Pfk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfk
Entrez Gene ID	36060
Full Name	Phosphofructokinase
Synonyms	BcDNA.GH12192,BcDNA:GH12192,CG4001,Dmel\CG4001,PFK,PFK1,l(2)06339,pfk
General protein information	Preferred Names Phosphofructokinase Names CG4001 gene product from transcript CG4001-RE 6-phosphofructokinase CG4001-PA CG4001-PB CG4001-PC CG4001-PD CG4001-PE Pfk-PA Pfk-PB Pfk-PC Pfk-PD Pfk-PE phosphofructokinase phosphofructokinase 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46E8-46F1\|2-85.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259305.1	NP_001246234.1	phosphofructokinase, isoform D
NM_078952.3	NP_523676.1	phosphofructokinase, isoform A
NM_165746.3	NP_724890.1	phosphofructokinase, isoform B
NM_165747.4	NP_724891.2	phosphofructokinase, isoform C
NM_001273918.1	NP_001260847.1	phosphofructokinase, isoform E

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pfk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09466	NM_078952.3 Latest version!	Drosophila melanogaster phosphofructokinase, transcript variant A (Pfk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09467	NM_165746.3 Latest version!	Drosophila melanogaster phosphofructokinase, transcript variant B (Pfk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09468	NM_165747.4 Latest version!	Drosophila melanogaster phosphofructokinase, transcript variant C (Pfk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa12875	NM_001273918.1 Latest version!	Drosophila melanogaster phosphofructokinase, transcript variant E (Pfk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12878	NM_001259305.1 Latest version!	Drosophila melanogaster phosphofructokinase, transcript variant D (Pfk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfk ( NM_078952.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523676.1 Drosophila melanogaster phosphofructokinase, transcript variant A (Pfk), mRNA.

Price & Availability↑

CloneID	OFa09466
Clone ID Related Accession (Same CDS sequence)	NM_078952.3
Accession Version	NM_078952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphofructokinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_078952.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCAA TAAAATTTCG AGTATTTACC AAATTAAAAC CTATATTTTT GGAAATCAAT 
GGACGGATAC CCATTTGCCG CCATTTTCAT GGTCCTACAA CATTTCGCTT AGAAATCTCA 
AACAAAACAC CTCCGATTAG GCAAAAGCTC ACATTTCCAA ACATCGGCAT CCAATGCACA 
CGAAGTCATC ATTTGTGTTG TCCTCGAGAT ATTTCAGGAA ATACTCTTTT AAGTGTAAAA 
TTTAATTGCA AACGACATTG TATAAAGTTG CGCAGTGATT CTGGTGATCA AAAGAATGAT 
AGTCCTGGTG AAAAAAACAT ACAAAAAGAC AAGTCTGCGC AACGATGTGG AAAACCAATA 
AACAATCTGC ACAACGGATT TCTTAATGCT GTGAACTATT CTGAAAAGAA TGCGGTGAAA 
AAGAAAAAGA GTGCACCAAA AAGGAAATGT GGCAAATCGG TGGATGAGCT GCGAAAGTGT 
TTAAGAACCA TGCAGGATGT TATTGATTTT GTACATCCAG TCAAGCCTTT TAAGGACAAA 
GGACTGGCGG TCTTCACCAG CGGCGGGGAT TCCCAGGGCA TGAATGCCGC CGTGCGAGCC 
TGTGTCCGTA TGGCCATCTA TTTGGGCTGC AAGGTCTACT TCATTCGTGA AGGCTACCAG 
GGCATGGTGG ATGGTGGCGA CTGCATCCAG GAAGCCAACT GGGCCTCAGT CTCATCCATT 
ATCCATCGTG GTGGCACCAT CATTGGCTCC GCCCGTTGCC AGGACTTCCG TGAGCGTCAG 
GGTCGCTTGA AAGCCGCTAA CAACCTGATT CAGCGAGGAA TCACCAATCT GGTGGTCATT 
GGAGGCGATG GCTCCCTCAC GGGCGCCAAT CTGTTCCGTC AGGAGTGGTC CAGCCTGTTG 
GACGAGCTGG TCAAGAACAA GACCATTACC ACCGAGCAGC AGGAGAAGTT CAATGTCCTG 
CACATCGTTG GATTGGTTGG CTCCATCGAC AACGATTTCT GTGGCACTGA CATGACCATC 
GGCACGGACA CGGCACTACA TCGCATCATC GAGGCAATCG ATGCCATCTC CAGTACAGCC 
TACTCCCATC AGCGCACCTT CATCATGGAG GTCATGGGTC GTCATTGCGG CTATTTAGCT 
CTTGTGGGTG GACTGGCGTG CGAGGCGGAT TTCATATTCA TTCCCGAAAT GCCGCCCAAG 
GTCGATTGGC CAGACAGGCT CTGCTCTCAG CTGGCCCAGG AGCGATCTGC TGGCCAGCGT 
CTGAACATCG TGATTGTGGC CGAGGGAGCC ATGGATCGCG AGGGTCATCC CATCACAGCC 
GAGGATGTAA AGAAGGTGAT CGACGAGCGT CTGAAGCACG ATGCCCGCAT CACTGTCTTG 
GGTCACGTGC AGCGCGGTGG CAATCCCAGT GCCTTCGATC GTATTTTGGC TTGCCGCATG 
GGAGCTGAGG CCACTTTGGC CCTGATGGAG GCCACCAAGG ACTCGGTGCC AGTGGTCATC 
TCTCTGGACG GCAACCAGGC GGTTCGCGTG CCGCTGATGG AGTGCGTAGA GCGCACCCAG 
GCTGTGGCCA AGGCCATGGC GGAGAAACGC TGGGCGGATG CCGTCAAGCT GCGCGGACGC 
TCCTTCGAGC GGAATCTGGA GACCTACAAG ATGCTGACGC GCTTGAAGCC GCCCAAGGAG 
AACTTCGACG CCGATGGCAA GGGAATCGAA GGATACCGCC TAGCTGTGAT GCATATTGGT 
GCTCCGGCTT GCGGTATGAA TGCCGCTGTG CGCAGCTTCG TGCGCAATGC CATCTACCGC 
GGTGATGTGG TTTATGGAAT CAACGACGGT GTCGAGGGTC TGATTGCCGG AAATGTCCGC 
GAGCTGGGCT GGTCGGATGT CTCAGGATGG GTTGGTCAGG GTGGCGCCTA CTTGGGTACC 
AAACGCACAC TGCCTGAGGG CAAGTTCAAG GAGATCGCCG CTCGTCTCAA GGAGTTTAAG 
ATCCAGGGTC TCCTGATCAT TGGTGGCTTT GAGAGTTACC ATGCCGCCGG ACAGATCGCC 
GATCAGCGGG ACAACTACCC ACAGTTCTGC ATCCCCATTG TGGTTATTCC ATCGACGATT 
TCGAACAATG TGCCCGGCAC AGAATTTTCT CTGGGATGCG ACACCGGTTT AAATGAGATT 
ACGGAGATTT GCGACCGTAT CCGTCAGTCG GCACAGGGAA CCAAGCGCCG AGTGTTCGTC 
ATTGAGACGA TGGGTGGCTA CTGTGGCTAT TTGGCCACCT TGGCCGGCTT GGCCGGCGGA 
GCTGATGCCG CCTACATCTA CGAGGAGAAG TTCTCCATCA AAGACCTGCA GCAGGATGTC 
TACCACATGG CCTCCAAGAT GGCCGAGGGC GTCTCCCGCG GTCTAATCCT GCGAAACGAG 
AAGGCTAGCG AGAACTACAG CACGGACTTC ATTTACCGCC TGTACTCGGA GGAGGGCAAG 
GGCCTCTTCA CCTGCCGTAT GAACATCCTG GGTCACATGC AGCAGGGCGG CTCACCCACT 
CCCTTCGACC GCAACATGGG CACCAAAATG GCCGCCAAGT GTGTCGACTG GCTGGCCGCC 
CAGATCAAGG CGAACATCGA CGCCAACGGC GTAGTCAACT GCAAGTCCCC GGACACCGCC 
ACGCTGCTGG GAATCGTGTC GCGGCAGTAC CGCTTCTCAC CGCTGGTCGA CCTCATTGCA 
GAGACGAACT TCGATCAACG CATCCCGAAG AAGCAGTGGT GGCTGCGCCT GCGTCCCCTG 
CTGCGCATCC TGGCCAAGCA CGATTCCGCC TACGAGGAGG AGGGTATGTA CATCACCGTC 
GAGGAGGAGT GTGACACTGA CGCCGTCGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523676.1
CDS	103..2955
Translation	MHSIKFRVFTKLKPIFLEINGRIPICRHFHGPTTFRLEISNKTPPIRQKLTFPNIGIQCTRSHHLCCPRDISGNTLLSVKFNCKRHCIKLRSDSGDQKNDSPGEKNIQKDKSAQRCGKPINNLHNGFLNAVNYSEKNAVKKKKSAPKRKCGKSVDELRKCLRTMQDVIDFVHPVKPFKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA

Target ORF information:

RefSeq Version	NM_078952.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphofructokinase, transcript variant A (Pfk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078952.3

ORF Insert Sequence:

ATGCATTCAA TAAAATTTCG AGTATTTACC AAATTAAAAC CTATATTTTT GGAAATCAAT 
GGACGGATAC CCATTTGCCG CCATTTTCAT GGTCCTACAA CATTTCGCTT AGAAATCTCA 
AACAAAACAC CTCCGATTAG GCAAAAGCTC ACATTTCCAA ACATCGGCAT CCAATGCACA 
CGAAGTCATC ATTTGTGTTG TCCTCGAGAT ATTTCAGGAA ATACTCTTTT AAGTGTAAAA 
TTTAATTGCA AACGACATTG TATAAAGTTG CGCAGTGATT CTGGTGATCA AAAGAATGAT 
AGTCCTGGTG AAAAAAACAT ACAAAAAGAC AAGTCTGCGC AACGATGTGG AAAACCAATA 
AACAATCTGC ACAACGGATT TCTTAATGCT GTGAACTATT CTGAAAAGAA TGCGGTGAAA 
AAGAAAAAGA GTGCACCAAA AAGGAAATGT GGCAAATCGG TGGATGAGCT GCGAAAGTGT 
TTAAGAACCA TGCAGGATGT TATTGATTTT GTACATCCAG TCAAGCCTTT TAAGGACAAA 
GGACTGGCGG TCTTCACCAG CGGCGGGGAT TCCCAGGGCA TGAATGCCGC CGTGCGAGCC 
TGTGTCCGTA TGGCCATCTA TTTGGGCTGC AAGGTCTACT TCATTCGTGA AGGCTACCAG 
GGCATGGTGG ATGGTGGCGA CTGCATCCAG GAAGCCAACT GGGCCTCAGT CTCATCCATT 
ATCCATCGTG GTGGCACCAT CATTGGCTCC GCCCGTTGCC AGGACTTCCG TGAGCGTCAG 
GGTCGCTTGA AAGCCGCTAA CAACCTGATT CAGCGAGGAA TCACCAATCT GGTGGTCATT 
GGAGGCGATG GCTCCCTCAC GGGCGCCAAT CTGTTCCGTC AGGAGTGGTC CAGCCTGTTG 
GACGAGCTGG TCAAGAACAA GACCATTACC ACCGAGCAGC AGGAGAAGTT CAATGTCCTG 
CACATCGTTG GATTGGTTGG CTCCATCGAC AACGATTTCT GTGGCACTGA CATGACCATC 
GGCACGGACA CGGCACTACA TCGCATCATC GAGGCAATCG ATGCCATCTC CAGTACAGCC 
TACTCCCATC AGCGCACCTT CATCATGGAG GTCATGGGTC GTCATTGCGG CTATTTAGCT 
CTTGTGGGTG GACTGGCGTG CGAGGCGGAT TTCATATTCA TTCCCGAAAT GCCGCCCAAG 
GTCGATTGGC CAGACAGGCT CTGCTCTCAG CTGGCCCAGG AGCGATCTGC TGGCCAGCGT 
CTGAACATCG TGATTGTGGC CGAGGGAGCC ATGGATCGCG AGGGTCATCC CATCACAGCC 
GAGGATGTAA AGAAGGTGAT CGACGAGCGT CTGAAGCACG ATGCCCGCAT CACTGTCTTG 
GGTCACGTGC AGCGCGGTGG CAATCCCAGT GCCTTCGATC GTATTTTGGC TTGCCGCATG 
GGAGCTGAGG CCACTTTGGC CCTGATGGAG GCCACCAAGG ACTCGGTGCC AGTGGTCATC 
TCTCTGGACG GCAACCAGGC GGTTCGCGTG CCGCTGATGG AGTGCGTAGA GCGCACCCAG 
GCTGTGGCCA AGGCCATGGC GGAGAAACGC TGGGCGGATG CCGTCAAGCT GCGCGGACGC 
TCCTTCGAGC GGAATCTGGA GACCTACAAG ATGCTGACGC GCTTGAAGCC GCCCAAGGAG 
AACTTCGACG CCGATGGCAA GGGAATCGAA GGATACCGCC TAGCTGTGAT GCATATTGGT 
GCTCCGGCTT GCGGTATGAA TGCCGCTGTG CGCAGCTTCG TGCGCAATGC CATCTACCGC 
GGTGATGTGG TTTATGGAAT CAACGACGGT GTCGAGGGTC TGATTGCCGG AAATGTCCGC 
GAGCTGGGCT GGTCGGATGT CTCAGGATGG GTTGGTCAGG GTGGCGCCTA CTTGGGTACC 
AAACGCACAC TGCCTGAGGG CAAGTTCAAG GAGATCGCCG CTCGTCTCAA GGAGTTTAAG 
ATCCAGGGTC TCCTGATCAT TGGTGGCTTT GAGAGTTACC ATGCCGCCGG ACAGATCGCC 
GATCAGCGGG ACAACTACCC ACAGTTCTGC ATCCCCATTG TGGTTATTCC ATCGACGATT 
TCGAACAATG TGCCCGGCAC AGAATTTTCT CTGGGATGCG ACACCGGTTT AAATGAGATT 
ACGGAGATTT GCGACCGTAT CCGTCAGTCG GCACAGGGAA CCAAGCGCCG AGTGTTCGTC 
ATTGAGACGA TGGGTGGCTA CTGTGGCTAT TTGGCCACCT TGGCCGGCTT GGCCGGCGGA 
GCTGATGCCG CCTACATCTA CGAGGAGAAG TTCTCCATCA AAGACCTGCA GCAGGATGTC 
TACCACATGG CCTCCAAGAT GGCCGAGGGC GTCTCCCGCG GTCTAATCCT GCGAAACGAG 
AAGGCTAGCG AGAACTACAG CACGGACTTC ATTTACCGCC TGTACTCGGA GGAGGGCAAG 
GGCCTCTTCA CCTGCCGTAT GAACATCCTG GGTCACATGC AGCAGGGCGG CTCACCCACT 
CCCTTCGACC GCAACATGGG CACCAAAATG GCCGCCAAGT GTGTCGACTG GCTGGCCGCC 
CAGATCAAGG CGAACATCGA CGCCAACGGC GTAGTCAACT GCAAGTCCCC GGACACCGCC 
ACGCTGCTGG GAATCGTGTC GCGGCAGTAC CGCTTCTCAC CGCTGGTCGA CCTCATTGCA 
GAGACGAACT TCGATCAACG CATCCCGAAG AAGCAGTGGT GGCTGCGCCT GCGTCCCCTG 
CTGCGCATCC TGGCCAAGCA CGATTCCGCC TACGAGGAGG AGGGTATGTA CATCACCGTC 
GAGGAGGAGT GTGACACTGA CGCCGTCGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfk ( NM_165746.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724890.1 Drosophila melanogaster phosphofructokinase, transcript variant B (Pfk), mRNA.

