FLASH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	FLASH
Entrez Gene ID	36455
Full Name	FLICE-associated huge protein
Synonyms	CG4616,Dmel\CG4616,dFLASH
General protein information	Preferred Names FLICE-associated huge protein Names CG4616 gene product from transcript CG4616-RB CG4616-PA CG4616-PB FLASH ortholog FLASH-PA FLASH-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F8-49F9

mRNA and Protein(s)

mRNA	Protein	Name
NM_137001.4	NP_610845.1	FLICE-associated huge protein, isoform A
NM_206106.3	NP_995828.1	FLICE-associated huge protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila U7 snRNP is associated with FLASH and a complex of polyadenylation factors that resembles the mammalian histone pre-mRNA cleavage complex.
Title: 3'-End processing of histone pre-mRNAs in Drosophila: U7 snRNP is associated with FLASH and polyadenylation factors.

Interaction between FLASH and Lsm11 is essential for histone pre-mRNA processing in vivo in Drosophila
Title: Interaction between FLASH and Lsm11 is essential for histone pre-mRNA processing in vivo in Drosophila.

Drosophila FLASH localizes to histone locus bodies, and its depletion from fly cells inhibits U7-dependent processing, resulting in polyadenylation of histone mRNAs.
Title: FLASH, a proapoptotic protein involved in activation of caspase-8, is essential for 3' end processing of histone pre-mRNAs.

