Syb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Syb
Entrez Gene ID	36080
Full Name	Synaptobrevin
Synonyms	21.15,CG12210,Dmel\CG12210,END1-2,J,SYB,VAMP,VAMP2,Vamp,c-Syb,dVAMP,l(2)46CFq,l(2)46El,syb,synaptobrevin
General protein information	Preferred Names Synaptobrevin Names CG12210 gene product from transcript CG12210-RA CG12210-PA CG12210-PB CG12210-PC CG12210-PD CG12210-PE Syb-PA Syb-PB Syb-PC Syb-PD Syb-PE VAMP3 Vesicle-associated membrane protein complementation group J synaptobrevin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46F8-46F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_165756.3	NP_724906.1	synaptobrevin, isoform B
NM_001144161.2	NP_001137633.1	synaptobrevin, isoform C
NM_078956.4	NP_523680.1	synaptobrevin, isoform A
NM_001144162.2	NP_001137634.1	synaptobrevin, isoform D
NM_001299349.1	NP_001286278.1	synaptobrevin, isoform E

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VAMP1	XP_003313489.1
	VAMP1	XP_002798503.1
Gallus gallus (chicken)	VAMP1	NP_001034575.1
Danio rerio (zebrafish)	zgc:92912	NP_001002532.1
Mus musculus (house mouse)	Vamp1	NP_033522.1
Danio rerio (zebrafish)	vamp1	NP_998563.1
Drosophila melanogaster (fruit fly)	Syb	NP_724906.1
Homo sapiens (human)	VAMP1	NP_055046.1
Canis lupus familiaris (dog)	VAMP1	XP_005637307.1
Caenorhabditis elegans (roundworm)	snb-1	NP_504688.1
Xenopus tropicalis (tropical clawed frog)	vamp1	NP_001106401.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007721	XP_001687848.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Interaction of the Complexin Accessory Helix with Synaptobrevin Regulates Spontaneous Fusion
Title: Interaction of the Complexin Accessory Helix with Synaptobrevin Regulates Spontaneous Fusion.

Expression of syb can restore synaptic transmission to n-syb mutants as assayed both by electroretinogram and recordings of excitatory junctional currents at the neuromuscular junction.
Title: Members of the synaptobrevin/vesicle-associated membrane protein (VAMP) family in Drosophila are functionally interchangeable in vivo for neurotransmitter release and cell viability.

The following Syb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Syb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09489	NM_001299349.1 Latest version!	Drosophila melanogaster synaptobrevin, transcript variant E (Syb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa09489	NM_078956.4 Latest version!	Drosophila melanogaster synaptobrevin, transcript variant A (Syb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa09490	NM_001144162.2 Latest version!	Drosophila melanogaster synaptobrevin, transcript variant D (Syb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa09491	NM_001144161.2 Latest version!	Drosophila melanogaster synaptobrevin, transcript variant C (Syb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa09492	NM_165756.3 Latest version!	Drosophila melanogaster synaptobrevin, transcript variant B (Syb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Syb ( NM_078956.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523680.1 Drosophila melanogaster synaptobrevin, transcript variant A (Syb), mRNA.

Price & Availability↑

CloneID	OFa09489
Clone ID Related Accession (Same CDS sequence)	NM_078956.4 , NM_001299349.1
Accession Version	NM_078956.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptobrevin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078956.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGAGAA CAACAATGCA 
GCCCAGAAGA AGCTGCAGCA GACCCAAGCC AAGGTGGACG AGGTGGTCGG GATTATGCGT 
GTGAACGTGG AGAAGGTCCT GGAGCGGGAC CAGAAGCTAT CGGAACTGGG CGAGCGTGCG 
GATCAGCTGG AGCAGGGAGC ATCCCAGTTC GAGCAGCAGG CCGGCAAGCT GAAGCGCAAG 
CAATGGTGGG CCAACATGAA GATGATGATC ATTCTGGGCG TGATAGCCGT TGTGCTGCTC 
ATCATCGTTC TGGTGTCCGT TTGGCCGTCT AGTAGTGACA GCGGCAGTGG TGGAGGAAAC 
AAGGCCATCA CCCAAGCACC GCCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523680.1
CDS	147..536
Translation	MENNEAPSPSGSNNNENNNAAQKKLQQTQAKVDEVVGIMRVNVEKVLERDQKLSELGERADQLEQGASQFEQQAGKLKRKQWWANMKMMIILGVIAVVLLIIVLVSVWPSSSDSGSGGGNKAITQAPPH

