Amph cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Amph
Entrez Gene ID	36383
Full Name	Amphiphysin
Synonyms	Amp,CG8604,DAMP,Damp,Dmel\CG8604,amph,dAmp,dAmph,damph
General protein information	Preferred Names Amphiphysin Names CG8604 gene product from transcript CG8604-RA Amph-PA Amph-PB Amph-PC CG8604-PA CG8604-PB CG8604-PC amphiphysin dAmphiphysin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49B7-49B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273991.1	NP_001260920.1	amphiphysin, isoform C
NM_078993.3	NP_523717.1	amphiphysin, isoform A
NM_001273990.1	NP_001260919.1	amphiphysin, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005076	XP_313952.4
Bos taurus (cattle)	BIN1	NP_001040006.1
Danio rerio (zebrafish)	bin1	NP_001004602.1
Drosophila melanogaster (fruit fly)	Amph	NP_001260919.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our study indicates that the H0 helix and the BAR tip are necessary for efficient and organized self-assembly of amphiphysin/N-BAR
Title: Structural insights into the cooperative remodeling of membranes by amphiphysin/BIN1.

Cleavage furrow tips tubule formation requires the BAR domain of amphiphysin.
Title: The BAR domain of amphiphysin is required for cleavage furrow tip-tubule formation during cellularization in Drosophila embryos.

the structure of the Drosophila amphiphysin BAR domain was solved; the universal and minimal BAR domain is a dimerization, membrane-binding, and curvature-sensing module
Title: BAR domains as sensors of membrane curvature: the amphiphysin BAR structure.

This study challenges the notion that synaptic Amphiphysin is involved exclusively in endocytosis and suggests a novel role for this protein in postsynaptic exocytosis
Title: Drosophila amphiphysin functions during synaptic Fasciclin II membrane cycling.

The following Amph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Amph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09780	NM_078993.3 Latest version!	Drosophila melanogaster amphiphysin, transcript variant A (Amph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12171	NM_001273990.1 Latest version!	Drosophila melanogaster amphiphysin, transcript variant B (Amph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12172	NM_001273991.1 Latest version!	Drosophila melanogaster amphiphysin, transcript variant C (Amph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Amph ( NM_078993.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523717.1 Drosophila melanogaster amphiphysin, transcript variant A (Amph), mRNA.

