fra cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fra
Entrez Gene ID	36377
Full Name	frazzled
Synonyms	CG8581,CT24981,DCC,Dmel\CG8581,Fra
General protein information	Preferred Names frazzled Names CG8581 gene product from transcript CG8581-RA CG8581-PA CG8581-PB CG8581-PC DCC/Frazzled Netrin-receptor deleted in colorectal cancer deleted in colorectal carcinoma fra-PA fra-PB fra-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49A10-49B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165913.2	NP_725185.1	frazzled, isoform B
NM_078992.3	NP_523716.2	frazzled, isoform A
NM_001299424.1	NP_001286353.1	frazzled, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	neo1	NP_775325.1
Canis lupus familiaris (dog)	NEO1	XP_005638634.1
Bos taurus (cattle)	NEO1	XP_005211488.1
Mus musculus (house mouse)	Neo1	NP_032710.2
Drosophila melanogaster (fruit fly)	fra	NP_725185.1
Homo sapiens (human)	NEO1	NP_002490.2
Pan troglodytes (chimpanzee)	NEO1	XP_510660.3
	NEO1	XP_002804901.1
Rattus norvegicus (Norway rat)	Neo1	XP_006243248.1
Gallus gallus (chicken)	NEO1	XP_004943713.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006083	XP_316139.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Based on findings in other systems, we propose that adhesion to substrate-bound Net underlies both long- and short-range Net-Fra-dependent guidance in vivo, thereby eroding the distinction between them.
Title: Frazzled promotes growth cone attachment at the source of a Netrin gradient in the

Frazzled expression is induced by the transcription factor Glide/Gcm in a dose-dependent manner.
Title: The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled.

Frazzled was found to have a role as a transcription factor whose activity regulates the responsiveness of commissural axons at the midline.
Title: The Intracellular Domain of the Frazzled/DCC Receptor Is a Transcription Factor Required for Commissural Axon Guidance.

Contact-mediated cell adhesion via Fmi acts in addition to the Net/Fra guidance system during axon pathfinding across the midline, underlining the importance of PCP molecules during vertebrates and invertebrates midline development.
Title: Flamingo, a seven-pass transmembrane cadherin, cooperates with Netrin/Frazzled in Drosophila midline guidance.

In peripodial cells Frazzled is endocytosed, and undergoes NetA-dependent degradation, which is required for eversion.
Title: Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila.