Price & Availability↑

CloneID	OFa09467
Clone ID Related Accession (Same CDS sequence)	NM_165746.3
Accession Version	NM_165746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphofructokinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165746.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTCCG AGATTAATCA ACGATTCCTG GCCCGCGGCT CGCAGAAGGA CAAAGGACTG 
GCGGTCTTCA CCAGCGGCGG GGATTCCCAG GGCATGAATG CCGCCGTGCG AGCCTGTGTC 
CGTATGGCCA TCTATTTGGG CTGCAAGGTC TACTTCATTC GTGAAGGCTA CCAGGGCATG 
GTGGATGGTG GCGACTGCAT CCAGGAAGCC AACTGGGCCT CAGTCTCATC CATTATCCAT 
CGTGGTGGCA CCATCATTGG CTCCGCCCGT TGCCAGGACT TCCGTGAGCG TCAGGGTCGC 
TTGAAAGCCG CTAACAACCT GATTCAGCGA GGAATCACCA ATCTGGTGGT CATTGGAGGC 
GATGGCTCCC TCACGGGCGC CAATCTGTTC CGTCAGGAGT GGTCCAGCCT GTTGGACGAG 
CTGGTCAAGA ACAAGACCAT TACCACCGAG CAGCAGGAGA AGTTCAATGT CCTGCACATC 
GTTGGATTGG TTGGCTCCAT CGACAACGAT TTCTGTGGCA CTGACATGAC CATCGGCACG 
GACACGGCAC TACATCGCAT CATCGAGGCA ATCGATGCCA TCTCCAGTAC AGCCTACTCC 
CATCAGCGCA CCTTCATCAT GGAGGTCATG GGTCGTCATT GCGGCTATTT AGCTCTTGTG 
GGTGGACTGG CGTGCGAGGC GGATTTCATA TTCATTCCCG AAATGCCGCC CAAGGTCGAT 
TGGCCAGACA GGCTCTGCTC TCAGCTGGCC CAGGAGCGAT CTGCTGGCCA GCGTCTGAAC 
ATCGTGATTG TGGCCGAGGG AGCCATGGAT CGCGAGGGTC ATCCCATCAC AGCCGAGGAT 
GTAAAGAAGG TGATCGACGA GCGTCTGAAG CACGATGCCC GCATCACTGT CTTGGGTCAC 
GTGCAGCGCG GTGGCAATCC CAGTGCCTTC GATCGTATTT TGGCTTGCCG CATGGGAGCT 
GAGGCCACTT TGGCCCTGAT GGAGGCCACC AAGGACTCGG TGCCAGTGGT CATCTCTCTG 
GACGGCAACC AGGCGGTTCG CGTGCCGCTG ATGGAGTGCG TAGAGCGCAC CCAGGCTGTG 
GCCAAGGCCA TGGCGGAGAA ACGCTGGGCG GATGCCGTCA AGCTGCGCGG ACGCTCCTTC 
GAGCGGAATC TGGAGACCTA CAAGATGCTG ACGCGCTTGA AGCCGCCCAA GGAGAACTTC 
GACGCCGATG GCAAGGGAAT CGAAGGATAC CGCCTAGCTG TGATGCATAT TGGTGCTCCG 
GCTTGCGGTA TGAATGCCGC TGTGCGCAGC TTCGTGCGCA ATGCCATCTA CCGCGGTGAT 
GTGGTTTATG GAATCAACGA CGGTGTCGAG GGTCTGATTG CCGGAAATGT CCGCGAGCTG 
GGCTGGTCGG ATGTCTCAGG ATGGGTTGGT CAGGGTGGCG CCTACTTGGG TACCAAACGC 
ACACTGCCTG AGGGCAAGTT CAAGGAGATC GCCGCTCGTC TCAAGGAGTT TAAGATCCAG 
GGTCTCCTGA TCATTGGTGG CTTTGAGAGT TACCATGCCG CCGGACAGAT CGCCGATCAG 
CGGGACAACT ACCCACAGTT CTGCATCCCC ATTGTGGTTA TTCCATCGAC GATTTCGAAC 
AATGTGCCCG GCACAGAATT TTCTCTGGGA TGCGACACCG GTTTAAATGA GATTACGGAG 
ATTTGCGACC GTATCCGTCA GTCGGCACAG GGAACCAAGC GCCGAGTGTT CGTCATTGAG 
ACGATGGGTG GCTACTGTGG CTATTTGGCC ACCTTGGCCG GCTTGGCCGG CGGAGCTGAT 
GCCGCCTACA TCTACGAGGA GAAGTTCTCC ATCAAAGACC TGCAGCAGGA TGTCTACCAC 
ATGGCCTCCA AGATGGCCGA GGGCGTCTCC CGCGGTCTAA TCCTGCGAAA CGAGAAGGCT 
AGCGAGAACT ACAGCACGGA CTTCATTTAC CGCCTGTACT CGGAGGAGGG CAAGGGCCTC 
TTCACCTGCC GTATGAACAT CCTGGGTCAC ATGCAGCAGG GCGGCTCACC CACTCCCTTC 
GACCGCAACA TGGGCACCAA AATGGCCGCC AAGTGTGTCG ACTGGCTGGC CGCCCAGATC 
AAGGCGAACA TCGACGCCAA CGGCGTAGTC AACTGCAAGT CCCCGGACAC CGCCACGCTG 
CTGGGAATCG TGTCGCGGCA GTACCGCTTC TCACCGCTGG TCGACCTCAT TGCAGAGACG 
AACTTCGATC AACGCATCCC GAAGAAGCAG TGGTGGCTGC GCCTGCGTCC CCTGCTGCGC 
ATCCTGGCCA AGCACGATTC CGCCTACGAG GAGGAGGGTA TGTACATCAC CGTCGAGGAG 
GAGTGTGACA CTGACGCCGT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724890.1
CDS	560..2926
Translation	MNSEINQRFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA

Target ORF information:

RefSeq Version	NM_165746.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphofructokinase, transcript variant B (Pfk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165746.3

ORF Insert Sequence:

ATGAATTCCG AGATTAATCA ACGATTCCTG GCCCGCGGCT CGCAGAAGGA CAAAGGACTG 
GCGGTCTTCA CCAGCGGCGG GGATTCCCAG GGCATGAATG CCGCCGTGCG AGCCTGTGTC 
CGTATGGCCA TCTATTTGGG CTGCAAGGTC TACTTCATTC GTGAAGGCTA CCAGGGCATG 
GTGGATGGTG GCGACTGCAT CCAGGAAGCC AACTGGGCCT CAGTCTCATC CATTATCCAT 
CGTGGTGGCA CCATCATTGG CTCCGCCCGT TGCCAGGACT TCCGTGAGCG TCAGGGTCGC 
TTGAAAGCCG CTAACAACCT GATTCAGCGA GGAATCACCA ATCTGGTGGT CATTGGAGGC 
GATGGCTCCC TCACGGGCGC CAATCTGTTC CGTCAGGAGT GGTCCAGCCT GTTGGACGAG 
CTGGTCAAGA ACAAGACCAT TACCACCGAG CAGCAGGAGA AGTTCAATGT CCTGCACATC 
GTTGGATTGG TTGGCTCCAT CGACAACGAT TTCTGTGGCA CTGACATGAC CATCGGCACG 
GACACGGCAC TACATCGCAT CATCGAGGCA ATCGATGCCA TCTCCAGTAC AGCCTACTCC 
CATCAGCGCA CCTTCATCAT GGAGGTCATG GGTCGTCATT GCGGCTATTT AGCTCTTGTG 
GGTGGACTGG CGTGCGAGGC GGATTTCATA TTCATTCCCG AAATGCCGCC CAAGGTCGAT 
TGGCCAGACA GGCTCTGCTC TCAGCTGGCC CAGGAGCGAT CTGCTGGCCA GCGTCTGAAC 
ATCGTGATTG TGGCCGAGGG AGCCATGGAT CGCGAGGGTC ATCCCATCAC AGCCGAGGAT 
GTAAAGAAGG TGATCGACGA GCGTCTGAAG CACGATGCCC GCATCACTGT CTTGGGTCAC 
GTGCAGCGCG GTGGCAATCC CAGTGCCTTC GATCGTATTT TGGCTTGCCG CATGGGAGCT 
GAGGCCACTT TGGCCCTGAT GGAGGCCACC AAGGACTCGG TGCCAGTGGT CATCTCTCTG 
GACGGCAACC AGGCGGTTCG CGTGCCGCTG ATGGAGTGCG TAGAGCGCAC CCAGGCTGTG 
GCCAAGGCCA TGGCGGAGAA ACGCTGGGCG GATGCCGTCA AGCTGCGCGG ACGCTCCTTC 
GAGCGGAATC TGGAGACCTA CAAGATGCTG ACGCGCTTGA AGCCGCCCAA GGAGAACTTC 
GACGCCGATG GCAAGGGAAT CGAAGGATAC CGCCTAGCTG TGATGCATAT TGGTGCTCCG 
GCTTGCGGTA TGAATGCCGC TGTGCGCAGC TTCGTGCGCA ATGCCATCTA CCGCGGTGAT 
GTGGTTTATG GAATCAACGA CGGTGTCGAG GGTCTGATTG CCGGAAATGT CCGCGAGCTG 
GGCTGGTCGG ATGTCTCAGG ATGGGTTGGT CAGGGTGGCG CCTACTTGGG TACCAAACGC 
ACACTGCCTG AGGGCAAGTT CAAGGAGATC GCCGCTCGTC TCAAGGAGTT TAAGATCCAG 
GGTCTCCTGA TCATTGGTGG CTTTGAGAGT TACCATGCCG CCGGACAGAT CGCCGATCAG 
CGGGACAACT ACCCACAGTT CTGCATCCCC ATTGTGGTTA TTCCATCGAC GATTTCGAAC 
AATGTGCCCG GCACAGAATT TTCTCTGGGA TGCGACACCG GTTTAAATGA GATTACGGAG 
ATTTGCGACC GTATCCGTCA GTCGGCACAG GGAACCAAGC GCCGAGTGTT CGTCATTGAG 
ACGATGGGTG GCTACTGTGG CTATTTGGCC ACCTTGGCCG GCTTGGCCGG CGGAGCTGAT 
GCCGCCTACA TCTACGAGGA GAAGTTCTCC ATCAAAGACC TGCAGCAGGA TGTCTACCAC 
ATGGCCTCCA AGATGGCCGA GGGCGTCTCC CGCGGTCTAA TCCTGCGAAA CGAGAAGGCT 
AGCGAGAACT ACAGCACGGA CTTCATTTAC CGCCTGTACT CGGAGGAGGG CAAGGGCCTC 
TTCACCTGCC GTATGAACAT CCTGGGTCAC ATGCAGCAGG GCGGCTCACC CACTCCCTTC 
GACCGCAACA TGGGCACCAA AATGGCCGCC AAGTGTGTCG ACTGGCTGGC CGCCCAGATC 
AAGGCGAACA TCGACGCCAA CGGCGTAGTC AACTGCAAGT CCCCGGACAC CGCCACGCTG 
CTGGGAATCG TGTCGCGGCA GTACCGCTTC TCACCGCTGG TCGACCTCAT TGCAGAGACG 
AACTTCGATC AACGCATCCC GAAGAAGCAG TGGTGGCTGC GCCTGCGTCC CCTGCTGCGC 
ATCCTGGCCA AGCACGATTC CGCCTACGAG GAGGAGGGTA TGTACATCAC CGTCGAGGAG 
GAGTGTGACA CTGACGCCGT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfk ( NM_165747.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724891.2 Drosophila melanogaster phosphofructokinase, transcript variant C (Pfk), mRNA.

