DUBAI cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DUBAI
Entrez Gene ID	36361
Full Name	Deubiquitinating apoptotic inhibitor
Synonyms	CG8830,Dmel\CG8830,Q7JQI1,Usp38
General protein information	Preferred Names Deubiquitinating apoptotic inhibitor Names CG8830 gene product from transcript CG8830-RB CG8830-PA CG8830-PB DUBAI-PA DUBAI-PB DeUBiquitinating-Apoptotic-Inhibitor deubiquitinating apoptotic inhibitor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49A4-49A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_165900.2	NP_725167.1	deubiquitinating apoptotic inhibitor, isoform B
NM_136940.2	NP_610784.1	deubiquitinating apoptotic inhibitor, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Usp35	NP_001170883.1
Pan troglodytes (chimpanzee)	USP35	XP_003952047.1
Gallus gallus (chicken)	USP35	XP_004938991.1
Drosophila melanogaster (fruit fly)	CG8830	NP_610784.1
Homo sapiens (human)	USP35	NP_065849.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010080	XP_319238.4
Xenopus tropicalis (tropical clawed frog)	usp35	XP_004912238.1
Canis lupus familiaris (dog)	USP35	XP_542286.3
Rattus norvegicus (Norway rat)	Usp35	XP_238899.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DUBAI interacts with DIAP1 in S2 cells, and the putative active site of its DUB domain (C367) is required to rescue DIAP1 levels following apoptotic stimuli.
Title: The deubiquitinating enzyme DUBAI stabilizes DIAP1 to suppress Drosophila apoptosis.

The following DUBAI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DUBAI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09748	NM_165900.2 Latest version!	Drosophila melanogaster deubiquitinating apoptotic inhibitor, transcript variant B (DUBAI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09748	NM_136940.2 Latest version!	Drosophila melanogaster deubiquitinating apoptotic inhibitor, transcript variant A (DUBAI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DUBAI ( NM_165900.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725167.1 Drosophila melanogaster deubiquitinating apoptotic inhibitor, transcript variant B (DUBAI), mRNA.

Price & Availability↑

CloneID	OFa09748
Clone ID Related Accession (Same CDS sequence)	NM_165900.2 , NM_136940.2
Accession Version	NM_165900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deubiquitinating apoptotic inhibitor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165900.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGTTA AGCAGAATAA CAGCGAGGCG GGTGATAATG CCTCGGATAA TGCCGGAGGA 
