCTACT 
TTAACCCATA ATAAAATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rcd1 ( NM_001274036.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260965.1 Drosophila melanogaster reduction in Cnn dots 1, transcript variant G (Rcd1), mRNA.

Price & Availability↑

CloneID	OFa09929
Clone ID Related Accession (Same CDS sequence)	NM_001274036.2
Accession Version	NM_001274036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274036.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACATTGGAAG ACCCCGAACA CGCCCCCTTC CCAATGTCGG TGGCTCGGCA 
CGCTCTGTTA GCCAAAAAGG TGCACCTCAT CCCGACCAAC CAAAACTGAG CGGCGAAGAA 
CCTAACCAAT CCATTGAATT CATACTAGAT GATGCCCAGG ACTATGGAGA TGCCCAAACC 
ACTGCAGGTG GTTCAGCGAG CCTGCCAAAG GTGATAACTC CCGGTGTTTT GGGAAAACAA 
CTGCCGCCCG TCAATCTCGC AGCTGGCACC AAGATTAAGA TGATCCCATC CAGCCAGTTC 
GTTCAAATCA AATCGCTACC GTCACAGAGC AAACTCATTA ACTACACGCT AAACAAACCG 
AGTGTCGGGA CAAGCAGCAC CGCCAACATT GGCAGCGTGG TGAAGACCCT ACCATGCTCA 
TCCGTTAGTG GCCAGCAGAT CATCACCTTA AAAACGCCCT CGGGACAAAC GCAGCAGTTT 
GCAACAGCAG CCACATCAAC AGGTGCCGCT GGTTCATCGA CATCAACAGG AACGGGACAG 
AAAAAGTACA CAGTTTTTAA GAACGCCAAC GGCATGACCA TGCTGCATCT CACCAAGAGC 
GTACCCACTA CAGGTTCTAG CAGCGGCTCT GCGAATATGG ATTTGTCCAA CATTATCGAT 
ATGCCCATTG TTTTTGCCGA TAACGAAGGC AATATTCCCG AGCACCAACA GGCGGATAAG 
GATATTAAAC CAGCCCCCAT TCGAAGTGCG AATAAGAGCC CGTTGATCAT CAGTCAGAAA 
ATCATTAAGG AGGCAAATAA GCCCTCGGGC ATGGTTACCA GTGGCAGCAT TACTGCCGTC 
AGCAAGCCGG GGAATATTGT GCTTAACAAA GGCCTTCAGC AATTGCTAAA TTCATCGCCC 
GGTGGAACAC TTGCCACTCA GAATAAAGTT GTGTACCTCA ACCGGAGTAC AATTAAGCCA 
ATGGGAAATG TGGCGCCTGG TACTCATCGT GTCCCTATAA GGATTGTGAG CACCCCAAAC 
ACCGGAGGTG CAGTGACGAC CACAGCTTCC AGTAGTCCCA TTATGGTTGA TCCAGCCACA 
GGATCCAAGG TTGCCAAGAC CGTCAATGTG CAGACAATCA AGCCTACTGG TTCCGTTCTG 
GCTCAAGGCA CCATCCGTCA AGCGGGCAAC GTGGGCACCA AGAGCTACCT CCAGATGATC 
AATACCGCAC CTGGTGCATC CAAAACTGTT GCTGGCGACG GCAAAGGTTC TGTACGAAAT 
ATCTACTTTA AATCGGCAAC CGGACTCAAG CAGTTGCCAG TGCAGATGCT AGGCAATCGA 
ATACCGGGTG GCGCCGTCAT TTCGTCAACC GCATCGGCAT CTTCTGGATC TCCTGGCTTG 
CGGCGAGTGG TAAACATTGG GCCCGTTTCC AAGGTGACGG CCACGTCGAC AACATCATCG 
TCGATAGCTG CTACTTTAAC CCATAATAAA ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260965.1
CDS	295..3630
Translation	MKESGSVSKNQPQTSEARGSIITYSPSKFLTSVGTGRTTYSTHTHTTTATSAATTATKTNNLPMGGSSAAYYVVKAGSLGSAASMSGCIKVSSPPKGPPMAKSPALVVTSPCTPTESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHIGRPRTRPLPNVGGSARSVSQKGAPHPDQPKLSGEEPNQSIEFILDDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_001274036.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant G (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274036.2

ORF Insert Sequence:

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACATTGGAAG ACCCCGAACA CGCCCCCTTC CCAATGTCGG TGGCTCGGCA 
CGCTCTGTTA GCCAAAAAGG TGCACCTCAT CCCGACCAAC CAAAACTGAG CGGCGAAGAA 
CCTAACCAAT CCATTGAATT CATACTAGAT GATGCCCAGG ACTATGGAGA TGCCCAAACC 
ACTGCAGGTG GTTCAGCGAG CCTGCCAAAG GTGATAACTC CCGGTGTTTT GGGAAAACAA 
CTGCCGCCCG TCAATCTCGC AGCTGGCACC AAGATTAAGA TGATCCCATC CAGCCAGTTC 
GTTCAAATCA AATCGCTACC GTCACAGAGC AAACTCATTA ACTACACGCT AAACAAACCG 
AGTGTCGGGA CAAGCAGCAC CGCCAACATT GGCAGCGTGG TGAAGACCCT ACCATGCTCA 
TCCGTTAGTG GCCAGCAGAT CATCACCTTA AAAACGCCCT CGGGACAAAC GCAGCAGTTT 
GCAACAGCAG CCACATCAAC AGGTGCCGCT GGTTCATCGA CATCAACAGG AACGGGACAG 
AAAAAGTACA CAGTTTTTAA GAACGCCAAC GGCATGACCA TGCTGCATCT CACCAAGAGC 
GTACCCACTA CAGGTTCTAG CAGCGGCTCT GCGAATATGG ATTTGTCCAA CATTATCGAT 
ATGCCCATTG TTTTTGCCGA TAACGAAGGC AATATTCCCG AGCACCAACA GGCGGATAAG 
GATATTAAAC CAGCCCCCAT TCGAAGTGCG AATAAGAGCC CGTTGATCAT CAGTCAGAAA 
ATCATTAAGG AGGCAAATAA GCCCTCGGGC ATGGTTACCA GTGGCAGCAT TACTGCCGTC 
AGCAAGCCGG GGAATATTGT GCTTAACAAA GGCCTTCAGC AATTGCTAAA TTCATCGCCC 
GGTGGAACAC TTGCCACTCA GAATAAAGTT GTGTACCTCA ACCGGAGTAC AATTAAGCCA 
ATGGGAAATG TGGCGCCTGG TACTCATCGT GTCCCTATAA GGATTGTGAG CACCCCAAAC 
ACCGGAGGTG CAGTGACGAC CACAGCTTCC AGTAGTCCCA TTATGGTTGA TCCAGCCACA 
GGATCCAAGG TTGCCAAGAC CGTCAATGTG CAGACAATCA AGCCTACTGG TTCCGTTCTG 
GCTCAAGGCA CCATCCGTCA AGCGGGCAAC GTGGGCACCA AGAGCTACCT CCAGATGATC 
AATACCGCAC CTGGTGCATC CAAAACTGTT GCTGGCGACG GCAAAGGTTC TGTACGAAAT 
ATCTACTTTA AATCGGCAAC CGGACTCAAG CAGTTGCCAG TGCAGATGCT AGGCAATCGA 
ATACCGGGTG GCGCCGTCAT TTCGTCAACC GCATCGGCAT CTTCTGGATC TCCTGGCTTG 
CGGCGAGTGG TAAACATTGG GCCCGTTTCC AAGGTGACGG CCACGTCGAC AACATCATCG 
TCGATAGCTG CTACTTTAAC CCATAATAAA ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rcd1 ( NM_001274034.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260963.1 Drosophila melanogaster reduction in Cnn dots 1, transcript variant E (Rcd1), mRNA.

