CG12325 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12325
Entrez Gene ID	36144
Full Name	CG12325 gene product from transcript CG12325-RB
Synonyms	Dmel\CG12325
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47C1-47C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_136774.2	NP_610618.1	uncharacterized protein, isoform A
NM_001299367.1	NP_001286296.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PWP2	XP_531577.2
	PWP2	XP_001118393.2
Mus musculus (house mouse)	Pwp2	NP_083822.1
Gallus gallus (chicken)	PWP2	XP_416757.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010693	XP_311407.4
Saccharomyces cerevisiae (baker's yeast)	PWP2	NP_009984.1
Eremothecium gossypii	AGOS_AAR057W	NP_982598.1
Arabidopsis thaliana (thale cress)	PWP2	NP_172998.1
Oryza sativa (rice)	Os05g0519500	NP_001056064.1
Rattus norvegicus (Norway rat)	Pwp2	NP_001162124.1
Drosophila melanogaster (fruit fly)	CG12325	NP_610618.1
Caenorhabditis elegans (roundworm)	F55F8.3	NP_491653.2
Neurospora crassa	NCU03794	XP_961183.1
Xenopus tropicalis (tropical clawed frog)	LOC100158563	NP_001121467.1
Danio rerio (zebrafish)	pwp2h	NP_998212.1
Kluyveromyces lactis	KLLA0C08976g	XP_452599.1
Canis lupus familiaris (dog)	PWP2	XP_544915.4
Bos taurus (cattle)	PWP2	XP_002685190.1
Schizosaccharomyces pombe (fission yeast)	SPBC713.04c	NP_595343.1
Magnaporthe oryzae (rice blast fungus)	MGG_07465	XP_003711369.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12325 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12325 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12917	NM_136774.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12325), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12917	NM_001299367.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12325), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12325 ( NM_136774.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610618.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12325), mRNA.

Price & Availability↑

CloneID	OFa12917
Clone ID Related Accession (Same CDS sequence)	NM_136774.2 , NM_001299367.1
Accession Version	NM_136774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136774.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTT CCTACAAATT TTCCAACCTG TTGGGCACAA TATATCGTAA TGGAAACTTG 
GTGTTCACAC CGGATGGCAA TTCCGTTATC AGTCCGGTTG GCAATAGGAT AACGGTCTAC 
GATTTAAAAA ACAACAAATC GCGCACCTTG TCCCTGGAAT CGCGGTATAA CTACACGAAC 
ATAGCTCTTT CCCCGGATGG AAGTCTGCTC ATCGCAGTCA ATGAAAAGGG TGAGGCGCAG 
