Iswi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Iswi
Entrez Gene ID	36390
Full Name	Imitation SWI
Synonyms	ACF,CG8625,CHRAC,Dmel\CG8625,ISW,ISWI,NURF,NURF 140,NURF-140,Nurf-140,SWI,anon-EP1279744.124,dCHRAC,dISWI,dNURF,iswi,p140,p140/ISWI
General protein information	Preferred Names Imitation SWI Names CG8625 gene product from transcript CG8625-RA CG8625-PA CG8625-PB CG8625-PC ISWI ISWI ATPase Iswi-PA Iswi-PB Iswi-PC imitation SW imitation SWI imitation switch imitation-SWI nucleosome remodeling factor nucleosome remodeling factor - 140kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49B10-49B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_165930.2	NP_725203.1	imitation SWI, isoform B
NM_165931.2	NP_725204.1	imitation SWI, isoform C
NM_078995.2	NP_523719.1	imitation SWI, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	SMARCA1	NP_001269803.1
Canis lupus familiaris (dog)	SMARCA1	XP_005642178.1
Rattus norvegicus (Norway rat)	Smarca1	NP_001258119.1
Drosophila melanogaster (fruit fly)	Iswi	NP_725204.1
Kluyveromyces lactis	KLLA0F24838g	XP_456186.1
Bos taurus (cattle)	SMARCA1	NP_001178471.1
Gallus gallus (chicken)	SMARCA1	XP_420329.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010700	XP_311417.3
Caenorhabditis elegans (roundworm)	isw-1	NP_498468.2
Saccharomyces cerevisiae (baker's yeast)	ISW2	NP_014948.1
Pan troglodytes (chimpanzee)	SMARCA1	XP_003317720.1
	SMARCA1	XP_002806437.1
Mus musculus (house mouse)	Smarca1	NP_444353.3
Eremothecium gossypii	AGOS_AFR537W	NP_986084.1
Xenopus tropicalis (tropical clawed frog)	smarca1	XP_002931866.2
Danio rerio (zebrafish)	LOC559803	NP_001093467.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the role of ISWI in the hnRNPshsromega interaction
Title: ISWI ATP-dependent remodeling of nucleoplasmic ?-speckles in the brain of Drosophila melanogaster.

ISWI enables bi-directional transitions between two fundamentally different forms of chromatin.
Title: A Testis-Specific Chaperone and the Chromatin Remodeler ISWI Mediate Repackaging of the Paternal Genome.

In a purified system ISWI- and CHD1-type nucleosome remodelers have a clamping activity such that they not only generate regularly spaced nucleosome arrays but also generate constant spacing regardless of nucleosome density.
Title: Nucleosome spacing generated by ISWI and CHD1 remodelers is constant regardless of nucleosome density.

The effect of H4 tail at lysine 16 acetylation on ISWI remodelling depends on the precise nature of the substrate.
Title: ISWI remodelling of physiological chromatin fibres acetylated at lysine 16 of histone H4.

Insertion of flexible linkers at strategic sites in ISWI that disrupt a power stroke does not affect nucleosome sliding.
Title: No need for a power stroke in ISWI-mediated nucleosome sliding.

The following Iswi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iswi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09787	NM_165931.2 Latest version!	Drosophila melanogaster imitation SWI, transcript variant C (Iswi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa09787	NM_078995.2 Latest version!	Drosophila melanogaster imitation SWI, transcript variant A (Iswi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa09787	NM_165930.2 Latest version!	Drosophila melanogaster imitation SWI, transcript variant B (Iswi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Iswi ( NM_165930.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725203.1 Drosophila melanogaster imitation SWI, transcript variant B (Iswi), mRNA.