GCTGGCAACG GCATTCCGCC CAACGCCAAA CCCATGTCCG CGAACGGAAT GGCGAATGCC 
GAAGAGAACG TATTGCCAAA GGGCATGGAC ACGGGTTCGC CCGCTGGCGA GAGCCTGAAC 
TCCAACCGAT CGGGCAAGCG GGATATTAAG GCCCTGCTGG CATACCTCCA AAGCATCGAT 
ATGCCGGAGC AAGGCCACAA TGTGATGGCC ATCTGCGAGA GGGTTATCGT GGACTTGGCC 
CGTCTGCAAA TTCCACGAGG CGCATCCTGT CCACGCCTAA AGGAAGACGT CTGCCAGGTC 
GGTGCCTTTC TGGCTACGCG CAACGAGAAG TTCCACCAAA TGTTCTACCT GCGTGTCGTC 
TACGAGCTGA TCACCAAGTC GCCATTCGAG CCACCACCCT CGTGTGCCGT GGCCATTGTG 
TTCCAGCTGT TCGACTCCAC GCAGATCCTG GAGGCTGTAC ACTCGCTGCT GGAACAGAAT 
GTGCAGGACT CGAGCATTAA GAAGACGGTG AACCTGCTCT GTGATTGGAT CACGTACTGC 
ACCTTTTGCA GCACTCTCAA TCTTTGGGTG CTGGCGCTGC TCAAGGGGCT AAGGGATCAG 
GGCAAAATGG CGCTGCTCGA CGAGATTGCA ATGGACAACA TCGAGAAGCT GTTCCACGTG 
ATGATCTTTC CGGCGCTGAG GCAAAAGGCC GCACCGGTTG TGTTTCACAT GCTGTCAACG 
ATTAATCAGA CCCCCGAGGT TTTTCACAAG ATCTTGCCAA GGATCCCACG AGTCCTGCAG 
TATGTGAAGA ATCAGTCGAC CACCATGGAC GAGATTGGCC TGGAAACCAA GAAATGTCTG 
CAGCAACTGG TCGACCTAAC CAGCGCCCTA ATGCTACGTT TCTATGATCA GGATGAGCTC 
TATGTCGCAA CCAAGAAAGC CCTGCAGACC TATGAGCCCT CTCCGAATTG CGTGGCTCTG 
GCAAAGGCAA TGCATGAGAA TGCCCAGCCG TGGGGTCGGC GGAATGCGAG GGTTGGACTC 
GTTAACCTGG GGAATACCTG CTACATGAAT AGCGTACTTC AGGCGCTAGC CATGACCAGC 
GATTTTAGCA GGCAAATCCT GCTTATCGAG TGCAATTCAG TGCTGCTCAT GAAAGTGCAG 
CAGCAGATTG CCCTGATGCA TCACTCGCTG CGCTACGAAC TAACGCCTTC GCGAGTGCTG 
AACGCCACCC GGCCGCCTAG CTTCACGCCC GGCCTACAAC AGGACAGCTC CGAGTTCCTA 
GGATATCTGC TCGACCTGCT GCACGAGCAC GAGATCAACA GCTCCTCAGT CACTGGTCAC 
AGTGTTGGTC CTCCGAAGAC CGGCAGGGAA GTGGACGACG TGCCCGCACT GCTGAGCGAG 
GATATATTGT CGAGCGGCGT TATTCCGTAC AACAGCAAGG ATCACGAGCT CAGCAGTGGA 
AGCAACAGTG ACAATTGTAA TCACAAGCCC ACACCGACAC CACCCGCGAC TCCCACCAAA 
GCCACCAACG GCTTAAAGCA GCAGGGCCAG CAGGTTGATC AGGCGAAGCC ACCATCTACC 
ATTGACAAGA CGTTCGCTGG CAAGCTTTCC ACCACCTATC GATGTTTAAA CTGCGGCTGG 
GAGAGCCGGA ACGAGGACAG CTTTCGCGAG CTGCAGCTGT CTTTTCCCGA TGATAAGGAA 
GACTGTGGAG CCACCAACTA CTCGGTGCAG GATCTGATCG AGTACTACTG CTCGCCCGAG 
AAGCTGGACG GTGACAATCA ATATTTCTGT CCACAATGCA AGAAGCTTTG CGATGCGGAG 
CGCCACATCG GGGTCACCCA GGCACCGAAG AACCTTATTC TCACGCTCAA GCAGTTCAAG 
TACGATCAGA AGTACCACTT CCGCACCAAG CTCATGCATA AGGTGTTCCA CGATGAGAGC 
GTCACAGTAA AAATGTCGGC AAAGGACTCG CTGCAGGAGA TGTCCACTGT GCACTATGAT 
CTGTATGCGG GTGTTGTTCA CGCCGGGTAC AGCATGGATT CCGGTCACTA CTTTACGTTC 