The following fra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09771	NM_078992.3 Latest version!	Drosophila melanogaster frazzled (fra), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa09772	NM_165913.2 Latest version!	Drosophila melanogaster frazzled (fra), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa21786	NM_001299424.1 Latest version!	Drosophila melanogaster frazzled (fra), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fra ( NM_078992.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523716.2 Drosophila melanogaster frazzled (fra), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09771
Clone ID Related Accession (Same CDS sequence)	NM_078992.3
Accession Version	NM_078992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frazzled, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078992.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATCA CAACAAACAG AAGCAGCAGG ACTTTGTGGA ACTGGCTGCT CAGCAGTTGT 
CTCATATTCC AGCTGATTGG CAGCAGTTTG GCAAGTCAAG CTCTTTCGTT CACCTTGGAG 
CCCCAGGATG CCGTGGTGCC CGAGGGCCAC TCGGTGCTGC TCCAGTGTGC AGGCACTGCA 
TCCATTGGAC GTGGAGGCAA GAGCAAGTCC AACTTACCCA GCTCAGTGAG CATCCGGTGG 
CGAGGACCTG ATGGGCAGGA CCTGGTCATC GTGGGCGATA CGTTCCGCAC TCAGCTGAAG 
AATGGATCAC TGTACATTAG CTCCGTTGAG GAGAACCGTG GACTGACGGG AGCCTACCAA 
TGCCTGTTGA CTGCCGAGGG CGTGGGCAGC ATACTCAGTC GACCCGCACT GGTGGCCATT 
GTCCGACAGC CAGATCTGAA TCAGGACTTC CTGGAAACCT ACCTGCTGCC AGGTCAGACG 
GCCTACTTCC GTTGCATGCT GGGCGAAGCC AACTGGCAGG AGGGCGTTAA GCATTCGGTA 
CAGTGGCTCA AGGATGACCT GCCACTGCCC CTGGACAAAC TGCGCATGGT GGTGCTGCCC 
AACGGAGCGC TGGAGATCGA CGAAGTGGGT CCATCGGATC GCGGATCCTA TCAGTGCAAC 
GTGACTTCGG GCAGTTCCTC CCGGCTAAGC AGCAAAACGA ACTTGAACAT TAAGAAACCC 
AGTGATCCGG GTGCCGAGAA CTCAGTGGCA CCATCGTTCC TTGTGGGACC TTCGCCCAAA 
ACCGTGAGAG AGGGCGACAC CGTCACCCTG GACTGTGTGG CCAACGGAGT GCCAAAGCCG 
CAGATTAAGT GGCTGCGAAA TGGCATGGAT CTGGATTTTA ACGACCTGGA CTCACGCTTT 
TCCATCGTTG GAACGGGTTC ACTGCAAATC TCCAGTGCCG AGGACATTGA TTCAGGGAAC 
TACCAGTGCC GGGCCAGCAA TACGGTGGAT TCTCTTGATG CTCAGGCCAC GGTGCAGGTA 
CAGGAACCAC CTAAGTTCAT TAAGGCGCCC AAGGATACCA CTGCCCACGA GAAGGACGAA 
CCGGAATTGA AGTGCGATAT CTGGGGAAAG CCAAAGCCCG TAATAAGGTG GCTTAAGAAC 
GGAGATCTCA TAACGCCCAA TGACTACATG CAACTGGTGG ATGGTCACAA TCTGAAGATC 
CTCGGACTTC TCAACTCCGA CGCTGGAATG TTCCAGTGCG TGGGCACCAA TGCCGCTGGC 
AGTGTTCATG CTGCAGCTCG TCTGCGAGTT GTTCCGCAAG GAGTGAAAAT CAAAAAACGA 
AAATCCCAAG GCCACTCCAC CAAATCCCTT CAGACCTTTG ACAATCTGAC CAAGCGTCGC 
AAACCCTTCA ATTCGGGTGA GCAGCTGCGA ACCAAGTCCG GCGGGAGTTT TCCCTTTCCG 
GACGAGGACG ATGACCTAGA CGACAATGAC AGCGGTACGA CTCGACTAAG GATCCCATCC 
GGAAAGCTTC CCAAATTTGA GCAACCGCTC AACGACCGCA GATTCAATAT CCTAAACGCG 
GGAAGTGCTT TGCCGCTGGA CAACCAATCG AAGAGTTACG AGGACGATGA CGACGACTAT 
GACGAGGACG ATCCAGCTAA GGAGGCTCTC CTGGAGGCCT ACAAACAGGG CGAGGACGCA 
CATAAAATAC TGGACTCCTG GCAGCAGTCC AAGAGCAAGA AATCCCAACA GCAAAAACAA 
TCCCCTGCTT CCCCAGACTC CCCCGAACAG GATCCGAGTG TTCCGCATCC AGGTGGCAAG 
CCGTTGGACA GCGGACTCCA GGCCCGATTG CCCAGCCAGC CGCGCGATCT GGTGGCGCAG 
ATAGTGAAGT CCCGATTCGT GACCCTCAGC TGGGTGGAAC CACTCCAAAA TGCCGGCGAT 
GTAGTCTACT ACACGGTCTA CTACAAGATG AACAACAGCG AGAGGGAGCA AAAAATGGTT 
ACCAAGTCCC ATGACGATCA GCAGGTTAAT ATTCAATCGC TACTGCCCGG TAGAACCTAT 
CAGTTCCGAG TGGAGGCCAA TACCAATTTC GGCAGCGGTG CATCTTCTGC TCCGCTAGAA 
GTTAGTACCC AACCGGAGGT GAACATTGCA GGTCCGCCGC GCAACTTTGA AGGCTATGCA 
CGCAGTCACA AGGAGATCTA CGTCAAATGG GAAGAGCCCA CGGTAACCAA TGGAGAAATA 
CTCAAGTATC GAGTGTACTA TTCCGAGAAC GACAGTGGTG CCGATCTATA TCACGATAGT 
ACTGCTTTGG AAGCAGTTCT TACCGAGTTG CGTCCCCACA CGGATTACGT GATTTCCGTA 
GTGCCTTTTA ATCGAAACGG AATGGGTGAC TCCTCAGCGG AGATACGTGT TAAGACGTTT 
TCGTCCACGC CGTCGGAACC TCCTAATAAT GTTACTCTCG AGGTGACCAG CTCCAGCTCC 
ATTACCGTCC ACTGGGAGCC GCCTGCAGAG GAAGATCGCA ATGGCCAAAT TACGGGCTAC 
AAGATTCGCT ATCGCAAGTT CAAGGATGCA CCGCAGGTCA AGAGTACTCC TGCCAATATT 
CGTTACTTTG AGTTGAGCAA CTTGGACCGC AATGCCGAGT ATCAGGTGAA GATCGCTGCA 
ATGACAGTGA ACGGTTCCGG ACCCTTCACG GAATGGAATC GTGCTAACAC CTTGGAAAAC 
