Pka-R2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pka-R2
Entrez Gene ID	36041
Full Name	Protein kinase, cAMP-dependent, regulatory subunit type 2
Synonyms	BcDNA:GM01761,CG15862,Cos,Cos-1,Cos1,Dmel\CG15862,Epa,PKA,PKA RII,PKA-R2,PKA-RII,PKa-R2,Pka-RII,RII,RII[[DR]],cos1,pkA,pka-RII
General protein information	Preferred Names Protein kinase, cAMP-dependent, regulatory subunit type 2 Names CG15862 gene product from transcript CG15862-RB CG15862-PA CG15862-PB CG15862-PD CG15862-PE Costal-1 Pka-R2-PA Pka-R2-PB Pka-R2-PD Pka-R2-PE cAMP-dependent protein kinase R2 cAMP-dependent protein kinase type II costal epaulette protein kinase A protein kinase, cAMP-dependent, regulatory subunit type 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46D1-46D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_165723.4	NP_724860.2	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform B
NM_001103781.3	NP_001097251.1	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform D
NM_001103782.2	NP_001097252.1	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform E
NM_078947.4	NP_523671.1	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRKAR2A	XP_003432928.1
Bos taurus (cattle)	PRKAR2A	NP_001178296.1
Gallus gallus (chicken)	LOC100858827	XP_003642054.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004940	XP_315029.3
Eremothecium gossypii	AGOS_ADL099C	NP_983997.1
Mus musculus (house mouse)	Prkar2a	NP_032950.1
Rattus norvegicus (Norway rat)	Prkar2a	NP_062137.1
Drosophila melanogaster (fruit fly)	Pka-R2	NP_724860.2
Neurospora crassa	NCU01166	XP_961532.1
Homo sapiens (human)	PRKAR2A	NP_004148.1
Kluyveromyces lactis	KLLA0E04181g	XP_454132.1
Xenopus tropicalis (tropical clawed frog)	prkar2a	XP_002933104.1
Danio rerio (zebrafish)	prkar2aa	NP_001070838.2
Saccharomyces cerevisiae (baker's yeast)	BCY1	NP_012231.1
	LOC708981	XP_002808369.1
Schizosaccharomyces pombe (fission yeast)	cgs1	NP_594274.1
Magnaporthe oryzae (rice blast fungus)	MGG_07335	XP_003715572.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of Cos1 appear to be dominant-negative mutations of a catalytic subunit of protein kinase A
Title: Drosophila Costal1 mutations are alleles of protein kinase A that modulate hedgehog signaling.

The following Pka-R2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pka-R2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09437	NM_165723.4 Latest version!	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant B (Pka-R2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09437	NM_078947.4 Latest version!	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant A (Pka-R2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09438	NM_001103781.3 Latest version!	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant D (Pka-R2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09439	NM_001103782.2 Latest version!	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant E (Pka-R2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pka-R2 ( NM_165723.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724860.2 Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant B (Pka-R2), mRNA.

Price & Availability↑

CloneID	OFa09437
Clone ID Related Accession (Same CDS sequence)	NM_165723.4 , NM_078947.4
Accession Version	NM_165723.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165723.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTCGA TGTCAAGGCC 
TTTGAGCGTT TGCTGGGTCC CTGCATGGAT ATTATGAAGC GCAACATTGA CGACTACGAG 
TCGCAGTTGG TGAAGATATT CGGCAGCAAA AACAATATCA CCGATACACG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724860.2
CDS	374..1507
Translation	MSSDSSRRIQVPEELKEVLLQFSISFLVEQPPDVIDYAVEYFTKLQSERPSVSHTDQSTDDQLSVNSQDADAEPPVMASSRRKSVFAEAYDPEADDDDDGATAVFPKTDEQRARLVESVKNVLLFRSLEKEQMNQVLDAMFERKVQPGDFIIRQGDDGDNFYVIESGVYKVYINDKHINTYNHTGLFGELALLYNMPRAATVQAETSGLLWAMDRQTFRRILLKSAFRKRKMYEELLNSVPMLKALQNYERMNLADALVSKSYDNGERIIKQGDAADGMYFIEEGTVSVRMDQDDAEVEISQLGKGQYFGELALVTHRPRAASVYATGGVVKLAFLDVKAFERLLGPCMDIMKRNIDDYESQLVKIFGSKNNITDTR

Target ORF information:

RefSeq Version	NM_165723.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant B (Pka-R2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165723.4

ORF Insert Sequence:

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTCGA TGTCAAGGCC 
TTTGAGCGTT TGCTGGGTCC CTGCATGGAT ATTATGAAGC GCAACATTGA CGACTACGAG 
TCGCAGTTGG TGAAGATATT CGGCAGCAAA AACAATATCA CCGATACACG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pka-R2 ( NM_078947.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523671.1 Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant A (Pka-R2), mRNA.