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CloneID	OFa09468
Clone ID Related Accession (Same CDS sequence)	NM_165747.4
Accession Version	NM_165747.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphofructokinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165747.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTCCG AGATTAATCA ACGATTCCTG GCCCGCGGCT CGCAGAAGGA CAAAGGACTG 
GCGGTCTTCA CCAGCGGCGG GGATTCCCAG GGCATGAATG CCGCCGTGCG AGCCTGTGTC 
CGTATGGCCA TCTATTTGGG CTGCAAGGTC TACTTCATTC GTGAAGGCTA CCAGGGCATG 
GTGGATGGTG GCGACTGCAT CCAGGAAGCC AACTGGGCCT CAGTCTCATC CATTATCCAT 
CGTGGTGGCA CCATCATTGG CTCCGCCCGT TGCCAGGACT TCCGTGAGCG TCAGGGTCGC 
TTGAAAGCCG CTAACAACCT GATTCAGCGA GGAATCACCA ATCTGGTGGT CATTGGAGGC 
GATGGCTCCC TCACGGGCGC CAATCTGTTC CGTCAGGAGT GGTCCAGCCT GTTGGACGAG 
CTGGTCAAGA ACAAGACCAT TACCACCGAG CAGCAGGAGA AGTTCAATGT CCTGCACATC 
GTTGGATTGG TTGGCTCCAT CGACAACGAT TTCTGTGGCA CTGACATGAC CATCGGCACG 
GACACGGCAC TACATCGCAT CATCGAGGCA ATCGATGCCA TCTCCAGTAC AGCCTACTCC 
CATCAGCGCA CCTTCATCAT GGAGGTCATG GGTCGTCATT GCGGTTACTT AGCCATATCG 
GCAGCCATAG CCACAGAGGC TGATTTTATG TTCATACCCG AGGAGCCGGT GTCAGTCAAT 
TGGAAGGACG AGATCTGTGT CAAGCTCCAC CAGGAGCGAT CTGCTGGCCA GCGTCTGAAC 
ATCGTGATTG TGGCCGAGGG AGCCATGGAT CGCGAGGGTC ATCCCATCAC AGCCGAGGAT 
GTAAAGAAGG TGATCGACGA GCGTCTGAAG CACGATGCCC GCATCACTGT CTTGGGTCAC 
GTGCAGCGCG GTGGCAATCC CAGTGCCTTC GATCGTATTT TGGCTTGCCG CATGGGAGCT 
GAGGCCACTT TGGCCCTGAT GGAGGCCACC AAGGACTCGG TGCCAGTGGT CATCTCTCTG 
GACGGCAACC AGGCGGTTCG CGTGCCGCTG ATGGAGTGCG TAGAGCGCAC CCAGGCTGTG 
GCCAAGGCCA TGGCGGAGAA ACGCTGGGCG GATGCCGTCA AGCTGCGCGG ACGCTCCTTC 
GAGCGGAATC TGGAGACCTA CAAGATGCTG ACGCGCTTGA AGCCGCCCAA GGAGAACTTC 
GACGCCGATG GCAAGGGAAT CGAAGGATAC CGCCTAGCTG TGATGCATAT TGGTGCTCCG 
GCTTGCGGTA TGAATGCCGC TGTGCGCAGC TTCGTGCGCA ATGCCATCTA CCGCGGTGAT 
GTGGTTTATG GAATCAACGA CGGTGTCGAG GGTCTGATTG CCGGAAATGT CCGCGAGCTG 
GGCTGGTCGG ATGTCTCAGG ATGGGTTGGT CAGGGTGGCG CCTACTTGGG TACCAAACGC 
ACACTGCCTG AGGGCAAGTT CAAGGAGATC GCCGCTCGTC TCAAGGAGTT TAAGATCCAG 
GGTCTCCTGA TCATTGGTGG CTTTGAGAGT TACCATGCCG CCGGACAGAT CGCCGATCAG 
CGGGACAACT ACCCACAGTT CTGCATCCCC ATTGTGGTTA TTCCATCGAC GATTTCGAAC 
AATGTGCCCG GCACAGAATT TTCTCTGGGA TGCGACACCG GTTTAAATGA GATTACGGAG 
ATTTGCGACC GTATCCGTCA GTCGGCACAG GGAACCAAGC GCCGAGTGTT CGTCATTGAG 
ACGATGGGTG GCTACTGTGG CTATTTGGCC ACCTTGGCCG GCTTGGCCGG CGGAGCTGAT 
GCCGCCTACA TCTACGAGGA GAAGTTCTCC ATCAAAGACC TGCAGCAGGA TGTCTACCAC 
ATGGCCTCCA AGATGGCCGA GGGCGTCTCC CGCGGTCTAA TCCTGCGAAA CGAGAAGGCT 
AGCGAGAACT ACAGCACGGA CTTCATTTAC CGCCTGTACT CGGAGGAGGG CAAGGGCCTC 
TTCACCTGCC GTATGAACAT CCTGGGTCAC ATGCAGCAGG GCGGCTCACC CACTCCCTTC 
GACCGCAACA TGGGCACCAA AATGGCCGCC AAGTGTGTCG ACTGGCTGGC CGCCCAGATC 
AAGGCGAACA TCGACGCCAA CGGCGTAGTC AACTGCAAGT CCCCGGACAC CGCCACGCTG 
CTGGGAATCG TGTCGCGGCA GTACCGCTTC TCACCGCTGG TCGACCTCAT TGCAGAGACG 
AACTTCGATC AACGCATCCC GAAGAAGCAG TGGTGGCTGC GCCTGCGTCC CCTGCTGCGC 
ATCCTGGCCA AGCACGATTC CGCCTACGAG GAGGAGGGTA TGTACATCAC CGTCGAGGAG 
GAGTGTGACA CTGACGCCGT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724891.2
CDS	560..2926
Translation	MNSEINQRFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLAISAAIATEADFMFIPEEPVSVNWKDEICVKLHQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA

Target ORF information:

RefSeq Version	NM_165747.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphofructokinase, transcript variant C (Pfk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165747.4

ORF Insert Sequence:

