otk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	otk
Entrez Gene ID	36283
Full Name	off-track
Synonyms	CG8967,CT25769,DTrk,Dmel\CG8967,Dtkr,Dtrk,IGGtyk,OFT/TRK,OTK,Otk,Tk48D,Trk48D,anon-WO2004063362.81,dTrk
General protein information	Preferred Names off-track Names CG8967 gene product from transcript CG8967-RA CG8967-PA CG8967-PB Off-track off track otk-PA otk-PB tyrosine kinase at 48D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48D6-48D7

mRNA and Protein(s)

mRNA	Protein	Name
NM_078981.3	NP_523705.2	off-track, isoform A
NM_001299396.1	NP_001286325.1	off-track, isoform B

Pathways from BioSystems

Homologies

	PTK7	XP_002803792.1
Mus musculus (house mouse)	Ptk7	NP_780377.1
Gallus gallus (chicken)	PTK7	NP_001026206.1
Homo sapiens (human)	PTK7	NP_690621.1
Canis lupus familiaris (dog)	PTK7	XP_538929.2
Drosophila melanogaster (fruit fly)	otk	NP_523705.2
Bos taurus (cattle)	PTK7	NP_001179894.1
Xenopus tropicalis (tropical clawed frog)	LOC100487595	XP_002944477.2
Pan troglodytes (chimpanzee)	PTK7	XP_003311345.1
Danio rerio (zebrafish)	ptk7b	XP_005173100.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011489	XP_317813.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of Otk causes female sterility due to malformation of the oviduct, indicating that Otk and Otk2 are specifically involved in the sexually dimorphic development of the genital tract.
Title: The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility.

In Drosophila, Otk interacts with Wnt4 and opposes canonical Wnt signalling in embryonic patterning.
Title: PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling.

Otk is a receptor or a component of a receptor complex that recognizes a target-derived signal for R cell 1-R cell 6 axons to innervate the lamina layer
Title: The receptor tyrosine kinase Off-track is required for layer-specific neuronal connectivity in Drosophila.

The following otk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09668	NM_078981.3 Latest version!	Drosophila melanogaster off-track (otk), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21778	NM_001299396.1 Latest version!	Drosophila melanogaster off-track (otk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