The following FLASH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLASH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12971	NM_206106.3 Latest version!	Drosophila melanogaster FLICE-associated huge protein (FLASH), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12971	NM_137001.4 Latest version!	Drosophila melanogaster FLICE-associated huge protein (FLASH), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FLASH ( NM_137001.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610845.1 Drosophila melanogaster FLICE-associated huge protein (FLASH), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12971
Clone ID Related Accession (Same CDS sequence)	NM_137001.4 , NM_206106.3
Accession Version	NM_137001.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	FLICE-associated huge protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137001.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACGC CTGCATATGC CACGAAATCT GGCGGAGAAC TGGTGCTGCC CGAAGCTCTG 
GAGGACTTGA TGGATTTGGA AGACCAGGAC GCGGGCGGGA TGAAGTCCAA CGTTAAGGAT 
AAATCCATGG ATTTGGATAT CTATCACGAT TTGTACGATA TAGAGCCTGC AGGTTCAAAG 
AAGCCTGACC AAATGGACAG ATCCCTTGAA CTGGACATCT ACGATGATTT GGACGACTTT 
CAGAAGGCCG AAGATCGTAA TACCAAGGAG CTACAGGCAT GGGAAGCCAA ATACGAAAAG 
GCACTGGCCG AAATTGAAGC GCTAAAGGTT GAGAATAAGG CACTTGGCAA GAAGATAAAA 
ACCATGGAGG TTAATCTACA GAATCTACTG GACACAGCTA AGGCGGAGGT AAAGAGAAAA 
GAGACACTGA TTGCCGAGCT GCGAAACGAA AAGGACGATG TATGCTTCCG ACGAAAACGA 
GCACGGGCGT TTGACGTACC AGGTGCAAGG GAACCCGAAA GTAAGCGTCC GAAAGAATTT 
CAATCTACAC CAATCGATGT CAGGAAATAT CCGGAAACGA ATAGAAATCC GTTCAAAACG 
GCTGCAAAGG ACGCCGCTAA GGATGATGCT AGGAAGATGA TGACCAAAAA AGATGACACA 
ACGAAAGTCG TATCAAAAGA TGTTGAAAGA GGCGTAAAAT CCAAGGAGGA TAATAAGATG 
GCGACCAATA AAGATGATGA CAAGAAGTCA TCCTCAAAGG ATGATGCTTG GAAATCTAGG 
ACAAGAGAAG ACGCCAAGAA ATCGGTCCAA AGAAGCAAGT CTCCCAGGCC GGAACACTCA 
AAGACCACCG ATCGGAAATC TACTGCACAG GCTCGTCGTC CAGATCCACA TCGCAGCCGG 
AGTCCAAAAC ATAATTCCCG GCGAGATCAT CACAGGAATC AAGAAAGCAG TTCCCGGCAC 
GCCAAACGGC GAAGCCAATC CGCATCGCCC AGCAAGATAC AATTGCCAAG AGAAAAAGCA 
AATGCCATCA TCACACTTAC TGACGATGAA ACAAATGACC AACGCAGCAA GAGCCCAGTC 
CCCGTGACCG TCAAACTCGT AACTGAATCT CAACCGGCTG AAAAAGTCGT TTCCAACCGA 
AGGGATGCTG CTTTAGAAAC CAATGCAAAT TCCTCAAAGG AATCAAAAGT AGAGACGAAG 
GATATTACTC AACCGACAAA TGGTTTAATT GCTGAACCTT GTATTCCAAA ACATGAGATT 
