eEF1alpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eEF1alpha1
Entrez Gene ID	36271
Full Name	Elongation factor 1alpha48D
Synonyms	0248,1275,CG8280,Dm0084,DmEF1,Dmel\CG8280,EF,EF-1,EF-1[a],EF-1a,EF-1alpha,EF-1alpha F1,EF-1alphaF,EF-1alphaF1,EF-1alphaF[[1]],EF1-alpha,EF11_DROME,EF1A1,EF1alpha,EF1alpha 48D,EF1alpha48D,Ef1,Ef1a10,Ef1a48D,Ef1alpha 48D,Ef1alpha1,Ef1alpha48E,Efalpha48D,F1,F1 EF-1alpha,anon-EST:Liang-1.17,anon-EST:Liang-2.46,chr2R:7409490..7409690,chr2R:7410009..7410129,clone 1.17,clone 2.46,eEF1alpha,ef-1alpha,l(2)00248,l(2)01275,l(2)1275,l(2)248,l(2)k07303
General protein information	Preferred Names Elongation factor 1alpha48D Names CG8280 gene product from transcript CG8280-RE CG8280-PA CG8280-PC CG8280-PD CG8280-PE Ef1 alpha 48D Ef1alpha48D-PA Ef1alpha48D-PC Ef1alpha48D-PD Ef1alpha48D-PE elongation factor 1 alpha elongation factor 1 alpha 48D elongation factor 1-alpha F1 elongation factor 1alpha48D lethal (2) 00248 translational elongation factor1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48C5-48C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_165850.3	NP_725085.1	eukaryotic translation elongation factor 1 alpha 1, isoform C
NM_001299392.1	NP_001286321.1	eukaryotic translation elongation factor 1 alpha 1, isoform D
NM_001299393.1	NP_001286322.1	eukaryotic translation elongation factor 1 alpha 1, isoform E
NM_058027.5	NP_477375.1	eukaryotic translation elongation factor 1 alpha 1, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100911991	XP_003748945.1
Danio rerio (zebrafish)	eef1a1l1	NP_571338.1
Saccharomyces cerevisiae (baker's yeast)	TEF1	NP_015405.1
Magnaporthe oryzae (rice blast fungus)	MGG_03641	XP_003716248.1
Schizosaccharomyces pombe (fission yeast)	ef1a-a	NP_587757.1
Schizosaccharomyces pombe (fission yeast)	ef1a-c	NP_595255.1
Schizosaccharomyces pombe (fission yeast)	tef102	NP_594440.1
Neurospora crassa	NCU02003	XP_964868.2
Xenopus tropicalis (tropical clawed frog)	LOC101734345	XP_004914568.1
	LOC710682	XP_001103371.1
Caenorhabditis elegans (roundworm)	eef-1A.1	NP_498520.1
Caenorhabditis elegans (roundworm)	eef-1A.2	NP_509323.1
Saccharomyces cerevisiae (baker's yeast)	TEF2	NP_009676.1
Drosophila melanogaster (fruit fly)	Ef1alpha48D	NP_477375.1
Rattus norvegicus (Norway rat)	LOC100360413	XP_002730354.1
Danio rerio (zebrafish)	eef1a1l2	NP_001035074.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007406	XP_308429.3
Kluyveromyces lactis	KLLA0B08998g	XP_451929.1
Eremothecium gossypii	AGOS_ADL370C	NP_983726.1
Xenopus tropicalis (tropical clawed frog)	eef1a1	NP_001016692.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Efficient binding of ZADs from the proteins Grau, ZIPIC, and Zw5 to the translation elongation factor EF1alpha1 in nuclear and cytoplasmic extracts has been demonstrated.
Title: [Translation elongation factor EF1?1 interacts with ZAD domains of transcription factors from Drosophila melanogaster].

The following eEF1alpha1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eEF1alpha1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09653	NM_058027.5 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09653	NM_001299392.1 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09653	NM_165850.3 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09653	NM_001299393.1 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eEF1alpha1 ( NM_165850.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725085.1 Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa09653
Clone ID Related Accession (Same CDS sequence)	NM_165850.3 , NM_001299392.1 , NM_001299393.1 , NM_058027.5
Accession Version	NM_165850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 1 alpha 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165850.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725085.1
CDS	91..1482
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSSEPPYSEARYEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMPWFKGWKVERKEGNADGKTLIDALDAILPPARPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGTVVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKANPPKGAADFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFAEIKEKVDRRSGKTTEENPKFIKSGDAAIVNLVPSKPLCVEAFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKDASGGKVTKAAEKATKGKK

Target ORF information:

RefSeq Version	NM_165850.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165850.3

ORF Insert Sequence:

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eEF1alpha1 ( NM_001299392.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286321.1 Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa09653
Clone ID Related Accession (Same CDS sequence)	NM_165850.3 , NM_001299392.1 , NM_001299393.1 , NM_058027.5
Accession Version	NM_001299392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 1 alpha 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286321.1
CDS	34..1425
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSSEPPYSEARYEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMPWFKGWKVERKEGNADGKTLIDALDAILPPARPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGTVVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKANPPKGAADFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFAEIKEKVDRRSGKTTEENPKFIKSGDAAIVNLVPSKPLCVEAFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKDASGGKVTKAAEKATKGKK

Target ORF information:

RefSeq Version	NM_001299392.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299392.1

ORF Insert Sequence:

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eEF1alpha1 ( NM_001299393.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286322.1 Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa09653
Clone ID Related Accession (Same CDS sequence)	NM_165850.3 , NM_001299392.1 , NM_001299393.1 , NM_058027.5
Accession Version	NM_001299393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 1 alpha 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286322.1
CDS	131..1522
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSSEPPYSEARYEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMPWFKGWKVERKEGNADGKTLIDALDAILPPARPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGTVVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKANPPKGAADFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFAEIKEKVDRRSGKTTEENPKFIKSGDAAIVNLVPSKPLCVEAFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKDASGGKVTKAAEKATKGKK

Target ORF information:

RefSeq Version	NM_001299393.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299393.1

ORF Insert Sequence:

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eEF1alpha1 ( NM_058027.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477375.1 Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant A, mRNA.

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CloneID	OFa09653
Clone ID Related Accession (Same CDS sequence)	NM_165850.3 , NM_001299392.1 , NM_001299393.1 , NM_058027.5
Accession Version	NM_058027.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 1 alpha 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_058027.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477375.1
CDS	84..1475
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSSEPPYSEARYEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMPWFKGWKVERKEGNADGKTLIDALDAILPPARPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGTVVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKANPPKGAADFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFAEIKEKVDRRSGKTTEENPKFIKSGDAAIVNLVPSKPLCVEAFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKDASGGKVTKAAEKATKGKK

Target ORF information:

RefSeq Version	NM_058027.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 1 alpha 1 (eEF1alpha1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058027.5

ORF Insert Sequence:

ATGGGCAAGG AAAAGATTCA CATTAACATT GTCGTGATCG GACACGTCGA TTCCGGTAAG 
TCGACCACCA CCGGACACTT GATCTACAAG TGCGGTGGTA TCGACAAGCG TACCATCGAG 
AAGTTCGAGA AGGAGGCCCA GGAGATGGGA AAGGGATCCT TCAAGTACGC CTGGGTTTTG 
GATAAGTTGA AGGCTGAGCG CGAGCGTGGT ATCACCATCG ATATCGCCCT GTGGAAGTTC 
GAAACTGCCA AGTACTACGT GACCATCATT GATGCCCCCG GACACAGGGA TTTCATCAAG 
AACATGATCA CTGGTACCTC GCAGGCCGAT TGCGCCGTGC TGATTGTCGC CGCCGGAACC 
GGAGAATTCG AGGCAGGTAT CTCGAAGAAC GGCCAGACCC GCGAGCACGC CCTGCTCGCC 
TTCACCCTGG GTGTGAAGCA GCTGATCGTT GGTGTGAACA AGATGGACTC CTCCGAGCCA 
CCATACAGCG AGGCCCGTTA TGAGGAAATC AAGAAGGAAG TGTCCTCTTA CATCAAGAAG 
ATCGGCTACA ACCCAGCCGC CGTTGCCTTC GTGCCCATTT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCTACCAA CATGCCCTGG TTCAAGGGAT GGAAGGTGGA GCGCAAGGAG 
GGTAACGCTG ACGGCAAGAC CCTGATCGAT GCCCTCGATG CCATCCTTCC CCCAGCCCGT 
CCCACCGACA AGGCCCTGCG TCTGCCCCTG CAGGATGTGT ACAAAATTGG CGGTATTGGA 
ACAGTACCCG TGGGTCGTGT GGAGACTGGT GTGCTGAAGC CCGGTACCGT TGTGGTCTTC 
GCCCCTGCTA ACATCACCAC TGAGGTCAAG TCCGTGGAGA TGCACCACGA GGCCCTGCAG 
GAGGCCGTTC CCGGAGACAA CGTTGGCTTC AACGTCAAGA ACGTGTCCGT GAAGGAGCTG 
CGTCGTGGCT ACGTTGCCGG TGACTCCAAG GCTAACCCCC CCAAGGGAGC CGCCGACTTC 
ACCGCCCAGG TCATCGTGCT GAACCACCCC GGTCAGATTG CCAACGGCTA CACCCCCGTG 
TTGGATTGCC ACACCGCTCA CATTGCTTGC AAGTTCGCTG AGATCAAGGA GAAGGTCGAC 
CGTCGTTCCG GCAAGACCAC CGAGGAGAAC CCCAAGTTCA TCAAGTCTGG CGATGCTGCC 
ATCGTCAACC TGGTGCCCTC TAAGCCCCTG TGCGTGGAGG CCTTCCAGGA GTTCCCCCCT 
CTGGGTCGCT TCGCTGTGCG TGACATGAGG CAGACCGTGG CTGTCGGTGT CATTAAGGCT 
GTCAACTTCA AGGATGCCTC CGGTGGCAAG GTCACCAAGG CCGCCGAGAA GGCCACCAAG 
GGCAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eEF1alpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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