Buffy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Buffy
Entrez Gene ID	36251
Full Name	CG8238 gene product from transcript CG8238-RA
Synonyms	BUFFY,Bcl-2-48AE,Buffy/Borg2,Buffy/DBorg-2,Buffy/dBorg-2,CG8238,Dbx,Dmel\CG8238,Drob2,buffy,dBORG-2/BUFFY,dBorg-2,dBorg2,dbuffy
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48C1-48C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078978.2	NP_523702.1	buffy

Pathways from BioSystems

Homologies

Homo sapiens (human)	BOK	NP_115904.1
Xenopus tropicalis (tropical clawed frog)	bok	XP_002936655.1
Canis lupus familiaris (dog)	BOK	XP_003639944.1
Danio rerio (zebrafish)	boka	NP_001003612.1
Pan troglodytes (chimpanzee)	BOK	XP_003309630.2
	BOK	XP_001093115.1
Bos taurus (cattle)	BOK	NP_001092338.1
Rattus norvegicus (Norway rat)	Bok	NP_059008.1
Gallus gallus (chicken)	BOK	NP_990037.1
Drosophila melanogaster (fruit fly)	Buffy	NP_523702.1
Mus musculus (house mouse)	Bok	NP_058058.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Buffy has a role enhancing the loss of parkin and suppressing the loss of Pink1 phenotypes in Drosophila
Title: Overexpression of Buffy enhances the loss of parkin and suppresses the loss of Pink1 phenotypes in Drosophila.

The inhibition of porin phenocopies Parkinson disease phenotypes in Drosophila, while the overexpression of Buffy can counteract these phenotypes to improve the overall "healthspan" of the organism.
Title: Loss of porin function in dopaminergic neurons of Drosophila is suppressed by Buffy.

Buffy inhibition results in shortened lifespan and impaired locomotor function and represents a novel model of Parkinson disease in Drosophila melanogaster. The overexpression of Buffy improves healthspan and counteracts the effects of alpha-synuclein expression to demonstrate its protective function.
Title: The Bcl-2 homologue Buffy rescues ?-synuclein-induced Parkinson disease-like phenotypes in Drosophila.

The buffy null mutant reacted to starvation with the expected responses such as inhibition of target of rapamycin (Tor) signaling, autophagy initiation and mobilization of stored lipids.
Title: Drosophila larvae lacking the bcl-2 gene, buffy, are sensitive to nutrient stress, maintain increased basal target of rapamycin (Tor) signaling and exhibit characteristics of altered basal energy metabolism.

Developmental programmed cell death regulation in the fly does not rely upon the Bcl-2 proteins.
Title: Drosophila Bcl-2 proteins participate in stress-induced apoptosis, but are not required for normal development.

The following Buffy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Buffy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09635	NM_078978.2 Latest version!	Drosophila melanogaster buffy (Buffy), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Buffy ( NM_078978.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523702.1 Drosophila melanogaster buffy (Buffy), partial mRNA.

Price & Availability↑

CloneID	OFa09635
Clone ID Related Accession (Same CDS sequence)	NM_078978.2
Accession Version	NM_078978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	buffy
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078978.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end.