Price & Availability↑

CloneID	OFa09780
Clone ID Related Accession (Same CDS sequence)	NM_078993.3
Accession Version	NM_078993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	amphiphysin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078993.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAA ATAAAGGCAT AATGCTGGCC AAATCTGTTC AAAAACACGC TGGACGTGCC 
AAGGAGAAGA TTCTACAAAA CTTGGGCAAA GTCGATCGCA CTGCGGATGA AATCTTCGAC 
GATCACCTGA ACAACTTCAA TCGCCAGCAG GCGAGTGCCA ACAGATTACA AAAGGAGTTC 
AATAACTACA TAAGATGTGT TCGCGCCGCA CAAGCCGCCT CCAAGACTCT GATGGATTCC 
GTTTGCGAGA TCTACGAGCC ACAATGGAGC GGTTACGATG CCCTGCAAGC ACAAACGGGT 
GCCTCGGAAA GTCTGTGGGC GGACTTTGCG CACAAATTGG GGGATCAAGT CCTCATTCCG 
CTCAACACAT ACACTGGACA GTTTCCCGAA ATGAAGAAAA AAGTAGAGAA GCGCAACCGA 
AAGCTGATTG ATTACGATGG CCAACGACAC TCGTTTCAGA ATCTGCAGGC CAATGCCAAC 
AAGCGCAAAG ATGATGTCAA ACTAACCAAA GGACGCGAGC AGCTGGAGGA AGCTAGACGC 
ACCTACGAAA TCCTGAACAC GGAACTGCAT GACGAGCTGC CCGCCTTGTA TGACTCTAGG 
ATACTGTTTC TGGTCACCAA CTTGCAGACA CTCTTCGCCA CAGAGCAAGT GTTCCACAAC 
GAAACGGCTA AGATTTACTC GGAACTGGAG GCAATCGTCG ACAAATTGGC CACAGAATCG 
CAGCGCGGCT CCAATACGCT ACGCAAGCAA ACAAGCAATC CCATCAAGAC ATCGAGCCCA 
GTGCAGTCGC CAGTGAATAA GTTAAACAAT GCCAACATCA ACAGCAACTA TCAGAATCAA 
ATAACTACAA ACGGTGGCTC CAGTTTGGCA AACAGCCCGA CATCTACCAG CTCGTCGCTG 
CAAGAGCCTC GATTTGATTC AGTTTCTTCA ACACCAGAAC CACGCCCGGA ATCCCCAGCA 
GCAGCACTAG TATCAGCCAC GCCCAGCAGT CCCGTCGAGA ATGGGGTGAC CACCAAATCA 
TTGGAGCGTC CCGAGCTCAG TGGTCTGAAT GCGAGTGCCA AGGCAACCAC AACCACGCAG 
ACATCGCCCA CGGAGGATAA GGCGGTGGTC AGTGAAGCAG TGAAGCCATC GGAAACTGAG 
GGAGCAGCAG TAGCAGCATC TGTGACACCT GCCCCGCCAG CGACACCTGC CCAAATCAAT 
GGCAACAACA ATGAACCCTC GATTGTTAAG GAAGGTGGCA AACAGCCCAA GGAACTGCCA 
TCTACCACAT CCAATGCAGA AGCTGCTGCC GAGGCGGCGG CCAACAATGG CAATTCCATC 
GAGGAGCACA AGCAGAAGAA ACTAGGTAAT GACACAACAG TCACAGCCAC AGAAACAGTA 
ACAGTCACCC AGCACTCAGT TACATCAACA GATACCGATA ATATAGTAAC CATATCAGAT 
ACGAATACAG ATACAGATAC TAAGACCAGC ACAGGCACTA GTCAGAAAGG TAGACCAGTA 
CCAGTAGTTA ATAGGCACAG CGTAAATAAT CTTAATAAGA ATCCGTTTGA GGATGACGAC 
GAACGCATCT ACGAGGTGCC CGCTGATGCC AATACTGCTG ACTTGCCGCC TGGAGTTCTG 
TACCGGGTGA AGGCCACCTA TGGCTACGCC AAGGAGGATG TGGATGAGCT GAGCTTCGAG 
ATTGGTGACC TTATTCGCGT TATTGAGTAC GACGATCCCG AGGATCAGGA GGAAGGCTGG 
CTGATGGGTC AGAAGGAGGG CACCAACGAG AAGGGACTCT TCCCCGCCAA CTTCACGCGT 
CCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523717.1
CDS	397..2205
Translation	MTENKGIMLAKSVQKHAGRAKEKILQNLGKVDRTADEIFDDHLNNFNRQQASANRLQKEFNNYIRCVRAAQAASKTLMDSVCEIYEPQWSGYDALQAQTGASESLWADFAHKLGDQVLIPLNTYTGQFPEMKKKVEKRNRKLIDYDGQRHSFQNLQANANKRKDDVKLTKGREQLEEARRTYEILNTELHDELPALYDSRILFLVTNLQTLFATEQVFHNETAKIYSELEAIVDKLATESQRGSNTLRKQTSNPIKTSSPVQSPVNKLNNANINSNYQNQITTNGGSSLANSPTSTSSSLQEPRFDSVSSTPEPRPESPAAALVSATPSSPVENGVTTKSLERPELSGLNASAKATTTTQTSPTEDKAVVSEAVKPSETEGAAVAASVTPAPPATPAQINGNNNEPSIVKEGGKQPKELPSTTSNAEAAAEAAANNGNSIEEHKQKKLGNDTTVTATETVTVTQHSVTSTDTDNIVTISDTNTDTDTKTSTGTSQKGRPVPVVNRHSVNNLNKNPFEDDDERIYEVPADANTADLPPGVLYRVKATYGYAKEDVDELSFEIGDLIRVIEYDDPEDQEEGWLMGQKEGTNEKGLFPANFTRPI