Target ORF information:

RefSeq Version	NM_078956.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptobrevin, transcript variant A (Syb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078956.4

ORF Insert Sequence:

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGAGAA CAACAATGCA 
GCCCAGAAGA AGCTGCAGCA GACCCAAGCC AAGGTGGACG AGGTGGTCGG GATTATGCGT 
GTGAACGTGG AGAAGGTCCT GGAGCGGGAC CAGAAGCTAT CGGAACTGGG CGAGCGTGCG 
GATCAGCTGG AGCAGGGAGC ATCCCAGTTC GAGCAGCAGG CCGGCAAGCT GAAGCGCAAG 
CAATGGTGGG CCAACATGAA GATGATGATC ATTCTGGGCG TGATAGCCGT TGTGCTGCTC 
ATCATCGTTC TGGTGTCCGT TTGGCCGTCT AGTAGTGACA GCGGCAGTGG TGGAGGAAAC 
AAGGCCATCA CCCAAGCACC GCCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syb ( NM_001299349.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286278.1 Drosophila melanogaster synaptobrevin, transcript variant E (Syb), mRNA.

Price & Availability↑

CloneID	OFa09489
Clone ID Related Accession (Same CDS sequence)	NM_078956.4 , NM_001299349.1
Accession Version	NM_001299349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptobrevin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGAGAA CAACAATGCA 
GCCCAGAAGA AGCTGCAGCA GACCCAAGCC AAGGTGGACG AGGTGGTCGG GATTATGCGT 
GTGAACGTGG AGAAGGTCCT GGAGCGGGAC CAGAAGCTAT CGGAACTGGG CGAGCGTGCG 
GATCAGCTGG AGCAGGGAGC ATCCCAGTTC GAGCAGCAGG CCGGCAAGCT GAAGCGCAAG 
CAATGGTGGG CCAACATGAA GATGATGATC ATTCTGGGCG TGATAGCCGT TGTGCTGCTC 
ATCATCGTTC TGGTGTCCGT TTGGCCGTCT AGTAGTGACA GCGGCAGTGG TGGAGGAAAC 
AAGGCCATCA CCCAAGCACC GCCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286278.1
CDS	109..498
Translation	MENNEAPSPSGSNNNENNNAAQKKLQQTQAKVDEVVGIMRVNVEKVLERDQKLSELGERADQLEQGASQFEQQAGKLKRKQWWANMKMMIILGVIAVVLLIIVLVSVWPSSSDSGSGGGNKAITQAPPH

Target ORF information:

RefSeq Version	NM_001299349.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptobrevin, transcript variant E (Syb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299349.1

ORF Insert Sequence:

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGAGAA CAACAATGCA 
GCCCAGAAGA AGCTGCAGCA GACCCAAGCC AAGGTGGACG AGGTGGTCGG GATTATGCGT 
GTGAACGTGG AGAAGGTCCT GGAGCGGGAC CAGAAGCTAT CGGAACTGGG CGAGCGTGCG 
GATCAGCTGG AGCAGGGAGC ATCCCAGTTC GAGCAGCAGG CCGGCAAGCT GAAGCGCAAG 
CAATGGTGGG CCAACATGAA GATGATGATC ATTCTGGGCG TGATAGCCGT TGTGCTGCTC 
ATCATCGTTC TGGTGTCCGT TTGGCCGTCT AGTAGTGACA GCGGCAGTGG TGGAGGAAAC 
AAGGCCATCA CCCAAGCACC GCCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syb ( NM_001144162.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137634.1 Drosophila melanogaster synaptobrevin, transcript variant D (Syb), mRNA.