otk ( NM_078981.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523705.2 Drosophila melanogaster off-track (otk), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09668
Clone ID Related Accession (Same CDS sequence)	NM_078981.3
Accession Version	NM_078981.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	off-track, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078981.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGCTA GGATGATCTC AATATGCGGC CTGGTCATGG CTTTGATGAT GGCTTCCGTT 
TTGGCCAGCT CATCGCGTTT CCAGCGAGTG CCCCAAAGTC AGTCCGTGGT GGAAAATGAA 
TCCGTGAAAT TCGAGTGCGA ATCGACCGAC TCCTACAGTG AACTGCACTA CGACTGGTTG 
CATAATGGTC ATCGGATTGC GTATGACAAG CGTGTCCATC AAATCGGTTC CAATTTGCAT 
ATCGAGGCGG TGCGGCGCAC GGAGGATGTT GGCAACTATG TTTGCATCGC GACAAATTTG 
GCCAGCGGCG CCAGGGAGGC GTCTCCGCCC GCCAAACTGA GCGTGATATA TCTTGAATCG 
GCCAGTGTTC AGCTGCTGGG CAGCAATCGC AACGAGCTGC TGCTCAAGTG CCACGTGGAG 
GGCGCCTCAG GAGATTTGGA ACCGCTAGAG ATCGAATGGT ATCGCAACAG CGAGAAACTT 
AGCACCTGGA AGAACGTCCA GCTGGATCAG CATCGCCTGA TAATCCGACA GCCGGGAAGC 
GAGGACGATG GCCTGTACCG CTGCACCGCC TCCAATGCGG CGGGTCGAGT GATGAGCAAG 
CAGGGCTATG TCTACCAGTC CAGTGTGAAG TGCCTGCCCC GACTTCCTAG GCGCAAGAAC 
GAAAAGATGA TGGAGTCCTG GGACAAACAG ACCTTCCTGT GCCGCGGTAA GCGCGGTGGA 
GCTGCTGGAC TAGAGGCACT ACCCGCCGCT CCCGAGGATC TGCGCATTGT GCAAGGACCC 
ATTGGTCAGT CGATTATCAA GGAGGGTGAG CCCACGGCTT TGACCTGTCT GTATGAACTG 
CCCGACGAGC TGAAGAACCA GCGCATTCAA CTCCGTTGGC GCAAAGATGG CAAACTTTTG 
CGTCAAGTGG AACTGGGTGG CTCGGCGCCC ATTCCAGGTC ACTCCTTCGA TTCCGGCAAG 
GATGCTCTGT TGCGTGAGGA CGCCCGTCTA GTGCTGCACA AGCAGAATGG CACCCTAAGC 
TTTGCCAGCA TTATTGCCAG TGATGCGGGT CAGTACCAGT GTCAGTTGCA GCTAGAAGCT 
CATGCTCCGA TTAACTCATC TCCTGGCATC TTGGAGGTCA TCGAGCAGCT AAAGTTTGTT 
CCTCAGCCCA CGTCCAAGAA TTTGGAGCTG GATGCAGTGG TGGCCAAGGT TCACTGCAAG 
GCGCAGGGCA CTCCAACGCC GCAGGTGCAA TGGGTTAGGG ATGGGGAAAA TACCACTTTA 
CCCGACCATG TAGAAGTGGA TGCCAATGGA ACACTGATTT TCAGAAACGT TAACTCGGAA 
CATCGCGGCA ACTACACTTG CTTGGCCACC AACAGCCAAG GACAGATAAA TGCCACTGTG 
GCTATAAATG TGGTGGTCAC TCCCAAATTT AGTGTGCCCC CAGTGGGACC CATCGAGACT 
TCGGAGCAGG GCACCGTGGT GATGCATTGC CAGGCGATTG GGGACCCCAA GCCGACAATT 
CAGTGGGACA AGGATCTAAA GTATCTGAGT GAGAACAATA CGGACCGCGA AAGATTTAGG 
TTCCTGGAGA ACGGCACTCT GGAGATACGG AATGTCCAGG TGGAGGACGA GGGTAGCTAC 
GGCTGCACAA TTGGAAATAG TGCAGGACTC AAGCGAGAGG ATGTCCAGTT GGTAGTGAAG 
ACAACCGGTG ACGGTTTCGC TCCAGAGGAA TCCGGAGGTG ATGGCTTCCT GGTCACCCGA 
GCTGTACTGA TCACCATGAC GGTGGCCCTG GCCTACATAG TGCTCGTGGT GGGCCTGATG 
CTGTGGTGTC GCTATCGCCG TCAAGCCAGA AAAGCCCGTT TAAACGATCT GAGCACAAAG 