Target ORF information:

RefSeq Version	NM_078993.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster amphiphysin, transcript variant A (Amph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078993.3

ORF Insert Sequence:

ATGACCGAAA ATAAAGGCAT AATGCTGGCC AAATCTGTTC AAAAACACGC TGGACGTGCC 
AAGGAGAAGA TTCTACAAAA CTTGGGCAAA GTCGATCGCA CTGCGGATGA AATCTTCGAC 
GATCACCTGA ACAACTTCAA TCGCCAGCAG GCGAGTGCCA ACAGATTACA AAAGGAGTTC 
AATAACTACA TAAGATGTGT TCGCGCCGCA CAAGCCGCCT CCAAGACTCT GATGGATTCC 
GTTTGCGAGA TCTACGAGCC ACAATGGAGC GGTTACGATG CCCTGCAAGC ACAAACGGGT 
GCCTCGGAAA GTCTGTGGGC GGACTTTGCG CACAAATTGG GGGATCAAGT CCTCATTCCG 
CTCAACACAT ACACTGGACA GTTTCCCGAA ATGAAGAAAA AAGTAGAGAA GCGCAACCGA 
AAGCTGATTG ATTACGATGG CCAACGACAC TCGTTTCAGA ATCTGCAGGC CAATGCCAAC 
AAGCGCAAAG ATGATGTCAA ACTAACCAAA GGACGCGAGC AGCTGGAGGA AGCTAGACGC 
ACCTACGAAA TCCTGAACAC GGAACTGCAT GACGAGCTGC CCGCCTTGTA TGACTCTAGG 
ATACTGTTTC TGGTCACCAA CTTGCAGACA CTCTTCGCCA CAGAGCAAGT GTTCCACAAC 
GAAACGGCTA AGATTTACTC GGAACTGGAG GCAATCGTCG ACAAATTGGC CACAGAATCG 
CAGCGCGGCT CCAATACGCT ACGCAAGCAA ACAAGCAATC CCATCAAGAC ATCGAGCCCA 
GTGCAGTCGC CAGTGAATAA GTTAAACAAT GCCAACATCA ACAGCAACTA TCAGAATCAA 
ATAACTACAA ACGGTGGCTC CAGTTTGGCA AACAGCCCGA CATCTACCAG CTCGTCGCTG 
CAAGAGCCTC GATTTGATTC AGTTTCTTCA ACACCAGAAC CACGCCCGGA ATCCCCAGCA 
GCAGCACTAG TATCAGCCAC GCCCAGCAGT CCCGTCGAGA ATGGGGTGAC CACCAAATCA 
TTGGAGCGTC CCGAGCTCAG TGGTCTGAAT GCGAGTGCCA AGGCAACCAC AACCACGCAG 
ACATCGCCCA CGGAGGATAA GGCGGTGGTC AGTGAAGCAG TGAAGCCATC GGAAACTGAG 
GGAGCAGCAG TAGCAGCATC TGTGACACCT GCCCCGCCAG CGACACCTGC CCAAATCAAT 
GGCAACAACA ATGAACCCTC GATTGTTAAG GAAGGTGGCA AACAGCCCAA GGAACTGCCA 
TCTACCACAT CCAATGCAGA AGCTGCTGCC GAGGCGGCGG CCAACAATGG CAATTCCATC 
GAGGAGCACA AGCAGAAGAA ACTAGGTAAT GACACAACAG TCACAGCCAC AGAAACAGTA 
ACAGTCACCC AGCACTCAGT TACATCAACA GATACCGATA ATATAGTAAC CATATCAGAT 
ACGAATACAG ATACAGATAC TAAGACCAGC ACAGGCACTA GTCAGAAAGG TAGACCAGTA 
CCAGTAGTTA ATAGGCACAG CGTAAATAAT CTTAATAAGA ATCCGTTTGA GGATGACGAC 
GAACGCATCT ACGAGGTGCC CGCTGATGCC AATACTGCTG ACTTGCCGCC TGGAGTTCTG 
TACCGGGTGA AGGCCACCTA TGGCTACGCC AAGGAGGATG TGGATGAGCT GAGCTTCGAG 
ATTGGTGACC TTATTCGCGT TATTGAGTAC GACGATCCCG AGGATCAGGA GGAAGGCTGG 
CTGATGGGTC AGAAGGAGGG CACCAACGAG AAGGGACTCT TCCCCGCCAA CTTCACGCGT 
CCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amph ( NM_001273990.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260919.1 Drosophila melanogaster amphiphysin, transcript variant B (Amph), mRNA.

