PCB gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_165711.2	NP_724843.1	pyruvate carboxylase, isoform K
NM_001169632.2	NP_001163103.1	pyruvate carboxylase, isoform M
NM_165709.2	NP_724841.1	pyruvate carboxylase, isoform I
NM_136683.3	NP_610527.1	pyruvate carboxylase, isoform E
NM_165710.3	NP_724842.1	pyruvate carboxylase, isoform J
NM_165705.3	NP_724837.1	pyruvate carboxylase, isoform A
NM_165706.2	NP_724838.1	pyruvate carboxylase, isoform B
NM_165712.2	NP_724844.1	pyruvate carboxylase, isoform L
NM_165708.2	NP_724840.1	pyruvate carboxylase, isoform G
NM_165707.3	NP_724839.1	pyruvate carboxylase, isoform D

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09407	NM_165705.3 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant A (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09407	NM_165708.2 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant G (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09407	NM_165707.3 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant D (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09407	NM_165706.2 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant B (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09407	NM_136683.3 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant E (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09408	NM_165712.2 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant L (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09408	NM_001169632.2 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant M (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09408	NM_165710.3 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant J (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09408	NM_165711.2 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant K (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09408	NM_165709.2 Latest version!	Drosophila melanogaster pyruvate carboxylase, transcript variant I (PCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

PCB ( NM_136683.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610527.1 Drosophila melanogaster pyruvate carboxylase, transcript variant E (PCB), mRNA.

Price & Availability↑

CloneID	OFa09407
Clone ID Related Accession (Same CDS sequence)	NM_136683.3 , NM_165705.3 , NM_165706.2 , NM_165708.2 , NM_165707.3
Accession Version	NM_136683.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_136683.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610527.1
CDS	122..3667
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_136683.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant E (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136683.3

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165705.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724837.1 Drosophila melanogaster pyruvate carboxylase, transcript variant A (PCB), mRNA.

Price & Availability↑

CloneID	OFa09407
Clone ID Related Accession (Same CDS sequence)	NM_136683.3 , NM_165705.3 , NM_165706.2 , NM_165708.2 , NM_165707.3
Accession Version	NM_165705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165705.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724837.1
CDS	419..3964
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165705.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant A (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165705.3

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165706.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724838.1 Drosophila melanogaster pyruvate carboxylase, transcript variant B (PCB), mRNA.

Price & Availability↑

CloneID	OFa09407
Clone ID Related Accession (Same CDS sequence)	NM_136683.3 , NM_165705.3 , NM_165706.2 , NM_165708.2 , NM_165707.3
Accession Version	NM_165706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165706.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724838.1
CDS	164..3709
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165706.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant B (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165706.2

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165708.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724840.1 Drosophila melanogaster pyruvate carboxylase, transcript variant G (PCB), mRNA.

Price & Availability↑

CloneID	OFa09407
Clone ID Related Accession (Same CDS sequence)	NM_136683.3 , NM_165705.3 , NM_165706.2 , NM_165708.2 , NM_165707.3
Accession Version	NM_165708.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165708.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724840.1
CDS	229..3774
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165708.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant G (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165708.2

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165707.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724839.1 Drosophila melanogaster pyruvate carboxylase, transcript variant D (PCB), mRNA.

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CloneID	OFa09407
Clone ID Related Accession (Same CDS sequence)	NM_136683.3 , NM_165705.3 , NM_165706.2 , NM_165708.2 , NM_165707.3
Accession Version	NM_165707.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165707.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724839.1
CDS	297..3842
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165707.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant D (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165707.3

