Drl-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Drl-2
Entrez Gene ID	36436
Full Name	Derailed 2
Synonyms	CG12463,CG3915,DNT-like,DRL-2,Dmel\CG3915,dnl,drl-2
General protein information	Preferred Names Derailed 2 Names CG3915 gene product from transcript CG3915-RC CG3915-PB CG3915-PC Drl-2-PB Drl-2-PC derailed 2 derailed-2 doughnut-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F1-49F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_136991.4	NP_610835.2	derailed 2, isoform B
NM_001299442.1	NP_001286371.1	derailed 2, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ryk	NP_038677.3
Bos taurus (cattle)	RYK	XP_002685146.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006405	XP_316438.5
Pan troglodytes (chimpanzee)	RYK	XP_003310084.1
Homo sapiens (human)	RYK	NP_001005861.1
Gallus gallus (chicken)	RYK	XP_422674.4
Caenorhabditis elegans (roundworm)	lin-18	NP_508684.4
Xenopus tropicalis (tropical clawed frog)	ryk	NP_001017272.1
Canis lupus familiaris (dog)	RYK	XP_005634509.1
Rattus norvegicus (Norway rat)	Ryk	NP_536327.1
Danio rerio (zebrafish)	ryk	NP_001077295.1
Drosophila melanogaster (fruit fly)	Drl-2	NP_610835.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Forced expression of Drl-2 in the glia of drl mutants rescues the glomerular phenotype caused by the loss of antagonistic Drl function.
Title: Differentially expressed Drl and Drl-2 play opposing roles in Wnt5 signaling during Drosophila olfactory system development.

The following Drl-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Drl-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12956	NM_136991.4 Latest version!	Drosophila melanogaster derailed 2, transcript variant B (Drl-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12956	NM_001299442.1 Latest version!	Drosophila melanogaster derailed 2, transcript variant C (Drl-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Drl-2 ( NM_136991.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610835.2 Drosophila melanogaster derailed 2, transcript variant B (Drl-2), mRNA.