Price & Availability↑

CloneID	OFa12171
Clone ID Related Accession (Same CDS sequence)	NM_001273990.1
Accession Version	NM_001273990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	amphiphysin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAA ATAAAGGCAT AATGCTGGCC AAATCTGTTC AAAAACACGC TGGACGTGCC 
AAGGAGAAGA TTCTACAAAA CTTGGGCAAA GTCGATCGCA CTGCGGATGA AATCTTCGAC 
GATCACCTGA ACAACTTCAA TCGCCAGCAG GCGAGTGCCA ACAGATTACA AAAGGAGTTC 
AATAACTACA TAAGATGTGT TCGCGCCGCA CAAGCCGCCT CCAAGACTCT GATGGATTCC 
GTTTGCGAGA TCTACGAGCC ACAATGGAGC GGTTACGATG CCCTGCAAGC ACAAACGGGT 
GCCTCGGAAA GTCTGTGGGC GGACTTTGCG CACAAATTGG GGGATCAAGT CCTCATTCCG 
CTCAACACAT ACACTGGACA GTTTCCCGAA ATGAAGAAAA AAGTAGAGAA GCGCAACCGA 
AAGCTGATTG ATTACGATGG CCAACGACAC TCGTTTCAGA ATCTGCAGGC CAATGCCAAC 
AAGCGCAAAG ATGATGTCAA ACTAACCAAA GGACGCGAGC AGCTGGAGGA AGCTAGACGC 
ACCTACGAAA TCCTGAACAC GGAACTGCAT GACGAGCTGC CCGCCTTGTA TGACTCTAGG 
ATACTGTTTC TGGTCACCAA CTTGCAGACA CTCTTCGCCA CAGAGCAAGT GTTCCACAAC 
GAAACGGCTA AGATTTACTC GGAACTGGAG GCAATCGTCG ACAAATTGGC CACAGAATCG 
CAGCGCGGCT CCAATACGCT ACGCAAGCAA ACAAGCAATC CCATCAAGAC ATCGAGCCCA 
GTGCAGTCGC CAGTGAATAA GTTAAACAAT GCCAACATCA ACAGCAACTA TCAGAATCAA 
ATAACTACAA ACGGTGGCTC CAGTTTGGCA AACAGCCAAC CACGCCCGGA ATCCCCAGCA 
GCAGCACTAG TATCAGCCAC GCCCAGCAGT CCCGTCGAGA ATGGGGTGAC CACCAAATCA 
TTGGAGCGTC CCGAGCTCAG TGGTCTGAAT GCGAGTGCCA AGGCAACCAC AACCACGCAG 
ACATCGCCCA CGGAGGATAA GGCGGTGGTC AGTGAAGCAG TGAAGCCATC GGAAACTGAG 
GGAGCAGCAG TAGCAGCATC TGTGACACCT GCCCCGCCAG CGACACCTGC CCAAATCAAT 
GGCAACAACA ATGAACCCTC GATTGTTAAG GAAGGTGGCA AACAGCCCAA GGAACTGCCA 
TCTACCACAT CCAATGCAGA AGCTGCTGCC GAGGCGGCGG CCAACAATGG CAATTCCATC 
GAGGAGCACA AGCAGAAGAA ACTAGATGCC AATACTGCTG ACTTGCCGCC TGGAGTTCTG 
TACCGGGTGA AGGCCACCTA TGGCTACGCC AAGGAGGATG TGGATGAGCT GAGCTTCGAG 
ATTGGTGACC TTATTCGCGT TATTGAGTAC GACGATCCCG AGGATCAGGA GGAAGGCTGG 
CTGATGGGTC AGAAGGAGGG CACCAACGAG AAGGGACTCT TCCCCGCCAA CTTCACGCGT 
CCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260919.1
CDS	397..1905
Translation	MTENKGIMLAKSVQKHAGRAKEKILQNLGKVDRTADEIFDDHLNNFNRQQASANRLQKEFNNYIRCVRAAQAASKTLMDSVCEIYEPQWSGYDALQAQTGASESLWADFAHKLGDQVLIPLNTYTGQFPEMKKKVEKRNRKLIDYDGQRHSFQNLQANANKRKDDVKLTKGREQLEEARRTYEILNTELHDELPALYDSRILFLVTNLQTLFATEQVFHNETAKIYSELEAIVDKLATESQRGSNTLRKQTSNPIKTSSPVQSPVNKLNNANINSNYQNQITTNGGSSLANSQPRPESPAAALVSATPSSPVENGVTTKSLERPELSGLNASAKATTTTQTSPTEDKAVVSEAVKPSETEGAAVAASVTPAPPATPAQINGNNNEPSIVKEGGKQPKELPSTTSNAEAAAEAAANNGNSIEEHKQKKLDANTADLPPGVLYRVKATYGYAKEDVDELSFEIGDLIRVIEYDDPEDQEEGWLMGQKEGTNEKGLFPANFTRPI