GCGGCGGATC AAGCCAAGAA TTGGTACAAG TTCAACGATA ACGTGGTTAC CCACTCCAAG 
CCGGAGGAGA TGCACAATCT CACTTCGCCC AACACGCCCT ACATCCTGTT CTACAAGATG 
TGCGGCCACT CCAATGAGTC TAATTCGGTT ACGGCCAGCT GCAGTTCGAG TATGGTATCG 
GGCTCAAATG ACCAGGTGGT CTCGGTGCCA CTGTCGCCGC CTCTGAAGCT GGAGGAGTTG 
CCACGCCGAC TTCGCGACTA CGTAAGAAAG GACAACCATG TTTACAACGA AGAGTTGAAG 
ATGCAACGCT TTAAGCGTGG CAACAGCGGT CACGGCAATG CGTTTGTCAG CCGGCACAAC 
TTTGATGGCG ATGGTGACGA GGACGACAAG GCGCCGCCGC CGCCCGGAGG ATGTGGAGGC 
AATGGACTGG GCATGAACAT CAACCGCTTC GTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725167.1
CDS	570..3128
Translation	MAVKQNNSEAGDNASDNAGGAGNGIPPNAKPMSANGMANAEENVLPKGMDTGSPAGESLNSNRSGKRDIKALLAYLQSIDMPEQGHNVMAICERVIVDLARLQIPRGASCPRLKEDVCQVGAFLATRNEKFHQMFYLRVVYELITKSPFEPPPSCAVAIVFQLFDSTQILEAVHSLLEQNVQDSSIKKTVNLLCDWITYCTFCSTLNLWVLALLKGLRDQGKMALLDEIAMDNIEKLFHVMIFPALRQKAAPVVFHMLSTINQTPEVFHKILPRIPRVLQYVKNQSTTMDEIGLETKKCLQQLVDLTSALMLRFYDQDELYVATKKALQTYEPSPNCVALAKAMHENAQPWGRRNARVGLVNLGNTCYMNSVLQALAMTSDFSRQILLIECNSVLLMKVQQQIALMHHSLRYELTPSRVLNATRPPSFTPGLQQDSSEFLGYLLDLLHEHEINSSSVTGHSVGPPKTGREVDDVPALLSEDILSSGVIPYNSKDHELSSGSNSDNCNHKPTPTPPATPTKATNGLKQQGQQVDQAKPPSTIDKTFAGKLSTTYRCLNCGWESRNEDSFRELQLSFPDDKEDCGATNYSVQDLIEYYCSPEKLDGDNQYFCPQCKKLCDAERHIGVTQAPKNLILTLKQFKYDQKYHFRTKLMHKVFHDESVTVKMSAKDSLQEMSTVHYDLYAGVVHAGYSMDSGHYFTFAADQAKNWYKFNDNVVTHSKPEEMHNLTSPNTPYILFYKMCGHSNESNSVTASCSSSMVSGSNDQVVSVPLSPPLKLEELPRRLRDYVRKDNHVYNEELKMQRFKRGNSGHGNAFVSRHNFDGDGDEDDKAPPPPGGCGGNGLGMNINRFVF

Target ORF information:

RefSeq Version	NM_165900.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deubiquitinating apoptotic inhibitor, transcript variant B (DUBAI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165900.2

ORF Insert Sequence:

ATGGCCGTTA AGCAGAATAA CAGCGAGGCG GGTGATAATG CCTCGGATAA TGCCGGAGGA 
GCTGGCAACG GCATTCCGCC CAACGCCAAA CCCATGTCCG CGAACGGAAT GGCGAATGCC 
GAAGAGAACG TATTGCCAAA GGGCATGGAC ACGGGTTCGC CCGCTGGCGA GAGCCTGAAC 
TCCAACCGAT CGGGCAAGCG GGATATTAAG GCCCTGCTGG CATACCTCCA AAGCATCGAT 
ATGCCGGAGC AAGGCCACAA TGTGATGGCC ATCTGCGAGA GGGTTATCGT GGACTTGGCC 
CGTCTGCAAA TTCCACGAGG CGCATCCTGT CCACGCCTAA AGGAAGACGT CTGCCAGGTC 
GGTGCCTTTC TGGCTACGCG CAACGAGAAG TTCCACCAAA TGTTCTACCT GCGTGTCGTC 
TACGAGCTGA TCACCAAGTC GCCATTCGAG CCACCACCCT CGTGTGCCGT GGCCATTGTG 
TTCCAGCTGT TCGACTCCAC GCAGATCCTG GAGGCTGTAC ACTCGCTGCT GGAACAGAAT 
GTGCAGGACT CGAGCATTAA GAAGACGGTG AACCTGCTCT GTGATTGGAT CACGTACTGC 