ATTCCGGGGT TAAGCTTAAT TAACCCAGAA TCGGCGGATT TTTCTAAAAA TGAGATCAAG 
TTAGATCCTG TAATCTTACA GGATCGTACA GAAGACTCTG GCCACCAACT TTTAAATAAG 
GAGCCTATTC CCAAAGTTGT AGTACTTGAA AAATGTAAAA ACATTTTAGC TGAAGATTGC 
CCAGCTGATG AGCAACCCCT GGACAAATTC AAGGATGGAA AAGAATCTGA AGAGACACAG 
GCAAAGTTAG ACAAAAAAGA GCCTTTTGCA GTTGAAACTG CTGTTGAAAC TCCTAGCAAA 
GAAGAAAGCA ACGACAACAT AGTATTATCA AAGAATACGA CGTCTCAAGC TGATGTGTGT 
TCTCAGCATG ATGAGAATGA TGAAGCAGAG ATCCGAGAAC CGATGGATCA AGTTAACTCC 
GTGGTCGAGT CCGACTTGGG AGTAAGGATT ATTGAGGATA TTCGGTTGCC CAAGATGGCG 
AATATCGACC ATTTTGCTGT AAAGGTCGAC AACCATGGTG AAGCGCCAGC AGCTGTCACA 
GATTCCGCTT TAAATCAAGA GAACCTAGCC ACACAGGACC AAAAGATTTG CGCAGAGATG 
AAAGATCCAA CTATTTTTAC CACCTCGGAC GAAACTCCAA TAAAGTCTGC TACTATATTA 
TACGCCAAGC CCGATTCAAC AAAACTGGAT CACAGCGATG AACATGACGA GGTTATCCTG 
GAAACTGCCA TTGACATGCT GAAGAAGGAG CAGGATGCAG CGAGCACCGC AGAATCCACA 
TATCCGAAAC TGCGGATCGC AGAAGATGCC ATTGAGATGG CACTAGAGCA ATTGCACCAG 
CAGTCGCCGG ACGAAACTGT GGTGACCTCA ACGTCGAATA GAGCGCCGCA GACGCCAAAA 
AGCCTCATCA CAATACTCAC CCAGACTCCA AAACAGGCTG ATCCACTAAG ATCCCAAACG 
AAATCGAAGA CTGTGTCGCC GATGGCGACG CCTAAGAAGA CGGCCACGGA GAAGACCCCA 
GTGAAGAAGA GAAAGGTCAA CATGGATAGT TCGCCGCAGG TGCCAGCCCA TGATGTTACC 
ATCGACGAAA CTTTGGGTGC CTGCAGTCTG CAGGACGAGA GCTCAACTGT GACCAAACGC 
TGCTCCCTGG GCCACACAGA CTACCAGTAC GAACAGATAA AGGACGAGGT CATTTTAAGA 
GTGAAACGAC GGGGTCGCCG ACGAAAGCCA ACACCTATGG AAATACCTAC GACTGCTGCG 
GACAAACCAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610845.1
CDS	273..2807
Translation	METPAYATKSGGELVLPEALEDLMDLEDQDAGGMKSNVKDKSMDLDIYHDLYDIEPAGSKKPDQMDRSLELDIYDDLDDFQKAEDRNTKELQAWEAKYEKALAEIEALKVENKALGKKIKTMEVNLQNLLDTAKAEVKRKETLIAELRNEKDDVCFRRKRARAFDVPGAREPESKRPKEFQSTPIDVRKYPETNRNPFKTAAKDAAKDDARKMMTKKDDTTKVVSKDVERGVKSKEDNKMATNKDDDKKSSSKDDAWKSRTREDAKKSVQRSKSPRPEHSKTTDRKSTAQARRPDPHRSRSPKHNSRRDHHRNQESSSRHAKRRSQSASPSKIQLPREKANAIITLTDDETNDQRSKSPVPVTVKLVTESQPAEKVVSNRRDAALETNANSSKESKVETKDITQPTNGLIAEPCIPKHEIIPGLSLINPESADFSKNEIKLDPVILQDRTEDSGHQLLNKEPIPKVVVLEKCKNILAEDCPADEQPLDKFKDGKESEETQAKLDKKEPFAVETAVETPSKEESNDNIVLSKNTTSQADVCSQHDENDEAEIREPMDQVNSVVESDLGVRIIEDIRLPKMANIDHFAVKVDNHGEAPAAVTDSALNQENLATQDQKICAEMKDPTIFTTSDETPIKSATILYAKPDSTKLDHSDEHDEVILETAIDMLKKEQDAASTAESTYPKLRIAEDAIEMALEQLHQQSPDETVVTSTSNRAPQTPKSLITILTQTPKQADPLRSQTKSKTVSPMATPKKTATEKTPVKKRKVNMDSSPQVPAHDVTIDETLGACSLQDESSTVTKRCSLGHTDYQYEQIKDEVILRVKRRGRRRKPTPMEIPTTAADKPI