Target ORF information:

RefSeq Version	NM_001273990.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster amphiphysin, transcript variant B (Amph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273990.1

ORF Insert Sequence:

ATGACCGAAA ATAAAGGCAT AATGCTGGCC AAATCTGTTC AAAAACACGC TGGACGTGCC 
AAGGAGAAGA TTCTACAAAA CTTGGGCAAA GTCGATCGCA CTGCGGATGA AATCTTCGAC 
GATCACCTGA ACAACTTCAA TCGCCAGCAG GCGAGTGCCA ACAGATTACA AAAGGAGTTC 
AATAACTACA TAAGATGTGT TCGCGCCGCA CAAGCCGCCT CCAAGACTCT GATGGATTCC 
GTTTGCGAGA TCTACGAGCC ACAATGGAGC GGTTACGATG CCCTGCAAGC ACAAACGGGT 
GCCTCGGAAA GTCTGTGGGC GGACTTTGCG CACAAATTGG GGGATCAAGT CCTCATTCCG 
CTCAACACAT ACACTGGACA GTTTCCCGAA ATGAAGAAAA AAGTAGAGAA GCGCAACCGA 
AAGCTGATTG ATTACGATGG CCAACGACAC TCGTTTCAGA ATCTGCAGGC CAATGCCAAC 
AAGCGCAAAG ATGATGTCAA ACTAACCAAA GGACGCGAGC AGCTGGAGGA AGCTAGACGC 
ACCTACGAAA TCCTGAACAC GGAACTGCAT GACGAGCTGC CCGCCTTGTA TGACTCTAGG 
ATACTGTTTC TGGTCACCAA CTTGCAGACA CTCTTCGCCA CAGAGCAAGT GTTCCACAAC 
GAAACGGCTA AGATTTACTC GGAACTGGAG GCAATCGTCG ACAAATTGGC CACAGAATCG 
CAGCGCGGCT CCAATACGCT ACGCAAGCAA ACAAGCAATC CCATCAAGAC ATCGAGCCCA 
GTGCAGTCGC CAGTGAATAA GTTAAACAAT GCCAACATCA ACAGCAACTA TCAGAATCAA 
ATAACTACAA ACGGTGGCTC CAGTTTGGCA AACAGCCAAC CACGCCCGGA ATCCCCAGCA 
GCAGCACTAG TATCAGCCAC GCCCAGCAGT CCCGTCGAGA ATGGGGTGAC CACCAAATCA 
TTGGAGCGTC CCGAGCTCAG TGGTCTGAAT GCGAGTGCCA AGGCAACCAC AACCACGCAG 
ACATCGCCCA CGGAGGATAA GGCGGTGGTC AGTGAAGCAG TGAAGCCATC GGAAACTGAG 
GGAGCAGCAG TAGCAGCATC TGTGACACCT GCCCCGCCAG CGACACCTGC CCAAATCAAT 
GGCAACAACA ATGAACCCTC GATTGTTAAG GAAGGTGGCA AACAGCCCAA GGAACTGCCA 
TCTACCACAT CCAATGCAGA AGCTGCTGCC GAGGCGGCGG CCAACAATGG CAATTCCATC 
GAGGAGCACA AGCAGAAGAA ACTAGATGCC AATACTGCTG ACTTGCCGCC TGGAGTTCTG 
TACCGGGTGA AGGCCACCTA TGGCTACGCC AAGGAGGATG TGGATGAGCT GAGCTTCGAG 
ATTGGTGACC TTATTCGCGT TATTGAGTAC GACGATCCCG AGGATCAGGA GGAAGGCTGG 
CTGATGGGTC AGAAGGAGGG CACCAACGAG AAGGGACTCT TCCCCGCCAA CTTCACGCGT 
CCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amph ( NM_001273991.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260920.1 Drosophila melanogaster amphiphysin, transcript variant C (Amph), mRNA.