CTCATCAGCA TGATGTCCTG TACGGTGATA CATCGTCACA AGTTCCAGAC GGGCGTACAG 
TGCATCTGCT TCAGTCCAAA TGGAGCATTC TTTGCCGTCG CTGTGCAGAA CGTAGTGTTG 
ATATTCTGTG CACCTGGTGA GATCACAGGG GAGTACAATC CTTTCGTCCT GCGCCGAAGA 
TTCCTAGGTG GATTCGACGA TGTCACTTGG CTGGACTGGT CTTCGGACTC TCGACTTTTG 
GCAATCGGCT GTCGGGATAG CTCCACCAAA ATCGTTGCCA TCAACTATAC AAAGAACTTC 
CGCACCTATA ACCTAAGTGG CCACACAGAC GCCATTGTAG CTTGTTTTTT CGAGGCAAAC 
AGCTTGCACT TGAACACCAT AAGCCGCAAT GGTCAGCTCT GCTTGTGGGA ATGCAGCCTA 
GCTCCCTCGG ATTTAGAGGA TGCTGAAAGG CCAAAAATCG CTCCCCCTCA AAGGAAGAAG 
GAAAAGAAGG CAGTATCTGA CAATGAGTCA GAAGATGACG TTGAAAATGT AGTGGAAAAG 
GATGCGTTGA AGGAGGAATC TACCAAGGAA GCCGAAGATG TCAACCATAT AAAAAATGAG 
GACGAGACGA AGAGTCACCC CTTCTTTTAC AAAAAAATAA CTCGTCATTA CTTGGCCGAT 
GAGCCGCGAA AAGAACAGCG GGATGTACAA CTGACCGCGG CAAACTACAA CACTCGTACC 
AAGGTTTTGG TGGTAGCCTT CAGTTCCGGA GCTTTCTACC TCTACGAATT GCCCGACGTC 
AACATGATTC ATTCGCTGAG CATTTCCGAC TATCCTATTT CGGCGGCTCT CTTCAACTGC 
ACTGGTGACT GGGTCGCATT GGCCTCCCGA GAGATTGGCC AACTTCTGGT TTGGGAGTGG 
CAAAGCGAGC AATACATAAT GAAGCAGCAG GGACACAGCA GCGAAATGAC CTGCATTGCT 
TACTCCTCGG ATGGTCAGTT CATCGCCACT GGCGGCGAAG ACTCCAAGGT GAAGCTCTGG 
AACACGCAGA GCAGTTTCTG TTTTGTTACT TTCAGCGAGC ACACCAGTGG AGTTACTGGC 
ATACAGTTCA GCAGGAACAA GAAGTTCCTG GTTAGCAGTT CCCTAGACGG CACAGTGCGT 
GCTTTTGATG TCAACAGATA CCGCAACTTC AGGACTTTCA CCTCACCGAA TCCTGCTCAG 
TTCTCCTGTG TAGCTGTGGA TTACTCTGGC GAGTTAGTTG TGGCTGGCGG CCAGGACGTA 
TTCGATATCT TCCTGTGGTC AGTTAAAACA GGAAAGCTGC TGGAGATCAT CAGTGGCCAT 
GAGGGACCCG TAGTTTCCAT TGCATTCTCA CCAGTGGCTA CTTCATCAAC TTTGGTGTCT 
GGTTCTTGGG ACAAGACCGT CAAGATATGG AACTGCCTGG AGAGCAACAG CGAGCACGAA 
ACTATTGATG CTGTTTCAGA TGTTACAAAT GTTACTTTCA GTCCTAGTGG TGAAGAGATT 
GCTGTGGCCA CTCTCAGTGG TAATATAACA ATTTTCGACA TCAAATCTGC TGGCCAAGTT 
ACTACTATAG AGGGTCGCAA TGATCTGAGC GCAGGGCGCC TGGAAACCGA TATTATAACA 
GCGAGGAAAA ATGCTCAGGC AAACTATTTT TCCACCATTG AGTACTCAGC GGATGGAGAG 
TGCATACTGG CGGCTGGAAA GTCTGCAAAC ATATGCATTT ACCATGTTAG GGAGGCAATA 
CTTTTGAAGA AATTTGAGAT CACACAAAAC CACAGCTTAG ATGGCCTTAA TGACTTTATC 
AGTCGAAAAC ATTTAAGTGA ATTTGGCAAC ATGGCTCTTG TGGAAGAACG AGAAGAGCTG 
GAGGGCGGCA GGGTGGCTAT TCGATTGCCG GGCGTACAAC GGGGAGACAT GTCATCCAGG 
AGATTCCAGC AAGAGGTTAG GGTGTTCTCC GTAAAGTTCT CACCCACAGG CCAGGCATTT 
GCGGCTGCTG GCACTGAAGG ACTATGCATA TACGCCTTAG ATAAGGGTGT TGTCTTCGAT 
CCCTTTGATC TCTCGCTGGA AGTTACGCCG AAAGCGGTCC ACGAGTCCCT CAAGCAGCAG 
AATTACACCA AGGCGTTGGT CATGTCCCTC AAGCTAAACG AGCCCAATTT GATTGCCCTA 
GTGCTGGAGC GAGTGCCGTA TAAGGACGTC GAACTGGTTT GTGCGGATCT GTCGCCGGAG 
TTTGCCCAGC GACTGTTGCA ACAATTGGCC CGTCAGCTCC AGTCCACGCC ACACATAGAG 