Price & Availability↑

CloneID	OFa09490
Clone ID Related Accession (Same CDS sequence)	NM_001144162.2
Accession Version	NM_001144162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptobrevin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144162.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGAGAA CAACAATGCA 
GCCCAGAAGA AGCTGCAGCA GACCCAAGCC AAGGTGGACG AGGTGGTCGG GATTATGCGT 
GTGAACGTGG AGAAGGTCCT GGAGCGGGAC CAGAAGCTAT CGGAACTGGG CGAGCGTGCG 
GATCAGCTGG AGCAGGGAGC ATCCCAGTTC GAGCAGCAGG CCGGCAAGCT GAAGCGCAAG 
CAATGGTGGG CCAACATGAA GATGATGATC ATTCTGGGCG TGATAGCCGT TGTGCTGCTC 
ATCATCGTTC TGGTGTCGCT TTTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137634.1
CDS	147..476
Translation	MENNEAPSPSGSNNNENNNAAQKKLQQTQAKVDEVVGIMRVNVEKVLERDQKLSELGERADQLEQGASQFEQQAGKLKRKQWWANMKMMIILGVIAVVLLIIVLVSLFN

Target ORF information:

RefSeq Version	NM_001144162.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptobrevin, transcript variant D (Syb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144162.2

ORF Insert Sequence:

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGAGAA CAACAATGCA 
GCCCAGAAGA AGCTGCAGCA GACCCAAGCC AAGGTGGACG AGGTGGTCGG GATTATGCGT 
GTGAACGTGG AGAAGGTCCT GGAGCGGGAC CAGAAGCTAT CGGAACTGGG CGAGCGTGCG 
GATCAGCTGG AGCAGGGAGC ATCCCAGTTC GAGCAGCAGG CCGGCAAGCT GAAGCGCAAG 
CAATGGTGGG CCAACATGAA GATGATGATC ATTCTGGGCG TGATAGCCGT TGTGCTGCTC 
ATCATCGTTC TGGTGTCGCT TTTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syb ( NM_001144161.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137633.1 Drosophila melanogaster synaptobrevin, transcript variant C (Syb), mRNA.

Price & Availability↑

CloneID	OFa09491
Clone ID Related Accession (Same CDS sequence)	NM_001144161.2
Accession Version	NM_001144161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptobrevin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144161.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGATTT TCCCATACTT 
CCGCCACCGC CGAACGCGAA TGACAACTAC AATCAATTCG GTGACCATCA AATCAGGAAC 
AACAATGCAG CCCAGAAGAA GCTGCAGCAG ACCCAAGCCA AGGTGGACGA GGTGGTCGGG 
ATTATGCGTG TGAACGTGGA GAAGGTCCTG GAGCGGGACC AGAAGCTATC GGAACTGGGC 
GAGCGTGCGG ATCAGCTGGA GCAGGGAGCA TCCCAGTTCG AGCAGCAGGC CGGCAAGCTG 
AAGCGCAAGC AATGGTGGGC CAACATGAAG ATGATGATCA TTCTGGGCGT GATAGCCGTT 
GTGCTGCTCA TCATCGTTCT GGTGTCCGTT TGGCCGTCTA GTAGTGACAG CGGCAGTGGT 
GGAGGAAACA AGGCCATCAC CCAAGCACCG CCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137633.1
CDS	147..605
Translation	MENNEAPSPSGSNNNDFPILPPPPNANDNYNQFGDHQIRNNNAAQKKLQQTQAKVDEVVGIMRVNVEKVLERDQKLSELGERADQLEQGASQFEQQAGKLKRKQWWANMKMMIILGVIAVVLLIIVLVSVWPSSSDSGSGGGNKAITQAPPH