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGT TACGGAACCA 
CCGAAGGGAC TCCGCGAGGT CCTGGTGTGC GAAGGTCCGG AGGCCTTCGC CAAGGAGGTG 
CGCAACCGTA AGGAACTGCT ACTCATGGAC ACCACCTTCC GCGATGCCCA CCAGTCGCTG 
CTGGCCACCC GCGTGCGTTC CCACGATCTG CTGAAGATCT CCCCCTACGT GACGCACAAG 
TTCAACAACC TGTATTCGCT GGAGAACTGG GGCGGAGCCA CCTTCGACGT GGCGCTGCGC 
TTCCTGCACG AGTGCCCGTG GGAGCGCCTG GAGGAGATGC GCAAGCGCAT CCCGAACATT 
CCCTTCCAGA TGCTGTTGCG CGGAGCCAAC GCCGTCGGCT ACACCAGCTA TCCGGACAAC 
GTGGTCTACA AGTTCTGTGA GCTGGCTGTG CAGACCGGCA TGGACATCTT CAGGGTGTTC 
GACTCGCTCA ACTACCTGCC CAACCTGATC CTCGGCATGG AAGCCGCTGG AAAGGCGGGC 
GGCGTGGTGG AGGCTGCCAT CTCCTATACC GGAGACGTCA GCGATCCCAA GCGCACCAAG 
TATGATCTGA AATACTACAC TAACCTTGCC GATGAGCTGG TCAAGGCGGG CACCCACGTG 
CTCTGCATAA AGGATATGGC TGGTCTGCTC AAGCCCGAAT CCGCCAGGCT TCTGATCACA 
GCCATTCGTG ACAAGCACCC CGACATCCCA ATCCATATCC ACACCCACGA CACCTCTGGA 
GCGGGAGTGG CCTCCATGCT GGCATGCGCC AATGCCGGAG CCGATGTGGT GGACGTGGCC 
GTCGACTCCA TGAGCGGCAT GACTTCCCAG CCGAGCATGG GTGCCGTAGT GGCCTCCCTG 
CAAGGCACTC CGCTGGACAC GAACTTGGAT CTGCGCACGG TGTCGGAGTA CTCCGCCTAC 
TGGGAGCAGA CCCGCACCCT GTACGCTCCC TTCGAGTGCA CCACAACAAT GCGGTCGGGC 
AACGCCGATG TCTACCTCAA CGAGATTCCC GGCGGCCAGT ACACCAACCT GCAGTTCCAG 
GCCTTCTCGC TGGGTCTGGG CGACTTCTTC GAGGACGTTA AGAAGGCCTA CCGTGAGGCC 
AACCTGTTGT TGGGTGACAT TATCAAGGTG ACGCCCTCGT CGAAGGTGGT AGGTGATCTT 
GCCCAGTTCA TGGTGCAGAA TGACCTGACC GCCGATCAAG TGCTGGAGCG GGCCGAGGAG 
CTGTCGTTCC CCAAGTCGGT GGTGGAGTAC TTGCAGGGCT CTATTGGCAT TCCGCACGGC 
GGATTCCCCG AGCCGCTGCG TTCGCGAGTG CTCAAGGACA TGCCCCGCAT CGAGGGACGT 
CCGGGAGCCG AACTGAAGGA CTTGGACTTT GACAAGCTGA AGAAGGAACT GCAGGAGTCT 
CACACCTGCG TCACCAACCG TGATGTCATG TCAGCTGCCC TCTACCCGCA GGTGACGAAT 
GATTTCCTAA ACTTCCGCGA AAAGTACGGA CCCGTCGACA AGCTGGACAC TCGCATCTTC 
CTCACGGGTC CCAAGGTGGG CGAGGAGTTC GACGTACCTC TGGAGCGCGG TAAGACGCTG 
AGCGTGAAAG CTCTGGCTGT GTCCGCCGAT CTCAAGCCCA ACGGTATCCG CGAGGTATTC 
TTTGAGTTGA ATGGCCAGCT GCGCGCGGTC CACATCCTGG ACAAGGAAGC CGTTAAGGAG 
ATCCATGTCC ATCCCAAAGC GAACAAGTCG AACAAGAGCG AGGTGGGCGC CCCCATGCCC 
GGAACTGTCA TCGATATCCG CGTGAAGGTT GGCGACAAAG TGGAGAAGGG TCAGCCTCTG 
GTCGTGCTCT CGGCCATGAA GATGGAGATG GTGGTGCAGT CGCCATTGGC GGGCGTTGTC 
AAGAAGCTGG AAATTGCCAA CGGAACGAAG CTGGAGGGCG AGGACCTCAT TATGATTATC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165711.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724843.1 Drosophila melanogaster pyruvate carboxylase, transcript variant K (PCB), mRNA.