TTCTATCTGC AATGGAGCTG CTGTCTGCTG ACCAAACACG GAAACCAAGA TGGAGTCTTT 
CAGCACACGG GCTTGCTGGC ACTGCACGAG GTTCTTTCGA GGAAATACGA AATGCTTAAC 
AAAATCTGCG ACTACAACAA GTACACGTTG AAAGTACTCC TGGATAGAGC TGATAAGTTG 
GAGCAGAAAA ACGAGGGCAA ACCCAATGCG ACGGAGGAAG ATAGTGATGA GGAAATGCTA 
CTTATCAGGT CGAAGGACGA CGAAAATGGA CAGGCGCAGT TTAGCGATGA GGAGGAGAGC 
GACAGCGGAT CCGAAGAAGA GATGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610618.1
CDS	106..2955
Translation	MKFSYKFSNLLGTIYRNGNLVFTPDGNSVISPVGNRITVYDLKNNKSRTLSLESRYNYTNIALSPDGSLLIAVNEKGEAQLISMMSCTVIHRHKFQTGVQCICFSPNGAFFAVAVQNVVLIFCAPGEITGEYNPFVLRRRFLGGFDDVTWLDWSSDSRLLAIGCRDSSTKIVAINYTKNFRTYNLSGHTDAIVACFFEANSLHLNTISRNGQLCLWECSLAPSDLEDAERPKIAPPQRKKEKKAVSDNESEDDVENVVEKDALKEESTKEAEDVNHIKNEDETKSHPFFYKKITRHYLADEPRKEQRDVQLTAANYNTRTKVLVVAFSSGAFYLYELPDVNMIHSLSISDYPISAALFNCTGDWVALASREIGQLLVWEWQSEQYIMKQQGHSSEMTCIAYSSDGQFIATGGEDSKVKLWNTQSSFCFVTFSEHTSGVTGIQFSRNKKFLVSSSLDGTVRAFDVNRYRNFRTFTSPNPAQFSCVAVDYSGELVVAGGQDVFDIFLWSVKTGKLLEIISGHEGPVVSIAFSPVATSSTLVSGSWDKTVKIWNCLESNSEHETIDAVSDVTNVTFSPSGEEIAVATLSGNITIFDIKSAGQVTTIEGRNDLSAGRLETDIITARKNAQANYFSTIEYSADGECILAAGKSANICIYHVREAILLKKFEITQNHSLDGLNDFISRKHLSEFGNMALVEEREELEGGRVAIRLPGVQRGDMSSRRFQQEVRVFSVKFSPTGQAFAAAGTEGLCIYALDKGVVFDPFDLSLEVTPKAVHESLKQQNYTKALVMSLKLNEPNLIALVLERVPYKDVELVCADLSPEFAQRLLQQLARQLQSTPHIEFYLQWSCCLLTKHGNQDGVFQHTGLLALHEVLSRKYEMLNKICDYNKYTLKVLLDRADKLEQKNEGKPNATEEDSDEEMLLIRSKDDENGQAQFSDEEESDSGSEEEMA

Target ORF information:

RefSeq Version	NM_136774.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12325), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136774.2

ORF Insert Sequence:

ATGAAGTTTT CCTACAAATT TTCCAACCTG TTGGGCACAA TATATCGTAA TGGAAACTTG 
GTGTTCACAC CGGATGGCAA TTCCGTTATC AGTCCGGTTG GCAATAGGAT AACGGTCTAC 
GATTTAAAAA ACAACAAATC GCGCACCTTG TCCCTGGAAT CGCGGTATAA CTACACGAAC 
ATAGCTCTTT CCCCGGATGG AAGTCTGCTC ATCGCAGTCA ATGAAAAGGG TGAGGCGCAG 
CTCATCAGCA TGATGTCCTG TACGGTGATA CATCGTCACA AGTTCCAGAC GGGCGTACAG 
TGCATCTGCT TCAGTCCAAA TGGAGCATTC TTTGCCGTCG CTGTGCAGAA CGTAGTGTTG 
ATATTCTGTG CACCTGGTGA GATCACAGGG GAGTACAATC CTTTCGTCCT GCGCCGAAGA 
TTCCTAGGTG GATTCGACGA TGTCACTTGG CTGGACTGGT CTTCGGACTC TCGACTTTTG 
GCAATCGGCT GTCGGGATAG CTCCACCAAA ATCGTTGCCA TCAACTATAC AAAGAACTTC 
CGCACCTATA ACCTAAGTGG CCACACAGAC GCCATTGTAG CTTGTTTTTT CGAGGCAAAC 
AGCTTGCACT TGAACACCAT AAGCCGCAAT GGTCAGCTCT GCTTGTGGGA ATGCAGCCTA 
GCTCCCTCGG ATTTAGAGGA TGCTGAAAGG CCAAAAATCG CTCCCCCTCA AAGGAAGAAG 
GAAAAGAAGG CAGTATCTGA CAATGAGTCA GAAGATGACG TTGAAAATGT AGTGGAAAAG 