Target ORF information:

RefSeq Version	NM_001144161.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptobrevin, transcript variant C (Syb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144161.2

ORF Insert Sequence:

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGATTT TCCCATACTT 
CCGCCACCGC CGAACGCGAA TGACAACTAC AATCAATTCG GTGACCATCA AATCAGGAAC 
AACAATGCAG CCCAGAAGAA GCTGCAGCAG ACCCAAGCCA AGGTGGACGA GGTGGTCGGG 
ATTATGCGTG TGAACGTGGA GAAGGTCCTG GAGCGGGACC AGAAGCTATC GGAACTGGGC 
GAGCGTGCGG ATCAGCTGGA GCAGGGAGCA TCCCAGTTCG AGCAGCAGGC CGGCAAGCTG 
AAGCGCAAGC AATGGTGGGC CAACATGAAG ATGATGATCA TTCTGGGCGT GATAGCCGTT 
GTGCTGCTCA TCATCGTTCT GGTGTCCGTT TGGCCGTCTA GTAGTGACAG CGGCAGTGGT 
GGAGGAAACA AGGCCATCAC CCAAGCACCG CCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syb ( NM_165756.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724906.1 Drosophila melanogaster synaptobrevin, transcript variant B (Syb), mRNA.

Price & Availability↑

CloneID	OFa09492
Clone ID Related Accession (Same CDS sequence)	NM_165756.3
Accession Version	NM_165756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	synaptobrevin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165756.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGATTT TCCCATACTT 
CCGCCACCGC CGAACGCGAA TGACAACTAC AATCAATTCG GTGACCATCA AATCAGGAAC 
AACAATGCAG CCCAGAAGAA GCTGCAGCAG ACCCAAGCCA AGGTGGACGA GGTGGTCGGG 
ATTATGCGTG TGAACGTGGA GAAGGTCCTG GAGCGGGACC AGAAGCTATC GGAACTGGGC 
GAGCGTGCGG ATCAGCTGGA GCAGGGAGCA TCCCAGTTCG AGCAGCAGGC CGGCAAGCTG 
AAGCGCAAGC AATGGTGGGC CAACATGAAG ATGATGATCA TTCTGGGCGT GATAGCCGTT 
GTGCTGCTCA TCATCGTTCT GGTGTCGCTT TTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724906.1
CDS	147..545
Translation	MENNEAPSPSGSNNNDFPILPPPPNANDNYNQFGDHQIRNNNAAQKKLQQTQAKVDEVVGIMRVNVEKVLERDQKLSELGERADQLEQGASQFEQQAGKLKRKQWWANMKMMIILGVIAVVLLIIVLVSLFN

Target ORF information:

RefSeq Version	NM_165756.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster synaptobrevin, transcript variant B (Syb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165756.3

ORF Insert Sequence:

ATGGAGAACA ACGAAGCCCC CTCCCCCTCG GGATCCAACA ACAACGATTT TCCCATACTT 
CCGCCACCGC CGAACGCGAA TGACAACTAC AATCAATTCG GTGACCATCA AATCAGGAAC 
AACAATGCAG CCCAGAAGAA GCTGCAGCAG ACCCAAGCCA AGGTGGACGA GGTGGTCGGG 
ATTATGCGTG TGAACGTGGA GAAGGTCCTG GAGCGGGACC AGAAGCTATC GGAACTGGGC 
GAGCGTGCGG ATCAGCTGGA GCAGGGAGCA TCCCAGTTCG AGCAGCAGGC CGGCAAGCTG 
AAGCGCAAGC AATGGTGGGC CAACATGAAG ATGATGATCA TTCTGGGCGT GATAGCCGTT 
GTGCTGCTCA TCATCGTTCT GGTGTCGCTT TTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Syb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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