Price & Availability↑

CloneID	OFa09437
Clone ID Related Accession (Same CDS sequence)	NM_165723.4 , NM_078947.4
Accession Version	NM_078947.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078947.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTCGA TGTCAAGGCC 
TTTGAGCGTT TGCTGGGTCC CTGCATGGAT ATTATGAAGC GCAACATTGA CGACTACGAG 
TCGCAGTTGG TGAAGATATT CGGCAGCAAA AACAATATCA CCGATACACG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523671.1
CDS	353..1486
Translation	MSSDSSRRIQVPEELKEVLLQFSISFLVEQPPDVIDYAVEYFTKLQSERPSVSHTDQSTDDQLSVNSQDADAEPPVMASSRRKSVFAEAYDPEADDDDDGATAVFPKTDEQRARLVESVKNVLLFRSLEKEQMNQVLDAMFERKVQPGDFIIRQGDDGDNFYVIESGVYKVYINDKHINTYNHTGLFGELALLYNMPRAATVQAETSGLLWAMDRQTFRRILLKSAFRKRKMYEELLNSVPMLKALQNYERMNLADALVSKSYDNGERIIKQGDAADGMYFIEEGTVSVRMDQDDAEVEISQLGKGQYFGELALVTHRPRAASVYATGGVVKLAFLDVKAFERLLGPCMDIMKRNIDDYESQLVKIFGSKNNITDTR

Target ORF information:

RefSeq Version	NM_078947.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant A (Pka-R2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078947.4

ORF Insert Sequence:

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTCGA TGTCAAGGCC 
TTTGAGCGTT TGCTGGGTCC CTGCATGGAT ATTATGAAGC GCAACATTGA CGACTACGAG 
TCGCAGTTGG TGAAGATATT CGGCAGCAAA AACAATATCA CCGATACACG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pka-R2 ( NM_001103781.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097251.1 Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant D (Pka-R2), mRNA.

Price & Availability↑

CloneID	OFa09438
Clone ID Related Accession (Same CDS sequence)	NM_001103781.3
Accession Version	NM_001103781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103781.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTAGA CACAGAGGCC 
TTTGAGCGCA TCATGGGCTT CCTGACCGAT GTACTCAAGC GCAATATCGT GATCTATGAG 
CAAATGTTCA CCGACATGGC CCGCCGTAAT ACGAATTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097251.1
CDS	353..1474
Translation	MSSDSSRRIQVPEELKEVLLQFSISFLVEQPPDVIDYAVEYFTKLQSERPSVSHTDQSTDDQLSVNSQDADAEPPVMASSRRKSVFAEAYDPEADDDDDGATAVFPKTDEQRARLVESVKNVLLFRSLEKEQMNQVLDAMFERKVQPGDFIIRQGDDGDNFYVIESGVYKVYINDKHINTYNHTGLFGELALLYNMPRAATVQAETSGLLWAMDRQTFRRILLKSAFRKRKMYEELLNSVPMLKALQNYERMNLADALVSKSYDNGERIIKQGDAADGMYFIEEGTVSVRMDQDDAEVEISQLGKGQYFGELALVTHRPRAASVYATGGVVKLAFLDTEAFERIMGFLTDVLKRNIVIYEQMFTDMARRNTNL

Target ORF information:

RefSeq Version	NM_001103781.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant D (Pka-R2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103781.3

ORF Insert Sequence:

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTAGA CACAGAGGCC 
TTTGAGCGCA TCATGGGCTT CCTGACCGAT GTACTCAAGC GCAATATCGT GATCTATGAG 
CAAATGTTCA CCGACATGGC CCGCCGTAAT ACGAATTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pka-R2 ( NM_001103782.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097252.1 Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant E (Pka-R2), mRNA.