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CloneID	OFa09408
Clone ID Related Accession (Same CDS sequence)	NM_165711.2 , NM_001169632.2 , NM_165709.2 , NM_165710.3 , NM_165712.2
Accession Version	NM_165711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165711.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724843.1
CDS	164..3757
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLDLSPEAIEREERGEAKVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165711.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant K (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165711.2

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_001169632.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163103.1 Drosophila melanogaster pyruvate carboxylase, transcript variant M (PCB), mRNA.

Price & Availability↑

CloneID	OFa09408
Clone ID Related Accession (Same CDS sequence)	NM_165711.2 , NM_001169632.2 , NM_165709.2 , NM_165710.3 , NM_165712.2
Accession Version	NM_001169632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169632.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163103.1
CDS	347..3940
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLDLSPEAIEREERGEAKVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_001169632.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant M (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169632.2

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165709.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724841.1 Drosophila melanogaster pyruvate carboxylase, transcript variant I (PCB), mRNA.

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CloneID	OFa09408
Clone ID Related Accession (Same CDS sequence)	NM_165711.2 , NM_001169632.2 , NM_165709.2 , NM_165710.3 , NM_165712.2
Accession Version	NM_165709.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165709.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724841.1
CDS	122..3715
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLDLSPEAIEREERGEAKVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165709.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant I (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165709.2

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165710.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724842.1 Drosophila melanogaster pyruvate carboxylase, transcript variant J (PCB), mRNA.

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CloneID	OFa09408
Clone ID Related Accession (Same CDS sequence)	NM_165711.2 , NM_001169632.2 , NM_165709.2 , NM_165710.3 , NM_165712.2
Accession Version	NM_165710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165710.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724842.1
CDS	419..4012
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLDLSPEAIEREERGEAKVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165710.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant J (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165710.3

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCB ( NM_165712.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724844.1 Drosophila melanogaster pyruvate carboxylase, transcript variant L (PCB), mRNA.

Price & Availability↑

CloneID	OFa09408
Clone ID Related Accession (Same CDS sequence)	NM_165711.2 , NM_001169632.2 , NM_165709.2 , NM_165710.3 , NM_165712.2
Accession Version	NM_165712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate carboxylase, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165712.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724844.1
CDS	229..3822
Translation	MFIPAAQSAYRTLRKTQPRVRLNAIFKNGYSSKVEYKPIRSVLVANRGEIAIRVFRACTELGIKSVAVYSEQDKMHMHRQKADESYIVGKGLPPVEAYLNIPELIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPEVVQKMGDKVAARVAAIEAGVPIVPGTDGPVTTKEEALEFCKKHGLPVIFKAAYGGGGRGMRVVRKMEDVEESFQRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEIRDKMTEAAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQDKIVPRGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSASKMNRALREFRIRGVKTNIPFLLNVLENQKFLHGVLDTYFIDEHPQLFKFKPSLNRAQKLLNYMGEVLVNGPQTPLATTLKPALVSPHVPEVPLDLSPEAIEREERGEAKVTEPPKGLREVLVCEGPEAFAKEVRNRKELLLMDTTFRDAHQSLLATRVRSHDLLKISPYVTHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKRIPNIPFQMLLRGANAVGYTSYPDNVVYKFCELAVQTGMDIFRVFDSLNYLPNLILGMEAAGKAGGVVEAAISYTGDVSDPKRTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPESARLLITAIRDKHPDIPIHIHTHDTSGAGVASMLACANAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTPLDTNLDLRTVSEYSAYWEQTRTLYAPFECTTTMRSGNADVYLNEIPGGQYTNLQFQAFSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNDLTADQVLERAEELSFPKSVVEYLQGSIGIPHGGFPEPLRSRVLKDMPRIEGRPGAELKDLDFDKLKKELQESHTCVTNRDVMSAALYPQVTNDFLNFREKYGPVDKLDTRIFLTGPKVGEEFDVPLERGKTLSVKALAVSADLKPNGIREVFFELNGQLRAVHILDKEAVKEIHVHPKANKSNKSEVGAPMPGTVIDIRVKVGDKVEKGQPLVVLSAMKMEMVVQSPLAGVVKKLEIANGTKLEGEDLIMIIE