GATCTGGACG AGACCCAAGT TCCGGGCAAA CCAATTTGGA TTAGCATTCA TCCTGGAGCC 
AACAACATTG CCCTGCATTG GGGTCCCCCA CAGCATCCGG AAATCAAGAT ACGCAACTAC 
GTGCTGGGCT GGGGCCGTGG CATTCCCGAT GAGAACACCA TAGAGCTAAA GGAGACTGAG 
CGCTATCATA TACTCAAGAA TCTAGAATCG AATATGGATT ATGTGGTGTC GCTAAGGGCT 
AGAAATGTTA AAGGTGACGG TCCGCCTATA TATGACAACA TTAAGACACG CGACGAGGAG 
CCCGTGGACG CACCCACTCC ACTTGAGGTT CCGGTCGGTC TGCGAGCCAT TACCATGTCC 
AGCTCGTCGA TTGTGGTCTA CTGGATTGAC ACGATGCTGA ACAAAAATCA GCATGTAACA 
GATAATCGCC ACTATACGGT TAGTTATGGC ATCACGGGAT CCAATCGCTA TCGCTATCAC 
AACACCACGG ATCTAAACTG CATGATTAAT GATCTACGAC CCAATACGCA GTATGAGTTT 
GCAGTTAAAG TGGTCAAAGG ACGAAGGGAG TCCTCCTGGT CGATGTCTGT GTTAAATAGT 
ACATATCAGA ATGTTCCTGT CACCCCGCCT CGAGAGGTTA CTGTGCGCTT GGATGAGATG 
AATCCGCCCA CTGTGATCGT CCAGTGGATA CCTCCGAAAC ATACTCTTGG TCAGATTACC 
GGATACAATA TTTACTATAC CACCGACACC ACGAAAAGAG ATCGCGACTG GTCAGTTGAA 
GCATTTGCCG GCGAGGAAAC TATGCTCATG CTACCGAATC TGAAACCTTA CACAACGTAT 
TACTTTAAGG TTCAGGCTAG AACAACCAAG GGGGCTAATA ATGCTCCTTT CTCGGCTCTG 
GTATCGTACA CCACAAGTGC AGCCGTTACA ATGCAGGAGC CCGACACCAT AGCCAAGGGA 
ATCGACAACG AGAAGCTGTT GTATATAATT ATAGCAGCTA CCGCTGTAGT TCTACTTGTG 
GTGCTCTTGG GCGTCCTATT GCTCTGCCGA CGTAAGCCAC AATCTTCGCC AGAACACACT 
AAGAAGAGCT ATCAAAAGAA CAATGTTGGC GTGCCCAAGC CACCAGATCT ATGGATACAC 
CATGATCAAA TGGAGCTCAA AAACATAGAC AAGGGCCTGC ACACAGTGAC ACCCGTCTGC 
AGCGATGGAG CCTCGAGCAG TGGTGCCCTC ACCCTGCCAC GATCAGTGGT GCACAGTGAA 
TACGAGGTGG AGACGCCAGT GCCCGGTCAT GTGACCAATT CTCTGGACAA GCGGTCCTAT 
GTGCCAGGAT ATATGACCAC ATCAATGAAC GGAACGATGG AGCGACCTCA GTACCCGCGC 
ACCCAGTACA GCCACCAAAA TCGTTCTCAC ATGACCATGG AGGCTGGCCT CTCCCAGCAG 
AGTCTCACCC AACCGCAAAG CAACTCGATG GCTCAGACCC CAGAGCATCC TTATGGCGGC 
TACGATGCCA ACTTCTGTAA CGCGGGAAAT GCAGCTGCAG GCAATGGCTG TGTGTCCACC 
ATTGAAAGTT CTAAGCGTGG CCATCCTCTA AAGAGCTTTA GTGTGCCGGG GCCACCACCC 
ACTGGCGGAG CCACCCCAGT TACCAAGCAT ACTCCCGCCG TCACAATACG TCCACAAAAT 
CAATCGCCCT ACAAGAAACC ATCTTTCTCG GCTGCCACGC CCAATCGCCT GCAGGGCGGT 
GGCTCGGTAG TGCACTCAAC CGATGAGATT CAAAGACTTG CCCCCAGCAC ATCTACCGAG 
GAGCTAAACC AAGAAATGGC CAATCTGGAG GGCCTCATGA AGGATCTAAG CGCAATCACG 
GCCAACGAGT TCGAGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523716.2
CDS	675..5255
Translation	MAITTNRSSRTLWNWLLSSCLIFQLIGSSLASQALSFTLEPQDAVVPEGHSVLLQCAGTASIGRGGKSKSNLPSSVSIRWRGPDGQDLVIVGDTFRTQLKNGSLYISSVEENRGLTGAYQCLLTAEGVGSILSRPALVAIVRQPDLNQDFLETYLLPGQTAYFRCMLGEANWQEGVKHSVQWLKDDLPLPLDKLRMVVLPNGALEIDEVGPSDRGSYQCNVTSGSSSRLSSKTNLNIKKPSDPGAENSVAPSFLVGPSPKTVREGDTVTLDCVANGVPKPQIKWLRNGMDLDFNDLDSRFSIVGTGSLQISSAEDIDSGNYQCRASNTVDSLDAQATVQVQEPPKFIKAPKDTTAHEKDEPELKCDIWGKPKPVIRWLKNGDLITPNDYMQLVDGHNLKILGLLNSDAGMFQCVGTNAAGSVHAAARLRVVPQGVKIKKRKSQGHSTKSLQTFDNLTKRRKPFNSGEQLRTKSGGSFPFPDEDDDLDDNDSGTTRLRIPSGKLPKFEQPLNDRRFNILNAGSALPLDNQSKSYEDDDDDYDEDDPAKEALLEAYKQGEDAHKILDSWQQSKSKKSQQQKQSPASPDSPEQDPSVPHPGGKPLDSGLQARLPSQPRDLVAQIVKSRFVTLSWVEPLQNAGDVVYYTVYYKMNNSEREQKMVTKSHDDQQVNIQSLLPGRTYQFRVEANTNFGSGASSAPLEVSTQPEVNIAGPPRNFEGYARSHKEIYVKWEEPTVTNGEILKYRVYYSENDSGADLYHDSTALEAVLTELRPHTDYVISVVPFNRNGMGDSSAEIRVKTFSSTPSEPPNNVTLEVTSSSSITVHWEPPAEEDRNGQITGYKIRYRKFKDAPQVKSTPANIRYFELSNLDRNAEYQVKIAAMTVNGSGPFTEWNRANTLENDLDETQVPGKPIWISIHPGANNIALHWGPPQHPEIKIRNYVLGWGRGIPDENTIELKETERYHILKNLESNMDYVVSLRARNVKGDGPPIYDNIKTRDEEPVDAPTPLEVPVGLRAITMSSSSIVVYWIDTMLNKNQHVTDNRHYTVSYGITGSNRYRYHNTTDLNCMINDLRPNTQYEFAVKVVKGRRESSWSMSVLNSTYQNVPVTPPREVTVRLDEMNPPTVIVQWIPPKHTLGQITGYNIYYTTDTTKRDRDWSVEAFAGEETMLMLPNLKPYTTYYFKVQARTTKGANNAPFSALVSYTTSAAVTMQEPDTIAKGIDNEKLLYIIIAATAVVLLVVLLGVLLLCRRKPQSSPEHTKKSYQKNNVGVPKPPDLWIHHDQMELKNIDKGLHTVTPVCSDGASSSGALTLPRSVVHSEYEVETPVPGHVTNSLDKRSYVPGYMTTSMNGTMERPQYPRTQYSHQNRSHMTMEAGLSQQSLTQPQSNSMAQTPEHPYGGYDANFCNAGNAAAGNGCVSTIESSKRGHPLKSFSVPGPPPTGGATPVTKHTPAVTIRPQNQSPYKKPSFSAATPNRLQGGGSVVHSTDEIQRLAPSTSTEELNQEMANLEGLMKDLSAITANEFEC

Target ORF information:

RefSeq Version	NM_078992.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frazzled (fra), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078992.3

ORF Insert Sequence:

ATGGCCATCA CAACAAACAG AAGCAGCAGG ACTTTGTGGA ACTGGCTGCT CAGCAGTTGT 
CTCATATTCC AGCTGATTGG CAGCAGTTTG GCAAGTCAAG CTCTTTCGTT CACCTTGGAG 
CCCCAGGATG CCGTGGTGCC CGAGGGCCAC TCGGTGCTGC TCCAGTGTGC AGGCACTGCA 
TCCATTGGAC GTGGAGGCAA GAGCAAGTCC AACTTACCCA GCTCAGTGAG CATCCGGTGG 
CGAGGACCTG ATGGGCAGGA CCTGGTCATC GTGGGCGATA CGTTCCGCAC TCAGCTGAAG 
AATGGATCAC TGTACATTAG CTCCGTTGAG GAGAACCGTG GACTGACGGG AGCCTACCAA 
TGCCTGTTGA CTGCCGAGGG CGTGGGCAGC ATACTCAGTC GACCCGCACT GGTGGCCATT 
GTCCGACAGC CAGATCTGAA TCAGGACTTC CTGGAAACCT ACCTGCTGCC AGGTCAGACG 
GCCTACTTCC GTTGCATGCT GGGCGAAGCC AACTGGCAGG AGGGCGTTAA GCATTCGGTA 
CAGTGGCTCA AGGATGACCT GCCACTGCCC CTGGACAAAC TGCGCATGGT GGTGCTGCCC 
AACGGAGCGC TGGAGATCGA CGAAGTGGGT CCATCGGATC GCGGATCCTA TCAGTGCAAC 
GTGACTTCGG GCAGTTCCTC CCGGCTAAGC AGCAAAACGA ACTTGAACAT TAAGAAACCC 
AGTGATCCGG GTGCCGAGAA CTCAGTGGCA CCATCGTTCC TTGTGGGACC TTCGCCCAAA 
ACCGTGAGAG AGGGCGACAC CGTCACCCTG GACTGTGTGG CCAACGGAGT GCCAAAGCCG 
CAGATTAAGT GGCTGCGAAA TGGCATGGAT CTGGATTTTA ACGACCTGGA CTCACGCTTT 
TCCATCGTTG GAACGGGTTC ACTGCAAATC TCCAGTGCCG AGGACATTGA TTCAGGGAAC 
TACCAGTGCC GGGCCAGCAA TACGGTGGAT TCTCTTGATG CTCAGGCCAC GGTGCAGGTA 
CAGGAACCAC CTAAGTTCAT TAAGGCGCCC AAGGATACCA CTGCCCACGA GAAGGACGAA 
CCGGAATTGA AGTGCGATAT CTGGGGAAAG CCAAAGCCCG TAATAAGGTG GCTTAAGAAC 
GGAGATCTCA TAACGCCCAA TGACTACATG CAACTGGTGG ATGGTCACAA TCTGAAGATC 
CTCGGACTTC TCAACTCCGA CGCTGGAATG TTCCAGTGCG TGGGCACCAA TGCCGCTGGC 
AGTGTTCATG CTGCAGCTCG TCTGCGAGTT GTTCCGCAAG GAGTGAAAAT CAAAAAACGA 
AAATCCCAAG GCCACTCCAC CAAATCCCTT CAGACCTTTG ACAATCTGAC CAAGCGTCGC 
AAACCCTTCA ATTCGGGTGA GCAGCTGCGA ACCAAGTCCG GCGGGAGTTT TCCCTTTCCG 
GACGAGGACG ATGACCTAGA CGACAATGAC AGCGGTACGA CTCGACTAAG GATCCCATCC 
GGAAAGCTTC CCAAATTTGA GCAACCGCTC AACGACCGCA GATTCAATAT CCTAAACGCG 
GGAAGTGCTT TGCCGCTGGA CAACCAATCG AAGAGTTACG AGGACGATGA CGACGACTAT 
GACGAGGACG ATCCAGCTAA GGAGGCTCTC CTGGAGGCCT ACAAACAGGG CGAGGACGCA 
CATAAAATAC TGGACTCCTG GCAGCAGTCC AAGAGCAAGA AATCCCAACA GCAAAAACAA 
TCCCCTGCTT CCCCAGACTC CCCCGAACAG GATCCGAGTG TTCCGCATCC AGGTGGCAAG 
CCGTTGGACA GCGGACTCCA GGCCCGATTG CCCAGCCAGC CGCGCGATCT GGTGGCGCAG 
ATAGTGAAGT CCCGATTCGT GACCCTCAGC TGGGTGGAAC CACTCCAAAA TGCCGGCGAT 
GTAGTCTACT ACACGGTCTA CTACAAGATG AACAACAGCG AGAGGGAGCA AAAAATGGTT 
ACCAAGTCCC ATGACGATCA GCAGGTTAAT ATTCAATCGC TACTGCCCGG TAGAACCTAT 
CAGTTCCGAG TGGAGGCCAA TACCAATTTC GGCAGCGGTG CATCTTCTGC TCCGCTAGAA 
GTTAGTACCC AACCGGAGGT GAACATTGCA GGTCCGCCGC GCAACTTTGA AGGCTATGCA 
CGCAGTCACA AGGAGATCTA CGTCAAATGG GAAGAGCCCA CGGTAACCAA TGGAGAAATA 
CTCAAGTATC GAGTGTACTA TTCCGAGAAC GACAGTGGTG CCGATCTATA TCACGATAGT 
ACTGCTTTGG AAGCAGTTCT TACCGAGTTG CGTCCCCACA CGGATTACGT GATTTCCGTA 
GTGCCTTTTA ATCGAAACGG AATGGGTGAC TCCTCAGCGG AGATACGTGT TAAGACGTTT 
TCGTCCACGC CGTCGGAACC TCCTAATAAT GTTACTCTCG AGGTGACCAG CTCCAGCTCC 
ATTACCGTCC ACTGGGAGCC GCCTGCAGAG GAAGATCGCA ATGGCCAAAT TACGGGCTAC 
AAGATTCGCT ATCGCAAGTT CAAGGATGCA CCGCAGGTCA AGAGTACTCC TGCCAATATT 
CGTTACTTTG AGTTGAGCAA CTTGGACCGC AATGCCGAGT ATCAGGTGAA GATCGCTGCA 
ATGACAGTGA ACGGTTCCGG ACCCTTCACG GAATGGAATC GTGCTAACAC CTTGGAAAAC 
GATCTGGACG AGACCCAAGT TCCGGGCAAA CCAATTTGGA TTAGCATTCA TCCTGGAGCC 
AACAACATTG CCCTGCATTG GGGTCCCCCA CAGCATCCGG AAATCAAGAT ACGCAACTAC 
GTGCTGGGCT GGGGCCGTGG CATTCCCGAT GAGAACACCA TAGAGCTAAA GGAGACTGAG 
CGCTATCATA TACTCAAGAA TCTAGAATCG AATATGGATT ATGTGGTGTC GCTAAGGGCT 
AGAAATGTTA AAGGTGACGG TCCGCCTATA TATGACAACA TTAAGACACG CGACGAGGAG 
CCCGTGGACG CACCCACTCC ACTTGAGGTT CCGGTCGGTC TGCGAGCCAT TACCATGTCC 
AGCTCGTCGA TTGTGGTCTA CTGGATTGAC ACGATGCTGA ACAAAAATCA GCATGTAACA 
GATAATCGCC ACTATACGGT TAGTTATGGC ATCACGGGAT CCAATCGCTA TCGCTATCAC 
AACACCACGG ATCTAAACTG CATGATTAAT GATCTACGAC CCAATACGCA GTATGAGTTT 
GCAGTTAAAG TGGTCAAAGG ACGAAGGGAG TCCTCCTGGT CGATGTCTGT GTTAAATAGT 
ACATATCAGA ATGTTCCTGT CACCCCGCCT CGAGAGGTTA CTGTGCGCTT GGATGAGATG 
AATCCGCCCA CTGTGATCGT CCAGTGGATA CCTCCGAAAC ATACTCTTGG TCAGATTACC 
GGATACAATA TTTACTATAC CACCGACACC ACGAAAAGAG ATCGCGACTG GTCAGTTGAA 
GCATTTGCCG GCGAGGAAAC TATGCTCATG CTACCGAATC TGAAACCTTA CACAACGTAT 
TACTTTAAGG TTCAGGCTAG AACAACCAAG GGGGCTAATA ATGCTCCTTT CTCGGCTCTG 
GTATCGTACA CCACAAGTGC AGCCGTTACA ATGCAGGAGC CCGACACCAT AGCCAAGGGA 
ATCGACAACG AGAAGCTGTT GTATATAATT ATAGCAGCTA CCGCTGTAGT TCTACTTGTG 
GTGCTCTTGG GCGTCCTATT GCTCTGCCGA CGTAAGCCAC AATCTTCGCC AGAACACACT 
AAGAAGAGCT ATCAAAAGAA CAATGTTGGC GTGCCCAAGC CACCAGATCT ATGGATACAC 
CATGATCAAA TGGAGCTCAA AAACATAGAC AAGGGCCTGC ACACAGTGAC ACCCGTCTGC 
AGCGATGGAG CCTCGAGCAG TGGTGCCCTC ACCCTGCCAC GATCAGTGGT GCACAGTGAA 
TACGAGGTGG AGACGCCAGT GCCCGGTCAT GTGACCAATT CTCTGGACAA GCGGTCCTAT 
GTGCCAGGAT ATATGACCAC ATCAATGAAC GGAACGATGG AGCGACCTCA GTACCCGCGC 
ACCCAGTACA GCCACCAAAA TCGTTCTCAC ATGACCATGG AGGCTGGCCT CTCCCAGCAG 
AGTCTCACCC AACCGCAAAG CAACTCGATG GCTCAGACCC CAGAGCATCC TTATGGCGGC 
TACGATGCCA ACTTCTGTAA CGCGGGAAAT GCAGCTGCAG GCAATGGCTG TGTGTCCACC 
ATTGAAAGTT CTAAGCGTGG CCATCCTCTA AAGAGCTTTA GTGTGCCGGG GCCACCACCC 
ACTGGCGGAG CCACCCCAGT TACCAAGCAT ACTCCCGCCG TCACAATACG TCCACAAAAT 
CAATCGCCCT ACAAGAAACC ATCTTTCTCG GCTGCCACGC CCAATCGCCT GCAGGGCGGT 
GGCTCGGTAG TGCACTCAAC CGATGAGATT CAAAGACTTG CCCCCAGCAC ATCTACCGAG 
GAGCTAAACC AAGAAATGGC CAATCTGGAG GGCCTCATGA AGGATCTAAG CGCAATCACG 
GCCAACGAGT TCGAGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fra ( NM_165913.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725185.1 Drosophila melanogaster frazzled (fra), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09772
Clone ID Related Accession (Same CDS sequence)	NM_165913.2
Accession Version	NM_165913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frazzled, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165913.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATCA CAACAAACAG AAGCAGCAGG ACTTTGTGGA ACTGGCTGCT CAGCAGTTGT 
CTCATATTCC AGCTGATTGG CAGCAGTTTG GCAAGTCAAG CTCTTTCGTT CACCTTGGAG 
CCCCAGGATG CCGTGGTGCC CGAGGGCCAC TCGGTGCTGC TCCAGTGTGC AGGCACTGCA 
TCCATTGGAC GTGGAGGCAA GAGCAAGTCC AACTTACCCA GCTCAGTGAG CATCCGGTGG 