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CloneID	OFa09439
Clone ID Related Accession (Same CDS sequence)	NM_001103782.2
Accession Version	NM_001103782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cAMP-dependent, regulatory subunit type 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103782.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTGGA TGTTATTTGC 
TTCGAACGCT TAATGGGAAA GTGTAGTGGG GGCATTCAGC GTAGCATTAG TGGATATCGC 
TATCTGGAAC AGGATCTGCG CGAATATTTC GGCAACTTGC CGCTTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097252.1
CDS	510..1640
Translation	MSSDSSRRIQVPEELKEVLLQFSISFLVEQPPDVIDYAVEYFTKLQSERPSVSHTDQSTDDQLSVNSQDADAEPPVMASSRRKSVFAEAYDPEADDDDDGATAVFPKTDEQRARLVESVKNVLLFRSLEKEQMNQVLDAMFERKVQPGDFIIRQGDDGDNFYVIESGVYKVYINDKHINTYNHTGLFGELALLYNMPRAATVQAETSGLLWAMDRQTFRRILLKSAFRKRKMYEELLNSVPMLKALQNYERMNLADALVSKSYDNGERIIKQGDAADGMYFIEEGTVSVRMDQDDAEVEISQLGKGQYFGELALVTHRPRAASVYATGGVVKLAFLDVICFERLMGKCSGGIQRSISGYRYLEQDLREYFGNLPLN

Target ORF information:

RefSeq Version	NM_001103782.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cAMP-dependent, regulatory subunit type 2, transcript variant E (Pka-R2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103782.2

ORF Insert Sequence:

ATGTCGAGCG ATTCGAGTCG AAGGATCCAG GTGCCCGAGG AGCTGAAGGA GGTGCTGCTG 
CAGTTCTCGA TCTCCTTTCT GGTGGAACAA CCGCCGGATG TGATCGATTA CGCTGTGGAG 
TACTTCACCA AACTGCAGTC GGAACGCCCG AGCGTCTCTC ACACGGATCA AAGCACCGAT 
GACCAGCTGA GCGTCAACTC ACAGGATGCC GATGCGGAAC CACCAGTGAT GGCCAGCTCG 
CGTCGCAAAT CAGTTTTCGC CGAGGCCTAC GATCCGGAGG CGGATGACGA CGATGATGGC 
GCCACCGCCG TGTTCCCCAA GACAGATGAG CAGCGCGCCC GGCTGGTCGA GTCGGTGAAG 
AACGTCCTCC TCTTCCGATC CCTCGAGAAG GAGCAGATGA ACCAAGTCCT GGATGCCATG 
TTCGAGCGAA AGGTTCAGCC GGGTGACTTT ATCATCCGCC AGGGTGACGA CGGCGATAAC 
TTTTATGTTA TTGAATCTGG CGTCTACAAA GTCTATATTA ATGACAAGCA CATTAACACC 
TACAATCACA CTGGACTCTT CGGTGAATTA GCGCTGCTCT ACAATATGCC CAGAGCGGCC 
ACCGTGCAGG CGGAAACGAG TGGTCTACTT TGGGCCATGG ATCGCCAGAC CTTCCGCCGC 
ATCCTCTTGA AGTCGGCCTT CAGGAAGCGG AAAATGTACG AGGAGCTGTT GAACAGCGTG 
CCCATGCTGA AGGCCTTGCA GAACTACGAA CGCATGAATC TGGCGGATGC CTTGGTTTCG 
AAGAGCTACG ACAATGGCGA ACGCATCATC AAACAGGGTG ACGCCGCCGA CGGCATGTAC 
TTCATTGAGG AGGGAACGGT GTCCGTGCGC ATGGACCAGG ACGACGCCGA GGTGGAGATC 
TCCCAGCTGG GCAAGGGACA GTACTTCGGC GAGCTGGCGC TGGTGACACA TCGACCCCGG 
GCAGCATCCG TCTACGCCAC GGGCGGCGTC GTCAAGCTAG CATTCCTGGA TGTTATTTGC 
TTCGAACGCT TAATGGGAAA GTGTAGTGGG GGCATTCAGC GTAGCATTAG TGGATATCGC 
TATCTGGAAC AGGATCTGCG CGAATATTTC GGCAACTTGC CGCTTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pka-R2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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