GAAGCTGGCG GGGATCAGCC GGATGTTGCT GGCAATGGCA AAGGCTCCGA GCAGGAGCCC 
TGCCTGTCTA AGCAGCACAA TGGCCACAGC AAATCCAGAT CCAAGTCCAG TGGAGATGCC 
CAGAAATCCG ATGACACAGC CTGCAGCCAG CAGTCGAGGG CCTCAAAGAA ATCGGCACAT 
ATTTACGAGC AACTGGCTCT GCCAAGATCC GGGCTCAGTG AACTCATACA AATTGGACGC 
GGCGAGTTTG GGGATGTGTT TGTGGGCAAA CTTAAGGCTA CCTTAGTCAC CTCGCCCTCG 
GACAAGGATG CGGATACGGA AAAGCAGCAC AGCAACAGTG AAAATGGCAG TGGAGGCAGC 
GGAAGTGGAA GCACCACGCT AAGTACTCTA AATGAGAAGC GTCGCTCCAA GACCTCCATG 
GACGACATTG AGGAGATCAA GGAGGAGGAG CAGGATCAGC ACAATCAATC GGGTCTCGAG 
CAGCTCGTTC TGGTCAAAGC CCTGAATAAA GTGAAGGATG AACAGGCCTG CCAGGAGTTT 
CGACGACAGT TAGATCTGCT GCGCGCCATC TCGCACAAGG GAGTAGTACG TCTGTTTGGC 
CTGTGTCGCG AAAAGGATCC GCACTACATG GTGCTGGAGT ACACGGATTG GGGCGATCTC 
AAGCAGTTCC TGCTGGCCAC CGCCGGAAAA GTGAACACTG CCACCGCGGG CAGCTCCTCA 
CCGCCGCCAC TCACCACCAG TCAGGTTTTG GCCGTCGCCT ATCAAATTGC CCGAGGAATG 
GACGCCATCT ACAGAGCTCG CTTCACCCAT AGGGATCTGG CCACTCGCAA CTGCGTAATT 
TCCAGTGAGT TTATAGTGAA GGTATCCTAT CCGGCTCTCT GCAAGGACAA GTATAGCCGC 
GAGTACCACA AACACCGCAA CACGCTGCTC CCGATCCGCT GGCTGGCGCC CGAGTGCATC 
CAGGAAGACG AGTACACCAC CAAGAGCGAC ATCTTTGCCT ACGGTGTGGT GGTGTGGGAG 
CTCTTCAACC AGGCCACCAA GCTGCCCCAC GAGGAGCTGA CCAACGAGCA GGTGGTGCAG 
CGTTCCCAGG CGGGCTCCTT GGAGTGGTCA GTGGCCGAGG CGACGCCCGA TAGCCTGCGA 
GAGATATTGC TGTCCTGTTG GGTGTCCAAT CCGAAGGAGC GACCTTCGTT CAGCCAACTG 
GGAGCTGCTC TCAGCAAGGC GATGCAGAGT GCCGAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523705.2
CDS	918..4019
Translation	MTARMISICGLVMALMMASVLASSSRFQRVPQSQSVVENESVKFECESTDSYSELHYDWLHNGHRIAYDKRVHQIGSNLHIEAVRRTEDVGNYVCIATNLASGAREASPPAKLSVIYLESASVQLLGSNRNELLLKCHVEGASGDLEPLEIEWYRNSEKLSTWKNVQLDQHRLIIRQPGSEDDGLYRCTASNAAGRVMSKQGYVYQSSVKCLPRLPRRKNEKMMESWDKQTFLCRGKRGGAAGLEALPAAPEDLRIVQGPIGQSIIKEGEPTALTCLYELPDELKNQRIQLRWRKDGKLLRQVELGGSAPIPGHSFDSGKDALLREDARLVLHKQNGTLSFASIIASDAGQYQCQLQLEAHAPINSSPGILEVIEQLKFVPQPTSKNLELDAVVAKVHCKAQGTPTPQVQWVRDGENTTLPDHVEVDANGTLIFRNVNSEHRGNYTCLATNSQGQINATVAINVVVTPKFSVPPVGPIETSEQGTVVMHCQAIGDPKPTIQWDKDLKYLSENNTDRERFRFLENGTLEIRNVQVEDEGSYGCTIGNSAGLKREDVQLVVKTTGDGFAPEESGGDGFLVTRAVLITMTVALAYIVLVVGLMLWCRYRRQARKARLNDLSTKEAGGDQPDVAGNGKGSEQEPCLSKQHNGHSKSRSKSSGDAQKSDDTACSQQSRASKKSAHIYEQLALPRSGLSELIQIGRGEFGDVFVGKLKATLVTSPSDKDADTEKQHSNSENGSGGSGSGSTTLSTLNEKRRSKTSMDDIEEIKEEEQDQHNQSGLEQLVLVKALNKVKDEQACQEFRRQLDLLRAISHKGVVRLFGLCREKDPHYMVLEYTDWGDLKQFLLATAGKVNTATAGSSSPPPLTTSQVLAVAYQIARGMDAIYRARFTHRDLATRNCVISSEFIVKVSYPALCKDKYSREYHKHRNTLLPIRWLAPECIQEDEYTTKSDIFAYGVVVWELFNQATKLPHEELTNEQVVQRSQAGSLEWSVAEATPDSLREILLSCWVSNPKERPSFSQLGAALSKAMQSAEK

