Nup188 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup188
Entrez Gene ID	36397
Full Name	CG8771 gene product from transcript CG8771-RB
Synonyms	Dmel\CG8771,Nup188
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49B12-49B12

mRNA and Protein(s)

mRNA	Protein	Name
NM_136966.4	NP_610810.2	nucleoporin 188kDa, isoform B
NM_001259354.2	NP_001246283.1	nucleoporin 188kDa, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG8771	NP_001246283.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005193	XP_314091.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nup188 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup188 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09803	NM_136966.4 Latest version!	Drosophila melanogaster nucleoporin 188kDa, transcript variant B (Nup188), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12176	NM_001259354.2 Latest version!	Drosophila melanogaster nucleoporin 188kDa, transcript variant C (Nup188), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup188 ( NM_136966.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610810.2 Drosophila melanogaster nucleoporin 188kDa, transcript variant B (Nup188), mRNA.

Price & Availability↑

CloneID	OFa09803
Clone ID Related Accession (Same CDS sequence)	NM_136966.4
Accession Version	NM_136966.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 188kDa, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136966.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTGCGG TGGAAAAGAG CGTGATCACC GATTGGAAGC GCCTGTGGCC GATGGTCTCA 
GGAATCCACT ATGAGACGCC GCAGGATACT GTACGTGAGG AGCTGATGAA CGTGGCTTCT 
GAATTGCAGG CTGGAGTGCT CCAATTTAAG CCCAAGAATG CCTCCAGCCT CGAGCTGGGC 
ACGTTGCTCA AAGAGAAAAA GCAGGAAAAG CTGCTGCCCT TCACCGAACG ACTGCAGGAT 
CTGTTGGATC TAGAATCCGC CCAGTGCTGG GAAATCCTTT GCTACTACCT TACGCAGGAG 
TACCGTGGAT CGGCGAGTCT CCTTACCCAA CTAATCTCTA CCGAAACGAA TATGGCCAAA 
CTGCACGAAG ACATACGGCA TTACTACTCC CTGGAACGCA TGGTTGTCCT CAAGATTGTT 
AAGAATCTTA TCGTCTTTCA CCAGGTACCG AATCATCCCT ATCACCGGGA GTATCGCGCC 
GTGGTCGAAA AGATAACCAT CCCCCGCCTT AGAGATTCAT ACCTAGACCA ACTGGAGAGC 
CTTATTTGCG AAGTCCCACC CAGAAAGCTA ATGGCGGGAG AATGCTTCCA CTCTGCGGAA 
CGCTTAGTTG CCTGGTCGGA GCGAAATGCT CGTGAGATCA ACGAGGTGCT GCACATCCTT 
CTGGTGCTAG CGGAACACCT GCCCATGGGG TTGGAGCAGA TAAAGAGAAT ATTTGCCGCC 
TGCAAGCAAC ACTCCTTTGG AAAGATGCAA AGTTATCTGG ATGACAGCCA ACCTTACCAT 
CAGGAGATTA TTCGAAGCCT TTCTTACTCA GAGCTAATGT TAGTTTTAAA GTGCCTCGAC 
TTTGAGAAGC CCGAGAAGCA TTCCGATCTA ATTGAAAAGC TTATAGAGGA TCTGCAGGTT 
GATATTGCCA GCATGTACCA CAGACCAGAG CATGGCCCTT TGCTCCTAGC GTGGATGCTG 
CTTCGCTTGC GAGGCACCAA CGATGCCGAC GATGCCTCCA GCTTGCTCCG CTGTCGGCAG 
CTGGGCAAGC GAGCCGTGGA TCTTAAGTGT TTTGTCCAAC TGCACCTAAT TGCCAGACAT 
TCTATGTACG CCGATGACTC TATGCTGTCA CGGATTGTGC GAAGAACCAT ATACAACCAG 
GTTGGATATC TGTGCGACCT CTTCGACGGC GATGGCAGCT GCGCTCGGTA TGAGGGCATT 
TACGAATTGC TCTGCGAACT TGTCTCATGG CCACACTTAG CTAAAGACTT TTGCAGTCGG 
GAAGATGATG GTCCGTGTTC GCTGTACAAA ACTCTTTTAG AGAACTTCCC TCTAGAATTA 
ACACATCTGT CCAAACTGGC ACTGTCACTG ACAAAAGCGG GACAAGGCAA CTATGTCAAG 
AGCCAACTAG AGGCTTTGCC CATATTGGCT CTTCGTTACG ACGAAAGTCA GCACAAACTC 
AGAGAAGTGG ACACAAATGA ATTCGAGCTC CTGGCCAGCG TGCAACCATT TCAGCAAATT 
GATTTTACCA TACCCGCAGG AACTAGTTGC ACAGCCATCC AACATCCGTC CGGCTGCTTT 
ATGCACTTTC GTTTCCCGGT AAACTACTTT GACGCGCTGC ACCACGAAAT TAACTGCCTG 
CTACGCGAGA CGGGTCACCT GCATGGTGAC TTTGAATCGA GCGAACGCAT ACGCAATGTA 
GAAGCTGGTT TAAGATTTTT GGAAAGTGCC GTTAAGCTCA GTCAATCGAT ATCCGGCATC 
AGCGCCGAAA TGGTGCATCC TACCGAGATG TGCGTTGATC TGCTGCACAC GTTCAAAAGC 
GTTCAATACC CACCGGTTGG ACTTTTGTCT AGCTGTCTCA ATGTTTGCAC TGCGCTGCTG 
CCGTTGGTAG ACGAGGAAAT CTTTTCGCGG ATAAGCAACC TACACATCTT GCCAACAGTA 
AGCCCGGGAT CTCACTACGA CTTCAAGATG TATGCCAATG CCAATGGAGT GGGCTTTGAG 
TCGCGCTTTT TAGGCTCCGT CATTGATAAT GTGGAGAAGA AAAGGGAGCG CTACGAATTC 
TTGCTTTCAT ACATTGGGTT CCTGCGAGCG TACTCCAACC TGAAACGGAA TCGCCAAATC 
CAGATGGAGA TTCCCGGCCT TATATTCCTG TTAAAGGATG TTTTCCCGCA CTTGCACACC 
TGGCACTTTA GCTCTCAAGT GGAGAGAAAC AAGATCTACT TCGAGATTCT TAGCTTTATA 
TGCGACATCC TGGACTTGTT TAACACTGCT AAAGAATCTA ATTGCAAGCA ACGCGAACTT 