GATGCGTTGA AGGAGGAATC TACCAAGGAA GCCGAAGATG TCAACCATAT AAAAAATGAG 
GACGAGACGA AGAGTCACCC CTTCTTTTAC AAAAAAATAA CTCGTCATTA CTTGGCCGAT 
GAGCCGCGAA AAGAACAGCG GGATGTACAA CTGACCGCGG CAAACTACAA CACTCGTACC 
AAGGTTTTGG TGGTAGCCTT CAGTTCCGGA GCTTTCTACC TCTACGAATT GCCCGACGTC 
AACATGATTC ATTCGCTGAG CATTTCCGAC TATCCTATTT CGGCGGCTCT CTTCAACTGC 
ACTGGTGACT GGGTCGCATT GGCCTCCCGA GAGATTGGCC AACTTCTGGT TTGGGAGTGG 
CAAAGCGAGC AATACATAAT GAAGCAGCAG GGACACAGCA GCGAAATGAC CTGCATTGCT 
TACTCCTCGG ATGGTCAGTT CATCGCCACT GGCGGCGAAG ACTCCAAGGT GAAGCTCTGG 
AACACGCAGA GCAGTTTCTG TTTTGTTACT TTCAGCGAGC ACACCAGTGG AGTTACTGGC 
ATACAGTTCA GCAGGAACAA GAAGTTCCTG GTTAGCAGTT CCCTAGACGG CACAGTGCGT 
GCTTTTGATG TCAACAGATA CCGCAACTTC AGGACTTTCA CCTCACCGAA TCCTGCTCAG 
TTCTCCTGTG TAGCTGTGGA TTACTCTGGC GAGTTAGTTG TGGCTGGCGG CCAGGACGTA 
TTCGATATCT TCCTGTGGTC AGTTAAAACA GGAAAGCTGC TGGAGATCAT CAGTGGCCAT 
GAGGGACCCG TAGTTTCCAT TGCATTCTCA CCAGTGGCTA CTTCATCAAC TTTGGTGTCT 
GGTTCTTGGG ACAAGACCGT CAAGATATGG AACTGCCTGG AGAGCAACAG CGAGCACGAA 
ACTATTGATG CTGTTTCAGA TGTTACAAAT GTTACTTTCA GTCCTAGTGG TGAAGAGATT 
GCTGTGGCCA CTCTCAGTGG TAATATAACA ATTTTCGACA TCAAATCTGC TGGCCAAGTT 
ACTACTATAG AGGGTCGCAA TGATCTGAGC GCAGGGCGCC TGGAAACCGA TATTATAACA 
GCGAGGAAAA ATGCTCAGGC AAACTATTTT TCCACCATTG AGTACTCAGC GGATGGAGAG 
TGCATACTGG CGGCTGGAAA GTCTGCAAAC ATATGCATTT ACCATGTTAG GGAGGCAATA 
CTTTTGAAGA AATTTGAGAT CACACAAAAC CACAGCTTAG ATGGCCTTAA TGACTTTATC 
AGTCGAAAAC ATTTAAGTGA ATTTGGCAAC ATGGCTCTTG TGGAAGAACG AGAAGAGCTG 
GAGGGCGGCA GGGTGGCTAT TCGATTGCCG GGCGTACAAC GGGGAGACAT GTCATCCAGG 
AGATTCCAGC AAGAGGTTAG GGTGTTCTCC GTAAAGTTCT CACCCACAGG CCAGGCATTT 
GCGGCTGCTG GCACTGAAGG ACTATGCATA TACGCCTTAG ATAAGGGTGT TGTCTTCGAT 
CCCTTTGATC TCTCGCTGGA AGTTACGCCG AAAGCGGTCC ACGAGTCCCT CAAGCAGCAG 
AATTACACCA AGGCGTTGGT CATGTCCCTC AAGCTAAACG AGCCCAATTT GATTGCCCTA 
GTGCTGGAGC GAGTGCCGTA TAAGGACGTC GAACTGGTTT GTGCGGATCT GTCGCCGGAG 
TTTGCCCAGC GACTGTTGCA ACAATTGGCC CGTCAGCTCC AGTCCACGCC ACACATAGAG 
TTCTATCTGC AATGGAGCTG CTGTCTGCTG ACCAAACACG GAAACCAAGA TGGAGTCTTT 
CAGCACACGG GCTTGCTGGC ACTGCACGAG GTTCTTTCGA GGAAATACGA AATGCTTAAC 
AAAATCTGCG ACTACAACAA GTACACGTTG AAAGTACTCC TGGATAGAGC TGATAAGTTG 
GAGCAGAAAA ACGAGGGCAA ACCCAATGCG ACGGAGGAAG ATAGTGATGA GGAAATGCTA 
CTTATCAGGT CGAAGGACGA CGAAAATGGA CAGGCGCAGT TTAGCGATGA GGAGGAGAGC 
GACAGCGGAT CCGAAGAAGA GATGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12325 ( NM_001299367.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286296.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12325), mRNA.