Price & Availability↑

CloneID	OFa09787
Clone ID Related Accession (Same CDS sequence)	NM_165930.2 , NM_165931.2 , NM_078995.2
Accession Version	NM_165930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	imitation SWI, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165930.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAA CAGATACAGC TGCCGTGGAG GCAACCGAAG AGAACTCGAA CGAGACGACT 
TCAGATGCGG CCACCAGTTC ATCCGGTGAA AAGGAGGCTG AGTTCGACAA CAAAATCGAG 
GCTGATCGCA GTAGGCGCTT TGATTTCCTG CTAAAGCAGA CGGAGATATT CACCCACTTC 
ATGACTAACA GCGCTAAGAG TCCCACGAAG CCTAAGGGTA GACCCAAGAA GATCAAAGAC 
AAGGACAAGG AAAAGGATGT GGCCGATCAT CGTCATCGCA AGACAGAGCA GGAGGAGGAT 
GAGGAGTTGC TGGCGGAAGA CTCGGCCACC AAGGAGATCT TTCGCTTCGA TGCCTCACCC 
GCCTACATCA AAAGTGGAGA GATGCGTGAC TACCAGATTC GCGGTCTTAA CTGGATGATT 
TCGCTTTACG AAAATGGTAT CAATGGAATT CTGGCCGATG AAATGGGTCT AGGAAAGACC 
CTGCAGACCA TATCTCTGCT GGGTTACCTC AAGCATTTCA AAAATCAAGC TGGACCACAC 
ATCGTCATCG TGCCAAAGTC AACGCTTCAG AATTGGGTAA ATGAGTTTAA AAAGTGGTGT 
CCTTCCCTCA GAGCCGTCTG CCTTATTGGT GACCAGGACA CCCGTAACAC CTTCATTAGA 
GATGTGCTCA TGCCTGGCGA GTGGGACGTT TGCGTGACCT CCTATGAGAT GTGTATCCGC 
GAGAAGTCTG TATTCAAGAA GTTCAACTGG CGCTATTTGG TCATCGACGA GGCGCATCGT 
ATCAAGAACG AGAAGTCGAA GCTGTCGGAG ATTCTGCGAG AGTTTAAGAC CGCTAATCGT 
CTACTTATCA CGGGTACTCC GCTGCAGAAT AACCTCCACG AGCTGTGGGC CCTGCTTAAT 
TTCCTGCTGC CCGATGTGTT TAATTCGTCA GAGGATTTTG ACGAATGGTT CAACACGAAC 
ACCTGCCTGG GTGACGATGC ATTGATTACG CGTTTGCATG CCGTGCTGAA ACCTTTCCTG 
CTCCGTCGTC TAAAGGCCGA AGTGGAGAAG CGTCTGAAGC CGAAGAAGGA GATGAAAATA 
TTTGTGGGTC TATCCAAGAT GCAACGCGAC TGGTACACCA AGGTGCTGCT TAAGGACATT 
GATGTAGTGA ACGGTGCTGG CAAAGTGGAG AAGATGCGAC TGCAGAACAT CCTTATGCAG 
CTCCGCAAGT GCACCAACCA CCCATATTTG TTTGATGGCG CCGAGCCCGG TCCGCCATAC 
ACCACGGACA CGCATTTGGT GTATAACTCC GGAAAGATGG CTATTCTGGA CAAGCTGCTG 
CCCAAGCTCC AAGAGCAGGG ATCGCGTGTG TTGATTTTCT CACAAATGAC GAGGATGTTG 
GATATCCTCG AGGACTACTG TCACTGGCGC AACTACAACT ATTGCCGCCT GGATGGTCAG 
ACGCCGCACG AAGATCGTAA CAGGCAGATT CAGGAATTTA ACATGGACAA CAGCGCCAAG 
TTTCTCTTCA TGTTGTCCAC CCGAGCCGGT GGTTTGGGTA TCAATTTGGC TACCGCTGAT 
GTTGTCATCA TTTACGACTC GGATTGGAAT CCTCAAATGG ATTTGCAAGC TATGGATCGT 
GCTCATCGTA TTGGTCAAAA GAAGCAAGTG CGCGTTTTCC GGCTGATCAC CGAAAGTACA 
GTGGAGGAGA AGATCGTGGA GAGAGCAGAG GTCAAGCTCC GTCTGGACAA GATGGTCATC 
CAGGGGGGCA GATTGGTTGA CAACCGCTCC AATCAGTTGA ACAAGGATGA AATGCTTAAT 
ATAATCCGTT TTGGAGCTAA CCAAGTGTTC AGCTCTAAGG AGACAGACAT TACCGATGAG 
GACATCGATG TTATTTTGGA GCGCGGTGAG GCCAAGACGG CCGAGCAAAA GGCAGCACTG 
GACAGTCTGG GCGAGAGTTC GCTGCGGACG TTCACAATGG ACACAAACGG CGAGGCAGGA 