TTGGTAAAGG TGTGCGTCTA TTCCCTTCTA AATTTGGAAA ATGGTCTGAT TCTTTTGAGA 
TTTGTTGGTG TGGGCAATGC TTATGTCCAG TACACCATGG AGCTAGAGAC AAATTGGATG 
CAGCAGCAAC CTCACGGCTT AATGATGTTA GTCCGTCTAT CCATGCGTAT CCTGATGCAG 
TTACTGCGCC TTAAAGAAGA GGTATATGGA AACAGTGAGA CCCTTTCCCC GCTGGAGGCG 
TTGATATACA CACAACCCAA ACAGCGTGAT ACTCTGCGGA TCATACCTAC AGTATGCAGT 
TACATGAGCA ACATATTTGA TAGATGGCTA CCGATTCTGT CTTGCCGCCT GCTGAAAAGA 
ATCGCTCTCC AGTTCAATAT GTCTCTCTTG GCCTGTCTGG ACATGGAGGC TGATCAAATC 
CGATTGACGT TTATGCAGAA GTTGCCTGAT GAGCTAGAAA GTGATTCCAT AAAGATAGCC 
ATCCTTGAGC TGGTGGACGC ATGCATTGCC AAGCAACCGG GTGTTACCGA GGCATTTTTC 
AAGGTTAACT ATGCCCTTGA TAAACGTTCC CGGTCATTTT TCAGTAAGGA TTGTGTTCCC 
AATATTGGCG AGAGTATTGT CACTTATATG AGAGACTTTC TGGACGCCCT TCAAGTGGAT 
CCATTAACCA TTCAACAGGC CTTGCCCGCT AAGATCATGA CCATCTTCCA CTCAATGTGG 
AAACATAATC TGCAAATGCT AGTGGATGAT CTACTTCTAA ATATTATATC CATTGAAGTC 
TATACGGGAA ATGGAAACAA TGCCGCTTTG CTTGACGTCA TGAATAAGTT CTTTGAACAG 
AAGAACTTTG GACCTTGGCT TAATTACGTC TTTAACATGC CCAAGGTTCC GGCTGTAAAA 
AATTTGAGTT CTTCCGACCA TTTGCCCGAC TGGATCTGTT GCCTGCAGGC TTTTAAGGAT 
CTTATTGTAA TTTTGCTAAA GAAACAGCCA AAGTTCGTGA CCATACCCGA GTCGCAGTTT 
AAGCTAATGG CGCAGAAGTG TCTGGTTGTC CTGGTTGATC GCTCAAATTA TTTGGAGGAC 
ATGCGGCCGT TCATCATACT GGCTGAACTG TATGTATTCA TCTTACTGGA ATTTAAGCAC 
GCGTACACAG ACAGTTTAGA GGAGGAACAG ACACTAATGG ACCTACTGTT GCAACTGATG 
AACCGCATCT GTGCGTGCTA TGAGGATCAG CACGTAAGGG CCAAAGAGGC ATGCCTGGCC 
ATTGTGACCA AGTGCACACA TCTATACACA GACCTGCTAA TCAGGGACTC GTCCATAGCC 
TTGCGTTTTC TCAACTCAGT CGTAGGCATC ATTTGCAGCG AACTGCAGCA CATGGAGAAC 
TCGGTGAGCC TGGAGAAATC GCAGGGCCTC AATAATTCGG ACAGTTCAGA CAGCAAGACA 
TCCACCAACT CACTTATTCT CTGCCTGAAT TTGCTAAAGG CTGTCGCTAC CATATTCCAC 
AACGACGGTC CCGGAAACTG GGATCTGCCA TTTGTGTCGG TGCGTTTGTT TCAACGGCTC 
GTGCGCTGTG TGTCTAGAAC CCTTCCATTG TTTAGCAAAC AAGTTCTAAG TGTGCAGCTG 
CTGGATGTGC TCATTGTATT TGCCAAGGGC CACTGTTCAG TTGAGTTTCT GCATTGCGAT 
GTGGGAGAAT ACTTGTGGCT GAAACTGTTG CCTCCCAGAG AACTTTTGCA GTCAAAACAC 
GAGTTTACTA AGACGACGGC TGCGGATGCA GAAGGTTGGA CCGTAGAGCA GTGGTGGCCT 
GTATACGCCA GAGGTATTGA GCTTGTTACC ATCATTTACG AGAAGCACAA GAAGTGCTTT 
CTTGAAGATG CTTTTCAATT TGTGGGCATT CATGCTGTTT TCCTGGAGGA TGCATTGTTA 
TTGAGCAAAC AATCCTTGGA ACCGTCAGCC ATGTACTTAA TCAAGGCTGC TGTTAACTTA 
GTCGCCAGTT TGACGGAGCA CCACAAAGAG TGGAAGCAAG ACAGCGATCT GTCGCTTGCT 
AACCTAATGC GTGCAGTTCA GAGTTTGCTG TGCCACACCT CATCTTTGTT TCACCAGCAA 
AAAAACTTAA AGTGTCTTCT GGCTGGTCGA CGCTCCCAGC TAGAAATTCT GCGCAGCACA 
GAGGCTCTAA TCGTTGACGA TGAACTTATC TCCGCCTGCA ATGACCTCAC GGATATAATA 
ATTTCTTGCG TTAAAGCCTT GCTTAGATTT AGTCCGGATT TAATGGAACT ACTGTGCTGC 
AGTGCATATG AGCCCTCCAA ACACTCAATC TTACTAGACG TTAAGTTTGG TGCACCTAAG 
TTGAACGAAG AGAACTTAAC GCTCACATTT GGAATCGTCC TCAACTTGGT AAACATATAC 
GTAAAGGCCT TGAATATGCA AAATCATGGC TTCAGTGAGG TGCCATTAAA CTCACTGCCC 
AACGTTGAGC ATTCCGGGGA CAACGATGAT CCAGAAGTTT GTGTAGGCAA CCAAACCAAC 
CGCACCTTCT CCAAGCCTCT GTCCAATGTC TCGATTTCAA CCGGAACCTG TCCAGCCAGT 
GAGCTGCTCT CCAACATGGA CGGCCAGCTT TGTTTGCTTG CATTGGAGCA TCTGCTCATG 
CTGGTCGCTT CGCAAGCCAT CTGTATAATT CGCTCACCGA ACCTCGAAAC GCTCTGGAAG 
CAAATCGTGC GTCGGGACAT CAGCAACGAG TTGTTAATCT TCAACGAGTT CGTGCGCCGC 
AAGGTGATAT TAGACTACAA AGAGAACCGT AGTCCCTGGC TACGACGAAA ACACGGGCTC 
TGTAAGCTGA AGTGCGTGGA CCCAGTGAGG AGTTCAAGCA GTTCTTCCCG CAGCTCAGAA 
ATTGTGCGCC GCAGCAACAC AAATAACGAA CTGCGAGTTA ATGTCGTACG CAGACTGCAT 
CTGCAGCAAC AGCAGCGAAC TCCTCCACCG CAAAACTTTG ACATGAGCAG CGATCTGAGT 
CCCATTGCGG CAGCTCAAGG TGCAGCCATG ACGACGAGCT TGGATGGCCG AAAGCGTTTG 
TATCCCGCTC AACAGGCGGG CGACGCGTTC TTGGAGGACG AACTGGCCGC CATCGAACTG 
CAATACTTTC CCCCGCCCAC TGAGCCTGGG TATTGTGAAT TGTCGCAGGT TCAAGTGGTG 
GAGGAGGACT ACCTTCAGTT GATGTCGGCC CTCTTTAATG TAATGCCCCA TTGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610810.2
CDS	108..5627