ATGAATTCCG AGATTAATCA ACGATTCCTG GCCCGCGGCT CGCAGAAGGA CAAAGGACTG 
GCGGTCTTCA CCAGCGGCGG GGATTCCCAG GGCATGAATG CCGCCGTGCG AGCCTGTGTC 
CGTATGGCCA TCTATTTGGG CTGCAAGGTC TACTTCATTC GTGAAGGCTA CCAGGGCATG 
GTGGATGGTG GCGACTGCAT CCAGGAAGCC AACTGGGCCT CAGTCTCATC CATTATCCAT 
CGTGGTGGCA CCATCATTGG CTCCGCCCGT TGCCAGGACT TCCGTGAGCG TCAGGGTCGC 
TTGAAAGCCG CTAACAACCT GATTCAGCGA GGAATCACCA ATCTGGTGGT CATTGGAGGC 
GATGGCTCCC TCACGGGCGC CAATCTGTTC CGTCAGGAGT GGTCCAGCCT GTTGGACGAG 
CTGGTCAAGA ACAAGACCAT TACCACCGAG CAGCAGGAGA AGTTCAATGT CCTGCACATC 
GTTGGATTGG TTGGCTCCAT CGACAACGAT TTCTGTGGCA CTGACATGAC CATCGGCACG 
GACACGGCAC TACATCGCAT CATCGAGGCA ATCGATGCCA TCTCCAGTAC AGCCTACTCC 
CATCAGCGCA CCTTCATCAT GGAGGTCATG GGTCGTCATT GCGGTTACTT AGCCATATCG 
GCAGCCATAG CCACAGAGGC TGATTTTATG TTCATACCCG AGGAGCCGGT GTCAGTCAAT 
TGGAAGGACG AGATCTGTGT CAAGCTCCAC CAGGAGCGAT CTGCTGGCCA GCGTCTGAAC 
ATCGTGATTG TGGCCGAGGG AGCCATGGAT CGCGAGGGTC ATCCCATCAC AGCCGAGGAT 
GTAAAGAAGG TGATCGACGA GCGTCTGAAG CACGATGCCC GCATCACTGT CTTGGGTCAC 
GTGCAGCGCG GTGGCAATCC CAGTGCCTTC GATCGTATTT TGGCTTGCCG CATGGGAGCT 
GAGGCCACTT TGGCCCTGAT GGAGGCCACC AAGGACTCGG TGCCAGTGGT CATCTCTCTG 
GACGGCAACC AGGCGGTTCG CGTGCCGCTG ATGGAGTGCG TAGAGCGCAC CCAGGCTGTG 
GCCAAGGCCA TGGCGGAGAA ACGCTGGGCG GATGCCGTCA AGCTGCGCGG ACGCTCCTTC 
GAGCGGAATC TGGAGACCTA CAAGATGCTG ACGCGCTTGA AGCCGCCCAA GGAGAACTTC 
GACGCCGATG GCAAGGGAAT CGAAGGATAC CGCCTAGCTG TGATGCATAT TGGTGCTCCG 
GCTTGCGGTA TGAATGCCGC TGTGCGCAGC TTCGTGCGCA ATGCCATCTA CCGCGGTGAT 
GTGGTTTATG GAATCAACGA CGGTGTCGAG GGTCTGATTG CCGGAAATGT CCGCGAGCTG 
GGCTGGTCGG ATGTCTCAGG ATGGGTTGGT CAGGGTGGCG CCTACTTGGG TACCAAACGC 
ACACTGCCTG AGGGCAAGTT CAAGGAGATC GCCGCTCGTC TCAAGGAGTT TAAGATCCAG 
GGTCTCCTGA TCATTGGTGG CTTTGAGAGT TACCATGCCG CCGGACAGAT CGCCGATCAG 
CGGGACAACT ACCCACAGTT CTGCATCCCC ATTGTGGTTA TTCCATCGAC GATTTCGAAC 
AATGTGCCCG GCACAGAATT TTCTCTGGGA TGCGACACCG GTTTAAATGA GATTACGGAG 
ATTTGCGACC GTATCCGTCA GTCGGCACAG GGAACCAAGC GCCGAGTGTT CGTCATTGAG 
ACGATGGGTG GCTACTGTGG CTATTTGGCC ACCTTGGCCG GCTTGGCCGG CGGAGCTGAT 
GCCGCCTACA TCTACGAGGA GAAGTTCTCC ATCAAAGACC TGCAGCAGGA TGTCTACCAC 
ATGGCCTCCA AGATGGCCGA GGGCGTCTCC CGCGGTCTAA TCCTGCGAAA CGAGAAGGCT 
AGCGAGAACT ACAGCACGGA CTTCATTTAC CGCCTGTACT CGGAGGAGGG CAAGGGCCTC 
TTCACCTGCC GTATGAACAT CCTGGGTCAC ATGCAGCAGG GCGGCTCACC CACTCCCTTC 
GACCGCAACA TGGGCACCAA AATGGCCGCC AAGTGTGTCG ACTGGCTGGC CGCCCAGATC 
AAGGCGAACA TCGACGCCAA CGGCGTAGTC AACTGCAAGT CCCCGGACAC CGCCACGCTG 
CTGGGAATCG TGTCGCGGCA GTACCGCTTC TCACCGCTGG TCGACCTCAT TGCAGAGACG 
AACTTCGATC AACGCATCCC GAAGAAGCAG TGGTGGCTGC GCCTGCGTCC CCTGCTGCGC 
ATCCTGGCCA AGCACGATTC CGCCTACGAG GAGGAGGGTA TGTACATCAC CGTCGAGGAG 
GAGTGTGACA CTGACGCCGT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfk ( NM_001273918.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260847.1 Drosophila melanogaster phosphofructokinase, transcript variant E (Pfk), mRNA.