Price & Availability↑

CloneID	OFa12956
Clone ID Related Accession (Same CDS sequence)	NM_136991.4 , NM_001299442.1
Accession Version	NM_136991.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	derailed 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136991.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACTCC TTCCTCTGAA GTCGCCCCTC CCGATCCTGC TCCTATGCCT AACCATTTCC 
ACTTCGGTCA GCGGCTATCT GAACATTTTC ATCAGCCACC ACGAGGTGAT GAAACTGATG 
GGACTCGAGG CGGACCTGTT CTATGTGCAC GAGGGAGCCA TCAACACATA CGCAATGCAC 
TTCACCGTCC CAGTGCCCGC GGATGTCCAT GAGCTGGAGT TTTCCTGGCA GAGTCTGATT 
GCCTACCCAC TGCCATACGC CATTAGTATT GAGTATAACA ACGACCAGGA GGCACTGGGC 
ACGCCCACGT TGAGCATTCC GCACAAGGGT CTGGTGCCAC AGGAGATCGA GTCCTTCCTG 
GTTTACCTGC CCTGCACCGG AAATGCGAGC CTACAGATGC CCGTCAATGT CAACATGGTA 
GTACGGGCTC CTCCTCGATT CAACGACACC CGGCTCCACT TTAAGCGCAA TAAGATCTGC 
GCCAAAGGCA TCTCACCTGA GCCCAATCAA TCGCCGGCGC CAGCCCACGC ACCCTCCCAA 
GGACCCGCCC TCCTCAGTGC CGCCGCCTGT GCTCTGGGTT TGGTTTTGGC CGTGGGACTA 
GTGGCCAGCA TGATGTATGT GAGGGCGCGC AAACAGCTTC GCCAGGACTC GCTACACACC 
AGTTTCACCA CCGCAGCTTA TGGCAGCCAC CAAAACGTGT TCATCCGACT GGATCCCCTG 
GGAAGACCGC CGAGTGCCAC GGGATCCTAT GCGACCATTG CCAGCCTCAA CAAATACCCT 
GCGGACAGCA AAAAGTCGTG CAGCATATTC GACCGTTTCC GGAGCTCACC CACCCCCACG 
CCGTATGCCA CCGCCCTGCT GCCCATGAAC CTTGAACAGA CCGCAGAGAC GATTTACTCG 
AAGCCAGAGT CGATCTGTCC CTCGAGGATT TCCTACTACG CCTCCTCGCA ACTGACCCAG 
CCTTGCAGCC TATCGACGCC GAAGAGCATC CGAAGCAACG GCAATGGCAA CTGCACCAGC 
GGAAGTGGCA GCCTAAGCCT TTTTGGAGGC ATCCCCACCG GCAGCACCAT CACCATGGCC 
AGTCACGGGG AGAAGGGTAA CCAACGGCTG CGACGGATAA CCAGCGTCCA GCCCGGTGCC 
CTCAGCTACG AGGAGCTGGT CAAGGAGGGC ACCTTCGGCC GGATTTACGC CGGCAAGCTG 
GGCGAGTCAT GCGAGGCGCT CGTAAAGACC GTCATCGATG GCGCATCGCT CACCCAGGTG 
GCCTGCCTGC TGCAGGACGC CTCCCTGCTG ATCGGCGTCA GTCATCAGCA CATCCTGGCA 
CCGCTGTTGG CCAACACGGA GCTGCCGGGT CCACCGGAGA TTGCCTATCC CCATCCGTCC 
AAGGGCAATC TCAAGATGTA CTTGCAAAAG TCGCGGGAAT CGAGCACAGC ATTGAGTACG 
CGCCAACTGG TTGAGTTTGG GCTGCACATC ACCAAGGGAT TGGCCTATCT GCACTCACTG 
GGAATCGTTC ACAAGGACAT TGCTACACGC AATTGCTACC TTGACGAAGA GTCCTATGTG 
AAGATCTGCG ATAGCGCCCT CTCTCGCGAT CTCTTCCCAG ACGACTACGA CTGTCTGGGC 
GACAATGAGA ATCGCCCGCT GAAGTGGCTG TCGCTGGAAT CCCTCCAGAA GCGGGTCTAT 
GCCACCCAGG GCGACGTGTG GGCCTTGGGC GTGACCTACT GGGAGCTGGT CACTCTGGCG 
CAGATGCCGC ACGAGGAGGT GGATATATTC GAGCTTACCA ATTACTTGGC TGCCGGCTTC 
CGGCTGGAGC AGCCAGTCAA CTGCCCAGAT GAATTCTTTA CGGTTATGAA CTGCTGCTGG 
CACTGCGAGG CCAAACAGCG ACCTACGCCC TCACAGTTGC TATCGTATCT GCAGGACTTC 
CATGCGGATC TGGGCATGTA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610835.2
CDS	1210..3156
Translation	MELLPLKSPLPILLLCLTISTSVSGYLNIFISHHEVMKLMGLEADLFYVHEGAINTYAMHFTVPVPADVHELEFSWQSLIAYPLPYAISIEYNNDQEALGTPTLSIPHKGLVPQEIESFLVYLPCTGNASLQMPVNVNMVVRAPPRFNDTRLHFKRNKICAKGISPEPNQSPAPAHAPSQGPALLSAAACALGLVLAVGLVASMMYVRARKQLRQDSLHTSFTTAAYGSHQNVFIRLDPLGRPPSATGSYATIASLNKYPADSKKSCSIFDRFRSSPTPTPYATALLPMNLEQTAETIYSKPESICPSRISYYASSQLTQPCSLSTPKSIRSNGNGNCTSGSGSLSLFGGIPTGSTITMASHGEKGNQRLRRITSVQPGALSYEELVKEGTFGRIYAGKLGESCEALVKTVIDGASLTQVACLLQDASLLIGVSHQHILAPLLANTELPGPPEIAYPHPSKGNLKMYLQKSRESSTALSTRQLVEFGLHITKGLAYLHSLGIVHKDIATRNCYLDEESYVKICDSALSRDLFPDDYDCLGDNENRPLKWLSLESLQKRVYATQGDVWALGVTYWELVTLAQMPHEEVDIFELTNYLAAGFRLEQPVNCPDEFFTVMNCCWHCEAKQRPTPSQLLSYLQDFHADLGMYI