Translation	MPAVEKSVITDWKRLWPMVSGIHYETPQDTVREELMNVASELQAGVLQFKPKNASSLELGTLLKEKKQEKLLPFTERLQDLLDLESAQCWEILCYYLTQEYRGSASLLTQLISTETNMAKLHEDIRHYYSLERMVVLKIVKNLIVFHQVPNHPYHREYRAVVEKITIPRLRDSYLDQLESLICEVPPRKLMAGECFHSAERLVAWSERNAREINEVLHILLVLAEHLPMGLEQIKRIFAACKQHSFGKMQSYLDDSQPYHQEIIRSLSYSELMLVLKCLDFEKPEKHSDLIEKLIEDLQVDIASMYHRPEHGPLLLAWMLLRLRGTNDADDASSLLRCRQLGKRAVDLKCFVQLHLIARHSMYADDSMLSRIVRRTIYNQVGYLCDLFDGDGSCARYEGIYELLCELVSWPHLAKDFCSREDDGPCSLYKTLLENFPLELTHLSKLALSLTKAGQGNYVKSQLEALPILALRYDESQHKLREVDTNEFELLASVQPFQQIDFTIPAGTSCTAIQHPSGCFMHFRFPVNYFDALHHEINCLLRETGHLHGDFESSERIRNVEAGLRFLESAVKLSQSISGISAEMVHPTEMCVDLLHTFKSVQYPPVGLLSSCLNVCTALLPLVDEEIFSRISNLHILPTVSPGSHYDFKMYANANGVGFESRFLGSVIDNVEKKRERYEFLLSYIGFLRAYSNLKRNRQIQMEIPGLIFLLKDVFPHLHTWHFSSQVERNKIYFEILSFICDILDLFNTAKESNCKQRELLVKVCVYSLLNLENGLILLRFVGVGNAYVQYTMELETNWMQQQPHGLMMLVRLSMRILMQLLRLKEEVYGNSETLSPLEALIYTQPKQRDTLRIIPTVCSYMSNIFDRWLPILSCRLLKRIALQFNMSLLACLDMEADQIRLTFMQKLPDELESDSIKIAILELVDACIAKQPGVTEAFFKVNYALDKRSRSFFSKDCVPNIGESIVTYMRDFLDALQVDPLTIQQALPAKIMTIFHSMWKHNLQMLVDDLLLNIISIEVYTGNGNNAALLDVMNKFFEQKNFGPWLNYVFNMPKVPAVKNLSSSDHLPDWICCLQAFKDLIVILLKKQPKFVTIPESQFKLMAQKCLVVLVDRSNYLEDMRPFIILAELYVFILLEFKHAYTDSLEEEQTLMDLLLQLMNRICACYEDQHVRAKEACLAIVTKCTHLYTDLLIRDSSIALRFLNSVVGIICSELQHMENSVSLEKSQGLNNSDSSDSKTSTNSLILCLNLLKAVATIFHNDGPGNWDLPFVSVRLFQRLVRCVSRTLPLFSKQVLSVQLLDVLIVFAKGHCSVEFLHCDVGEYLWLKLLPPRELLQSKHEFTKTTAADAEGWTVEQWWPVYARGIELVTIIYEKHKKCFLEDAFQFVGIHAVFLEDALLLSKQSLEPSAMYLIKAAVNLVASLTEHHKEWKQDSDLSLANLMRAVQSLLCHTSSLFHQQKNLKCLLAGRRSQLEILRSTEALIVDDELISACNDLTDIIISCVKALLRFSPDLMELLCCSAYEPSKHSILLDVKFGAPKLNEENLTLTFGIVLNLVNIYVKALNMQNHGFSEVPLNSLPNVEHSGDNDDPEVCVGNQTNRTFSKPLSNVSISTGTCPASELLSNMDGQLCLLALEHLLMLVASQAICIIRSPNLETLWKQIVRRDISNELLIFNEFVRRKVILDYKENRSPWLRRKHGLCKLKCVDPVRSSSSSSRSSEIVRRSNTNNELRVNVVRRLHLQQQQRTPPPQNFDMSSDLSPIAAAQGAAMTTSLDGRKRLYPAQQAGDAFLEDELAAIELQYFPPPTEPGYCELSQVQVVEEDYLQLMSALFNVMPHCD

Target ORF information:

RefSeq Version	NM_136966.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 188kDa, transcript variant B (Nup188), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136966.4

ORF Insert Sequence:

ATGCCTGCGG TGGAAAAGAG CGTGATCACC GATTGGAAGC GCCTGTGGCC GATGGTCTCA 
GGAATCCACT ATGAGACGCC GCAGGATACT GTACGTGAGG AGCTGATGAA CGTGGCTTCT 
GAATTGCAGG CTGGAGTGCT CCAATTTAAG CCCAAGAATG CCTCCAGCCT CGAGCTGGGC 
ACGTTGCTCA AAGAGAAAAA GCAGGAAAAG CTGCTGCCCT TCACCGAACG ACTGCAGGAT 
CTGTTGGATC TAGAATCCGC CCAGTGCTGG GAAATCCTTT GCTACTACCT TACGCAGGAG 
TACCGTGGAT CGGCGAGTCT CCTTACCCAA CTAATCTCTA CCGAAACGAA TATGGCCAAA 
CTGCACGAAG ACATACGGCA TTACTACTCC CTGGAACGCA TGGTTGTCCT CAAGATTGTT 
AAGAATCTTA TCGTCTTTCA CCAGGTACCG AATCATCCCT ATCACCGGGA GTATCGCGCC 
GTGGTCGAAA AGATAACCAT CCCCCGCCTT AGAGATTCAT ACCTAGACCA ACTGGAGAGC 
CTTATTTGCG AAGTCCCACC CAGAAAGCTA ATGGCGGGAG AATGCTTCCA CTCTGCGGAA 
CGCTTAGTTG CCTGGTCGGA GCGAAATGCT CGTGAGATCA ACGAGGTGCT GCACATCCTT 
CTGGTGCTAG CGGAACACCT GCCCATGGGG TTGGAGCAGA TAAAGAGAAT ATTTGCCGCC 
TGCAAGCAAC ACTCCTTTGG AAAGATGCAA AGTTATCTGG ATGACAGCCA ACCTTACCAT 
CAGGAGATTA TTCGAAGCCT TTCTTACTCA GAGCTAATGT TAGTTTTAAA GTGCCTCGAC 
TTTGAGAAGC CCGAGAAGCA TTCCGATCTA ATTGAAAAGC TTATAGAGGA TCTGCAGGTT 
GATATTGCCA GCATGTACCA CAGACCAGAG CATGGCCCTT TGCTCCTAGC GTGGATGCTG 
CTTCGCTTGC GAGGCACCAA CGATGCCGAC GATGCCTCCA GCTTGCTCCG CTGTCGGCAG 
CTGGGCAAGC GAGCCGTGGA TCTTAAGTGT TTTGTCCAAC TGCACCTAAT TGCCAGACAT 
TCTATGTACG CCGATGACTC TATGCTGTCA CGGATTGTGC GAAGAACCAT ATACAACCAG 
GTTGGATATC TGTGCGACCT CTTCGACGGC GATGGCAGCT GCGCTCGGTA TGAGGGCATT 
TACGAATTGC TCTGCGAACT TGTCTCATGG CCACACTTAG CTAAAGACTT TTGCAGTCGG 
GAAGATGATG GTCCGTGTTC GCTGTACAAA ACTCTTTTAG AGAACTTCCC TCTAGAATTA 
ACACATCTGT CCAAACTGGC ACTGTCACTG ACAAAAGCGG GACAAGGCAA CTATGTCAAG 
AGCCAACTAG AGGCTTTGCC CATATTGGCT CTTCGTTACG ACGAAAGTCA GCACAAACTC 
AGAGAAGTGG ACACAAATGA ATTCGAGCTC CTGGCCAGCG TGCAACCATT TCAGCAAATT 
GATTTTACCA TACCCGCAGG AACTAGTTGC ACAGCCATCC AACATCCGTC CGGCTGCTTT 
ATGCACTTTC GTTTCCCGGT AAACTACTTT GACGCGCTGC ACCACGAAAT TAACTGCCTG 
CTACGCGAGA CGGGTCACCT GCATGGTGAC TTTGAATCGA GCGAACGCAT ACGCAATGTA 
GAAGCTGGTT TAAGATTTTT GGAAAGTGCC GTTAAGCTCA GTCAATCGAT ATCCGGCATC 
AGCGCCGAAA TGGTGCATCC TACCGAGATG TGCGTTGATC TGCTGCACAC GTTCAAAAGC 
GTTCAATACC CACCGGTTGG ACTTTTGTCT AGCTGTCTCA ATGTTTGCAC TGCGCTGCTG 
CCGTTGGTAG ACGAGGAAAT CTTTTCGCGG ATAAGCAACC TACACATCTT GCCAACAGTA 
AGCCCGGGAT CTCACTACGA CTTCAAGATG TATGCCAATG CCAATGGAGT GGGCTTTGAG 
TCGCGCTTTT TAGGCTCCGT CATTGATAAT GTGGAGAAGA AAAGGGAGCG CTACGAATTC 
TTGCTTTCAT ACATTGGGTT CCTGCGAGCG TACTCCAACC TGAAACGGAA TCGCCAAATC 