Target ORF information:

RefSeq Version	NM_078981.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster off-track (otk), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078981.3

ORF Insert Sequence:

ATGACTGCTA GGATGATCTC AATATGCGGC CTGGTCATGG CTTTGATGAT GGCTTCCGTT 
TTGGCCAGCT CATCGCGTTT CCAGCGAGTG CCCCAAAGTC AGTCCGTGGT GGAAAATGAA 
TCCGTGAAAT TCGAGTGCGA ATCGACCGAC TCCTACAGTG AACTGCACTA CGACTGGTTG 
CATAATGGTC ATCGGATTGC GTATGACAAG CGTGTCCATC AAATCGGTTC CAATTTGCAT 
ATCGAGGCGG TGCGGCGCAC GGAGGATGTT GGCAACTATG TTTGCATCGC GACAAATTTG 
GCCAGCGGCG CCAGGGAGGC GTCTCCGCCC GCCAAACTGA GCGTGATATA TCTTGAATCG 
GCCAGTGTTC AGCTGCTGGG CAGCAATCGC AACGAGCTGC TGCTCAAGTG CCACGTGGAG 
GGCGCCTCAG GAGATTTGGA ACCGCTAGAG ATCGAATGGT ATCGCAACAG CGAGAAACTT 
AGCACCTGGA AGAACGTCCA GCTGGATCAG CATCGCCTGA TAATCCGACA GCCGGGAAGC 
GAGGACGATG GCCTGTACCG CTGCACCGCC TCCAATGCGG CGGGTCGAGT GATGAGCAAG 
CAGGGCTATG TCTACCAGTC CAGTGTGAAG TGCCTGCCCC GACTTCCTAG GCGCAAGAAC 
GAAAAGATGA TGGAGTCCTG GGACAAACAG ACCTTCCTGT GCCGCGGTAA GCGCGGTGGA 
GCTGCTGGAC TAGAGGCACT ACCCGCCGCT CCCGAGGATC TGCGCATTGT GCAAGGACCC 
ATTGGTCAGT CGATTATCAA GGAGGGTGAG CCCACGGCTT TGACCTGTCT GTATGAACTG 
CCCGACGAGC TGAAGAACCA GCGCATTCAA CTCCGTTGGC GCAAAGATGG CAAACTTTTG 
CGTCAAGTGG AACTGGGTGG CTCGGCGCCC ATTCCAGGTC ACTCCTTCGA TTCCGGCAAG 
GATGCTCTGT TGCGTGAGGA CGCCCGTCTA GTGCTGCACA AGCAGAATGG CACCCTAAGC 
TTTGCCAGCA TTATTGCCAG TGATGCGGGT CAGTACCAGT GTCAGTTGCA GCTAGAAGCT 
CATGCTCCGA TTAACTCATC TCCTGGCATC TTGGAGGTCA TCGAGCAGCT AAAGTTTGTT 
CCTCAGCCCA CGTCCAAGAA TTTGGAGCTG GATGCAGTGG TGGCCAAGGT TCACTGCAAG 
GCGCAGGGCA CTCCAACGCC GCAGGTGCAA TGGGTTAGGG ATGGGGAAAA TACCACTTTA 
CCCGACCATG TAGAAGTGGA TGCCAATGGA ACACTGATTT TCAGAAACGT TAACTCGGAA 
CATCGCGGCA ACTACACTTG CTTGGCCACC AACAGCCAAG GACAGATAAA TGCCACTGTG 
GCTATAAATG TGGTGGTCAC TCCCAAATTT AGTGTGCCCC CAGTGGGACC CATCGAGACT 
TCGGAGCAGG GCACCGTGGT GATGCATTGC CAGGCGATTG GGGACCCCAA GCCGACAATT 
CAGTGGGACA AGGATCTAAA GTATCTGAGT GAGAACAATA CGGACCGCGA AAGATTTAGG 
TTCCTGGAGA ACGGCACTCT GGAGATACGG AATGTCCAGG TGGAGGACGA GGGTAGCTAC 
GGCTGCACAA TTGGAAATAG TGCAGGACTC AAGCGAGAGG ATGTCCAGTT GGTAGTGAAG 
ACAACCGGTG ACGGTTTCGC TCCAGAGGAA TCCGGAGGTG ATGGCTTCCT GGTCACCCGA 