ACCTTTTGCA GCACTCTCAA TCTTTGGGTG CTGGCGCTGC TCAAGGGGCT AAGGGATCAG 
GGCAAAATGG CGCTGCTCGA CGAGATTGCA ATGGACAACA TCGAGAAGCT GTTCCACGTG 
ATGATCTTTC CGGCGCTGAG GCAAAAGGCC GCACCGGTTG TGTTTCACAT GCTGTCAACG 
ATTAATCAGA CCCCCGAGGT TTTTCACAAG ATCTTGCCAA GGATCCCACG AGTCCTGCAG 
TATGTGAAGA ATCAGTCGAC CACCATGGAC GAGATTGGCC TGGAAACCAA GAAATGTCTG 
CAGCAACTGG TCGACCTAAC CAGCGCCCTA ATGCTACGTT TCTATGATCA GGATGAGCTC 
TATGTCGCAA CCAAGAAAGC CCTGCAGACC TATGAGCCCT CTCCGAATTG CGTGGCTCTG 
GCAAAGGCAA TGCATGAGAA TGCCCAGCCG TGGGGTCGGC GGAATGCGAG GGTTGGACTC 
GTTAACCTGG GGAATACCTG CTACATGAAT AGCGTACTTC AGGCGCTAGC CATGACCAGC 
GATTTTAGCA GGCAAATCCT GCTTATCGAG TGCAATTCAG TGCTGCTCAT GAAAGTGCAG 
CAGCAGATTG CCCTGATGCA TCACTCGCTG CGCTACGAAC TAACGCCTTC GCGAGTGCTG 
AACGCCACCC GGCCGCCTAG CTTCACGCCC GGCCTACAAC AGGACAGCTC CGAGTTCCTA 
GGATATCTGC TCGACCTGCT GCACGAGCAC GAGATCAACA GCTCCTCAGT CACTGGTCAC 
AGTGTTGGTC CTCCGAAGAC CGGCAGGGAA GTGGACGACG TGCCCGCACT GCTGAGCGAG 
GATATATTGT CGAGCGGCGT TATTCCGTAC AACAGCAAGG ATCACGAGCT CAGCAGTGGA 
AGCAACAGTG ACAATTGTAA TCACAAGCCC ACACCGACAC CACCCGCGAC TCCCACCAAA 
GCCACCAACG GCTTAAAGCA GCAGGGCCAG CAGGTTGATC AGGCGAAGCC ACCATCTACC 
ATTGACAAGA CGTTCGCTGG CAAGCTTTCC ACCACCTATC GATGTTTAAA CTGCGGCTGG 
GAGAGCCGGA ACGAGGACAG CTTTCGCGAG CTGCAGCTGT CTTTTCCCGA TGATAAGGAA 
GACTGTGGAG CCACCAACTA CTCGGTGCAG GATCTGATCG AGTACTACTG CTCGCCCGAG 
AAGCTGGACG GTGACAATCA ATATTTCTGT CCACAATGCA AGAAGCTTTG CGATGCGGAG 
CGCCACATCG GGGTCACCCA GGCACCGAAG AACCTTATTC TCACGCTCAA GCAGTTCAAG 
TACGATCAGA AGTACCACTT CCGCACCAAG CTCATGCATA AGGTGTTCCA CGATGAGAGC 
GTCACAGTAA AAATGTCGGC AAAGGACTCG CTGCAGGAGA TGTCCACTGT GCACTATGAT 
CTGTATGCGG GTGTTGTTCA CGCCGGGTAC AGCATGGATT CCGGTCACTA CTTTACGTTC 
GCGGCGGATC AAGCCAAGAA TTGGTACAAG TTCAACGATA ACGTGGTTAC CCACTCCAAG 
CCGGAGGAGA TGCACAATCT CACTTCGCCC AACACGCCCT ACATCCTGTT CTACAAGATG 
TGCGGCCACT CCAATGAGTC TAATTCGGTT ACGGCCAGCT GCAGTTCGAG TATGGTATCG 
GGCTCAAATG ACCAGGTGGT CTCGGTGCCA CTGTCGCCGC CTCTGAAGCT GGAGGAGTTG 
CCACGCCGAC TTCGCGACTA CGTAAGAAAG GACAACCATG TTTACAACGA AGAGTTGAAG 
ATGCAACGCT TTAAGCGTGG CAACAGCGGT CACGGCAATG CGTTTGTCAG CCGGCACAAC 
TTTGATGGCG ATGGTGACGA GGACGACAAG GCGCCGCCGC CGCCCGGAGG ATGTGGAGGC 
AATGGACTGG GCATGAACAT CAACCGCTTC GTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUBAI ( NM_136940.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610784.1 Drosophila melanogaster deubiquitinating apoptotic inhibitor, transcript variant A (DUBAI), mRNA.