CAGATGGAGA TTCCCGGCCT TATATTCCTG TTAAAGGATG TTTTCCCGCA CTTGCACACC 
TGGCACTTTA GCTCTCAAGT GGAGAGAAAC AAGATCTACT TCGAGATTCT TAGCTTTATA 
TGCGACATCC TGGACTTGTT TAACACTGCT AAAGAATCTA ATTGCAAGCA ACGCGAACTT 
TTGGTAAAGG TGTGCGTCTA TTCCCTTCTA AATTTGGAAA ATGGTCTGAT TCTTTTGAGA 
TTTGTTGGTG TGGGCAATGC TTATGTCCAG TACACCATGG AGCTAGAGAC AAATTGGATG 
CAGCAGCAAC CTCACGGCTT AATGATGTTA GTCCGTCTAT CCATGCGTAT CCTGATGCAG 
TTACTGCGCC TTAAAGAAGA GGTATATGGA AACAGTGAGA CCCTTTCCCC GCTGGAGGCG 
TTGATATACA CACAACCCAA ACAGCGTGAT ACTCTGCGGA TCATACCTAC AGTATGCAGT 
TACATGAGCA ACATATTTGA TAGATGGCTA CCGATTCTGT CTTGCCGCCT GCTGAAAAGA 
ATCGCTCTCC AGTTCAATAT GTCTCTCTTG GCCTGTCTGG ACATGGAGGC TGATCAAATC 
CGATTGACGT TTATGCAGAA GTTGCCTGAT GAGCTAGAAA GTGATTCCAT AAAGATAGCC 
ATCCTTGAGC TGGTGGACGC ATGCATTGCC AAGCAACCGG GTGTTACCGA GGCATTTTTC 
AAGGTTAACT ATGCCCTTGA TAAACGTTCC CGGTCATTTT TCAGTAAGGA TTGTGTTCCC 
AATATTGGCG AGAGTATTGT CACTTATATG AGAGACTTTC TGGACGCCCT TCAAGTGGAT 
CCATTAACCA TTCAACAGGC CTTGCCCGCT AAGATCATGA CCATCTTCCA CTCAATGTGG 
AAACATAATC TGCAAATGCT AGTGGATGAT CTACTTCTAA ATATTATATC CATTGAAGTC 
TATACGGGAA ATGGAAACAA TGCCGCTTTG CTTGACGTCA TGAATAAGTT CTTTGAACAG 
AAGAACTTTG GACCTTGGCT TAATTACGTC TTTAACATGC CCAAGGTTCC GGCTGTAAAA 
AATTTGAGTT CTTCCGACCA TTTGCCCGAC TGGATCTGTT GCCTGCAGGC TTTTAAGGAT 
CTTATTGTAA TTTTGCTAAA GAAACAGCCA AAGTTCGTGA CCATACCCGA GTCGCAGTTT 
AAGCTAATGG CGCAGAAGTG TCTGGTTGTC CTGGTTGATC GCTCAAATTA TTTGGAGGAC 
ATGCGGCCGT TCATCATACT GGCTGAACTG TATGTATTCA TCTTACTGGA ATTTAAGCAC 
GCGTACACAG ACAGTTTAGA GGAGGAACAG ACACTAATGG ACCTACTGTT GCAACTGATG 
AACCGCATCT GTGCGTGCTA TGAGGATCAG CACGTAAGGG CCAAAGAGGC ATGCCTGGCC 
ATTGTGACCA AGTGCACACA TCTATACACA GACCTGCTAA TCAGGGACTC GTCCATAGCC 
TTGCGTTTTC TCAACTCAGT CGTAGGCATC ATTTGCAGCG AACTGCAGCA CATGGAGAAC 
TCGGTGAGCC TGGAGAAATC GCAGGGCCTC AATAATTCGG ACAGTTCAGA CAGCAAGACA 
TCCACCAACT CACTTATTCT CTGCCTGAAT TTGCTAAAGG CTGTCGCTAC CATATTCCAC 
AACGACGGTC CCGGAAACTG GGATCTGCCA TTTGTGTCGG TGCGTTTGTT TCAACGGCTC 
GTGCGCTGTG TGTCTAGAAC CCTTCCATTG TTTAGCAAAC AAGTTCTAAG TGTGCAGCTG 
CTGGATGTGC TCATTGTATT TGCCAAGGGC CACTGTTCAG TTGAGTTTCT GCATTGCGAT 
GTGGGAGAAT ACTTGTGGCT GAAACTGTTG CCTCCCAGAG AACTTTTGCA GTCAAAACAC 
GAGTTTACTA AGACGACGGC TGCGGATGCA GAAGGTTGGA CCGTAGAGCA GTGGTGGCCT 
GTATACGCCA GAGGTATTGA GCTTGTTACC ATCATTTACG AGAAGCACAA GAAGTGCTTT 
CTTGAAGATG CTTTTCAATT TGTGGGCATT CATGCTGTTT TCCTGGAGGA TGCATTGTTA 
TTGAGCAAAC AATCCTTGGA ACCGTCAGCC ATGTACTTAA TCAAGGCTGC TGTTAACTTA 
GTCGCCAGTT TGACGGAGCA CCACAAAGAG TGGAAGCAAG ACAGCGATCT GTCGCTTGCT 
AACCTAATGC GTGCAGTTCA GAGTTTGCTG TGCCACACCT CATCTTTGTT TCACCAGCAA 
AAAAACTTAA AGTGTCTTCT GGCTGGTCGA CGCTCCCAGC TAGAAATTCT GCGCAGCACA 
GAGGCTCTAA TCGTTGACGA TGAACTTATC TCCGCCTGCA ATGACCTCAC GGATATAATA 
ATTTCTTGCG TTAAAGCCTT GCTTAGATTT AGTCCGGATT TAATGGAACT ACTGTGCTGC 
AGTGCATATG AGCCCTCCAA ACACTCAATC TTACTAGACG TTAAGTTTGG TGCACCTAAG 
TTGAACGAAG AGAACTTAAC GCTCACATTT GGAATCGTCC TCAACTTGGT AAACATATAC 
GTAAAGGCCT TGAATATGCA AAATCATGGC TTCAGTGAGG TGCCATTAAA CTCACTGCCC 
AACGTTGAGC ATTCCGGGGA CAACGATGAT CCAGAAGTTT GTGTAGGCAA CCAAACCAAC 
CGCACCTTCT CCAAGCCTCT GTCCAATGTC TCGATTTCAA CCGGAACCTG TCCAGCCAGT 
GAGCTGCTCT CCAACATGGA CGGCCAGCTT TGTTTGCTTG CATTGGAGCA TCTGCTCATG 
CTGGTCGCTT CGCAAGCCAT CTGTATAATT CGCTCACCGA ACCTCGAAAC GCTCTGGAAG 
CAAATCGTGC GTCGGGACAT CAGCAACGAG TTGTTAATCT TCAACGAGTT CGTGCGCCGC 
AAGGTGATAT TAGACTACAA AGAGAACCGT AGTCCCTGGC TACGACGAAA ACACGGGCTC 
TGTAAGCTGA AGTGCGTGGA CCCAGTGAGG AGTTCAAGCA GTTCTTCCCG CAGCTCAGAA 
ATTGTGCGCC GCAGCAACAC AAATAACGAA CTGCGAGTTA ATGTCGTACG CAGACTGCAT 
CTGCAGCAAC AGCAGCGAAC TCCTCCACCG CAAAACTTTG ACATGAGCAG CGATCTGAGT 
CCCATTGCGG CAGCTCAAGG TGCAGCCATG ACGACGAGCT TGGATGGCCG AAAGCGTTTG 
TATCCCGCTC AACAGGCGGG CGACGCGTTC TTGGAGGACG AACTGGCCGC CATCGAACTG 
CAATACTTTC CCCCGCCCAC TGAGCCTGGG TATTGTGAAT TGTCGCAGGT TCAAGTGGTG 
GAGGAGGACT ACCTTCAGTT GATGTCGGCC CTCTTTAATG TAATGCCCCA TTGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup188 ( NM_001259354.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246283.1 Drosophila melanogaster nucleoporin 188kDa, transcript variant C (Nup188), mRNA.