ACTTCTTCCG TATATCAATT CGAGGGTGAG GATTGGCGCG AGAAGCAAAA GCTAAATGCG 
CTGGGCAACT GGATCGAGCC ACCGAAGCGT GAACGCAAAG CCAACTATGC TGTGGATGCC 
TATTTCCGCG AGGCTCTCCG TGTCTCCGAA CCCAAGGCAC CGAAGGCTCC CCGCCCACCC 
AAGCAGCCTA TCGTTCAGGA CTTTCAGTTC TTCCCACCCC GTCTGTTTGA GCTGCTCGAC 
CAGGAAATCT ACTATTTCCG CAAGACTGTT GGTTACAAGG TGCCCAAGAA CACGGAACTA 
GGATCGGATG CCACCAAAGT GCAGCGCGAG GAGCAGCGCA AGATCGATGA GGCAGAGCCG 
CTTACCGAGG AAGAGATCCA GGAGAAGGAG AATCTACTCT CACAGGGTTT CACTGCCTGG 
ACCAAGCGCG ATTTCAACCA GTTCATCAAG GCTAACGAAA AGTACGGTCG GGATGATATT 
GACAACATTG CCAAGGACGT GGAGGGCAAG ACTCCGGAGG AGGTTATCGA GTACAACGCC 
GTGTTTTGGG AGCGATGCAC TGAGTTGCAG GATATTGAGC GAATAATGGG ACAGATTGAG 
CGTGGAGAGG GCAAGATTCA ACGGCGATTG TCTATTAAGA AGGCTTTGGA TCAAAAGATG 
TCGCGGTATC GCGCCCCCTT CCATCAGTTG CGGCTGCAAT ATGGTAATAA CAAGGGCAAG 
AATTACACTG AAATAGAGGA TCGCTTTCTG GTATGCATGC TTCACAAGTT GGGCTTTGAC 
AAGGAGAATG TCTACGAAGA GCTGCGAGCG GCTATAAGAG CTTCTCCGCA ATTCCGCTTT 
GACTGGTTCA TCAAATCTAG AACCGCTTTG GAGCTACAGC GTCGCTGCAA CACGTTGATT 
ACCCTTATTG AACGTGAAAA CATTGAGCTG GAAGAGAAAG AACGCGCCGA GAAAAAGAAA 
AAGGCACCAA AGGGCAGCGT GTCCGCTGGA AGTGGAAGTG CCAGCTCGAA CACTCCAGCA 
CCCGCGCCCC AACCGAAGGC CAGTCAAAAG CGGAAGAGCG AGGTGGTGGC AACCAGTTCC 
AACTCGAAAA AGAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725203.1
CDS	167..3250
Translation	MSKTDTAAVEATEENSNETTSDAATSSSGEKEAEFDNKIEADRSRRFDFLLKQTEIFTHFMTNSAKSPTKPKGRPKKIKDKDKEKDVADHRHRKTEQEEDEELLAEDSATKEIFRFDASPAYIKSGEMRDYQIRGLNWMISLYENGINGILADEMGLGKTLQTISLLGYLKHFKNQAGPHIVIVPKSTLQNWVNEFKKWCPSLRAVCLIGDQDTRNTFIRDVLMPGEWDVCVTSYEMCIREKSVFKKFNWRYLVIDEAHRIKNEKSKLSEILREFKTANRLLITGTPLQNNLHELWALLNFLLPDVFNSSEDFDEWFNTNTCLGDDALITRLHAVLKPFLLRRLKAEVEKRLKPKKEMKIFVGLSKMQRDWYTKVLLKDIDVVNGAGKVEKMRLQNILMQLRKCTNHPYLFDGAEPGPPYTTDTHLVYNSGKMAILDKLLPKLQEQGSRVLIFSQMTRMLDILEDYCHWRNYNYCRLDGQTPHEDRNRQIQEFNMDNSAKFLFMLSTRAGGLGINLATADVVIIYDSDWNPQMDLQAMDRAHRIGQKKQVRVFRLITESTVEEKIVERAEVKLRLDKMVIQGGRLVDNRSNQLNKDEMLNIIRFGANQVFSSKETDITDEDIDVILERGEAKTAEQKAALDSLGESSLRTFTMDTNGEAGTSSVYQFEGEDWREKQKLNALGNWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPIVQDFQFFPPRLFELLDQEIYYFRKTVGYKVPKNTELGSDATKVQREEQRKIDEAEPLTEEEIQEKENLLSQGFTAWTKRDFNQFIKANEKYGRDDIDNIAKDVEGKTPEEVIEYNAVFWERCTELQDIERIMGQIERGEGKIQRRLSIKKALDQKMSRYRAPFHQLRLQYGNNKGKNYTEIEDRFLVCMLHKLGFDKENVYEELRAAIRASPQFRFDWFIKSRTALELQRRCNTLITLIERENIELEEKERAEKKKKAPKGSVSAGSGSASSNTPAPAPQPKASQKRKSEVVATSSNSKKKKK

Target ORF information:

RefSeq Version	NM_165930.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster imitation SWI, transcript variant B (Iswi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165930.2

ORF Insert Sequence:

ATGTCCAAAA CAGATACAGC TGCCGTGGAG GCAACCGAAG AGAACTCGAA CGAGACGACT 
TCAGATGCGG CCACCAGTTC ATCCGGTGAA AAGGAGGCTG AGTTCGACAA CAAAATCGAG 
GCTGATCGCA GTAGGCGCTT TGATTTCCTG CTAAAGCAGA CGGAGATATT CACCCACTTC 
ATGACTAACA GCGCTAAGAG TCCCACGAAG CCTAAGGGTA GACCCAAGAA GATCAAAGAC 
AAGGACAAGG AAAAGGATGT GGCCGATCAT CGTCATCGCA AGACAGAGCA GGAGGAGGAT 
GAGGAGTTGC TGGCGGAAGA CTCGGCCACC AAGGAGATCT TTCGCTTCGA TGCCTCACCC 
GCCTACATCA AAAGTGGAGA GATGCGTGAC TACCAGATTC GCGGTCTTAA CTGGATGATT 
TCGCTTTACG AAAATGGTAT CAATGGAATT CTGGCCGATG AAATGGGTCT AGGAAAGACC 
CTGCAGACCA TATCTCTGCT GGGTTACCTC AAGCATTTCA AAAATCAAGC TGGACCACAC 
ATCGTCATCG TGCCAAAGTC AACGCTTCAG AATTGGGTAA ATGAGTTTAA AAAGTGGTGT 
CCTTCCCTCA GAGCCGTCTG CCTTATTGGT GACCAGGACA CCCGTAACAC CTTCATTAGA 
GATGTGCTCA TGCCTGGCGA GTGGGACGTT TGCGTGACCT CCTATGAGAT GTGTATCCGC 
GAGAAGTCTG TATTCAAGAA GTTCAACTGG CGCTATTTGG TCATCGACGA GGCGCATCGT 
ATCAAGAACG AGAAGTCGAA GCTGTCGGAG ATTCTGCGAG AGTTTAAGAC CGCTAATCGT 
CTACTTATCA CGGGTACTCC GCTGCAGAAT AACCTCCACG AGCTGTGGGC CCTGCTTAAT 
TTCCTGCTGC CCGATGTGTT TAATTCGTCA GAGGATTTTG ACGAATGGTT CAACACGAAC 
ACCTGCCTGG GTGACGATGC ATTGATTACG CGTTTGCATG CCGTGCTGAA ACCTTTCCTG 
CTCCGTCGTC TAAAGGCCGA AGTGGAGAAG CGTCTGAAGC CGAAGAAGGA GATGAAAATA 
TTTGTGGGTC TATCCAAGAT GCAACGCGAC TGGTACACCA AGGTGCTGCT TAAGGACATT 
GATGTAGTGA ACGGTGCTGG CAAAGTGGAG AAGATGCGAC TGCAGAACAT CCTTATGCAG 
CTCCGCAAGT GCACCAACCA CCCATATTTG TTTGATGGCG CCGAGCCCGG TCCGCCATAC 
ACCACGGACA CGCATTTGGT GTATAACTCC GGAAAGATGG CTATTCTGGA CAAGCTGCTG 
CCCAAGCTCC AAGAGCAGGG ATCGCGTGTG TTGATTTTCT CACAAATGAC GAGGATGTTG 
GATATCCTCG AGGACTACTG TCACTGGCGC AACTACAACT ATTGCCGCCT GGATGGTCAG 
ACGCCGCACG AAGATCGTAA CAGGCAGATT CAGGAATTTA ACATGGACAA CAGCGCCAAG 
TTTCTCTTCA TGTTGTCCAC CCGAGCCGGT GGTTTGGGTA TCAATTTGGC TACCGCTGAT 
GTTGTCATCA TTTACGACTC GGATTGGAAT CCTCAAATGG ATTTGCAAGC TATGGATCGT 
GCTCATCGTA TTGGTCAAAA GAAGCAAGTG CGCGTTTTCC GGCTGATCAC CGAAAGTACA 
GTGGAGGAGA AGATCGTGGA GAGAGCAGAG GTCAAGCTCC GTCTGGACAA GATGGTCATC 
CAGGGGGGCA GATTGGTTGA CAACCGCTCC AATCAGTTGA ACAAGGATGA AATGCTTAAT 
ATAATCCGTT TTGGAGCTAA CCAAGTGTTC AGCTCTAAGG AGACAGACAT TACCGATGAG 
GACATCGATG TTATTTTGGA GCGCGGTGAG GCCAAGACGG CCGAGCAAAA GGCAGCACTG 
GACAGTCTGG GCGAGAGTTC GCTGCGGACG TTCACAATGG ACACAAACGG CGAGGCAGGA 
ACTTCTTCCG TATATCAATT CGAGGGTGAG GATTGGCGCG AGAAGCAAAA GCTAAATGCG 
CTGGGCAACT GGATCGAGCC ACCGAAGCGT GAACGCAAAG CCAACTATGC TGTGGATGCC 
TATTTCCGCG AGGCTCTCCG TGTCTCCGAA CCCAAGGCAC CGAAGGCTCC CCGCCCACCC 
AAGCAGCCTA TCGTTCAGGA CTTTCAGTTC TTCCCACCCC GTCTGTTTGA GCTGCTCGAC 
CAGGAAATCT ACTATTTCCG CAAGACTGTT GGTTACAAGG TGCCCAAGAA CACGGAACTA 
GGATCGGATG CCACCAAAGT GCAGCGCGAG GAGCAGCGCA AGATCGATGA GGCAGAGCCG 
CTTACCGAGG AAGAGATCCA GGAGAAGGAG AATCTACTCT CACAGGGTTT CACTGCCTGG 
ACCAAGCGCG ATTTCAACCA GTTCATCAAG GCTAACGAAA AGTACGGTCG GGATGATATT 
GACAACATTG CCAAGGACGT GGAGGGCAAG ACTCCGGAGG AGGTTATCGA GTACAACGCC 
GTGTTTTGGG AGCGATGCAC TGAGTTGCAG GATATTGAGC GAATAATGGG ACAGATTGAG 
CGTGGAGAGG GCAAGATTCA ACGGCGATTG TCTATTAAGA AGGCTTTGGA TCAAAAGATG 
TCGCGGTATC GCGCCCCCTT CCATCAGTTG CGGCTGCAAT ATGGTAATAA CAAGGGCAAG 
AATTACACTG AAATAGAGGA TCGCTTTCTG GTATGCATGC TTCACAAGTT GGGCTTTGAC 
AAGGAGAATG TCTACGAAGA GCTGCGAGCG GCTATAAGAG CTTCTCCGCA ATTCCGCTTT 
GACTGGTTCA TCAAATCTAG AACCGCTTTG GAGCTACAGC GTCGCTGCAA CACGTTGATT 
ACCCTTATTG AACGTGAAAA CATTGAGCTG GAAGAGAAAG AACGCGCCGA GAAAAAGAAA 
AAGGCACCAA AGGGCAGCGT GTCCGCTGGA AGTGGAAGTG CCAGCTCGAA CACTCCAGCA 
CCCGCGCCCC AACCGAAGGC CAGTCAAAAG CGGAAGAGCG AGGTGGTGGC AACCAGTTCC 
AACTCGAAAA AGAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iswi ( NM_165931.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725204.1 Drosophila melanogaster imitation SWI, transcript variant C (Iswi), mRNA.