Price & Availability↑

CloneID	OFa09748
Clone ID Related Accession (Same CDS sequence)	NM_165900.2 , NM_136940.2
Accession Version	NM_136940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deubiquitinating apoptotic inhibitor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136940.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGTTA AGCAGAATAA CAGCGAGGCG GGTGATAATG CCTCGGATAA TGCCGGAGGA 
GCTGGCAACG GCATTCCGCC CAACGCCAAA CCCATGTCCG CGAACGGAAT GGCGAATGCC 
GAAGAGAACG TATTGCCAAA GGGCATGGAC ACGGGTTCGC CCGCTGGCGA GAGCCTGAAC 
TCCAACCGAT CGGGCAAGCG GGATATTAAG GCCCTGCTGG CATACCTCCA AAGCATCGAT 
ATGCCGGAGC AAGGCCACAA TGTGATGGCC ATCTGCGAGA GGGTTATCGT GGACTTGGCC 
CGTCTGCAAA TTCCACGAGG CGCATCCTGT CCACGCCTAA AGGAAGACGT CTGCCAGGTC 
GGTGCCTTTC TGGCTACGCG CAACGAGAAG TTCCACCAAA TGTTCTACCT GCGTGTCGTC 
TACGAGCTGA TCACCAAGTC GCCATTCGAG CCACCACCCT CGTGTGCCGT GGCCATTGTG 
TTCCAGCTGT TCGACTCCAC GCAGATCCTG GAGGCTGTAC ACTCGCTGCT GGAACAGAAT 
GTGCAGGACT CGAGCATTAA GAAGACGGTG AACCTGCTCT GTGATTGGAT CACGTACTGC 
ACCTTTTGCA GCACTCTCAA TCTTTGGGTG CTGGCGCTGC TCAAGGGGCT AAGGGATCAG 
GGCAAAATGG CGCTGCTCGA CGAGATTGCA ATGGACAACA TCGAGAAGCT GTTCCACGTG 
ATGATCTTTC CGGCGCTGAG GCAAAAGGCC GCACCGGTTG TGTTTCACAT GCTGTCAACG 
ATTAATCAGA CCCCCGAGGT TTTTCACAAG ATCTTGCCAA GGATCCCACG AGTCCTGCAG 
TATGTGAAGA ATCAGTCGAC CACCATGGAC GAGATTGGCC TGGAAACCAA GAAATGTCTG 
CAGCAACTGG TCGACCTAAC CAGCGCCCTA ATGCTACGTT TCTATGATCA GGATGAGCTC 
TATGTCGCAA CCAAGAAAGC CCTGCAGACC TATGAGCCCT CTCCGAATTG CGTGGCTCTG 
GCAAAGGCAA TGCATGAGAA TGCCCAGCCG TGGGGTCGGC GGAATGCGAG GGTTGGACTC 
GTTAACCTGG GGAATACCTG CTACATGAAT AGCGTACTTC AGGCGCTAGC CATGACCAGC 
GATTTTAGCA GGCAAATCCT GCTTATCGAG TGCAATTCAG TGCTGCTCAT GAAAGTGCAG 
CAGCAGATTG CCCTGATGCA TCACTCGCTG CGCTACGAAC TAACGCCTTC GCGAGTGCTG 
AACGCCACCC GGCCGCCTAG CTTCACGCCC GGCCTACAAC AGGACAGCTC CGAGTTCCTA 
GGATATCTGC TCGACCTGCT GCACGAGCAC GAGATCAACA GCTCCTCAGT CACTGGTCAC 