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CloneID	OFa12176
Clone ID Related Accession (Same CDS sequence)	NM_001259354.2
Accession Version	NM_001259354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 188kDa, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259354.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTGCGG TGGAAAAGAG CGTGATCACC GATTGGAAGC GCCTGTGGCC GATGGTCTCA 
GGAATCCACT ATGAGACGCC GCAGGATACT GTACGTGAGG AGCTGATGAA CGTGGCTTCT 
GAATTGCAGG CTGGAGTGCT CCAATTTAAG CCCAAGAATG CCTCCAGCCT CGAGCTGGGC 
ACGTTGCTCA AAGAGAAAAA GCAGGAAAAG CTGCTGCCCT TCACCGAACG ACTGCAGGAT 
CTGTTGGATC TAGAATCCGC CCAGTGCTGG GAAATCCTTT GCTACTACCT TACGCAGGAG 
TACCGTGGAT CGGCGAGTCT CCTTACCCAA CTAATCTCTA CCGAAACGAA TATGGCCAAA 
CTGCACGAAG ACATACGGCA TTACTACTCC CTGGAACGCA TGGTTGTCCT CAAGATTGTT 
AAGAATCTTA TCGTCTTTCA CCAGGTACCG AATCATCCCT ATCACCGGGA GTATCGCGCC 
GTGGTCGAAA AGATAACCAT CCCCCGCCTT AGAGATTCAT ACCTAGACCA ACTGGAGAGC 
CTTATTTGCG AAGTCCCACC CAGAAAGCTA ATGGCGGGAG AATGCTTCCA CTCTGCGGAA 
CGCTTAGTTG CCTGGTCGGA GCGAAATGCT CGTGAGATCA ACGAGGTGCT GCACATCCTT 
CTGGTGCTAG CGGAACACCT GCCCATGGGG TTGGAGCAGA TAAAGAGAAT ATTTGCCGCC 
TGCAAGCAAC ACTCCTTTGG AAAGATGCAA AGTTATCTGG ATGACAGCCA ACCTTACCAT 
CAGGAGATTA TTCGAAGCCT TTCTTACTCA GAGCTAATGT TAGTTTTAAA GTGCCTCGAC 
TTTGAGAAGC CCGAGAAGCA TTCCGATCTA ATTGAAAAGC TTATAGAGGA TCTGCAGGTT 
GATATTGCCA GCATGTACCA CAGACCAGAG CATGGCCCTT TGCTCCTAGC GTGGATGCTG 
CTTCGCTTGC GAGGCACCAA CGATGCCGAC GATGCCTCCA GCTTGCTCCG CTGTCGGCAG 
CTGGGCAAGC GAGCCGTGGA TCTTAAGTGT TTTGTCCAAC TGCACCTAAT TGCCAGACAT 
TCTATGTACG CCGATGACTC TATGCTGTCA CGGATTGTGC GAAGAACCAT ATACAACCAG 
GTTGGATATC TGTGCGACCT CTTCGACGGC GATGGCAGCT GCGCTCGGTA TGAGGGCATT 
TACGAATTGC TCTGCGAACT TGTCTCATGG CCACACTTAG CTAAAGACTT TTGCAGTCGG 
GAAGATGATG GTCCGTGTTC GCTGTACAAA ACTCTTTTAG AGAACTTCCC TCTAGAATTA 
ACACATCTGT CCAAACTGGC ACTGTCACTG ACAAAAGCGG GACAAGGCAA CTATGTCAAG 
AGCCAACTAG AGGCTTTGCC CATATTGGCT CTTCGTTACG ACGAAAGTCA GCACAAACTC 
AGAGAAGTGG ACACAAATGA ATTCGAGCTC CTGGCCAGCG TGCAACCATT TCAGCAAATT 
GATTTTACCA TACCCGCAGG AACTAGTTGC ACAGCCATCC AACATCCGTC CGGCTGCTTT 
ATGCACTTTC GTTTCCCGGT AAACTACTTT GACGCGCTGC ACCACGAAAT TAACTGCCTG 
CTACGCGAGA CGGGTCACCT GCATGGTGAC TTTGAATCGA GCGAACGCAT ACGCAATGTA 
GAAGCTGGTT TAAGATTTTT GGAAAGTGCC GTTAAGCTCA GTCAATCGAT ATCCGGCATC 
AGCGCCGAAA TGGTGCATCC TACCGAGATG TGCGTTGATC TGCTGCACAC GTTCAAAAGC 
GTTCAATACC CACCGGTTGG ACTTTTGTCT AGCTGTCTCA ATGTTTGCAC TGCGCTGCTG 
CCGTTGGTAG ACGAGGAAAT CTTTTCGCGG ATAAGCAACC TACACATCTT GCCAACAGTA 
AGCCCGGGAT CTCACTACGA CTTCAAGATG TATGCCAATG CCAATGGAGT GGGCTTTGAG 
TCGCGCTTTT TAGGCTCCGT CATTGATAAT GTGGAGAAGA AAAGGGAGCG CTACGAATTC 
TTGCTTTCAT ACATTGGGTT CCTGCGAGCG TACTCCAACC TGAAACGGAA TCGCCAAATC 
CAGATGGAGA TTCCCGGCCT TATATTCCTG TTAAAGGATG TTTTCCCGCA CTTGCACACC 
TGGCACTTTA GCTCTCAAGT GGAGAGAAAC AAGATCTACT TCGAGATTCT TAGCTTTATA 
TGCGACATCC TGGACTTGTT TAACACTGCT AAAGAATCTA ATTGCAAGCA ACGCGAACTT 
TTGGTAAAGG TGTGCGTCTA TTCCCTTCTA AATTTGGAAA ATGGTCTGAT TCTTTTGAGA 
TTTGTTGGTG TGGGCAATGC TTATGTCCAG TACACCATGG AGCTAGAGAC AAATTGGATG 
CAGCAGCAAC CTCACGGCTT AATGATGTTA GTCCGTCTAT CCATGCGTAT CCTGATGCAG 
TTACTGCGCC TTAAAGAAGA GGTATATGGA AACAGTGAGA CCCTTTCCCC GCTGGAGGCG 
TTGATATACA CACAACCCAA ACAGCGTGAT ACTCTGCGGA TCATACCTAC AGTATGCAGT 
TACATGAGCA ACATATTTGA TAGATGGCTA CCGATTCTGT CTTGCCGCCT GCTGAAAAGA 
ATCGCTCTCC AGTTCAATAT GTCTCTCTTG GCCTGTCTGG ACATGGAGGC TGATCAAATC 
CGATTGACGT TTATGCAGAA GTTGCCTGAT GAGCTAGAAA GTGATTCCAT AAAGATAGCC 
ATCCTTGAGC TGGTGGACGC ATGCATTGCC AAGCAACCGG GTGTTACCGA GGCATTTTTC 
AAGGTTAACT ATGCCCTTGA TAAACGTTCC CGGTCATTTT TCAGTAAGGA TTGTGTTCCC 
AATATTGGCG AGAGTATTGT CACTTATATG AGAGACTTTC TGGACGCCCT TCAAGTGGAT 
CCATTAACCA TTCAACAGGC CTTGCCCGCT AAGATCATGA CCATCTTCCA CTCAATGTGG 
AAACATAATC TGCAAATGCT AGTGGATGAT CTAGTGAAGG ATAAACAGTT TTGGAAAAAA 
CTCTGTTCGC CGCTCTTTTC CGAGCTACAG CCAAATTTAC GAATCTACAC TCAGCTTCTA 