CGAGGACCTG ATGGGCAGGA CCTGGTCATC GTGGGCGATA CGTTCCGCAC TCAGCTGAAG 
AATGGATCAC TGTACATTAG CTCCGTTGAG GAGAACCGTG GACTGACGGG AGCCTACCAA 
TGCCTGTTGA CTGCCGAGGG CGTGGGCAGC ATACTCAGTC GACCCGCACT GGTGGCCATT 
GTCCGACAGC CAGATCTGAA TCAGGACTTC CTGGAAACCT ACCTGCTGCC AGGTCAGACG 
GCCTACTTCC GTTGCATGCT GGGCGAAGCC AACTGGCAGG AGGGCGTTAA GCATTCGGTA 
CAGTGGCTCA AGGATGACCT GCCACTGCCC CTGGACAAAC TGCGCATGGT GGTGCTGCCC 
AACGGAGCGC TGGAGATCGA CGAAGTGGGT CCATCGGATC GCGGATCCTA TCAGTGCAAC 
GTGACTTCGG GCAGTTCCTC CCGGCTAAGC AGCAAAACGA ACTTGAACAT TAAGAAACCC 
AGTGATCCGG GTGCCGAGAA CTCAGTGGCA CCATCGTTCC TTGTGGGACC TTCGCCCAAA 
ACCGTGAGAG AGGGCGACAC CGTCACCCTG GACTGTGTGG CCAACGGAGT GCCAAAGCCG 
CAGATTAAGT GGCTGCGAAA TGGCATGGAT CTGGATTTTA ACGACCTGGA CTCACGCTTT 
TCCATCGTTG GAACGGGTTC ACTGCAAATC TCCAGTGCCG AGGACATTGA TTCAGGGAAC 
TACCAGTGCC GGGCCAGCAA TACGGTGGAT TCTCTTGATG CTCAGGCCAC GGTGCAGGTA 
CAGGAACCAC CTAAGTTCAT TAAGGCGCCC AAGGATACCA CTGCCCACGA GAAGGACGAA 
CCGGAATTGA AGTGCGATAT CTGGGGAAAG CCAAAGCCCG TAATAAGGTG GCTTAAGAAC 
GGAGATCTCA TAACGCCCAA TGACTACATG CAACTGGTGG ATGGTCACAA TCTGAAGATC 
CTCGGACTTC TCAACTCCGA CGCTGGAATG TTCCAGTGCG TGGGCACCAA TGCCGCTGGC 
AGTGTTCATG CTGCAGCTCG TCTGCGAGTT GTTCCGCAAG GAGACTCCCC CGAACAGGAT 
CCGAGTGTTC CGCATCCAGG TGGCAAGCCG TTGGACAGCG GACTCCAGGC CCGATTGCCC 
AGCCAGCCGC GCGATCTGGT GGCGCAGATA GTGAAGTCCC GATTCGTGAC CCTCAGCTGG 
GTGGAACCAC TCCAAAATGC CGGCGATGTA GTCTACTACA CGGTCTACTA CAAGATGAAC 
AACAGCGAGA GGGAGCAAAA AATGGTTACC AAGTCCCATG ACGATCAGCA GGTTAATATT 
CAATCGCTAC TGCCCGGTAG AACCTATCAG TTCCGAGTGG AGGCCAATAC CAATTTCGGC 
AGCGGTGCAT CTTCTGCTCC GCTAGAAGTT AGTACCCAAC CGGAGGTGAA CATTGCAGGT 
CCGCCGCGCA ACTTTGAAGG CTATGCACGC AGTCACAAGG AGATCTACGT CAAATGGGAA 
GAGCCCACGG TAACCAATGG AGAAATACTC AAGTATCGAG TGTACTATTC CGAGAACGAC 
AGTGGTGCCG ATCTATATCA CGATAGTACT GCTTTGGAAG CAGTTCTTAC CGAGTTGCGT 
CCCCACACGG ATTACGTGAT TTCCGTAGTG CCTTTTAATC GAAACGGAAT GGGTGACTCC 
TCAGCGGAGA TACGTGTTAA GACGTTTTCG TCCACGCCGT CGGAACCTCC TAATAATGTT 
ACTCTCGAGG TGACCAGCTC CAGCTCCATT ACCGTCCACT GGGAGCCGCC TGCAGAGGAA 
GATCGCAATG GCCAAATTAC GGGCTACAAG ATTCGCTATC GCAAGTTCAA GGATGCACCG 
CAGGTCAAGA GTACTCCTGC CAATATTCGT TACTTTGAGT TGAGCAACTT GGACCGCAAT 
GCCGAGTATC AGGTGAAGAT CGCTGCAATG ACAGTGAACG GTTCCGGACC CTTCACGGAA 
TGGAATCGTG CTAACACCTT GGAAAACGAT CTGGACGAGA CCCAAGTTCC GGGCAAACCA 
ATTTGGATTA GCATTCATCC TGGAGCCAAC AACATTGCCC TGCATTGGGG TCCCCCACAG 
CATCCGGAAA TCAAGATACG CAACTACGTG CTGGGCTGGG GCCGTGGCAT TCCCGATGAG 
AACACCATAG AGCTAAAGGA GACTGAGCGC TATCATATAC TCAAGAATCT AGAATCGAAT 
ATGGATTATG TGGTGTCGCT AAGGGCTAGA AATGTTAAAG GTGACGGTCC GCCTATATAT 
GACAACATTA AGACACGCGA CGAGGAGCCC GTGGACGCAC CCACTCCACT TGAGGTTCCG 
GTCGGTCTGC GAGCCATTAC CATGTCCAGC TCGTCGATTG TGGTCTACTG GATTGACACG 
ATGCTGAACA AAAATCAGCA TGTAACAGAT AATCGCCACT ATACGGTTAG TTATGGCATC 
ACGGGATCCA ATCGCTATCG CTATCACAAC ACCACGGATC TAAACTGCAT GATTAATGAT 
CTACGACCCA ATACGCAGTA TGAGTTTGCA GTTAAAGTGG TCAAAGGACG AAGGGAGTCC 
TCCTGGTCGA TGTCTGTGTT AAATAGTACA TATCAGAATG TTCCTGTCAC CCCGCCTCGA 
GAGGTTACTG TGCGCTTGGA TGAGATGAAT CCGCCCACTG TGATCGTCCA GTGGATACCT 
CCGAAACATA CTCTTGGTCA GATTACCGGA TACAATATTT ACTATACCAC CGACACCACG 
AAAAGAGATC GCGACTGGTC AGTTGAAGCA TTTGCCGGCG AGGAAACTAT GCTCATGCTA 
CCGAATCTGA AACCTTACAC AACGTATTAC TTTAAGGTTC AGGCTAGAAC AACCAAGGGG 
GCTAATAATG CTCCTTTCTC GGCTCTGGTA TCGTACACCA CAAGTGCAGC CGTTACAATG 
CAGGAGCCCG ACACCATAGC CAAGGGAATC GACAACGAGA AGCTGTTGTA TATAATTATA 
GCAGCTACCG CTGTAGTTCT ACTTGTGGTG CTCTTGGGCG TCCTATTGCT CTGCCGACGT 
AAGCCACAAT CTTCGCCAGA ACACACTAAG AAGAGCTATC AAAAGAACAA TGTTGGCGTG 
CCCAAGCCAC CAGATCTATG GATACACCAT GATCAAATGG AGCTCAAAAA CATAGACAAG 
GGCCTGCACA CAGTGACACC CGTCTGCAGC GATGGAGCCT CGAGCAGTGG TGCCCTCACC 
CTGCCACGAT CAGTGGTGCA CAGTGAATAC GAGGTGGAGA CGCCAGTGCC CGGTCATGTG 
ACCAATTCTC TGGACAAGCG GTCCTATGTG CCAGGATATA TGACCACATC AATGAACGGA 
ACGATGGAGC GACCTCAGTA CCCGCGCACC CAGTACAGCC ACCAAAATCG TTCTCACATG 
ACCATGGAGG CTGGCCTCTC CCAGCAGAGT CTCACCCAAC CGCAAAGCAA CTCGATGGCT 
CAGACCCCAG AGCATCCTTA TGGCGGCTAC GATGCCAACT TCTGTAACGC GGGAAATGCA 
GCTGCAGGCA ATGGCTGTGT GTCCACCATT GAAAGTTCTA AGCGTGGCCA TCCTCTAAAG 
AGCTTTAGTG TGCCGGGGCC ACCACCCACT GGCGGAGCCA CCCCAGTTAC CAAGCATACT 
CCCGCCGTCA CAATACGTCC ACAAAATCAA TCGCCCTACA AGAAACCATC TTTCTCGGCT 
GCCACGCCCA ATCGCCTGCA GGGCGGTGGC TCGGTAGTGC ACTCAACCGA TGAGATTCAA 
AGACTTGCCC CCAGCACATC TACCGAGGAG CTAAACCAAG AAATGGCCAA TCTGGAGGGC 
CTCATGAAGG ATCTAAGCGC AATCACGGCC AACGAGTTCG AGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725185.1
CDS	675..4802
Translation	MAITTNRSSRTLWNWLLSSCLIFQLIGSSLASQALSFTLEPQDAVVPEGHSVLLQCAGTASIGRGGKSKSNLPSSVSIRWRGPDGQDLVIVGDTFRTQLKNGSLYISSVEENRGLTGAYQCLLTAEGVGSILSRPALVAIVRQPDLNQDFLETYLLPGQTAYFRCMLGEANWQEGVKHSVQWLKDDLPLPLDKLRMVVLPNGALEIDEVGPSDRGSYQCNVTSGSSSRLSSKTNLNIKKPSDPGAENSVAPSFLVGPSPKTVREGDTVTLDCVANGVPKPQIKWLRNGMDLDFNDLDSRFSIVGTGSLQISSAEDIDSGNYQCRASNTVDSLDAQATVQVQEPPKFIKAPKDTTAHEKDEPELKCDIWGKPKPVIRWLKNGDLITPNDYMQLVDGHNLKILGLLNSDAGMFQCVGTNAAGSVHAAARLRVVPQGDSPEQDPSVPHPGGKPLDSGLQARLPSQPRDLVAQIVKSRFVTLSWVEPLQNAGDVVYYTVYYKMNNSEREQKMVTKSHDDQQVNIQSLLPGRTYQFRVEANTNFGSGASSAPLEVSTQPEVNIAGPPRNFEGYARSHKEIYVKWEEPTVTNGEILKYRVYYSENDSGADLYHDSTALEAVLTELRPHTDYVISVVPFNRNGMGDSSAEIRVKTFSSTPSEPPNNVTLEVTSSSSITVHWEPPAEEDRNGQITGYKIRYRKFKDAPQVKSTPANIRYFELSNLDRNAEYQVKIAAMTVNGSGPFTEWNRANTLENDLDETQVPGKPIWISIHPGANNIALHWGPPQHPEIKIRNYVLGWGRGIPDENTIELKETERYHILKNLESNMDYVVSLRARNVKGDGPPIYDNIKTRDEEPVDAPTPLEVPVGLRAITMSSSSIVVYWIDTMLNKNQHVTDNRHYTVSYGITGSNRYRYHNTTDLNCMINDLRPNTQYEFAVKVVKGRRESSWSMSVLNSTYQNVPVTPPREVTVRLDEMNPPTVIVQWIPPKHTLGQITGYNIYYTTDTTKRDRDWSVEAFAGEETMLMLPNLKPYTTYYFKVQARTTKGANNAPFSALVSYTTSAAVTMQEPDTIAKGIDNEKLLYIIIAATAVVLLVVLLGVLLLCRRKPQSSPEHTKKSYQKNNVGVPKPPDLWIHHDQMELKNIDKGLHTVTPVCSDGASSSGALTLPRSVVHSEYEVETPVPGHVTNSLDKRSYVPGYMTTSMNGTMERPQYPRTQYSHQNRSHMTMEAGLSQQSLTQPQSNSMAQTPEHPYGGYDANFCNAGNAAAGNGCVSTIESSKRGHPLKSFSVPGPPPTGGATPVTKHTPAVTIRPQNQSPYKKPSFSAATPNRLQGGGSVVHSTDEIQRLAPSTSTEELNQEMANLEGLMKDLSAITANEFEC