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CloneID	OFa09930
Clone ID Related Accession (Same CDS sequence)	NM_001274034.2
Accession Version	NM_001274034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274034.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACAATCGCGT CCACAAGCCG AAGCCACCGC GCCTCATTGA TCCGTTTGCT 
GTGAAGCGAA AGGTTGGAAG ACCCCGAACA CGCCCCCTTC CCAATGTCGG TGGCTCGGCA 
CGCTCTGTTA GCCAAAAAGG TGCACCTCAT CCCGACCAAC CAAAACTGAG CGGCGAAGAA 
CCTAACCAAT CCATTGAATT CATACTAGAT GATGCCCAGG ACTATGGAGA TGCCCAAACC 
ACTGCAGGTG GTTCAGCGAG CCTGCCAAAG GTGATAACTC CCGGTGTTTT GGGAAAACAA 
CTGCCGCCCG TCAATCTCGC AGCTGGCACC AAGATTAAGA TGATCCCATC CAGCCAGTTC 
GTTCAAATCA AATCGCTACC GTCACAGAGC AAACTCATTA ACTACACGCT AAACAAACCG 
AGTGTCGGGA CAAGCAGCAC CGCCAACATT GGCAGCGTGG TGAAGACCCT ACCATGCTCA 
TCCGTTAGTG GCCAGCAGAT CATCACCTTA AAAACGCCCT CGGGACAAAC GCAGCAGTTT 
GCAACAGCAG CCACATCAAC AGGTGCCGCT GGTTCATCGA CATCAACAGG AACGGGACAG 
AAAAAGTACA CAGTTTTTAA GAACGCCAAC GGCATGACCA TGCTGCATCT CACCAAGAGC 
GTACCCACTA CAGGTTCTAG CAGCGGCTCT GCGAATATGG ATTTGTCCAA CATTATCGAT 
ATGCCCATTG TTTTTGCCGA TAACGAAGGC AATATTCCCG AGCACCAACA GGCGGATAAG 
GATATTAAAC CAGCCCCCAT TCGAAGTGCG AATAAGAGCC CGTTGATCAT CAGTCAGAAA 
ATCATTAAGG AGGCAAATAA GCCCTCGGGC ATGGTTACCA GTGGCAGCAT TACTGCCGTC 
AGCAAGCCGG GGAATATTGT GCTTAACAAA GGCCTTCAGC AATTGCTAAA TTCATCGCCC 
GGTGGAACAC TTGCCACTCA GAATAAAGTT GTGTACCTCA ACCGGAGTAC AATTAAGCCA 
ATGGGAAATG TGGCGCCTGG TACTCATCGT GTCCCTATAA GGATTGTGAG CACCCCAAAC 
ACCGGAGGTG CAGTGACGAC CACAGCTTCC AGTAGTCCCA TTATGGTTGA TCCAGCCACA 
GGATCCAAGG TTGCCAAGAC CGTCAATGTG CAGACAATCA AGCCTACTGG TTCCGTTCTG 
GCTCAAGGCA CCATCCGTCA AGCGGGCAAC GTGGGCACCA AGAGCTACCT CCAGATGATC 
AATACCGCAC CTGGTGCATC CAAAACTGTT GCTGGCGACG GCAAAGGTTC TGTACGAAAT 
ATCTACTTTA AATCGGCAAC CGGACTCAAG CAGTTGCCAG TGCAGATGCT AGGCAATCGA 
ATACCGGGTG GCGCCGTCAT TTCGTCAACC GCATCGGCAT CTTCTGGATC TCCTGGCTTG 
CGGCGAGTGG TAAACATTGG GCCCGTTTCC AAGGTGACGG CCACGTCGAC AACATCATCG 
TCGATAGCTG CTACTTTAAC CCATAATAAA ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260963.1
CDS	295..3690
Translation	MKESGSVSKNQPQTSEARGSIITYSPSKFLTSVGTGRTTYSTHTHTTTATSAATTATKTNNLPMGGSSAAYYVVKAGSLGSAASMSGCIKVSSPPKGPPMAKSPALVVTSPCTPTESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHNRVHKPKPPRLIDPFAVKRKVGRPRTRPLPNVGGSARSVSQKGAPHPDQPKLSGEEPNQSIEFILDDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_001274034.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant E (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274034.2

ORF Insert Sequence:

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACAATCGCGT CCACAAGCCG AAGCCACCGC GCCTCATTGA TCCGTTTGCT 
GTGAAGCGAA AGGTTGGAAG ACCCCGAACA CGCCCCCTTC CCAATGTCGG TGGCTCGGCA 
CGCTCTGTTA GCCAAAAAGG TGCACCTCAT CCCGACCAAC CAAAACTGAG CGGCGAAGAA 
CCTAACCAAT CCATTGAATT CATACTAGAT GATGCCCAGG ACTATGGAGA TGCCCAAACC 
ACTGCAGGTG GTTCAGCGAG CCTGCCAAAG GTGATAACTC CCGGTGTTTT GGGAAAACAA 
CTGCCGCCCG TCAATCTCGC AGCTGGCACC AAGATTAAGA TGATCCCATC CAGCCAGTTC 
GTTCAAATCA AATCGCTACC GTCACAGAGC AAACTCATTA ACTACACGCT AAACAAACCG 
AGTGTCGGGA CAAGCAGCAC CGCCAACATT GGCAGCGTGG TGAAGACCCT ACCATGCTCA 
TCCGTTAGTG GCCAGCAGAT CATCACCTTA AAAACGCCCT CGGGACAAAC GCAGCAGTTT 
GCAACAGCAG CCACATCAAC AGGTGCCGCT GGTTCATCGA CATCAACAGG AACGGGACAG 
AAAAAGTACA CAGTTTTTAA GAACGCCAAC GGCATGACCA TGCTGCATCT CACCAAGAGC 
GTACCCACTA CAGGTTCTAG CAGCGGCTCT GCGAATATGG ATTTGTCCAA CATTATCGAT 
ATGCCCATTG TTTTTGCCGA TAACGAAGGC AATATTCCCG AGCACCAACA GGCGGATAAG 
GATATTAAAC CAGCCCCCAT TCGAAGTGCG AATAAGAGCC CGTTGATCAT CAGTCAGAAA 
ATCATTAAGG AGGCAAATAA GCCCTCGGGC ATGGTTACCA GTGGCAGCAT TACTGCCGTC 
AGCAAGCCGG GGAATATTGT GCTTAACAAA GGCCTTCAGC AATTGCTAAA TTCATCGCCC 
GGTGGAACAC TTGCCACTCA GAATAAAGTT GTGTACCTCA ACCGGAGTAC AATTAAGCCA 
ATGGGAAATG TGGCGCCTGG TACTCATCGT GTCCCTATAA GGATTGTGAG CACCCCAAAC 
ACCGGAGGTG CAGTGACGAC CACAGCTTCC AGTAGTCCCA TTATGGTTGA TCCAGCCACA 
GGATCCAAGG TTGCCAAGAC CGTCAATGTG CAGACAATCA AGCCTACTGG TTCCGTTCTG 
GCTCAAGGCA CCATCCGTCA AGCGGGCAAC GTGGGCACCA AGAGCTACCT CCAGATGATC 
AATACCGCAC CTGGTGCATC CAAAACTGTT GCTGGCGACG GCAAAGGTTC TGTACGAAAT 
ATCTACTTTA AATCGGCAAC CGGACTCAAG CAGTTGCCAG TGCAGATGCT AGGCAATCGA 
ATACCGGGTG GCGCCGTCAT TTCGTCAACC GCATCGGCAT CTTCTGGATC TCCTGGCTTG 
CGGCGAGTGG TAAACATTGG GCCCGTTTCC AAGGTGACGG CCACGTCGAC AACATCATCG 
TCGATAGCTG CTACTTTAAC CCATAATAAA ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rcd1 ( NM_166031.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725357.2 Drosophila melanogaster reduction in Cnn dots 1, transcript variant A (Rcd1), mRNA.