AATATTATAT CCATTGAAGT CTATACGGGA AATGGAAACA ATGCCGCTTT GCTTGACGTC 
ATGAATAAGT TCTTTGAACA GAAGAACTTT GGACCTTGGC TTAATTACGT CTTTAACATG 
CCCAAGGTTC CGGCTGTAAA AAATTTGAGT TCTTCCGACC ATTTGCCCGA CTGGATCTGT 
TGCCTGCAGG CTTTTAAGGA TCTTATTGTA ATTTTGCTAA AGAAACAGCC AAAGTTCGTG 
ACCATACCCG AGTCGCAGTT TAAGCTAATG GCGCAGAAGT GTCTGGTTGT CCTGGTTGAT 
CGCTCAAATT ATTTGGAGGA CATGCGGCCG TTCATCATAC TGGCTGAACT GTATGTATTC 
ATCTTACTGG AATTTAAGCA CGCGTACACA GACAGTTTAG AGGAGGAACA GACACTAATG 
GACCTACTGT TGCAACTGAT GAACCGCATC TGTGCGTGCT ATGAGGATCA GCACGTAAGG 
GCCAAAGAGG CATGCCTGGC CATTGTGACC AAGTGCACAC ATCTATACAC AGACCTGCTA 
ATCAGGGACT CGTCCATAGC CTTGCGTTTT CTCAACTCAG TCGTAGGCAT CATTTGCAGC 
GAACTGCAGC ACATGGAGAA CTCGGTGAGC CTGGAGAAAT CGCAGGGCCT CAATAATTCG 
GACAGTTCAG ACAGCAAGAC ATCCACCAAC TCACTTATTC TCTGCCTGAA TTTGCTAAAG 
GCTGTCGCTA CCATATTCCA CAACGACGGT CCCGGAAACT GGGATCTGCC ATTTGTGTCG 
GTGCGTTTGT TTCAACGGCT CGTGCGCTGT GTGTCTAGAA CCCTTCCATT GTTTAGCAAA 
CAAGTTCTAA GTGTGCAGCT GCTGGATGTG CTCATTGTAT TTGCCAAGGG CCACTGTTCA 
GTTGAGTTTC TGCATTGCGA TGTGGGAGAA TACTTGTGGC TGAAACTGTT GCCTCCCAGA 
GAACTTTTGC AGTCAAAACA CGAGTTTACT AAGACGACGG CTGCGGATGC AGAAGGTTGG 
ACCGTAGAGC AGTGGTGGCC TGTATACGCC AGAGGTATTG AGCTTGTTAC CATCATTTAC 
GAGAAGCACA AGAAGTGCTT TCTTGAAGAT GCTTTTCAAT TTGTGGGCAT TCATGCTGTT 
TTCCTGGAGG ATGCATTGTT ATTGAGCAAA CAATCCTTGG AACCGTCAGC CATGTACTTA 
ATCAAGGCTG CTGTTAACTT AGTCGCCAGT TTGACGGAGC ACCACAAAGA GTGGAAGCAA 
GACAGCGATC TGTCGCTTGC TAACCTAATG CGTGCAGTTC AGAGTTTGCT GTGCCACACC 
TCATCTTTGT TTCACCAGCA AAAAAACTTA AAGTGTCTTC TGGCTGGTCG ACGCTCCCAG 
CTAGAAATTC TGCGCAGCAC AGAGGCTCTA ATCGTTGACG ATGAACTTAT CTCCGCCTGC 
AATGACCTCA CGGATATAAT AATTTCTTGC GTTAAAGCCT TGCTTAGATT TAGTCCGGAT 
TTAATGGAAC TACTGTGCTG CAGTGCATAT GAGCCCTCCA AACACTCAAT CTTACTAGAC 
GTTAAGTTTG GTGCACCTAA GTTGAACGAA GAGAACTTAA CGCTCACATT TGGAATCGTC 
CTCAACTTGG TAAACATATA CGTAAAGGCC TTGAATATGC AAAATCATGG CTTCAGTGAG 
GTGCCATTAA ACTCACTGCC CAACGTTGAG CATTCCGGGG ACAACGATGA TCCAGAAGTT 
TGTGTAGGCA ACCAAACCAA CCGCACCTTC TCCAAGCCTC TGTCCAATGT CTCGATTTCA 
ACCGGAACCT GTCCAGCCAG TGAGCTGCTC TCCAACATGG ACGGCCAGCT TTGTTTGCTT 
GCATTGGAGC ATCTGCTCAT GCTGGTCGCT TCGCAAGCCA TCTGTATAAT TCGCTCACCG 
AACCTCGAAA CGCTCTGGAA GCAAATCGTG CGTCGGGACA TCAGCAACGA GTTGTTAATC 
TTCAACGAGT TCGTGCGCCG CAAGGTGATA TTAGACTACA AAGAGAACCG TAGTCCCTGG 
CTACGACGAA AACACGGGCT CTGTAAGCTG AAGTGCGTGG ACCCAGTGAG GAGTTCAAGC 
AGTTCTTCCC GCAGCTCAGA AATTGTGCGC CGCAGCAACA CAAATAACGA ACTGCGAGTT 
AATGTCGTAC GCAGACTGCA TCTGCAGCAA CAGCAGCGAA CTCCTCCACC GCAAAACTTT 
GACATGAGCA GCGATCTGAG TCCCATTGCG GCAGCTCAAG GTGCAGCCAT GACGACGAGC 
TTGGATGGCC GAAAGCGTTT GTATCCCGCT CAACAGGCGG GCGACGCGTT CTTGGAGGAC 
GAACTGGCCG CCATCGAACT GCAATACTTT CCCCCGCCCA CTGAGCCTGG GTATTGTGAA 
TTGTCGCAGG TTCAAGTGGT GGAGGAGGAC TACCTTCAGT TGATGTCGGC CCTCTTTAAT 
GTAATGCCCC ATTGCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246283.1
CDS	108..5708
Translation	MPAVEKSVITDWKRLWPMVSGIHYETPQDTVREELMNVASELQAGVLQFKPKNASSLELGTLLKEKKQEKLLPFTERLQDLLDLESAQCWEILCYYLTQEYRGSASLLTQLISTETNMAKLHEDIRHYYSLERMVVLKIVKNLIVFHQVPNHPYHREYRAVVEKITIPRLRDSYLDQLESLICEVPPRKLMAGECFHSAERLVAWSERNAREINEVLHILLVLAEHLPMGLEQIKRIFAACKQHSFGKMQSYLDDSQPYHQEIIRSLSYSELMLVLKCLDFEKPEKHSDLIEKLIEDLQVDIASMYHRPEHGPLLLAWMLLRLRGTNDADDASSLLRCRQLGKRAVDLKCFVQLHLIARHSMYADDSMLSRIVRRTIYNQVGYLCDLFDGDGSCARYEGIYELLCELVSWPHLAKDFCSREDDGPCSLYKTLLENFPLELTHLSKLALSLTKAGQGNYVKSQLEALPILALRYDESQHKLREVDTNEFELLASVQPFQQIDFTIPAGTSCTAIQHPSGCFMHFRFPVNYFDALHHEINCLLRETGHLHGDFESSERIRNVEAGLRFLESAVKLSQSISGISAEMVHPTEMCVDLLHTFKSVQYPPVGLLSSCLNVCTALLPLVDEEIFSRISNLHILPTVSPGSHYDFKMYANANGVGFESRFLGSVIDNVEKKRERYEFLLSYIGFLRAYSNLKRNRQIQMEIPGLIFLLKDVFPHLHTWHFSSQVERNKIYFEILSFICDILDLFNTAKESNCKQRELLVKVCVYSLLNLENGLILLRFVGVGNAYVQYTMELETNWMQQQPHGLMMLVRLSMRILMQLLRLKEEVYGNSETLSPLEALIYTQPKQRDTLRIIPTVCSYMSNIFDRWLPILSCRLLKRIALQFNMSLLACLDMEADQIRLTFMQKLPDELESDSIKIAILELVDACIAKQPGVTEAFFKVNYALDKRSRSFFSKDCVPNIGESIVTYMRDFLDALQVDPLTIQQALPAKIMTIFHSMWKHNLQMLVDDLVKDKQFWKKLCSPLFSELQPNLRIYTQLLNIISIEVYTGNGNNAALLDVMNKFFEQKNFGPWLNYVFNMPKVPAVKNLSSSDHLPDWICCLQAFKDLIVILLKKQPKFVTIPESQFKLMAQKCLVVLVDRSNYLEDMRPFIILAELYVFILLEFKHAYTDSLEEEQTLMDLLLQLMNRICACYEDQHVRAKEACLAIVTKCTHLYTDLLIRDSSIALRFLNSVVGIICSELQHMENSVSLEKSQGLNNSDSSDSKTSTNSLILCLNLLKAVATIFHNDGPGNWDLPFVSVRLFQRLVRCVSRTLPLFSKQVLSVQLLDVLIVFAKGHCSVEFLHCDVGEYLWLKLLPPRELLQSKHEFTKTTAADAEGWTVEQWWPVYARGIELVTIIYEKHKKCFLEDAFQFVGIHAVFLEDALLLSKQSLEPSAMYLIKAAVNLVASLTEHHKEWKQDSDLSLANLMRAVQSLLCHTSSLFHQQKNLKCLLAGRRSQLEILRSTEALIVDDELISACNDLTDIIISCVKALLRFSPDLMELLCCSAYEPSKHSILLDVKFGAPKLNEENLTLTFGIVLNLVNIYVKALNMQNHGFSEVPLNSLPNVEHSGDNDDPEVCVGNQTNRTFSKPLSNVSISTGTCPASELLSNMDGQLCLLALEHLLMLVASQAICIIRSPNLETLWKQIVRRDISNELLIFNEFVRRKVILDYKENRSPWLRRKHGLCKLKCVDPVRSSSSSSRSSEIVRRSNTNNELRVNVVRRLHLQQQQRTPPPQNFDMSSDLSPIAAAQGAAMTTSLDGRKRLYPAQQAGDAFLEDELAAIELQYFPPPTEPGYCELSQVQVVEEDYLQLMSALFNVMPHCD

Target ORF information:

RefSeq Version	NM_001259354.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 188kDa, transcript variant C (Nup188), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259354.2

ORF Insert Sequence:

ATGCCTGCGG TGGAAAAGAG CGTGATCACC GATTGGAAGC GCCTGTGGCC GATGGTCTCA 
GGAATCCACT ATGAGACGCC GCAGGATACT GTACGTGAGG AGCTGATGAA CGTGGCTTCT 
GAATTGCAGG CTGGAGTGCT CCAATTTAAG CCCAAGAATG CCTCCAGCCT CGAGCTGGGC 
ACGTTGCTCA AAGAGAAAAA GCAGGAAAAG CTGCTGCCCT TCACCGAACG ACTGCAGGAT 
CTGTTGGATC TAGAATCCGC CCAGTGCTGG GAAATCCTTT GCTACTACCT TACGCAGGAG 
TACCGTGGAT CGGCGAGTCT CCTTACCCAA CTAATCTCTA CCGAAACGAA TATGGCCAAA 
CTGCACGAAG ACATACGGCA TTACTACTCC CTGGAACGCA TGGTTGTCCT CAAGATTGTT 
AAGAATCTTA TCGTCTTTCA CCAGGTACCG AATCATCCCT ATCACCGGGA GTATCGCGCC 
GTGGTCGAAA AGATAACCAT CCCCCGCCTT AGAGATTCAT ACCTAGACCA ACTGGAGAGC 
CTTATTTGCG AAGTCCCACC CAGAAAGCTA ATGGCGGGAG AATGCTTCCA CTCTGCGGAA 
CGCTTAGTTG CCTGGTCGGA GCGAAATGCT CGTGAGATCA ACGAGGTGCT GCACATCCTT 
CTGGTGCTAG CGGAACACCT GCCCATGGGG TTGGAGCAGA TAAAGAGAAT ATTTGCCGCC 
TGCAAGCAAC ACTCCTTTGG AAAGATGCAA AGTTATCTGG ATGACAGCCA ACCTTACCAT 
CAGGAGATTA TTCGAAGCCT TTCTTACTCA GAGCTAATGT TAGTTTTAAA GTGCCTCGAC 
TTTGAGAAGC CCGAGAAGCA TTCCGATCTA ATTGAAAAGC TTATAGAGGA TCTGCAGGTT 
GATATTGCCA GCATGTACCA CAGACCAGAG CATGGCCCTT TGCTCCTAGC GTGGATGCTG 
CTTCGCTTGC GAGGCACCAA CGATGCCGAC GATGCCTCCA GCTTGCTCCG CTGTCGGCAG 
CTGGGCAAGC GAGCCGTGGA TCTTAAGTGT TTTGTCCAAC TGCACCTAAT TGCCAGACAT 
TCTATGTACG CCGATGACTC TATGCTGTCA CGGATTGTGC GAAGAACCAT ATACAACCAG 
GTTGGATATC TGTGCGACCT CTTCGACGGC GATGGCAGCT GCGCTCGGTA TGAGGGCATT 
TACGAATTGC TCTGCGAACT TGTCTCATGG CCACACTTAG CTAAAGACTT TTGCAGTCGG 
GAAGATGATG GTCCGTGTTC GCTGTACAAA ACTCTTTTAG AGAACTTCCC TCTAGAATTA 
ACACATCTGT CCAAACTGGC ACTGTCACTG ACAAAAGCGG GACAAGGCAA CTATGTCAAG 
AGCCAACTAG AGGCTTTGCC CATATTGGCT CTTCGTTACG ACGAAAGTCA GCACAAACTC 
AGAGAAGTGG ACACAAATGA ATTCGAGCTC CTGGCCAGCG TGCAACCATT TCAGCAAATT 
GATTTTACCA TACCCGCAGG AACTAGTTGC ACAGCCATCC AACATCCGTC CGGCTGCTTT 
ATGCACTTTC GTTTCCCGGT AAACTACTTT GACGCGCTGC ACCACGAAAT TAACTGCCTG 
CTACGCGAGA CGGGTCACCT GCATGGTGAC TTTGAATCGA GCGAACGCAT ACGCAATGTA 
GAAGCTGGTT TAAGATTTTT GGAAAGTGCC GTTAAGCTCA GTCAATCGAT ATCCGGCATC 
AGCGCCGAAA TGGTGCATCC TACCGAGATG TGCGTTGATC TGCTGCACAC GTTCAAAAGC 
GTTCAATACC CACCGGTTGG ACTTTTGTCT AGCTGTCTCA ATGTTTGCAC TGCGCTGCTG 
CCGTTGGTAG ACGAGGAAAT CTTTTCGCGG ATAAGCAACC TACACATCTT GCCAACAGTA 
AGCCCGGGAT CTCACTACGA CTTCAAGATG TATGCCAATG CCAATGGAGT GGGCTTTGAG 
TCGCGCTTTT TAGGCTCCGT CATTGATAAT GTGGAGAAGA AAAGGGAGCG CTACGAATTC 
TTGCTTTCAT ACATTGGGTT CCTGCGAGCG TACTCCAACC TGAAACGGAA TCGCCAAATC 
CAGATGGAGA TTCCCGGCCT TATATTCCTG TTAAAGGATG TTTTCCCGCA CTTGCACACC 
TGGCACTTTA GCTCTCAAGT GGAGAGAAAC AAGATCTACT TCGAGATTCT TAGCTTTATA 
TGCGACATCC TGGACTTGTT TAACACTGCT AAAGAATCTA ATTGCAAGCA ACGCGAACTT 
TTGGTAAAGG TGTGCGTCTA TTCCCTTCTA AATTTGGAAA ATGGTCTGAT TCTTTTGAGA 