Target ORF information:

RefSeq Version	NM_165913.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frazzled (fra), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165913.2

ORF Insert Sequence:

ATGGCCATCA CAACAAACAG AAGCAGCAGG ACTTTGTGGA ACTGGCTGCT CAGCAGTTGT 
CTCATATTCC AGCTGATTGG CAGCAGTTTG GCAAGTCAAG CTCTTTCGTT CACCTTGGAG 
CCCCAGGATG CCGTGGTGCC CGAGGGCCAC TCGGTGCTGC TCCAGTGTGC AGGCACTGCA 
TCCATTGGAC GTGGAGGCAA GAGCAAGTCC AACTTACCCA GCTCAGTGAG CATCCGGTGG 
CGAGGACCTG ATGGGCAGGA CCTGGTCATC GTGGGCGATA CGTTCCGCAC TCAGCTGAAG 
AATGGATCAC TGTACATTAG CTCCGTTGAG GAGAACCGTG GACTGACGGG AGCCTACCAA 
TGCCTGTTGA CTGCCGAGGG CGTGGGCAGC ATACTCAGTC GACCCGCACT GGTGGCCATT 
GTCCGACAGC CAGATCTGAA TCAGGACTTC CTGGAAACCT ACCTGCTGCC AGGTCAGACG 
GCCTACTTCC GTTGCATGCT GGGCGAAGCC AACTGGCAGG AGGGCGTTAA GCATTCGGTA 
CAGTGGCTCA AGGATGACCT GCCACTGCCC CTGGACAAAC TGCGCATGGT GGTGCTGCCC 
AACGGAGCGC TGGAGATCGA CGAAGTGGGT CCATCGGATC GCGGATCCTA TCAGTGCAAC 
GTGACTTCGG GCAGTTCCTC CCGGCTAAGC AGCAAAACGA ACTTGAACAT TAAGAAACCC 
AGTGATCCGG GTGCCGAGAA CTCAGTGGCA CCATCGTTCC TTGTGGGACC TTCGCCCAAA 
ACCGTGAGAG AGGGCGACAC CGTCACCCTG GACTGTGTGG CCAACGGAGT GCCAAAGCCG 
CAGATTAAGT GGCTGCGAAA TGGCATGGAT CTGGATTTTA ACGACCTGGA CTCACGCTTT 
TCCATCGTTG GAACGGGTTC ACTGCAAATC TCCAGTGCCG AGGACATTGA TTCAGGGAAC 
TACCAGTGCC GGGCCAGCAA TACGGTGGAT TCTCTTGATG CTCAGGCCAC GGTGCAGGTA 
CAGGAACCAC CTAAGTTCAT TAAGGCGCCC AAGGATACCA CTGCCCACGA GAAGGACGAA 
CCGGAATTGA AGTGCGATAT CTGGGGAAAG CCAAAGCCCG TAATAAGGTG GCTTAAGAAC 
GGAGATCTCA TAACGCCCAA TGACTACATG CAACTGGTGG ATGGTCACAA TCTGAAGATC 
CTCGGACTTC TCAACTCCGA CGCTGGAATG TTCCAGTGCG TGGGCACCAA TGCCGCTGGC 
AGTGTTCATG CTGCAGCTCG TCTGCGAGTT GTTCCGCAAG GAGACTCCCC CGAACAGGAT 
CCGAGTGTTC CGCATCCAGG TGGCAAGCCG TTGGACAGCG GACTCCAGGC CCGATTGCCC 
AGCCAGCCGC GCGATCTGGT GGCGCAGATA GTGAAGTCCC GATTCGTGAC CCTCAGCTGG 
GTGGAACCAC TCCAAAATGC CGGCGATGTA GTCTACTACA CGGTCTACTA CAAGATGAAC 
AACAGCGAGA GGGAGCAAAA AATGGTTACC AAGTCCCATG ACGATCAGCA GGTTAATATT 
CAATCGCTAC TGCCCGGTAG AACCTATCAG TTCCGAGTGG AGGCCAATAC CAATTTCGGC 
AGCGGTGCAT CTTCTGCTCC GCTAGAAGTT AGTACCCAAC CGGAGGTGAA CATTGCAGGT 
CCGCCGCGCA ACTTTGAAGG CTATGCACGC AGTCACAAGG AGATCTACGT CAAATGGGAA 
GAGCCCACGG TAACCAATGG AGAAATACTC AAGTATCGAG TGTACTATTC CGAGAACGAC 
AGTGGTGCCG ATCTATATCA CGATAGTACT GCTTTGGAAG CAGTTCTTAC CGAGTTGCGT 
CCCCACACGG ATTACGTGAT TTCCGTAGTG CCTTTTAATC GAAACGGAAT GGGTGACTCC 
TCAGCGGAGA TACGTGTTAA GACGTTTTCG TCCACGCCGT CGGAACCTCC TAATAATGTT 
ACTCTCGAGG TGACCAGCTC CAGCTCCATT ACCGTCCACT GGGAGCCGCC TGCAGAGGAA 
GATCGCAATG GCCAAATTAC GGGCTACAAG ATTCGCTATC GCAAGTTCAA GGATGCACCG 
CAGGTCAAGA GTACTCCTGC CAATATTCGT TACTTTGAGT TGAGCAACTT GGACCGCAAT 
GCCGAGTATC AGGTGAAGAT CGCTGCAATG ACAGTGAACG GTTCCGGACC CTTCACGGAA 
TGGAATCGTG CTAACACCTT GGAAAACGAT CTGGACGAGA CCCAAGTTCC GGGCAAACCA 
ATTTGGATTA GCATTCATCC TGGAGCCAAC AACATTGCCC TGCATTGGGG TCCCCCACAG 
CATCCGGAAA TCAAGATACG CAACTACGTG CTGGGCTGGG GCCGTGGCAT TCCCGATGAG 
AACACCATAG AGCTAAAGGA GACTGAGCGC TATCATATAC TCAAGAATCT AGAATCGAAT 
ATGGATTATG TGGTGTCGCT AAGGGCTAGA AATGTTAAAG GTGACGGTCC GCCTATATAT 
GACAACATTA AGACACGCGA CGAGGAGCCC GTGGACGCAC CCACTCCACT TGAGGTTCCG 
GTCGGTCTGC GAGCCATTAC CATGTCCAGC TCGTCGATTG TGGTCTACTG GATTGACACG 
ATGCTGAACA AAAATCAGCA TGTAACAGAT AATCGCCACT ATACGGTTAG TTATGGCATC 
ACGGGATCCA ATCGCTATCG CTATCACAAC ACCACGGATC TAAACTGCAT GATTAATGAT 
CTACGACCCA ATACGCAGTA TGAGTTTGCA GTTAAAGTGG TCAAAGGACG AAGGGAGTCC 
TCCTGGTCGA TGTCTGTGTT AAATAGTACA TATCAGAATG TTCCTGTCAC CCCGCCTCGA 
GAGGTTACTG TGCGCTTGGA TGAGATGAAT CCGCCCACTG TGATCGTCCA GTGGATACCT 
CCGAAACATA CTCTTGGTCA GATTACCGGA TACAATATTT ACTATACCAC CGACACCACG 
AAAAGAGATC GCGACTGGTC AGTTGAAGCA TTTGCCGGCG AGGAAACTAT GCTCATGCTA 
CCGAATCTGA AACCTTACAC AACGTATTAC TTTAAGGTTC AGGCTAGAAC AACCAAGGGG 
GCTAATAATG CTCCTTTCTC GGCTCTGGTA TCGTACACCA CAAGTGCAGC CGTTACAATG 
CAGGAGCCCG ACACCATAGC CAAGGGAATC GACAACGAGA AGCTGTTGTA TATAATTATA 
GCAGCTACCG CTGTAGTTCT ACTTGTGGTG CTCTTGGGCG TCCTATTGCT CTGCCGACGT 
AAGCCACAAT CTTCGCCAGA ACACACTAAG AAGAGCTATC AAAAGAACAA TGTTGGCGTG 
CCCAAGCCAC CAGATCTATG GATACACCAT GATCAAATGG AGCTCAAAAA CATAGACAAG 
GGCCTGCACA CAGTGACACC CGTCTGCAGC GATGGAGCCT CGAGCAGTGG TGCCCTCACC 
CTGCCACGAT CAGTGGTGCA CAGTGAATAC GAGGTGGAGA CGCCAGTGCC CGGTCATGTG 
ACCAATTCTC TGGACAAGCG GTCCTATGTG CCAGGATATA TGACCACATC AATGAACGGA 
ACGATGGAGC GACCTCAGTA CCCGCGCACC CAGTACAGCC ACCAAAATCG TTCTCACATG 
ACCATGGAGG CTGGCCTCTC CCAGCAGAGT CTCACCCAAC CGCAAAGCAA CTCGATGGCT 
CAGACCCCAG AGCATCCTTA TGGCGGCTAC GATGCCAACT TCTGTAACGC GGGAAATGCA 
GCTGCAGGCA ATGGCTGTGT GTCCACCATT GAAAGTTCTA AGCGTGGCCA TCCTCTAAAG 
AGCTTTAGTG TGCCGGGGCC ACCACCCACT GGCGGAGCCA CCCCAGTTAC CAAGCATACT 
CCCGCCGTCA CAATACGTCC ACAAAATCAA TCGCCCTACA AGAAACCATC TTTCTCGGCT 
GCCACGCCCA ATCGCCTGCA GGGCGGTGGC TCGGTAGTGC ACTCAACCGA TGAGATTCAA 
AGACTTGCCC CCAGCACATC TACCGAGGAG CTAAACCAAG AAATGGCCAA TCTGGAGGGC 
CTCATGAAGG ATCTAAGCGC AATCACGGCC AACGAGTTCG AGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fra ( NM_001299424.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286353.1 Drosophila melanogaster frazzled (fra), transcript variant C, mRNA.