Price & Availability↑

CloneID	OFa12535
Clone ID Related Accession (Same CDS sequence)	NM_166031.3
Accession Version	NM_166031.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166031.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGT TCTTCGATTG CTTCAATGAT TTTCTGCAGA GCGGCGGCAA CTTAGTGCAG 
AATGTGTCCC AGGAGCAGGA ACAGAAAATC GCGGACCAGG AACTGGACGG CGTCATAGAG 
CTAATGCCGC CAACCACCCC CACGCCCAGC GAGAGTTCCA GTACCATTTC GGCCCCTAGT 
GAATCCTCCT TTAGCGAGAA AATGGAGCAC AGCTATATGC GAGATACGCC AGTGCGCAGC 
GATGTTCACA ACGGCCTAAC GCCCGCTAGG AACATTCTGG TCCGCCATCC GCCACAGTGT 
CCCAGTTGCC ACACATATCC GCCGCAGCAC GAGGAGCTGT CAGAGATTCA GCAGGCCCAT 
GTGCCGCCCT ATGATGAGGT AGTCGCCAAG GAGGCAATGA ATGAATGCGC TCGTATTGCA 
AAATACGTAA AGAACAACAA TTCGGATGAG CAAGATTGGG TGGCGCGCGT TAATAAATTT 
AATTGGACGC CCATGCAGGA GATGGTTTTC GAGAAGGTCT GCTCAATTCT GGACCAGGAT 
CAGTTGGCAC GCTTGGCCAA CGACAAACGT CAGCACGAGC CTATCCATCG TCGCATAAGC 
TCGGACAAAT CCGCGTCCAG GTTTCGCAAA CTTCTAGCCA GCGTGGCTTG GGAGTCACGC 
ATTACGCAGT GGATCCATGC TCTGCTCATG GAGCACTTGT CGCCTTCGTA TATGGCCTCT 
TATCTGGAAA TTCTTCAGAC TCTGAAGACC AAGTTGCCCA CTTTAGTGGA CAAGATGCTG 
TTTGGTAGGC CCTTAAATAA TAGTCAGGAA CTGCTGGCAC CGGTGATGAA AAAACGATGG 
GAACCAAATA TCATGCCCAA GTCCCGTCAG CTAACGCATA ACGCCATCAT GGTGGTGCTG 
CCCAACATGC CGACAACTGG AACAGTTTCG GACCGCATGC AGAAGTGGTA CCAGGCATTA 
TCTACTATCA CTCAGGTGGT TCAGATTTCG CTGCCGAATA CAAACAACAG AATTGGGAAT 
CAGAATCTGG ACCATGTGGC CGAAACCATT GTGTCTATCA CACGCGTCAA AATCAACGAA 
TTGCGCACAG AAAACCCCAG TCGTGGCATC ATACTCGTTG GATTTAATGC TGGAGCTGCC 
TTGGCTCTAC AGGTTGCTCT ATCGGAGAGC GTTGCCTGTG TGGTTTGCAT GGGATTTGCT 
TACAACACAA TACGTGGGCC ACGCGGAACC CCCGATGACC GCATGTTGGA CATAAAAGCG 
CCGATACTCT TTGTCATCGG ACAAAATTCC GCACGCACAA GTCAGGAGGA AATGGAAGGG 
TTACGCGAAC GAATGCAGTC CGAATCTTCG TTGGTCGTAG TGGGTAGTGC AGATGATGCA 
CTGCGTGTGC CTAAGAGCAA GCGGCGAATA GAAGGCGTTA CACAGTCGAT GGTTGATTAC 
ATGGTTGTCG AGGAAATCTT TGAGTTTGTG AACAGAACGC TAAGCAATCC ACCTGGACCG 
CGTATGCCCA CATGTTTAAT GCATCAGCAG GGCTACCAGC GACTGCCAAA GCAGTCACCT 
CACATCCTGG CCGATGGAAA CGCAAACAAG GCTGTTCAGC AGATGCGTAA GCGAAAGATG 
GACGGTGGTT TAGACGACTC AATGGGTCAG CCTTCCAAAA CAAAGTTTGT TCCTCACAAT 
GATGCCCAGG ACTATGGAGA TGCCCAAACC ACTGCAGGTG GTTCAGCGAG CCTGCCAAAG 
GTGATAACTC CCGGTGTTTT GGGAAAACAA CTGCCGCCCG TCAATCTCGC AGCTGGCACC 
AAGATTAAGA TGATCCCATC CAGCCAGTTC GTTCAAATCA AATCGCTACC GTCACAGAGC 
AAACTCATTA ACTACACGCT AAACAAACCG AGTGTCGGGA CAAGCAGCAC CGCCAACATT 
GGCAGCGTGG TGAAGACCCT ACCATGCTCA TCCGTTAGTG GCCAGCAGAT CATCACCTTA 
AAAACGCCCT CGGGACAAAC GCAGCAGTTT GCAACAGCAG CCACATCAAC AGGTGCCGCT 
GGTTCATCGA CATCAACAGG AACGGGACAG AAAAAGTACA CAGTTTTTAA GAACGCCAAC 
GGCATGACCA TGCTGCATCT CACCAAGAGC GTACCCACTA CAGGTTCTAG CAGCGGCTCT 
GCGAATATGG ATTTGTCCAA CATTATCGAT ATGCCCATTG TTTTTGCCGA TAACGAAGGC 
AATATTCCCG AGCACCAACA GGCGGATAAG GATATTAAAC CAGCCCCCAT TCGAAGTGCG 
AATAAGAGCC CGTTGATCAT CAGTCAGAAA ATCATTAAGG AGGCAAATAA GCCCTCGGGC 
ATGGTTACCA GTGGCAGCAT TACTGCCGTC AGCAAGCCGG GGAATATTGT GCTTAACAAA 
GGCCTTCAGC AATTGCTAAA TTCATCGCCC GGTGGAACAC TTGCCACTCA GAATAAAGTT 
GTGTACCTCA ACCGGAGTAC AATTAAGCCA ATGGGAAATG TGGCGCCTGG TACTCATCGT 
GTCCCTATAA GGATTGTGAG CACCCCAAAC ACCGGAGGTG CAGTGACGAC CACAGCTTCC 
AGTAGTCCCA TTATGGTTGA TCCAGCCACA GGATCCAAGG TTGCCAAGAC CGTCAATGTG 
CAGACAATCA AGCCTACTGG TTCCGTTCTG GCTCAAGGCA CCATCCGTCA AGCGGGCAAC 
GTGGGCACCA AGAGCTACCT CCAGATGATC AATACCGCAC CTGGTGCATC CAAAACTGTT 
GCTGGCGACG GCAAAGGTTC TGTACGAAAT ATCTACTTTA AATCGGCAAC CGGACTCAAG 
CAGTTGCCAG TGCAGATGCT AGGCAATCGA ATACCGGGTG GCGCCGTCAT TTCGTCAACC 
GCATCGGCAT CTTCTGGATC TCCTGGCTTG CGGCGAGTGG TAAACATTGG GCCCGTTTCC 
AAGGTGACGG CCACGTCGAC AACATCATCG TCGATAGCTG CTACTTTAAC CCATAATAAA 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725357.2
CDS	123..3128
Translation	MSSFFDCFNDFLQSGGNLVQNVSQEQEQKIADQELDGVIELMPPTTPTPSESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHNDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_166031.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant A (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166031.3

ORF Insert Sequence:

ATGTCTTCGT TCTTCGATTG CTTCAATGAT TTTCTGCAGA GCGGCGGCAA CTTAGTGCAG 
AATGTGTCCC AGGAGCAGGA ACAGAAAATC GCGGACCAGG AACTGGACGG CGTCATAGAG 
CTAATGCCGC CAACCACCCC CACGCCCAGC GAGAGTTCCA GTACCATTTC GGCCCCTAGT 
GAATCCTCCT TTAGCGAGAA AATGGAGCAC AGCTATATGC GAGATACGCC AGTGCGCAGC 
GATGTTCACA ACGGCCTAAC GCCCGCTAGG AACATTCTGG TCCGCCATCC GCCACAGTGT 
CCCAGTTGCC ACACATATCC GCCGCAGCAC GAGGAGCTGT CAGAGATTCA GCAGGCCCAT 
GTGCCGCCCT ATGATGAGGT AGTCGCCAAG GAGGCAATGA ATGAATGCGC TCGTATTGCA 
AAATACGTAA AGAACAACAA TTCGGATGAG CAAGATTGGG TGGCGCGCGT TAATAAATTT 
AATTGGACGC CCATGCAGGA GATGGTTTTC GAGAAGGTCT GCTCAATTCT GGACCAGGAT 
CAGTTGGCAC GCTTGGCCAA CGACAAACGT CAGCACGAGC CTATCCATCG TCGCATAAGC 
TCGGACAAAT CCGCGTCCAG GTTTCGCAAA CTTCTAGCCA GCGTGGCTTG GGAGTCACGC 
ATTACGCAGT GGATCCATGC TCTGCTCATG GAGCACTTGT CGCCTTCGTA TATGGCCTCT 
TATCTGGAAA TTCTTCAGAC TCTGAAGACC AAGTTGCCCA CTTTAGTGGA CAAGATGCTG 
TTTGGTAGGC CCTTAAATAA TAGTCAGGAA CTGCTGGCAC CGGTGATGAA AAAACGATGG 
GAACCAAATA TCATGCCCAA GTCCCGTCAG CTAACGCATA ACGCCATCAT GGTGGTGCTG 
CCCAACATGC CGACAACTGG AACAGTTTCG GACCGCATGC AGAAGTGGTA CCAGGCATTA 
TCTACTATCA CTCAGGTGGT TCAGATTTCG CTGCCGAATA CAAACAACAG AATTGGGAAT 
CAGAATCTGG ACCATGTGGC CGAAACCATT GTGTCTATCA CACGCGTCAA AATCAACGAA 
TTGCGCACAG AAAACCCCAG TCGTGGCATC ATACTCGTTG GATTTAATGC TGGAGCTGCC 
TTGGCTCTAC AGGTTGCTCT ATCGGAGAGC GTTGCCTGTG TGGTTTGCAT GGGATTTGCT 
TACAACACAA TACGTGGGCC ACGCGGAACC CCCGATGACC GCATGTTGGA CATAAAAGCG 
CCGATACTCT TTGTCATCGG ACAAAATTCC GCACGCACAA GTCAGGAGGA AATGGAAGGG 
TTACGCGAAC GAATGCAGTC CGAATCTTCG TTGGTCGTAG TGGGTAGTGC AGATGATGCA 
CTGCGTGTGC CTAAGAGCAA GCGGCGAATA GAAGGCGTTA CACAGTCGAT GGTTGATTAC 
ATGGTTGTCG AGGAAATCTT TGAGTTTGTG AACAGAACGC TAAGCAATCC ACCTGGACCG 
CGTATGCCCA CATGTTTAAT GCATCAGCAG GGCTACCAGC GACTGCCAAA GCAGTCACCT 
CACATCCTGG CCGATGGAAA CGCAAACAAG GCTGTTCAGC AGATGCGTAA GCGAAAGATG 
GACGGTGGTT TAGACGACTC AATGGGTCAG CCTTCCAAAA CAAAGTTTGT TCCTCACAAT 
GATGCCCAGG ACTATGGAGA TGCCCAAACC ACTGCAGGTG GTTCAGCGAG CCTGCCAAAG 
GTGATAACTC CCGGTGTTTT GGGAAAACAA CTGCCGCCCG TCAATCTCGC AGCTGGCACC 
AAGATTAAGA TGATCCCATC CAGCCAGTTC GTTCAAATCA AATCGCTACC GTCACAGAGC 
AAACTCATTA ACTACACGCT AAACAAACCG AGTGTCGGGA CAAGCAGCAC CGCCAACATT 
GGCAGCGTGG TGAAGACCCT ACCATGCTCA TCCGTTAGTG GCCAGCAGAT CATCACCTTA 
AAAACGCCCT CGGGACAAAC GCAGCAGTTT GCAACAGCAG CCACATCAAC AGGTGCCGCT 
GGTTCATCGA CATCAACAGG AACGGGACAG AAAAAGTACA CAGTTTTTAA GAACGCCAAC 
GGCATGACCA TGCTGCATCT CACCAAGAGC GTACCCACTA CAGGTTCTAG CAGCGGCTCT 
GCGAATATGG ATTTGTCCAA CATTATCGAT ATGCCCATTG TTTTTGCCGA TAACGAAGGC 
AATATTCCCG AGCACCAACA GGCGGATAAG GATATTAAAC CAGCCCCCAT TCGAAGTGCG 
AATAAGAGCC CGTTGATCAT CAGTCAGAAA ATCATTAAGG AGGCAAATAA GCCCTCGGGC 
ATGGTTACCA GTGGCAGCAT TACTGCCGTC AGCAAGCCGG GGAATATTGT GCTTAACAAA 
GGCCTTCAGC AATTGCTAAA TTCATCGCCC GGTGGAACAC TTGCCACTCA GAATAAAGTT 
GTGTACCTCA ACCGGAGTAC AATTAAGCCA ATGGGAAATG TGGCGCCTGG TACTCATCGT 
GTCCCTATAA GGATTGTGAG CACCCCAAAC ACCGGAGGTG CAGTGACGAC CACAGCTTCC 
AGTAGTCCCA TTATGGTTGA TCCAGCCACA GGATCCAAGG TTGCCAAGAC CGTCAATGTG 
CAGACAATCA AGCCTACTGG TTCCGTTCTG GCTCAAGGCA CCATCCGTCA AGCGGGCAAC 
GTGGGCACCA AGAGCTACCT CCAGATGATC AATACCGCAC CTGGTGCATC CAAAACTGTT 
GCTGGCGACG GCAAAGGTTC TGTACGAAAT ATCTACTTTA AATCGGCAAC CGGACTCAAG 
CAGTTGCCAG TGCAGATGCT AGGCAATCGA ATACCGGGTG GCGCCGTCAT TTCGTCAACC 
GCATCGGCAT CTTCTGGATC TCCTGGCTTG CGGCGAGTGG TAAACATTGG GCCCGTTTCC 
AAGGTGACGG CCACGTCGAC AACATCATCG TCGATAGCTG CTACTTTAAC CCATAATAAA 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rcd1 ( NM_137083.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610927.3 Drosophila melanogaster reduction in Cnn dots 1, transcript variant B (Rcd1), mRNA.