ATGCCCGGCA CCTCGTATCC CACGAATAAC GATAATTTCA GCAACGGATT TCCGATGGCC 
ACCACACAAA GTGAGCGCCT CTTGCAGGCC CAGAATCGAA GAAAGTTCAG TTTTCCAGCC 
ACACTACATT CCGCATCACT ACTGGAGGTT GGTGGTGGTC CCAAGGAGAC CACGAGGCGT 
AGGTTGAGTA ATGTCAGCGA TGCCGTCACC AGGAAACTCT CCTACACGAT TGGCTGGAAG 
GCGGCACAGA TTCCAGCGCA GGATATCATT TCTCAGGGTC GTTGCCTGTG CGGTCATTAT 
ATCAAGAGAC GCCTCAGAAG ATCTGGTTTG TTCAACAAAA AGCTGGGACT GCAGCGCATA 
CGTAGCATAC TGGGCTCCAC CTCCATGGGC ATTGTGCGCG ATGTGTTTCC AGCGGTCCAA 
GTGCTGGGCG ATGAACTGGA GCGCATGCAT CCCCGGATAT ACAACGGAGT AGCTCGTCAG 
ATTTGCCGGA ATCCGGGTGG TGAGTTCCAT ACTCCGGATG CTGTGAGCTT GCTTTTGGGA 
GCCGTTGGTC GCGAGCTCTT CCGGGTGGAG ATTACGTGGA GCAAGGTGAT CTCTCTGTTC 
GCCATCGCCG GTGGGCTGTC CGTTGATTGT GTACGCCAAG GACATCCGGA ATACTTGCCC 
AAGTTGATGG AGAGCGTTTC CGAGGTGATC GAAGATGAGC TGGTTCCGTG GATTAACGAG 
AATGGTGGTT GGAGTGGCAT CAACACACAC GTTTTACCCA CTACCAACAG CCTGAATCCT 
TTAGAGTGGA CCACTTTGGT TATAGGCGTA GTTTTTGGCC TAATTCTAGT TTTTATGATT 
CTGCGATTTA TATTTAACTT GATTGTACCA AAAATATACC AACGATTTAC GAATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523702.1
CDS	139..1038
Translation	MPGTSYPTNNDNFSNGFPMATTQSERLLQAQNRRKFSFPATLHSASLLEVGGGPKETTRRRLSNVSDAVTRKLSYTIGWKAAQIPAQDIISQGRCLCGHYIKRRLRRSGLFNKKLGLQRIRSILGSTSMGIVRDVFPAVQVLGDELERMHPRIYNGVARQICRNPGGEFHTPDAVSLLLGAVGRELFRVEITWSKVISLFAIAGGLSVDCVRQGHPEYLPKLMESVSEVIEDELVPWINENGGWSGINTHVLPTTNSLNPLEWTTLVIGVVFGLILVFMILRFIFNLIVPKIYQRFTNS

Target ORF information:

RefSeq Version	NM_078978.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster buffy (Buffy), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078978.2

ORF Insert Sequence:

ATGCCCGGCA CCTCGTATCC CACGAATAAC GATAATTTCA GCAACGGATT TCCGATGGCC 
ACCACACAAA GTGAGCGCCT CTTGCAGGCC CAGAATCGAA GAAAGTTCAG TTTTCCAGCC 
ACACTACATT CCGCATCACT ACTGGAGGTT GGTGGTGGTC CCAAGGAGAC CACGAGGCGT 
AGGTTGAGTA ATGTCAGCGA TGCCGTCACC AGGAAACTCT CCTACACGAT TGGCTGGAAG 
GCGGCACAGA TTCCAGCGCA GGATATCATT TCTCAGGGTC GTTGCCTGTG CGGTCATTAT 
ATCAAGAGAC GCCTCAGAAG ATCTGGTTTG TTCAACAAAA AGCTGGGACT GCAGCGCATA 
CGTAGCATAC TGGGCTCCAC CTCCATGGGC ATTGTGCGCG ATGTGTTTCC AGCGGTCCAA 
GTGCTGGGCG ATGAACTGGA GCGCATGCAT CCCCGGATAT ACAACGGAGT AGCTCGTCAG 
ATTTGCCGGA ATCCGGGTGG TGAGTTCCAT ACTCCGGATG CTGTGAGCTT GCTTTTGGGA 
GCCGTTGGTC GCGAGCTCTT CCGGGTGGAG ATTACGTGGA GCAAGGTGAT CTCTCTGTTC 
GCCATCGCCG GTGGGCTGTC CGTTGATTGT GTACGCCAAG GACATCCGGA ATACTTGCCC 
AAGTTGATGG AGAGCGTTTC CGAGGTGATC GAAGATGAGC TGGTTCCGTG GATTAACGAG 
AATGGTGGTT GGAGTGGCAT CAACACACAC GTTTTACCCA CTACCAACAG CCTGAATCCT 
TTAGAGTGGA CCACTTTGGT TATAGGCGTA GTTTTTGGCC TAATTCTAGT TTTTATGATT 
CTGCGATTTA TATTTAACTT GATTGTACCA AAAATATACC AACGATTTAC GAATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Buffy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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