GCTGTACTGA TCACCATGAC GGTGGCCCTG GCCTACATAG TGCTCGTGGT GGGCCTGATG 
CTGTGGTGTC GCTATCGCCG TCAAGCCAGA AAAGCCCGTT TAAACGATCT GAGCACAAAG 
GAAGCTGGCG GGGATCAGCC GGATGTTGCT GGCAATGGCA AAGGCTCCGA GCAGGAGCCC 
TGCCTGTCTA AGCAGCACAA TGGCCACAGC AAATCCAGAT CCAAGTCCAG TGGAGATGCC 
CAGAAATCCG ATGACACAGC CTGCAGCCAG CAGTCGAGGG CCTCAAAGAA ATCGGCACAT 
ATTTACGAGC AACTGGCTCT GCCAAGATCC GGGCTCAGTG AACTCATACA AATTGGACGC 
GGCGAGTTTG GGGATGTGTT TGTGGGCAAA CTTAAGGCTA CCTTAGTCAC CTCGCCCTCG 
GACAAGGATG CGGATACGGA AAAGCAGCAC AGCAACAGTG AAAATGGCAG TGGAGGCAGC 
GGAAGTGGAA GCACCACGCT AAGTACTCTA AATGAGAAGC GTCGCTCCAA GACCTCCATG 
GACGACATTG AGGAGATCAA GGAGGAGGAG CAGGATCAGC ACAATCAATC GGGTCTCGAG 
CAGCTCGTTC TGGTCAAAGC CCTGAATAAA GTGAAGGATG AACAGGCCTG CCAGGAGTTT 
CGACGACAGT TAGATCTGCT GCGCGCCATC TCGCACAAGG GAGTAGTACG TCTGTTTGGC 
CTGTGTCGCG AAAAGGATCC GCACTACATG GTGCTGGAGT ACACGGATTG GGGCGATCTC 
AAGCAGTTCC TGCTGGCCAC CGCCGGAAAA GTGAACACTG CCACCGCGGG CAGCTCCTCA 
CCGCCGCCAC TCACCACCAG TCAGGTTTTG GCCGTCGCCT ATCAAATTGC CCGAGGAATG 
GACGCCATCT ACAGAGCTCG CTTCACCCAT AGGGATCTGG CCACTCGCAA CTGCGTAATT 
TCCAGTGAGT TTATAGTGAA GGTATCCTAT CCGGCTCTCT GCAAGGACAA GTATAGCCGC 
GAGTACCACA AACACCGCAA CACGCTGCTC CCGATCCGCT GGCTGGCGCC CGAGTGCATC 
CAGGAAGACG AGTACACCAC CAAGAGCGAC ATCTTTGCCT ACGGTGTGGT GGTGTGGGAG 
CTCTTCAACC AGGCCACCAA GCTGCCCCAC GAGGAGCTGA CCAACGAGCA GGTGGTGCAG 
CGTTCCCAGG CGGGCTCCTT GGAGTGGTCA GTGGCCGAGG CGACGCCCGA TAGCCTGCGA 
GAGATATTGC TGTCCTGTTG GGTGTCCAAT CCGAAGGAGC GACCTTCGTT CAGCCAACTG 
GGAGCTGCTC TCAGCAAGGC GATGCAGAGT GCCGAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otk ( NM_001299396.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286325.1 Drosophila melanogaster off-track (otk), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21778
Clone ID Related Accession (Same CDS sequence)	NM_001299396.1
Accession Version	NM_001299396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	off-track, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGCTA GGATGATCTC AATATGCGGC CTGGTCATGG CTTTGATGAT GGCTTCCGTT 
TTGGCCAGCT CATCGCGTTT CCAGCGAGTG CCCCAAAGTC AGTCCGTGGT GGAAAATGAA 
TCCGTGAAAT TCGAGTGCGA ATCGACCGAC TCCTACAGTG AACTGCACTA CGACTGGTTG 