Target ORF information:

RefSeq Version	NM_136991.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster derailed 2, transcript variant B (Drl-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136991.4

ORF Insert Sequence:

ATGGAACTCC TTCCTCTGAA GTCGCCCCTC CCGATCCTGC TCCTATGCCT AACCATTTCC 
ACTTCGGTCA GCGGCTATCT GAACATTTTC ATCAGCCACC ACGAGGTGAT GAAACTGATG 
GGACTCGAGG CGGACCTGTT CTATGTGCAC GAGGGAGCCA TCAACACATA CGCAATGCAC 
TTCACCGTCC CAGTGCCCGC GGATGTCCAT GAGCTGGAGT TTTCCTGGCA GAGTCTGATT 
GCCTACCCAC TGCCATACGC CATTAGTATT GAGTATAACA ACGACCAGGA GGCACTGGGC 
ACGCCCACGT TGAGCATTCC GCACAAGGGT CTGGTGCCAC AGGAGATCGA GTCCTTCCTG 
GTTTACCTGC CCTGCACCGG AAATGCGAGC CTACAGATGC CCGTCAATGT CAACATGGTA 
GTACGGGCTC CTCCTCGATT CAACGACACC CGGCTCCACT TTAAGCGCAA TAAGATCTGC 
GCCAAAGGCA TCTCACCTGA GCCCAATCAA TCGCCGGCGC CAGCCCACGC ACCCTCCCAA 
GGACCCGCCC TCCTCAGTGC CGCCGCCTGT GCTCTGGGTT TGGTTTTGGC CGTGGGACTA 
GTGGCCAGCA TGATGTATGT GAGGGCGCGC AAACAGCTTC GCCAGGACTC GCTACACACC 
AGTTTCACCA CCGCAGCTTA TGGCAGCCAC CAAAACGTGT TCATCCGACT GGATCCCCTG 
GGAAGACCGC CGAGTGCCAC GGGATCCTAT GCGACCATTG CCAGCCTCAA CAAATACCCT 
GCGGACAGCA AAAAGTCGTG CAGCATATTC GACCGTTTCC GGAGCTCACC CACCCCCACG 
CCGTATGCCA CCGCCCTGCT GCCCATGAAC CTTGAACAGA CCGCAGAGAC GATTTACTCG 
AAGCCAGAGT CGATCTGTCC CTCGAGGATT TCCTACTACG CCTCCTCGCA ACTGACCCAG 
CCTTGCAGCC TATCGACGCC GAAGAGCATC CGAAGCAACG GCAATGGCAA CTGCACCAGC 
GGAAGTGGCA GCCTAAGCCT TTTTGGAGGC ATCCCCACCG GCAGCACCAT CACCATGGCC 
AGTCACGGGG AGAAGGGTAA CCAACGGCTG CGACGGATAA CCAGCGTCCA GCCCGGTGCC 
CTCAGCTACG AGGAGCTGGT CAAGGAGGGC ACCTTCGGCC GGATTTACGC CGGCAAGCTG 
GGCGAGTCAT GCGAGGCGCT CGTAAAGACC GTCATCGATG GCGCATCGCT CACCCAGGTG 
GCCTGCCTGC TGCAGGACGC CTCCCTGCTG ATCGGCGTCA GTCATCAGCA CATCCTGGCA 
CCGCTGTTGG CCAACACGGA GCTGCCGGGT CCACCGGAGA TTGCCTATCC CCATCCGTCC 
AAGGGCAATC TCAAGATGTA CTTGCAAAAG TCGCGGGAAT CGAGCACAGC ATTGAGTACG 
CGCCAACTGG TTGAGTTTGG GCTGCACATC ACCAAGGGAT TGGCCTATCT GCACTCACTG 
GGAATCGTTC ACAAGGACAT TGCTACACGC AATTGCTACC TTGACGAAGA GTCCTATGTG 
AAGATCTGCG ATAGCGCCCT CTCTCGCGAT CTCTTCCCAG ACGACTACGA CTGTCTGGGC 
GACAATGAGA ATCGCCCGCT GAAGTGGCTG TCGCTGGAAT CCCTCCAGAA GCGGGTCTAT 
GCCACCCAGG GCGACGTGTG GGCCTTGGGC GTGACCTACT GGGAGCTGGT CACTCTGGCG 
CAGATGCCGC ACGAGGAGGT GGATATATTC GAGCTTACCA ATTACTTGGC TGCCGGCTTC 
CGGCTGGAGC AGCCAGTCAA CTGCCCAGAT GAATTCTTTA CGGTTATGAA CTGCTGCTGG 
CACTGCGAGG CCAAACAGCG ACCTACGCCC TCACAGTTGC TATCGTATCT GCAGGACTTC 
CATGCGGATC TGGGCATGTA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Drl-2 ( NM_001299442.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286371.1 Drosophila melanogaster derailed 2, transcript variant C (Drl-2), mRNA.