Price & Availability↑

CloneID	OFa12917
Clone ID Related Accession (Same CDS sequence)	NM_136774.2 , NM_001299367.1
Accession Version	NM_001299367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTT CCTACAAATT TTCCAACCTG TTGGGCACAA TATATCGTAA TGGAAACTTG 
GTGTTCACAC CGGATGGCAA TTCCGTTATC AGTCCGGTTG GCAATAGGAT AACGGTCTAC 
GATTTAAAAA ACAACAAATC GCGCACCTTG TCCCTGGAAT CGCGGTATAA CTACACGAAC 
ATAGCTCTTT CCCCGGATGG AAGTCTGCTC ATCGCAGTCA ATGAAAAGGG TGAGGCGCAG 
CTCATCAGCA TGATGTCCTG TACGGTGATA CATCGTCACA AGTTCCAGAC GGGCGTACAG 
TGCATCTGCT TCAGTCCAAA TGGAGCATTC TTTGCCGTCG CTGTGCAGAA CGTAGTGTTG 
ATATTCTGTG CACCTGGTGA GATCACAGGG GAGTACAATC CTTTCGTCCT GCGCCGAAGA 
TTCCTAGGTG GATTCGACGA TGTCACTTGG CTGGACTGGT CTTCGGACTC TCGACTTTTG 
GCAATCGGCT GTCGGGATAG CTCCACCAAA ATCGTTGCCA TCAACTATAC AAAGAACTTC 
CGCACCTATA ACCTAAGTGG CCACACAGAC GCCATTGTAG CTTGTTTTTT CGAGGCAAAC 
AGCTTGCACT TGAACACCAT AAGCCGCAAT GGTCAGCTCT GCTTGTGGGA ATGCAGCCTA 
GCTCCCTCGG ATTTAGAGGA TGCTGAAAGG CCAAAAATCG CTCCCCCTCA AAGGAAGAAG 
GAAAAGAAGG CAGTATCTGA CAATGAGTCA GAAGATGACG TTGAAAATGT AGTGGAAAAG 
GATGCGTTGA AGGAGGAATC TACCAAGGAA GCCGAAGATG TCAACCATAT AAAAAATGAG 
GACGAGACGA AGAGTCACCC CTTCTTTTAC AAAAAAATAA CTCGTCATTA CTTGGCCGAT 
GAGCCGCGAA AAGAACAGCG GGATGTACAA CTGACCGCGG CAAACTACAA CACTCGTACC 
AAGGTTTTGG TGGTAGCCTT CAGTTCCGGA GCTTTCTACC TCTACGAATT GCCCGACGTC 
AACATGATTC ATTCGCTGAG CATTTCCGAC TATCCTATTT CGGCGGCTCT CTTCAACTGC 
ACTGGTGACT GGGTCGCATT GGCCTCCCGA GAGATTGGCC AACTTCTGGT TTGGGAGTGG 
CAAAGCGAGC AATACATAAT GAAGCAGCAG GGACACAGCA GCGAAATGAC CTGCATTGCT 
TACTCCTCGG ATGGTCAGTT CATCGCCACT GGCGGCGAAG ACTCCAAGGT GAAGCTCTGG 
AACACGCAGA GCAGTTTCTG TTTTGTTACT TTCAGCGAGC ACACCAGTGG AGTTACTGGC 
ATACAGTTCA GCAGGAACAA GAAGTTCCTG GTTAGCAGTT CCCTAGACGG CACAGTGCGT 
GCTTTTGATG TCAACAGATA CCGCAACTTC AGGACTTTCA CCTCACCGAA TCCTGCTCAG 
TTCTCCTGTG TAGCTGTGGA TTACTCTGGC GAGTTAGTTG TGGCTGGCGG CCAGGACGTA 
TTCGATATCT TCCTGTGGTC AGTTAAAACA GGAAAGCTGC TGGAGATCAT CAGTGGCCAT 
GAGGGACCCG TAGTTTCCAT TGCATTCTCA CCAGTGGCTA CTTCATCAAC TTTGGTGTCT 
