tum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tum
Entrez Gene ID	36538
Full Name	tumbleweed
Synonyms	13345,AcGAP,CG13345,DRacGAP,Dmel\CG13345,RacGAP,RacGAP50C,RacGAP50c,RacGap,RacGap50C,RhoGAP-50C14,TUM,Tumbleweed,acGAP,eon,i249,racGAP50,racGAP50C,racGAP50c
General protein information	Preferred Names tumbleweed Names CG13345 gene product from transcript CG13345-RA CG13345-PA RacGAP50C enhancer of naked tum-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49C-50D

mRNA and Protein(s)

mRNA	Protein	Name
NM_137068.4	NP_610912.2	tumbleweed

Pathways from BioSystems

Homologies

	RACGAP1	XP_001110799.1
Rattus norvegicus (Norway rat)	Racgap1	NP_001101582.1
Homo sapiens (human)	RACGAP1	NP_001119575.1
Pan troglodytes (chimpanzee)	RACGAP1	XP_001156681.1
Bos taurus (cattle)	RACGAP1	XP_005206410.1
Danio rerio (zebrafish)	racgap1	NP_955925.1
Xenopus tropicalis (tropical clawed frog)	racgap1	NP_001001236.1
Mus musculus (house mouse)	Racgap1	NP_001240737.1
Rattus norvegicus (Norway rat)	LOC100909901	XP_003752175.1
Gallus gallus (chicken)	RACGAP1	XP_004949789.1
Drosophila melanogaster (fruit fly)	tum	NP_610912.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008912	XP_319660.4
Canis lupus familiaris (dog)	RACGAP1	XP_543675.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RhoGEF2, Pbl, RhoGEF3, and Tum exhibit discrete localization patterns and are required for cell wound repair.
Title: Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.

Data show that GTPase-activating protein (Tum/RacGAP) is required for the motor activity of kinesin-6 motor (Pav/kinesin-6).
Title: Tum/RacGAP functions as a switch activating the Pav/kinesin-6 motor.

Study demonstrates the emerging role of centralspindlin (complex of two proteins Tum and Pav) in regulating cell migration and cell adhesion in addition to its previously known function during cytokinesis.
Title: Centralspindlin is required for thorax development during Drosophila metamorphosis.

a new domain of Polo kinase, termed the intermediate domain, interacts directly with RacGAP50C and that Polo kinase is essential for localization of the KLP-RacGAP centralspindlin complex to the cell equator and spindle midzone.
Title: Polo kinase interacts with RacGAP50C and is required to localize the cytokinesis initiation complex.

Results identify two modulators of the Wnt pathway, Tum and Pav, that have shared functions in cell division, which hints at a possible link between cytokinesis and Wnt activity during tumorigenesis.
Title: Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.

The following tum gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tum cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12500	NM_137068.4 Latest version!	Drosophila melanogaster tumbleweed (tum), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tum ( NM_137068.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610912.2 Drosophila melanogaster tumbleweed (tum), mRNA.