Price & Availability↑

CloneID	OFa12956
Clone ID Related Accession (Same CDS sequence)	NM_136991.4 , NM_001299442.1
Accession Version	NM_001299442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	derailed 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACTCC TTCCTCTGAA GTCGCCCCTC CCGATCCTGC TCCTATGCCT AACCATTTCC 
ACTTCGGTCA GCGGCTATCT GAACATTTTC ATCAGCCACC ACGAGGTGAT GAAACTGATG 
GGACTCGAGG CGGACCTGTT CTATGTGCAC GAGGGAGCCA TCAACACATA CGCAATGCAC 
TTCACCGTCC CAGTGCCCGC GGATGTCCAT GAGCTGGAGT TTTCCTGGCA GAGTCTGATT 
GCCTACCCAC TGCCATACGC CATTAGTATT GAGTATAACA ACGACCAGGA GGCACTGGGC 
ACGCCCACGT TGAGCATTCC GCACAAGGGT CTGGTGCCAC AGGAGATCGA GTCCTTCCTG 
GTTTACCTGC CCTGCACCGG AAATGCGAGC CTACAGATGC CCGTCAATGT CAACATGGTA 
GTACGGGCTC CTCCTCGATT CAACGACACC CGGCTCCACT TTAAGCGCAA TAAGATCTGC 
GCCAAAGGCA TCTCACCTGA GCCCAATCAA TCGCCGGCGC CAGCCCACGC ACCCTCCCAA 
GGACCCGCCC TCCTCAGTGC CGCCGCCTGT GCTCTGGGTT TGGTTTTGGC CGTGGGACTA 
GTGGCCAGCA TGATGTATGT GAGGGCGCGC AAACAGCTTC GCCAGGACTC GCTACACACC 
AGTTTCACCA CCGCAGCTTA TGGCAGCCAC CAAAACGTGT TCATCCGACT GGATCCCCTG 
GGAAGACCGC CGAGTGCCAC GGGATCCTAT GCGACCATTG CCAGCCTCAA CAAATACCCT 
GCGGACAGCA AAAAGTCGTG CAGCATATTC GACCGTTTCC GGAGCTCACC CACCCCCACG 
CCGTATGCCA CCGCCCTGCT GCCCATGAAC CTTGAACAGA CCGCAGAGAC GATTTACTCG 
AAGCCAGAGT CGATCTGTCC CTCGAGGATT TCCTACTACG CCTCCTCGCA ACTGACCCAG 
CCTTGCAGCC TATCGACGCC GAAGAGCATC CGAAGCAACG GCAATGGCAA CTGCACCAGC 
GGAAGTGGCA GCCTAAGCCT TTTTGGAGGC ATCCCCACCG GCAGCACCAT CACCATGGCC 
AGTCACGGGG AGAAGGGTAA CCAACGGCTG CGACGGATAA CCAGCGTCCA GCCCGGTGCC 
CTCAGCTACG AGGAGCTGGT CAAGGAGGGC ACCTTCGGCC GGATTTACGC CGGCAAGCTG 
GGCGAGTCAT GCGAGGCGCT CGTAAAGACC GTCATCGATG GCGCATCGCT CACCCAGGTG 