Price & Availability↑

CloneID	OFa09787
Clone ID Related Accession (Same CDS sequence)	NM_165930.2 , NM_165931.2 , NM_078995.2
Accession Version	NM_165931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	imitation SWI, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165931.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAA CAGATACAGC TGCCGTGGAG GCAACCGAAG AGAACTCGAA CGAGACGACT 
TCAGATGCGG CCACCAGTTC ATCCGGTGAA AAGGAGGCTG AGTTCGACAA CAAAATCGAG 
GCTGATCGCA GTAGGCGCTT TGATTTCCTG CTAAAGCAGA CGGAGATATT CACCCACTTC 
ATGACTAACA GCGCTAAGAG TCCCACGAAG CCTAAGGGTA GACCCAAGAA GATCAAAGAC 
AAGGACAAGG AAAAGGATGT GGCCGATCAT CGTCATCGCA AGACAGAGCA GGAGGAGGAT 
GAGGAGTTGC TGGCGGAAGA CTCGGCCACC AAGGAGATCT TTCGCTTCGA TGCCTCACCC 
GCCTACATCA AAAGTGGAGA GATGCGTGAC TACCAGATTC GCGGTCTTAA CTGGATGATT 
TCGCTTTACG AAAATGGTAT CAATGGAATT CTGGCCGATG AAATGGGTCT AGGAAAGACC 
CTGCAGACCA TATCTCTGCT GGGTTACCTC AAGCATTTCA AAAATCAAGC TGGACCACAC 
ATCGTCATCG TGCCAAAGTC AACGCTTCAG AATTGGGTAA ATGAGTTTAA AAAGTGGTGT 
CCTTCCCTCA GAGCCGTCTG CCTTATTGGT GACCAGGACA CCCGTAACAC CTTCATTAGA 
GATGTGCTCA TGCCTGGCGA GTGGGACGTT TGCGTGACCT CCTATGAGAT GTGTATCCGC 
GAGAAGTCTG TATTCAAGAA GTTCAACTGG CGCTATTTGG TCATCGACGA GGCGCATCGT 
ATCAAGAACG AGAAGTCGAA GCTGTCGGAG ATTCTGCGAG AGTTTAAGAC CGCTAATCGT 
CTACTTATCA CGGGTACTCC GCTGCAGAAT AACCTCCACG AGCTGTGGGC CCTGCTTAAT 
TTCCTGCTGC CCGATGTGTT TAATTCGTCA GAGGATTTTG ACGAATGGTT CAACACGAAC 
ACCTGCCTGG GTGACGATGC ATTGATTACG CGTTTGCATG CCGTGCTGAA ACCTTTCCTG 
CTCCGTCGTC TAAAGGCCGA AGTGGAGAAG CGTCTGAAGC CGAAGAAGGA GATGAAAATA 
TTTGTGGGTC TATCCAAGAT GCAACGCGAC TGGTACACCA AGGTGCTGCT TAAGGACATT 
GATGTAGTGA ACGGTGCTGG CAAAGTGGAG AAGATGCGAC TGCAGAACAT CCTTATGCAG 
CTCCGCAAGT GCACCAACCA CCCATATTTG TTTGATGGCG CCGAGCCCGG TCCGCCATAC 
ACCACGGACA CGCATTTGGT GTATAACTCC GGAAAGATGG CTATTCTGGA CAAGCTGCTG 
CCCAAGCTCC AAGAGCAGGG ATCGCGTGTG TTGATTTTCT CACAAATGAC GAGGATGTTG 
GATATCCTCG AGGACTACTG TCACTGGCGC AACTACAACT ATTGCCGCCT GGATGGTCAG 
ACGCCGCACG AAGATCGTAA CAGGCAGATT CAGGAATTTA ACATGGACAA CAGCGCCAAG 
TTTCTCTTCA TGTTGTCCAC CCGAGCCGGT GGTTTGGGTA TCAATTTGGC TACCGCTGAT 
GTTGTCATCA TTTACGACTC GGATTGGAAT CCTCAAATGG ATTTGCAAGC TATGGATCGT 
GCTCATCGTA TTGGTCAAAA GAAGCAAGTG CGCGTTTTCC GGCTGATCAC CGAAAGTACA 
GTGGAGGAGA AGATCGTGGA GAGAGCAGAG GTCAAGCTCC GTCTGGACAA GATGGTCATC 
CAGGGGGGCA GATTGGTTGA CAACCGCTCC AATCAGTTGA ACAAGGATGA AATGCTTAAT 
ATAATCCGTT TTGGAGCTAA CCAAGTGTTC AGCTCTAAGG AGACAGACAT TACCGATGAG 
GACATCGATG TTATTTTGGA GCGCGGTGAG GCCAAGACGG CCGAGCAAAA GGCAGCACTG 
GACAGTCTGG GCGAGAGTTC GCTGCGGACG TTCACAATGG ACACAAACGG CGAGGCAGGA 
ACTTCTTCCG TATATCAATT CGAGGGTGAG GATTGGCGCG AGAAGCAAAA GCTAAATGCG 
CTGGGCAACT GGATCGAGCC ACCGAAGCGT GAACGCAAAG CCAACTATGC TGTGGATGCC 
TATTTCCGCG AGGCTCTCCG TGTCTCCGAA CCCAAGGCAC CGAAGGCTCC CCGCCCACCC 
AAGCAGCCTA TCGTTCAGGA CTTTCAGTTC TTCCCACCCC GTCTGTTTGA GCTGCTCGAC 
CAGGAAATCT ACTATTTCCG CAAGACTGTT GGTTACAAGG TGCCCAAGAA CACGGAACTA 
GGATCGGATG CCACCAAAGT GCAGCGCGAG GAGCAGCGCA AGATCGATGA GGCAGAGCCG 
CTTACCGAGG AAGAGATCCA GGAGAAGGAG AATCTACTCT CACAGGGTTT CACTGCCTGG 
ACCAAGCGCG ATTTCAACCA GTTCATCAAG GCTAACGAAA AGTACGGTCG GGATGATATT 
GACAACATTG CCAAGGACGT GGAGGGCAAG ACTCCGGAGG AGGTTATCGA GTACAACGCC 
GTGTTTTGGG AGCGATGCAC TGAGTTGCAG GATATTGAGC GAATAATGGG ACAGATTGAG 
CGTGGAGAGG GCAAGATTCA ACGGCGATTG TCTATTAAGA AGGCTTTGGA TCAAAAGATG 
TCGCGGTATC GCGCCCCCTT CCATCAGTTG CGGCTGCAAT ATGGTAATAA CAAGGGCAAG 
AATTACACTG AAATAGAGGA TCGCTTTCTG GTATGCATGC TTCACAAGTT GGGCTTTGAC 
AAGGAGAATG TCTACGAAGA GCTGCGAGCG GCTATAAGAG CTTCTCCGCA ATTCCGCTTT 
GACTGGTTCA TCAAATCTAG AACCGCTTTG GAGCTACAGC GTCGCTGCAA CACGTTGATT 
ACCCTTATTG AACGTGAAAA CATTGAGCTG GAAGAGAAAG AACGCGCCGA GAAAAAGAAA 
AAGGCACCAA AGGGCAGCGT GTCCGCTGGA AGTGGAAGTG CCAGCTCGAA CACTCCAGCA 
CCCGCGCCCC AACCGAAGGC CAGTCAAAAG CGGAAGAGCG AGGTGGTGGC AACCAGTTCC 
AACTCGAAAA AGAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725204.1
CDS	211..3294
Translation	MSKTDTAAVEATEENSNETTSDAATSSSGEKEAEFDNKIEADRSRRFDFLLKQTEIFTHFMTNSAKSPTKPKGRPKKIKDKDKEKDVADHRHRKTEQEEDEELLAEDSATKEIFRFDASPAYIKSGEMRDYQIRGLNWMISLYENGINGILADEMGLGKTLQTISLLGYLKHFKNQAGPHIVIVPKSTLQNWVNEFKKWCPSLRAVCLIGDQDTRNTFIRDVLMPGEWDVCVTSYEMCIREKSVFKKFNWRYLVIDEAHRIKNEKSKLSEILREFKTANRLLITGTPLQNNLHELWALLNFLLPDVFNSSEDFDEWFNTNTCLGDDALITRLHAVLKPFLLRRLKAEVEKRLKPKKEMKIFVGLSKMQRDWYTKVLLKDIDVVNGAGKVEKMRLQNILMQLRKCTNHPYLFDGAEPGPPYTTDTHLVYNSGKMAILDKLLPKLQEQGSRVLIFSQMTRMLDILEDYCHWRNYNYCRLDGQTPHEDRNRQIQEFNMDNSAKFLFMLSTRAGGLGINLATADVVIIYDSDWNPQMDLQAMDRAHRIGQKKQVRVFRLITESTVEEKIVERAEVKLRLDKMVIQGGRLVDNRSNQLNKDEMLNIIRFGANQVFSSKETDITDEDIDVILERGEAKTAEQKAALDSLGESSLRTFTMDTNGEAGTSSVYQFEGEDWREKQKLNALGNWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPIVQDFQFFPPRLFELLDQEIYYFRKTVGYKVPKNTELGSDATKVQREEQRKIDEAEPLTEEEIQEKENLLSQGFTAWTKRDFNQFIKANEKYGRDDIDNIAKDVEGKTPEEVIEYNAVFWERCTELQDIERIMGQIERGEGKIQRRLSIKKALDQKMSRYRAPFHQLRLQYGNNKGKNYTEIEDRFLVCMLHKLGFDKENVYEELRAAIRASPQFRFDWFIKSRTALELQRRCNTLITLIERENIELEEKERAEKKKKAPKGSVSAGSGSASSNTPAPAPQPKASQKRKSEVVATSSNSKKKKK