AGTGTTGGTC CTCCGAAGAC CGGCAGGGAA GTGGACGACG TGCCCGCACT GCTGAGCGAG 
GATATATTGT CGAGCGGCGT TATTCCGTAC AACAGCAAGG ATCACGAGCT CAGCAGTGGA 
AGCAACAGTG ACAATTGTAA TCACAAGCCC ACACCGACAC CACCCGCGAC TCCCACCAAA 
GCCACCAACG GCTTAAAGCA GCAGGGCCAG CAGGTTGATC AGGCGAAGCC ACCATCTACC 
ATTGACAAGA CGTTCGCTGG CAAGCTTTCC ACCACCTATC GATGTTTAAA CTGCGGCTGG 
GAGAGCCGGA ACGAGGACAG CTTTCGCGAG CTGCAGCTGT CTTTTCCCGA TGATAAGGAA 
GACTGTGGAG CCACCAACTA CTCGGTGCAG GATCTGATCG AGTACTACTG CTCGCCCGAG 
AAGCTGGACG GTGACAATCA ATATTTCTGT CCACAATGCA AGAAGCTTTG CGATGCGGAG 
CGCCACATCG GGGTCACCCA GGCACCGAAG AACCTTATTC TCACGCTCAA GCAGTTCAAG 
TACGATCAGA AGTACCACTT CCGCACCAAG CTCATGCATA AGGTGTTCCA CGATGAGAGC 
GTCACAGTAA AAATGTCGGC AAAGGACTCG CTGCAGGAGA TGTCCACTGT GCACTATGAT 
CTGTATGCGG GTGTTGTTCA CGCCGGGTAC AGCATGGATT CCGGTCACTA CTTTACGTTC 
GCGGCGGATC AAGCCAAGAA TTGGTACAAG TTCAACGATA ACGTGGTTAC CCACTCCAAG 
CCGGAGGAGA TGCACAATCT CACTTCGCCC AACACGCCCT ACATCCTGTT CTACAAGATG 
TGCGGCCACT CCAATGAGTC TAATTCGGTT ACGGCCAGCT GCAGTTCGAG TATGGTATCG 
GGCTCAAATG ACCAGGTGGT CTCGGTGCCA CTGTCGCCGC CTCTGAAGCT GGAGGAGTTG 
CCACGCCGAC TTCGCGACTA CGTAAGAAAG GACAACCATG TTTACAACGA AGAGTTGAAG 
ATGCAACGCT TTAAGCGTGG CAACAGCGGT CACGGCAATG CGTTTGTCAG CCGGCACAAC 
TTTGATGGCG ATGGTGACGA GGACGACAAG GCGCCGCCGC CGCCCGGAGG ATGTGGAGGC 
AATGGACTGG GCATGAACAT CAACCGCTTC GTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610784.1
CDS	556..3114
Translation	MAVKQNNSEAGDNASDNAGGAGNGIPPNAKPMSANGMANAEENVLPKGMDTGSPAGESLNSNRSGKRDIKALLAYLQSIDMPEQGHNVMAICERVIVDLARLQIPRGASCPRLKEDVCQVGAFLATRNEKFHQMFYLRVVYELITKSPFEPPPSCAVAIVFQLFDSTQILEAVHSLLEQNVQDSSIKKTVNLLCDWITYCTFCSTLNLWVLALLKGLRDQGKMALLDEIAMDNIEKLFHVMIFPALRQKAAPVVFHMLSTINQTPEVFHKILPRIPRVLQYVKNQSTTMDEIGLETKKCLQQLVDLTSALMLRFYDQDELYVATKKALQTYEPSPNCVALAKAMHENAQPWGRRNARVGLVNLGNTCYMNSVLQALAMTSDFSRQILLIECNSVLLMKVQQQIALMHHSLRYELTPSRVLNATRPPSFTPGLQQDSSEFLGYLLDLLHEHEINSSSVTGHSVGPPKTGREVDDVPALLSEDILSSGVIPYNSKDHELSSGSNSDNCNHKPTPTPPATPTKATNGLKQQGQQVDQAKPPSTIDKTFAGKLSTTYRCLNCGWESRNEDSFRELQLSFPDDKEDCGATNYSVQDLIEYYCSPEKLDGDNQYFCPQCKKLCDAERHIGVTQAPKNLILTLKQFKYDQKYHFRTKLMHKVFHDESVTVKMSAKDSLQEMSTVHYDLYAGVVHAGYSMDSGHYFTFAADQAKNWYKFNDNVVTHSKPEEMHNLTSPNTPYILFYKMCGHSNESNSVTASCSSSMVSGSNDQVVSVPLSPPLKLEELPRRLRDYVRKDNHVYNEELKMQRFKRGNSGHGNAFVSRHNFDGDGDEDDKAPPPPGGCGGNGLGMNINRFVF

Target ORF information:

RefSeq Version	NM_136940.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deubiquitinating apoptotic inhibitor, transcript variant A (DUBAI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136940.2

ORF Insert Sequence:

ATGGCCGTTA AGCAGAATAA CAGCGAGGCG GGTGATAATG CCTCGGATAA TGCCGGAGGA 
GCTGGCAACG GCATTCCGCC CAACGCCAAA CCCATGTCCG CGAACGGAAT GGCGAATGCC 
GAAGAGAACG TATTGCCAAA GGGCATGGAC ACGGGTTCGC CCGCTGGCGA GAGCCTGAAC 
TCCAACCGAT CGGGCAAGCG GGATATTAAG GCCCTGCTGG CATACCTCCA AAGCATCGAT 
ATGCCGGAGC AAGGCCACAA TGTGATGGCC ATCTGCGAGA GGGTTATCGT GGACTTGGCC 
CGTCTGCAAA TTCCACGAGG CGCATCCTGT CCACGCCTAA AGGAAGACGT CTGCCAGGTC 
GGTGCCTTTC TGGCTACGCG CAACGAGAAG TTCCACCAAA TGTTCTACCT GCGTGTCGTC 
TACGAGCTGA TCACCAAGTC GCCATTCGAG CCACCACCCT CGTGTGCCGT GGCCATTGTG 
TTCCAGCTGT TCGACTCCAC GCAGATCCTG GAGGCTGTAC ACTCGCTGCT GGAACAGAAT 
GTGCAGGACT CGAGCATTAA GAAGACGGTG AACCTGCTCT GTGATTGGAT CACGTACTGC 
ACCTTTTGCA GCACTCTCAA TCTTTGGGTG CTGGCGCTGC TCAAGGGGCT AAGGGATCAG 
GGCAAAATGG CGCTGCTCGA CGAGATTGCA ATGGACAACA TCGAGAAGCT GTTCCACGTG 
ATGATCTTTC CGGCGCTGAG GCAAAAGGCC GCACCGGTTG TGTTTCACAT GCTGTCAACG 
ATTAATCAGA CCCCCGAGGT TTTTCACAAG ATCTTGCCAA GGATCCCACG AGTCCTGCAG 
TATGTGAAGA ATCAGTCGAC CACCATGGAC GAGATTGGCC TGGAAACCAA GAAATGTCTG 
CAGCAACTGG TCGACCTAAC CAGCGCCCTA ATGCTACGTT TCTATGATCA GGATGAGCTC 
TATGTCGCAA CCAAGAAAGC CCTGCAGACC TATGAGCCCT CTCCGAATTG CGTGGCTCTG 
