trsn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	trsn
Entrez Gene ID	36110
Full Name	translin
Synonyms	CG11761,Dmel\CG11761,TB-RBP
General protein information	Preferred Names translin Names CG11761 gene product from transcript CG11761-RA CG11761-PA testis-brain RNA binding protein trsn-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47A11-47A11

mRNA and Protein(s)

mRNA	Protein	Name
NM_136747.4	NP_610591.1	translin

Pathways from BioSystems

Homologies

Homo sapiens (human)	TSN	NP_004613.1
	TSN	XP_001085908.1
Bos taurus (cattle)	TSN	NP_001068890.1
Gallus gallus (chicken)	TSN	NP_990404.1
Neurospora crassa	NCU06664	XP_960911.1
Pan troglodytes (chimpanzee)	TSN	XP_515770.1
Mus musculus (house mouse)	Tsn	NP_035780.1
Arabidopsis thaliana (thale cress)	AT2G37020	NP_565857.2
Danio rerio (zebrafish)	tsn	NP_001020623.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005538	XP_315537.4
Magnaporthe oryzae (rice blast fungus)	MGG_05995	XP_003711841.1
Xenopus tropicalis (tropical clawed frog)	mllt4	NP_001007517.1
Canis lupus familiaris (dog)	TSN	XP_003639748.1
Rattus norvegicus (Norway rat)	Tsn	NP_068530.1
Schizosaccharomyces pombe (fission yeast)	tsn1	NP_594557.2
Oryza sativa (rice)	Os10g0443200	NP_001064703.1
Drosophila melanogaster (fruit fly)	trsn	NP_610591.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study describes the identification of translin (trsn), a highly conserved RNA/DNA binding protein, as essential for starvation-induced sleep suppression. Strikingly, trsn does not appear to regulate energy stores, free glucose levels, or feeding behavior suggesting the sleep phenotype of trsn mutant flies is not a consequence of general metabolic dysfunction or blunted response to starvation.
Title: translin Is Required for Metabolic Regulation of Sleep.

These studies establish that Trax adopts the translin fold, possesses catalytic centers essential for C3PO's endoRNase activity and interacts extensively with translin to form an octameric assembly.
Title: Multimeric assembly and biochemical characterization of the Trax-translin endonuclease complex.

an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax was purified; C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products
Title: C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.

Results suggest that the oligomeric status of translin critically influences the DNA binding properties of translin proteins.
Title: Crystal structures of Drosophila mutant translin and characterization of translin variants reveal the structural plasticity of translin proteins.

Drosophila translin may have roles in neuronal development and behavior analogous to that of mouse translin.
Title: Expression pattern of Drosophila translin and behavioral analyses of the mutant.

The following trsn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trsn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12889	NM_136747.4 Latest version!	Drosophila melanogaster translin (trsn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trsn ( NM_136747.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610591.1 Drosophila melanogaster translin (trsn), mRNA.

Price & Availability↑

CloneID	OFa12889
Clone ID Related Accession (Same CDS sequence)	NM_136747.4
Accession Version	NM_136747.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136747.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAACT TCGTGAACTT GGACATCTTC TCCAACTACC AAAAGTACAT AGACAATGAA 
CAGGAAGTCA GGGAGAACAT CCGCATTGTG GTGCGCGAAA TCGAGCATTT GTCAAAGGAA 
GCGCAGATTA AACTGCAGAT TATTCATAGC GATTTGAGCC AGATTAGTGC CGCCTGCGGC 
TTAGCCCGCA AACAGGTTGA GCTGTGTGCC CAAAAGTACC AGAAATTGGC CGAACTGGTG 
CCAGCTGGGC AGTACTACAG ATACTCCGAT CACTGGACCT TCATTACGCA GCGTTTGATC 
TTCATCATTG CCTTGGTTAT TTACCTGGAG GCGGGCTTCT TGGTCACCCG CGAAACAGTG 
GCCGAAATGC TGGGATTGAA GATCAGCCAG TCTGAGGGCT TCCATCTGGA TGTGGAGGAC 
TATCTACTGG GCATACTGCA GTTGGCGTCG GAGCTCTCCC GATTCGCTAC CAATTCCGTC 
ACCATGGGCG ACTACGAGCG TCCCCTGAAT ATCTCCCATT TTATTGGTGA CCTGAACACG 
GGCTTCCGTC TGCTGAACCT GAAGAACGAT GGCTTGCGAA AGCGCTTCGA TGCCTTAAAG 
TATGATGTCA AGAAGATCGA GGAGGTCGTC TACGATGTCA GCATACGCGG TCTGTCCAGC 
AAGGAAAAGG ACCAGCAGGA GGAGCCGGCT GTTCCTGCAA CCGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610591.1
CDS	74..781
Translation	MSNFVNLDIFSNYQKYIDNEQEVRENIRIVVREIEHLSKEAQIKLQIIHSDLSQISAACGLARKQVELCAQKYQKLAELVPAGQYYRYSDHWTFITQRLIFIIALVIYLEAGFLVTRETVAEMLGLKISQSEGFHLDVEDYLLGILQLASELSRFATNSVTMGDYERPLNISHFIGDLNTGFRLLNLKNDGLRKRFDALKYDVKKIEEVVYDVSIRGLSSKEKDQQEEPAVPATE

Target ORF information:

RefSeq Version	NM_136747.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translin (trsn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136747.4

ORF Insert Sequence:

ATGTCGAACT TCGTGAACTT GGACATCTTC TCCAACTACC AAAAGTACAT AGACAATGAA 
CAGGAAGTCA GGGAGAACAT CCGCATTGTG GTGCGCGAAA TCGAGCATTT GTCAAAGGAA 
GCGCAGATTA AACTGCAGAT TATTCATAGC GATTTGAGCC AGATTAGTGC CGCCTGCGGC 
TTAGCCCGCA AACAGGTTGA GCTGTGTGCC CAAAAGTACC AGAAATTGGC CGAACTGGTG 
CCAGCTGGGC AGTACTACAG ATACTCCGAT CACTGGACCT TCATTACGCA GCGTTTGATC 
TTCATCATTG CCTTGGTTAT TTACCTGGAG GCGGGCTTCT TGGTCACCCG CGAAACAGTG 
GCCGAAATGC TGGGATTGAA GATCAGCCAG TCTGAGGGCT TCCATCTGGA TGTGGAGGAC 
TATCTACTGG GCATACTGCA GTTGGCGTCG GAGCTCTCCC GATTCGCTAC CAATTCCGTC 
ACCATGGGCG ACTACGAGCG TCCCCTGAAT ATCTCCCATT TTATTGGTGA CCTGAACACG 
GGCTTCCGTC TGCTGAACCT GAAGAACGAT GGCTTGCGAA AGCGCTTCGA TGCCTTAAAG 
TATGATGTCA AGAAGATCGA GGAGGTCGTC TACGATGTCA GCATACGCGG TCTGTCCAGC 
AAGGAAAAGG ACCAGCAGGA GGAGCCGGCT GTTCCTGCAA CCGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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trsn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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