GGTTCTTGGG ACAAGACCGT CAAGATATGG AACTGCCTGG AGAGCAACAG CGAGCACGAA 
ACTATTGATG CTGTTTCAGA TGTTACAAAT GTTACTTTCA GTCCTAGTGG TGAAGAGATT 
GCTGTGGCCA CTCTCAGTGG TAATATAACA ATTTTCGACA TCAAATCTGC TGGCCAAGTT 
ACTACTATAG AGGGTCGCAA TGATCTGAGC GCAGGGCGCC TGGAAACCGA TATTATAACA 
GCGAGGAAAA ATGCTCAGGC AAACTATTTT TCCACCATTG AGTACTCAGC GGATGGAGAG 
TGCATACTGG CGGCTGGAAA GTCTGCAAAC ATATGCATTT ACCATGTTAG GGAGGCAATA 
CTTTTGAAGA AATTTGAGAT CACACAAAAC CACAGCTTAG ATGGCCTTAA TGACTTTATC 
AGTCGAAAAC ATTTAAGTGA ATTTGGCAAC ATGGCTCTTG TGGAAGAACG AGAAGAGCTG 
GAGGGCGGCA GGGTGGCTAT TCGATTGCCG GGCGTACAAC GGGGAGACAT GTCATCCAGG 
AGATTCCAGC AAGAGGTTAG GGTGTTCTCC GTAAAGTTCT CACCCACAGG CCAGGCATTT 
GCGGCTGCTG GCACTGAAGG ACTATGCATA TACGCCTTAG ATAAGGGTGT TGTCTTCGAT 
CCCTTTGATC TCTCGCTGGA AGTTACGCCG AAAGCGGTCC ACGAGTCCCT CAAGCAGCAG 
AATTACACCA AGGCGTTGGT CATGTCCCTC AAGCTAAACG AGCCCAATTT GATTGCCCTA 
GTGCTGGAGC GAGTGCCGTA TAAGGACGTC GAACTGGTTT GTGCGGATCT GTCGCCGGAG 
TTTGCCCAGC GACTGTTGCA ACAATTGGCC CGTCAGCTCC AGTCCACGCC ACACATAGAG 
TTCTATCTGC AATGGAGCTG CTGTCTGCTG ACCAAACACG GAAACCAAGA TGGAGTCTTT 
CAGCACACGG GCTTGCTGGC ACTGCACGAG GTTCTTTCGA GGAAATACGA AATGCTTAAC 
AAAATCTGCG ACTACAACAA GTACACGTTG AAAGTACTCC TGGATAGAGC TGATAAGTTG 
GAGCAGAAAA ACGAGGGCAA ACCCAATGCG ACGGAGGAAG ATAGTGATGA GGAAATGCTA 
CTTATCAGGT CGAAGGACGA CGAAAATGGA CAGGCGCAGT TTAGCGATGA GGAGGAGAGC 
GACAGCGGAT CCGAAGAAGA GATGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286296.1
CDS	296..3145
Translation	MKFSYKFSNLLGTIYRNGNLVFTPDGNSVISPVGNRITVYDLKNNKSRTLSLESRYNYTNIALSPDGSLLIAVNEKGEAQLISMMSCTVIHRHKFQTGVQCICFSPNGAFFAVAVQNVVLIFCAPGEITGEYNPFVLRRRFLGGFDDVTWLDWSSDSRLLAIGCRDSSTKIVAINYTKNFRTYNLSGHTDAIVACFFEANSLHLNTISRNGQLCLWECSLAPSDLEDAERPKIAPPQRKKEKKAVSDNESEDDVENVVEKDALKEESTKEAEDVNHIKNEDETKSHPFFYKKITRHYLADEPRKEQRDVQLTAANYNTRTKVLVVAFSSGAFYLYELPDVNMIHSLSISDYPISAALFNCTGDWVALASREIGQLLVWEWQSEQYIMKQQGHSSEMTCIAYSSDGQFIATGGEDSKVKLWNTQSSFCFVTFSEHTSGVTGIQFSRNKKFLVSSSLDGTVRAFDVNRYRNFRTFTSPNPAQFSCVAVDYSGELVVAGGQDVFDIFLWSVKTGKLLEIISGHEGPVVSIAFSPVATSSTLVSGSWDKTVKIWNCLESNSEHETIDAVSDVTNVTFSPSGEEIAVATLSGNITIFDIKSAGQVTTIEGRNDLSAGRLETDIITARKNAQANYFSTIEYSADGECILAAGKSANICIYHVREAILLKKFEITQNHSLDGLNDFISRKHLSEFGNMALVEEREELEGGRVAIRLPGVQRGDMSSRRFQQEVRVFSVKFSPTGQAFAAAGTEGLCIYALDKGVVFDPFDLSLEVTPKAVHESLKQQNYTKALVMSLKLNEPNLIALVLERVPYKDVELVCADLSPEFAQRLLQQLARQLQSTPHIEFYLQWSCCLLTKHGNQDGVFQHTGLLALHEVLSRKYEMLNKICDYNKYTLKVLLDRADKLEQKNEGKPNATEEDSDEEMLLIRSKDDENGQAQFSDEEESDSGSEEEMA

Target ORF information:

RefSeq Version	NM_001299367.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12325), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299367.1

ORF Insert Sequence:

ATGAAGTTTT CCTACAAATT TTCCAACCTG TTGGGCACAA TATATCGTAA TGGAAACTTG 
GTGTTCACAC CGGATGGCAA TTCCGTTATC AGTCCGGTTG GCAATAGGAT AACGGTCTAC 
GATTTAAAAA ACAACAAATC GCGCACCTTG TCCCTGGAAT CGCGGTATAA CTACACGAAC 
ATAGCTCTTT CCCCGGATGG AAGTCTGCTC ATCGCAGTCA ATGAAAAGGG TGAGGCGCAG 
CTCATCAGCA TGATGTCCTG TACGGTGATA CATCGTCACA AGTTCCAGAC GGGCGTACAG 
TGCATCTGCT TCAGTCCAAA TGGAGCATTC TTTGCCGTCG CTGTGCAGAA CGTAGTGTTG 
ATATTCTGTG CACCTGGTGA GATCACAGGG GAGTACAATC CTTTCGTCCT GCGCCGAAGA 
TTCCTAGGTG GATTCGACGA TGTCACTTGG CTGGACTGGT CTTCGGACTC TCGACTTTTG 
GCAATCGGCT GTCGGGATAG CTCCACCAAA ATCGTTGCCA TCAACTATAC AAAGAACTTC 
CGCACCTATA ACCTAAGTGG CCACACAGAC GCCATTGTAG CTTGTTTTTT CGAGGCAAAC 
AGCTTGCACT TGAACACCAT AAGCCGCAAT GGTCAGCTCT GCTTGTGGGA ATGCAGCCTA 
GCTCCCTCGG ATTTAGAGGA TGCTGAAAGG CCAAAAATCG CTCCCCCTCA AAGGAAGAAG 
GAAAAGAAGG CAGTATCTGA CAATGAGTCA GAAGATGACG TTGAAAATGT AGTGGAAAAG 
GATGCGTTGA AGGAGGAATC TACCAAGGAA GCCGAAGATG TCAACCATAT AAAAAATGAG 
GACGAGACGA AGAGTCACCC CTTCTTTTAC AAAAAAATAA CTCGTCATTA CTTGGCCGAT 
GAGCCGCGAA AAGAACAGCG GGATGTACAA CTGACCGCGG CAAACTACAA CACTCGTACC 
AAGGTTTTGG TGGTAGCCTT CAGTTCCGGA GCTTTCTACC TCTACGAATT GCCCGACGTC 
AACATGATTC ATTCGCTGAG CATTTCCGAC TATCCTATTT CGGCGGCTCT CTTCAACTGC 
ACTGGTGACT GGGTCGCATT GGCCTCCCGA GAGATTGGCC AACTTCTGGT TTGGGAGTGG 
CAAAGCGAGC AATACATAAT GAAGCAGCAG GGACACAGCA GCGAAATGAC CTGCATTGCT 