Target ORF information:

RefSeq Version	NM_165931.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster imitation SWI, transcript variant C (Iswi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165931.2

ORF Insert Sequence:

ATGTCCAAAA CAGATACAGC TGCCGTGGAG GCAACCGAAG AGAACTCGAA CGAGACGACT 
TCAGATGCGG CCACCAGTTC ATCCGGTGAA AAGGAGGCTG AGTTCGACAA CAAAATCGAG 
GCTGATCGCA GTAGGCGCTT TGATTTCCTG CTAAAGCAGA CGGAGATATT CACCCACTTC 
ATGACTAACA GCGCTAAGAG TCCCACGAAG CCTAAGGGTA GACCCAAGAA GATCAAAGAC 
AAGGACAAGG AAAAGGATGT GGCCGATCAT CGTCATCGCA AGACAGAGCA GGAGGAGGAT 
GAGGAGTTGC TGGCGGAAGA CTCGGCCACC AAGGAGATCT TTCGCTTCGA TGCCTCACCC 
GCCTACATCA AAAGTGGAGA GATGCGTGAC TACCAGATTC GCGGTCTTAA CTGGATGATT 
TCGCTTTACG AAAATGGTAT CAATGGAATT CTGGCCGATG AAATGGGTCT AGGAAAGACC 
CTGCAGACCA TATCTCTGCT GGGTTACCTC AAGCATTTCA AAAATCAAGC TGGACCACAC 
ATCGTCATCG TGCCAAAGTC AACGCTTCAG AATTGGGTAA ATGAGTTTAA AAAGTGGTGT 
CCTTCCCTCA GAGCCGTCTG CCTTATTGGT GACCAGGACA CCCGTAACAC CTTCATTAGA 
GATGTGCTCA TGCCTGGCGA GTGGGACGTT TGCGTGACCT CCTATGAGAT GTGTATCCGC 
GAGAAGTCTG TATTCAAGAA GTTCAACTGG CGCTATTTGG TCATCGACGA GGCGCATCGT 
ATCAAGAACG AGAAGTCGAA GCTGTCGGAG ATTCTGCGAG AGTTTAAGAC CGCTAATCGT 
CTACTTATCA CGGGTACTCC GCTGCAGAAT AACCTCCACG AGCTGTGGGC CCTGCTTAAT 
TTCCTGCTGC CCGATGTGTT TAATTCGTCA GAGGATTTTG ACGAATGGTT CAACACGAAC 
ACCTGCCTGG GTGACGATGC ATTGATTACG CGTTTGCATG CCGTGCTGAA ACCTTTCCTG 
CTCCGTCGTC TAAAGGCCGA AGTGGAGAAG CGTCTGAAGC CGAAGAAGGA GATGAAAATA 
TTTGTGGGTC TATCCAAGAT GCAACGCGAC TGGTACACCA AGGTGCTGCT TAAGGACATT 
GATGTAGTGA ACGGTGCTGG CAAAGTGGAG AAGATGCGAC TGCAGAACAT CCTTATGCAG 
CTCCGCAAGT GCACCAACCA CCCATATTTG TTTGATGGCG CCGAGCCCGG TCCGCCATAC 
ACCACGGACA CGCATTTGGT GTATAACTCC GGAAAGATGG CTATTCTGGA CAAGCTGCTG 
CCCAAGCTCC AAGAGCAGGG ATCGCGTGTG TTGATTTTCT CACAAATGAC GAGGATGTTG 
GATATCCTCG AGGACTACTG TCACTGGCGC AACTACAACT ATTGCCGCCT GGATGGTCAG 
ACGCCGCACG AAGATCGTAA CAGGCAGATT CAGGAATTTA ACATGGACAA CAGCGCCAAG 
TTTCTCTTCA TGTTGTCCAC CCGAGCCGGT GGTTTGGGTA TCAATTTGGC TACCGCTGAT 
GTTGTCATCA TTTACGACTC GGATTGGAAT CCTCAAATGG ATTTGCAAGC TATGGATCGT 
GCTCATCGTA TTGGTCAAAA GAAGCAAGTG CGCGTTTTCC GGCTGATCAC CGAAAGTACA 
GTGGAGGAGA AGATCGTGGA GAGAGCAGAG GTCAAGCTCC GTCTGGACAA GATGGTCATC 
CAGGGGGGCA GATTGGTTGA CAACCGCTCC AATCAGTTGA ACAAGGATGA AATGCTTAAT 
ATAATCCGTT TTGGAGCTAA CCAAGTGTTC AGCTCTAAGG AGACAGACAT TACCGATGAG 
GACATCGATG TTATTTTGGA GCGCGGTGAG GCCAAGACGG CCGAGCAAAA GGCAGCACTG 
GACAGTCTGG GCGAGAGTTC GCTGCGGACG TTCACAATGG ACACAAACGG CGAGGCAGGA 
ACTTCTTCCG TATATCAATT CGAGGGTGAG GATTGGCGCG AGAAGCAAAA GCTAAATGCG 
CTGGGCAACT GGATCGAGCC ACCGAAGCGT GAACGCAAAG CCAACTATGC TGTGGATGCC 
TATTTCCGCG AGGCTCTCCG TGTCTCCGAA CCCAAGGCAC CGAAGGCTCC CCGCCCACCC 
AAGCAGCCTA TCGTTCAGGA CTTTCAGTTC TTCCCACCCC GTCTGTTTGA GCTGCTCGAC 
CAGGAAATCT ACTATTTCCG CAAGACTGTT GGTTACAAGG TGCCCAAGAA CACGGAACTA 
GGATCGGATG CCACCAAAGT GCAGCGCGAG GAGCAGCGCA AGATCGATGA GGCAGAGCCG 
CTTACCGAGG AAGAGATCCA GGAGAAGGAG AATCTACTCT CACAGGGTTT CACTGCCTGG 
ACCAAGCGCG ATTTCAACCA GTTCATCAAG GCTAACGAAA AGTACGGTCG GGATGATATT 
GACAACATTG CCAAGGACGT GGAGGGCAAG ACTCCGGAGG AGGTTATCGA GTACAACGCC 
GTGTTTTGGG AGCGATGCAC TGAGTTGCAG GATATTGAGC GAATAATGGG ACAGATTGAG 
CGTGGAGAGG GCAAGATTCA ACGGCGATTG TCTATTAAGA AGGCTTTGGA TCAAAAGATG 
TCGCGGTATC GCGCCCCCTT CCATCAGTTG CGGCTGCAAT ATGGTAATAA CAAGGGCAAG 
AATTACACTG AAATAGAGGA TCGCTTTCTG GTATGCATGC TTCACAAGTT GGGCTTTGAC 
AAGGAGAATG TCTACGAAGA GCTGCGAGCG GCTATAAGAG CTTCTCCGCA ATTCCGCTTT 
GACTGGTTCA TCAAATCTAG AACCGCTTTG GAGCTACAGC GTCGCTGCAA CACGTTGATT 
ACCCTTATTG AACGTGAAAA CATTGAGCTG GAAGAGAAAG AACGCGCCGA GAAAAAGAAA 
AAGGCACCAA AGGGCAGCGT GTCCGCTGGA AGTGGAAGTG CCAGCTCGAA CACTCCAGCA 
CCCGCGCCCC AACCGAAGGC CAGTCAAAAG CGGAAGAGCG AGGTGGTGGC AACCAGTTCC 
AACTCGAAAA AGAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iswi ( NM_078995.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523719.1 Drosophila melanogaster imitation SWI, transcript variant A (Iswi), mRNA.