Price & Availability↑

CloneID	OFa12538
Clone ID Related Accession (Same CDS sequence)	NM_137083.4
Accession Version	NM_137083.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137083.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACAATGATGC CCAGGACTAT GGAGATGCCC AAACCACTGC AGGTGGTTCA 
GCGAGCCTGC CAAAGGTGAT AACTCCCGGT GTTTTGGGAA AACAACTGCC GCCCGTCAAT 
CTCGCAGCTG GCACCAAGAT TAAGATGATC CCATCCAGCC AGTTCGTTCA AATCAAATCG 
CTACCGTCAC AGAGCAAACT CATTAACTAC ACGCTAAACA AACCGAGTGT CGGGACAAGC 
AGCACCGCCA ACATTGGCAG CGTGGTGAAG ACCCTACCAT GCTCATCCGT TAGTGGCCAG 
CAGATCATCA CCTTAAAAAC GCCCTCGGGA CAAACGCAGC AGTTTGCAAC AGCAGCCACA 
TCAACAGGTG CCGCTGGTTC ATCGACATCA ACAGGAACGG GACAGAAAAA GTACACAGTT 
TTTAAGAACG CCAACGGCAT GACCATGCTG CATCTCACCA AGAGCGTACC CACTACAGGT 
TCTAGCAGCG GCTCTGCGAA TATGGATTTG TCCAACATTA TCGATATGCC CATTGTTTTT 
GCCGATAACG AAGGCAATAT TCCCGAGCAC CAACAGGCGG ATAAGGATAT TAAACCAGCC 
CCCATTCGAA GTGCGAATAA GAGCCCGTTG ATCATCAGTC AGAAAATCAT TAAGGAGGCA 
AATAAGCCCT CGGGCATGGT TACCAGTGGC AGCATTACTG CCGTCAGCAA GCCGGGGAAT 
ATTGTGCTTA ACAAAGGCCT TCAGCAATTG CTAAATTCAT CGCCCGGTGG AACACTTGCC 
ACTCAGAATA AAGTTGTGTA CCTCAACCGG AGTACAATTA AGCCAATGGG AAATGTGGCG 
CCTGGTACTC ATCGTGTCCC TATAAGGATT GTGAGCACCC CAAACACCGG AGGTGCAGTG 
ACGACCACAG CTTCCAGTAG TCCCATTATG GTTGATCCAG CCACAGGATC CAAGGTTGCC 
AAGACCGTCA ATGTGCAGAC AATCAAGCCT ACTGGTTCCG TTCTGGCTCA AGGCACCATC 
CGTCAAGCGG GCAACGTGGG CACCAAGAGC TACCTCCAGA TGATCAATAC CGCACCTGGT 
GCATCCAAAA CTGTTGCTGG CGACGGCAAA GGTTCTGTAC GAAATATCTA CTTTAAATCG 
GCAACCGGAC TCAAGCAGTT GCCAGTGCAG ATGCTAGGCA ATCGAATACC GGGTGGCGCC 
GTCATTTCGT CAACCGCATC GGCATCTTCT GGATCTCCTG GCTTGCGGCG AGTGGTAAAC 
ATTGGGCCCG TTTCCAAGGT GACGGCCACG TCGACAACAT CATCGTCGAT AGCTGCTACT 
TTAACCCATA ATAAAATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610927.3
CDS	295..3495
Translation	MKESGSVSKNQPQTSEARGSIITYSPSKFLTSVGTGRTTYSTHTHTTTATSAATTATKTNNLPMGGSSAAYYVVKAGSLGSAASMSGCIKVSSPPKGPPMAKSPALVVTSPCTPTESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHNDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_137083.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant B (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137083.4

ORF Insert Sequence:

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACAATGATGC CCAGGACTAT GGAGATGCCC AAACCACTGC AGGTGGTTCA 
GCGAGCCTGC CAAAGGTGAT AACTCCCGGT GTTTTGGGAA AACAACTGCC GCCCGTCAAT 
CTCGCAGCTG GCACCAAGAT TAAGATGATC CCATCCAGCC AGTTCGTTCA AATCAAATCG 
CTACCGTCAC AGAGCAAACT CATTAACTAC ACGCTAAACA AACCGAGTGT CGGGACAAGC 
AGCACCGCCA ACATTGGCAG CGTGGTGAAG ACCCTACCAT GCTCATCCGT TAGTGGCCAG 
CAGATCATCA CCTTAAAAAC GCCCTCGGGA CAAACGCAGC AGTTTGCAAC AGCAGCCACA 
TCAACAGGTG CCGCTGGTTC ATCGACATCA ACAGGAACGG GACAGAAAAA GTACACAGTT 
TTTAAGAACG CCAACGGCAT GACCATGCTG CATCTCACCA AGAGCGTACC CACTACAGGT 
TCTAGCAGCG GCTCTGCGAA TATGGATTTG TCCAACATTA TCGATATGCC CATTGTTTTT 
GCCGATAACG AAGGCAATAT TCCCGAGCAC CAACAGGCGG ATAAGGATAT TAAACCAGCC 
CCCATTCGAA GTGCGAATAA GAGCCCGTTG ATCATCAGTC AGAAAATCAT TAAGGAGGCA 
AATAAGCCCT CGGGCATGGT TACCAGTGGC AGCATTACTG CCGTCAGCAA GCCGGGGAAT 
ATTGTGCTTA ACAAAGGCCT TCAGCAATTG CTAAATTCAT CGCCCGGTGG AACACTTGCC 
ACTCAGAATA AAGTTGTGTA CCTCAACCGG AGTACAATTA AGCCAATGGG AAATGTGGCG 
CCTGGTACTC ATCGTGTCCC TATAAGGATT GTGAGCACCC CAAACACCGG AGGTGCAGTG 
ACGACCACAG CTTCCAGTAG TCCCATTATG GTTGATCCAG CCACAGGATC CAAGGTTGCC 
AAGACCGTCA ATGTGCAGAC AATCAAGCCT ACTGGTTCCG TTCTGGCTCA AGGCACCATC 
CGTCAAGCGG GCAACGTGGG CACCAAGAGC TACCTCCAGA TGATCAATAC CGCACCTGGT 
GCATCCAAAA CTGTTGCTGG CGACGGCAAA GGTTCTGTAC GAAATATCTA CTTTAAATCG 
GCAACCGGAC TCAAGCAGTT GCCAGTGCAG ATGCTAGGCA ATCGAATACC GGGTGGCGCC 
GTCATTTCGT CAACCGCATC GGCATCTTCT GGATCTCCTG GCTTGCGGCG AGTGGTAAAC 
ATTGGGCCCG TTTCCAAGGT GACGGCCACG TCGACAACAT CATCGTCGAT AGCTGCTACT 
TTAACCCATA ATAAAATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rcd1 ( NM_001299475.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286404.1 Drosophila melanogaster reduction in Cnn dots 1, transcript variant H (Rcd1), mRNA.