Target ORF information:

RefSeq Version	NM_165712.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate carboxylase, transcript variant L (PCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165712.2

ORF Insert Sequence:

ATGTTCATTC CGGCCGCCCA GTCCGCGTAC AGGACGCTGC GCAAGACCCA GCCACGCGTC 
CGGCTCAACG CCATCTTCAA AAATGGCTAC TCCAGCAAGG TGGAGTACAA GCCCATCCGG 
TCAGTGCTGG TGGCTAACCG CGGTGAGATC GCCATCCGAG TGTTCCGTGC CTGCACAGAG 
TTGGGCATCA AATCGGTGGC GGTCTACTCG GAGCAGGATA AGATGCACAT GCACCGACAA 
AAGGCCGACG AGTCGTACAT AGTGGGCAAG GGTCTGCCCC CGGTGGAGGC CTACCTCAAC 
ATTCCAGAGC TAATTAGGGT GTGCAAGGAG AACGATGTGG ATGCCGTGCA TCCCGGCTAC 
GGGTTCCTTT CAGAGCGCAG TGACTTCGCC CAAGCGGTGA TTGATGCTGG ACTGCGCTTC 
ATTGGCCCCT CACCCGAAGT CGTCCAGAAG ATGGGTGATA AGGTGGCGGC TCGTGTGGCG 
GCCATTGAAG CGGGTGTGCC CATTGTGCCC GGCACCGACG GTCCAGTGAC CACCAAGGAG 
GAGGCACTGG AGTTCTGCAA GAAGCACGGA CTGCCCGTTA TCTTCAAGGC CGCTTACGGA 
GGCGGAGGTC GCGGCATGCG CGTCGTCCGC AAAATGGAGG ATGTGGAGGA GAGCTTCCAG 
CGAGCCAGTT CCGAGGCCAA GGCCGCCTTT GGCAATGGTG CGATGTTCAT TGAGAAGTTC 
ATCGAGCGAC CGCGTCACAT TGAGGTTCAA CTGCTGGGAG ACAAGGCCGG GAATGTAGTG 
CATCTGTACG AACGTGACTG CTCCGTGCAG CGTCGCCACC AGAAGGTGGT GGAGATCGCC 
CCAGCGCCAC GCCTGCCCAT CGAAATCCGG GACAAGATGA CGGAGGCAGC AGTGCGCTTG 
GCCCGCCATG TGGGCTATGA AAACGCCGGA ACCGTGGAGT TCCTTTGCGA CGAGTCCGGC 
AACTTCTACT TCATCGAAGT GAACGCCCGT CTACAAGTGG AGCACACGGT CACCGAGGAG 
ATAACAGGCA TCGATTTGGT GCAGTCGCAA ATCCGCGTGG CCGAGGGCAT GACCCTACCC 
GAATTGGGCT ACACGCAGGA CAAGATCGTG CCGCGTGGCT ATGCCATTCA GTGCCGTGTG 
ACCACCGAAG ATCCGGCCAA CGATTTTCAG CCCAACACCG GTCGCTTGGA GGTCTTCCGA 
TCTGGCGAGG GTATGGGCAT TAGGTTGGAC AGTGCGTCCG CCTACGCCGG AGCCATCATT 
TCGCCATACT ACGACTCCCT GCTGGTCAAG GTGATCTCGC ACGCCAGCGA TCTGCAGAGC 
TCCGCCTCCA AGATGAACCG TGCCCTAAGG GAATTCCGCA TTCGCGGCGT CAAGACCAAT 
ATCCCCTTCC TCCTAAACGT GCTGGAGAAC CAGAAGTTCC TCCACGGCGT TCTCGACACC 
TACTTCATCG ACGAGCACCC GCAACTGTTC AAGTTCAAGC CCTCGCTCAA TCGCGCCCAG 
AAGCTGCTCA ACTATATGGG TGAGGTGCTG GTCAACGGAC CCCAGACTCC TCTCGCCACC 
ACTCTGAAGC CTGCCCTCGT CTCGCCCCAC GTTCCCGAGG TTCCGCTGGA TCTGTCCCCG 
GAAGCTATAG AACGCGAAGA ACGTGGCGAG GCAAAAGTTA CGGAACCACC GAAGGGACTC 
CGCGAGGTCC TGGTGTGCGA AGGTCCGGAG GCCTTCGCCA AGGAGGTGCG CAACCGTAAG 
GAACTGCTAC TCATGGACAC CACCTTCCGC GATGCCCACC AGTCGCTGCT GGCCACCCGC 
GTGCGTTCCC ACGATCTGCT GAAGATCTCC CCCTACGTGA CGCACAAGTT CAACAACCTG 
TATTCGCTGG AGAACTGGGG CGGAGCCACC TTCGACGTGG CGCTGCGCTT CCTGCACGAG 