Target ORF information:

RefSeq Version	NM_137001.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster FLICE-associated huge protein (FLASH), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137001.4

ORF Insert Sequence:

ATGGAAACGC CTGCATATGC CACGAAATCT GGCGGAGAAC TGGTGCTGCC CGAAGCTCTG 
GAGGACTTGA TGGATTTGGA AGACCAGGAC GCGGGCGGGA TGAAGTCCAA CGTTAAGGAT 
AAATCCATGG ATTTGGATAT CTATCACGAT TTGTACGATA TAGAGCCTGC AGGTTCAAAG 
AAGCCTGACC AAATGGACAG ATCCCTTGAA CTGGACATCT ACGATGATTT GGACGACTTT 
CAGAAGGCCG AAGATCGTAA TACCAAGGAG CTACAGGCAT GGGAAGCCAA ATACGAAAAG 
GCACTGGCCG AAATTGAAGC GCTAAAGGTT GAGAATAAGG CACTTGGCAA GAAGATAAAA 
ACCATGGAGG TTAATCTACA GAATCTACTG GACACAGCTA AGGCGGAGGT AAAGAGAAAA 
GAGACACTGA TTGCCGAGCT GCGAAACGAA AAGGACGATG TATGCTTCCG ACGAAAACGA 
GCACGGGCGT TTGACGTACC AGGTGCAAGG GAACCCGAAA GTAAGCGTCC GAAAGAATTT 
CAATCTACAC CAATCGATGT CAGGAAATAT CCGGAAACGA ATAGAAATCC GTTCAAAACG 
GCTGCAAAGG ACGCCGCTAA GGATGATGCT AGGAAGATGA TGACCAAAAA AGATGACACA 
ACGAAAGTCG TATCAAAAGA TGTTGAAAGA GGCGTAAAAT CCAAGGAGGA TAATAAGATG 
GCGACCAATA AAGATGATGA CAAGAAGTCA TCCTCAAAGG ATGATGCTTG GAAATCTAGG 
ACAAGAGAAG ACGCCAAGAA ATCGGTCCAA AGAAGCAAGT CTCCCAGGCC GGAACACTCA 
AAGACCACCG ATCGGAAATC TACTGCACAG GCTCGTCGTC CAGATCCACA TCGCAGCCGG 
AGTCCAAAAC ATAATTCCCG GCGAGATCAT CACAGGAATC AAGAAAGCAG TTCCCGGCAC 
GCCAAACGGC GAAGCCAATC CGCATCGCCC AGCAAGATAC AATTGCCAAG AGAAAAAGCA 
AATGCCATCA TCACACTTAC TGACGATGAA ACAAATGACC AACGCAGCAA GAGCCCAGTC 
CCCGTGACCG TCAAACTCGT AACTGAATCT CAACCGGCTG AAAAAGTCGT TTCCAACCGA 
AGGGATGCTG CTTTAGAAAC CAATGCAAAT TCCTCAAAGG AATCAAAAGT AGAGACGAAG 
GATATTACTC AACCGACAAA TGGTTTAATT GCTGAACCTT GTATTCCAAA ACATGAGATT 
ATTCCGGGGT TAAGCTTAAT TAACCCAGAA TCGGCGGATT TTTCTAAAAA TGAGATCAAG 
TTAGATCCTG TAATCTTACA GGATCGTACA GAAGACTCTG GCCACCAACT TTTAAATAAG 
GAGCCTATTC CCAAAGTTGT AGTACTTGAA AAATGTAAAA ACATTTTAGC TGAAGATTGC 
CCAGCTGATG AGCAACCCCT GGACAAATTC AAGGATGGAA AAGAATCTGA AGAGACACAG 
GCAAAGTTAG ACAAAAAAGA GCCTTTTGCA GTTGAAACTG CTGTTGAAAC TCCTAGCAAA 
GAAGAAAGCA ACGACAACAT AGTATTATCA AAGAATACGA CGTCTCAAGC TGATGTGTGT 
TCTCAGCATG ATGAGAATGA TGAAGCAGAG ATCCGAGAAC CGATGGATCA AGTTAACTCC 
GTGGTCGAGT CCGACTTGGG AGTAAGGATT ATTGAGGATA TTCGGTTGCC CAAGATGGCG 
AATATCGACC ATTTTGCTGT AAAGGTCGAC AACCATGGTG AAGCGCCAGC AGCTGTCACA 
GATTCCGCTT TAAATCAAGA GAACCTAGCC ACACAGGACC AAAAGATTTG CGCAGAGATG 
AAAGATCCAA CTATTTTTAC CACCTCGGAC GAAACTCCAA TAAAGTCTGC TACTATATTA 
TACGCCAAGC CCGATTCAAC AAAACTGGAT CACAGCGATG AACATGACGA GGTTATCCTG 