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CloneID	OFa21799
Clone ID Related Accession (Same CDS sequence)	NM_001299475.1
Accession Version	NM_001299475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reduction in Cnn dots 1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACAATCGCGT CCACAAGCCG AAGCCACCGC GCCTCATTGA TCCGTTTGCT 
GTGAAGCGAA AGGTTGGAAG ACCCCGAACA CGCCCCCTTC CCAATGTCGG TGGCTCGGCA 
CGCTCTGTTA GCCAAAAAGA TGATGCCCAG GACTATGGAG ATGCCCAAAC CACTGCAGGT 
GGTTCAGCGA GCCTGCCAAA GGTGATAACT CCCGGTGTTT TGGGAAAACA ACTGCCGCCC 
GTCAATCTCG CAGCTGGCAC CAAGATTAAG ATGATCCCAT CCAGCCAGTT CGTTCAAATC 
AAATCGCTAC CGTCACAGAG CAAACTCATT AACTACACGC TAAACAAACC GAGTGTCGGG 
ACAAGCAGCA CCGCCAACAT TGGCAGCGTG GTGAAGACCC TACCATGCTC ATCCGTTAGT 
GGCCAGCAGA TCATCACCTT AAAAACGCCC TCGGGACAAA CGCAGCAGTT TGCAACAGCA 
GCCACATCAA CAGGTGCCGC TGGTTCATCG ACATCAACAG GAACGGGACA GAAAAAGTAC 
ACAGTTTTTA AGAACGCCAA CGGCATGACC ATGCTGCATC TCACCAAGAG CGTACCCACT 
ACAGGTTCTA GCAGCGGCTC TGCGAATATG GATTTGTCCA ACATTATCGA TATGCCCATT 
GTTTTTGCCG ATAACGAAGG CAATATTCCC GAGCACCAAC AGGCGGATAA GGATATTAAA 
CCAGCCCCCA TTCGAAGTGC GAATAAGAGC CCGTTGATCA TCAGTCAGAA AATCATTAAG 
GAGGCAAATA AGCCCTCGGG CATGGTTACC AGTGGCAGCA TTACTGCCGT CAGCAAGCCG 
GGGAATATTG TGCTTAACAA AGGCCTTCAG CAATTGCTAA ATTCATCGCC CGGTGGAACA 
CTTGCCACTC AGAATAAAGT TGTGTACCTC AACCGGAGTA CAATTAAGCC AATGGGAAAT 
GTGGCGCCTG GTACTCATCG TGTCCCTATA AGGATTGTGA GCACCCCAAA CACCGGAGGT 
GCAGTGACGA CCACAGCTTC CAGTAGTCCC ATTATGGTTG ATCCAGCCAC AGGATCCAAG 
GTTGCCAAGA CCGTCAATGT GCAGACAATC AAGCCTACTG GTTCCGTTCT GGCTCAAGGC 
ACCATCCGTC AAGCGGGCAA CGTGGGCACC AAGAGCTACC TCCAGATGAT CAATACCGCA 
CCTGGTGCAT CCAAAACTGT TGCTGGCGAC GGCAAAGGTT CTGTACGAAA TATCTACTTT 
AAATCGGCAA CCGGACTCAA GCAGTTGCCA GTGCAGATGC TAGGCAATCG AATACCGGGT 
GGCGCCGTCA TTTCGTCAAC CGCATCGGCA TCTTCTGGAT CTCCTGGCTT GCGGCGAGTG 
GTAAACATTG GGCCCGTTTC CAAGGTGACG GCCACGTCGA CAACATCATC GTCGATAGCT 
GCTACTTTAA CCCATAATAA AATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286404.1
CDS	295..3621
Translation	MKESGSVSKNQPQTSEARGSIITYSPSKFLTSVGTGRTTYSTHTHTTTATSAATTATKTNNLPMGGSSAAYYVVKAGSLGSAASMSGCIKVSSPPKGPPMAKSPALVVTSPCTPTESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHNRVHKPKPPRLIDPFAVKRKVGRPRTRPLPNVGGSARSVSQKDDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM

Target ORF information:

RefSeq Version	NM_001299475.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reduction in Cnn dots 1, transcript variant H (Rcd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299475.1

ORF Insert Sequence:

ATGAAGGAAT CGGGAAGTGT CAGCAAAAAT CAGCCGCAGA CCTCAGAGGC CAGAGGCTCG 
ATTATAACCT ACAGTCCCAG CAAATTTCTG ACTAGCGTTG GAACAGGCAG AACGACATAC 
AGCACACATA CACACACAAC AACAGCAACG TCGGCGGCGA CAACCGCAAC AAAAACAAAC 
AATTTACCAA TGGGCGGATC CAGTGCCGCT TATTATGTGG TAAAGGCTGG CTCCCTGGGC 
AGCGCTGCCT CCATGTCGGG TTGCATCAAA GTCAGTTCTC CGCCAAAGGG GCCGCCGATG 
GCTAAGTCGC CCGCCCTGGT CGTTACATCG CCCTGCACGC CAACAGAGAG TTCCAGTACC 
ATTTCGGCCC CTAGTGAATC CTCCTTTAGC GAGAAAATGG AGCACAGCTA TATGCGAGAT 
ACGCCAGTGC GCAGCGATGT TCACAACGGC CTAACGCCCG CTAGGAACAT TCTGGTCCGC 
CATCCGCCAC AGTGTCCCAG TTGCCACACA TATCCGCCGC AGCACGAGGA GCTGTCAGAG 
ATTCAGCAGG CCCATGTGCC GCCCTATGAT GAGGTAGTCG CCAAGGAGGC AATGAATGAA 
TGCGCTCGTA TTGCAAAATA CGTAAAGAAC AACAATTCGG ATGAGCAAGA TTGGGTGGCG 
CGCGTTAATA AATTTAATTG GACGCCCATG CAGGAGATGG TTTTCGAGAA GGTCTGCTCA 
ATTCTGGACC AGGATCAGTT GGCACGCTTG GCCAACGACA AACGTCAGCA CGAGCCTATC 
CATCGTCGCA TAAGCTCGGA CAAATCCGCG TCCAGGTTTC GCAAACTTCT AGCCAGCGTG 
GCTTGGGAGT CACGCATTAC GCAGTGGATC CATGCTCTGC TCATGGAGCA CTTGTCGCCT 
TCGTATATGG CCTCTTATCT GGAAATTCTT CAGACTCTGA AGACCAAGTT GCCCACTTTA 
GTGGACAAGA TGCTGTTTGG TAGGCCCTTA AATAATAGTC AGGAACTGCT GGCACCGGTG 
ATGAAAAAAC GATGGGAACC AAATATCATG CCCAAGTCCC GTCAGCTAAC GCATAACGCC 
ATCATGGTGG TGCTGCCCAA CATGCCGACA ACTGGAACAG TTTCGGACCG CATGCAGAAG 
TGGTACCAGG CATTATCTAC TATCACTCAG GTGGTTCAGA TTTCGCTGCC GAATACAAAC 
AACAGAATTG GGAATCAGAA TCTGGACCAT GTGGCCGAAA CCATTGTGTC TATCACACGC 
GTCAAAATCA ACGAATTGCG CACAGAAAAC CCCAGTCGTG GCATCATACT CGTTGGATTT 
AATGCTGGAG CTGCCTTGGC TCTACAGGTT GCTCTATCGG AGAGCGTTGC CTGTGTGGTT 
TGCATGGGAT TTGCTTACAA CACAATACGT GGGCCACGCG GAACCCCCGA TGACCGCATG 
TTGGACATAA AAGCGCCGAT ACTCTTTGTC ATCGGACAAA ATTCCGCACG CACAAGTCAG 
GAGGAAATGG AAGGGTTACG CGAACGAATG CAGTCCGAAT CTTCGTTGGT CGTAGTGGGT 
AGTGCAGATG ATGCACTGCG TGTGCCTAAG AGCAAGCGGC GAATAGAAGG CGTTACACAG 
TCGATGGTTG ATTACATGGT TGTCGAGGAA ATCTTTGAGT TTGTGAACAG AACGCTAAGC 
AATCCACCTG GACCGCGTAT GCCCACATGT TTAATGCATC AGCAGGGCTA CCAGCGACTG 
CCAAAGCAGT CACCTCACAT CCTGGCCGAT GGAAACGCAA ACAAGGCTGT TCAGCAGATG 
CGTAAGCGAA AGATGGACGG TGGTTTAGAC GACTCAATGG GTCAGCCTTC CAAAACAAAG 
TTTGTTCCTC ACAATCGCGT CCACAAGCCG AAGCCACCGC GCCTCATTGA TCCGTTTGCT 
GTGAAGCGAA AGGTTGGAAG ACCCCGAACA CGCCCCCTTC CCAATGTCGG TGGCTCGGCA 
CGCTCTGTTA GCCAAAAAGA TGATGCCCAG GACTATGGAG ATGCCCAAAC CACTGCAGGT 
GGTTCAGCGA GCCTGCCAAA GGTGATAACT CCCGGTGTTT TGGGAAAACA ACTGCCGCCC 
GTCAATCTCG CAGCTGGCAC CAAGATTAAG ATGATCCCAT CCAGCCAGTT CGTTCAAATC 
AAATCGCTAC CGTCACAGAG CAAACTCATT AACTACACGC TAAACAAACC GAGTGTCGGG 
ACAAGCAGCA CCGCCAACAT TGGCAGCGTG GTGAAGACCC TACCATGCTC ATCCGTTAGT 
GGCCAGCAGA TCATCACCTT AAAAACGCCC TCGGGACAAA CGCAGCAGTT TGCAACAGCA 
GCCACATCAA CAGGTGCCGC TGGTTCATCG ACATCAACAG GAACGGGACA GAAAAAGTAC 
ACAGTTTTTA AGAACGCCAA CGGCATGACC ATGCTGCATC TCACCAAGAG CGTACCCACT 
ACAGGTTCTA GCAGCGGCTC TGCGAATATG GATTTGTCCA ACATTATCGA TATGCCCATT 
GTTTTTGCCG ATAACGAAGG CAATATTCCC GAGCACCAAC AGGCGGATAA GGATATTAAA 
CCAGCCCCCA TTCGAAGTGC GAATAAGAGC CCGTTGATCA TCAGTCAGAA AATCATTAAG 
GAGGCAAATA AGCCCTCGGG CATGGTTACC AGTGGCAGCA TTACTGCCGT CAGCAAGCCG 
GGGAATATTG TGCTTAACAA AGGCCTTCAG CAATTGCTAA ATTCATCGCC CGGTGGAACA 
CTTGCCACTC AGAATAAAGT TGTGTACCTC AACCGGAGTA CAATTAAGCC AATGGGAAAT 
GTGGCGCCTG GTACTCATCG TGTCCCTATA AGGATTGTGA GCACCCCAAA CACCGGAGGT 
GCAGTGACGA CCACAGCTTC CAGTAGTCCC ATTATGGTTG ATCCAGCCAC AGGATCCAAG 
GTTGCCAAGA CCGTCAATGT GCAGACAATC AAGCCTACTG GTTCCGTTCT GGCTCAAGGC 
ACCATCCGTC AAGCGGGCAA CGTGGGCACC AAGAGCTACC TCCAGATGAT CAATACCGCA 
CCTGGTGCAT CCAAAACTGT TGCTGGCGAC GGCAAAGGTT CTGTACGAAA TATCTACTTT 
AAATCGGCAA CCGGACTCAA GCAGTTGCCA GTGCAGATGC TAGGCAATCG AATACCGGGT 
GGCGCCGTCA TTTCGTCAAC CGCATCGGCA TCTTCTGGAT CTCCTGGCTT GCGGCGAGTG 
GTAAACATTG GGCCCGTTTC CAAGGTGACG GCCACGTCGA CAACATCATC GTCGATAGCT 
GCTACTTTAA CCCATAATAA AATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rcd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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