Price & Availability↑

CloneID	OFa12172
Clone ID Related Accession (Same CDS sequence)	NM_001273991.1
Accession Version	NM_001273991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	amphiphysin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAA ATAAAGGCAT AATGCTGGCC AAATCTGTTC AAAAACACGC TGGACGTGCC 
AAGGAGAAGA TTCTACAAAA CTTGGGCAAA GTCGATCGCA CTGCGGATGA AATCTTCGAC 
GATCACCTGA ACAACTTCAA TCGCCAGCAG GCGAGTGCCA ACAGATTACA AAAGGAGTTC 
AATAACTACA TAAGATGTGT TCGCGCCGCA CAAGCCGCCT CCAAGACTCT GATGGATTCC 
GTTTGCGAGA TCTACGAGCC ACAATGGAGC GGTTACGATG CCCTGCAAGC ACAAACGGGT 
GCCTCGGAAA GTCTGTGGGC GGACTTTGCG CACAAATTGG GGGATCAAGT CCTCATTCCG 
CTCAACACAT ACACTGGACA GTTTCCCGAA ATGAAGAAAA AAGTAGAGAA GCGCAACCGA 
AAGCTGATTG ATTACGATGG CCAACGACAC TCGTTTCAGA ATCTGCAGGC CAATGCCAAC 
AAGCGCAAAG ATGATGTCAA ACTAACCAAA GGACGCGAGC AGCTGGAGGA AGCTAGACGC 
ACCTACGAAA TCCTGAACAC GGAACTGCAT GACGAGCTGC CCGCCTTGTA TGACTCTAGG 
ATACTGTTTC TGGTCACCAA CTTGCAGACA CTCTTCGCCA CAGAGCAAGT GTTCCACAAC 
GAAACGGCTA AGATTTACTC GGAACTGGAG GCAATCGTCG ACAAATTGGC CACAGAATCG 
CAGCGCGGCT CCAATACGCT ACGCAAGCAA ACAAGCAATC CCATCAAGAC ATCGAGCCCA 
GTGCAGTCGC CAGTGAATAA GTTAAACAAT GCCAACATCA ACAGCAACTA TCAGAATCAA 
ATAACTACAA ACGGTGGCTC CAGTTTGGCA AACAGCCATG CCAATACTGC TGACTTGCCG 
CCTGGAGTTC TGTACCGGGT GAAGGCCACC TATGGCTACG CCAAGGAGGA TGTGGATGAG 
CTGAGCTTCG AGATTGGTGA CCTTATTCGC GTTATTGAGT ACGACGATCC CGAGGATCAG 
TTCTTTATTG CCAATTTACA GGAGGAAGGC TGGCTGATGG GTCAGAAGGA GGGCACCAAC 
GAGAAGGGAC TCTTCCCCGC CAACTTCACG CGTCCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260920.1
CDS	397..1518
Translation	MTENKGIMLAKSVQKHAGRAKEKILQNLGKVDRTADEIFDDHLNNFNRQQASANRLQKEFNNYIRCVRAAQAASKTLMDSVCEIYEPQWSGYDALQAQTGASESLWADFAHKLGDQVLIPLNTYTGQFPEMKKKVEKRNRKLIDYDGQRHSFQNLQANANKRKDDVKLTKGREQLEEARRTYEILNTELHDELPALYDSRILFLVTNLQTLFATEQVFHNETAKIYSELEAIVDKLATESQRGSNTLRKQTSNPIKTSSPVQSPVNKLNNANINSNYQNQITTNGGSSLANSHANTADLPPGVLYRVKATYGYAKEDVDELSFEIGDLIRVIEYDDPEDQFFIANLQEEGWLMGQKEGTNEKGLFPANFTRPI