GAAACTGCCA TTGACATGCT GAAGAAGGAG CAGGATGCAG CGAGCACCGC AGAATCCACA 
TATCCGAAAC TGCGGATCGC AGAAGATGCC ATTGAGATGG CACTAGAGCA ATTGCACCAG 
CAGTCGCCGG ACGAAACTGT GGTGACCTCA ACGTCGAATA GAGCGCCGCA GACGCCAAAA 
AGCCTCATCA CAATACTCAC CCAGACTCCA AAACAGGCTG ATCCACTAAG ATCCCAAACG 
AAATCGAAGA CTGTGTCGCC GATGGCGACG CCTAAGAAGA CGGCCACGGA GAAGACCCCA 
GTGAAGAAGA GAAAGGTCAA CATGGATAGT TCGCCGCAGG TGCCAGCCCA TGATGTTACC 
ATCGACGAAA CTTTGGGTGC CTGCAGTCTG CAGGACGAGA GCTCAACTGT GACCAAACGC 
TGCTCCCTGG GCCACACAGA CTACCAGTAC GAACAGATAA AGGACGAGGT CATTTTAAGA 
GTGAAACGAC GGGGTCGCCG ACGAAAGCCA ACACCTATGG AAATACCTAC GACTGCTGCG 
GACAAACCAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLASH ( NM_206106.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995828.1 Drosophila melanogaster FLICE-associated huge protein (FLASH), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12971
Clone ID Related Accession (Same CDS sequence)	NM_137001.4 , NM_206106.3
Accession Version	NM_206106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	FLICE-associated huge protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_206106.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACGC CTGCATATGC CACGAAATCT GGCGGAGAAC TGGTGCTGCC CGAAGCTCTG 
GAGGACTTGA TGGATTTGGA AGACCAGGAC GCGGGCGGGA TGAAGTCCAA CGTTAAGGAT 
AAATCCATGG ATTTGGATAT CTATCACGAT TTGTACGATA TAGAGCCTGC AGGTTCAAAG 
AAGCCTGACC AAATGGACAG ATCCCTTGAA CTGGACATCT ACGATGATTT GGACGACTTT 
CAGAAGGCCG AAGATCGTAA TACCAAGGAG CTACAGGCAT GGGAAGCCAA ATACGAAAAG 
GCACTGGCCG AAATTGAAGC GCTAAAGGTT GAGAATAAGG CACTTGGCAA GAAGATAAAA 
ACCATGGAGG TTAATCTACA GAATCTACTG GACACAGCTA AGGCGGAGGT AAAGAGAAAA 
GAGACACTGA TTGCCGAGCT GCGAAACGAA AAGGACGATG TATGCTTCCG ACGAAAACGA 
GCACGGGCGT TTGACGTACC AGGTGCAAGG GAACCCGAAA GTAAGCGTCC GAAAGAATTT 
CAATCTACAC CAATCGATGT CAGGAAATAT CCGGAAACGA ATAGAAATCC GTTCAAAACG 
GCTGCAAAGG ACGCCGCTAA GGATGATGCT AGGAAGATGA TGACCAAAAA AGATGACACA 
ACGAAAGTCG TATCAAAAGA TGTTGAAAGA GGCGTAAAAT CCAAGGAGGA TAATAAGATG 
GCGACCAATA AAGATGATGA CAAGAAGTCA TCCTCAAAGG ATGATGCTTG GAAATCTAGG 
ACAAGAGAAG ACGCCAAGAA ATCGGTCCAA AGAAGCAAGT CTCCCAGGCC GGAACACTCA 
AAGACCACCG ATCGGAAATC TACTGCACAG GCTCGTCGTC CAGATCCACA TCGCAGCCGG 
AGTCCAAAAC ATAATTCCCG GCGAGATCAT CACAGGAATC AAGAAAGCAG TTCCCGGCAC 
GCCAAACGGC GAAGCCAATC CGCATCGCCC AGCAAGATAC AATTGCCAAG AGAAAAAGCA 
AATGCCATCA TCACACTTAC TGACGATGAA ACAAATGACC AACGCAGCAA GAGCCCAGTC 
CCCGTGACCG TCAAACTCGT AACTGAATCT CAACCGGCTG AAAAAGTCGT TTCCAACCGA 
