Orc6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Orc6
Entrez Gene ID	36017
Full Name	Origin recognition complex subunit 6
Synonyms	CG1584,DmORC 6,DmORC6,Dmel\CG1584,ORC,ORC6,orc6,rDmORC
General protein information	Preferred Names Origin recognition complex subunit 6 Names CG1584 gene product from transcript CG1584-RA CG1584-PA Orc6-PA origin recognition complex subunit 6
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46B4-46B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057971.6	NP_477319.1	origin recognition complex subunit 6

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011246	XP_309400.4
Drosophila melanogaster (fruit fly)	Orc6	NP_477319.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In order to study the effects of Meier-Gorlin syndrome (MGS) mutation in an animal model system, MGS mutation in Orc6 was introduced and established Drosophila model of MGS.
Title: Drosophila model of Meier-Gorlin syndrome based on the mutation in a conserved C-Terminal domain of Orc6.

Drosophila Orc6 protein, the smallest subunit of the origin recognition complex (ORC), directly binds to septin complex and facilitates septin filament formation.
Title: Functional insight into the role of Orc6 in septin complex filament formation in Drosophila.

In a cross-species complementation experiment, the expression of human Orc6 in Drosophila Orc6 mutant cells rescued DNA replication, suggesting that this function of the protein is conserved among metazoans.
Title: Functional analysis of an Orc6 mutant in Drosophila.

These results show that the interaction of Orc6 with the septin complex is dependent on the coiled-coil domain of Pnut, and suggest an active role for Orc6 in septin complex function.
Title: Drosophila Orc6 facilitates GTPase activity and filament formation of the septin complex.

These studies provide a molecular analysis for the functional requirement of Orc6 in replicative functions of ORC in Drosophila and suggest that Orc6 may contribute to the sequence preferences of ORC in targeting to the origins.
Title: Role of the Orc6 protein in origin recognition complex-dependent DNA binding and replication in Drosophila melanogaster.

The following Orc6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Orc6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09404	NM_057971.6 Latest version!	Drosophila melanogaster origin recognition complex subunit 6 (Orc6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Orc6 ( NM_057971.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477319.1 Drosophila melanogaster origin recognition complex subunit 6 (Orc6), mRNA.

Price & Availability↑

CloneID	OFa09404
Clone ID Related Accession (Same CDS sequence)	NM_057971.6
Accession Version	NM_057971.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	origin recognition complex subunit 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057971.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTACCT TAATAGAACA GTTAATTACG AAAATGGGCC TAAGGGAGGA GCCAAACGTG 
CTAGAGAAAA CCACCGAACT AGTACGCCTC CTGGAACTGC GCTCCACGAA TGTCCCTCTG 
CAGATCAACG AGTACGGAAA GATAGTGCTG TGTGCGGATC TCGCTTCCTG CATGATTGGG 
ATCGCTTTCG ACAAGGAGCA GGCACTGAAA TTGTCAGGAT TGCGAAAGAG CCAATACCTC 
AACAACAAGC GGATGTTCGA GAAGCTACTG GACCTAAACA AACTGGCTAG CGTGAATGAC 
ATCTGTGTCC AGCTGGGTCT CAACGAAGTT GCCCGCAAGG CGGAAGAGCT AATGACTCTC 
TTCAAGGGCG TAGCGGCCAC CGAGGACATG GGTACCGACA CCAGCCATCC TCAGTACGCC 
ACCATGGCCG TGTTCCAGGC CTGCCGCCTG CTCAAAAAGA AGGTTTCGAA ATCAAAGCTC 
ATGCCCTTCA GTAATTTGCG ACCCTCCCAG TTTCAACTGC TCGAGCAGCA GTGGGAACGC 
ATGATAGCTA AGCACCACAA GGAGAGCAAA GTACCATCTA GCACCGATAT GGAGGGTAAA 
CTTAAGGAGA ACCAGAATGA GAATATCAAG GGTCATGAGG CAAAAAAGGC ACATAAGCCC 
CCACCGGAGG ACTACGAAAT ATGGAAGGCG CGAATGTTGG CCAAGGCGCA GGCCAAATTG 
AAGGAATTAG AAGCATCCCA ATCGCATATG GATAGCCAGC TTCTCGAGGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477319.1
CDS	92..865
Translation	MTTLIEQLITKMGLREEPNVLEKTTELVRLLELRSTNVPLQINEYGKIVLCADLASCMIGIAFDKEQALKLSGLRKSQYLNNKRMFEKLLDLNKLASVNDICVQLGLNEVARKAEELMTLFKGVAATEDMGTDTSHPQYATMAVFQACRLLKKKVSKSKLMPFSNLRPSQFQLLEQQWERMIAKHHKESKVPSSTDMEGKLKENQNENIKGHEAKKAHKPPPEDYEIWKARMLAKAQAKLKELEASQSHMDSQLLEA

Target ORF information:

RefSeq Version	NM_057971.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster origin recognition complex subunit 6 (Orc6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057971.6

ORF Insert Sequence:

ATGACTACCT TAATAGAACA GTTAATTACG AAAATGGGCC TAAGGGAGGA GCCAAACGTG 
CTAGAGAAAA CCACCGAACT AGTACGCCTC CTGGAACTGC GCTCCACGAA TGTCCCTCTG 
CAGATCAACG AGTACGGAAA GATAGTGCTG TGTGCGGATC TCGCTTCCTG CATGATTGGG 
ATCGCTTTCG ACAAGGAGCA GGCACTGAAA TTGTCAGGAT TGCGAAAGAG CCAATACCTC 
AACAACAAGC GGATGTTCGA GAAGCTACTG GACCTAAACA AACTGGCTAG CGTGAATGAC 
ATCTGTGTCC AGCTGGGTCT CAACGAAGTT GCCCGCAAGG CGGAAGAGCT AATGACTCTC 
TTCAAGGGCG TAGCGGCCAC CGAGGACATG GGTACCGACA CCAGCCATCC TCAGTACGCC 
ACCATGGCCG TGTTCCAGGC CTGCCGCCTG CTCAAAAAGA AGGTTTCGAA ATCAAAGCTC 
ATGCCCTTCA GTAATTTGCG ACCCTCCCAG TTTCAACTGC TCGAGCAGCA GTGGGAACGC 
ATGATAGCTA AGCACCACAA GGAGAGCAAA GTACCATCTA GCACCGATAT GGAGGGTAAA 
CTTAAGGAGA ACCAGAATGA GAATATCAAG GGTCATGAGG CAAAAAAGGC ACATAAGCCC 
CCACCGGAGG ACTACGAAAT ATGGAAGGCG CGAATGTTGG CCAAGGCGCA GGCCAAATTG 
AAGGAATTAG AAGCATCCCA ATCGCATATG GATAGCCAGC TTCTCGAGGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Orc6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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