CATAATGGTC ATCGGATTGC GTATGACAAG CGTGTCCATC AAATCGGTTC CAATTTGCAT 
ATCGAGGCGG TGCGGCGCAC GGAGGATGTT GGCAACTATG TTTGCATCGC GACAAATTTG 
GCCAGCGGCG CCAGGGAGGC GTCTCCGCCC GCCAAACTGA GCGTGATATA TCTTGAATCG 
GCCAGTGTTC AGCTGCTGGG CAGCAATCGC AACGAGCTGC TGCTCAAGTG CCACGTGGAG 
GGCGCCTCAG GAGATTTGGA ACCGCTAGAG ATCGAATGGT ATCGCAACAG CGAGAAACTT 
AGCACCTGGA AGAACGTCCA GCTGGATCAG CATCGCCTGA TAATCCGACA GCCGGGAAGC 
GAGGACGATG GCCTGTACCG CTGCACCGCC TCCAATGCGG CGGGTCGAGT GATGAGCAAG 
CAGGGCTATG TCTACCAGTC CAGTGTGAAG TGCCTGCCCC GACTTCCTAG GCGCAAGAAC 
GAAAAGATGA TGGAGTCCTG GGACAAACAG ACCTTCCTGT GCCGCGGTAA GCGCGGTGGA 
GCTGCTGGAC TAGAGGCACT ACCCGCCGCT CCCGAGGATC TGCGCATTGT GCAAGGACCC 
ATTGGTCAGT CGATTATCAA GGAGGGTGAG CCCACGGCTT TGACCTGTCT GTATGAACTG 
CCCGACGAGC TGAAGAACCA GCGCATTCAA CTCCGTTGGC GCAAAGATGG CAAACTTTTG 
CGTCAAGTGG AACTGGGTGG CTCGGCGCCC ATTCCAGGTC ACTCCTTCGA TTCCGGCAAG 
GATGCTCTGT TGCGTGAGGA CGCCCGTCTA GTGCTGCACA AGCAGAATGG CACCCTAAGC 
TTTGCCAGCA TTATTGCCAG TGATGCGGGT CAGTACCAGT GTCAGTTGCA GCTAGAAGCT 
CATGCTCCGA TTAACTCATC TCCTGGCATC TTGGAGGTCA TCGAGCAGCT AAAGTTTGTT 
CCTCAGCCCA CGTCCAAGAA TTTGGAGCTG GATGCAGTGG TGGCCAAGGT TCACTGCAAG 
GCGCAGGGCA CTCCAACGCC GCAGGTGCAA TGGGTTAGGG ATGGGGAAAA TACCACTTTA 
CCCGACCATG TAGAAGTGGA TGCCAATGGA ACACTGATTT TCAGAAACGT TAACTCGGAA 
CATCGCGGCA ACTACACTTG CTTGGCCACC AACAGCCAAG GACAGATAAA TGCCACTGTG 
GCTATAAATG TGGTGGTCAC TCCCAAATTT AGTGTGCCCC CAGTGGGACC CATCGAGACT 
TCGGAGCAGG GCACCGTGGT GATGCATTGC CAGGCGATTG GGGACCCCAA GCCGACAATT 
CAGTGGGACA AGGATCTAAA GTATCTGAGT GAGAACAATA CGGACCGCGA AAGATTTAGG 
TTCCTGGAGA ACGGCACTCT GGAGATACGG AATGTCCAGG TGGAGGACGA GGGTAGCTAC 
GGCTGCACAA TTGGAAATAG TGCAGGACTC AAGCGAGAGG ATGTCCAGTT GGTAGTGAAG 
ACAACCGGTG ACGGTTTCGC TCCAGAGGAA TCCGGAGGTG ATGGCTTCCT GGTCACCCGA 
GCTGTACTGA TCACCATGAC GGTGGCCCTG GCCTACATAG TGCTCGTGGT GGGCCTGATG 
CTGTGGTGTC GCTATCGCCG TCAAGCCAGA AAAGCCCGTT TAAACGATCT GAGCACAAAG 
GAAGCTGGCG GGGATCAGCC GGATGTTGCT GGCAATGGCA AAGGCTCCGA GCAGGAGCCC 
TGCCTGTCTA AGCAGCACAA TGGCCACAGC AAATCCAGAT CCAAGTCCAG TGGAGATGCC 
CAGAAATCCG ATGACACAGC CTGCAGCCAG CAGTCGAGGG CCTCAAAGAA ATCGGCACAT 
ATTTACGAGC AACTGGCTCT GCCAAGATCC GGGCTCAGTG AACTCATACA AATTGGACGC 