TGCCCGTGGG AGCGCCTGGA GGAGATGCGC AAGCGCATCC CGAACATTCC CTTCCAGATG 
CTGTTGCGCG GAGCCAACGC CGTCGGCTAC ACCAGCTATC CGGACAACGT GGTCTACAAG 
TTCTGTGAGC TGGCTGTGCA GACCGGCATG GACATCTTCA GGGTGTTCGA CTCGCTCAAC 
TACCTGCCCA ACCTGATCCT CGGCATGGAA GCCGCTGGAA AGGCGGGCGG CGTGGTGGAG 
GCTGCCATCT CCTATACCGG AGACGTCAGC GATCCCAAGC GCACCAAGTA TGATCTGAAA 
TACTACACTA ACCTTGCCGA TGAGCTGGTC AAGGCGGGCA CCCACGTGCT CTGCATAAAG 
GATATGGCTG GTCTGCTCAA GCCCGAATCC GCCAGGCTTC TGATCACAGC CATTCGTGAC 
AAGCACCCCG ACATCCCAAT CCATATCCAC ACCCACGACA CCTCTGGAGC GGGAGTGGCC 
TCCATGCTGG CATGCGCCAA TGCCGGAGCC GATGTGGTGG ACGTGGCCGT CGACTCCATG 
AGCGGCATGA CTTCCCAGCC GAGCATGGGT GCCGTAGTGG CCTCCCTGCA AGGCACTCCG 
CTGGACACGA ACTTGGATCT GCGCACGGTG TCGGAGTACT CCGCCTACTG GGAGCAGACC 
CGCACCCTGT ACGCTCCCTT CGAGTGCACC ACAACAATGC GGTCGGGCAA CGCCGATGTC 
TACCTCAACG AGATTCCCGG CGGCCAGTAC ACCAACCTGC AGTTCCAGGC CTTCTCGCTG 
GGTCTGGGCG ACTTCTTCGA GGACGTTAAG AAGGCCTACC GTGAGGCCAA CCTGTTGTTG 
GGTGACATTA TCAAGGTGAC GCCCTCGTCG AAGGTGGTAG GTGATCTTGC CCAGTTCATG 
GTGCAGAATG ACCTGACCGC CGATCAAGTG CTGGAGCGGG CCGAGGAGCT GTCGTTCCCC 
AAGTCGGTGG TGGAGTACTT GCAGGGCTCT ATTGGCATTC CGCACGGCGG ATTCCCCGAG 
CCGCTGCGTT CGCGAGTGCT CAAGGACATG CCCCGCATCG AGGGACGTCC GGGAGCCGAA 
CTGAAGGACT TGGACTTTGA CAAGCTGAAG AAGGAACTGC AGGAGTCTCA CACCTGCGTC 
ACCAACCGTG ATGTCATGTC AGCTGCCCTC TACCCGCAGG TGACGAATGA TTTCCTAAAC 
TTCCGCGAAA AGTACGGACC CGTCGACAAG CTGGACACTC GCATCTTCCT CACGGGTCCC 
AAGGTGGGCG AGGAGTTCGA CGTACCTCTG GAGCGCGGTA AGACGCTGAG CGTGAAAGCT 
CTGGCTGTGT CCGCCGATCT CAAGCCCAAC GGTATCCGCG AGGTATTCTT TGAGTTGAAT 
GGCCAGCTGC GCGCGGTCCA CATCCTGGAC AAGGAAGCCG TTAAGGAGAT CCATGTCCAT 
CCCAAAGCGA ACAAGTCGAA CAAGAGCGAG GTGGGCGCCC CCATGCCCGG AACTGTCATC 
GATATCCGCG TGAAGGTTGG CGACAAAGTG GAGAAGGGTC AGCCTCTGGT CGTGCTCTCG 
GCCATGAAGA TGGAGATGGT GGTGCAGTCG CCATTGGCGG GCGTTGTCAA GAAGCTGGAA 
ATTGCCAACG GAACGAAGCT GGAGGGCGAG GACCTCATTA TGATTATCGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PCB
Entrez Gene ID	36020
Full Name	Pyruvate carboxylase
Synonyms	BcDNA,BcDNA:GH06348,CG1516,Dmel\CG1516,PC,Q0E9E2_DROME,dPC
General protein information	Preferred Names Pyruvate carboxylase Names CG1516 gene product from transcript CG1516-RE CG1516-PA CG1516-PB CG1516-PD CG1516-PE CG1516-PG CG1516-PI CG1516-PJ CG1516-PK CG1516-PL CG1516-PM GH06348 PCB-PA PCB-PB PCB-PD PCB-PE PCB-PG PCB-PI PCB-PJ PCB-PK PCB-PL PCB-PM pyruvate carboxykinase pyruvate carboxylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46B4-46C1

