Lfg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lfg
Entrez Gene ID	36418
Full Name	CG3814 gene product from transcript CG3814-RB
Synonyms	BEST:CK02213,CK02213,Dmel\CG3814
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49D6-49D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_136980.4	NP_610824.1	lifeguard, isoform A
NM_001273998.1	NP_001260927.1	lifeguard, isoform C
NM_165955.3	NP_725236.1	lifeguard, isoform B
NM_001273999.1	NP_001260928.1	lifeguard, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG3814	NP_725236.1
	LOC708234	XP_001098441.1
Mus musculus (house mouse)	Grina	NP_075657.1
Danio rerio (zebrafish)	zgc:136572	NP_001038775.1
Pan troglodytes (chimpanzee)	GRINA	XP_520009.2
Rattus norvegicus (Norway rat)	Grina	NP_695220.4
Canis lupus familiaris (dog)	GRINA	XP_532348.3
Bos taurus (cattle)	GRINA	NP_001032682.1
Homo sapiens (human)	GRINA	NP_001009184.1
Drosophila melanogaster (fruit fly)	Nmda1	NP_523722.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that Knockdown of CG3814/LFG in Ddc-Gal4-expressing neurons in Drosophila diminishes its neuroprotective ability and results in a shortened lifespan and loss of climbing ability, phenotypes that are improved upon overexpression of the pro-survival Buffy.
Title: Knockdown of the putative Lifeguard homologue CG3814 in neurons of Drosophila melanogaster.

The following Lfg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lfg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09828	NM_136980.4 Latest version!	Drosophila melanogaster lifeguard, transcript variant A (Lfg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09828	NM_001273999.1 Latest version!	Drosophila melanogaster lifeguard, transcript variant D (Lfg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09829	NM_001273998.1 Latest version!	Drosophila melanogaster lifeguard, transcript variant C (Lfg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa12961	NM_165955.3 Latest version!	Drosophila melanogaster lifeguard, transcript variant B (Lfg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lfg ( NM_136980.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610824.1 Drosophila melanogaster lifeguard, transcript variant A (Lfg), mRNA.

Price & Availability↑

CloneID	OFa09828
Clone ID Related Accession (Same CDS sequence)	NM_136980.4 , NM_001273999.1
Accession Version	NM_136980.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lifeguard, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136980.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG ACAATCATTT TCAGTACGAT GCGGAAGCGG ACAAGAGCTT CGCCTTCGAT 
GACCAGAGCA TCCGGAAGGG ATTTATTCGA AAGGTCTACT TGATATTGAT GTGCCAATTG 
CTGATCACTT TCGGTTTTGT TAGCGTATTT ACGTTCTCGA AAGCTTCGCA GGAATGGGTG 
CAAAAAAATC CGGCACTGTT TTGGATAGCC TTGGCCGTTC TTATCGTGAC CATGATCTGT 
ATGGCCTGCT GCGAAAGTGT ACGCCGTAAG ACGCCTCTTA ACTTCATCTT TCTTTTCCTG 
TTCACCGTGG CGGAGTCGTT CCTTCTGGGT ATGGTCGCCG GACAGTTTGA GGCTGATGAG 
GTCCTGATGG CAGTGGGAAT TACGGCCGCG GTGGCCCTGG GACTCACCCT GTTTGCCCTG 
CAGACCAAAT ACGATTTTAC GATGTGCGGA GGAGTGTTGG TGGCCTGTCT GGTGGTCTTC 
ATCATTTTTG GTATAATCGC TATCTTCATA CCGGGCAAGG TGATCGGACT GGTCTATGCC 
TCCTTGGGAG CATTGCTCTT CTCCGTTTAC TTGGTGTACG ATACCCAGTT GATGCTGGGT 
GGTAATCATA AGTACTCCAT CAGTCCTGAG GAATACATCT TCGCCGCTCT AAACCTCTAC 
CTGGACATTA TTAACATCTT CATGTACATA CTGACCATTA TCGGATTGTC TCGCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610824.1
CDS	245..964
Translation	MSSDNHFQYDAEADKSFAFDDQSIRKGFIRKVYLILMCQLLITFGFVSVFTFSKASQEWVQKNPALFWIALAVLIVTMICMACCESVRRKTPLNFIFLFLFTVAESFLLGMVAGQFEADEVLMAVGITAAVALGLTLFALQTKYDFTMCGGVLVACLVVFIIFGIIAIFIPGKVIGLVYASLGALLFSVYLVYDTQLMLGGNHKYSISPEEYIFAALNLYLDIINIFMYILTIIGLSRN

Target ORF information:

RefSeq Version	NM_136980.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lifeguard, transcript variant A (Lfg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136980.4

ORF Insert Sequence:

ATGAGCAGCG ACAATCATTT TCAGTACGAT GCGGAAGCGG ACAAGAGCTT CGCCTTCGAT 
GACCAGAGCA TCCGGAAGGG ATTTATTCGA AAGGTCTACT TGATATTGAT GTGCCAATTG 
CTGATCACTT TCGGTTTTGT TAGCGTATTT ACGTTCTCGA AAGCTTCGCA GGAATGGGTG 
CAAAAAAATC CGGCACTGTT TTGGATAGCC TTGGCCGTTC TTATCGTGAC CATGATCTGT 
ATGGCCTGCT GCGAAAGTGT ACGCCGTAAG ACGCCTCTTA ACTTCATCTT TCTTTTCCTG 
TTCACCGTGG CGGAGTCGTT CCTTCTGGGT ATGGTCGCCG GACAGTTTGA GGCTGATGAG 
GTCCTGATGG CAGTGGGAAT TACGGCCGCG GTGGCCCTGG GACTCACCCT GTTTGCCCTG 
CAGACCAAAT ACGATTTTAC GATGTGCGGA GGAGTGTTGG TGGCCTGTCT GGTGGTCTTC 
ATCATTTTTG GTATAATCGC TATCTTCATA CCGGGCAAGG TGATCGGACT GGTCTATGCC 
TCCTTGGGAG CATTGCTCTT CTCCGTTTAC TTGGTGTACG ATACCCAGTT GATGCTGGGT 
GGTAATCATA AGTACTCCAT CAGTCCTGAG GAATACATCT TCGCCGCTCT AAACCTCTAC 
CTGGACATTA TTAACATCTT CATGTACATA CTGACCATTA TCGGATTGTC TCGCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lfg ( NM_001273999.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260928.1 Drosophila melanogaster lifeguard, transcript variant D (Lfg), mRNA.

Price & Availability↑

CloneID	OFa09828
Clone ID Related Accession (Same CDS sequence)	NM_136980.4 , NM_001273999.1
Accession Version	NM_001273999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lifeguard, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCG ACAATCATTT TCAGTACGAT GCGGAAGCGG ACAAGAGCTT CGCCTTCGAT 
GACCAGAGCA TCCGGAAGGG ATTTATTCGA AAGGTCTACT TGATATTGAT GTGCCAATTG 
CTGATCACTT TCGGTTTTGT TAGCGTATTT ACGTTCTCGA AAGCTTCGCA GGAATGGGTG 
CAAAAAAATC CGGCACTGTT TTGGATAGCC TTGGCCGTTC TTATCGTGAC CATGATCTGT 
ATGGCCTGCT GCGAAAGTGT ACGCCGTAAG ACGCCTCTTA ACTTCATCTT TCTTTTCCTG 
TTCACCGTGG CGGAGTCGTT CCTTCTGGGT ATGGTCGCCG GACAGTTTGA GGCTGATGAG 
GTCCTGATGG CAGTGGGAAT TACGGCCGCG GTGGCCCTGG GACTCACCCT GTTTGCCCTG 
CAGACCAAAT ACGATTTTAC GATGTGCGGA GGAGTGTTGG TGGCCTGTCT GGTGGTCTTC 
ATCATTTTTG GTATAATCGC TATCTTCATA CCGGGCAAGG TGATCGGACT GGTCTATGCC 
TCCTTGGGAG CATTGCTCTT CTCCGTTTAC TTGGTGTACG ATACCCAGTT GATGCTGGGT 
GGTAATCATA AGTACTCCAT CAGTCCTGAG GAATACATCT TCGCCGCTCT AAACCTCTAC 
CTGGACATTA TTAACATCTT CATGTACATA CTGACCATTA TCGGATTGTC TCGCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260928.1
CDS	105..824
Translation	MSSDNHFQYDAEADKSFAFDDQSIRKGFIRKVYLILMCQLLITFGFVSVFTFSKASQEWVQKNPALFWIALAVLIVTMICMACCESVRRKTPLNFIFLFLFTVAESFLLGMVAGQFEADEVLMAVGITAAVALGLTLFALQTKYDFTMCGGVLVACLVVFIIFGIIAIFIPGKVIGLVYASLGALLFSVYLVYDTQLMLGGNHKYSISPEEYIFAALNLYLDIINIFMYILTIIGLSRN

Target ORF information:

RefSeq Version	NM_001273999.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lifeguard, transcript variant D (Lfg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273999.1

ORF Insert Sequence:

ATGAGCAGCG ACAATCATTT TCAGTACGAT GCGGAAGCGG ACAAGAGCTT CGCCTTCGAT 
GACCAGAGCA TCCGGAAGGG ATTTATTCGA AAGGTCTACT TGATATTGAT GTGCCAATTG 
CTGATCACTT TCGGTTTTGT TAGCGTATTT ACGTTCTCGA AAGCTTCGCA GGAATGGGTG 
CAAAAAAATC CGGCACTGTT TTGGATAGCC TTGGCCGTTC TTATCGTGAC CATGATCTGT 
ATGGCCTGCT GCGAAAGTGT ACGCCGTAAG ACGCCTCTTA ACTTCATCTT TCTTTTCCTG 
TTCACCGTGG CGGAGTCGTT CCTTCTGGGT ATGGTCGCCG GACAGTTTGA GGCTGATGAG 
GTCCTGATGG CAGTGGGAAT TACGGCCGCG GTGGCCCTGG GACTCACCCT GTTTGCCCTG 
CAGACCAAAT ACGATTTTAC GATGTGCGGA GGAGTGTTGG TGGCCTGTCT GGTGGTCTTC 
ATCATTTTTG GTATAATCGC TATCTTCATA CCGGGCAAGG TGATCGGACT GGTCTATGCC 
TCCTTGGGAG CATTGCTCTT CTCCGTTTAC TTGGTGTACG ATACCCAGTT GATGCTGGGT 
GGTAATCATA AGTACTCCAT CAGTCCTGAG GAATACATCT TCGCCGCTCT AAACCTCTAC 
CTGGACATTA TTAACATCTT CATGTACATA CTGACCATTA TCGGATTGTC TCGCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lfg ( NM_001273998.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260927.1 Drosophila melanogaster lifeguard, transcript variant C (Lfg), mRNA.

Price & Availability↑

CloneID	OFa09829
Clone ID Related Accession (Same CDS sequence)	NM_001273998.1
Accession Version	NM_001273998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lifeguard, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCCAAT TGCTGATCAC TTTCGGTTTT GTTAGCGTAT TTACGTTCTC GAAAGCTTCG 
CAGGAATGGG TGCAAAAAAA TCCGGCACTG TTTTGGATAG CCTTGGCCGT TCTTATCGTG 
ACCATGATCT GTATGGCCTG CTGCGAAAGT GTACGCCGTA AGACGCCTCT TAACTTCATC 
TTTCTTTTCC TGTTCACCGT GGCGGAGTCG TTCCTTCTGG GTATGGTCGC CGGACAGTTT 
GAGGCTGATG AGGTCCTGAT GGCAGTGGGA ATTACGGCCG CGGTGGCCCT GGGACTCACC 
CTGTTTGCCC TGCAGACCAA ATACGATTTT ACGATGTGCG GAGGAGTGTT GGTGGCCTGT 
CTGGTGGTCT TCATCATTTT TGGTATAATC GCTATCTTCA TACCGGGCAA GGTGATCGGA 
CTGGTCTATG CCTCCTTGGG AGCATTGCTC TTCTCCGTTT ACTTGGTGTA CGATACCCAG 
TTGATGCTGG GTGGTAATCA TAAGTACTCC ATCAGTCCTG AGGAATACAT CTTCGCCGCT 
CTAAACCTCT ACCTGGACAT TATTAACATC TTCATGTACA TACTGACCAT TATCGGATTG 
TCTCGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260927.1
CDS	291..902
Translation	MCQLLITFGFVSVFTFSKASQEWVQKNPALFWIALAVLIVTMICMACCESVRRKTPLNFIFLFLFTVAESFLLGMVAGQFEADEVLMAVGITAAVALGLTLFALQTKYDFTMCGGVLVACLVVFIIFGIIAIFIPGKVIGLVYASLGALLFSVYLVYDTQLMLGGNHKYSISPEEYIFAALNLYLDIINIFMYILTIIGLSRN

Target ORF information:

RefSeq Version	NM_001273998.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lifeguard, transcript variant C (Lfg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273998.1

ORF Insert Sequence:

ATGTGCCAAT TGCTGATCAC TTTCGGTTTT GTTAGCGTAT TTACGTTCTC GAAAGCTTCG 
CAGGAATGGG TGCAAAAAAA TCCGGCACTG TTTTGGATAG CCTTGGCCGT TCTTATCGTG 
ACCATGATCT GTATGGCCTG CTGCGAAAGT GTACGCCGTA AGACGCCTCT TAACTTCATC 
TTTCTTTTCC TGTTCACCGT GGCGGAGTCG TTCCTTCTGG GTATGGTCGC CGGACAGTTT 
GAGGCTGATG AGGTCCTGAT GGCAGTGGGA ATTACGGCCG CGGTGGCCCT GGGACTCACC 
CTGTTTGCCC TGCAGACCAA ATACGATTTT ACGATGTGCG GAGGAGTGTT GGTGGCCTGT 
CTGGTGGTCT TCATCATTTT TGGTATAATC GCTATCTTCA TACCGGGCAA GGTGATCGGA 
CTGGTCTATG CCTCCTTGGG AGCATTGCTC TTCTCCGTTT ACTTGGTGTA CGATACCCAG 
TTGATGCTGG GTGGTAATCA TAAGTACTCC ATCAGTCCTG AGGAATACAT CTTCGCCGCT 
CTAAACCTCT ACCTGGACAT TATTAACATC TTCATGTACA TACTGACCAT TATCGGATTG 
TCTCGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lfg ( NM_165955.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725236.1 Drosophila melanogaster lifeguard, transcript variant B (Lfg), mRNA.