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CloneID	OFa21786
Clone ID Related Accession (Same CDS sequence)	NM_001299424.1
Accession Version	NM_001299424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frazzled, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATCA CAACAAACAG AAGCAGCAGG ACTTTGTGGA ACTGGCTGCT CAGCAGTTGT 
CTCATATTCC AGCTGATTGG CAGCAGTTTG GCAAGTCAAG CTCTTTCGTT CACCTTGGAG 
CCCCAGGATG CCGTGGTGCC CGAGGGCCAC TCGGTGCTGC TCCAGTGTGC AGGCACTGCA 
TCCATTGGAC GTGGAGGCAA GAGCAAGTCC AACTTACCCA GCTCAGTGAG CATCCGGTGG 
CGAGGACCTG ATGGGCAGGA CCTGGTCATC GTGGGCGATA CGTTCCGCAC TCAGCTGAAG 
AATGGATCAC TGTACATTAG CTCCGTTGAG GAGAACCGTG GACTGACGGG AGCCTACCAA 
TGCCTGTTGA CTGCCGAGGG CGTGGGCAGC ATACTCAGTC GACCCGCACT GGTGGCCATT 
GTCCGACAGC CAGATCTGAA TCAGGACTTC CTGGAAACCT ACCTGCTGCC AGGTCAGACG 
GCCTACTTCC GTTGCATGCT GGGCGAAGCC AACTGGCAGG AGGGCGTTAA GCATTCGGTA 
CAGTGGCTCA AGGATGACCT GCCACTGCCC CTGGACAAAC TGCGCATGGT GGTGCTGCCC 
AACGGAGCGC TGGAGATCGA CGAAGTGGGT CCATCGGATC GCGGATCCTA TCAGTGCAAC 
GTGACTTCGG GCAGTTCCTC CCGGCTAAGC AGCAAAACGA ACTTGAACAT TAAGAAACCC 
AGTGATCCGG GTGCCGAGAA CTCAGTGGCA CCATCGTTCC TTGTGGGACC TTCGCCCAAA 
ACCGTGAGAG AGGGCGACAC CGTCACCCTG GACTGTGTGG CCAACGGAGT GCCAAAGCCG 
CAGATTAAGT GGCTGCGAAA TGGCATGGAT CTGGATTTTA ACGACCTGGA CTCACGCTTT 
TCCATCGTTG GAACGGGTTC ACTGCAAATC TCCAGTGCCG AGGACATTGA TTCAGGGAAC 
TACCAGTGCC GGGCCAGCAA TACGGTGGAT TCTCTTGATG CTCAGGCCAC GGTGCAGGTA 
CAGGAACCAC CTAAGTTCAT TAAGGCGCCC AAGGATACCA CTGCCCACGA GAAGGACGAA 
CCGGAATTGA AGTGCGATAT CTGGGGAAAG CCAAAGCCCG TAATAAGGTG GCTTAAGAAC 
GGAGATCTCA TAACGCCCAA TGACTACATG CAACTGGTGG ATGGTCACAA TCTGAAGATC 
CTCGGACTTC TCAACTCCGA CGCTGGAATG TTCCAGTGCG TGGGCACCAA TGCCGCTGGC 
AGTGTTCATG CTGCAGCTCG TCTGCGAGTT GTTCCGCAAG GAGGCCACTC CACCAAATCC 
CTTCAGACCT TTGACAATCT GACCAAGCGT CGCAAACCCT TCAATTCGGG TGAGCAGCTG 
CGAACCAAGT CCGGCGGGAG TTTTCCCTTT CCGGACGAGG ACGATGACCT AGACGACAAT 
GACAGCGGTA CGACTCGACT AAGGATCCCA TCCGGAAAGC TTCCCAAATT TGAGCAACCG 
CTCAACGACC GCAGATTCAA TATCCTAAAC GCGGGAAGTG CTTTGCCGCT GGACAACCAA 
TCGAAGAGTT ACGAGGACGA TGACGACGAC TATGACGAGG ACGATCCAGC TAAGGAGGCT 
CTCCTGGAGG CCTACAAACA GGGCGAGGAC GCACATAAAA TACTGGACTC CTGGCAGCAG 
TCCAAGAGCA AGAAATCCCA ACAGCAAAAA CAATCCCCTG CTTCCCCAGA CTCCCCCGAA 
CAGGATCCGA GTGTTCCGCA TCCAGGTGGC AAGCCGTTGG ACAGCGGACT CCAGGCCCGA 
TTGCCCAGCC AGCCGCGCGA TCTGGTGGCG CAGATAGTGA AGTCCCGATT CGTGACCCTC 
AGCTGGGTGG AACCACTCCA AAATGCCGGC GATGTAGTCT ACTACACGGT CTACTACAAG 
ATGAACAACA GCGAGAGGGA GCAAAAAATG GTTACCAAGT CCCATGACGA TCAGCAGGTT 
AATATTCAAT CGCTACTGCC CGGTAGAACC TATCAGTTCC GAGTGGAGGC CAATACCAAT 
TTCGGCAGCG GTGCATCTTC TGCTCCGCTA GAAGTTAGTA CCCAACCGGA GGTGAACATT 
GCAGGTCCGC CGCGCAACTT TGAAGGCTAT GCACGCAGTC ACAAGGAGAT CTACGTCAAA 
TGGGAAGAGC CCACGGTAAC CAATGGAGAA ATACTCAAGT ATCGAGTGTA CTATTCCGAG 
AACGACAGTG GTGCCGATCT ATATCACGAT AGTACTGCTT TGGAAGCAGT TCTTACCGAG 
TTGCGTCCCC ACACGGATTA CGTGATTTCC GTAGTGCCTT TTAATCGAAA CGGAATGGGT 
GACTCCTCAG CGGAGATACG TGTTAAGACG TTTTCGTCCA CGCCGTCGGA ACCTCCTAAT 
AATGTTACTC TCGAGGTGAC CAGCTCCAGC TCCATTACCG TCCACTGGGA GCCGCCTGCA 
GAGGAAGATC GCAATGGCCA AATTACGGGC TACAAGATTC GCTATCGCAA GTTCAAGGAT 
GCACCGCAGG TCAAGAGTAC TCCTGCCAAT ATTCGTTACT TTGAGTTGAG CAACTTGGAC 
CGCAATGCCG AGTATCAGGT GAAGATCGCT GCAATGACAG TGAACGGTTC CGGACCCTTC 
ACGGAATGGA ATCGTGCTAA CACCTTGGAA AACGATCTGG ACGAGACCCA AGTTCCGGGC 
AAACCAATTT GGATTAGCAT TCATCCTGGA GCCAACAACA TTGCCCTGCA TTGGGGTCCC 
CCACAGCATC CGGAAATCAA GATACGCAAC TACGTGCTGG GCTGGGGCCG TGGCATTCCC 
GATGAGAACA CCATAGAGCT AAAGGAGACT GAGCGCTATC ATATACTCAA GAATCTAGAA 
TCGAATATGG ATTATGTGGT GTCGCTAAGG GCTAGAAATG TTAAAGGTGA CGGTCCGCCT 
ATATATGACA ACATTAAGAC ACGCGACGAG GAGCCCGTGG ACGCACCCAC TCCACTTGAG 
GTTCCGGTCG GTCTGCGAGC CATTACCATG TCCAGCTCGT CGATTGTGGT CTACTGGATT 
GACACGATGC TGAACAAAAA TCAGCATGTA ACAGATAATC GCCACTATAC GGTTAGTTAT 
GGCATCACGG GATCCAATCG CTATCGCTAT CACAACACCA CGGATCTAAA CTGCATGATT 
AATGATCTAC GACCCAATAC GCAGTATGAG TTTGCAGTTA AAGTGGTCAA AGGACGAAGG 
GAGTCCTCCT GGTCGATGTC TGTGTTAAAT AGTACATATC AGAATGTTCC TGTCACCCCG 
CCTCGAGAGG TTACTGTGCG CTTGGATGAG ATGAATCCGC CCACTGTGAT CGTCCAGTGG 
ATACCTCCGA AACATACTCT TGGTCAGATT ACCGGATACA ATATTTACTA TACCACCGAC 
ACCACGAAAA GAGATCGCGA CTGGTCAGTT GAAGCATTTG CCGGCGAGGA AACTATGCTC 
ATGCTACCGA ATCTGAAACC TTACACAACG TATTACTTTA AGGTTCAGGC TAGAACAACC 
AAGGGGGCTA ATAATGCTCC TTTCTCGGCT CTGGTATCGT ACACCACAAG TGCAGCCGTT 
ACAATGCAGG AGCCCGACAC CATAGCCAAG GGAATCGACA ACGAGAAGCT GTTGTATATA 
ATTATAGCAG CTACCGCTGT AGTTCTACTT GTGGTGCTCT TGGGCGTCCT ATTGCTCTGC 
CGACGTAAGC CACAATCTTC GCCAGAACAC ACTAAGAAGA GCTATCAAAA GAACAATGTT 
GGCGTGCCCA AGCCACCAGA TCTATGGATA CACCATGATC AAATGGAGCT CAAAAACATA 
GACAAGGGCC TGCACACAGT GACACCCGTC TGCAGCGATG GAGCCTCGAG CAGTGGTGCC 
CTCACCCTGC CACGATCAGT GGTGCACAGT GAATACGAGG TGGAGACGCC AGTGCCCGGT 
CATGTGACCA ATTCTCTGGA CAAGCGGTCC TATGTGCCAG GATATATGAC CACATCAATG 
AACGGAACGA TGGAGCGACC TCAGTACCCG CGCACCCAGT ACAGCCACCA AAATCGTTCT 
CACATGACCA TGGAGGCTGG CCTCTCCCAG CAGAGTCTCA CCCAACCGCA AAGCAACTCG 
ATGGCTCAGA CCCCAGAGCA TCCTTATGGC GGCTACGATG CCAACTTCTG TAACGCGGGA 
AATGCAGCTG CAGGCAATGG CTGTGTGTCC ACCATTGAAA GTTCTAAGCG TGGCCATCCT 
CTAAAGAGCT TTAGTGTGCC GGGGCCACCA CCCACTGGCG GAGCCACCCC AGTTACCAAG 
CATACTCCCG CCGTCACAAT ACGTCCACAA AATCAATCGC CCTACAAGAA ACCATCTTTC 
TCGGCTGCCA CGCCCAATCG CCTGCAGGGC GGTGGCTCGG TAGTGCACTC AACCGATGAG 
ATTCAAAGAC TTGCCCCCAG CACATCTACC GAGGAGCTAA ACCAAGAAAT GGCCAATCTG 
GAGGGCCTCA TGAAGGATCT AAGCGCAATC ACGGCCAACG AGTTCGAGTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286353.1
CDS	675..5228
Translation	MAITTNRSSRTLWNWLLSSCLIFQLIGSSLASQALSFTLEPQDAVVPEGHSVLLQCAGTASIGRGGKSKSNLPSSVSIRWRGPDGQDLVIVGDTFRTQLKNGSLYISSVEENRGLTGAYQCLLTAEGVGSILSRPALVAIVRQPDLNQDFLETYLLPGQTAYFRCMLGEANWQEGVKHSVQWLKDDLPLPLDKLRMVVLPNGALEIDEVGPSDRGSYQCNVTSGSSSRLSSKTNLNIKKPSDPGAENSVAPSFLVGPSPKTVREGDTVTLDCVANGVPKPQIKWLRNGMDLDFNDLDSRFSIVGTGSLQISSAEDIDSGNYQCRASNTVDSLDAQATVQVQEPPKFIKAPKDTTAHEKDEPELKCDIWGKPKPVIRWLKNGDLITPNDYMQLVDGHNLKILGLLNSDAGMFQCVGTNAAGSVHAAARLRVVPQGGHSTKSLQTFDNLTKRRKPFNSGEQLRTKSGGSFPFPDEDDDLDDNDSGTTRLRIPSGKLPKFEQPLNDRRFNILNAGSALPLDNQSKSYEDDDDDYDEDDPAKEALLEAYKQGEDAHKILDSWQQSKSKKSQQQKQSPASPDSPEQDPSVPHPGGKPLDSGLQARLPSQPRDLVAQIVKSRFVTLSWVEPLQNAGDVVYYTVYYKMNNSEREQKMVTKSHDDQQVNIQSLLPGRTYQFRVEANTNFGSGASSAPLEVSTQPEVNIAGPPRNFEGYARSHKEIYVKWEEPTVTNGEILKYRVYYSENDSGADLYHDSTALEAVLTELRPHTDYVISVVPFNRNGMGDSSAEIRVKTFSSTPSEPPNNVTLEVTSSSSITVHWEPPAEEDRNGQITGYKIRYRKFKDAPQVKSTPANIRYFELSNLDRNAEYQVKIAAMTVNGSGPFTEWNRANTLENDLDETQVPGKPIWISIHPGANNIALHWGPPQHPEIKIRNYVLGWGRGIPDENTIELKETERYHILKNLESNMDYVVSLRARNVKGDGPPIYDNIKTRDEEPVDAPTPLEVPVGLRAITMSSSSIVVYWIDTMLNKNQHVTDNRHYTVSYGITGSNRYRYHNTTDLNCMINDLRPNTQYEFAVKVVKGRRESSWSMSVLNSTYQNVPVTPPREVTVRLDEMNPPTVIVQWIPPKHTLGQITGYNIYYTTDTTKRDRDWSVEAFAGEETMLMLPNLKPYTTYYFKVQARTTKGANNAPFSALVSYTTSAAVTMQEPDTIAKGIDNEKLLYIIIAATAVVLLVVLLGVLLLCRRKPQSSPEHTKKSYQKNNVGVPKPPDLWIHHDQMELKNIDKGLHTVTPVCSDGASSSGALTLPRSVVHSEYEVETPVPGHVTNSLDKRSYVPGYMTTSMNGTMERPQYPRTQYSHQNRSHMTMEAGLSQQSLTQPQSNSMAQTPEHPYGGYDANFCNAGNAAAGNGCVSTIESSKRGHPLKSFSVPGPPPTGGATPVTKHTPAVTIRPQNQSPYKKPSFSAATPNRLQGGGSVVHSTDEIQRLAPSTSTEELNQEMANLEGLMKDLSAITANEFEC