Price & Availability↑

CloneID	OFa12875
Clone ID Related Accession (Same CDS sequence)	NM_001273918.1
Accession Version	NM_001273918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphofructokinase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCAA TAAAATTTCG AGTATTTACC AAATTAAAAC CTATCTGTTC GATTGTTTGG 
AGATTTTTGG AAATCAATGG ACGGATACCC ATTTGCCGCC ATTTTCATGG TCCTACAACA 
TTTCGCTTAG AAATCTCAAA CAAAACACCT CCGATTAGGC AAAAGCTCAC ATTTCCAAAC 
ATCGGCATCC AATGCACACG AAGTCATCAT TTGTGTTGTC CTCGAGATAT TTCAGGAAAT 
ACTCTTTTAA GTGTAAAATT TAATTGCAAA CGACATTGTA TAAAGTTGCG CAGTGATTCT 
GGTGATCAAA AGAATGATAG TCCTGGTGAA AAAAACATAC AAAAAGACAA GTCTGCGCAA 
CGATGTGGAA AACCAATAAA CAATCTGCAC AACGGATTTC TTAATGCTGT GAACTATTCT 
GAAAAGAATG CGGTGAAAAA GAAAAAGAGT GCACCAAAAA GGAAATGTGG CAAATCGGTG 
GATGAGCTGC GAAAGTGTTT AAGAACCATG CAGGATGTTA TTGATTTTGT ACATCCAGTC 
AAGCCTTTTG TCTACTTCAT TCGTGAAGGC TACCAGGGCA TGGTGGATGG TGGCGACTGC 
ATCCAGGAAG CCAACTGGGC CTCAGTCTCA TCCATTATCC ATCGTGGTGG CACCATCATT 
GGCTCCGCCC GTTGCCAGGA CTTCCGTGAG CGTCAGGGTC GCTTGAAAGC CGCTAACAAC 
CTGATTCAGC GAGGAATCAC CAATCTGGTG GTCATTGGAG GCGATGGCTC CCTCACGGGC 
GCCAATCTGT TCCGTCAGGA GTGGTCCAGC CTGTTGGACG AGCTGGTCAA GAACAAGACC 
ATTACCACCG AGCAGCAGGA GAAGTTCAAT GTCCTGCACA TCGTTGGATT GGTTGGCTCC 
ATCGACAACG ATTTCTGTGG CACTGACATG ACCATCGGCA CGGACACGGC ACTACATCGC 
ATCATCGAGG CAATCGATGC CATCTCCAGT ACAGCCTACT CCCATCAGCG CACCTTCATC 
ATGGAGGTCA TGGGTCGTCA TTGCGGCTAT CTTTCGGTGG TAGCTGGCAT TATCTCCGAG 
GCAGACTACG TCTTTCTGCC CGAGTCTCCT CCGCAGGCCG ATTGGCCCGA TCGACTTGTC 
CTTAAATTGG AACAGGAGCG ATCTGCTGGC CAGCGTCTGA ACATCGTGAT TGTGGCCGAG 
GGAGCCATGG ATCGCGAGGG TCATCCCATC ACAGCCGAGG ATGTAAAGAA GGTGATCGAC 
GAGCGTCTGA AGCACGATGC CCGCATCACT GTCTTGGGTC ACGTGCAGCG CGGTGGCAAT 
CCCAGTGCCT TCGATCGTAT TTTGGCTTGC CGCATGGGAG CTGAGGCCAC TTTGGCCCTG 
ATGGAGGCCA CCAAGGACTC GGTGCCAGTG GTCATCTCTC TGGACGGCAA CCAGGCGGTT 
CGCGTGCCGC TGATGGAGTG CGTAGAGCGC ACCCAGGCTG TGGCCAAGGC CATGGCGGAG 
AAACGCTGGG CGGATGCCGT CAAGCTGCGC GGACGCTCCT TCGAGCGGAA TCTGGAGACC 
TACAAGATGC TGACGCGCTT GAAGCCGCCC AAGGAGAACT TCGACGCCGA TGGCAAGGGA 
ATCGAAGGAT ACCGCCTAGC TGTGATGCAT ATTGGTGCTC CGGCTTGCGG TATGAATGCC 
GCTGTGCGCA GCTTCGTGCG CAATGCCATC TACCGCGGTG ATGTGGTTTA TGGAATCAAC 
GACGGTGTCG AGGGTCTGAT TGCCGGAAAT GTCCGCGAGC TGGGCTGGTC GGATGTCTCA 
GGATGGGTTG GTCAGGGTGG CGCCTACTTG GGTACCAAAC GCACACTGCC TGAGGGCAAG 
TTCAAGGAGA TCGCCGCTCG TCTCAAGGAG TTTAAGATCC AGGGTCTCCT GATCATTGGT 
GGCTTTGAGA GTTACCATGC CGCCGGACAG ATCGCCGATC AGCGGGACAA CTACCCACAG 
TTCTGCATCC CCATTGTGGT TATTCCATCG ACGATTTCGA ACAATGTGCC CGGCACAGAA 
TTTTCTCTGG GATGCGACAC CGGTTTAAAT GAGATTACGG AGATTTGCGA CCGTATCCGT 
CAGTCGGCAC AGGGAACCAA GCGCCGAGTG TTCGTCATTG AGACGATGGG TGGCTACTGT 
GGCTATTTGG CCACCTTGGC CGGCTTGGCC GGCGGAGCTG ATGCCGCCTA CATCTACGAG 
GAGAAGTTCT CCATCAAAGA CCTGCAGCAG GATGTCTACC ACATGGCCTC CAAGATGGCC 
GAGGGCGTCT CCCGCGGTCT AATCCTGCGA AACGAGAAGG CTAGCGAGAA CTACAGCACG 
GACTTCATTT ACCGCCTGTA CTCGGAGGAG GGCAAGGGCC TCTTCACCTG CCGTATGAAC 
ATCCTGGGTC ACATGCAGCA GGGCGGCTCA CCCACTCCCT TCGACCGCAA CATGGGCACC 
AAAATGGCCG CCAAGTGTGT CGACTGGCTG GCCGCCCAGA TCAAGGCGAA CATCGACGCC 
AACGGCGTAG TCAACTGCAA GTCCCCGGAC ACCGCCACGC TGCTGGGAAT CGTGTCGCGG 
CAGTACCGCT TCTCACCGCT GGTCGACCTC ATTGCAGAGA CGAACTTCGA TCAACGCATC 
CCGAAGAAGC AGTGGTGGCT GCGCCTGCGT CCCCTGCTGC GCATCCTGGC CAAGCACGAT 
TCCGCCTACG AGGAGGAGGG TATGTACATC ACCGTCGAGG AGGAGTGTGA CACTGACGCC 
GTCGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260847.1
CDS	103..2871
Translation	MHSIKFRVFTKLKPICSIVWRFLEINGRIPICRHFHGPTTFRLEISNKTPPIRQKLTFPNIGIQCTRSHHLCCPRDISGNTLLSVKFNCKRHCIKLRSDSGDQKNDSPGEKNIQKDKSAQRCGKPINNLHNGFLNAVNYSEKNAVKKKKSAPKRKCGKSVDELRKCLRTMQDVIDFVHPVKPFVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLSVVAGIISEADYVFLPESPPQADWPDRLVLKLEQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA

Target ORF information:

RefSeq Version	NM_001273918.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphofructokinase, transcript variant E (Pfk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273918.1

ORF Insert Sequence:

ATGCATTCAA TAAAATTTCG AGTATTTACC AAATTAAAAC CTATCTGTTC GATTGTTTGG 
AGATTTTTGG AAATCAATGG ACGGATACCC ATTTGCCGCC ATTTTCATGG TCCTACAACA 
TTTCGCTTAG AAATCTCAAA CAAAACACCT CCGATTAGGC AAAAGCTCAC ATTTCCAAAC 
ATCGGCATCC AATGCACACG AAGTCATCAT TTGTGTTGTC CTCGAGATAT TTCAGGAAAT 
ACTCTTTTAA GTGTAAAATT TAATTGCAAA CGACATTGTA TAAAGTTGCG CAGTGATTCT 
GGTGATCAAA AGAATGATAG TCCTGGTGAA AAAAACATAC AAAAAGACAA GTCTGCGCAA 
CGATGTGGAA AACCAATAAA CAATCTGCAC AACGGATTTC TTAATGCTGT GAACTATTCT 
GAAAAGAATG CGGTGAAAAA GAAAAAGAGT GCACCAAAAA GGAAATGTGG CAAATCGGTG 
GATGAGCTGC GAAAGTGTTT AAGAACCATG CAGGATGTTA TTGATTTTGT ACATCCAGTC 
AAGCCTTTTG TCTACTTCAT TCGTGAAGGC TACCAGGGCA TGGTGGATGG TGGCGACTGC 
ATCCAGGAAG CCAACTGGGC CTCAGTCTCA TCCATTATCC ATCGTGGTGG CACCATCATT 
GGCTCCGCCC GTTGCCAGGA CTTCCGTGAG CGTCAGGGTC GCTTGAAAGC CGCTAACAAC 
CTGATTCAGC GAGGAATCAC CAATCTGGTG GTCATTGGAG GCGATGGCTC CCTCACGGGC 
GCCAATCTGT TCCGTCAGGA GTGGTCCAGC CTGTTGGACG AGCTGGTCAA GAACAAGACC 
ATTACCACCG AGCAGCAGGA GAAGTTCAAT GTCCTGCACA TCGTTGGATT GGTTGGCTCC 
ATCGACAACG ATTTCTGTGG CACTGACATG ACCATCGGCA CGGACACGGC ACTACATCGC 
ATCATCGAGG CAATCGATGC CATCTCCAGT ACAGCCTACT CCCATCAGCG CACCTTCATC 
ATGGAGGTCA TGGGTCGTCA TTGCGGCTAT CTTTCGGTGG TAGCTGGCAT TATCTCCGAG 
GCAGACTACG TCTTTCTGCC CGAGTCTCCT CCGCAGGCCG ATTGGCCCGA TCGACTTGTC 
CTTAAATTGG AACAGGAGCG ATCTGCTGGC CAGCGTCTGA ACATCGTGAT TGTGGCCGAG 
GGAGCCATGG ATCGCGAGGG TCATCCCATC ACAGCCGAGG ATGTAAAGAA GGTGATCGAC 
GAGCGTCTGA AGCACGATGC CCGCATCACT GTCTTGGGTC ACGTGCAGCG CGGTGGCAAT 
CCCAGTGCCT TCGATCGTAT TTTGGCTTGC CGCATGGGAG CTGAGGCCAC TTTGGCCCTG 
ATGGAGGCCA CCAAGGACTC GGTGCCAGTG GTCATCTCTC TGGACGGCAA CCAGGCGGTT 
CGCGTGCCGC TGATGGAGTG CGTAGAGCGC ACCCAGGCTG TGGCCAAGGC CATGGCGGAG 
AAACGCTGGG CGGATGCCGT CAAGCTGCGC GGACGCTCCT TCGAGCGGAA TCTGGAGACC 
TACAAGATGC TGACGCGCTT GAAGCCGCCC AAGGAGAACT TCGACGCCGA TGGCAAGGGA 
ATCGAAGGAT ACCGCCTAGC TGTGATGCAT ATTGGTGCTC CGGCTTGCGG TATGAATGCC 
GCTGTGCGCA GCTTCGTGCG CAATGCCATC TACCGCGGTG ATGTGGTTTA TGGAATCAAC 
GACGGTGTCG AGGGTCTGAT TGCCGGAAAT GTCCGCGAGC TGGGCTGGTC GGATGTCTCA 
GGATGGGTTG GTCAGGGTGG CGCCTACTTG GGTACCAAAC GCACACTGCC TGAGGGCAAG 
TTCAAGGAGA TCGCCGCTCG TCTCAAGGAG TTTAAGATCC AGGGTCTCCT GATCATTGGT 
GGCTTTGAGA GTTACCATGC CGCCGGACAG ATCGCCGATC AGCGGGACAA CTACCCACAG 
TTCTGCATCC CCATTGTGGT TATTCCATCG ACGATTTCGA ACAATGTGCC CGGCACAGAA 
TTTTCTCTGG GATGCGACAC CGGTTTAAAT GAGATTACGG AGATTTGCGA CCGTATCCGT 
CAGTCGGCAC AGGGAACCAA GCGCCGAGTG TTCGTCATTG AGACGATGGG TGGCTACTGT 
GGCTATTTGG CCACCTTGGC CGGCTTGGCC GGCGGAGCTG ATGCCGCCTA CATCTACGAG 
GAGAAGTTCT CCATCAAAGA CCTGCAGCAG GATGTCTACC ACATGGCCTC CAAGATGGCC 
GAGGGCGTCT CCCGCGGTCT AATCCTGCGA AACGAGAAGG CTAGCGAGAA CTACAGCACG 
GACTTCATTT ACCGCCTGTA CTCGGAGGAG GGCAAGGGCC TCTTCACCTG CCGTATGAAC 
ATCCTGGGTC ACATGCAGCA GGGCGGCTCA CCCACTCCCT TCGACCGCAA CATGGGCACC 
AAAATGGCCG CCAAGTGTGT CGACTGGCTG GCCGCCCAGA TCAAGGCGAA CATCGACGCC 
AACGGCGTAG TCAACTGCAA GTCCCCGGAC ACCGCCACGC TGCTGGGAAT CGTGTCGCGG 
CAGTACCGCT TCTCACCGCT GGTCGACCTC ATTGCAGAGA CGAACTTCGA TCAACGCATC 
CCGAAGAAGC AGTGGTGGCT GCGCCTGCGT CCCCTGCTGC GCATCCTGGC CAAGCACGAT 
TCCGCCTACG AGGAGGAGGG TATGTACATC ACCGTCGAGG AGGAGTGTGA CACTGACGCC 
GTCGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfk ( NM_001259305.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246234.1 Drosophila melanogaster phosphofructokinase, transcript variant D (Pfk), mRNA.