TACTCCTCGG ATGGTCAGTT CATCGCCACT GGCGGCGAAG ACTCCAAGGT GAAGCTCTGG 
AACACGCAGA GCAGTTTCTG TTTTGTTACT TTCAGCGAGC ACACCAGTGG AGTTACTGGC 
ATACAGTTCA GCAGGAACAA GAAGTTCCTG GTTAGCAGTT CCCTAGACGG CACAGTGCGT 
GCTTTTGATG TCAACAGATA CCGCAACTTC AGGACTTTCA CCTCACCGAA TCCTGCTCAG 
TTCTCCTGTG TAGCTGTGGA TTACTCTGGC GAGTTAGTTG TGGCTGGCGG CCAGGACGTA 
TTCGATATCT TCCTGTGGTC AGTTAAAACA GGAAAGCTGC TGGAGATCAT CAGTGGCCAT 
GAGGGACCCG TAGTTTCCAT TGCATTCTCA CCAGTGGCTA CTTCATCAAC TTTGGTGTCT 
GGTTCTTGGG ACAAGACCGT CAAGATATGG AACTGCCTGG AGAGCAACAG CGAGCACGAA 
ACTATTGATG CTGTTTCAGA TGTTACAAAT GTTACTTTCA GTCCTAGTGG TGAAGAGATT 
GCTGTGGCCA CTCTCAGTGG TAATATAACA ATTTTCGACA TCAAATCTGC TGGCCAAGTT 
ACTACTATAG AGGGTCGCAA TGATCTGAGC GCAGGGCGCC TGGAAACCGA TATTATAACA 
GCGAGGAAAA ATGCTCAGGC AAACTATTTT TCCACCATTG AGTACTCAGC GGATGGAGAG 
TGCATACTGG CGGCTGGAAA GTCTGCAAAC ATATGCATTT ACCATGTTAG GGAGGCAATA 
CTTTTGAAGA AATTTGAGAT CACACAAAAC CACAGCTTAG ATGGCCTTAA TGACTTTATC 
AGTCGAAAAC ATTTAAGTGA ATTTGGCAAC ATGGCTCTTG TGGAAGAACG AGAAGAGCTG 
GAGGGCGGCA GGGTGGCTAT TCGATTGCCG GGCGTACAAC GGGGAGACAT GTCATCCAGG 
AGATTCCAGC AAGAGGTTAG GGTGTTCTCC GTAAAGTTCT CACCCACAGG CCAGGCATTT 
GCGGCTGCTG GCACTGAAGG ACTATGCATA TACGCCTTAG ATAAGGGTGT TGTCTTCGAT 
CCCTTTGATC TCTCGCTGGA AGTTACGCCG AAAGCGGTCC ACGAGTCCCT CAAGCAGCAG 
AATTACACCA AGGCGTTGGT CATGTCCCTC AAGCTAAACG AGCCCAATTT GATTGCCCTA 
GTGCTGGAGC GAGTGCCGTA TAAGGACGTC GAACTGGTTT GTGCGGATCT GTCGCCGGAG 
TTTGCCCAGC GACTGTTGCA ACAATTGGCC CGTCAGCTCC AGTCCACGCC ACACATAGAG 
TTCTATCTGC AATGGAGCTG CTGTCTGCTG ACCAAACACG GAAACCAAGA TGGAGTCTTT 
CAGCACACGG GCTTGCTGGC ACTGCACGAG GTTCTTTCGA GGAAATACGA AATGCTTAAC 
AAAATCTGCG ACTACAACAA GTACACGTTG AAAGTACTCC TGGATAGAGC TGATAAGTTG 
GAGCAGAAAA ACGAGGGCAA ACCCAATGCG ACGGAGGAAG ATAGTGATGA GGAAATGCTA 
CTTATCAGGT CGAAGGACGA CGAAAATGGA CAGGCGCAGT TTAGCGATGA GGAGGAGAGC 
GACAGCGGAT CCGAAGAAGA GATGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12325 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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