TTTGTTGGTG TGGGCAATGC TTATGTCCAG TACACCATGG AGCTAGAGAC AAATTGGATG 
CAGCAGCAAC CTCACGGCTT AATGATGTTA GTCCGTCTAT CCATGCGTAT CCTGATGCAG 
TTACTGCGCC TTAAAGAAGA GGTATATGGA AACAGTGAGA CCCTTTCCCC GCTGGAGGCG 
TTGATATACA CACAACCCAA ACAGCGTGAT ACTCTGCGGA TCATACCTAC AGTATGCAGT 
TACATGAGCA ACATATTTGA TAGATGGCTA CCGATTCTGT CTTGCCGCCT GCTGAAAAGA 
ATCGCTCTCC AGTTCAATAT GTCTCTCTTG GCCTGTCTGG ACATGGAGGC TGATCAAATC 
CGATTGACGT TTATGCAGAA GTTGCCTGAT GAGCTAGAAA GTGATTCCAT AAAGATAGCC 
ATCCTTGAGC TGGTGGACGC ATGCATTGCC AAGCAACCGG GTGTTACCGA GGCATTTTTC 
AAGGTTAACT ATGCCCTTGA TAAACGTTCC CGGTCATTTT TCAGTAAGGA TTGTGTTCCC 
AATATTGGCG AGAGTATTGT CACTTATATG AGAGACTTTC TGGACGCCCT TCAAGTGGAT 
CCATTAACCA TTCAACAGGC CTTGCCCGCT AAGATCATGA CCATCTTCCA CTCAATGTGG 
AAACATAATC TGCAAATGCT AGTGGATGAT CTAGTGAAGG ATAAACAGTT TTGGAAAAAA 
CTCTGTTCGC CGCTCTTTTC CGAGCTACAG CCAAATTTAC GAATCTACAC TCAGCTTCTA 
AATATTATAT CCATTGAAGT CTATACGGGA AATGGAAACA ATGCCGCTTT GCTTGACGTC 
ATGAATAAGT TCTTTGAACA GAAGAACTTT GGACCTTGGC TTAATTACGT CTTTAACATG 
CCCAAGGTTC CGGCTGTAAA AAATTTGAGT TCTTCCGACC ATTTGCCCGA CTGGATCTGT 
TGCCTGCAGG CTTTTAAGGA TCTTATTGTA ATTTTGCTAA AGAAACAGCC AAAGTTCGTG 
ACCATACCCG AGTCGCAGTT TAAGCTAATG GCGCAGAAGT GTCTGGTTGT CCTGGTTGAT 
CGCTCAAATT ATTTGGAGGA CATGCGGCCG TTCATCATAC TGGCTGAACT GTATGTATTC 
ATCTTACTGG AATTTAAGCA CGCGTACACA GACAGTTTAG AGGAGGAACA GACACTAATG 
GACCTACTGT TGCAACTGAT GAACCGCATC TGTGCGTGCT ATGAGGATCA GCACGTAAGG 
GCCAAAGAGG CATGCCTGGC CATTGTGACC AAGTGCACAC ATCTATACAC AGACCTGCTA 
ATCAGGGACT CGTCCATAGC CTTGCGTTTT CTCAACTCAG TCGTAGGCAT CATTTGCAGC 
GAACTGCAGC ACATGGAGAA CTCGGTGAGC CTGGAGAAAT CGCAGGGCCT CAATAATTCG 
GACAGTTCAG ACAGCAAGAC ATCCACCAAC TCACTTATTC TCTGCCTGAA TTTGCTAAAG 
GCTGTCGCTA CCATATTCCA CAACGACGGT CCCGGAAACT GGGATCTGCC ATTTGTGTCG 
GTGCGTTTGT TTCAACGGCT CGTGCGCTGT GTGTCTAGAA CCCTTCCATT GTTTAGCAAA 
CAAGTTCTAA GTGTGCAGCT GCTGGATGTG CTCATTGTAT TTGCCAAGGG CCACTGTTCA 
GTTGAGTTTC TGCATTGCGA TGTGGGAGAA TACTTGTGGC TGAAACTGTT GCCTCCCAGA 
GAACTTTTGC AGTCAAAACA CGAGTTTACT AAGACGACGG CTGCGGATGC AGAAGGTTGG 
ACCGTAGAGC AGTGGTGGCC TGTATACGCC AGAGGTATTG AGCTTGTTAC CATCATTTAC 
GAGAAGCACA AGAAGTGCTT TCTTGAAGAT GCTTTTCAAT TTGTGGGCAT TCATGCTGTT 
TTCCTGGAGG ATGCATTGTT ATTGAGCAAA CAATCCTTGG AACCGTCAGC CATGTACTTA 
ATCAAGGCTG CTGTTAACTT AGTCGCCAGT TTGACGGAGC ACCACAAAGA GTGGAAGCAA 
GACAGCGATC TGTCGCTTGC TAACCTAATG CGTGCAGTTC AGAGTTTGCT GTGCCACACC 
TCATCTTTGT TTCACCAGCA AAAAAACTTA AAGTGTCTTC TGGCTGGTCG ACGCTCCCAG 
CTAGAAATTC TGCGCAGCAC AGAGGCTCTA ATCGTTGACG ATGAACTTAT CTCCGCCTGC 
AATGACCTCA CGGATATAAT AATTTCTTGC GTTAAAGCCT TGCTTAGATT TAGTCCGGAT 
TTAATGGAAC TACTGTGCTG CAGTGCATAT GAGCCCTCCA AACACTCAAT CTTACTAGAC 
GTTAAGTTTG GTGCACCTAA GTTGAACGAA GAGAACTTAA CGCTCACATT TGGAATCGTC 
CTCAACTTGG TAAACATATA CGTAAAGGCC TTGAATATGC AAAATCATGG CTTCAGTGAG 
GTGCCATTAA ACTCACTGCC CAACGTTGAG CATTCCGGGG ACAACGATGA TCCAGAAGTT 
TGTGTAGGCA ACCAAACCAA CCGCACCTTC TCCAAGCCTC TGTCCAATGT CTCGATTTCA 
ACCGGAACCT GTCCAGCCAG TGAGCTGCTC TCCAACATGG ACGGCCAGCT TTGTTTGCTT 
GCATTGGAGC ATCTGCTCAT GCTGGTCGCT TCGCAAGCCA TCTGTATAAT TCGCTCACCG 
AACCTCGAAA CGCTCTGGAA GCAAATCGTG CGTCGGGACA TCAGCAACGA GTTGTTAATC 
TTCAACGAGT TCGTGCGCCG CAAGGTGATA TTAGACTACA AAGAGAACCG TAGTCCCTGG 
CTACGACGAA AACACGGGCT CTGTAAGCTG AAGTGCGTGG ACCCAGTGAG GAGTTCAAGC 
AGTTCTTCCC GCAGCTCAGA AATTGTGCGC CGCAGCAACA CAAATAACGA ACTGCGAGTT 
AATGTCGTAC GCAGACTGCA TCTGCAGCAA CAGCAGCGAA CTCCTCCACC GCAAAACTTT 
GACATGAGCA GCGATCTGAG TCCCATTGCG GCAGCTCAAG GTGCAGCCAT GACGACGAGC 
TTGGATGGCC GAAAGCGTTT GTATCCCGCT CAACAGGCGG GCGACGCGTT CTTGGAGGAC 
GAACTGGCCG CCATCGAACT GCAATACTTT CCCCCGCCCA CTGAGCCTGG GTATTGTGAA 
TTGTCGCAGG TTCAAGTGGT GGAGGAGGAC TACCTTCAGT TGATGTCGGC CCTCTTTAAT 
GTAATGCCCC ATTGCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Nup188 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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