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CloneID	OFa09787
Clone ID Related Accession (Same CDS sequence)	NM_165930.2 , NM_165931.2 , NM_078995.2
Accession Version	NM_078995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	imitation SWI, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078995.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAA CAGATACAGC TGCCGTGGAG GCAACCGAAG AGAACTCGAA CGAGACGACT 
TCAGATGCGG CCACCAGTTC ATCCGGTGAA AAGGAGGCTG AGTTCGACAA CAAAATCGAG 
GCTGATCGCA GTAGGCGCTT TGATTTCCTG CTAAAGCAGA CGGAGATATT CACCCACTTC 
ATGACTAACA GCGCTAAGAG TCCCACGAAG CCTAAGGGTA GACCCAAGAA GATCAAAGAC 
AAGGACAAGG AAAAGGATGT GGCCGATCAT CGTCATCGCA AGACAGAGCA GGAGGAGGAT 
GAGGAGTTGC TGGCGGAAGA CTCGGCCACC AAGGAGATCT TTCGCTTCGA TGCCTCACCC 
GCCTACATCA AAAGTGGAGA GATGCGTGAC TACCAGATTC GCGGTCTTAA CTGGATGATT 
TCGCTTTACG AAAATGGTAT CAATGGAATT CTGGCCGATG AAATGGGTCT AGGAAAGACC 
CTGCAGACCA TATCTCTGCT GGGTTACCTC AAGCATTTCA AAAATCAAGC TGGACCACAC 
ATCGTCATCG TGCCAAAGTC AACGCTTCAG AATTGGGTAA ATGAGTTTAA AAAGTGGTGT 
CCTTCCCTCA GAGCCGTCTG CCTTATTGGT GACCAGGACA CCCGTAACAC CTTCATTAGA 
GATGTGCTCA TGCCTGGCGA GTGGGACGTT TGCGTGACCT CCTATGAGAT GTGTATCCGC 
GAGAAGTCTG TATTCAAGAA GTTCAACTGG CGCTATTTGG TCATCGACGA GGCGCATCGT 
ATCAAGAACG AGAAGTCGAA GCTGTCGGAG ATTCTGCGAG AGTTTAAGAC CGCTAATCGT 
CTACTTATCA CGGGTACTCC GCTGCAGAAT AACCTCCACG AGCTGTGGGC CCTGCTTAAT 
TTCCTGCTGC CCGATGTGTT TAATTCGTCA GAGGATTTTG ACGAATGGTT CAACACGAAC 
ACCTGCCTGG GTGACGATGC ATTGATTACG CGTTTGCATG CCGTGCTGAA ACCTTTCCTG 
CTCCGTCGTC TAAAGGCCGA AGTGGAGAAG CGTCTGAAGC CGAAGAAGGA GATGAAAATA 
TTTGTGGGTC TATCCAAGAT GCAACGCGAC TGGTACACCA AGGTGCTGCT TAAGGACATT 
GATGTAGTGA ACGGTGCTGG CAAAGTGGAG AAGATGCGAC TGCAGAACAT CCTTATGCAG 
CTCCGCAAGT GCACCAACCA CCCATATTTG TTTGATGGCG CCGAGCCCGG TCCGCCATAC 
ACCACGGACA CGCATTTGGT GTATAACTCC GGAAAGATGG CTATTCTGGA CAAGCTGCTG 
CCCAAGCTCC AAGAGCAGGG ATCGCGTGTG TTGATTTTCT CACAAATGAC GAGGATGTTG 
GATATCCTCG AGGACTACTG TCACTGGCGC AACTACAACT ATTGCCGCCT GGATGGTCAG 
ACGCCGCACG AAGATCGTAA CAGGCAGATT CAGGAATTTA ACATGGACAA CAGCGCCAAG 
TTTCTCTTCA TGTTGTCCAC CCGAGCCGGT GGTTTGGGTA TCAATTTGGC TACCGCTGAT 
GTTGTCATCA TTTACGACTC GGATTGGAAT CCTCAAATGG ATTTGCAAGC TATGGATCGT 
GCTCATCGTA TTGGTCAAAA GAAGCAAGTG CGCGTTTTCC GGCTGATCAC CGAAAGTACA 
GTGGAGGAGA AGATCGTGGA GAGAGCAGAG GTCAAGCTCC GTCTGGACAA GATGGTCATC 
CAGGGGGGCA GATTGGTTGA CAACCGCTCC AATCAGTTGA ACAAGGATGA AATGCTTAAT 
ATAATCCGTT TTGGAGCTAA CCAAGTGTTC AGCTCTAAGG AGACAGACAT TACCGATGAG 
GACATCGATG TTATTTTGGA GCGCGGTGAG GCCAAGACGG CCGAGCAAAA GGCAGCACTG 
GACAGTCTGG GCGAGAGTTC GCTGCGGACG TTCACAATGG ACACAAACGG CGAGGCAGGA 
ACTTCTTCCG TATATCAATT CGAGGGTGAG GATTGGCGCG AGAAGCAAAA GCTAAATGCG 
CTGGGCAACT GGATCGAGCC ACCGAAGCGT GAACGCAAAG CCAACTATGC TGTGGATGCC 
TATTTCCGCG AGGCTCTCCG TGTCTCCGAA CCCAAGGCAC CGAAGGCTCC CCGCCCACCC 
AAGCAGCCTA TCGTTCAGGA CTTTCAGTTC TTCCCACCCC GTCTGTTTGA GCTGCTCGAC 
CAGGAAATCT ACTATTTCCG CAAGACTGTT GGTTACAAGG TGCCCAAGAA CACGGAACTA 
GGATCGGATG CCACCAAAGT GCAGCGCGAG GAGCAGCGCA AGATCGATGA GGCAGAGCCG 
CTTACCGAGG AAGAGATCCA GGAGAAGGAG AATCTACTCT CACAGGGTTT CACTGCCTGG 
ACCAAGCGCG ATTTCAACCA GTTCATCAAG GCTAACGAAA AGTACGGTCG GGATGATATT 
GACAACATTG CCAAGGACGT GGAGGGCAAG ACTCCGGAGG AGGTTATCGA GTACAACGCC 
GTGTTTTGGG AGCGATGCAC TGAGTTGCAG GATATTGAGC GAATAATGGG ACAGATTGAG 
CGTGGAGAGG GCAAGATTCA ACGGCGATTG TCTATTAAGA AGGCTTTGGA TCAAAAGATG 
TCGCGGTATC GCGCCCCCTT CCATCAGTTG CGGCTGCAAT ATGGTAATAA CAAGGGCAAG 
AATTACACTG AAATAGAGGA TCGCTTTCTG GTATGCATGC TTCACAAGTT GGGCTTTGAC 
AAGGAGAATG TCTACGAAGA GCTGCGAGCG GCTATAAGAG CTTCTCCGCA ATTCCGCTTT 
GACTGGTTCA TCAAATCTAG AACCGCTTTG GAGCTACAGC GTCGCTGCAA CACGTTGATT 
ACCCTTATTG AACGTGAAAA CATTGAGCTG GAAGAGAAAG AACGCGCCGA GAAAAAGAAA 
AAGGCACCAA AGGGCAGCGT GTCCGCTGGA AGTGGAAGTG CCAGCTCGAA CACTCCAGCA 
CCCGCGCCCC AACCGAAGGC CAGTCAAAAG CGGAAGAGCG AGGTGGTGGC AACCAGTTCC 
AACTCGAAAA AGAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523719.1
CDS	273..3356
Translation	MSKTDTAAVEATEENSNETTSDAATSSSGEKEAEFDNKIEADRSRRFDFLLKQTEIFTHFMTNSAKSPTKPKGRPKKIKDKDKEKDVADHRHRKTEQEEDEELLAEDSATKEIFRFDASPAYIKSGEMRDYQIRGLNWMISLYENGINGILADEMGLGKTLQTISLLGYLKHFKNQAGPHIVIVPKSTLQNWVNEFKKWCPSLRAVCLIGDQDTRNTFIRDVLMPGEWDVCVTSYEMCIREKSVFKKFNWRYLVIDEAHRIKNEKSKLSEILREFKTANRLLITGTPLQNNLHELWALLNFLLPDVFNSSEDFDEWFNTNTCLGDDALITRLHAVLKPFLLRRLKAEVEKRLKPKKEMKIFVGLSKMQRDWYTKVLLKDIDVVNGAGKVEKMRLQNILMQLRKCTNHPYLFDGAEPGPPYTTDTHLVYNSGKMAILDKLLPKLQEQGSRVLIFSQMTRMLDILEDYCHWRNYNYCRLDGQTPHEDRNRQIQEFNMDNSAKFLFMLSTRAGGLGINLATADVVIIYDSDWNPQMDLQAMDRAHRIGQKKQVRVFRLITESTVEEKIVERAEVKLRLDKMVIQGGRLVDNRSNQLNKDEMLNIIRFGANQVFSSKETDITDEDIDVILERGEAKTAEQKAALDSLGESSLRTFTMDTNGEAGTSSVYQFEGEDWREKQKLNALGNWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPIVQDFQFFPPRLFELLDQEIYYFRKTVGYKVPKNTELGSDATKVQREEQRKIDEAEPLTEEEIQEKENLLSQGFTAWTKRDFNQFIKANEKYGRDDIDNIAKDVEGKTPEEVIEYNAVFWERCTELQDIERIMGQIERGEGKIQRRLSIKKALDQKMSRYRAPFHQLRLQYGNNKGKNYTEIEDRFLVCMLHKLGFDKENVYEELRAAIRASPQFRFDWFIKSRTALELQRRCNTLITLIERENIELEEKERAEKKKKAPKGSVSAGSGSASSNTPAPAPQPKASQKRKSEVVATSSNSKKKKK