AGGGATGCTG CTTTAGAAAC CAATGCAAAT TCCTCAAAGG AATCAAAAGT AGAGACGAAG 
GATATTACTC AACCGACAAA TGGTTTAATT GCTGAACCTT GTATTCCAAA ACATGAGATT 
ATTCCGGGGT TAAGCTTAAT TAACCCAGAA TCGGCGGATT TTTCTAAAAA TGAGATCAAG 
TTAGATCCTG TAATCTTACA GGATCGTACA GAAGACTCTG GCCACCAACT TTTAAATAAG 
GAGCCTATTC CCAAAGTTGT AGTACTTGAA AAATGTAAAA ACATTTTAGC TGAAGATTGC 
CCAGCTGATG AGCAACCCCT GGACAAATTC AAGGATGGAA AAGAATCTGA AGAGACACAG 
GCAAAGTTAG ACAAAAAAGA GCCTTTTGCA GTTGAAACTG CTGTTGAAAC TCCTAGCAAA 
GAAGAAAGCA ACGACAACAT AGTATTATCA AAGAATACGA CGTCTCAAGC TGATGTGTGT 
TCTCAGCATG ATGAGAATGA TGAAGCAGAG ATCCGAGAAC CGATGGATCA AGTTAACTCC 
GTGGTCGAGT CCGACTTGGG AGTAAGGATT ATTGAGGATA TTCGGTTGCC CAAGATGGCG 
AATATCGACC ATTTTGCTGT AAAGGTCGAC AACCATGGTG AAGCGCCAGC AGCTGTCACA 
GATTCCGCTT TAAATCAAGA GAACCTAGCC ACACAGGACC AAAAGATTTG CGCAGAGATG 
AAAGATCCAA CTATTTTTAC CACCTCGGAC GAAACTCCAA TAAAGTCTGC TACTATATTA 
TACGCCAAGC CCGATTCAAC AAAACTGGAT CACAGCGATG AACATGACGA GGTTATCCTG 
GAAACTGCCA TTGACATGCT GAAGAAGGAG CAGGATGCAG CGAGCACCGC AGAATCCACA 
TATCCGAAAC TGCGGATCGC AGAAGATGCC ATTGAGATGG CACTAGAGCA ATTGCACCAG 
CAGTCGCCGG ACGAAACTGT GGTGACCTCA ACGTCGAATA GAGCGCCGCA GACGCCAAAA 
AGCCTCATCA CAATACTCAC CCAGACTCCA AAACAGGCTG ATCCACTAAG ATCCCAAACG 
AAATCGAAGA CTGTGTCGCC GATGGCGACG CCTAAGAAGA CGGCCACGGA GAAGACCCCA 
GTGAAGAAGA GAAAGGTCAA CATGGATAGT TCGCCGCAGG TGCCAGCCCA TGATGTTACC 
ATCGACGAAA CTTTGGGTGC CTGCAGTCTG CAGGACGAGA GCTCAACTGT GACCAAACGC 
TGCTCCCTGG GCCACACAGA CTACCAGTAC GAACAGATAA AGGACGAGGT CATTTTAAGA 
GTGAAACGAC GGGGTCGCCG ACGAAAGCCA ACACCTATGG AAATACCTAC GACTGCTGCG 
GACAAACCAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995828.1
CDS	278..2812
Translation	METPAYATKSGGELVLPEALEDLMDLEDQDAGGMKSNVKDKSMDLDIYHDLYDIEPAGSKKPDQMDRSLELDIYDDLDDFQKAEDRNTKELQAWEAKYEKALAEIEALKVENKALGKKIKTMEVNLQNLLDTAKAEVKRKETLIAELRNEKDDVCFRRKRARAFDVPGAREPESKRPKEFQSTPIDVRKYPETNRNPFKTAAKDAAKDDARKMMTKKDDTTKVVSKDVERGVKSKEDNKMATNKDDDKKSSSKDDAWKSRTREDAKKSVQRSKSPRPEHSKTTDRKSTAQARRPDPHRSRSPKHNSRRDHHRNQESSSRHAKRRSQSASPSKIQLPREKANAIITLTDDETNDQRSKSPVPVTVKLVTESQPAEKVVSNRRDAALETNANSSKESKVETKDITQPTNGLIAEPCIPKHEIIPGLSLINPESADFSKNEIKLDPVILQDRTEDSGHQLLNKEPIPKVVVLEKCKNILAEDCPADEQPLDKFKDGKESEETQAKLDKKEPFAVETAVETPSKEESNDNIVLSKNTTSQADVCSQHDENDEAEIREPMDQVNSVVESDLGVRIIEDIRLPKMANIDHFAVKVDNHGEAPAAVTDSALNQENLATQDQKICAEMKDPTIFTTSDETPIKSATILYAKPDSTKLDHSDEHDEVILETAIDMLKKEQDAASTAESTYPKLRIAEDAIEMALEQLHQQSPDETVVTSTSNRAPQTPKSLITILTQTPKQADPLRSQTKSKTVSPMATPKKTATEKTPVKKRKVNMDSSPQVPAHDVTIDETLGACSLQDESSTVTKRCSLGHTDYQYEQIKDEVILRVKRRGRRRKPTPMEIPTTAADKPI