GGCGAGTTTG GGGATGTGTT TGTGGGCAAA CTTAAGGCTA CCTTAGTCAC CTCGCCCTCG 
GACAAGGATG CGGATACGGA AAAGCAGCAC AGCAACAGTG AAAATGGCAG TGGAGGCAGC 
GGAAGTGGAA GCACCACGCT AAGTACTCTA AATGAGAAGC GTCGCTCCAA GACCTCCATG 
GACGACATTG AGGAGATCAA GGAGGAGGAG CAGGATCAGC ACAATCAATC GGGTCTCGAG 
CAGCTCGTTC TGGTCAAAGC CCTGAATAAA GTGAAGGATG AACAGGCCTG CCAGGAGTTT 
CGACGACAGT TAGATCTGCT GCGCGCCATC TCGCACAAGG GAGTAGTACG TCTGTTTGGC 
CTGTGTCGCG AAAAGGATCC GCACTACATG GTGCTGGAGT ACACGGATTG GGGCGATCTC 
AAGCAGTTCC TGCTGGCCAC CGCCGGAAAA GTGAACACTG CCACCGCGGG CAGCTCCTCA 
CCGCCGCCAC TCACCACCAG TCAGGTTTTG GCCGTCGCCT ATCAAATTGC CCGAGGAATG 
GACGCCATCT ACAGAGCTCG CTTCACCCAT AGGGATCTGG CCACTCGCAA CTGCGTAATT 
TCCAGTGAGT TTATAGTGAA GGTATCCTAT CCGGCTCTCT GCAAGGACAA GTATAGCCGC 
GAGTACCACA AACACCGCAA CACGCTGCTC CCGATCCGCT GGCTGGCGCC CGAGTGCATC 
CAGGAAGACG AGTACACCAC CAAGAGCGAC ATCTTTGCCT ACGGTGTGGT GGTGTGGGAG 
CTCTTCAACC AGGCCACCAA GCTGCCCCAC GAGGAGCTGA CCAACGAGCA GGTGGTGCAG 
CGTTCCCAGG CGGGCTCCTT GGAGTGGTCA GTGGCCGAGG CGACGCCCGA TAGCCTGCGA 
GAGATATTGT CAATGATTCG ATGGATCCTC GCTTGGCTTA GCTATGAAAT GAATGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286325.1
CDS	918..3977
Translation	MTARMISICGLVMALMMASVLASSSRFQRVPQSQSVVENESVKFECESTDSYSELHYDWLHNGHRIAYDKRVHQIGSNLHIEAVRRTEDVGNYVCIATNLASGAREASPPAKLSVIYLESASVQLLGSNRNELLLKCHVEGASGDLEPLEIEWYRNSEKLSTWKNVQLDQHRLIIRQPGSEDDGLYRCTASNAAGRVMSKQGYVYQSSVKCLPRLPRRKNEKMMESWDKQTFLCRGKRGGAAGLEALPAAPEDLRIVQGPIGQSIIKEGEPTALTCLYELPDELKNQRIQLRWRKDGKLLRQVELGGSAPIPGHSFDSGKDALLREDARLVLHKQNGTLSFASIIASDAGQYQCQLQLEAHAPINSSPGILEVIEQLKFVPQPTSKNLELDAVVAKVHCKAQGTPTPQVQWVRDGENTTLPDHVEVDANGTLIFRNVNSEHRGNYTCLATNSQGQINATVAINVVVTPKFSVPPVGPIETSEQGTVVMHCQAIGDPKPTIQWDKDLKYLSENNTDRERFRFLENGTLEIRNVQVEDEGSYGCTIGNSAGLKREDVQLVVKTTGDGFAPEESGGDGFLVTRAVLITMTVALAYIVLVVGLMLWCRYRRQARKARLNDLSTKEAGGDQPDVAGNGKGSEQEPCLSKQHNGHSKSRSKSSGDAQKSDDTACSQQSRASKKSAHIYEQLALPRSGLSELIQIGRGEFGDVFVGKLKATLVTSPSDKDADTEKQHSNSENGSGGSGSGSTTLSTLNEKRRSKTSMDDIEEIKEEEQDQHNQSGLEQLVLVKALNKVKDEQACQEFRRQLDLLRAISHKGVVRLFGLCREKDPHYMVLEYTDWGDLKQFLLATAGKVNTATAGSSSPPPLTTSQVLAVAYQIARGMDAIYRARFTHRDLATRNCVISSEFIVKVSYPALCKDKYSREYHKHRNTLLPIRWLAPECIQEDEYTTKSDIFAYGVVVWELFNQATKLPHEELTNEQVVQRSQAGSLEWSVAEATPDSLREILSMIRWILAWLSYEMNA

