Nepl10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl10
Entrez Gene ID	36366
Full Name	CG8550 gene product from transcript CG8550-RA
Synonyms	Dmel\CG8550
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49A7-49A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_136943.2	NP_610787.1	Neprilysin-like 10

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4580	NP_609795.2
Drosophila melanogaster (fruit fly)	CG9505	NP_609019.1
Drosophila melanogaster (fruit fly)	CG9507	NP_609022.1
Drosophila melanogaster (fruit fly)	CG31918	NP_608904.1
Drosophila melanogaster (fruit fly)	CG8550	NP_610787.1
Drosophila melanogaster (fruit fly)	CG42370	NP_609023.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09759	NM_136943.2 Latest version!	Drosophila melanogaster Neprilysin-like 10 (Nepl10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl10 ( NM_136943.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610787.1 Drosophila melanogaster Neprilysin-like 10 (Nepl10), partial mRNA.

Price & Availability↑

CloneID	OFa09759
Clone ID Related Accession (Same CDS sequence)	NM_136943.2
Accession Version	NM_136943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136943.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGGATGTT TACTTTTACT GTGCTCTTGC CTGGTGGCAT TATTCATTCC CATCCAGGCA 
ATAGTGGACA TCAACGTGGC AACGTCGAAC GATATTCACA ATAATCTCAA TAGAACAACA 
TCACCGTGCA CGAATTTCTG GAATTTTGCC TGCGGTCGCT TCTCCAGTGC CTCTAAATAT 
GTGGACAACT TTGAGCGAGT CGAGGATCAG TTCGCCACTG CCATGGTGGA ATTCATGGAG 
AGCCACTTGG GTGAAAACGA TACACTGGCA CCCCGACTGA TAAAGCAAAT GCGACAATAC 
TACAAGGCCT GCAAGGAGGA TACGCGAAAG CTTCACGGGA ATCCTCGTCC CGAAGAACTG 
GTCAATCAGT GGATTTTGCA TCCTAAAAAA TTTCTCGTAT ACGGCTTGAA TGGTGTATTC 
ATTGATGAGC GAGTTGATGT GGCCGCCAAT GATTCCATGC GTTGGGTTAT TCAGTTAAGG 
ATGCCTGACC CGGCTGATAG ATATAGTGAC CGCCGTGTCT TGCAGCTCAT GCGCCTTTAC 
GAAGAAGCGT GGTACTCGAT GGGAGTGGTT AAACTGGTGG AGAAAATGCA TCAGCTGCAG 
GATAAGTATC GTGCAGAATA TCCTGTTGTA TATACATGGA GTTTCAGTGA ATTAAGGCAG 
GAAATCCCTA TTCCGCATTC CTTTTTTATT GAGTTATTGG GCGTGAACCA CACCGAGGAG 
CTTTATCACC TGACTTTCGA GGTGAGTGAT GTTGAGTATT TGCGGGAATG TTTTCGCTGT 