Price & Availability↑

CloneID	OFa12961
Clone ID Related Accession (Same CDS sequence)	NM_165955.3
Accession Version	NM_165955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lifeguard, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165955.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCACT ACCAGCAAGG CGATGAAACT GGCGCATACA CAGATGCGGA AGCGGACAAG 
AGCTTCGCCT TCGATGACCA GAGCATCCGG AAGGGATTTA TTCGAAAGGT CTACTTGATA 
TTGATGTGCC AATTGCTGAT CACTTTCGGT TTTGTTAGCG TATTTACGTT CTCGAAAGCT 
TCGCAGGAAT GGGTGCAAAA AAATCCGGCA CTGTTTTGGA TAGCCTTGGC CGTTCTTATC 
GTGACCATGA TCTGTATGGC CTGCTGCGAA AGTGTACGCC GTAAGACGCC TCTTAACTTC 
ATCTTTCTTT TCCTGTTCAC CGTGGCGGAG TCGTTCCTTC TGGGTATGGT CGCCGGACAG 
TTTGAGGCTG ATGAGGTCCT GATGGCAGTG GGAATTACGG CCGCGGTGGC CCTGGGACTC 
ACCCTGTTTG CCCTGCAGAC CAAATACGAT TTTACGATGT GCGGAGGAGT GTTGGTGGCC 
TGTCTGGTGG TCTTCATCAT TTTTGGTATA ATCGCTATCT TCATACCGGG CAAGGTGATC 
GGACTGGTCT ATGCCTCCTT GGGAGCATTG CTCTTCTCCG TTTACTTGGT GTACGATACC 
CAGTTGATGC TGGGTGGTAA TCATAAGTAC TCCATCAGTC CTGAGGAATA CATCTTCGCC 
GCTCTAAACC TCTACCTGGA CATTATTAAC ATCTTCATGT ACATACTGAC CATTATCGGA 
TTGTCTCGCA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725236.1
CDS	177..911
Translation	MYHYQQGDETGAYTDAEADKSFAFDDQSIRKGFIRKVYLILMCQLLITFGFVSVFTFSKASQEWVQKNPALFWIALAVLIVTMICMACCESVRRKTPLNFIFLFLFTVAESFLLGMVAGQFEADEVLMAVGITAAVALGLTLFALQTKYDFTMCGGVLVACLVVFIIFGIIAIFIPGKVIGLVYASLGALLFSVYLVYDTQLMLGGNHKYSISPEEYIFAALNLYLDIINIFMYILTIIGLSRN

Target ORF information:

RefSeq Version	NM_165955.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lifeguard, transcript variant B (Lfg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165955.3

ORF Insert Sequence:

ATGTATCACT ACCAGCAAGG CGATGAAACT GGCGCATACA CAGATGCGGA AGCGGACAAG 
AGCTTCGCCT TCGATGACCA GAGCATCCGG AAGGGATTTA TTCGAAAGGT CTACTTGATA 
TTGATGTGCC AATTGCTGAT CACTTTCGGT TTTGTTAGCG TATTTACGTT CTCGAAAGCT 
TCGCAGGAAT GGGTGCAAAA AAATCCGGCA CTGTTTTGGA TAGCCTTGGC CGTTCTTATC 
GTGACCATGA TCTGTATGGC CTGCTGCGAA AGTGTACGCC GTAAGACGCC TCTTAACTTC 
ATCTTTCTTT TCCTGTTCAC CGTGGCGGAG TCGTTCCTTC TGGGTATGGT CGCCGGACAG 
TTTGAGGCTG ATGAGGTCCT GATGGCAGTG GGAATTACGG CCGCGGTGGC CCTGGGACTC 
ACCCTGTTTG CCCTGCAGAC CAAATACGAT TTTACGATGT GCGGAGGAGT GTTGGTGGCC 
TGTCTGGTGG TCTTCATCAT TTTTGGTATA ATCGCTATCT TCATACCGGG CAAGGTGATC 
GGACTGGTCT ATGCCTCCTT GGGAGCATTG CTCTTCTCCG TTTACTTGGT GTACGATACC 
CAGTTGATGC TGGGTGGTAA TCATAAGTAC TCCATCAGTC CTGAGGAATA CATCTTCGCC 
GCTCTAAACC TCTACCTGGA CATTATTAAC ATCTTCATGT ACATACTGAC CATTATCGGA 
TTGTCTCGCA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lfg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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