Target ORF information:

RefSeq Version	NM_001299396.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster off-track (otk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299396.1

ORF Insert Sequence:

ATGACTGCTA GGATGATCTC AATATGCGGC CTGGTCATGG CTTTGATGAT GGCTTCCGTT 
TTGGCCAGCT CATCGCGTTT CCAGCGAGTG CCCCAAAGTC AGTCCGTGGT GGAAAATGAA 
TCCGTGAAAT TCGAGTGCGA ATCGACCGAC TCCTACAGTG AACTGCACTA CGACTGGTTG 
CATAATGGTC ATCGGATTGC GTATGACAAG CGTGTCCATC AAATCGGTTC CAATTTGCAT 
ATCGAGGCGG TGCGGCGCAC GGAGGATGTT GGCAACTATG TTTGCATCGC GACAAATTTG 
GCCAGCGGCG CCAGGGAGGC GTCTCCGCCC GCCAAACTGA GCGTGATATA TCTTGAATCG 
GCCAGTGTTC AGCTGCTGGG CAGCAATCGC AACGAGCTGC TGCTCAAGTG CCACGTGGAG 
GGCGCCTCAG GAGATTTGGA ACCGCTAGAG ATCGAATGGT ATCGCAACAG CGAGAAACTT 
AGCACCTGGA AGAACGTCCA GCTGGATCAG CATCGCCTGA TAATCCGACA GCCGGGAAGC 
GAGGACGATG GCCTGTACCG CTGCACCGCC TCCAATGCGG CGGGTCGAGT GATGAGCAAG 
CAGGGCTATG TCTACCAGTC CAGTGTGAAG TGCCTGCCCC GACTTCCTAG GCGCAAGAAC 
GAAAAGATGA TGGAGTCCTG GGACAAACAG ACCTTCCTGT GCCGCGGTAA GCGCGGTGGA 
GCTGCTGGAC TAGAGGCACT ACCCGCCGCT CCCGAGGATC TGCGCATTGT GCAAGGACCC 
ATTGGTCAGT CGATTATCAA GGAGGGTGAG CCCACGGCTT TGACCTGTCT GTATGAACTG 
CCCGACGAGC TGAAGAACCA GCGCATTCAA CTCCGTTGGC GCAAAGATGG CAAACTTTTG 
CGTCAAGTGG AACTGGGTGG CTCGGCGCCC ATTCCAGGTC ACTCCTTCGA TTCCGGCAAG 
GATGCTCTGT TGCGTGAGGA CGCCCGTCTA GTGCTGCACA AGCAGAATGG CACCCTAAGC 
TTTGCCAGCA TTATTGCCAG TGATGCGGGT CAGTACCAGT GTCAGTTGCA GCTAGAAGCT 
CATGCTCCGA TTAACTCATC TCCTGGCATC TTGGAGGTCA TCGAGCAGCT AAAGTTTGTT 
CCTCAGCCCA CGTCCAAGAA TTTGGAGCTG GATGCAGTGG TGGCCAAGGT TCACTGCAAG 
GCGCAGGGCA CTCCAACGCC GCAGGTGCAA TGGGTTAGGG ATGGGGAAAA TACCACTTTA 
CCCGACCATG TAGAAGTGGA TGCCAATGGA ACACTGATTT TCAGAAACGT TAACTCGGAA 
CATCGCGGCA ACTACACTTG CTTGGCCACC AACAGCCAAG GACAGATAAA TGCCACTGTG 
GCTATAAATG TGGTGGTCAC TCCCAAATTT AGTGTGCCCC CAGTGGGACC CATCGAGACT 
TCGGAGCAGG GCACCGTGGT GATGCATTGC CAGGCGATTG GGGACCCCAA GCCGACAATT 
CAGTGGGACA AGGATCTAAA GTATCTGAGT GAGAACAATA CGGACCGCGA AAGATTTAGG 
TTCCTGGAGA ACGGCACTCT GGAGATACGG AATGTCCAGG TGGAGGACGA GGGTAGCTAC 
GGCTGCACAA TTGGAAATAG TGCAGGACTC AAGCGAGAGG ATGTCCAGTT GGTAGTGAAG 
ACAACCGGTG ACGGTTTCGC TCCAGAGGAA TCCGGAGGTG ATGGCTTCCT GGTCACCCGA 
GCTGTACTGA TCACCATGAC GGTGGCCCTG GCCTACATAG TGCTCGTGGT GGGCCTGATG 
CTGTGGTGTC GCTATCGCCG TCAAGCCAGA AAAGCCCGTT TAAACGATCT GAGCACAAAG 
GAAGCTGGCG GGGATCAGCC GGATGTTGCT GGCAATGGCA AAGGCTCCGA GCAGGAGCCC 
TGCCTGTCTA AGCAGCACAA TGGCCACAGC AAATCCAGAT CCAAGTCCAG TGGAGATGCC 
CAGAAATCCG ATGACACAGC CTGCAGCCAG CAGTCGAGGG CCTCAAAGAA ATCGGCACAT 
ATTTACGAGC AACTGGCTCT GCCAAGATCC GGGCTCAGTG AACTCATACA AATTGGACGC 
GGCGAGTTTG GGGATGTGTT TGTGGGCAAA CTTAAGGCTA CCTTAGTCAC CTCGCCCTCG 
GACAAGGATG CGGATACGGA AAAGCAGCAC AGCAACAGTG AAAATGGCAG TGGAGGCAGC 
GGAAGTGGAA GCACCACGCT AAGTACTCTA AATGAGAAGC GTCGCTCCAA GACCTCCATG 
GACGACATTG AGGAGATCAA GGAGGAGGAG CAGGATCAGC ACAATCAATC GGGTCTCGAG 
CAGCTCGTTC TGGTCAAAGC CCTGAATAAA GTGAAGGATG AACAGGCCTG CCAGGAGTTT 
CGACGACAGT TAGATCTGCT GCGCGCCATC TCGCACAAGG GAGTAGTACG TCTGTTTGGC 
CTGTGTCGCG AAAAGGATCC GCACTACATG GTGCTGGAGT ACACGGATTG GGGCGATCTC 
AAGCAGTTCC TGCTGGCCAC CGCCGGAAAA GTGAACACTG CCACCGCGGG CAGCTCCTCA 
CCGCCGCCAC TCACCACCAG TCAGGTTTTG GCCGTCGCCT ATCAAATTGC CCGAGGAATG 
GACGCCATCT ACAGAGCTCG CTTCACCCAT AGGGATCTGG CCACTCGCAA CTGCGTAATT 
TCCAGTGAGT TTATAGTGAA GGTATCCTAT CCGGCTCTCT GCAAGGACAA GTATAGCCGC 
GAGTACCACA AACACCGCAA CACGCTGCTC CCGATCCGCT GGCTGGCGCC CGAGTGCATC 
CAGGAAGACG AGTACACCAC CAAGAGCGAC ATCTTTGCCT ACGGTGTGGT GGTGTGGGAG 
CTCTTCAACC AGGCCACCAA GCTGCCCCAC GAGGAGCTGA CCAACGAGCA GGTGGTGCAG 
CGTTCCCAGG CGGGCTCCTT GGAGTGGTCA GTGGCCGAGG CGACGCCCGA TAGCCTGCGA 
GAGATATTGT CAATGATTCG ATGGATCCTC GCTTGGCTTA GCTATGAAAT GAATGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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otk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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