GCAAAGGCAA TGCATGAGAA TGCCCAGCCG TGGGGTCGGC GGAATGCGAG GGTTGGACTC 
GTTAACCTGG GGAATACCTG CTACATGAAT AGCGTACTTC AGGCGCTAGC CATGACCAGC 
GATTTTAGCA GGCAAATCCT GCTTATCGAG TGCAATTCAG TGCTGCTCAT GAAAGTGCAG 
CAGCAGATTG CCCTGATGCA TCACTCGCTG CGCTACGAAC TAACGCCTTC GCGAGTGCTG 
AACGCCACCC GGCCGCCTAG CTTCACGCCC GGCCTACAAC AGGACAGCTC CGAGTTCCTA 
GGATATCTGC TCGACCTGCT GCACGAGCAC GAGATCAACA GCTCCTCAGT CACTGGTCAC 
AGTGTTGGTC CTCCGAAGAC CGGCAGGGAA GTGGACGACG TGCCCGCACT GCTGAGCGAG 
GATATATTGT CGAGCGGCGT TATTCCGTAC AACAGCAAGG ATCACGAGCT CAGCAGTGGA 
AGCAACAGTG ACAATTGTAA TCACAAGCCC ACACCGACAC CACCCGCGAC TCCCACCAAA 
GCCACCAACG GCTTAAAGCA GCAGGGCCAG CAGGTTGATC AGGCGAAGCC ACCATCTACC 
ATTGACAAGA CGTTCGCTGG CAAGCTTTCC ACCACCTATC GATGTTTAAA CTGCGGCTGG 
GAGAGCCGGA ACGAGGACAG CTTTCGCGAG CTGCAGCTGT CTTTTCCCGA TGATAAGGAA 
GACTGTGGAG CCACCAACTA CTCGGTGCAG GATCTGATCG AGTACTACTG CTCGCCCGAG 
AAGCTGGACG GTGACAATCA ATATTTCTGT CCACAATGCA AGAAGCTTTG CGATGCGGAG 
CGCCACATCG GGGTCACCCA GGCACCGAAG AACCTTATTC TCACGCTCAA GCAGTTCAAG 
TACGATCAGA AGTACCACTT CCGCACCAAG CTCATGCATA AGGTGTTCCA CGATGAGAGC 
GTCACAGTAA AAATGTCGGC AAAGGACTCG CTGCAGGAGA TGTCCACTGT GCACTATGAT 
CTGTATGCGG GTGTTGTTCA CGCCGGGTAC AGCATGGATT CCGGTCACTA CTTTACGTTC 
GCGGCGGATC AAGCCAAGAA TTGGTACAAG TTCAACGATA ACGTGGTTAC CCACTCCAAG 
CCGGAGGAGA TGCACAATCT CACTTCGCCC AACACGCCCT ACATCCTGTT CTACAAGATG 
TGCGGCCACT CCAATGAGTC TAATTCGGTT ACGGCCAGCT GCAGTTCGAG TATGGTATCG 
GGCTCAAATG ACCAGGTGGT CTCGGTGCCA CTGTCGCCGC CTCTGAAGCT GGAGGAGTTG 
CCACGCCGAC TTCGCGACTA CGTAAGAAAG GACAACCATG TTTACAACGA AGAGTTGAAG 
ATGCAACGCT TTAAGCGTGG CAACAGCGGT CACGGCAATG CGTTTGTCAG CCGGCACAAC 
TTTGATGGCG ATGGTGACGA GGACGACAAG GCGCCGCCGC CGCCCGGAGG ATGTGGAGGC 
AATGGACTGG GCATGAACAT CAACCGCTTC GTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DUBAI cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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