GCCTGCCTGC TGCAGGACGC CTCCCTGCTG ATCGGCGTCA GTCATCAGCA CATCCTGGCA 
CCGCTGTTGG CCAACACGGA GCTGCCGGGT CCACCGGAGA TTGCCTATCC CCATCCGTCC 
AAGGGCAATC TCAAGATGTA CTTGCAAAAG TCGCGGGAAT CGAGCACAGC ATTGAGTACG 
CGCCAACTGG TTGAGTTTGG GCTGCACATC ACCAAGGGAT TGGCCTATCT GCACTCACTG 
GGAATCGTTC ACAAGGACAT TGCTACACGC AATTGCTACC TTGACGAAGA GTCCTATGTG 
AAGATCTGCG ATAGCGCCCT CTCTCGCGAT CTCTTCCCAG ACGACTACGA CTGTCTGGGC 
GACAATGAGA ATCGCCCGCT GAAGTGGCTG TCGCTGGAAT CCCTCCAGAA GCGGGTCTAT 
GCCACCCAGG GCGACGTGTG GGCCTTGGGC GTGACCTACT GGGAGCTGGT CACTCTGGCG 
CAGATGCCGC ACGAGGAGGT GGATATATTC GAGCTTACCA ATTACTTGGC TGCCGGCTTC 
CGGCTGGAGC AGCCAGTCAA CTGCCCAGAT GAATTCTTTA CGGTTATGAA CTGCTGCTGG 
CACTGCGAGG CCAAACAGCG ACCTACGCCC TCACAGTTGC TATCGTATCT GCAGGACTTC 
CATGCGGATC TGGGCATGTA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286371.1
CDS	1210..3156
Translation	MELLPLKSPLPILLLCLTISTSVSGYLNIFISHHEVMKLMGLEADLFYVHEGAINTYAMHFTVPVPADVHELEFSWQSLIAYPLPYAISIEYNNDQEALGTPTLSIPHKGLVPQEIESFLVYLPCTGNASLQMPVNVNMVVRAPPRFNDTRLHFKRNKICAKGISPEPNQSPAPAHAPSQGPALLSAAACALGLVLAVGLVASMMYVRARKQLRQDSLHTSFTTAAYGSHQNVFIRLDPLGRPPSATGSYATIASLNKYPADSKKSCSIFDRFRSSPTPTPYATALLPMNLEQTAETIYSKPESICPSRISYYASSQLTQPCSLSTPKSIRSNGNGNCTSGSGSLSLFGGIPTGSTITMASHGEKGNQRLRRITSVQPGALSYEELVKEGTFGRIYAGKLGESCEALVKTVIDGASLTQVACLLQDASLLIGVSHQHILAPLLANTELPGPPEIAYPHPSKGNLKMYLQKSRESSTALSTRQLVEFGLHITKGLAYLHSLGIVHKDIATRNCYLDEESYVKICDSALSRDLFPDDYDCLGDNENRPLKWLSLESLQKRVYATQGDVWALGVTYWELVTLAQMPHEEVDIFELTNYLAAGFRLEQPVNCPDEFFTVMNCCWHCEAKQRPTPSQLLSYLQDFHADLGMYI

Target ORF information:

RefSeq Version	NM_001299442.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster derailed 2, transcript variant C (Drl-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299442.1

ORF Insert Sequence:

ATGGAACTCC TTCCTCTGAA GTCGCCCCTC CCGATCCTGC TCCTATGCCT AACCATTTCC 
ACTTCGGTCA GCGGCTATCT GAACATTTTC ATCAGCCACC ACGAGGTGAT GAAACTGATG 
GGACTCGAGG CGGACCTGTT CTATGTGCAC GAGGGAGCCA TCAACACATA CGCAATGCAC 
TTCACCGTCC CAGTGCCCGC GGATGTCCAT GAGCTGGAGT TTTCCTGGCA GAGTCTGATT 
GCCTACCCAC TGCCATACGC CATTAGTATT GAGTATAACA ACGACCAGGA GGCACTGGGC 
ACGCCCACGT TGAGCATTCC GCACAAGGGT CTGGTGCCAC AGGAGATCGA GTCCTTCCTG 
GTTTACCTGC CCTGCACCGG AAATGCGAGC CTACAGATGC CCGTCAATGT CAACATGGTA 
GTACGGGCTC CTCCTCGATT CAACGACACC CGGCTCCACT TTAAGCGCAA TAAGATCTGC 
GCCAAAGGCA TCTCACCTGA GCCCAATCAA TCGCCGGCGC CAGCCCACGC ACCCTCCCAA 
GGACCCGCCC TCCTCAGTGC CGCCGCCTGT GCTCTGGGTT TGGTTTTGGC CGTGGGACTA 
GTGGCCAGCA TGATGTATGT GAGGGCGCGC AAACAGCTTC GCCAGGACTC GCTACACACC 
AGTTTCACCA CCGCAGCTTA TGGCAGCCAC CAAAACGTGT TCATCCGACT GGATCCCCTG 
GGAAGACCGC CGAGTGCCAC GGGATCCTAT GCGACCATTG CCAGCCTCAA CAAATACCCT 
GCGGACAGCA AAAAGTCGTG CAGCATATTC GACCGTTTCC GGAGCTCACC CACCCCCACG 
CCGTATGCCA CCGCCCTGCT GCCCATGAAC CTTGAACAGA CCGCAGAGAC GATTTACTCG 
AAGCCAGAGT CGATCTGTCC CTCGAGGATT TCCTACTACG CCTCCTCGCA ACTGACCCAG 
CCTTGCAGCC TATCGACGCC GAAGAGCATC CGAAGCAACG GCAATGGCAA CTGCACCAGC 
GGAAGTGGCA GCCTAAGCCT TTTTGGAGGC ATCCCCACCG GCAGCACCAT CACCATGGCC 
AGTCACGGGG AGAAGGGTAA CCAACGGCTG CGACGGATAA CCAGCGTCCA GCCCGGTGCC 
CTCAGCTACG AGGAGCTGGT CAAGGAGGGC ACCTTCGGCC GGATTTACGC CGGCAAGCTG 
GGCGAGTCAT GCGAGGCGCT CGTAAAGACC GTCATCGATG GCGCATCGCT CACCCAGGTG 
GCCTGCCTGC TGCAGGACGC CTCCCTGCTG ATCGGCGTCA GTCATCAGCA CATCCTGGCA 
CCGCTGTTGG CCAACACGGA GCTGCCGGGT CCACCGGAGA TTGCCTATCC CCATCCGTCC 
AAGGGCAATC TCAAGATGTA CTTGCAAAAG TCGCGGGAAT CGAGCACAGC ATTGAGTACG 
CGCCAACTGG TTGAGTTTGG GCTGCACATC ACCAAGGGAT TGGCCTATCT GCACTCACTG 
GGAATCGTTC ACAAGGACAT TGCTACACGC AATTGCTACC TTGACGAAGA GTCCTATGTG 
AAGATCTGCG ATAGCGCCCT CTCTCGCGAT CTCTTCCCAG ACGACTACGA CTGTCTGGGC 
GACAATGAGA ATCGCCCGCT GAAGTGGCTG TCGCTGGAAT CCCTCCAGAA GCGGGTCTAT 
GCCACCCAGG GCGACGTGTG GGCCTTGGGC GTGACCTACT GGGAGCTGGT CACTCTGGCG 
CAGATGCCGC ACGAGGAGGT GGATATATTC GAGCTTACCA ATTACTTGGC TGCCGGCTTC 
CGGCTGGAGC AGCCAGTCAA CTGCCCAGAT GAATTCTTTA CGGTTATGAA CTGCTGCTGG 
CACTGCGAGG CCAAACAGCG ACCTACGCCC TCACAGTTGC TATCGTATCT GCAGGACTTC 
CATGCGGATC TGGGCATGTA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Drl-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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会社情報