Target ORF information:

RefSeq Version	NM_001299424.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frazzled (fra), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299424.1

ORF Insert Sequence:

ATGGCCATCA CAACAAACAG AAGCAGCAGG ACTTTGTGGA ACTGGCTGCT CAGCAGTTGT 
CTCATATTCC AGCTGATTGG CAGCAGTTTG GCAAGTCAAG CTCTTTCGTT CACCTTGGAG 
CCCCAGGATG CCGTGGTGCC CGAGGGCCAC TCGGTGCTGC TCCAGTGTGC AGGCACTGCA 
TCCATTGGAC GTGGAGGCAA GAGCAAGTCC AACTTACCCA GCTCAGTGAG CATCCGGTGG 
CGAGGACCTG ATGGGCAGGA CCTGGTCATC GTGGGCGATA CGTTCCGCAC TCAGCTGAAG 
AATGGATCAC TGTACATTAG CTCCGTTGAG GAGAACCGTG GACTGACGGG AGCCTACCAA 
TGCCTGTTGA CTGCCGAGGG CGTGGGCAGC ATACTCAGTC GACCCGCACT GGTGGCCATT 
GTCCGACAGC CAGATCTGAA TCAGGACTTC CTGGAAACCT ACCTGCTGCC AGGTCAGACG 
GCCTACTTCC GTTGCATGCT GGGCGAAGCC AACTGGCAGG AGGGCGTTAA GCATTCGGTA 
CAGTGGCTCA AGGATGACCT GCCACTGCCC CTGGACAAAC TGCGCATGGT GGTGCTGCCC 
AACGGAGCGC TGGAGATCGA CGAAGTGGGT CCATCGGATC GCGGATCCTA TCAGTGCAAC 
GTGACTTCGG GCAGTTCCTC CCGGCTAAGC AGCAAAACGA ACTTGAACAT TAAGAAACCC 
AGTGATCCGG GTGCCGAGAA CTCAGTGGCA CCATCGTTCC TTGTGGGACC TTCGCCCAAA 
ACCGTGAGAG AGGGCGACAC CGTCACCCTG GACTGTGTGG CCAACGGAGT GCCAAAGCCG 
CAGATTAAGT GGCTGCGAAA TGGCATGGAT CTGGATTTTA ACGACCTGGA CTCACGCTTT 
TCCATCGTTG GAACGGGTTC ACTGCAAATC TCCAGTGCCG AGGACATTGA TTCAGGGAAC 
TACCAGTGCC GGGCCAGCAA TACGGTGGAT TCTCTTGATG CTCAGGCCAC GGTGCAGGTA 
CAGGAACCAC CTAAGTTCAT TAAGGCGCCC AAGGATACCA CTGCCCACGA GAAGGACGAA 
CCGGAATTGA AGTGCGATAT CTGGGGAAAG CCAAAGCCCG TAATAAGGTG GCTTAAGAAC 
GGAGATCTCA TAACGCCCAA TGACTACATG CAACTGGTGG ATGGTCACAA TCTGAAGATC 
CTCGGACTTC TCAACTCCGA CGCTGGAATG TTCCAGTGCG TGGGCACCAA TGCCGCTGGC 
AGTGTTCATG CTGCAGCTCG TCTGCGAGTT GTTCCGCAAG GAGGCCACTC CACCAAATCC 
CTTCAGACCT TTGACAATCT GACCAAGCGT CGCAAACCCT TCAATTCGGG TGAGCAGCTG 
CGAACCAAGT CCGGCGGGAG TTTTCCCTTT CCGGACGAGG ACGATGACCT AGACGACAAT 
GACAGCGGTA CGACTCGACT AAGGATCCCA TCCGGAAAGC TTCCCAAATT TGAGCAACCG 
CTCAACGACC GCAGATTCAA TATCCTAAAC GCGGGAAGTG CTTTGCCGCT GGACAACCAA 
TCGAAGAGTT ACGAGGACGA TGACGACGAC TATGACGAGG ACGATCCAGC TAAGGAGGCT 
CTCCTGGAGG CCTACAAACA GGGCGAGGAC GCACATAAAA TACTGGACTC CTGGCAGCAG 
TCCAAGAGCA AGAAATCCCA ACAGCAAAAA CAATCCCCTG CTTCCCCAGA CTCCCCCGAA 
CAGGATCCGA GTGTTCCGCA TCCAGGTGGC AAGCCGTTGG ACAGCGGACT CCAGGCCCGA 
TTGCCCAGCC AGCCGCGCGA TCTGGTGGCG CAGATAGTGA AGTCCCGATT CGTGACCCTC 
AGCTGGGTGG AACCACTCCA AAATGCCGGC GATGTAGTCT ACTACACGGT CTACTACAAG 
ATGAACAACA GCGAGAGGGA GCAAAAAATG GTTACCAAGT CCCATGACGA TCAGCAGGTT 
AATATTCAAT CGCTACTGCC CGGTAGAACC TATCAGTTCC GAGTGGAGGC CAATACCAAT 
TTCGGCAGCG GTGCATCTTC TGCTCCGCTA GAAGTTAGTA CCCAACCGGA GGTGAACATT 
GCAGGTCCGC CGCGCAACTT TGAAGGCTAT GCACGCAGTC ACAAGGAGAT CTACGTCAAA 
TGGGAAGAGC CCACGGTAAC CAATGGAGAA ATACTCAAGT ATCGAGTGTA CTATTCCGAG 
AACGACAGTG GTGCCGATCT ATATCACGAT AGTACTGCTT TGGAAGCAGT TCTTACCGAG 
TTGCGTCCCC ACACGGATTA CGTGATTTCC GTAGTGCCTT TTAATCGAAA CGGAATGGGT 
GACTCCTCAG CGGAGATACG TGTTAAGACG TTTTCGTCCA CGCCGTCGGA ACCTCCTAAT 
AATGTTACTC TCGAGGTGAC CAGCTCCAGC TCCATTACCG TCCACTGGGA GCCGCCTGCA 
GAGGAAGATC GCAATGGCCA AATTACGGGC TACAAGATTC GCTATCGCAA GTTCAAGGAT 
GCACCGCAGG TCAAGAGTAC TCCTGCCAAT ATTCGTTACT TTGAGTTGAG CAACTTGGAC 
CGCAATGCCG AGTATCAGGT GAAGATCGCT GCAATGACAG TGAACGGTTC CGGACCCTTC 
ACGGAATGGA ATCGTGCTAA CACCTTGGAA AACGATCTGG ACGAGACCCA AGTTCCGGGC 
AAACCAATTT GGATTAGCAT TCATCCTGGA GCCAACAACA TTGCCCTGCA TTGGGGTCCC 
CCACAGCATC CGGAAATCAA GATACGCAAC TACGTGCTGG GCTGGGGCCG TGGCATTCCC 
GATGAGAACA CCATAGAGCT AAAGGAGACT GAGCGCTATC ATATACTCAA GAATCTAGAA 
TCGAATATGG ATTATGTGGT GTCGCTAAGG GCTAGAAATG TTAAAGGTGA CGGTCCGCCT 
ATATATGACA ACATTAAGAC ACGCGACGAG GAGCCCGTGG ACGCACCCAC TCCACTTGAG 
GTTCCGGTCG GTCTGCGAGC CATTACCATG TCCAGCTCGT CGATTGTGGT CTACTGGATT 
GACACGATGC TGAACAAAAA TCAGCATGTA ACAGATAATC GCCACTATAC GGTTAGTTAT 
GGCATCACGG GATCCAATCG CTATCGCTAT CACAACACCA CGGATCTAAA CTGCATGATT 
AATGATCTAC GACCCAATAC GCAGTATGAG TTTGCAGTTA AAGTGGTCAA AGGACGAAGG 
GAGTCCTCCT GGTCGATGTC TGTGTTAAAT AGTACATATC AGAATGTTCC TGTCACCCCG 
CCTCGAGAGG TTACTGTGCG CTTGGATGAG ATGAATCCGC CCACTGTGAT CGTCCAGTGG 
ATACCTCCGA AACATACTCT TGGTCAGATT ACCGGATACA ATATTTACTA TACCACCGAC 
ACCACGAAAA GAGATCGCGA CTGGTCAGTT GAAGCATTTG CCGGCGAGGA AACTATGCTC 
ATGCTACCGA ATCTGAAACC TTACACAACG TATTACTTTA AGGTTCAGGC TAGAACAACC 
AAGGGGGCTA ATAATGCTCC TTTCTCGGCT CTGGTATCGT ACACCACAAG TGCAGCCGTT 
ACAATGCAGG AGCCCGACAC CATAGCCAAG GGAATCGACA ACGAGAAGCT GTTGTATATA 
ATTATAGCAG CTACCGCTGT AGTTCTACTT GTGGTGCTCT TGGGCGTCCT ATTGCTCTGC 
CGACGTAAGC CACAATCTTC GCCAGAACAC ACTAAGAAGA GCTATCAAAA GAACAATGTT 
GGCGTGCCCA AGCCACCAGA TCTATGGATA CACCATGATC AAATGGAGCT CAAAAACATA 
GACAAGGGCC TGCACACAGT GACACCCGTC TGCAGCGATG GAGCCTCGAG CAGTGGTGCC 
CTCACCCTGC CACGATCAGT GGTGCACAGT GAATACGAGG TGGAGACGCC AGTGCCCGGT 
CATGTGACCA ATTCTCTGGA CAAGCGGTCC TATGTGCCAG GATATATGAC CACATCAATG 
AACGGAACGA TGGAGCGACC TCAGTACCCG CGCACCCAGT ACAGCCACCA AAATCGTTCT 
CACATGACCA TGGAGGCTGG CCTCTCCCAG CAGAGTCTCA CCCAACCGCA AAGCAACTCG 
ATGGCTCAGA CCCCAGAGCA TCCTTATGGC GGCTACGATG CCAACTTCTG TAACGCGGGA 
AATGCAGCTG CAGGCAATGG CTGTGTGTCC ACCATTGAAA GTTCTAAGCG TGGCCATCCT 
CTAAAGAGCT TTAGTGTGCC GGGGCCACCA CCCACTGGCG GAGCCACCCC AGTTACCAAG 
CATACTCCCG CCGTCACAAT ACGTCCACAA AATCAATCGC CCTACAAGAA ACCATCTTTC 
TCGGCTGCCA CGCCCAATCG CCTGCAGGGC GGTGGCTCGG TAGTGCACTC AACCGATGAG 
ATTCAAAGAC TTGCCCCCAG CACATCTACC GAGGAGCTAA ACCAAGAAAT GGCCAATCTG 
GAGGGCCTCA TGAAGGATCT AAGCGCAATC ACGGCCAACG AGTTCGAGTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fra cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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