Target ORF information:

RefSeq Version	NM_206106.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster FLICE-associated huge protein (FLASH), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206106.3

ORF Insert Sequence:

ATGGAAACGC CTGCATATGC CACGAAATCT GGCGGAGAAC TGGTGCTGCC CGAAGCTCTG 
GAGGACTTGA TGGATTTGGA AGACCAGGAC GCGGGCGGGA TGAAGTCCAA CGTTAAGGAT 
AAATCCATGG ATTTGGATAT CTATCACGAT TTGTACGATA TAGAGCCTGC AGGTTCAAAG 
AAGCCTGACC AAATGGACAG ATCCCTTGAA CTGGACATCT ACGATGATTT GGACGACTTT 
CAGAAGGCCG AAGATCGTAA TACCAAGGAG CTACAGGCAT GGGAAGCCAA ATACGAAAAG 
GCACTGGCCG AAATTGAAGC GCTAAAGGTT GAGAATAAGG CACTTGGCAA GAAGATAAAA 
ACCATGGAGG TTAATCTACA GAATCTACTG GACACAGCTA AGGCGGAGGT AAAGAGAAAA 
GAGACACTGA TTGCCGAGCT GCGAAACGAA AAGGACGATG TATGCTTCCG ACGAAAACGA 
GCACGGGCGT TTGACGTACC AGGTGCAAGG GAACCCGAAA GTAAGCGTCC GAAAGAATTT 
CAATCTACAC CAATCGATGT CAGGAAATAT CCGGAAACGA ATAGAAATCC GTTCAAAACG 
GCTGCAAAGG ACGCCGCTAA GGATGATGCT AGGAAGATGA TGACCAAAAA AGATGACACA 
ACGAAAGTCG TATCAAAAGA TGTTGAAAGA GGCGTAAAAT CCAAGGAGGA TAATAAGATG 
GCGACCAATA AAGATGATGA CAAGAAGTCA TCCTCAAAGG ATGATGCTTG GAAATCTAGG 
ACAAGAGAAG ACGCCAAGAA ATCGGTCCAA AGAAGCAAGT CTCCCAGGCC GGAACACTCA 
AAGACCACCG ATCGGAAATC TACTGCACAG GCTCGTCGTC CAGATCCACA TCGCAGCCGG 
AGTCCAAAAC ATAATTCCCG GCGAGATCAT CACAGGAATC AAGAAAGCAG TTCCCGGCAC 
GCCAAACGGC GAAGCCAATC CGCATCGCCC AGCAAGATAC AATTGCCAAG AGAAAAAGCA 
AATGCCATCA TCACACTTAC TGACGATGAA ACAAATGACC AACGCAGCAA GAGCCCAGTC 
CCCGTGACCG TCAAACTCGT AACTGAATCT CAACCGGCTG AAAAAGTCGT TTCCAACCGA 
AGGGATGCTG CTTTAGAAAC CAATGCAAAT TCCTCAAAGG AATCAAAAGT AGAGACGAAG 
GATATTACTC AACCGACAAA TGGTTTAATT GCTGAACCTT GTATTCCAAA ACATGAGATT 
ATTCCGGGGT TAAGCTTAAT TAACCCAGAA TCGGCGGATT TTTCTAAAAA TGAGATCAAG 
TTAGATCCTG TAATCTTACA GGATCGTACA GAAGACTCTG GCCACCAACT TTTAAATAAG 
GAGCCTATTC CCAAAGTTGT AGTACTTGAA AAATGTAAAA ACATTTTAGC TGAAGATTGC 
CCAGCTGATG AGCAACCCCT GGACAAATTC AAGGATGGAA AAGAATCTGA AGAGACACAG 
GCAAAGTTAG ACAAAAAAGA GCCTTTTGCA GTTGAAACTG CTGTTGAAAC TCCTAGCAAA 
GAAGAAAGCA ACGACAACAT AGTATTATCA AAGAATACGA CGTCTCAAGC TGATGTGTGT 
TCTCAGCATG ATGAGAATGA TGAAGCAGAG ATCCGAGAAC CGATGGATCA AGTTAACTCC 
GTGGTCGAGT CCGACTTGGG AGTAAGGATT ATTGAGGATA TTCGGTTGCC CAAGATGGCG 
AATATCGACC ATTTTGCTGT AAAGGTCGAC AACCATGGTG AAGCGCCAGC AGCTGTCACA 
GATTCCGCTT TAAATCAAGA GAACCTAGCC ACACAGGACC AAAAGATTTG CGCAGAGATG 
AAAGATCCAA CTATTTTTAC CACCTCGGAC GAAACTCCAA TAAAGTCTGC TACTATATTA 
TACGCCAAGC CCGATTCAAC AAAACTGGAT CACAGCGATG AACATGACGA GGTTATCCTG 
GAAACTGCCA TTGACATGCT GAAGAAGGAG CAGGATGCAG CGAGCACCGC AGAATCCACA 
TATCCGAAAC TGCGGATCGC AGAAGATGCC ATTGAGATGG CACTAGAGCA ATTGCACCAG 
CAGTCGCCGG ACGAAACTGT GGTGACCTCA ACGTCGAATA GAGCGCCGCA GACGCCAAAA 
AGCCTCATCA CAATACTCAC CCAGACTCCA AAACAGGCTG ATCCACTAAG ATCCCAAACG 
AAATCGAAGA CTGTGTCGCC GATGGCGACG CCTAAGAAGA CGGCCACGGA GAAGACCCCA 
GTGAAGAAGA GAAAGGTCAA CATGGATAGT TCGCCGCAGG TGCCAGCCCA TGATGTTACC 
ATCGACGAAA CTTTGGGTGC CTGCAGTCTG CAGGACGAGA GCTCAACTGT GACCAAACGC 
TGCTCCCTGG GCCACACAGA CTACCAGTAC GAACAGATAA AGGACGAGGT CATTTTAAGA 
GTGAAACGAC GGGGTCGCCG ACGAAAGCCA ACACCTATGG AAATACCTAC GACTGCTGCG 
GACAAACCAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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FLASH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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