Homo sapiens (human)	PC	NP_071504.2
Canis lupus familiaris (dog)	PC	XP_540825.2
Bos taurus (cattle)	PC	NP_808815.2
Mus musculus (house mouse)	Pcx	NP_001156418.1
Caenorhabditis elegans (roundworm)	pyc-1	NP_001256376.1
Saccharomyces cerevisiae (baker's yeast)	PYC2	NP_009777.1
Kluyveromyces lactis	KLLA0C05764g	XP_452456.1
Eremothecium gossypii	AGOS_AAR162C	NP_982705.2
Magnaporthe oryzae (rice blast fungus)	MGG_07756	XP_003712969.1
Pan troglodytes (chimpanzee)	PC	XP_003952003.1
Neurospora crassa	NCU02505	XP_965636.1
Xenopus tropicalis (tropical clawed frog)	pc.2	NP_001096495.1
Schizosaccharomyces pombe (fission yeast)	pyr1	NP_595900.1
	PC	XP_001107814.1
Rattus norvegicus (Norway rat)	Pc	NP_036876.2
Danio rerio (zebrafish)	pcxb	NP_571625.1
Drosophila melanogaster (fruit fly)	CG1516	NP_724844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004742	XP_001689096.1
Saccharomyces cerevisiae (baker's yeast)	PYC1	NP_011453.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PCB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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