Price & Availability↑

CloneID	OFa12500
Clone ID Related Accession (Same CDS sequence)	NM_137068.4
Accession Version	NM_137068.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tumbleweed
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137068.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTCT CCGCATTGGC GTCCTTCGAT GATCTGCGAC GCTGCATGCA AGTGCTTACC 
GATGGAACTC CTGAGGAGGA GTTCCTGCGG TTCTTGCGCA TGTTCGAGCA GTACCACGAA 
AAGTGCGCGG GCTACGCGGC CGAGACGGCC AGGATACAAA ACGAGCTGGA CAAGTCGTTG 
ACCAAAATGG GCGACCTGGA GGGCAAACTC TTTCACGCAC GCCGCATCAT CGACATGGAG 
ATCAAGGCGC GCCGCCAGGC GGAACACGAA CGAGATGCCA TGGAGAGCAA GATCATGGCT 
GTGGCAGATC TGCTGCGACA CGAGCGCAAT CTGAACAACG AGACCCGCGA CAAGCTGGCC 
TTTCTGCACA CACTGCCCTC GTCGCGAAAG CGCAAGTCCC TGAATGCCGT GCGGGAGGAC 
AAGTCCTATG GCGACATCAA CTCCACCGGA TCGTTGCTGT CGGATTTGTC TATTACACAC 
TCCGAGGACG ACTTCCTCGA TGTGCGCACA TCAAAATCTT GGCGAGAGCA TCGTCCTTCG 
CTGCCCAAGA ACCAGATTCC TAGCGTTGGC AACAAGAGAT CGCGATTGAG CACTGGACTG 
AATGGCAGTA TGTCCGGGAC TACACCAACC ACTGGCAAAT CGCGGCGCTC TAGCGTCGGA 
ATCGGAGTGG AGCAACACAC GGTGGACGTG GGTCAAGGCG CTGAGCGCTT TTGTGCCACC 
ACCAAGGTCA CCATACCCCA GGATGGACAG GGCGTGATTC GTGCAGAGTC GACCATTGAG 
TCGCTGCCTG TAATTGCCGG CAACGAGAGG ATCGGCGACG GACTATCCTC GACGCCACGG 
CGATCGGTGC TTAAAGAGGC CACCGCACCG CCGCTGACGC CGGTAAATGC TATGGCACCT 
CACGTTGTCG CTGAATCCGG AACGCCACTG CAGCACCGTC CACTGATGAG GAACCACACC 
TTCAGTCAGA AGACATTCTT GCGTGGCGAC AATTGCGTTC AGTGTCAGAA ACGCATACGG 
TTTGGAGCCG TTGGGCTGAG ATGTCGGGAT TGCCCGGTGC GCTGTCACAT CGATTGTCGG 
TATCTGCTCA CCGTTAGCTG TGTGCCGCAA ACGGGAACAC CGACGACGAA GACCATGACT 
GGCTATGTAA CGGACTTTGC ACCATCTATT GCACCCATGA TCCCGGCACT GATTGTGCAC 
TGCGTAAATG AGATTGAAGC CCGCGGCCTG ACCGAGGTTG GCCTCTATCG ATTGTCCTCG 
TCGGAGCGGG AGTACAAGGC CCTCAAGGAG CAGTTCCTGC GAGGCAAGGC CACTCCACAT 
TTGGGCAACA CGGATATCTA TGTGCTGTGC TGTTGTGTCA AGGATTTCCT GAGGTCCCTA 
ACAGAGCCTC TCATTCCCAC CAGCCAATGG AAGGATTTCG CAAACGCAGT CCAAAATCCT 
GACACCAAAA CTGCCCAGGA TATGTTGGTT AAGTCGGTGA AGCAGCTACC ACAGGCCAAT 
AGGGATACGC TGGCCTTCCT CATACTGCAC TTCCAACGAA TTGCCCAGTG CCCAGTCGTG 
CTAATGCCGA TCGATAACAT CTCGCTCATC TTTGGCCCCA CCATTGTGGG CTACTCCACA 
CCAGATCCCG ACCAGCATGC GATCTACACG GAAGTTTTCA CCCAGAAGCA GGTGATGAAG 
GCACTTCTTG AGCTGCCCGT TTCGTTCTGG GAGCAATATA TCGTTATAGA TCCGACAAGA 
ACACCGGCCA CAGTTATCAA GCGGGTGCCA AGCAACAAAA ATGACTTACT CTCGCTATAT 
GCAACTCCCT TCAAAGGAGG CACCATTAAG AAGCGGAAGT TTTACGGCAC ACCGCCGGCA 
TCTGCGCACA AGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610912.2
CDS	205..2082
Translation	MALSALASFDDLRRCMQVLTDGTPEEEFLRFLRMFEQYHEKCAGYAAETARIQNELDKSLTKMGDLEGKLFHARRIIDMEIKARRQAEHERDAMESKIMAVADLLRHERNLNNETRDKLAFLHTLPSSRKRKSLNAVREDKSYGDINSTGSLLSDLSITHSEDDFLDVRTSKSWREHRPSLPKNQIPSVGNKRSRLSTGLNGSMSGTTPTTGKSRRSSVGIGVEQHTVDVGQGAERFCATTKVTIPQDGQGVIRAESTIESLPVIAGNERIGDGLSSTPRRSVLKEATAPPLTPVNAMAPHVVAESGTPLQHRPLMRNHTFSQKTFLRGDNCVQCQKRIRFGAVGLRCRDCPVRCHIDCRYLLTVSCVPQTGTPTTKTMTGYVTDFAPSIAPMIPALIVHCVNEIEARGLTEVGLYRLSSSEREYKALKEQFLRGKATPHLGNTDIYVLCCCVKDFLRSLTEPLIPTSQWKDFANAVQNPDTKTAQDMLVKSVKQLPQANRDTLAFLILHFQRIAQCPVVLMPIDNISLIFGPTIVGYSTPDPDQHAIYTEVFTQKQVMKALLELPVSFWEQYIVIDPTRTPATVIKRVPSNKNDLLSLYATPFKGGTIKKRKFYGTPPASAHKK

Target ORF information:

RefSeq Version	NM_137068.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tumbleweed (tum), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137068.4

ORF Insert Sequence:

ATGGCGCTCT CCGCATTGGC GTCCTTCGAT GATCTGCGAC GCTGCATGCA AGTGCTTACC 
GATGGAACTC CTGAGGAGGA GTTCCTGCGG TTCTTGCGCA TGTTCGAGCA GTACCACGAA 
AAGTGCGCGG GCTACGCGGC CGAGACGGCC AGGATACAAA ACGAGCTGGA CAAGTCGTTG 
ACCAAAATGG GCGACCTGGA GGGCAAACTC TTTCACGCAC GCCGCATCAT CGACATGGAG 
ATCAAGGCGC GCCGCCAGGC GGAACACGAA CGAGATGCCA TGGAGAGCAA GATCATGGCT 
GTGGCAGATC TGCTGCGACA CGAGCGCAAT CTGAACAACG AGACCCGCGA CAAGCTGGCC 
TTTCTGCACA CACTGCCCTC GTCGCGAAAG CGCAAGTCCC TGAATGCCGT GCGGGAGGAC 
AAGTCCTATG GCGACATCAA CTCCACCGGA TCGTTGCTGT CGGATTTGTC TATTACACAC 
TCCGAGGACG ACTTCCTCGA TGTGCGCACA TCAAAATCTT GGCGAGAGCA TCGTCCTTCG 
CTGCCCAAGA ACCAGATTCC TAGCGTTGGC AACAAGAGAT CGCGATTGAG CACTGGACTG 
AATGGCAGTA TGTCCGGGAC TACACCAACC ACTGGCAAAT CGCGGCGCTC TAGCGTCGGA 
ATCGGAGTGG AGCAACACAC GGTGGACGTG GGTCAAGGCG CTGAGCGCTT TTGTGCCACC 
ACCAAGGTCA CCATACCCCA GGATGGACAG GGCGTGATTC GTGCAGAGTC GACCATTGAG 
TCGCTGCCTG TAATTGCCGG CAACGAGAGG ATCGGCGACG GACTATCCTC GACGCCACGG 
CGATCGGTGC TTAAAGAGGC CACCGCACCG CCGCTGACGC CGGTAAATGC TATGGCACCT 
CACGTTGTCG CTGAATCCGG AACGCCACTG CAGCACCGTC CACTGATGAG GAACCACACC 
TTCAGTCAGA AGACATTCTT GCGTGGCGAC AATTGCGTTC AGTGTCAGAA ACGCATACGG 
TTTGGAGCCG TTGGGCTGAG ATGTCGGGAT TGCCCGGTGC GCTGTCACAT CGATTGTCGG 
TATCTGCTCA CCGTTAGCTG TGTGCCGCAA ACGGGAACAC CGACGACGAA GACCATGACT 
GGCTATGTAA CGGACTTTGC ACCATCTATT GCACCCATGA TCCCGGCACT GATTGTGCAC 
TGCGTAAATG AGATTGAAGC CCGCGGCCTG ACCGAGGTTG GCCTCTATCG ATTGTCCTCG 
TCGGAGCGGG AGTACAAGGC CCTCAAGGAG CAGTTCCTGC GAGGCAAGGC CACTCCACAT 
TTGGGCAACA CGGATATCTA TGTGCTGTGC TGTTGTGTCA AGGATTTCCT GAGGTCCCTA 
ACAGAGCCTC TCATTCCCAC CAGCCAATGG AAGGATTTCG CAAACGCAGT CCAAAATCCT 
GACACCAAAA CTGCCCAGGA TATGTTGGTT AAGTCGGTGA AGCAGCTACC ACAGGCCAAT 
AGGGATACGC TGGCCTTCCT CATACTGCAC TTCCAACGAA TTGCCCAGTG CCCAGTCGTG 
CTAATGCCGA TCGATAACAT CTCGCTCATC TTTGGCCCCA CCATTGTGGG CTACTCCACA 
CCAGATCCCG ACCAGCATGC GATCTACACG GAAGTTTTCA CCCAGAAGCA GGTGATGAAG 
GCACTTCTTG AGCTGCCCGT TTCGTTCTGG GAGCAATATA TCGTTATAGA TCCGACAAGA 
ACACCGGCCA CAGTTATCAA GCGGGTGCCA AGCAACAAAA ATGACTTACT CTCGCTATAT 
GCAACTCCCT TCAAAGGAGG CACCATTAAG AAGCGGAAGT TTTACGGCAC ACCGCCGGCA 
TCTGCGCACA AGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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