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CloneID	OFa12878
Clone ID Related Accession (Same CDS sequence)	NM_001259305.1
Accession Version	NM_001259305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphofructokinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCAA TAAAATTTCG AGTATTTACC AAATTAAAAC CTATATTTTT GGAAATCAAT 
GGACGGATAC CCATTTGCCG CCATTTTCAT GGTCCTACAA CATTTCGCTT AGAAATCTCA 
AACAAAACAC CTCCGATTAG GCAAAAGCTC ACATTTCCAA ACATCGGCAT CCAATGCACA 
CGAAGTCATC ATTTGTGTTG TCCTCGAGAT ATTTCAGGAA ATACTCTTTT AAGTGTAAAA 
TTTAATTGCA AACGACATTG TATAAAGTTG CGCAGTGATT CTGGTGATCA AAAGAATGAT 
AGTCCTGGTG AAAAAAACAT ACAAAAAGAC AAGTCTGCGC AACGATGTGG AAAACCAATA 
AACAATCTGC ACAACGGATT TCTTAATGCT GTGAACTATT CTGAAAAGAA TGCGGTGAAA 
AAGAAAAAGA GTGCACCAAA AAGGAAATGT GGCAAATCGG TGGATGAGCT GCGAAAGTGT 
TTAAGAACCA TGCAGGATGT TATTGATTTT GTACATCCAG TCAAGCCTTT TAAGGACAAA 
GGACTGGCGG TCTTCACCAG CGGCGGGGAT TCCCAGGGCA TGAATGCCGC CGTGCGAGCC 
TGTGTCCGTA TGGCCATCTA TTTGGGCTGC AAGGTCTACT TCATTCGTGA AGGCTACCAG 
GGCATGGTGG ATGGTGGCGA CTGCATCCAG GAAGCCAACT GGGCCTCAGT CTCATCCATT 
ATCCATCGTG GTGGCACCAT CATTGGCTCC GCCCGTTGCC AGGACTTCCG TGAGCGTCAG 
GGTCGCTTGA AAGCCGCTAA CAACCTGATT CAGCGAGGAA TCACCAATCT GGTGGTCATT 
GGAGGCGATG GCTCCCTCAC GGGCGCCAAT CTGTTCCGTC AGGAGTGGTC CAGCCTGTTG 
GACGAGCTGG TCAAGAACAA GACCATTACC ACCGAGCAGC AGGAGAAGTT CAATGTCCTG 
CACATCGTTG GATTGGTTGG CTCCATCGAC AACGATTTCT GTGGCACTGA CATGACCATC 
GGCACGGACA CGGCACTACA TCGCATCATC GAGGCAATCG ATGCCATCTC CAGTACAGCC 
TACTCCCATC AGCGCACCTT CATCATGGAG GTCATGGGTC GTCATTGCGG CTATCTTTCG 
GTGGTAGCTG GCATTATCTC CGAGGCAGAC TACGTCTTTC TGCCCGAGTC TCCTCCGCAG 
GCCGATTGGC CCGATCGACT TGTCCTTAAA TTGGAACAGG AGCGATCTGC TGGCCAGCGT 
CTGAACATCG TGATTGTGGC CGAGGGAGCC ATGGATCGCG AGGGTCATCC CATCACAGCC 
GAGGATGTAA AGAAGGTGAT CGACGAGCGT CTGAAGCACG ATGCCCGCAT CACTGTCTTG 
GGTCACGTGC AGCGCGGTGG CAATCCCAGT GCCTTCGATC GTATTTTGGC TTGCCGCATG 
GGAGCTGAGG CCACTTTGGC CCTGATGGAG GCCACCAAGG ACTCGGTGCC AGTGGTCATC 
TCTCTGGACG GCAACCAGGC GGTTCGCGTG CCGCTGATGG AGTGCGTAGA GCGCACCCAG 
GCTGTGGCCA AGGCCATGGC GGAGAAACGC TGGGCGGATG CCGTCAAGCT GCGCGGACGC 
TCCTTCGAGC GGAATCTGGA GACCTACAAG ATGCTGACGC GCTTGAAGCC GCCCAAGGAG 
AACTTCGACG CCGATGGCAA GGGAATCGAA GGATACCGCC TAGCTGTGAT GCATATTGGT 
GCTCCGGCTT GCGGTATGAA TGCCGCTGTG CGCAGCTTCG TGCGCAATGC CATCTACCGC 
GGTGATGTGG TTTATGGAAT CAACGACGGT GTCGAGGGTC TGATTGCCGG AAATGTCCGC 
GAGCTGGGCT GGTCGGATGT CTCAGGATGG GTTGGTCAGG GTGGCGCCTA CTTGGGTACC 
AAACGCACAC TGCCTGAGGG CAAGTTCAAG GAGATCGCCG CTCGTCTCAA GGAGTTTAAG 
ATCCAGGGTC TCCTGATCAT TGGTGGCTTT GAGAGTTACC ATGCCGCCGG ACAGATCGCC 
GATCAGCGGG ACAACTACCC ACAGTTCTGC ATCCCCATTG TGGTTATTCC ATCGACGATT 
TCGAACAATG TGCCCGGCAC AGAATTTTCT CTGGGATGCG ACACCGGTTT AAATGAGATT 
ACGGAGATTT GCGACCGTAT CCGTCAGTCG GCACAGGGAA CCAAGCGCCG AGTGTTCGTC 
ATTGAGACGA TGGGTGGCTA CTGTGGCTAT TTGGCCACCT TGGCCGGCTT GGCCGGCGGA 
GCTGATGCCG CCTACATCTA CGAGGAGAAG TTCTCCATCA AAGACCTGCA GCAGGATGTC 
TACCACATGG CCTCCAAGAT GGCCGAGGGC GTCTCCCGCG GTCTAATCCT GCGAAACGAG 
AAGGCTAGCG AGAACTACAG CACGGACTTC ATTTACCGCC TGTACTCGGA GGAGGGCAAG 
GGCCTCTTCA CCTGCCGTAT GAACATCCTG GGTCACATGC AGCAGGGCGG CTCACCCACT 
CCCTTCGACC GCAACATGGG CACCAAAATG GCCGCCAAGT GTGTCGACTG GCTGGCCGCC 
CAGATCAAGG CGAACATCGA CGCCAACGGC GTAGTCAACT GCAAGTCCCC GGACACCGCC 
ACGCTGCTGG GAATCGTGTC GCGGCAGTAC CGCTTCTCAC CGCTGGTCGA CCTCATTGCA 
GAGACGAACT TCGATCAACG CATCCCGAAG AAGCAGTGGT GGCTGCGCCT GCGTCCCCTG 
CTGCGCATCC TGGCCAAGCA CGATTCCGCC TACGAGGAGG AGGGTATGTA CATCACCGTC 
GAGGAGGAGT GTGACACTGA CGCCGTCGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246234.1
CDS	103..2955
Translation	MHSIKFRVFTKLKPIFLEINGRIPICRHFHGPTTFRLEISNKTPPIRQKLTFPNIGIQCTRSHHLCCPRDISGNTLLSVKFNCKRHCIKLRSDSGDQKNDSPGEKNIQKDKSAQRCGKPINNLHNGFLNAVNYSEKNAVKKKKSAPKRKCGKSVDELRKCLRTMQDVIDFVHPVKPFKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLSVVAGIISEADYVFLPESPPQADWPDRLVLKLEQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA

Target ORF information:

RefSeq Version	NM_001259305.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphofructokinase, transcript variant D (Pfk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259305.1

ORF Insert Sequence:

ATGCATTCAA TAAAATTTCG AGTATTTACC AAATTAAAAC CTATATTTTT GGAAATCAAT 
GGACGGATAC CCATTTGCCG CCATTTTCAT GGTCCTACAA CATTTCGCTT AGAAATCTCA 
AACAAAACAC CTCCGATTAG GCAAAAGCTC ACATTTCCAA ACATCGGCAT CCAATGCACA 
CGAAGTCATC ATTTGTGTTG TCCTCGAGAT ATTTCAGGAA ATACTCTTTT AAGTGTAAAA 
TTTAATTGCA AACGACATTG TATAAAGTTG CGCAGTGATT CTGGTGATCA AAAGAATGAT 
AGTCCTGGTG AAAAAAACAT ACAAAAAGAC AAGTCTGCGC AACGATGTGG AAAACCAATA 
AACAATCTGC ACAACGGATT TCTTAATGCT GTGAACTATT CTGAAAAGAA TGCGGTGAAA 
AAGAAAAAGA GTGCACCAAA AAGGAAATGT GGCAAATCGG TGGATGAGCT GCGAAAGTGT 
TTAAGAACCA TGCAGGATGT TATTGATTTT GTACATCCAG TCAAGCCTTT TAAGGACAAA 
GGACTGGCGG TCTTCACCAG CGGCGGGGAT TCCCAGGGCA TGAATGCCGC CGTGCGAGCC 
TGTGTCCGTA TGGCCATCTA TTTGGGCTGC AAGGTCTACT TCATTCGTGA AGGCTACCAG 
GGCATGGTGG ATGGTGGCGA CTGCATCCAG GAAGCCAACT GGGCCTCAGT CTCATCCATT 
ATCCATCGTG GTGGCACCAT CATTGGCTCC GCCCGTTGCC AGGACTTCCG TGAGCGTCAG 
GGTCGCTTGA AAGCCGCTAA CAACCTGATT CAGCGAGGAA TCACCAATCT GGTGGTCATT 
GGAGGCGATG GCTCCCTCAC GGGCGCCAAT CTGTTCCGTC AGGAGTGGTC CAGCCTGTTG 
GACGAGCTGG TCAAGAACAA GACCATTACC ACCGAGCAGC AGGAGAAGTT CAATGTCCTG 
CACATCGTTG GATTGGTTGG CTCCATCGAC AACGATTTCT GTGGCACTGA CATGACCATC 
GGCACGGACA CGGCACTACA TCGCATCATC GAGGCAATCG ATGCCATCTC CAGTACAGCC 
TACTCCCATC AGCGCACCTT CATCATGGAG GTCATGGGTC GTCATTGCGG CTATCTTTCG 
GTGGTAGCTG GCATTATCTC CGAGGCAGAC TACGTCTTTC TGCCCGAGTC TCCTCCGCAG 
GCCGATTGGC CCGATCGACT TGTCCTTAAA TTGGAACAGG AGCGATCTGC TGGCCAGCGT 
CTGAACATCG TGATTGTGGC CGAGGGAGCC ATGGATCGCG AGGGTCATCC CATCACAGCC 
GAGGATGTAA AGAAGGTGAT CGACGAGCGT CTGAAGCACG ATGCCCGCAT CACTGTCTTG 
GGTCACGTGC AGCGCGGTGG CAATCCCAGT GCCTTCGATC GTATTTTGGC TTGCCGCATG 
GGAGCTGAGG CCACTTTGGC CCTGATGGAG GCCACCAAGG ACTCGGTGCC AGTGGTCATC 
TCTCTGGACG GCAACCAGGC GGTTCGCGTG CCGCTGATGG AGTGCGTAGA GCGCACCCAG 
GCTGTGGCCA AGGCCATGGC GGAGAAACGC TGGGCGGATG CCGTCAAGCT GCGCGGACGC 
TCCTTCGAGC GGAATCTGGA GACCTACAAG ATGCTGACGC GCTTGAAGCC GCCCAAGGAG 
AACTTCGACG CCGATGGCAA GGGAATCGAA GGATACCGCC TAGCTGTGAT GCATATTGGT 
GCTCCGGCTT GCGGTATGAA TGCCGCTGTG CGCAGCTTCG TGCGCAATGC CATCTACCGC 
GGTGATGTGG TTTATGGAAT CAACGACGGT GTCGAGGGTC TGATTGCCGG AAATGTCCGC 
GAGCTGGGCT GGTCGGATGT CTCAGGATGG GTTGGTCAGG GTGGCGCCTA CTTGGGTACC 
AAACGCACAC TGCCTGAGGG CAAGTTCAAG GAGATCGCCG CTCGTCTCAA GGAGTTTAAG 
ATCCAGGGTC TCCTGATCAT TGGTGGCTTT GAGAGTTACC ATGCCGCCGG ACAGATCGCC 
GATCAGCGGG ACAACTACCC ACAGTTCTGC ATCCCCATTG TGGTTATTCC ATCGACGATT 
TCGAACAATG TGCCCGGCAC AGAATTTTCT CTGGGATGCG ACACCGGTTT AAATGAGATT 
ACGGAGATTT GCGACCGTAT CCGTCAGTCG GCACAGGGAA CCAAGCGCCG AGTGTTCGTC 
ATTGAGACGA TGGGTGGCTA CTGTGGCTAT TTGGCCACCT TGGCCGGCTT GGCCGGCGGA 
GCTGATGCCG CCTACATCTA CGAGGAGAAG TTCTCCATCA AAGACCTGCA GCAGGATGTC 
TACCACATGG CCTCCAAGAT GGCCGAGGGC GTCTCCCGCG GTCTAATCCT GCGAAACGAG 
AAGGCTAGCG AGAACTACAG CACGGACTTC ATTTACCGCC TGTACTCGGA GGAGGGCAAG 
GGCCTCTTCA CCTGCCGTAT GAACATCCTG GGTCACATGC AGCAGGGCGG CTCACCCACT 
CCCTTCGACC GCAACATGGG CACCAAAATG GCCGCCAAGT GTGTCGACTG GCTGGCCGCC 
CAGATCAAGG CGAACATCGA CGCCAACGGC GTAGTCAACT GCAAGTCCCC GGACACCGCC 
ACGCTGCTGG GAATCGTGTC GCGGCAGTAC CGCTTCTCAC CGCTGGTCGA CCTCATTGCA 
GAGACGAACT TCGATCAACG CATCCCGAAG AAGCAGTGGT GGCTGCGCCT GCGTCCCCTG 
CTGCGCATCC TGGCCAAGCA CGATTCCGCC TACGAGGAGG AGGGTATGTA CATCACCGTC 
GAGGAGGAGT GTGACACTGA CGCCGTCGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pfk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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