Target ORF information:

RefSeq Version	NM_001273991.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster amphiphysin, transcript variant C (Amph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273991.1

ORF Insert Sequence:

ATGACCGAAA ATAAAGGCAT AATGCTGGCC AAATCTGTTC AAAAACACGC TGGACGTGCC 
AAGGAGAAGA TTCTACAAAA CTTGGGCAAA GTCGATCGCA CTGCGGATGA AATCTTCGAC 
GATCACCTGA ACAACTTCAA TCGCCAGCAG GCGAGTGCCA ACAGATTACA AAAGGAGTTC 
AATAACTACA TAAGATGTGT TCGCGCCGCA CAAGCCGCCT CCAAGACTCT GATGGATTCC 
GTTTGCGAGA TCTACGAGCC ACAATGGAGC GGTTACGATG CCCTGCAAGC ACAAACGGGT 
GCCTCGGAAA GTCTGTGGGC GGACTTTGCG CACAAATTGG GGGATCAAGT CCTCATTCCG 
CTCAACACAT ACACTGGACA GTTTCCCGAA ATGAAGAAAA AAGTAGAGAA GCGCAACCGA 
AAGCTGATTG ATTACGATGG CCAACGACAC TCGTTTCAGA ATCTGCAGGC CAATGCCAAC 
AAGCGCAAAG ATGATGTCAA ACTAACCAAA GGACGCGAGC AGCTGGAGGA AGCTAGACGC 
ACCTACGAAA TCCTGAACAC GGAACTGCAT GACGAGCTGC CCGCCTTGTA TGACTCTAGG 
ATACTGTTTC TGGTCACCAA CTTGCAGACA CTCTTCGCCA CAGAGCAAGT GTTCCACAAC 
GAAACGGCTA AGATTTACTC GGAACTGGAG GCAATCGTCG ACAAATTGGC CACAGAATCG 
CAGCGCGGCT CCAATACGCT ACGCAAGCAA ACAAGCAATC CCATCAAGAC ATCGAGCCCA 
GTGCAGTCGC CAGTGAATAA GTTAAACAAT GCCAACATCA ACAGCAACTA TCAGAATCAA 
ATAACTACAA ACGGTGGCTC CAGTTTGGCA AACAGCCATG CCAATACTGC TGACTTGCCG 
CCTGGAGTTC TGTACCGGGT GAAGGCCACC TATGGCTACG CCAAGGAGGA TGTGGATGAG 
CTGAGCTTCG AGATTGGTGA CCTTATTCGC GTTATTGAGT ACGACGATCC CGAGGATCAG 
TTCTTTATTG CCAATTTACA GGAGGAAGGC TGGCTGATGG GTCAGAAGGA GGGCACCAAC 
GAGAAGGGAC TCTTCCCCGC CAACTTCACG CGTCCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Amph cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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