Target ORF information:

RefSeq Version	NM_078995.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster imitation SWI, transcript variant A (Iswi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078995.2

ORF Insert Sequence:

ATGTCCAAAA CAGATACAGC TGCCGTGGAG GCAACCGAAG AGAACTCGAA CGAGACGACT 
TCAGATGCGG CCACCAGTTC ATCCGGTGAA AAGGAGGCTG AGTTCGACAA CAAAATCGAG 
GCTGATCGCA GTAGGCGCTT TGATTTCCTG CTAAAGCAGA CGGAGATATT CACCCACTTC 
ATGACTAACA GCGCTAAGAG TCCCACGAAG CCTAAGGGTA GACCCAAGAA GATCAAAGAC 
AAGGACAAGG AAAAGGATGT GGCCGATCAT CGTCATCGCA AGACAGAGCA GGAGGAGGAT 
GAGGAGTTGC TGGCGGAAGA CTCGGCCACC AAGGAGATCT TTCGCTTCGA TGCCTCACCC 
GCCTACATCA AAAGTGGAGA GATGCGTGAC TACCAGATTC GCGGTCTTAA CTGGATGATT 
TCGCTTTACG AAAATGGTAT CAATGGAATT CTGGCCGATG AAATGGGTCT AGGAAAGACC 
CTGCAGACCA TATCTCTGCT GGGTTACCTC AAGCATTTCA AAAATCAAGC TGGACCACAC 
ATCGTCATCG TGCCAAAGTC AACGCTTCAG AATTGGGTAA ATGAGTTTAA AAAGTGGTGT 
CCTTCCCTCA GAGCCGTCTG CCTTATTGGT GACCAGGACA CCCGTAACAC CTTCATTAGA 
GATGTGCTCA TGCCTGGCGA GTGGGACGTT TGCGTGACCT CCTATGAGAT GTGTATCCGC 
GAGAAGTCTG TATTCAAGAA GTTCAACTGG CGCTATTTGG TCATCGACGA GGCGCATCGT 
ATCAAGAACG AGAAGTCGAA GCTGTCGGAG ATTCTGCGAG AGTTTAAGAC CGCTAATCGT 
CTACTTATCA CGGGTACTCC GCTGCAGAAT AACCTCCACG AGCTGTGGGC CCTGCTTAAT 
TTCCTGCTGC CCGATGTGTT TAATTCGTCA GAGGATTTTG ACGAATGGTT CAACACGAAC 
ACCTGCCTGG GTGACGATGC ATTGATTACG CGTTTGCATG CCGTGCTGAA ACCTTTCCTG 
CTCCGTCGTC TAAAGGCCGA AGTGGAGAAG CGTCTGAAGC CGAAGAAGGA GATGAAAATA 
TTTGTGGGTC TATCCAAGAT GCAACGCGAC TGGTACACCA AGGTGCTGCT TAAGGACATT 
GATGTAGTGA ACGGTGCTGG CAAAGTGGAG AAGATGCGAC TGCAGAACAT CCTTATGCAG 
CTCCGCAAGT GCACCAACCA CCCATATTTG TTTGATGGCG CCGAGCCCGG TCCGCCATAC 
ACCACGGACA CGCATTTGGT GTATAACTCC GGAAAGATGG CTATTCTGGA CAAGCTGCTG 
CCCAAGCTCC AAGAGCAGGG ATCGCGTGTG TTGATTTTCT CACAAATGAC GAGGATGTTG 
GATATCCTCG AGGACTACTG TCACTGGCGC AACTACAACT ATTGCCGCCT GGATGGTCAG 
ACGCCGCACG AAGATCGTAA CAGGCAGATT CAGGAATTTA ACATGGACAA CAGCGCCAAG 
TTTCTCTTCA TGTTGTCCAC CCGAGCCGGT GGTTTGGGTA TCAATTTGGC TACCGCTGAT 
GTTGTCATCA TTTACGACTC GGATTGGAAT CCTCAAATGG ATTTGCAAGC TATGGATCGT 
GCTCATCGTA TTGGTCAAAA GAAGCAAGTG CGCGTTTTCC GGCTGATCAC CGAAAGTACA 
GTGGAGGAGA AGATCGTGGA GAGAGCAGAG GTCAAGCTCC GTCTGGACAA GATGGTCATC 
CAGGGGGGCA GATTGGTTGA CAACCGCTCC AATCAGTTGA ACAAGGATGA AATGCTTAAT 
ATAATCCGTT TTGGAGCTAA CCAAGTGTTC AGCTCTAAGG AGACAGACAT TACCGATGAG 
GACATCGATG TTATTTTGGA GCGCGGTGAG GCCAAGACGG CCGAGCAAAA GGCAGCACTG 
GACAGTCTGG GCGAGAGTTC GCTGCGGACG TTCACAATGG ACACAAACGG CGAGGCAGGA 
ACTTCTTCCG TATATCAATT CGAGGGTGAG GATTGGCGCG AGAAGCAAAA GCTAAATGCG 
CTGGGCAACT GGATCGAGCC ACCGAAGCGT GAACGCAAAG CCAACTATGC TGTGGATGCC 
TATTTCCGCG AGGCTCTCCG TGTCTCCGAA CCCAAGGCAC CGAAGGCTCC CCGCCCACCC 
AAGCAGCCTA TCGTTCAGGA CTTTCAGTTC TTCCCACCCC GTCTGTTTGA GCTGCTCGAC 
CAGGAAATCT ACTATTTCCG CAAGACTGTT GGTTACAAGG TGCCCAAGAA CACGGAACTA 
GGATCGGATG CCACCAAAGT GCAGCGCGAG GAGCAGCGCA AGATCGATGA GGCAGAGCCG 
CTTACCGAGG AAGAGATCCA GGAGAAGGAG AATCTACTCT CACAGGGTTT CACTGCCTGG 
ACCAAGCGCG ATTTCAACCA GTTCATCAAG GCTAACGAAA AGTACGGTCG GGATGATATT 
GACAACATTG CCAAGGACGT GGAGGGCAAG ACTCCGGAGG AGGTTATCGA GTACAACGCC 
GTGTTTTGGG AGCGATGCAC TGAGTTGCAG GATATTGAGC GAATAATGGG ACAGATTGAG 
CGTGGAGAGG GCAAGATTCA ACGGCGATTG TCTATTAAGA AGGCTTTGGA TCAAAAGATG 
TCGCGGTATC GCGCCCCCTT CCATCAGTTG CGGCTGCAAT ATGGTAATAA CAAGGGCAAG 
AATTACACTG AAATAGAGGA TCGCTTTCTG GTATGCATGC TTCACAAGTT GGGCTTTGAC 
AAGGAGAATG TCTACGAAGA GCTGCGAGCG GCTATAAGAG CTTCTCCGCA ATTCCGCTTT 
GACTGGTTCA TCAAATCTAG AACCGCTTTG GAGCTACAGC GTCGCTGCAA CACGTTGATT 
ACCCTTATTG AACGTGAAAA CATTGAGCTG GAAGAGAAAG AACGCGCCGA GAAAAAGAAA 
AAGGCACCAA AGGGCAGCGT GTCCGCTGGA AGTGGAAGTG CCAGCTCGAA CACTCCAGCA 
CCCGCGCCCC AACCGAAGGC CAGTCAAAAG CGGAAGAGCG AGGTGGTGGC AACCAGTTCC 
AACTCGAAAA AGAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Iswi cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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