CTACGGGATT TCGCCAGTGA GCCGAAGGAC ACGTACATTC GAGCACGCCA ATATGTTTTA 
GTGGAGGAGT CGGAGCCAAG GGAGCGGAAT CCAAGAAGCT GCATTCACCA TATGCGTGCC 
TATTTACCCC TGGGCATGAA CTACATCTAT GATCGATTTA TATACCAAAA CCGGGAGGAG 
GATACCAGTA AGGTACTGGA GATTTTATCC ACTTTAAAGG CAGCTTTTGG CCAATATTTA 
GATGCCAATC GCCTGCAGCT TACGCCAAAC CAGTTGGCCT ATGTAAGAGC AAAGTTGGAA 
GGTATTCAGA TCAAAATAGG AAATTTGCCT GAGGAGAAAT CTCCTGAGTT CTATAATAAC 
CACTATGGAA GTGCGAACTT TTCCGCGACA AACTTCCTGG CCAATCTCCT TGAAGCATTT 
GCCCTACGTA CCCGTCTCCA GAATGCTGGA CTGCTTGCAA GGAATTCTCG AGTGGATCTG 
CGGCACTATT ATGTCAACGA TAACGTAAGG AAGGCTCGCA CTTCTCCCTT CTATGAGAAC 
GAAAGGAACA CCATTACTGT TCCCATGGAA TTTTTGCAGT GGCCCTTGTT CGATCACCGA 
GAGCACACCA TCTTCCAGCA CAGTCTTCTG GGCGCAGTCC TGGGCCACGA GATGAACCAC 
GCCTTTGAAC AGGAAGGTAT ACTCTTCGAT GCAGCTGGAA ATGAGTCACC TGTGGGCTTG 
GAGATCCGGG AGTCGAAGCC TTTTCGGGAT GCCATCAAGT GTGCGGAGGA GCAACCATTC 
GTCTCGCTGA AAGAACGCCT GGCAGATCTC AATGGCCTGC AACTGGCGTA CGATGCCTTC 
TTTGGCTTGG CCCACGACTC ACGGCAGTTC GAATACCGTC CATATGCCTT TGAGCAGGAG 
TTCACGGCTC CCCAACTGTT TCACCTTAGC TACGCACAGT TCTTCTGCGG ATCCCTGCCG 
CCGGTGATCG CCCACGATCG GGATGATGAG CGTGTGAACG TCAGCGTTGG CAATTTGCGT 
CAATTTGCAT ACGATTTCAA TTGCGAAACG AGTCCCTCGT CCGTCTGCGA AATGTGGCGC 
CCAGGGGATG TGGCTAATTC AAATCGAAAT GTGCATCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610787.1
CDS	1..1902
Translation	MGCLLLLCSCLVALFIPIQAIVDINVATSNDIHNNLNRTTSPCTNFWNFACGRFSSASKYVDNFERVEDQFATAMVEFMESHLGENDTLAPRLIKQMRQYYKACKEDTRKLHGNPRPEELVNQWILHPKKFLVYGLNGVFIDERVDVAANDSMRWVIQLRMPDPADRYSDRRVLQLMRLYEEAWYSMGVVKLVEKMHQLQDKYRAEYPVVYTWSFSELRQEIPIPHSFFIELLGVNHTEELYHLTFEVSDVEYLRECFRCLRDFASEPKDTYIRARQYVLVEESEPRERNPRSCIHHMRAYLPLGMNYIYDRFIYQNREEDTSKVLEILSTLKAAFGQYLDANRLQLTPNQLAYVRAKLEGIQIKIGNLPEEKSPEFYNNHYGSANFSATNFLANLLEAFALRTRLQNAGLLARNSRVDLRHYYVNDNVRKARTSPFYENERNTITVPMEFLQWPLFDHREHTIFQHSLLGAVLGHEMNHAFEQEGILFDAAGNESPVGLEIRESKPFRDAIKCAEEQPFVSLKERLADLNGLQLAYDAFFGLAHDSRQFEYRPYAFEQEFTAPQLFHLSYAQFFCGSLPPVIAHDRDDERVNVSVGNLRQFAYDFNCETSPSSVCEMWRPGDVANSNRNVHP

Target ORF information:

RefSeq Version	NM_136943.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 10 (Nepl10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136943.2

ORF Insert Sequence:

ATGGGATGTT TACTTTTACT GTGCTCTTGC CTGGTGGCAT TATTCATTCC CATCCAGGCA 
ATAGTGGACA TCAACGTGGC AACGTCGAAC GATATTCACA ATAATCTCAA TAGAACAACA 
TCACCGTGCA CGAATTTCTG GAATTTTGCC TGCGGTCGCT TCTCCAGTGC CTCTAAATAT 
GTGGACAACT TTGAGCGAGT CGAGGATCAG TTCGCCACTG CCATGGTGGA ATTCATGGAG 
AGCCACTTGG GTGAAAACGA TACACTGGCA CCCCGACTGA TAAAGCAAAT GCGACAATAC 
TACAAGGCCT GCAAGGAGGA TACGCGAAAG CTTCACGGGA ATCCTCGTCC CGAAGAACTG 
GTCAATCAGT GGATTTTGCA TCCTAAAAAA TTTCTCGTAT ACGGCTTGAA TGGTGTATTC 
ATTGATGAGC GAGTTGATGT GGCCGCCAAT GATTCCATGC GTTGGGTTAT TCAGTTAAGG 
ATGCCTGACC CGGCTGATAG ATATAGTGAC CGCCGTGTCT TGCAGCTCAT GCGCCTTTAC 
GAAGAAGCGT GGTACTCGAT GGGAGTGGTT AAACTGGTGG AGAAAATGCA TCAGCTGCAG 
GATAAGTATC GTGCAGAATA TCCTGTTGTA TATACATGGA GTTTCAGTGA ATTAAGGCAG 
GAAATCCCTA TTCCGCATTC CTTTTTTATT GAGTTATTGG GCGTGAACCA CACCGAGGAG 
CTTTATCACC TGACTTTCGA GGTGAGTGAT GTTGAGTATT TGCGGGAATG TTTTCGCTGT 
CTACGGGATT TCGCCAGTGA GCCGAAGGAC ACGTACATTC GAGCACGCCA ATATGTTTTA 
GTGGAGGAGT CGGAGCCAAG GGAGCGGAAT CCAAGAAGCT GCATTCACCA TATGCGTGCC 
TATTTACCCC TGGGCATGAA CTACATCTAT GATCGATTTA TATACCAAAA CCGGGAGGAG 
GATACCAGTA AGGTACTGGA GATTTTATCC ACTTTAAAGG CAGCTTTTGG CCAATATTTA 
GATGCCAATC GCCTGCAGCT TACGCCAAAC CAGTTGGCCT ATGTAAGAGC AAAGTTGGAA 
GGTATTCAGA TCAAAATAGG AAATTTGCCT GAGGAGAAAT CTCCTGAGTT CTATAATAAC 
CACTATGGAA GTGCGAACTT TTCCGCGACA AACTTCCTGG CCAATCTCCT TGAAGCATTT 
GCCCTACGTA CCCGTCTCCA GAATGCTGGA CTGCTTGCAA GGAATTCTCG AGTGGATCTG 
CGGCACTATT ATGTCAACGA TAACGTAAGG AAGGCTCGCA CTTCTCCCTT CTATGAGAAC 
GAAAGGAACA CCATTACTGT TCCCATGGAA TTTTTGCAGT GGCCCTTGTT CGATCACCGA 
GAGCACACCA TCTTCCAGCA CAGTCTTCTG GGCGCAGTCC TGGGCCACGA GATGAACCAC 
GCCTTTGAAC AGGAAGGTAT ACTCTTCGAT GCAGCTGGAA ATGAGTCACC TGTGGGCTTG 
GAGATCCGGG AGTCGAAGCC TTTTCGGGAT GCCATCAAGT GTGCGGAGGA GCAACCATTC 
GTCTCGCTGA AAGAACGCCT GGCAGATCTC AATGGCCTGC AACTGGCGTA CGATGCCTTC 
TTTGGCTTGG CCCACGACTC ACGGCAGTTC GAATACCGTC CATATGCCTT TGAGCAGGAG 
TTCACGGCTC CCCAACTGTT TCACCTTAGC TACGCACAGT TCTTCTGCGG ATCCCTGCCG 
CCGGTGATCG CCCACGATCG GGATGATGAG CGTGTGAACG TCAGCGTTGG CAATTTGCGT 
CAATTTGCAT ACGATTTCAA TTGCGAAACG AGTCCCTCGT CCGTCTGCGA AATGTGGCGC 
CCAGGGGATG TGGCTAATTC AAATCGAAAT GTGCATCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Nepl10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報